c-erbB2 ONCOPROTEINE IN CANINE AND FELINE MAMMARY

c-erbB2 ONCOPROTEIN IN CANINE AND FELINE MAMMARY
TUMOR
Dr.ssa Luisa Vera Muscatello
XXVIII ciclo di Dottorato
Tutor Dr.ssa Barbara Brunetti
CANINE
FELINE
ID
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
H. DIAGNOSIS
IHC c-erbB2
Solid carcinoma
1
Solid carcinoma
1
Anaplastic carcinoma
1
Tubulo-papillary carcinoma
1
Solid carcinoma
3
Solid carcinoma
1
Solid carcinoma
1
Solid carcinoma
1
Solid carcinoma
3
Tubulo-papillary carcinoma
3
Complex carcinoma
1
Complex carcinoma
2
Tubulo-papillary carcinoma
2
Solid carcinoma
1
Tubulo-papillary carcinoma
2
ID
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
H. DIAGNOSIS
IHC c-erbB2
Tubulo-papillary carcinoma
3
Tubulo-papillary carcinoma
2
Tubulo-papillary carcinoma
2
Tubulo-papillary carcinoma
1
Solid carcinoma
2
Tubulo-papillary carcinoma
3
Tubulo-papillary carcinoma
0
Tubulo-papillary carcinoma
1
Tubulo-papillary carcinoma
2
Tubulo-papillary carcinoma
2
Tubulo-papillary carcinoma
3
Tubulo-papillary carcinoma
1
Solid carcinoma
3
Solid carcinoma
3
Tubulo-papillary carcinoma
3
16
Tubulo-papillary carcinoma
1
16
Tubulo-papillary carcinoma
2
17
18
19
Tubulo-papillary carcinoma
Anaplastic carcinoma
Tubulo-papillary carcinoma
2
2
1
17
18
19
Solid carcinoma
Solid carcinoma
Solid carcinoma
2
2
2
20
Tubulo-papillary carcinoma
2
20
Tubulo-papillary carcinoma
2
21
Tubulo-papillary carcinoma
2
21
Tubulo-papillary carcinoma
0
22
Tubulo-papillary carcinoma
1
22
Tubulo-papillary carcinoma
1
23
Tubulo-papillary carcinoma
2
23
Tubulo-papillary carcinoma
2
24
Tubulo-papillary carcinoma
3
24
Tubulo-papillary carcinoma
1
25
Tubulo-papillary carcinoma
0
25
Tubulo-papillary carcinoma
1
26
Tubulo-papillary carcinoma
0
26
Tubulo-papillary carcinoma
2
27
Tubulo-papillary carcinoma
1
27
Tubulo-papillary carcinoma
1
28
29
30
31
32
33
34
35
36
37
38
Tubulo-papillary carcinoma
Solid carcinoma
Solid carcinoma
Solid carcinoma
Solid carcinoma
Tubulo-papillary carcinoma
Solid carcinoma
Ductal carcinoma
Solid carcinoma
Carcinosarcoma
Tubulo-papillary carcinoma
3
2
2
2
2
2
0
1
2
1
1
28
29
30
31
32
33
34
35
36
37
38
Tubulo-papillary carcinoma
Solid carcinoma
Tubulo-papillary carcinoma
Tubulo-papillary carcinoma
Solid carcinoma
Solid carcinoma
Solid carcinoma
Solid carcinoma
Ductal carcinoma
Ductal carcinoma
Anaplastic carcinoma
1
2
2
1
1
2
2
2
2
0
2
39
40
41
42
43
44
45
Tubulo-papillary carcinoma
Solid carcinoma
Solid carcinoma
Inflammatory carcinoma
2
2
1
1
39
40
41
42
43
44
45
Tubulo-papillary carcinoma
Tubulo-papillary carcinoma
Solid carcinoma
Ductal carcinoma
Tubulo-papillary carcinoma
Tubulo-papillary carcinoma
Tubulo-papillary carcinoma
1
2
1
2
1
2
2
c-erbB2 is a transmembrane receptor with tyrosine-kinase activity,
overexpressed in human, feline and canine mammary carcinoma and
related to cancer development, poor prognosis and target therapy.
Forty-two and 45 canines and felines mammary tumor
were collected. Immunohistochemistry (IHC) with
anti-erbB2 antibody was performed.
Positive staining (score 2 and 3 Herceptest) were
the 50% of canine and the 64,4% of feline carcinomas.
HercepTest TM is a semi-quantitative immunohistochemical
assay for determination of c-erbB-2 oncoprotein overexpression
in breast cancer, approved by Food and Drug Administration.
Tissue Microarray (TMA) is a
high-throughput method,
adopted for simultaneous
molecular profiling of tissue
samples from large patient
cohorts. Validation of the TMA
method was performed:
positivity of c-erbB2 (score
+2, +3) were concordant in 8
out of 10 cases between full
sections versus TMAs.
In the others two cases the positive score is lowered. TMA platform
preserves the molecular profile of CMT and FMT markers, representing
a useful tool for rapid and cost-effective analysis.
PUBLICATIONS and Others…
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5
1
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2
X Congresso Nazionale AIPVet 2013 ho presentato la
comunicazione orale
Accepted by
Journal of
Comparative
Pathology
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3
X Congresso Nazionale AIPVet 2013 poster
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Immunohistochemical positive staining SCORE 3
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4
Future developments quantification of gene
amplification with FISH and CISH
6