Ilario Losito, nato a Molfetta - Dipartimento di Chimica Università

Curriculum del Prof. Ilario Losito
Ilario Losito ha conseguito la laurea in Chimica con lode presso l’Università degli Studi di Bari nel
1994, discutendo una tesi sulla caratterizzazione mediante Spettroscopia Fotoelettronica a Raggi X
(XPS) di polimeri elettrosintetizzati.
Presso la stessa università ha conseguito, nel 1998, il titolo di Dottore di Ricerca in Scienze
Chimiche, occupandosi dello sviluppo e della caratterizzazione di nuovi materiali elettrosintetizzati
da impiegare come elementi di riconoscimento in sensori biomimetici a trasduzione elettrochimica e
microgravimetrica.
Fra il 1998 e il 1999 ha svolto un periodo di ricerca annuale post-dottorato, in qualità di Research
Support Specialist, presso la State University of New York at Buffalo, NY, USA, occupandosi della
caratterizzazione superficiale, mediante le tecniche XPS e SIMS (Spettrometria di Massa di Ioni
Secondari), di polimeri biodegradabili modificati in superficie per favorirne la biocompatibilità.
Dal 1999 al 2001 ha collaborato, in qualità di assegnista, all’attività di ricerca del gruppo del Prof.
P.G. Zambonin presso il Dipartimento di Chimica dell’Università di Bari, dedicandosi
all’applicazione della Cromatografia Liquida-Spettrometria di Massa ad Interfaccia Elettrospray
(LC-ESI-MS)
all’identificazione
di
prodotti
derivanti
da
reazioni
elettrochimiche
(elettropolimerizzazioni) e fotocatalitiche (degradazione di pesticidi mediante processi catalitici
assistiti da radiazione ultravioletta).
Dal Novembre 2001 ricopre il ruolo di Professore Associato in Chimica Analitica presso
l’Università degli Studi “Aldo Moro” di Bari, con incarichi didattici per i Corsi di Laurea Triennale
in Chimica e in Tecnologie Chimiche, per il Corso di Laurea Specialistica in Scienze e Tecnologie
Chimiche e per il Corso di Laurea Magistrale in Scienze Chimiche. E’ inoltre membro del Collegio
dei Docenti del Dottorato in Microbiologia, Sanità e Chimica degli Alimenti della stessa università,
nell’ambito del quale tiene corsi monografici di argomento chimico-analitico.
Il Prof. Losito ha pubblicato 54 articoli su riviste scientifiche nazionali/internazionali, è co-autore di
un capitolo di libro dedicato alla caratterizzazione mediante XPS e SIMS di polimeri ed è coinventore di un brevetto USA sulla sintesi e caratterizzazione di poliesteri biodegradabili fluorurati.
I suoi attuali interessi di ricerca riguardano la caratterizzazione mediante tecnica ESI-MS
accoppiata a tecniche separative mono/bi-dimensionali in fase liquida (HPLC, CapLC, 2D-LC) di
proteine potenzialmente allergeniche presenti in prodotti vinicoli a seguito dei trattamenti di
chiarificazione, lo studio dell’alchilazione di nucleotidi da parte di epossidi a lunga catena alchilica
e l’identificazione dei fosfolipidi di membrana di piastrine e microparticelle derivanti da sangue di
individui affetti da patologie correlate allo stress ossidativo.
1
Elenco delle pubblicazioni
1. C. Malitesta, I. Losito, F. Scordari, E. Schingaro
XPS investigation of titanium in melanites from Monte Vulture (Italy)
Eur. J. Mineral. 7 (1995) 847
2. C. Malitesta, I. Losito, L. Sabbatini, P.G. Zambonin
New findings on polypyrrole chemical structure by XPS coupled to chemical derivatization
labelling
J. Electron. Spectr. Rel. Phen. 76 (1995) 629
3. I. Losito, C. Malitesta, L. Sabbatini, P.G. Zambonin
Pristine and overoxidised polypyrrole by XPS
Surface Science Spectra 3 (1995) 375
4. I. Losito, C.G. Zambonin
Double electropolymer modified platinum electrode to follow the kinetic process
H2O2+ascorbic acid. Influence of the reaction on amperometric biosensor applications
J. Electroanal. Chem. 410 (1996) 181
5. I. Losito, E. De Giglio, C. Malitesta, L. Sabbatini, P.G. Zambonin
Chemical Derivatization-XPS analysis: a global approach to the characterization of polymer
surfaces
Spectroscopy Europe 9/3 (1997) 16
6. D. Centonze, I. Losito, C. Malitesta, F. Palmisano, P.G. Zambonin
Electrochemical immobilisation of enzymes on conducting organic salt electrodes:
characterisation of an oxygen independent and interference-free glucose biosensor
J. Electroanal. Chem. 435 (1997) 103
7. C.G. Zambonin, I. Losito
Kinetic investigation of the reactions connected to the system ascorbate + O2 by
amperometric detection of H2O2 at a modified platinum electrode
Anal. Chem. 69 (1997) 4113
8. C. Malitesta, I. Losito, L. Sabbatini, P.G. Zambonin
Applicability of chemical derivatization - X-ray photoelectron spectroscopy (CD-XPS) to the
characterization of complex matrices: case of electrosynthesised polypyrroles
J. Electron. Spectr. Rel. Phen. 97 (1998) 199
9. L. Sabbatini, L.; De Giglio, E.; Losito, I.; Malitesta, C.
Application oriented polymer surface modifications: How to perform; how to
control
in Current Trends in Analytical Chemistry, editori G.L. Radu, I. Tanase, G.E. Baiulescu,
Volume 1, 1998, pag. 65-76.
10. C. Malitesta, I. Losito, P.G. Zambonin
Molecularly imprinted electrosynthesised polymers: new materials for biomimetic sensors
Anal. Chem. 71 (1999) 1366
11. L. Sabbatini, C. Malitesta, E. De Giglio, I. Losito, L. Torsi, P.G. Zambonin
2
Electrosynthesised thin polymer films: the role of XPS in the design of application
oriented innovative materials
J. Electron Spectr. Rel. Phen. 100 (1999) 35
12. G. Zambonin, I. Losito, J.T. Triffitt, C.G. Zambonin
Detection of collagen synthesis by human osteoblasts on a tricalcium phosphate
hydroxyapatite. A X-Ray Photoelectron Spectroscopy (XPS) investigation
J. Biom. Mat. Res. 49 (2000) 120
13. C.G. Zambonin, I. Losito, F. Palmisano
Liquid chromatography/electrospray ionisation sequential mass spectrometric
identification of the main chlortoluron by-products during water disinfection using
chlorine
Rapid Comm. Mass Spectr. 14 (2000) 824
14. M. Quinto, I. Losito, F. Palmisano, C.G. Zambonin
A needle-type glucose microbiosensor based on an overoxidised polypyrrole film (an in
vitro study)
Fresenius J. Anal. Chem., 367 (2000) 692
15. M. Quinto, I. Losito, F. Palmisano, C.G. Zambonin
A disposable interference-free glucose biosensor based on an electropolymerised
poly(pyrrole) permselective film
Anal. Chim. Acta, 420 (2000) 9
16. I. Losito, C.G. Zambonin, F. Palmisano
Degradation of chlortoluron in water disinfection processes: a kinetic study
J. Environ. Monitoring, 2 (2000) 582
17. N. Cioffi, I. Losito, R. Terzano, C.G. Zambonin
An Electrospray Ionization-Ion Trap Mass Spectrometry (ESI-MS/MSn) study of
dehydroascorbic acid hydrolysis at neutral pH
Analyst 125 (2000) 2244
18. W. Lee, I. Losito, J.A. Gardella, W. L. Hicks
Synthesis and surface properties of fluorocarbons end-capped biodegradable
polyesters
Macromolecules 34 (2001) 3000
19. N. Cioffi, L. Torsi, I. Losito, C. Di Franco, I. De Bari, L. Chiavarone, G. Scamarcio, V.
Tsakova, L. Sabbatini, P.G. Zambonin
Electrosynthesis and analytical characterization of polypyrrole thin films modified
with copper nanoparticles
J. Mater. Chem. 11 (2001) 1434
20. I. Losito, E. De Giglio, N. Cioffi, C. Malitesta
Spectroscopic investigation on polymer films obtained by oxidation of ophenylenediamine on platinum electrodes at different pHs
J. Mater. Chem. 11 (2001) 1812
21. N. Cioffi, L. Torsi, I. Losito, L. Sabbatini, P.G. Zambonin, T. Bleve-Zacheo
3
Nanostructured palladium-polypyrrole composites electro-synthesised from organic
solvents
Electrochimica Acta 46 (2001) 4205
22. N. Cioffi, I. Losito, L. Torsi, I. Farella, A. Valentini, L. Sabbatini, P.G. Zambonin, T. BleveZacheo
Analysis of the surface chemical composition and morphological structure of vaporsensing gold-fluoropolymer nanocomposites
Chem. Mater. 14 (2002) 804
23. I. Losito, L. Monaci, A. Aresta, C.G. Zambonin
LC-ion trap electrospray MS-MS for the determination of cyclopiazonic acid in milk
samples
Analyst 127 (2002) 499
24. C.G. Zambonin, I. Losito, A. Cilenti, F. Palmisano
Solid-phase microextraction coupled to gas chromatography-mass spectrometry for
the study of soil adsorption coefficients of organophosphorous pesticides
J. Environ. Monit. 4 (2002) 477
25. E. De Giglio, I. Losito, L. Torsi, L. Sabbatini, P.G. Zambonin
Electroanalytical and spectroscopic characterization of poly(o-phenylenediamine)
grown on highly oriented pyrolytic graphite
Annali di Chimica 93 (2003) 209
26. I. Losito, N. Cioffi, M.P. Vitale, F. Palmisano
Characterization of soluble oligomers produced by electrochemical oxidation of ophenylenediamine by electrospray ionization sequential mass spectrometry
Rapid Commun. Mass Spectrom. 17 (2003) 1169
27. I. Losito, F. Palmisano, P.G. Zambonin
o-Phenylenediamine Electropolymerization by Cyclic Voltammetry Combined with
Electrospray Ionization-Ion Trap Mass Spectrometry
Anal. Chem. 75 (2003) 4988
28. E. De Giglio, I. Losito, F. Dagostino, L. Sabbatini, P.G. Zambonin, A. Torrisi, A. Licciardello
Analytical characterization of poly(pyrrole-3-carboxylic acid) films electrosynthesised on
Pt, Ti and Ti/Al/V substrates.
Annali di Chimica 94 (2004) 207
29. I. Losito, L. Monaci, F. Palmisano, G. Tantillo
Determination of ochratoxin A in meat products by high-performance liquid
chromatography coupled to electrospray ionisation sequential mass spectrometry.
Rapid Commun. Mass Spectrom. 18 (2004) 1965
30. I. Losito, A. Amorisco, F. Palmisano, P.G. Zambonin
X-ray
photoelectron
spectroscopy
characterization
of
composite
TiO2–
poly(vinylidenefluoride) films synthesised for applications in pesticide photocatalytic
degradation.
Applied Surface Science 240 (2005) 180
4
31. I. Losito, C. Malitesta, I. De Bari, C. D. Calvano
X-ray photoelectron spectroscopy characterization of poly(2,3-diaminophenazine) films
electrosynthesised on platinum
Thin Solid Films 473 (2005) 104
32. E. De Giglio, C.D. Calvano, I. Losito, L. Sabbatini, P.G. Zambonin A. Torrisi, A. Licciardello
Surface (XPS, SIMS) chemical investigation on poly(pyrrole-3-acetic acid) films
electrosynthesised on Ti and TiAlV substrates for the development of new bioactive
substrates.
Surf. Interf. Anal. 37 (2005) 580
33. A. Amorisco, I. Losito, F. Palmisano, P.G. Zambonin
Photocatalytic degradation of the herbicide isoproturon: characterisation of by-products
by liquid chromatography with electrospray ionisation tandem mass spectrometry
Rapid Commun. Mass Spectrom. 19 (2005) 1507
34. C.G. Rizzello, I. Losito, M. Gobbetti, T. Carbonara, M.D. De Bari, P.G. Zambonin
Isolation and Characterization of Antimicrobial Peptides from Several Italian Cheese
Varieties.
Journal of Dairy Science 88 (2005) 2348
35. S. Pati, I. Losito, F. Palmisano, P.G. Zambonin
Characterization of caffeic acid enzymatic oxidation by-products by
chromatography coupled to electrospray ionization tandem mass spectrometry
J. Chromatog. A 1102 (2006) 184
liquid
36. M. De Angelis, C.G. Rizzello, A. Fasano, M.G. Clemente, C. De Simone, M. Silano, M. De
Vincenzi, I. Losito, M. Gobbetti
VSL#3 probiotic preparation has the capacity to hydrolyze gliadin polypeptides
responsible for Celiac Sprue
Biochimica et Biophysica Acta, Molecular Basis of Disease 1762 (2006) 80
37. I. Losito, T. Carbonara, M.D. De Bari, M. Gobbetti, F. Palmisano, C.G. Rizzello, P.G.
Zambonin
Identification of peptides in antimicrobial fractions of cheese extracts by electrospray
ionization ion trap mass spectrometry coupled to a two-dimensional liquid
chromatographic separation.
Rapid Communications in Mass Spectrometry 20 (2006) 447
38. A. Amorisco, I. Losito, T. Carbonara, F. Palmisano, P.G. Zambonin
Photocatalytic degradation of phenyl-urea herbicides chlortoluron and chloroxuron:
characterization of the by-products by liquid chromatography coupled to electrospray
ionization tandem mass spectrometry.
Rapid Communications in Mass Spectrometry 20 (2006) 1569
39. S. Pati, I. Losito, G. Gambacorta, E. La Notte, F. Palmisano, P.G. Zambonin
Simultaneous separation and identification of oligomeric procyanidins and anthocyaninderived pigments in raw red wine by HPLC-UV-ESI-MSn
J. Mass Spectrom. 41 (2006) 861
40. I. Losito, A. Amorisco, T. Carbonara, S. Lofiego, F. Palmisano
5
Simultaneous determination of phenyl- and sulfonyl-urea herbicides in river water at subparts-per-billion level by on-line preconcentration and liquid chromatography–tandem
mass spectrometry
Anal. Chim. Acta 575 (2006) 89
41. C.G. Rizzello, M. De Angelis, R. Di Cagno, A. Camarca, M. Silano, I. Losito, M. De Vincenzi,
M.D. De Bari, F. Palmisano, F. Maurano, C. Gianfrani, M. Gobbetti
Highly efficient gluten degradation by lactobacilli and fungal proteases during food
processing: new perspectives for celiac disease
Appl. Envir. Microbiol. 73 (2007) 4499
42. I. Losito, T. Carbonara, L. Monaci, F. Palmisano
Evaluation of the thermal history of bovine milk from the lactosylation of whey proteins:
an investigation by liquid chromatography–electrospray ionization mass spectrometry
Anal. Bional. Chem. 389 (2007) 2065
43. I. Losito, A. Amorisco, F. Palmisano
Electro-Fenton and photocatalytic oxidation of phenyl-urea herbicides: an insight by
liquid chromatography–electrospray ionization tandem mass spectrometry
Applied Catalysis B: Environmental 79 (2008) 224
44. I. Losito, R. Angelico, A. Ceglie, S. Diomede, F. Palmisano
Alkylation of complementary ribonucleotides by 1,2-dodecyl-epoxide in a micellar
environment: a liquid chromatography-electrospray ionization-sequential mass
spectrometry investigation
J. Mass Spectrom. 44 (2009) 1053
45. I. Losito, E. Stringano, S. Carulli, F. Palmisano
Correlation between lactosylation and denaturation of major whey proteins: an
investigation by liquid chromatography-electrospray ionization mass spectrometry
Anal. Bioanal. Chem. 396 (2010) 2293
46. R. Angelico, L. Ambrosone, A. Ceglie, I. Losito, G. De Zio, F. Palmisano
Complementary amphiphilic ribonucleotides confined into nanostructured environments
Phys. Chem. Chem. Phys. 12 (2010) 7977-7987
47. L. Monaci, I. Losito, F. Palmisano, A. Visconti
Identification of allergenic milk proteins markers in fined white wines by capillary liquid
chromatography–electrospray ionization-tandem mass spectrometry
J. Chromatogr. A 1217 (2010) 4300-4305
48. T.R.I. Cataldi, G. Bianco, I. Losito, S. Carulli
A validated interpretation of the collision-induced dissociation of protonated 5’methylthioadenosine through selected A+1 and A+2 isotope fragmentations by tandem
mass spectrometry
Rapid Commun. Mass Spectrom. 24 (2010) 2925–2930
49. L. Monaci, I. Losito, F. Palmisano, A. Visconti
Reliable Detection of Milk Allergens in Food Using a High-Resolution, Stand-Alone Mass
Spectrometer
J. of AOAC Int. 94 (2011) 1034-1042
6
50. T.R. I. Cataldi, G. Bianco, S. Abate, I. Losito
Identification of unsaturated N-acylhomoserine lactones in bacterial isolates of
Rhodobacter sphaeroides by liquid chromatography coupled to electrospray ionizationhybrid linear ion trap-Fourier transform ion cyclotron resonance mass spectrometry
Rapid Comm. Mass Spectrom. 25 (2011) 1817-1826
51. L. Monaci, I. Losito, F. Palmisano, M. Godula, A. Visconti
Towards the quantification or residual milk allergens in caseinate-fined white wines using
HPLC coupled with single-stage Orbitrap Mass Spectrometry
Food Additives and Contaminants: Part A 28 (2011) 1304-1314
52. I. Losito, E. Conte, B. Introna, F. M. Megli, F. Palmisano
Improved specificity of cardiolipin peroxidation by soybean lipoxygenase: a liquid
chromatography - electrospray ionization mass
spectrometry investigation
J. Mass Spectrom. 46 (2011) 1255-1262
53. I. Losito, F. Mavelli, A. Demarinis Loiotile, F. Palmisano
A support for the identification of non-tryptic peptides based on low resolution tandem
and sequential mass spectrometry data: The INSPIRE software
Anal. Chim. Acta 718 (2012) 70– 77
54. I. Losito, R. Angelico, B. Introna, A. Ceglie, F. Palmisano
Cytosine to uracil conversion through hydrolytic deamination of cytidine
monophosphate hydroxy-alkylated on the amino group: a liquid chromatographyelectrospray ionization-mass spectrometry investigation
J. Mass Spectrom. 47 (2012) 1384-1393
55. R. Angelico, I. Losito, F. Cuomo, A. Ceglie, F. Palmisano
Alkylation of Complementary Ribonucleotides in Nanoreactors
Phys. Chem. Chem. Phys. 15 (2013) 585-595
56. R. Zianni, G. Bianco, F. Lerario, I. Losito, F. Palmisano, T.R.I. Cataldi
Fatty acid neutral losses observed in tandem mass spectrometry with collisioninduced dissociation allows regiochemical assignment of sulfoquinovosyldiacylglycerols
J. Mass Spectrom. 48 (2013) 205-215
Brevetti
J.A. Gardella Jr., W. Hicks Jr., T. Koloski, I. Losito, W. Lee, T.G. Vargo
Biodegradable fluorocarbon end-capped polyesters and method of synthesis.
WO 2001023454/ US 6329470
Libri
7
I. Losito, L. Sabbatini, J.A. Gardella Jr.
Electron, ion, and mass spectrometry in Comprehensive Desk Reference of Polymer
Characterization and Analysis, editore: R. F. Brady Jr., Oxford University Press, 2003, pag.
375-407.
8