petrelli curriculum



petrelli curriculum
First name and surname:
Place and date of birth:
Work Address:
Institute for Cancer Research and Treatment, Candiolo
[email protected]
1997: Degree in Biological Science, University of Turin; grades: 110/110 summa cum laude
2001: PhD in Human Genetics, Department of Genetic, Biology and Biochemistry, University of Turin
Research experience:
2011-present: contract work with the Fondazione Piemontese per l’Oncologia
2010: contract work with the Dep. Oncological Science
2009: Contract work with the Fondazione Piemontese per la Ricerca sul Cancro
2008: Fellowship for Scientific Research from the University of Turin
2005: Fellowship from the Italian Association for Cancer Research
2004: Post-doc fellowship from the University of Turin
2002: Fellowship for Scientific Research from the University of Turin
Academic years from 2002-2003 to 2004-2005: collaboration in academic teaching for Histology in the courses of
Scuole di Specialità di Cardiologia e Cardiochirurgia with Prof. Silvia Giordano.
Academic years from 2001-2002 to 2010-2011: collaboration in academic teaching for Histology in the courses of
Facolta’ di Medicina e Chirurgia dell’Universita’ degli Studi di Torino (Ospedale S.Luigi) with Prof. Silvia Giordano.
Academic years from 2010-2011 al 2013-2014: collaboration in academic teaching for Histology in the Corso di Laurea
triennale di Tecnica della Riabilitazione Psichiatrica with Prof. Silvia Giordano.
Technical skills and Competences:
Well-established expertise in: 1) molecular biology: cloning in plasmid and viral vectors, DNA amplification by PCR,
gene expression analysis by quantitative real-time PCR and Northern blot; 2) cell biology: cell culture, transient
transfection, stable transduction with lentiviral vector, biological assays (growth and proliferation curves, anchorage
independent growth in soft agar, tubulogenesis in collagene, migration and invasion assays); 3) biochemistry of
proteins: production of recombinant proteins in bacteria, protein extraction from cells and tissues, protein-protein
interaction studies, SDS-PAGE and Western blot. Immunohystochemistry and immunofluorescence techniques; 4)
microRNA manipulation: microRNA extraction from cells and tissues, expression profiling of microRNAs by miRNA
arrays; miRNA expression analysis by TaqMan QRT-PCR and Northern blot; 5) mouse manipulation: use of
immunodeficient animals (CD1 nude and NOD/SCID mice) for in vivo models of tumorigenesis.
Additional Information: Ability to independently design and organize project and experiments, as well as to work as
part of a team. Good interpersonal skills.
Frau C, Loi R, Petrelli A, Perra A, Menegon S, Kowalik MA, Pinna S, Leoni VP, Fornari F, Gramantieri L, LeddaColumbano GM, Giordano S, Columbano A. Local hypothyroidism favours the progression of rat preneoplastic lesions to
HCC. Hepatology 2014. [Epub ahead of print]
Petrelli A., Perra A, Cora D, Sulas P, Menegon S, Manca C, Migliore C, Kowalik MA, Ledda-Columbano GM, Giordano
S, Columbano A. MicroRNA/gene profiling unveils early molecular changes and nuclear factor erythroid related factor 2
(NRF2) activation in a rat model recapitulating human hepatocellular carcinoma (HCC). Hepatology 2014
Katia Cortese, Mark T. Howes, Richard Lundmark, Erica Tagliatti, Paola Bagnato, Annalisa Petrelli, Maria Bono,
Harvey T. McMahon, Robert G. Parton, and Carlo Tacchetti. The HSP90 inhibitor Geldanamycin perturbs endosomal
structure and drives recycling ErbB2 and Transferrin to modified MVBs/lysosomal compartments. Mol Biol Cell. 2013
Jan; 24(2):129-44.
Petrelli A., Perra A., Schernhuber K., Salvi A., Migliore C., Ghiso E., Benetti A., Barlati S., Ledda-Columbano G.M.,
Portolani N., De Petro G., Columbano A., Giordano S. Sequential analysis of multistage hepatocarcinogenesis reveals
that miR-100 and PLK1 dysregulation is an early event maintained along tumor progression. Oncogene 31(42):4517-26
Annalisa Petrelli. “Polypharmacological kinase inhibitors: new hopes for the therapy of cancer”. In “Polypharmacology
in Drug Discovery", Jens-Uwe Peters (ed.), John Wiley & Sons, Ltd. (2012)
Cristina Migliore, Valentina Martin, Vera P. Leoni, Angelo Restivo, Luigi Atzori, Annalisa Petrelli, Claudio Isella, Luigi
Zorcolo, Ivana Sarotto, Giuseppe Casula, Paolo M. Comoglio, Amedeo Columbano, Silvia Giordano. MiR-1
downregulation cooperates with MACC1 in promoting Met overexpression in human colon cancer. Clin Cancer Res
2012 Feb 1;18(3):737-47.
Valabrega G, Capellero S, Cavalloni G, Zaccarello G, Petrelli A, Migliardi G, Milani A, Peraldo-Neia C, Gammaitoni L,
Sapino A, Pecchioni C, Moggio A, Giordano S, Aglietta M, Montemurro F. HER2-positive breast cancer cells resistant to
trastuzumab and lapatinib lose reliance upon HER2 and are sensitive to the multitargeted kinase inhibitor sorafenib.
Breast Cancer Res Treat. 2011 Nov;130(1):29-40. Epub 2010 Dec 9.
Foveau B., Ancot F., Leroy C., Petrelli A., Reiss K., Vingtdeux V., Giordano S, Fafeur V, Tulasne D. Downregulation of
the Met Receptor Tyrosine Kinase by Presenilin-dependent Regulated Intramembrane Proteolysis. Mol Biol Cell.
20(9):2495-507 (2009)
Petrelli A and Valabrega G. Multitarget drugs: the present and the future of cancer therapy. Expert Opin
Pharmacother. 10(4):589-600 (2009)
Cristina Migliore, Annalisa Petrelli, Elena Ghiso, Simona Corso, Lorena Capparuccia, Adriana Eramo, Paolo M.
Comoglio, Silvia Giordano. MicroRNAs impair MET-mediated invasive growth. Cancer Res 68(24):10128-36 (2008)
A. Petrelli and S. Giordano. From single- to multi-target drugs in cancer therapy: when aspecificity becomes an
advantage. Curr Med Chem 15(5):422-432 (2008). Review
Annalisa Petrelli, Paola Circosta, Luisa Granziero, Massimiliano Mazzone, Alberto Pisacane, Paolo M. Comoglio and
Silvia Giordano. Antibody-induced ectodomain shedding mediates HGF receptor down-regulation and hampers
biological activity. Proc Natl Acad Sci USA 103(13):5090-5 (2006)
Giorgio Valabrega, Filippo Montemurro, Ivana Sarotto, Annalisa Petrelli, Patrizia Rubini, Carlo Tacchetti, Massimo
Aglietta, Paolo Maria Comoglio and Silvia Giordano. TGFα expression impairs Trastuzumab-induced HER2 downregulation. Oncogene 4(18):3002-10 (2005)
Petrelli A., Gilestro G., Lanzardo S., Comoglio P.M., Migone N. and Giordano S. The Endophilin/CIN85/Cbl complex
mediates ligand-dependent down-regulation of c-Met. Nature 416(6877):187-90 (2002).
Cestra G, Castagnoli L, Dente L, Minenkova O, Petrelli A, Migone N, Hoffmuller U, Schneider-Mergener J, Cesareni G.
The SH3 domains of endophilin and amphiphysin bind to the proline-rich region of synaptojanin 1 at distinct sites that
display an unconventional binding specificity. J. Biol. Chem. 274(45): 32001-7 (1999)
Giordano S., Corso S., Conrotto P., Petrelli A. and Comoglio P.M. HGF/Met system. Encyclopedia of Hormones