Relazione ScientificaAprile2010

Transcript

Relazione ScientificaAprile2010
Università di Trieste
Scuola di dottorato di ricerca in Nanotecnologie
Resoconto dell’attività 2009
Sommario
1.
2.
3.
Relazione scientifica riassuntiva .................................................................................................. 3
Elenco dottorandi, supervisori e titolo delle tesi .......................................................................... 5
Schede attività dottorandi............................................................................................................. 7
Dottorandi del 22 ciclo:.................................................................................................................... 8
Dottorandi del 23 ciclo:.................................................................................................................. 33
Dottorandi del 24 ciclo: • ............................................................................................................... 53
Research activity ............................................................................................................................ 58
Research activity ............................................................................................................................ 83
4. Progetti Dottorandi del 25 ciclo: ................................................................................................ 89
5. Pubblicazioni dei dottorandi. ................................................................................................... 119
6. Giudizi della commissione d‟esame finale 2009 ..................................................................... 127
7. Elenco dei componenti del collegio docenti ............................................................................ 130
8. Pubblicazioni rappresentative del collegio docenti .................................................................. 133
9. Criteri di valuatazione delle scuole di dottorato ...................................................................... 205
10.
Valutazione da parte del nucleo di valutazione dell‟Ateneo ................................................. 209
11.
Giudizi dei referee esterni per dottorandi ammessi all‟esame finale .................................... 210
12.
Presentazioni dei dottorandi al congesso del 2009 ............................................................... 211
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Attività della scuola: situazione ad aprile 2010.
Questo documento contiene il dettaglio dell‟attività didattico scientifica della scuola di dottorato in
nanotecnologie svolto durante l‟anno 2009 fino ad aprile del 2010. Il documento si compone delle
seguenti parti:
1. Relazione scientifica riassuntiva (Executive summary)
2. Elenco dei dottorandi, supervisori e titolo tesi in corso.
3. Schede di attività dei dottorandi che hanno svolto attività continuativa nell‟anno 2009.
Questa attività è stata presentata nel congresso di gennaio 2010 (vedi slides su CD allegato)
ed è riassunto per ogni dottorando nelle seguenti schede. Le schede si riferiscono ai
dottorandi in corso dei cicli 22, 23 e 24, non essendo nesssun dottorando del ciclo 21 in
proroga.
4. Progetti dei dottorandi nuovi entrati nella scuola (25 ciclo). Questa attività non è stata
presentata in gennaio 2010 poiché non ancora definiti gli accoppiamenti dottorando – tema
di ricerca.
5. Riassuntivo delle pubblicazioni dei dottorandi..
6. Giudizi della commissione d‟esame finale 2010.
7. Elenco dei componenti collegio docenti.
8. Pubblicazioni rappresentative del collegio docenti.
9. Criteri di valutazione delle scuole di dottorato.
10. Valutazione della scuola da parte del nucleo di valutazione riassunta in una tabella e rimandi
ad (allegati).
11. Giudizi dei reviewer esterni per i dottorandi ammessi all‟esame finale (allegati).
12. Presentazioni dei dottorandi al congresso del 2010 (allegati).
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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1. Relazione scientifica riassuntiva
La missione della scuola di dottorato è di migliorare le conoscenze ed educare studenti nel campo
delle nanotecnologie al fine di formare scienziati e tecnici per il 21° secolo. La caratteristica della
Scuola è l‟interdisciplinarità: sugli argomenti di ricerca attivi lavorano in sinergia fisici, chimici,
biologi, ingegneri, medici, farmacologi, odontoiatri, biotecnologi e laureati in Agraria, mantenendo
e rafforzando la specificità della cultura di provenienza ed acquisendo la capacità di sviluppare la
propria ricerca in un quadro più ampio.
L‟obiettivo principale della Scuola è di formare Ricercatori che sappiano progettare, costruire,
utilizzare e sottoporre a prove di funzionalità strumenti e dispositivi nano tecnologici, in grado di
rispondere alle crescenti e diversificate esigenze delle applicazioni. L'allievo “dottorato” di questa
Scuola sarà un professionista della ricerca e dello sviluppo tecnologico che sappia applicare le
proprie conoscenze, con capacità di valutazione critica, allo sviluppo di metodi di progettazione,
produzione e valutazione di nuovi materiali e al miglioramento di quelli esistenti. Questo anche
mirato ad una produzione industriale più efficace, economica e sostenibile dal punto di vista delle
risorse e dell'ambiente. La scuola è stata fondata nel 2006 a partire da un dottorato in
nanotecnologie.
Gli obiettivi delle ricerche sono i seguenti:
Sviluppo di nuove tecniche sperimentali per lo studio, la lavorazione, la manipolazione e la
visualizzazione su scala nanometrica di materiali nanostrutturati.
Sviluppo di tecniche spettroscopiche di rivelazione di singola molecola su substrati
nanostrutturati.
Studio delle relazioni tra la microstruttura e le proprietà dei materiali e ingegnerizzazione di
materiali nanostrutturati.
Sintesi di nanostrutture.
Applicazioni delle nanotecniche e nanostrutture a ricerche di interesse biomedico ed
energetico.
Modellizzazione molecolare multiscala di materiali e di fenomeni di interesse attraverso
tecniche di simulazione computazionale.
Salute umana con particolare attenzione allo studio ed al trattamento di tumori e malattie
degenerative.
Applicazione delle nanotecnologie nei settori medico, farmacologico e biomedico
Questi obiettivi sono perseguiti avvalendosi delle attrezzature d'avanguardia disponibili nei
laboratori dell'Università di Trieste e degli Enti di ricerca pubblici e privati convenzionati con
l‟Università di Trieste: il centro internazionale di ingegneria genetica e Biotecnologie (ICGEB), il
Sincrotrone TS, il CRO di Aviano ed il laboratorio nazionale TASC- INFM- CNR.
Di seguito alcuni numeri rappresentativi dell‟attività della scuola:
 n. di studenti (ad aprile 2010): 32
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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 Numero di diplomati nel 2010: 7
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Numero di diplomati nel 2009: 6
Numero di diplomati nel 2008: 9
Numero di diplomati nel 2007: 14
Membri del collegio docenti: 48
Enti di ricerca coinvolti nel dottorato:
o Dipartimenti dell‟Università di Trieste: 10
o Enti di ricerca esterni: 5
o Altre Università: 5
 Numero di pubblicazioni degli studenti (anno 2009): 83 (comprese di brevetti e
presentazioni a convegni) di cui 5 brevetti e 47 pubblicazioni su riviste internazionali
 Numero di pubblicazioni di supervisori e tutors (2004-09): 1002
 Risorse finanziarie (per anno): 520.000 euro
 Ultima valutazione complessiva del nucleo dell‟Università di Trieste: A+ (Ottimo)
I criteri usati per la valutazione interna sono stati quelli suggeriti dal Ministero, accompagnati da
criteri interni all‟Ateneo. Questi criteri sono descritti di seguito in questo documento in modo
dettagliato. I principali indicatori utilizzati dal nucleo che hanno permesso la valutazione in classe
A+ sono:
 produzione scientifica del Collegio Docenti;
 congruo numero di docenti dell‟Ateneo coinvolti nel collegio;
 rapporto del dottorato con il mondo del lavoro;
 attrattività del dottorato nelle precedenti edizioni;
 livello di soddisfazione dei dottorandi;
 produzione scientifica dei dottorandi ed iniziative esistenti di pubblicizzazione e referaggio
delle tesi di dottorato;
 adeguata formulazione delle tematiche scientifiche e degli obiettivi formativi,
 riduzione della frammentazione dei corsi di dottorato;
 accuratezza delle informazioni fornite per la valutazione.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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2.Elenco dottorandi, supervisori e titolo delle tesi
Dottorando Ciclo Supervisore
FRAGKOU
Effrosyni
GANAU Mario
25 STANTA
Giorgio
25 SCOLES
Giacinto
GIORGIS
Valentina
AMBROSINI
Stefano
CHEN Yan Xin
25 ROMANATO
Filippo
25 RUBINI Silvia
SAMMITO
Davide
LUCAFO'
Marianna
PERONIO
Angelo
MARSICH Lucia
BIDOGGIA
Silvia
CARGNELLO
Matteo
D'ESTE Elisa
ERA Daniel
ELIA Caterina
PAKDAST
Hossein
SCIUTO
Giacomo
COZZARINI
Luca
ZANUSSO
Chiara
TAVANO
Federica
25 FORNASIERO
Paolo
25 ROMANATO
Filippo
25 SAVA Gianni
25 COMELLI
Giovanni
25 SERGO Valter
SSD
Titolo Tesi
MED/08 Nanobiotechnologies for Dermatological Applications
FIS/03
Nanotechnology applications in quantitative
neuroscience: proteomics assessment of astrocyte
stressing and neuronal plasticity
FIS/03
Development, design and characterization of
Metamaterials with negative refractive index
FIS/01
Sintesi di nanostrutture di semiconduttori e loro studio
mediante luce di sincrotrone.
CHIM/03 Nanostructured Titanium Dioxide Based Materials for
Photocatalysis and Phototelectrocatalysis
FIS/03
Design, Fabrication and Characterization of Plasmonic
Nanostructures for Solar Cells Light Harvesting
Enhancement.
BIO/14 Study of carbon nanostructures as carriers for drugs for
cancer chemotherapy.
FIS/03
Single-molecule heterogeneous catalysis
INGIND/22
Design and synthesis of functionalized metal
nanoparticles for bio-analysis with Surface-Enhanced
Raman Scattering (SERS)
25 PASQUATO
CHIM/06 Gold nanoparticles as carriers for drug delivery
Lucia
applications
24 FORNASIERO CHIM/03 Rare earths oxides based nanostructures as
Paolo
heterogeneous catalysts
24 MORGANTE
FIS/01
Bio-sample environment manipulation using advanced
Alberto
microscopy techniques
24 GIRALDI Tullio BIO/14 Nanotechnology application in pharmacogenetic research
24 TOFFOLI
BIO/14 Molecular Interaction on Nanoscale in Cancer Therapy
Giuseppe
24 LAZZARINO
FIS/01
Single Molecule Detection with Advanced MEMS
Marco
Resonators
24 MUSCIA
INGInnovative LHTS Elements Based on Nanotechnologies
Roberto
IND/15
24 SERGO Valter INGQuantum dot-based materials
IND/16
24 TOFFOLI
BIO/14 Nanotechnologies and oncology:
Giuseppe
pharmacokinetics and pharmacogenomics to optimize the
antitumor therapie
24 BONIN Serena MED/35 Cell mobility and metastatic spreading: a study on human
neoplastic cells using
optical tweezer
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Dottorando Ciclo Supervisore
SSD
FIOR Raffaella
24 SBAIZERO
Orfeo
INGIND/22
LANZILOTTO
Valeria
LUISI
Immacolata
MIGLIORINI
Elisa
24 MORGANTE
Alberto
24 MARZARI
Roberto
24 LAZZARINO
Marco
FIS/01
MARINI
Monica
ASTOLFO
Alberto
SANTARELLI
Xenja
BELLOMO
Francesca
RAMPINO
Antonio
SOVERNIGO
Enrico
MARCHESI
Giulio
BIRARDA
Giovanni
24 FIRRAO
AGR/12
Giuseppe
23 ARFELLI Fulvia FIS/07
FONTANIVE
Luca
23 ROSEI Renzo
BIO/06
FIS/01
FIS/03
Titolo Tesi
Ionic channel behavior: development of a novel, non
destructive method for mechanical and electrical analysis
of a single cell
Self assembly and charge transfer properties of thin
organic films at metal surfacese
Identification of novel protein scaffolds for binding of
small molecules
Characterization of nanostructured substrates which
control the Embryonic Stem Cells differentiation into
neurons
Implementation of simple logics in bioinorganic hybrid
nanosensors
Development of novel experimental methods for cell
tracking using nanoparticles
Correlation between structure and reactivity for
nanostructured catalysts
Biopolymer based scaffold for tissue engineering
23 PAOLETTI
BIO/10
Sergio
23 CESARO Attilio CHIM/04 Polysaccharide based nanoparticles for drug delivery
23 MORGANTE
Alberto
23 BRESCHI
Lorenzo
23 FRANCIOSI
Alfonso
FIS/01
Influence of nanostructure on the electronic properties
of heterojunctions in photovoltaic cells
MED/28 Analysis of aging and stability of bonded interface in
dentistry
FIS/01
Development of microfluidic devices for biomedical
applications of Synchrotron Radiation FTIR
Microspectroscopy
23 CESARO Attilio CHIM/04 Nanoscale interaction for higher efficiency of contrast
media
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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3.Schede attività dottorandi
Questo allegato contiene le schede dei dottorandi della scuola di nanotecnologia così come sono
pervenute al 31 gennaio 2009. Sono quindi incluse le schede dei dottorandi dei cicli 22, 23 e 24.
 Dottorandi del 22 ciclo: DAL MIGLIO Matteo Maria, LACOVIG Paolo, LORENZUT
Barbara, PAVAN Giovanni Maria, POSOCCO Paola, TRAVAN Andrea, UMERI Andrea.
 Dottorandi del 23 ciclo: ASTOLFO Alberto, BELLOMO Francesca, BIRARDA Giovanni,
FONTANIVE Luca, MARCHESI Giulio, RAMPINO Antonio, SANTARELLI Xenja,
SOVERNIGO Enrico
 Dottorandi del 24 ciclo: CARGNELLO Matteo, COZZARINI Luca, D‟ESTE Elisa, ELIA
Caterina, ERA Daniel, FIOR Raffaella, LANZILOTTO Valeria, LUISI Immacolata,
MARINI Monica, MIGLIORINI Elisa, PAKDAST Hossein, SCIUTO Giacomo, TAVANO
Federica, ZANUSSO Chiara.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Dottorandi del 22 ciclo:
Candidate Matteo Maria Dalmiglio
Title of the thesis: Reactivity and oxidation properties of catalytic supported nanoclusters
Supervisor: Luca Gregoratti
Tutors (if any):
Research Activity
The research activities performed in this 3 years were focused on studying the reactivity and
oxidation properties of catalytic supported nanocluster on oxide films and carbon nanotubes
(CNTs), by means of different chemical and structural characterization techniques; in particular the
main technique used was the scanning photoemission spectromicroscopy (SPEM) available at the
EscaMicroscopy beamline of the Elettra Synchrotron Light Source. This novel experimental
technique allows sample‟s chemical mapping with a spatial resolution of 100nm and the acquisition
of photoemission spectra on regions with the same dimension. In particular we studied PtRh cluster
deposited by PLD (pulsed laser deposition) on a tungsten single crystal (W(110)) covered by a thin
magnesium oxide film (MgO).
The starting of the research activities related to this project was preceded by a training aimed to
become familiar with the experimental setup and data acquisition procedures. During this time I
participated to several user beamtime. The first action strictly related to my thesis was focused on
the realization of a setup for the coverage of a micron-sized region of a single MCNT by using
commercial and home-made masks. This setup was used to produce nanoclusters covered patches as
model systems to investigate surface and bulk diffusion, mass transport, etc. The cluster‟s
characterization and study was performed with AFM (atomic force microscope) and SEM (scanning
electron microscope) in collaboration with other laboratory at Elettra and TASC.
Afterwards different experiments were made with the aim to understand the nanoclusters catalytic
activity during oxidation and reduction conditions. It was demonstrated that under an atomic
oxygen flux a selected oxidation occurs: rhodium atoms undergo stronger and faster oxidation than
platinum ones. Furthermore, the oxidation process is composed by many intermediate steps, in
which metastable oxides are formed. Very small cluster‟s oxidation (<10nm diameter) is
significantly faster then the bigger one (>100nm). Some morphological and structural clusters‟
modifications after long oxidation treatments were also investigated using a high resolution SEM
(<2nm lateral resolution).
In this last third year the nanocluster reactivity towards redox reactions after many reaction cycles
has been investigated in detail; the chemical characterizations have been carried out on the as
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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deposited particles and on the same particles after many reactions cycles in order to probe the
modifications of the catalysts. The results obtained confirm some previous evidences on the
decreasing oxides‟ stability. In fact after the same oxidation rate the “aged” particles are easily
reduced; sometimes the photon beam intensity was enough to reduce the samples in a spectra
acquisition time.
Some other measurements were performed at the Nanospectroscopy beamline where it‟s possible to
use a LEEM microscope ((Low Energy Electron Microscope) that combines a high spatial
resolution (<5nm) to a high sensitivity to surface structural modifications. In particular the
behaviour of the clusters‟ polycrystalline structure was studied during oxidation-reduction
treatments. It was shown that the clusters‟ surface is polycrystalline: the nano crystals on the surface
had different orientation from region to region of the sample and undergo different oxidation rate.
The diffraction pattern revealed that after a long oxidation all the informations on the long range
order are completely lost. Other experiments were realized with SEM and AFM for studying the
clusters‟ morphology and behaviour at different annealing temperature. The results showed
structural and surface morphology changes on the clusters‟ facets.
Along this year a peer review paper that summarizes the main results obtained during this PhD has
been submitted to the Journal of Physical Chemistry .
Other time was dedicated to prepare posters and presentations for conferences and a summer school
on reactivity of nanoparticles in different European countries.
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Publications on scientific journals (printed or in press)
Barinov, A., Malcioǧlu, O.B., Fabris, S., Sun, T., Gregoratti, L., Dalmiglio, M., Kiskinova,
M. Initial stages of oxidation on graphitic surfaces: Photoemission study and density
functional theory calculations, 2009, Journal of Physical Chemistry C 113 (21), pp. 90099013
Bozzini, B., Dalmiglio, M., De Gaudenzi, G.P., D'Urzo, L., Gregoratti, L. An investigation
of the corrosion of WC-Co cermets in CN--containing aqueous solutions. Part II:
Synchrotron-based high lateral-resolution XPS study, 2009, Corrosion Science 51 (8), pp.
1675-1678
M. Dalmiglio, M. Amati, L. Gregoratti, T. O. Menteş, M. A. Niño, L. Felisari and M.
Kiskinova, Catalytic activity of PtRh particles, submitted to Journal of Physical Chemistry
Publications/abstracts in conferences/congresses (national or international)
Books /Chapters and sections of books
Other publications
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Participation to conferences (as speaker)
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First common workshop of the 'Scuola di Dottorato di Ricerca in Nanotecnologie' and the
'Nano-IMPRS' in Halle on July 15-16, 2009.
Educational Activity
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Periods abroad (date and place)
14-17/07/2009: Halle, Max Plank Institute
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21-29/08/2009: Sonderborg (DK), Summer School on 'Reactivity of nanoparticles for more
efficient and sustainable energy production'
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09-11/09/2009: Wittenberg, Joint Workshop of SFB762 & IMPRS
Classes followed (date, course, professor, type of course)
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Conferences, seminars, advanced courses and other didactic activities
Congresso scuola di dottorato di gennaio
X-ray microscopy: dealing with real-life complexities
Modelling reactions at the nanoparticle scale, Prof. Phillip R. Westmoreland Dept of
Chemical Engineering, University of Massachusetts Amherst
Low-dimensional oxide structures on metal surfaces
Stress-induced Mesoscopic Patterns on a Single Crystal Surface, Tevfik Mentes
Chimica fisica delle interfasi in sistemi nano strutturati, Dr Italo Colombo
Summer School on 'Reactivity of nanoparticles for more efficient and sustainable
energy production' in Sonderborg (DK)
XPS Measurements for Probing Dynamics of Chargin
How to write (and manage) a research project
Support educational activity and teaching
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Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Candidate PAOLO LACOVIG
Title of the thesis: Electronic structure, morphology and chemical reactivity of nanoclusters and
low-dimensional systems: fast photoemission spectroscopy studies
Supervisor: Dr. Alessandro BARALDI (Physics Department – Uni TS)
Tutors (if any): Dr. Silvano LIZZIT (Sincrotrone Trieste S.C.p.A.)
Research Activity
During the three-year PhD research project Paolo Lacovig was involved in the development of
innovatory instrumentation for high energy- and high temporal-resolution acquisitions of
photoemission spectra and its applications. From the latter point of view the experimental activity
hinged on the study of several physical systems for which the characteristics of the new
experimental set-up have been fully exploited.
The first year activity was focused on the hardware and software development for the new electron
energy analyser installed on the SuperESCA beamline at the Elettra synchrotron light source. The
analyser commissioning phase focused on the estimation of the energy (25 meV) and temporal
resolution (80 ms) achievable with the new instrumentation in synchrotron radiation photoemission
experiments. The innovative properties of this novel instrument have been exploited for
investigations on the electronic structure, morphology and chemical properties of low-dimensional
systems.
One of the most significant results concerns the understanding of the modifications in the chemical
reactivity of bimetallic interfaces due to the different thickness of the pseudomorphically grown
overlayer. In the case of Pd-Ru bimetallic interface, we found out how the surface electronic
structure, and its chemical properties as a consequence, can be tuned by varying the number of Pd
atomic layers grown on the Ru(0001) surface.
The temporal resolution achieved with the new electron energy analyser allowed studies on
dynamical processes up to now inaccessible; in particular the investigation of the the graphene
growth process at high temperature on transition metal surfaces (Ir, Pt and Rh) and the study of the
chemical reactivity of Pt nanoclusters supported on MgO. In the first case, we discovered that
graphene formation proceeds via preliminary nucleation of dome-shaped C nano-islands. In the
second case, we succeded in following both the dynamics of CO adsorption process and the
CO+O2→CO2 reaction on Pt nano-clusters grown on a ultra-thin film of magnesium oxide.
Finally, the morphology of Pd, Pt, Rh and Au nanoclusers grown on different carbon-based
substrates (namely graphite, single-walled carbon nanotubes and graphene) has been characterized.
Among the most interesting results we report the understanding of the dependence of the metalsubstrate interaction on the cluster size and the role of defects in the nucleation and intercalation
processes.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Publications on scientific journals (printed or in press)
1. G. Cautero, R. Sergo, L. Stebel, P. Lacovig, P. Pittana, M. Predonzani, S. Carrato, A twodimensional detector for pump-and-probe and time resolved experiments, Nucl. Instrum.
Methods A 595, 447-459 (2008)
2. P. Lacovig, M. Pozzo, D. Alfè, P. Vilmercati, A. Baraldi, S. Lizzit, Growth of Dome-Shaped
Carbon Nanoislands on Ir(111): The Intermediate between Carbidic Clusters and QuasiFree-Standing Graphene, Phys. Rev. Lett. 103, 166101 (2009)
3. E. Golfetto, A. Baraldi, M. Pozzo, D. Alfè, A. Sala, P. Lacovig, E. Vesselli, S. Lizzit, G.
Comelli, R. Rosei, Determining the chemical reactivity trends of Pd/Ru(0001)
pseudomorphic overlayers: core level shift measurements and DFT calculations, J. Phys.
Chem. C, in press DOI: 10.1021/jp908568v
Other publications
1. G. Cautero, R. Sergo, L. Stebel, P. Lacovig, P. Pittana, M. Predonzani, S. Carrato, “A twodimensional detector for pump-and-probe and time resolved experiments”,
Highlights 2007-2008, 110 (2008)
Elettra
Participation to conferences (as speaker)
1. POSTER - P. Lacovig, M. Pozzo, D. Alfè, P. Vilmercati, A. Baraldi, and S. Lizzit,
Graphene Growth on Ir(111): A Fast XPS Study - CINF-DTU Summer School on
“Reactivity of nanoparticles for more efficient and sustainable energy production”,
Sandbjerg Gods (Denmark), 2009
2. POSTER – P. Lacovig, A. Baraldi, S. Lizzit, Chemical reactivity of nanoclusters deposited
on solid surfaces: new time-domain and enhanced surface-sensitivity of photoelectron
spectroscopy - Joint Workshop of SFB762 & IMPRS on “Magnetic properties of oxidic and
multiferroic systems”, Wittenberg (Germany), 2009
Educational Activity
Classes followed (date, course, professor, type of course)
 COURSE: “Advanced microscopy”, April-June, 2007 (C. Africh, V. Grillo) - 20 hours
 COURSE: “Photoemission Spectroscopy and Spectro-Microscopy”, February-March,
2008 (A. Baraldi, A. Goldoni, A. Locatelli) - 25 hours
 REVIEW ARTICLE: B. Hammer, J.K. Norskov, “Theoretical Surface Science and
Catalysis – Calculation and Concepts”, Advances in Catalysis 45, (2000) 71-128
 BOOK CHAPTER: C.S. Fadley, “Basic Concepts of X-Ray Photoelectron Spectroscopy”
in C.R. Brundle, A.D. Baker (Eds.), “Electron Spectroscopy: Theory, Techniques and
Applications”, Pergamon Press, New York, 1978, 1-156
 BOOK: M. Cardona, L. Ley (Eds.), “Photoemission in Solids I: General Principles”,
Springer-Verlag, Berlin, 1978, 1-288
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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REVIEW ARTICLE: D.P. Woodruff, A.M. Bradshaw, “Adsorbate structure
determination of surfaces using photoelectron diffraction”, Rep. Prog. Phys. 57 (1994)
1029-1080
REVIEW ARTICLE: J. Libuda, H.-J. Freund, “Molecular beam experiments on model
catalysts”, Surf. Sci. Rep. 57 (2005) 157-298
REVIEW ARTICLE: C.T. Campbell, “Ultrathin metal films and particles on oxide
surfaces: structural, electronic and chemisorptive properties”, Surf. Sci. Rep. 27 (1997) 1111
REVIEW ARTICLE: M. Baumer, H.-J. Freund, “Metal deposits on well-ordered oxide
films”, Progr. Surf. Sci. 61 (1999) 127-198
LABORATORY: “Operation of the SuperESCA beamline: alignment and calibration
procedure; introduction to the main beamline instrumentation”, February, 2007 (S. Lizzit,
S. Gardonio) – 16 hours
LABORATORY: “Sample mounting techniques and cleaning procedures for transition
metal single crystals”, April, 2007 (S. Lizzit) – 12 hours
LABORATORY: “Growth of oxide layers on transition metal”, May, 2007 (A. Baraldi) –
10 hours
LABORATORY: “Evaporator assemblies and chemical vapor deposition of transition
metal nanoclusters ”, June, 2007 (S. Lizzit) – 16 hours
LABORATORY: “Data analysis techniques”, October, 2007 (S. Lizzit, A. Baraldi, S.
Gardonio) – 12 hours
Conferences, seminars, advanced courses and other didactic activities
CONFERENCE - Nanotech Conference 2008 – Trieste – January 15th-17th, 2008
CONFERENCE - Nanotech Conference 2009 – Trieste – January 13th-15th, 2009
CONFERENCE - Joint Workshop SFB762 & IMPRS on “Magnetic properties of oxidic
and multiferroic systems” – Wittenberg, Germany – September 10th-11th, 2009
SEMINAR – Confined and composite soft matter on the nanoscale (M.L. Saboungi) –
Trieste – April 11th, 2008
SEMINAR – Modeling reactions at the nanoparticle scale (P.R. Westmoreland) – Trieste
– May 8th, 2009
SEMINAR – Chimica fisica delle interfasi in sistemi nanostrutturati (I. Colombo) –
Trieste – May 15th, 2009
SEMINAR – How to write (and manage) a research project (C. Blasetti, V. Sergo, M.
Fermeglia) – October 16th, 2009
MEETING – Collegamento VPN e nuovo portale della Scuola di Dottorato (M.
Fermeglia) – Trieste – March 20th, 2009
MEETING – Panoramica delle nuove modalità di comunicazione scientifica (S. Arabito,
A. Bonazza, J. Piščanc) – Trieste – June 30th, 2009
SCHOOL – ARS 2 – Synchrotron radiation applications for nanostructured material and
thin film investigations – Trieste – April 23rd-26th, 2007
SCHOOL – Summer School 2009 - Reactivity of nanoparticles for more efficient and
sustainable energy production (CINF, DTU) – Sandbjerg Gods, Denmark – August 22nd27th, 2009
WORKSHOP – Bio Meets Nano: a surface scientist approach to bio-organic interfaces –
Trieste – March 16th, 2007
SEMINAR – Nanoscale Fermi systems: Structure and dynamics of metal clusters (B. von
Issendorf) – Trieste – March 7th, 2007
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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SEMINAR – Reaction kinetics on nanostructured materials (J. Libuda) – Trieste – March
26th, 2007
SEMINAR – Ultrathin ordered titanium oxide films on Pt(111) properties and
perspectives (F. Sedona) – Trieste – March 28th, 2007
SEMINAR – Quasi 1-D Metal Oxide and Phthalocyanine Nanostructures as Gas Sensors:
Fabrication, Characterization and Prototype Devices (A. Kolmakov) – Trieste – June
20th, 2007
SEMINAR – Time resolved photoemission on Mott insulators and High Temperature
Superconductors (L. Perfetti) – Trieste – July 17th, 2007
SEMINAR – X-ray micro- and nano-spectroscopies at ESRF (M. Salome) – Trieste –
September 26th, 2007
SEMINAR – Electron-phonon interaction in graphene: non-adiabatic Kohn anomalies
and electron-mass enhancement (F. Mauri) – Trieste – October 25th, 2007
SEMINAR – XAS measurements under extreme conditions at third generation
synchrotron sources (S. De Panfilis) – Trieste – December 6th, 2007
SEMINAR – X-ray absorption spectroscopy and high pressure science at ESRF:
techniques, results, and future opportunities (G. Aquilanti) – Trieste – December 6th,
2007
SEMINAR – Studies of carbon nanotubes and nano-crystalline Silicon under high
pressures (S.M. Sharma) – Trieste – December 14th, 2007
SEMINAR – The power of SAXS: exploration of the magic world of biomaterials (M.
Rappolt) – Trieste – February 1st, 2008
SEMINAR – Electron-Phonon Superconductivity in graphite (L. Boeri) – Trieste –
February 5th, 2008
SEMINAR – X-ray study of MEMS structures (A. Dommann) – Trieste – February 18th,
2008
SEMINAR – First-Principle investigations of intrinsic and Si-doped GaAs nanowires (N.
Gaderi) – Trieste – February 29th, 2008
SEMINAR – Spatially resolved spectroscopic characterization of cluster assembled
nanocomposite films (M. Amati) – Trieste – March 7th, 2008
SEMINAR – Unravelling the electronic structure of intercalated graphite: a key to
graphene and single-wall carbon nanotubes (A. Gruneis) – Trieste – March 12nd, 2008
SEMINAR – Characterization and manipulation of single molecules and molecular
complexes by Low-Temperature Scanning Tunneling Microscopy (C. Dri) – Trieste –
April 2nd, 2008
SEMINAR – Bacterial protein crystals as pure biomimetic nanomolecules and other
biophysical issues (J.L. Toca-Herrera) – Trieste – April 4th, 2008
SEMINAR – Some new directions in materials and nanostructure studies with
photoemission and x-ray emission:instrumentation, standing waves and hard x-rays (C.
Fadley) – Trieste – April 14th, 2008
SEMINAR – Catalytic model systems studied by STM and photoemission (C. Blasetti) –
Trieste – April 16th, 2008
SEMINAR – Celle fotovoltaiche organiche nanostrutturate: prime misure e primi risultati
(E. Sovernigo) – Trieste – April 16th, 2008
SEMINAR – Evaporation driven self assembly of colloidal/nano particles during spray
drying: A SAXS investigation (D. Sen) – Trieste – April 18th, 2008
SEMINAR – Photoelectron spectroscopy of reactive intermediates with synchrotron
radiation (F. Innocenti) – Trieste – April 24th, 2008
SEMINAR – Magnetism atom by atom: Magnetic states where you would not expect
them (S. Modesti) – Trieste – May 7th, 2008
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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SEMINAR – Molecular motors at surfaces (P. Rudolf) – Trieste – May 8th, 2008
SEMINAR – Catalyst assisted and catalyst free growth of GaAs nanowires by molecular
beam epitaxy (MBE) (F. Jabeen) – Trieste – May 8th, 2008
SEMINAR – Electronic states of Cu vicinal surface nanopatterned by oxygen-induced
reconstruction (C.E. Viol Barbosa) – Trieste – June 11th, 2008
SEMINAR – Catalytic combustion of methane. A case study: Pd/CeO2 catalysts (S.
Colussi) – Trieste – June 23rd, 2008
SEMINAR – Mechanisms of high-order photoemission from metal surfaces (F. Bisio) –
Trieste – June 24th, 2008
SEMINAR – Covalent connection of organic molecules on Au(111) (L. Lafferentz) –
Trieste – July 4th, 2008
SEMINAR – Imaging and force spectroscopy by AFM on DNA (G. Di Santo) – Trieste –
July 7th, 2008
SEMINAR – Polyoxometalates: from Molecular Catalysts to Nanostructures, towards
Innovative Materials (M. Bonchio) – Trieste – July 9th, 2008
SEMINAR – Nanofabrication strategies for the development of new tools for biosensing
(G. Rius Suñé) – Trieste – July 14th, 2008
SEMINAR – Growth, Properties Measurements and Nanodevices Applications of 1D
Nanoclusters (J. Tian) – Trieste – September 12th, 2008
SEMINAR – Production and characterization of 3D metal nanoparticle films (S.
D‟Addato) – Trieste – November 17th, 2008
SEMINAR – Bulk and supported oxides as catalysts for gas-phase reactions:
Characterization and reactivity of surface species (F. Cavani) – Trieste – February 24th,
2009
SEMINAR – Low-dimensional oxide structures on metal surfaces (S. Surnev) – Trieste –
March 6th, 2009
SEMINAR – Transducing biomolecular interactions into nanomechanics (P. Bergese) –
Trieste – March 10th, 2009
SEMINAR – Linear and nonlinear spectroscopy of a single metal nanoparticle (N. Del
Fatti, F. Vallée) – Trieste – April 16th, 2009
SEMINAR – Nanothermodynamics applied to the local thermal properties of disordered
materials (R.V. Chamberlin) – Trieste – May 14th, 2009
Support educational activity and teaching
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Candidate Barbara Lorenzut – [email protected]
Title of the thesis: “Development of nanostructured catalysts for hydrogen production and
purification”
Supervisor: Prof. Paolo Fornasiero
Tutor: Dr. Tiziano Montini
Research Activity
During the three years of PhD, catalytic systems for hydrogen production and purification were
synthetized and characterized. In the first year, catalysts based on transition metal supported on
ZnO/Al2O3 were developed for methanol and ethanol steam reforming. The activity was carried out
in collaboration with ACTA s.p.a.. This collaboration was continued during the second year, when
the research activity was directed to the development of systems based on ruthenium embedded into
porous oxides for hydrogen production from ammonia cracking reaction. Finally, during the last
year, the complete characterization of the developed catalysts confirmed that the embedded
approach leads to nanostructured catalysts with very good catalytic activity and high thermal
stability. Moreover, with the scientific collaboration of ACTA s.p.a, non noble metal catalysts (Fe
and Mo based) were synthesized for ammonia cracking. These Fe-Mo based catalysts were prepared
by classical impregnation method with great attention to the role of either the metal and the support.
The effect of K doping on the catalytic activity and stability was considered on the most active
systems for NH3 cracking; enhancement of the catalytic activity was observed only for catalyst
prepared by co-impregnation with salt of potassium at low concentration (< 0.5%). During the third
PhD year, a new project concerning the development of catalysts for glycerol reforming was also
started. Pt based catalysts supported on Al2O3 were developed, characterized and tested. The
introduction of CeO2 and La2O3 as dopants increases the H2 yields and the thermal stability in
reaction conditions.
Part of the results obtained in collaboration with ACTA s.p.a. is still confidential and it will not be
presented during the final examination.
Publications on scientific journals (printed or in press)
Italian Patent FI2007A0000179 “Catalizzatori per la produzione di gas di sintesi da reforming
di idrocarburi o alcoli comprendenti un supporto in ZnO e loro uso” ; Inventors: Paolo
BERT, Stefano CATANORCHI, Paolo FORNASIERO, Mauro GRAZIANI, Barbara
LORENZUT, Hamish MILLER, Tiziano MONTINI, Marina RAGNOLI, Adriana
SCAFFIDI, Alessandro TAMPUCCI.
Loredana De Rogatis, Tiziano Montini, Barbara Lorenzut and Paolo Fornasiero, Energy
Environ. Sci., 2008, 1, 501 – 509 “NixCuy/Al2O3 based catalysts for hydrogen production”.
T. Montini, A. Speghini, L. De Rogatis, B. Lorenzut, M. Bettinelli, M. Graziani and P.
Fornasiero, “The identification of the structural phases of CexZr1-xO2 by Eu(III)
luminescence studies.”, Journal of the American Chemical Society 131 (2009), 13155-13160
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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S. Specchia, L. D. Vella, B. Lorenzut, T. Montini, V. Specchia and P. Fornasiero, “Effect of the
catalyst load on syngas production in SCT-CPO reactors.”, Industrial and Engineering
Chemistry Research (2009), accepted for publication, doi:10.1021/ie900695j.
L. De Rogatis, M. Cargnello, V. Gombac, B. Lorenzut, T. Montini and P. Fornasiero,
“Embedded phases: a way to active and stable catalysts”, ChemSusChem (2009), accepted
for publication
Publications/abstracts in conferences/congresses (national or international)
Meeting ACTA, 25 th -27 th October 2007, Lavoria (PI)- Presentation of obtained results
“Development of nanostructured catalysts supported on ZnO for H2 production from ethanol
steam reforming”
XII National Congress of Industrial Chemistry – “Energy, materials and products from ecosustainable technologies and processes” – Genova 30 th June-3 th July 2008. Oral
communication “H2 production from ethanol and methanol steam reforming over Co, Ni, Cu
based catalysts”.
Catalysis interdivisional Group School of Italian Chemical Society “From catalyst to process:
principles, strategies and ways”– Certosa di Pontignano (SI) 3 th -6 th November 2008 –
Poster communication “Influence of preparation method on performance of Co, Ni, Cu
based catalysts for methanol and ethanol steam reforming for H2 production”
Meeting ACTA, 12 th November 2008, Lavoria (PI)- Presentation of obtained results
“Development of Ru embedded nanostructured catalysts for ammonia decomposition”.
CLEAR Summer School in Catalysis 2009 “Catalysis Lectures for Environmental Applications
& Renewables”- Porto Carras, Chalkidiki, Greece, May 24 th -29 th, 2009. Poste
communication “Ruthenium nanoparticles embedded catalysts for hydrogen production
from ammonia decomposition” Round table partecipation “Hydrogen production”.
Meeting ACTA, 24 th July 2009, Trieste- Presentation of obtained results “Development of
biomimetic catalysts Fe/Mo based for ammonia decomposition”.
Meeting ACTA, 4 th November 2009, Trieste- Final presentation on results obtained in
collaboration with ACTA S.p.a. during ammonia cracking project.
Educational Activity
Periods abroad (date and place)
CLEAR Summer School in Catalysis 2009 “Catalysis Lectures for Environmental Applications &
Renewables”- Porto Carras, Chalkidiki, Greece, May 24 th -29 th, 2009
Classes followed (date, course, professor, type of course)
“ARS2 II School: “ Sinchrotron radiation applicated on the nanostructured materials and thin
films characterization” Trieste, Adriatico Guesthouse, 22 th -26 th April 2007
Course of “Eterogeneous catalysis” Prof. Paolo Fornasiero (4CFU -36 h)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Course of “Inorganic chemistry I” Prof. Mauro Graziani (8CFU -64 h)
Course of “Advanced microscopies” at TASC laboratories Dr. Vincenzo Grillo – (6 h)
School of catalysis of the Italian Chemical Society- Certosa di Pontignano (SI), 3-6 November
2008
Course of “Structural chemistry” Prof. Lucio Randaccio (4CFU -36 h)
Course of “Synchrotron light characterization techniques” Prof. G. Vlaic
Course of “Inorganic chemistry II” Prof. M. Graziani
Conferences, seminars, advanced courses and other didactic activities
Conference: “Molecular Printboards, from supramolecular chemistry to nanofabrication”- Prof.
David N. Reinhoudt-Supramolecular Chemistry and Tecnology, Faculty of Science and
Tecnology, University of Twente, Olanda - il 5 th March 2007 - University of Trieste
Conference:” La sfida del secolo- Energia” –Dott. Piero Angela and Alberto Pinna - University
of Trieste
Conference: “ Per fortuna si innalza il prezzo del petrolio. Così la chimica riparte”- Prof.
Ferruccio Trifirò Industrial and materials chemistry Department, University of Bologna and
journal editor of “La Chimica e l‟Industria” University of Trieste
Conference: “Funcionalized gold nanoparticles: synthesis and applications”-Prof. Lucia
Pasquato- Chemical Sciences Department- University of Trieste
Conference: “ Carbonylation reaction in supercritical carbon dioxide”- Dr. Anna M. MasdeuBultò- Department de Quimica Fisica Inorganica, Universitat Rovira i Virgili, Terragona (
Spagna)- Chemical Sciences Department- University of Trieste
Conference: “Methane clatrate: an energy storage”- Prof. Giacomo Costa, Prof. Iginio Marson,
Dr. Umberta Tinivella, Prof. Lino Santoro, Dr. Fabio Pagan - University of Trieste
Congress Nanotech 2008 “Nanotechnology for Industry 2015” – Venice 10 th -13 th March 2008
“Workshop safety” organized by VWR - University of Trieste, 20 th May 2008
Conference: “Paints: Applied chemistry”- Dr. Gianpaolo Vicario – Elacrom Udine - 26 th
March 2008 - Chemical Sciences Department- University of Trieste
Conference: “Electronic Spin resonance Spectroscopy in Supramolecolar Chemistry” – Prof.
Marco Lucarini – Organic Chemistry Department “A. Mangini” University of Bologna – 4th
April 2008 - Chemical Sciences Department- University of Trieste.
Conference: “ Confined and composite soft matter on the nanoscale”- Prof. Marie Luise
Saboungi- CRMD, CNRS-Universitè d‟Orléans- 11 th April 2008 - University of Trieste
Conference: “Nanomaterials in catalysis: problems and possible solutions”- Dr. Massimiliano
Comotti – Acta s.p.a. – 21 th April 2008 - Chemical Sciences Department- University of
Trieste
Conference: “Theoretical simulation of condensed systems XAS spectra” - Prof. Giovanna
Fronzoni – Chemical Sciences Department- University of Trieste
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Formative lecture: “Safety practice in the gas employment”- organized by Sapio group - 26 th
November 2008 - Chemical Sciences Department- University of Trieste
Annual meeting PhD School in Nanotechnology, 13 th -15 th January 2009,
“Information meetings for doctoral students”, 30 th June 2009,
“Seminar on writing (and managing) a research project”, 16 th October 2009
Annual meeting PhD School in Chemical and Pharmaceutical Sciences and Technologies, 21th
-23 th January 2009.- University of Trieste
Conference: “Modelling reactions at the nanoparticle scale”, Prof. P. Westmoreland, 8 th May
2009 - University of Trieste
Conference: “Physical Chemistry nanostructured systems interphases”, Dr. I. Colombo, 15 th
May 2009- University of Trieste
Conference: “Penta coordinated Pd complexes and bi-functional effects in organometallic
chemistry”, Prof. F. Ragaini, 30 th June 2009 - University of Trieste
Conference “NMR Techniques for Investigating the Supramolecular Structure in Solution”,
Prof. Alceo Macchioni, 17 th September 2009 - University of Trieste
Conference. “Nanoparticles dissolution and absorption: biopharmaceutics, physical pharmacy
and simulation”, Prof. Michael B. Bolger, 25 th September 2009
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Candidate GIOVANNI MARIA PAVAN
Title of the thesis: structural and energetic analysis of organic and inorganic systems. The molecular
modelling potentiality.
Supervisor: Prof. Sabrina Pricl
Tutors (if any):
Research Activity
During the first year of activity, the subject of the study has been the “target therapy” in
oncology, particularly related to protein-drug systems characteristic of diseases as chronic
myeloid leucemia (LMC) and gastrointestinal stromal tumors (GISTs) using molecular
simulations methods in the framework of a collaboration with the Istituto Tumori of Milan.
During the second year, the attention was pointed to the study of the c-KIT complex, often
mutated in GIST, with various inhibitors in another collaboration with the M.D. Anderson
hospital of Houston (TX, USA). In this framework we performed studies on primary and
secondary mutations and direct energetic and structural comparison between complexes
composed of c-KIT with two inhibitor drugs (imatinib and dasatinib), and the therapy
response. In the last year of activity, the study of the KIT L576P mutation, present in few
rare tumors, was investigated further and, together with the Istituto Tumori of Milan, we
explored the causes of such decreased affinity for imatinib reported by this mutation. This
study provided very interesting results, highlighting how other inhibitors (sorafenib and
nilotinib) can show the same affinity problems of imatinib with kinases, being their
penetrations in the binding pocket of c-KIT extremely similar. On the contrary, drugs like
dasatinib and sunitinib, that bind rather differently with the receptor, seem not to be affected
by the presence of the L576P mutation. In conclusion, the study of a set of several mutations
on the c-KIT-imatinib complex, again in collaboration with the M.D. Anderson hospital,
evidenced an optimal accordance between the results obtained in silico and the clinical
evidences, demonstrating the reliability and the potentiality of molecular simulation as a
powerful tool for biomedical survey.
Publications on scientific journals (printed or in press)
1. E. Conca, T. Negri, A. Gronchi, E. Fumagalli, E. Tamborini, G.M. Pavan, M. Fermeglia,
M.A. Pierotti, S. Pricl, S. Pilotti. Activate and resist: L576P-KIT in GIST. Molecular
Cancer Therapeutics. Mol Cancer Ther 1535-7163.MCT-09-0662. Mol Cancer Ther
8(9):2491-5, 2009.
2. S.E. Woodman, J.C. Trent, K. Stemke-Hale, A.J. Lazar, S. Pricl, G.M. Pavan, M. Fermeglia,
Y.N. Gopal, D. Yang, D.A. Podoloff, D. Ivan, K.B. Kim, N. Papadopoulos, P. Hwu, G.B.
Mills and M.A. Davies. Activity of dasatinib against L576P KIT mutant melanoma:
Molecular, cellular, and clinical correlates. Mol Cancer Ther 8(8):2079-85, 2009.
3. T. Negri, G.M. Pavan, S. Pricl, S. Lagonigro, F. Miselli, A. Greco, M. Fermeglia, M.A.
Pierotti, S. Pilotti, E. Tamborini, T670X KIT mutations in GISTs: making sense of
missense. J Natl Cancer Inst 2009;101:1–11.
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4. J.C. McAuliffe, W.L. Wang, G.M. Pavan, S. Pricl, D. Yang, S.S. Chen, A.J.F. Lazar, R.E.
Pollock, J.C. Trent. Unlucky number 13? Differential effects of KIT exon 13 mutation in
gastrointestinal stromal tumors. Molecular Oncology. August 2008. Vol. 2, Issue 2,
Pages 161-163.
5. P. Posocco, G.M. Pavan, G. Scocchi, J.W. Handgraaf, A. Malek, M. Maly, M. Fermeglia,
J.G.E.M. Fraaije, C.V. Catapano, A. Danani, S. Pricl. Base Invaders. Coupling
Experiments and Multiscale Modeling of Dendrimer-Based siRNA Delivery Agents.
Advances in Science and Technology. 2008, 57: 154-159.
Publications/abstracts in conferences/congresses (national or international)
1. S.E. Woodman, J.C. Trent, K. Stemke-Hale, A. Lazar, S. Pricl, G.M. Pavan, N.
Papadopoulos, P. Hwu, G.B. Mills, M.A. Davies. Selective Activity of Dasatinib for the
Most Common KIT Mutation in Melanoma (L576P). Journal of Clinical Oncology, 2009
ASCO
Annual
Meeting
Proceedings
(Post-Meeting
Edition).
Vol 27, No 15S (May 20 Supplement), 2009: 9019.
2. S. Pricl, G.M. Pavan, M. Fermeglia, E. Tamborini, M.A. Pierotti, S. Pilotti. EGFR
mutations and gefitinib affinity: molecular insights from in silico experiments.
European Journal of Cancer Supplements, Volume 6, Issue 12, October 2008, Page 154.
EORTC-NCI-AACR 2008. Geneva, 21-24 October 2008.
3. S. Pricl, E. Tamborini, M. Ferrone, G.M. Pavan, T. Negri, M.A. Pierotti, S. Pilotti. A
clinical/modelling investigation of the activity of PKC412 against an imatinib resistant
gastrointestinal stromal tumor (GIST) carrying a D842V mutation in PDGFRA. AACR
2007 – Los Angeles.
Educational Activity
Periods abroad (date and place)
Classes followed (date, course, professor, type of course)
Conferences, seminars, advanced courses and other didactic activities
1.
2.
3.
4.
5.
6th International Dendrimer Symposium. Stockholm, 14-18 June 2009.
Trans-alp Nano 2008. Lione, 27-29 October 2008.
BIO-dendrimer 2008 – Lodz, Poland, 03-05 July 2008.
1st European Conference for Clinical Nanomedicine – Basel, 18-21 May 2008.
II Scuola ARS2 - Applicazioni della radiazione di sincrotrone allo studio dei materiali
nanostrutturati e dei film sottili - Grignano (TS), 22-26 aprile 2007
Support educational activity and teaching
Molecular Modelling class (Prof. Sabrina Pricl), module of “ab inizio Methods for
Molecular Modelling”.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Candidate PAOLA POSOCCO
Title of the thesis: Multiscale molecular simulation of nanostructured systems
Supervisor: prof. M. Fermeglia
Tutors (if any): prof. S. Pricl
Research Activity
In recent years considerable interest and economic resources have been devoted to the
nanotechnology and particularly to nanostructured materials by the European and international
community. Their potential is related to the fact that these materials posses a structure at the
nanometer level which confers macroscopic properties not comparable or detectable in common
materials. To date, the knowledge of the properties and characteristics at the nanometric level and
the resulting relations with the macroscopic performances achieved by the material are limited.
Molecular simulation techniques are valuable tools that can be used not only to increase the
understanding of phenomena at the molecular level, but also as a support for more efficient and
sustainable design of new systems.
The aim of the research was to develop novel procedures in the framework of a multiscale
simulation approach to be applied to systems of industrial interest, with the ultimate goal of creating
an effective tool effectively for the design and implementation of new nanostructured materials. The
systems analyzed belong to different fields, ranging from polymeric nanocomposite materials, to
epoxy resins, block copolymers, and gels for biomedical applications, in order to demonstrate the
broad applicability of the developed methodology.
As an example, the key results obtained in the case of three systems are summarized briefly: (i)
block copolymers (polystyrene-poly(vinylpyridine)) loaded with metal nanoparticles of gold, (ii)
cross-linked epoxy resins (based on glycidoxy propyl silane) with nanoparticles of titanium oxide,
(iii) composite polymers (polyethylene oxide, nylon 6, …) in the presence of lamellar silicates
(montmorillonite).
(i) The morphology of the copolymer, when loaded with metallic particles functionalized with one
or both polymeric building blocks in different percentages, has been predicted. In particular, the
effect of concentration, degree and type of covering (enthalpic effect) and shape (entropic effect) of
the nanoparticle on the morphology of the final composite was assessed, highlighting the
thermodynamic parameters that can be used to control the dispersion of the metal in the polymer.
(ii) A code for the simulation of the crosslinked matrix was developed, and the thermo-mechanical
characteristics as a function of the degree of crosslinking achieved during the reaction has been
evaluated. Accordingly, it has been possible to predict the mechanical properties of composite
material as a function of volume concentration of nanofiller.
(iii) A procedure has been create to calculate macroscopic properties of the nanocomposite
materials (such as mechanical properties - Young‟s modulus, shear modulus, bulk modulus,
Poisson's ratio – thermal properties- thermal expansion coefficient- electrical properties – electrical
conductivity) depending on the concentration of clay and the quality of the dispersion starting only
from molecular information of the material. According to the conceived computational recipe, no
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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experimental data are required as input at any scale level. In addition, we have analyzed the
molecular interactions between the components of the system and assessed the impact of those on
the final properties of the material, providing a tool for a better evaluation and a priori selection of
each component.
Publications on scientific journals (printed or in press)
1.
Toth R, Voorn DJ, Handgraaf JW, Fraaije JGEM, Fermeglia M, Pricl S, Posocco P.
Multiscale computer simulation studies of water-based montmorillonite/poly(ethylene oxide)
nanocomposites. Macromolecules, 2009, on web (ma200901584w).
2.
Scocchi G, Posocco P, Handgraaf JW, Fraaije JGEM, Fermeglia M, Pricl S. A complete
multiscale modelling approach for polymer-clay nanocomposites. Chemistry - A European
Journal, 15, 7586-7592, 2009.
3.
Posocco P, Lisal M, Posel Z, Fermeglia M, Pricl S. A multiscale molecular simulation
approach to the prediction of the morphology of self-assembled nanoparticles in diblock
copolymers. Journal of Physical Chemistry B, submitted.
4.
Posocco P, Fermeglia M, Pricl S. A multiscale toolbox for morphology prediction of diblock
copolymers for drug delivery. Biomacromolecules, submitted.
5.
Fermeglia M, Maly M, Posocco P, Pricl S. Multiscale Molecular Modeling of Hybrid
Organic-Inorganic Nanocomposites of Type I and II. Advances in Science and Technology,
54, 265-269, 2008.
6.
Maly M, Posocco P, Fermeglia M, Pricl S. Scripting approach in hybrid organic-inorganic
condensation simulation: the GPTMS proof-of-concept. Molecular Simulation, 34, 12151236, 2008.
7.
Maly M, Posocco P, Pricl S, Fermeglia M. Self-assembly of nanoparticle mixtures in diblock
copolymers: multiscale molecular modeling. Industrial & Engineering Chemistry
Research, 47, 5023-5038, 2008.
8.
Scocchi G, Posocco P, Fermeglia M, Pricl S. Polymer-Clay Nanocomposites: A Multiscale
Molecular Modeling Approach. Journal of Physical Chemistry B, 111, 2143-2151, 2007.
9.
Scocchi G, Posocco P, Pricl S, Fermeglia M. To the nanoscale, and beyond! Multiscale
molecular modeling of polymer-clay nanocomposites. Fluid Phase Equilibria, 261, 366-374,
2007.
10.
Posocco P, Ferrone M, Fermeglia M, Pricl S. Binding at the core. Computational study of
structural and ligand binding properties of naphthiridine based dendrimers.
Macromolecules, 40, 2257-2266, 2007.
Publications/abstracts in conferences/congresses (national or international)
1.
Posocco P, Fermeglia M, Pricl S. Multiscale molecular modeling of di/triblock copolymers for
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
23
drug delivery. FOMMS, Blaine, USA, (2009).
2.
Fermeglia M, Posocco P, Pricl S, Fraaije JGEM, Handgraaf JW, Scocchi G, Danani A. A
complete multiscale modeling approach for nanocomposites. Eurofillers, Alessandria, Italy,
(2009).
3.
Fermeglia M, Posocco P, Pricl S, Fraaije JGEM, Handgraaf JW, Deglmann P, Kurkal-Siebert
V, Weiss H. On the DPD Parameter Estimation From Atomistic/Quantum Mechanics
Information. AIChE Annual meeting, Nashville, USA, (2009).
4.
Fermeglia M, Scocchi G, Pricl S, Handgraaf JW, Fraaije JGEM, Posocco P. A Complete
Multiscale Modeling Approach for Polymer-Clay Nanocomposites. AIChE Annual meeting,
Nashville, USA, (2009).
5.
Posocco P, Fermeglia M, Pricl S. The Long and Winding Road. Multiscale Molecular
Modeling Approach to the Self-Assembly of Di/Triblock Copolymers for Drug Delivery in
Aqueous Solution. AIChE Annual meeting, Nashville, USA, (2009).
6.
Posocco P. Modellistica molecolare multiscala per la progettazione di materiali
nanostrutturati. I giovani e la chimica in FVG, Trieste, (2009).
7.
Posocco P, Handgraaf JW, Fraaije JGEM, Scocchi G, Fermeglia M, Pricl S. A multiscale
approach to the self-assembly of dendrimers in solution, CECAM, Lausanne, Switzerland,
(2008).
8.
Fermeglia M, Pricl S, Posocco P, Mály M, Lisal M. Computer Simulation of Hybrid OrganicInorganic Nanocomposites, AICHE Annual meeting, Philadelphia, USA, (2008).
9.
Pricl S, Posocco P, Mály M, Lisal M, Fermeglia M. Beyond Patterns to Mechanisms.
Multiscale Molecular Simulations of Nanoparticles/block Copolymers Self-Assembled Bulk
Nanocomposites, AICHE Annual meeting, Philadelphia, USA, (2008).
10.
Posocco P, Scocchi G, Fermeglia M, Handgraaf JW, Fraaije JGEM, Pricl S. A Complete
Multiscale Modeling Approach for Nanocomposites, AICHE Annual meeting, Philadelphia,
USA, (2008).
11.
Fermeglia M, Posocco P, Pricl S, Maly M. Multiscale Molecular Modeling of Hybrid
Organic-Inorganic Nanocomposites of Type I and II, 12th International Conferences on
“Modern Materials and Technologies” CIMTEC, Acireale, Italia, (2008).
12.
Posocco P, Toth R, Voorn DJ, Handgraaf JW, Fermeglia M, Pricl S. Paving the way to new
nanocomposite materials: multiscale computer simulations of PEO and exfoliated layer
silicates in aqueous dispersions, 1st Transalp' Nano Conference, Lyon, France, (2008).
13.
Paneni M, Posocco P, del Santo I, Causa F, Netti PA, Pricl S. “Math”-odological insights into
smart nanochannels: a multiscale molecular modeling of molecular motion in confined
nanospaces, 1st Transalp' Nano Conference, Lyon, France, (2008).
14.
Scocchi G, Posocco P, Pricl S, Fermeglia M. To the nanoscale and beyond! Multiscale
molecular modelling of polymer-clay nanocomposites, 11th International Conference on
“Properties and Phase Equilibria for Product and Process Design” PPEPPD, Crete, Greece,
(2007).
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
24
15.
Posocco P, Scocchi G, Fermeglia M, Pricl S, Ferrari M. The nanoroad less travelled. A
multiscale approach to self-assembly of dendrimers for cancer therapy, 11th International
Conference on “Properties and Phase Equilibria for Product and Process Design” PPEPPD,
Crete, Greece, (2007).
16.
Fermeglia M, Posocco P, Maly M, Pricl S. Enthalpy/entropy effect in the self assembly of a
nanoparticle complex system: a multiscale molecular modeling (M3) approach. AIChE
Annual Meeting, Salt Lake City, UT, USA, (2007).
17.
Pricl S, Posocco P, Fermeglia M, Scocchi G, Danani A, Handgraaf JW, Fraaije JGEM. What
goes around comes around. Multiscale molecular modeling of dendrimers self-assembly,
IDS:5 fifth international dendrimer symposium, Tolouse, France, (2007).
18.
Cosoli P, Fermeglia M, Ferrone M, Posocco P, Pricl S, Scocchi G. New materials from
multiscale modeling procedures: properties prediction and customization of polymeric
nanocomposites, ICheaP-8, Ischia, Italia, (2007).
Books /Chapters and sections of books
1.
Pricl S, Posocco P, Scocchi G, Fermeglia M. “Polymer-clay nanocomposites”, in Handbook
of Nanophysics, Klaus Sattler Editor, Taylor&Francis Publisher, 2009.
Other publications
1.
Scocchi G, Posocco P, Ferrone M, Fermeglia M, Pricl S, Handgraaf JW, Fraije JGEM.
“Nanocompositi. Da micro a mesoscala”, Plastix, (2007), 9, 26.
1.
Participation to conferences (as speaker)
Posocco P, Fermeglia M, Pricl S. Multiscale molecular modeling of di/triblock copolymers for
drug delivery. FOMMS, Blaine, USA, (2009).
2.
Posocco P, Fermeglia M. Self-assembly of nanoparticle mixtures in diblock copolymers. 2nd
PhD Workshop, Wittenberg, Germany, (2009).
3.
Posocco P. Modellistica molecolare multiscala per la progettazione di materiali
nanostrutturati. I giovani e la chimica in FVG, Trieste, (2009).
4.
Posocco P, Handgraaf JW, Fraaije JGEM, Scocchi G, Fermeglia M, Pricl S. A multiscale
approach to the self-assembly of dendrimers in solution, CECAM, Lausanne, Switzerland,
(2008).
5.
Posocco P, Toth R, Voorn DJ, Handgraaf JW, Fermeglia M, Pricl S. Paving the way to new
nanocomposite materials: multiscale computer simulations of PEO and exfoliated layer
silicates in aqueous dispersions, 1st Transalp' Nano Conference, Lyon, France, (2008).
6.
Posocco P, Scocchi G, Fermeglia M, Pricl S, Ferrari M. The nanoroad less travelled. A
multiscale approach to self-assembly of dendrimers for cancer therapy, 11th International
Conference on “Properties and Phase Equilibria for Product and Process Design” PPEPPD,
Crete, Greece, (2007).
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Educational Activity
Periods abroad (date and place)
Classes followed (date, course, professor, type of course)
Conferences, seminars, advanced courses and other didactic activities
1.
2.
3.
4.
5.
6.
7.
8.
2nd PhD Workshop, 11-13 September 2009, Wittenberg, (Germany).
“How to write (and manage) a research project”, 16 October 2009, Trieste.
“Chimica-fisica delle intefasi in sistemi nanostrutturati”, 15 May 2009, Trieste.
Seminario VPN e Portale Scuola Nanotecnologie, 20 March 2009, Trieste.
“Modeling reactions at the nanoparticle scale”, 8 May 2009, Trieste.
“Culgi Advanced Course”, 8-10 April 2008, Leiden (Olanda).
II Scuola ARS2 - Applicazioni della radiazione di sincrotrone allo studio dei materiali
nanostrutturati e dei film sottili - 22-26 April 2007, Grignano (TS)
XXVII Convegno-scuola AIM 2007- Materiali polimerici ibridi e nanostrutturati- 30 April- 4
May 2007, Gargnano (BS).
Support educational activity and teaching
Dissipative Particle Dynamics methodology, course of Molecular Simulation (prof. Pricl), theory
and exercises.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
26
Candidate: ANDREA TRAVAN
Title of the thesis:
“Nanocomposite systems based on metal nanoparticles and polysaccharides for biomedical
applications”
Supervisor: Prof. Sergio Paoletti
Tutor: Dott. Ivan Donati
Research Activity
The Ph.D research project refers to the development of nanocomposite systems for biomedical
applications and is included in the framework of the European Project called “Newbone”; in
particular, the final goal is to realize coatings for orthopedic prosthesis endowed with antimicrobial
properties. Nanocomposite systems based on natural polysaccharides that stabilize metal
nanoparticles (silver and gold) in colloidal solutions and as three-dimensional hydrogels are been
studied, as antimicrobial agents. During the first and second year of the Ph.D we have set up the
protocols for the preparation of the colloidal solutions and of the hydrogels containing the metal
nanoparticles on the basis of physical-chemical and biological characterizations. Moreover we have
studied and defined a particular chemical mechanism of silver ions reduction carried out by a
lactose modified chitosan; the optical properties of the so-formed metallic nanoparticles obtained
via this mechanism were tested by means of Raman spectroscopy, thus detecting the possibility to
have considerable enhancements of the signal due to the SERS effect (Surface Enhanced Raman
Spectroscopy). Thanks to the antibacterial properties of silver, the nanocomposite gels display
potent bactericidal activity without showing cytotoxic effects towards eucariotic cells, as verified by
both in vitro and in vivo tests. This result is particularly important in relation to the state of the art in
this research field. Since the core material of the prosthesis is realized in methacrylic materials,
during the third year of Ph.D we have devised a technique for coating of substrates based on its
activation followed by deposition of the bioactive nanocomposite due to introduction of negative
charges on the substrate surface, the coating is grafted by means of electrostatic interactions with
the polysaccharide chains. Biological in vitro tests have demonstrated that the antimicrobial activity
persists together with the lack of cytotoxicity, determining the choice of this coating for the final in
vivo test as a conclusion of the European project. The main characterization techniques employed
were UV-Vis and Raman spectroscopy, TEM, SEM and AFM microscopy, Confocal Fluorescence
Microscopy, Mechanical bending tests, ICP-MS as well as other cellular and microbiological
techniques. The results obtained during this Ph.D have been published on scientific journals, books,
abstracts, posters and proceedings of International Congresses; based on theses results two Italian
Patents were filed and the PCT extension is being processed.

Publications on scientific journals (printed or in press)
Polyol Synthesis of Silver Nanoparticles: Mechanism of Reduction by Alditol Bearing
Polysaccharides (Ivan Donati, Andrea Travan, Chiara Pelillo, Anna Coslovi, Alois
Bonifacio,Valter Sergo, Sergio Paoletti). Biomacromolecules, 2009, 10 (2), 210-213
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Non-Cytotoxic Silver Nanoparticle – Polysaccharide Nanocomposites with Antimicrobial
Activity (Andrea Travan, Chiara Pelillo, Ivan Donati, Eleonora Marsich, Monica Benincasa,
Tommaso Scarpa, Sabrina Semeraro, Gianluca Turco, Renato Gennaro, Sergio Paoletti).
Biomacromolecules 2009, 10, 1429–1435
Surface Modification and Polysaccharide Deposition on BISGMA/TEGDMA Thermosets
(Andrea Travan, Ivan Donati, Eleonora Marsich, Francesca Bellomo, Satish Achanta,
Sabrina Semeraro, Tommaso Scarpa, Mila Toppazzini, Vittorio Spreafico, and Sergio
Paoletti). Biomacromolecules 2010. (DOI: 10.1021/bm9011419)
Silver-Polysaccharide Nanocomposite Antimicrobial Coatings for Methacrylic Thermosets
(Andrea Travan, Eleonora Marsich, Ivan Donati, Monica Benincasa, Marta Giazzon, Laura
Felisari, Sergio Paoletti. Submitted to European Cells and Materials Journal (2010)
Characterization of silver nanoparticles for biomedical applications by means of
quantitative analysis of TEM micrographs (Francesco Brun, Andrea Travan, Agostino
Accardo, Sergio Paoletti). Biomedical Sciences Instrumentation 2010 (In press).

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

Publications/abstracts in conferences/congresses (national or international)
Silver Nanoparticles for Antimicrobial Applications (World Biomaterials Congress
Amsterdam 2008, abstract and poster)
Polysaccharide – Based Silver Nanoparticles Systems (World Biomaterials Congress
Amsterdam 2008, abstract and poster)
Characterization of Composite Polysaccharide-Based Scaffolds (World Biomaterials
Congress Amsterdam 2008, abstract and poster)
Silver Nanoparticle – Polysaccharide Nanocomposites with Antimicrobial Activity
(European Materials Research Society – EMRS - Fall Meeting, Warsaw 2008, abstract and
poster)
Silver Nanoparticles – Polysaccharides Based Systems (National Biotechnology Congress,
Perugia 2008, abstract and poster)
Non-Cytotoxic Silver Nanoparticle – Polysaccharide Nanocomposites with Antimicrobial
Activity (European Polymer Congress, Graz 2009, abstract and poster )
Silver nanoparticle - Polysaccharide systems for biomaterials applications (European
Polysaccharide Network of Excellence, Turku 2009, abstract and poster)
Nanocomposite systems based on polysaccharides and silver nanoparticles.
(Nanotechnology for the study of cellular and molecular interactions, Engineering
Conference International, Barga 2009, abstract and poster)
Porous Nano-Hydroxyapatite/Alginate Scaffolds for Bone Regeneration. (Nanotechnology
for the study of cellular and molecular interactions, Engineering Conference International,
Barga 2009, abstract and poster)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
28
Books /Chapters and sections of books









Silver nanocomposites and their biomedical applications (Andrea Travan, Eleonora
Marsich, Ivan Donati, Sergio Paoletti). Chapter of the book entitled “Nanocomposites for
the Life Sciences”. In press, Wiley-VCH (2010)
Other publications (Patents filed)
Materiali nanocompositi basati su nanoparticelle metalliche stabilizzate con polisaccaridi a
struttura ramificata (Andrea Travan, Ivan Donati, Eleonora Marsich, Sergio Paoletti,
Brevetto 8853 PTIT)
Materiali nanocompositi formati da una matrice polisaccaridica e nanoparticelle
metalliche, loro preparazione ed uso (Andrea Travan, Ivan Donati, Eleonora Marsich,
Sergio Paoletti, Brevetto 8981 PTIT)
Nanocomposite materials based on metallic nanoparticles stabilized with branched
polysaccharides (Andrea Travan, Ivan Donati, Elonora Marsich, Sergio Paoletti). PCT
Patent extension (2009)
Three-dimensional nanocomposite materials consisting of a polysaccharidic matrix and
metallic nanoparticles, preparation and use thereof (Andrea Travan, Ivan Donati, Elonora
Marsich, Sergio Paoletti). PCT Patent extension (2009)
Participation to conferences (as speaker)
Silver Nanoparticle – Polysaccharide Nanocomposites with Antimicrobial Activity CIRMIB
Biomaterials School. Nanomedicine: Imaging, Drug Release, Regenerative Medicine
(Ischia 2007)
Nanocomposite systems based on polysaccharides and silver nanoparticles.
(Nanotechnology for the study of cellular and molecular interactions, Engineering
Conference International, Barga, 2009)
Silver nanocomposites for biomaterials applications. (Trondheim-Trieste Bilateral
Workshop on Biopolymers, 2009)
Non-Cytotoxic Silver Nanoparticle – Polysaccharide Nanocomposites with Antimicrobial
Activity (European Polymer Congress, Graz 2009)
Educational Activity

Periods abroad (date and place)
22 march – 22 may (2009), Centre Suisse d' Electronique et Microtechnique (CSEM),
Neuchatel, Switzerland.

Classes followed (date, course, professor, type of course)
56 hours, Introduction to Biochemistry, march-june 2007, Prof. Ivan Donati (corso di laurea
in Biotecnologie)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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
48 hours, Organic Chemoistry, october 07-february 08, Prof. Fabio Benedetti (corso di
laurea in Biotecnologie)



Conferences, seminars, advanced courses and other didactic activities
Antibacterial Materials Congress (London 2007)
Ceramic, cells and tissues: Nanotechnology for the functional repair and regenerative
medicine (Faenza 2007)
Scuola ARS2: Applicazione della radiazione di sincrotrone allo studio dei materiali
nanostrutturati e dei film sottili (Trieste 2007)
Confined and composite soft matter on the nanoscale (M. Saboungi, 11/04/08)
Scanning Probe Microscopies (SPMs), Agilent workshop al TASC (11 June 2008)
Applications of near field Raman spectroscopy (G. Picardi-22 Maggio 2008)


Nanochallenge and Polymerchallenge (4 Luglio 2008)
Modern Trends in Nanobiology and Nanomedicine (Workshop, 13-14 october)

Image Analysis and Biomechanical Modelling: From Biomolecules to Bioactive
Biomaterials (Trieste 1st-2nd September 2008)
Mastering interfaces in composite and nanostructured materials: advances in processing
and characterization methods (COST Training School, May 4-8 2009, Thun, Switzerland
2009)
Fracture physics of biopolymer gels (Prof. Tristan Baumberger, Institut des NanoSciences
de Paris, France) (20 ottobre 2009)
Seminar on how to write and manage a research project (School of Nantochnology, Trieste






ottobre 2009)
Support educational activity and teaching
Co-tutor of a Master Thesis in Medical Biotechnologies (A. Di Luca: “Nanocomposite systems
based on polysaccharides and metal nanoparticles for biomedical applications”) and of two
Diploma Thesis in Biotechnologies (M. Ricciarelli: “Nanocomposite systems based on gold
nanoparticles and polysaccharides for biomedical applications”, E. Minighin : “Idrogels and
nanocomposite coatings based on polysaccharides and metal nanoparticles for biomedical
applications”).
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
30
Candidate ANDREA UMERI
Title of the thesis: Stress state and crystallographic analysis in relation to the ageing process in submicron composite Zirconia - Alumina
Supervisor: Prof. Valter Sergo
Tutors (if any):
Research Activity
The focus of research concerns the analysis of composites Alumina - Zirconia in relation to the
phenomenon of aging of zirconium, in light of the renewed interest of this material in dental field.
The zirconia was mentioned by several articles of literature in dentistry as a main material for the
construction of dentures, but these articles do not deal in sufficient depth the issue of ageing and its
consequences.
In the first phase of the research work were analyzed several samples, having undergone a natural
aging in the atmosphere of about 10 years, differentiated by size of ceramic powders and the initial
volumetric
rate
of
aluminum.
In the second phase was analyzed a second set of samples made with the aim of verifying the actual
aging of the composite through an artificial aging process that is followed by a third set of samples
which vary in the relative amounts of alumina and zirconia. In both cases it was monitored the
development in time of phase transformation by Raman spectroscopy. Through the simultaneous
use of AFM and NSOM has developed a method for selective identification of each grain in order
to Raman measurements at the nanoscale in single point. (In collaboration with the Dental Clinic
and stomatology Ospedale Maggiore in Trieste)
Publications on scientific journals (printed or in press)
Journal of Materials Science, “Raman investigation of the ageing of Ni-BaTiO3
multilayer ceramic capacitors”, A.Umeri, T.A. Kuku, N.Scuor and V.Sergo, Volume 43,
Issue 3, P.922-926,2008-02-01, DOI - 10.1007/s10853-007-2215-4
Publications/abstracts in conferences/congresses (national or international)
POSTER - “Nanostructured Zirconia in dental applications: new problems for an old
material” – A.Umeri, A. Bonifacio and V. Sergo – National Nanomedicine Conference –
28,29 November 2008 - Genova
Books /Chapters and sections of books
Other publications
Participation to conferences (as speaker)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
31
Educational Activity
Periods abroad (date and place)
Classes followed (date, course, professor, type of course)
Conferences, seminars, advanced courses and other didactic activities
Advanced Course – “6th National course of concrete technology” – 83 hours of lessons,
Engineering Faculty of University of Bergamo.
Workshop: “Smart Energy systems and fuel cells: on path to a sustainable future” – Mechanical
engineering department of University of Trieste
Scientific Congress: Young researchers and Chemistry in Friuli Venezia Giulia – University di
Trieste
Seminar: Prof. Fermeglia “VPN – Virtual Private Networking” – Univ. of Trieste
Seminar: Prof. Fermeglia, Prof. Sergo “How to write and manage a research project” – Univ. of
Trieste
Seminar: Prof. Phillip R. Westmoreland, Dept. of Chemical Engineering, University of
Massachusetts Amherst : “Modeling reactions at the nanoparticle scale”
Seminar: dott. Colombo “Chemistry and Physics of inter-phases in nanostructured systems” – Univ.
Of Trieste
Support educational activity and teaching
Main teacher of the course: “Materials and Renewable Energy in construction” Engineering
Faculty, University of Trieste, A.A. 2008/2009, 30 hours.
Teacher of the course “R.E.C.A. – Risparmio Energy saving and living comfort”, University of
Trieste, 2009, 10 hours.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
32
Dottorandi del 23 ciclo:
Graduate Student ALBERTO ASTOLFO
Title of the thesis: DEVELOPMENT OF NOVEL EXPERIMENTAL METHODS FOR
CELL TRACKING USING NANOPARTICLES
Supervisor: Dr. Fulvia Arfelli
Tutors (if any): Dr. Rer.Nat. Ralf-Hendrik Menk
Research Activity
The aim of this project is to develop novel experimental methods for cell tracking using
nanoparticles as contrast agent. The goal is to quantify and follow the cells during them life. The
using of nanoparticles (NPs) as well as a suitable tomographic X-ray imaging setup can be a good
solution of the problem above mentioned. During the past year we have done a double experiment
at the SYRMEP beamline (synchrotron radiation facility) and at the TOMOLAB laboratory
(microfocus X-Ray tube facility). The samples were 20 rat‟s heads ex-vivo that were injected into
the brain with the same amount of gold loaded C6 cells. The rats were sacrificed at different
moments in order to estimate the tumor visibility during its grow. The results‟ analysis has confirm
the cells‟ visibility obtained last year. We have found some troubles doing the quantitative analysis
due to some results in conflict caused by the small number of samples. We have found also another
problem related by the small bone pieces created during the hole drilling before the cell injection.
The instrumentation that we were using didn‟t allow us to separate the bone powder signal to the
marked cells signal. This can represent a technique limitation in this kind of experiments especially
if we want to count the cells. With the aim to better understand this problem, during the transfer at
the synchrotron Canadian Light Source (CLS, Saskatoon, Canada) we have prepared dedicated
samples containing the same kind of labeled cells and bone powder into agarose gel. At the CLS
I‟ve contributed to the set up of a tomography system. We have used it to scan also the samples
prepared. We have grown part of the same marked cells on a metalize cover slip in order to
visualize the cells on the Scanning Electron Microscopy (SEM). During the SEM measurements at
the TASC laboratories (Area di Ricerca, Basovizza) we have shown that it is possible to estimate
the amount of NPs on single cell. This is a different approach respect to the mass spectroscopy
approach that provides a mean gold weight of a large number of cells.
On the instrumental part of my work, during the last year, we have complete the new double crystal
analyzer setup for the Analyzer Based Imaging (ABI) now available at the SYRMEP beamline.
This setup permits new imaging modalities based on phase contrast instead on absorption contrast,
increasing the details visibility. During an experimental run dedicated, we have used the ABI to one
rat ex-vivo that was injected with marked C6 cells. Again at the SYRMEP beamline, we have
installed an experimental setup that permits to increase the imaging system resolution using the
beam diffraction of two silicon crystals cut asymmetrically (Bragg Magnifier). During this
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
33
experiment, we have done a tomography on one of the samples prepared in Canada (the C6 cells in
agarose gel) obtaining a single cell resolution (pixel size ~1 μm; PSF ~5 μm).
Objectives for the following year (if applicable)
Implementation of a cell counting software in IDL code (to be applied to samples with a well
known number of cells); studies and estimation of the NPs clusters partition during the mitosis
using SEM; new measurements of ABI (beamtime approved for the first part of 2010); tomographic
measurements on ex-vivo mice using marked stem cells (beamtime approved for the first part of
2010); implementation and use of “local area tomography” algorithms to scan with high resolution
only a zone of big samples; implementation and use of tomosynthesis algorithms to decrease the
mean dose to the sample; magnetic resonance imaging measurements and histological studies of
some already CT scanned samples to confirm what we have seen in CT (possible transfer at the
Australian Synchrotron in Melbourne). Implementation and use of a system for fluorescent
measurements.
Publications on scientific journals (printed or in press)
1. L. Rigon, A. Astolfo, F. Arfelli, R.H. Menk, “Generalized diffraction Enhanced imaging:
Application to tomography”, Eur J Radiol, Volume 68, Issue 3, Pages 3-7, 2008
2. L. Rigon, F. Arfelli, A. Astolfo, A. Bergamaschi, D. Dreossi, R. Longo, R.H. Menk, B.
Schmitt, E. Vallazza, E. Castelli, “A single-photon counting “edge-on” silicon detector
for synchrotron radiation mammography”, Nucl. Instr. And Meth. A, 608, S62-S65, 2009
Publications/abstracts in conferences/congresses (national or international)
1. Spatio-Temporal Radiation Biology: Transdiscipinary Advances for Biomedical
Applications – Booklet of abstracts (Saint Feliu de Guixols, Spain, 16-21 May 2009), A.
Astolfo, R.-H. Menk, F. Arfelli, E. Schultke, C. Hall, L. Rigon, B.H.J. Juurlink, “Long
Term Cell Tracking in Small Animals Phase Using Phase Contrast Based Micro CT and
Synchrotron Radiation”, P.53.
Books /Chapters and sections of books
Other publications
Participation to conferences (as speaker)
2. Poster at “IEEE Dresden 2008 Medical Imaging Conference” (Dresden, 19-25 October
2008), “Long Term Cell Tracking in Small Animals Phase Using Phase Contrast Based
Micro CT and Synchrotron Radiation”.
3. Poster at “physics@UniTS” (Trieste, 19 and 20 June 2008), “Generalized Diffraction
Enhanced X-ray Imaging: Application to Tomography”.
4. Short-Talk, “Spatio-Temporal Radiation Biology: Transdiscipinary Advances for
Biomedical Applications” (Saint Feliu de Guixols, Spain, 16-21 May 2009), “Long Term
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
34
Cell Tracking in Small Animals Phase Using Phase Contrast Based Micro CT and
Synchrotron Radiation”.
5. Talk, Canadian Light Source (Saskatoon, Canada, 28 August 2009), “Computer
Tomography @ BMIT – Present, future and resources at the Canadian Light Sources
Biomedical Beamline”
6. Talk, “Joint Workshop of SFB762 & IMPRS” (Wittenberg, Germany, 10-11 September
2009), “Development of novel experimental methods for cell tracking using
nanoparticles”.
Educational Activity
Periods abroad (date and place)
o 19-25 October 2008, Dresden, Germania, IEEE Medical Imaging Conference
o 16-21 May 2009, Saint Feliu de Guixols, Spain, Spatio-Temporal Radiation
Biology: Transdiscipinary Advances for Biomedical Applications
o 3 June-30 August 2009, Saskatoon, Canada, Canadian Light Source
o 11-12 September 2009, Wittenberg, Germania, Max Plank Institute
o 11-13 November 2009, Chicago, Illinois (USA), Advance Photon Source
Classes followed (date, course, professor, type of course)
o “Tomographic measurement with a microfocus X-ray tube”, February-March
2008, Dr. Ralf Menk, TOMOLAB at Elettra, 32 hours
o “Tomographic measurement with Synchrotron Radiation”, April 2008, Dr. Fulvia
Arfelli-Dr. Ralf Menk, SYRMEP at Elettra, 40 hours
o “Electron Microscopy and Scanning Probe Microscopy”, June-July 2008, Dr.
Cristina Africh-Dr. Laura Felisari, PhD School of Physics course, 16 hours
o “Metodi statistici in fisica”, September 2008, Dr. Paolo Schiavon, PhD school of
physics course, 9 hours
o “Tecnologie Cellulari”, October-December 2008, Dr. Roberto Marzari,
Functional Genomic course, 20 hours
o “Genetica Molecolare”, October-December 2008, Dr. Paolo Edoni, Functional
Genomic course, 38 hours
Conferences, seminars, advanced courses and other didactic activities
o “Aspetti principali della radioprotezione presso l‟INFN”, Prof. Franco Calligaris,
28 March 2008, Seminario, 2 hours
o “Workshop on nanotechnologies@see”, 3 April 2008, Workshop, 7 hours
o “... che Anova sior paron”, Dott. Massimo Borelli, 18 April 2008, Seminario, 2
hours
o “L‟analisi della varianza con il free software R”, Dott. Massimo Borelli, 15 May
2008, Seminary, 2 hours
o “physics@UniTs”, 19-20 June 2008, Meeting, 12 hours
o “Image Analysis and Biomechanical Modelling”, 1-2 September 2008,
Workshop, 6 hours
o “Modern Trends in Nanobiology and Nanomedicine”, 13-14 October 2008,
Workshop, 3 hours
o “IEEE Dresden 2008 NSS-MIC-RTSD”, 19-25 October 2008, Conference, 13
hours
o Congresso dei dottorandi di Nanotecnologie 2009 (14 hours)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
35
o “La statistica basta saperla oppure bisogna saperla faRe?”, Trieste, 21-23 Gennaio
2009, Lessons, Dott. Massimo Barelli, 9 hours
o “Introduction to Pore3D”, Basovizza, 23 February 2009, Talk, Dott. Francesco
Brun, 1 hour
o “Spatio-Temporal Radiation Biology: Transdiscipinary Advances for Biomedical
Applications”, Saint Feliu de Guixols (Spain),16-21 May 2009, 29 hours
o “Microbeam Radiation Theraphy (MRT) – Historical Prospective”, Saskatoon
(Canada), Dr. Daniel Slatkin, 27 August 2009, 1 hours
o “Joint Workshop of SFB762 & IMPRS”, Wittenberg (Germany), Workshop, 1011 September 2009, 7 hours
o How to write (and manage) a research project (5 hours)
o Congresso dei dottorandi di Nanotecnologie 2010 (14 hours)
Support educational activity and teaching
o Teaching temporary post at “Liceo Linguistico Pujati” (Sacile, PN), Math,
September-October 2008, 21 hours
o Teaching temporary post at “Liceo Linguistico Pujati” (Sacile, PN), Physics,
September-October 2008, 4 hours
o Participation as Tour Guide at the Canadian Light Source (July, 2009)
Other activities
o CT experiment at the SYRMEP beamline Elettra (Basovizza, TS, Italy), “Brain
distribution of nanogold targeted for brain delivery”, 12-14 October 2009
o CT experiment at the Photon Source (Chicago, Illinois, USA), “In situ bubble
nucleation and growth in crystal-bearing basaltic melts at 1 bar”, 11-13 November
2009.
o Analyzer Based Imaging experiment at the SYRMEP beamline Elettra
(Basovizza, TS, Italy) “Diffraction Enhanced Imaging of breast tissue samples
with the PICASSO detector”, 7-12 December 2009.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
36
Graduate Student: Francesca Bellomo
Title of the thesis: Biopolymer based scaffold for tissue engineering
Supervisor: Prof.Sergio Paoletti
Tutors (if any):Dott. Biasotto Matteo
Research Activity
The aim of my study is the development of biocomposite scaffolds for orthopedic applications,
based on the combination of polysaccharides (mainly alginate, Alg) engineered with bioactive
molecules and nano-hydroxyapatite (nHAp). In the first year of PhD, I have synthesized and
characterized the different components of the scaffold; furthermore I have prepared the composite
(Alg/Hap scaffolds) and studied their biological and structural properties. During the second year of
PhD, according the results obtained from the previous cytocompatibility tests, I have performed
preliminary in vivo tests. The animal model was New Zealand rabbit and the produced scaffolds
were inserted into bone defects on femur. Post-operatively, five fluorochromes were administered
sequentially every week. After 1 and 2 months, the rabbits were sacrificed and all the implanted
bones were analyzed using µ-CT and light and confocal microscopy. We observed a high level of
osteointegration of the scaffolds and ingrowth of newly deposited structured lamellar bone inside
them, indicating good osteoconductive properties. To introduce bioactive molecules and
antimicrobial agents, enhancing in this way the biological property of the scaffolds, we exploited
chitlac as vector. Chitlac is a lactitolated-derivative of chitosan that can be modified by chemical
grafting of bioactive peptides like RGD and that can be use to produce and stabilize silver
nanoparticles (nAg) with antimicrobial activity. By adsorbing the chitlac on the scaffold‟s surface
we spread the bio-signal inside the structure to induce a specific cell reaction. Alg/Hap scaffolds
were coated with chitlac-RGD and chitlac-nAg; on those, biological and antimicrobial in vivo tests
were performed. For both scaffolds we observed good cytocompatibility and, in the case of
scaffold with RGD, an improved cell proliferation; moreover, the scaffold with nAg showed a high
level of antimicrobial activity.
Objectives for the following year (if applicable)
The aims for the next year activity will be the in vivo tests on the bioactive scaffolds obtained, to
investigate both their integration and antimicrobial properties.
Publications on scientific journals (printed or in press)
Alginate/Hydroxyapatite biocomposite for bone ingrowth: a trabecular structure with high and
isotropic connectivity.Turco G, Marsich E, Bellomo F, Semeraro S, Donati I, Brun F, Grandolfo M,
Accardo A, Paoletti S. Biomacromolecules. 2009 Jun 8; 10(6):1575-83
Surface modification and polysaccharide deposition on BISGMA/TEGDMA based resins. Donati I.,
Travan A., Marsich E., Bellomo F., Achanta S., Semeraro S., Scarpa T., Toppazzini M., Spreafico
V., Paoletti S. Submitted to Biomacromolecules
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
37
Publications/abstracts in conferences/congresses (national or international)
ARACTERIZATION OF COMPOSITE POLYSACCHARIDE-BASED
SCAFFOLDS”
Gianluca Turco, Francesca Bellomo, Eleonora Marsich, Sabrina Semeraro, Ivan Donati,
Tommaso Scarpa, Andrea Travan, Francesco Brun, Agostino Accardo, Gianni Schena and
Sergio Paoletti (aprile 2008, Amsterdam; 7-9 novembre 2008 Bone-tec Hannover)
“POROUS
NANO-HYDROXYAPATITE/ALGINATE
SCAFFOLDS
FOR
BONE
REGENERATION” Eleonora Marsich, Sabrina Semeraro, Gianluca Turco, Francesca
Bellomo, Ivan Donati and Sergio Paoletti (7-9 novembre 2008 Bone-tec Hannover; Joint
Workshop of SFB762 & IMPRS 10th/11thSeptember 2009 in the Leucorea at Wittenberg)
“ EVALUATION OF CELLS ISOLATION AND BMPS PRESENCE ON DIFFERENT
CRYOPRESERVED BONE TISSUE” Francesca Bellomo, Matteo Biasotto, Michela
Faraon, Alessandro Viscioni, Maurizio Franco, Eleonora Marsich, Roberto Di Lenarda (7-9
novembre 2008 Bone-tec Hannover)
“SPERIMENTAZIONE IN VIVO DI SCAFFOLD DI ALGINATO E IDROSSIAPATITE PER
RIGENERAZIONE OSSEA” F. Bellomo, M. Biasotto, G.Turco, R. Di Lenarda, S. Paoletti
(22° riunione nazionale “A. Castellani” dei dottorandi di ricerca in discipline biochimiche
Brallo di Pregola (PV) 9 – 12 Giugno 2009; Collegio dei docenti di odontoiatria Roma 2224 aprile 2009)
Participation to conferences (as speaker)

Informal Workshop Scuola di Dottorato di Ricerca in Nanotecnologie and Int. Max-Planck
Research School for Science and Technology of Nanostructures in Halle on July 15-16,
2009
Educational Activity
Conferences, seminars, advanced courses and other didactic activities
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Confined and composite soft matter on the nanoscale (M. Saboungi, 11/04/08).
Scanning Probe Microscopies (SPMs), Agilent workshop al TASC (11 June 2008).
Modern Trends in Nanobiology and Nanomedicine (Workshop, 13-14 october).
La prevenzione nei laboratori di ricerca, seminario 12 marzo 2008, Trieste.
Seminario di scrittura scientifica ,7 maggio 2008 ( prof. Frederick Ruggeberg, Direttore del
Department of Oral Rehabilitation del Medical College of Georgia ).
Wednesday 16th april at 10.30 in the TASC seminar room, there will be
held the phd students briefing: C. Blasetti, E. Sovernigo.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
38
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AFM Workshop organized by SITEM / Agilent Technologies, Trieste, June 11th.
Bone-tec , Hannover 2008.
Image Analysis and Biomechanical Modelling: From Biomolecules to Bioactive
Biomaterials (Trieste 1st-2nd September 2008).
Phd Course Biotecnologie cellulari e Molecolari applicate alla medicina “Molecular
Biotechnologies applied to Medicine” Santa Margherita Ligure (Genova) 20-22 February
2008.
“Bilateral Workshop on Biopolymers” TRD (NTNU) - TRS (UNITS) Trieste, October 1st3rd, 2009.
Workshop @ Elettra"CCD per Imaging e Spettroscopia" 28 Gennaio 2009 Elettra Sincrotrone Trieste.
“Modern Trends in Nanobiology and Nanomedicine” October, Monday 13th and Tuesday
14th - Basovizza – Trieste.
22° riunione nazionale “A. Castellani” dei dottorandi di ricerca in discipline biochimiche
Brallo di Pregola (PV) 9 – 12 Giugno 2009.
Seminars of reology, Romano Lapasin, DICAMP-University of Trieste, 20-22 July 2009.
Joint Workshop of SFB762 & IMPRS 10th/11thSeptember 2009 in the Leucoreaat
Wittenberg .
“How to write (and manage) a research project” 16 October 2009 –University of Trieste.
“St-scCO2 sterilization by means of supercritical Carbon Dioxide” international workshop,
University of Trieste, 10th February 2009.
“Incontri informativi per i dottorandi” di ARABITO STEFANIA - lunedì, 22 giugno 2009,
Trieste ,Italia.
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Support educational activity and teaching
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Laboratory tutor within LLC-Trieste (Life Learning Center), hands-on laboratory
activities in the field of Life Sciences for secondary schools students and teachers.
Teaching assignment, “Laboratory of Biochemistry II”, degree in Medical
Biotechnology, School of Medicine, University of Trieste.
December 2009: Secondary school teachers‟ training. Project “LLC-Trieste on the
territory”, at the secondary school “N. Tron” of Schio (VI, Italy).
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
39
Graduate Student GIOVANNI BIRARDA
Title of the thesis: Development of microfluidic devices for biomedical applications of
Synchrotron Radiation Fourier Transform Infrared Microspectroscopy
Supervisor: Prof. Alfonso Franciosi
Tutors: Dott.ssa Lisa Vaccari – Dott. Luca Businaro
Research Activity
This PhD project focuses on the development of microfluidic devices transparent to both visible and
mid-infrared (MIR) light, in order to study the biochemical response of living cells to diverse
stimuli, by exploiting capabilities of FTIR microspectroscopy (μ-FTIR) with synchrotron radiation
(SR).
During this second year, the manufactured 2D prototypes designed, fabricated and tested during the
first year of the PhD, have been used to investigate the biochemical response of leucocytes to both
mechanical and /or chemical stimuli. Living cells belonging to two different cell lines (THP1 and
U937) have been measured in vitro in diverse conditions: in resting state, undergoing purely
mechanical deformation, subjected only to chemical activation (activating agents: fMLP -formylMethionyl - leucyl-Phenylalanine- and PMA -Phorbol-12-Myristate-13-acetate-), and combaining
the two sources of stimulation.
Contemporary, we further optimized the fabrication processes for producing a three- dimensional
microfluidic device and the engineering of a home-made thermalized sample holder with
connections for syringe pumps.
The new prototype will be used during the beamtime scheduled for 14-19 December 2009 at he
infrared beamline of Elettra Sincrotrone Trieste (SISSI). Primary human leukocytes (monocytes and
lymphocytes) will be measured trying to validate the data collected so far using cells belonging to
the same cellular phase (synchronized cells).
Objectives for the following year
o Move from static experiments to dynamic ones by using 3D microfluidic devices
transparent both to MIR and visible.
o Measure primary cells instead of immortalized ones.
o Optimize algorithms for the water subtraction and for the correction of spectral
artefacts due to Mie scattering phenomena in order to improve data analysis
Publications on scientific journals (printed or in press)
oGiovanni Birarda, Gianluca Grenci, Luca Businaro, Benedetta Marmiroli, Sabrina
Pacor, Lisa Vaccari - “Fabrication of a microfluidic platform for investigating
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
40
dynamic biochemical processes in living samples by FTIR microspectroscopy –
Microelectronic Engineering (in press)
oGiovanni Birarda, Gianluca Grenci, Luca Businaro, Benedetta Marmiroli, Sabrina
Pacor, Federica, Piccirilli, Lisa Vaccari - Infrared microspectroscopy of
biochemical response of living cells in microfabricated devices” – Vibrational
Spectroscopy (submitted - evaluated) 2009
Publications/abstracts in conferences/congresses (national or international)
oPoster Presentation in the 5th International Conference on Advanced
Vibrational Spectroscopy, Melbourne (Australia) from 12-17 July 2009
oPoster Presentation in the 2nd PhD Workshop Wittenberg 10-11 September
2009
oPoster Presentation in the 35th International Conference on Micro & Nano
Engineering, Ghent (Belgium) from 28 September - 1 October 2009.
oPoster Presentation in the 13th International Conference on Miniaturized
Systems for Chemistry and Life Sciences (MicroTAS 2009), Jeju (South
Korea) from 1-5 November 2009.
oThe Proceedings of Conference on Miniaturized Systems for Chemistry and
Life Sciences (MicroTAS 2009), Giovanni Birarda,Gianluca Grenci, Luca
Businaro, Benedetta Marmiroli, Giuseppe Bellisola and Lisa Vaccari, Single
Living Cell Biochemical Rearrangements Investigated By Infrared
Microspectroscopy In Microfluidic Devices - 978-0-9798064-21/μTAS2009/$20ⓒ2009CBMS pp 338-341.
Participation to conferences (as speaker)
oFabrication of microfluidic platform for infrared microspectroscopy analysis
of dynamic biochemical processes in living sample. NNC2009. Pavia, 21-22
September 2009
Educational Activity
Conferences, seminars, advanced courses and other didactic activities
o Micelles, your cells", by Michael Rappolt;11 February 2009 1 h
o CDK7 and CDK9 at the interface of Cell Cycle and Transcription Regulation: from
structure to function; Graziano Lolli; 4 May 2009; 1 h
o Advanced Imaging and Spectromicroscopy methods for chemical and structural
characterization of micro- and nano-materials; 11-15 May 2009; Dott.sa Maya
Kiskinova; 15 h
o Joint Workshop between the SFB762, the Nano-IMPRS and the GRADUATE
SCHOOL OF NANOTECHNOLOGY; September 2009; Wittenberg; 15 h
o NNC2009. Pavia, 21-22 September 2009; 12 h
o 13th International Conference on Miniaturized Systems for Chemistry and Life
Sciences (MicroTAS 2009), Jeju (South Korea) from 1-5 November 2009, 30 h
 Support educational activity and teaching:
o Laboratory demonstration in clean room for students of Material Science of
Padova University (Prof. Filippo Romanato), 6 h 29th of may 2009
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
41
Graduate Student: LUCAFONTANIVE
Title of the thesis: Nanoscale interaction for higher efficiency of contrast media
Supervisor: Prof. Attilio Cesàro
Tutor: Dr. Nicola D’Amelio
Research Activity
In this study we investigate the nanoscale interactions in solution of iodurated contrast media of
oversaturated solutions to control and to tune the aggregation phenomenon. During this year NMR
spectroscopy was mainly used. Experiments were carried out to evaluate the size and mobility of
aggregates from diffusion and relaxation time. The ratio T1/T2 gives information about local and
global mobility to identify the moieties involved in intermolecular interactions. Chemical shift
deviations as a function of concentration confirms the molecular regions involved in interactions.
Few molecules seem to be involved into a nanostructurated aggregate. Aggregations seems to
involve mainly lateral chain of the ICM. To check hydrogen bonds in solution from amidic protons
chemical shift deviation data were carried out as a function of temperature, but stable hydrogen
bonds were not revealed.
In addition, ICM solutions were studied by raman spectroscopy in function of concentration at
constant temperature in university of Perugia. Changes in conformation were not revealed.
However, an anomalous peak was observed at low frequencies (20 cm-1) for iomeprol, iopamidol
and iopromide. This could be due to rotation around single bond between aromatic system and
carbonilic groups.
Objectives for the following year (if applicable)
The objective is to identify all the accessible conformations in solutions by using 1H and 13C
spectra of simple molecules of ioduinated contrast media; the aim is to know the percentages of
atropisomers in solutiona at high concentrations to control aggregation phenomenon. Other
techniques in solution (scattering Raman) and on the solid state (calorimetry) will be
complementary used. The final aim is to understand and to tune the phenomenon of the
atropisomerism that is responsible of nanostructurated aggregates in solutions.
Publications on scientific journals
D‟Amelio N, Fontanive L, Uggeri F, Suggi-Liverani F, Navarini L. NMR reinvestigation of the
caffeine-chlorogenate complex in acqueous solution and in coffee brews. Food Biophys. 2009, 4,
321-330.
Educational Activity
Conferences, seminars, advanced courses and other didactic activities
Young Researchers Oral Communication, EuroNanoMedicine 2009, Bled, Slovenia,
September 28 – 30
Seminario sul Virtual Private Network (VPN), Prof. Maurizio Fermeglia, 20 Marzo
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
42
Modeling reaction at the nanoparicle scale, Prof. Phillip R. Westmoreland, Dept of
Chemical Engineering, University of Massachusetts Amherst, 08 Maggio
Chimica fisica delle interfasi in sistemi nano strutturati, Dr. Italo Colombo, 15 Maggio
Strategie bioprotettive in sistem alimentari: il modello dei tessuti vegetali, Prof. ssa Paola
Pittia, Dipartimento di Scienze degli Alimenti, Università degli studi di Teramo, 28
Maggio
Strategie adattative in invertebrati estremo fili: il modello animale tardigradi, Prof.ssa
Lorena Rebecchi, Dipartimento di Biologia Animale, Università di Modena e Reggio
Emilia, 28 Maggio
How to write (and manage) a research project, Prof. Valter Sergo, Prof. Maurizio
Fermeglia, Dr. Cecilia Blasetti, 16 Ottobre
SPETTROSCOPIA RAMAN: Dott. M. Paolantoni, Dipartimento di Chimica, Università
di Perugia. (Settembre 2009, 6 h)
SPETTROSCOPIA NMR: Dott. Nicola D‟Amelio, Bracco Imaging S.p.A. - CRB TS
(Febbraio 2009, 20 h)
CALORIMETRIA: Dott. F. Sussich (Febbraio 2009, 6 ore)
Support educational activity and teaching
Training of a polish student (S. DEJA Univ. Wroclaw) on theoretical bases of NMR spectroscopy.
Area di Ricerca Science Park (July 2009, 20 h).
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
43
Graduate Student Giulio Marchesi
Title of the thesis: Analysis of stability of bonded interfaces in dentistry: the role of MMps and
low contraction materials.
Supervisor: Prof. Lorenzo Breschi
Tutors: Prof.ssa Milena Cadenaro
Research Activity
Despite excellent immediate and short-term bonding effectiveness of dental adhesives, the
durability and stability of resin bonded interfaces on dentin created by some bonding systems
remains questionable. The research activity of the present year investigated various factors involved
in the failure of bonded interfaces:
1.insufficient impregnation of the dentin substrate;
2.increased permeability of hybrid layer;
3.degradation of dentinal collagen fibrils due to endogenous dentin MMPs.
These phenomena are influenced by the elastic modulus of the adhesive system, thus the percentage
of residual ethanol on E-modulus of experimental adhesive blends was investigated. The results of
this study showed that resin blends with 10% 20% residual ethanol produced a significant reduction
in the E-modulus after 24h water storage.
Another experimental protocol investigated bond strength and stability of the adhesive interface of
different self adhesive cements used to lute fiber posts within intraradicular dentin after storage in
artificial saliva for 1 year. The value of the different self adhesive cements never exceeded 10 MPa
of bond strength and degradation of the adhesive interface between dentin and cements was
investigated. Since previous studies demonstrated a protective role of chlorexidine in inhibiting the
bond degradation induced by MMPs, an experiment was carried out to test the influence of 2%
digluconate chlorexidine as additional primer during the luting procedure with self adhesive on
fiber posts. The results have shown no differences between experimental groups and control groups
at 6 months. For the final result we are looking forward to investigate the 1yr specimens currently
ongoing.
Objectives for the following year (if applicable)
A research protocol an experiment to evaluate the release of chlorexidine from dentin bonding
systems after mechanical cycling will be performated. Resin disks with known composition of
monomer (at increasing hydrophilicity) containing chlorhexidine in different concentrations were
polymerized, and analyzed it with spectroscopy after cyclic loading. Since the peak of release of
CHX is overlapped by the peak of methacrylate resin we are currently looking for a new method to
investigate the content of the eluates (HPLC) .
Publications on scientific journals (printed or in press)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Cadenaro M, Marchesi G, Antoniolli F, Davidson CL, De Stefano Dorigo E, Breschi L.
“Flowability of composites is no guarantee for contraction stress reduction.” Dental
Materials 2009;25(5):649-54.
Mazzoni A, Marchesi G, Cadenaro M, Mazzotti G, Di Lenarda R, Ferrari M, Breschi M.
“Push-out stress for fiber posts luted using different adhesive strategies.” European Journal
Oral Science 2009;117(4):447-453.
Cadenaro M, Pashley DH, Marchesi G, Carrihlo M, Antoniolli F, Mazzoni A, Tay FR, Di
Lenarda R, Breschi L. “Influence of chlorexidine on the degree of conversion and Emodulus of experimental adhesive blends”. Dental Materials 2009;25(5):1269-74.
Marchesi G, Breschi L, Antoniolli F, De Stefano Dorigo E, Ferracane J, Cadenaro M.
“Contraction stress of composite materials with different polymerization reactions assayed
by low-and high-compliance systems. Dental Materials submitted.
Publications/abstracts in conferences/congresses (national or international)
Marchesi G, Cadenaro M, Antoniolli F, Di Lenarda R, Pashley DH, Breschi L. “Influenza della
clorexidina sul grado di conversione e sul modulo elastico in adesivi sperimentali.” Acts of
XIII national meeting SIDOC-Rome 19-21/2/2009.
Marchesi G, Breschi L, Antoniolli F, De Stefano Dorigo E, Cadenaro M. “Stress da
polimerizzazione di una resina a base siloranica.”Acts of national meeting Docenti di
Odontoiatria e di chirurgia Maxillofacciale, Roma 22-24/4/2009.
Marchesi G, Cadenaro M, Breschi L, Antoniolli F, Tay FR, Di Lenarda R, Pashley DH.
“Influence of chlorexidine on the E-modulus of experimental resins.” J Dent Res 88 (spec
Iss A). Annual meeting of The International Association for Dental Research. Miami FL
USA.
Marchesi G, Cadenaro M, Di Lenarda R, Breschi. Bond strenght of Post Luted with different
Self-adhesive Resin Cements. Annual meeting of the Accademy Dental Materials. Portland
Oregon USA 29-31/10/2009.
Other publications
Breschi L, Cadenaro M, Mazzoni A, Navarra CO, Marchesi G, Di Lenarda R, De Stefano
Dorigo E. “La durata del legame adesivo:la nuova sfida dell‟ odontoiatria resteurativa.”
Dentista Moderno 2009;4:41-54.
Marchesi G. Analysis of aging and stability of bonded interface in dentistry. Poster 2°
Workshop of the SFB 762-Wittenberg, Germany 10-11/09/2009.
Educational Activity
Periods abroad (date and place)
Meeting research Department of Oral Biology, School of Dentistry Medical College of Georgia,
Augusta Georgia, USA 30-31/4/2009.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
45
Conferences, seminars, advanced courses and other didactic activities
National meeting of Sidoc Roma 2009 19-21/2/ 2009.
Annual meeting IADR Miami Usa 1-4 aprile 2009. Guided tissue remineralization prevents invitro degradation of resin-dentin bonds Tay Fr. , Recents advences in dental monomero and
polymerization processes. Spencer P, Ferracane J.L. ; Tem interfacial characterization of an
adhesive-free composite to enamel dentin. Van Meerbeek B.
Training course “ Odontoiatria dell‟ età evolutiva” Trieste 16-17/1/2009 20-21/1/2009 2223/5/2009.
Seminar of ARPE for VII° PQ Trieste 16/09/2009.
Seminar “How to write a research project” Trieste 16/10/2009.
Support educational activity and teaching
“Traumatology in pedodontic” course of Pedodontics Prof.Cadenaro IV° year Corso di Laurea
Specialistica in Odontoiatria e Protesi Dentaria, University of Trieste.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Graduate Student ANTONIO RAMPINO
Title of the thesis: Polysaccharide based nanoparticles for drug delivery
Supervisor: Prof. Cesàro Attilio
Tutors (if any): Dr. Borgogna Massimiliano, Dr. Bellich Barbara
Research Activity
During my PhD period the purpose is to develop and characterize biodegradable polymeric
nanoparticles as drug carriers as well as to promote the rational and optimal design and
development of novel carrier systems. More specifically, at the end of my project likely I will be
able to design original nanocarriers and to have fully characterized the systems in order to tailor
specific properties. This includes the tuning the molecular interactions in the polymer assemblies at
nanoscale level.
During my second year, the production of chitosan carriers based on ionotropic nano-hydrogels
formation induced by tri-(poly)-phosphate (TPP) has been optimized. We focused our attention on
several aspects, such as physical-chemical properties, yield, storage, production automatization, etc.
To optimize chitosan properties and solve pharmaceutical-technological problems we used two
different classes of molecularly engineered chitosan derivatives: a trimethylated chitosan and
glycosil-based derivatives.
In order to investigate drug loading capacity different model drugs has been used: OVA, BSA,
Human Insulin, Bovine Insulin.
We investigated the association of Chitosan carriers with other biopolymers: Alginate and different
Pectins. Two different methods have been used: nanoparticle coating and polyelectrolyte
complexation.
Finally, a collaboration has been started with the University of Innsbruck for the mucoadhesivity
study and with the University of Bremen for the a deep investigation of the dimensional properties
of our polymers as a function of the chemical derivatization.
Objectives for the following year (if applicable)
The aims of my third PhD year are:
 To introduce different engineered chitosan derivatives
 To optimize nanocarrier coating using different biopolymers: alginate and pectin
 To investigate nanoparticle formation using the polyelectrolyte complexation
 To focus our attention on human insulin encapsulation and characterization of loaded
carriers (drug release, protection effect, etc.)
 To extend the investigation about drug loading using different types of model
peptide/protein drugs
 In vivo ed ex vivo studies
 Characterization of produced systems ( nanometric resolution spectroscopies, physicochemical properties, ect.)
Publications on scientific journals (printed or in press)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Publications/abstracts in conferences/congresses (national or international)
EUCHIS 2009, 9th International Conference of the European Chitin Society, Venice, Italy, 2326 May 2009
Participation to conferences (as speaker)
Young Researchers Oral Communication, EuroNanoMedicine 2009, Bled, Slovenia, 28-30
September 2009
Educational Activity
Periods abroad (date and place)
NBP Tour 2009, presso Materials and Science Group dell‟ Istitut Quimic de Sarrià (IQS),
Barcellona, Spagna, 29/06 – 03/07
Classes followed (date, course, professor, type of course)
Conferences, seminars, advanced courses and other didactic activities
Seminario sul Virtual Private Network (VPN), Prof. M. Fermeglia, 20/03
Modeling reaction at the nanoparicle scale, Prof. P. R. Westmoreland, Dept of Chemical
Engineering, University of Massachusetts Amherst, 08/05
Chimica fisica delle interfasi in sistemi nano strutturati, Dr. I. Colombo
Strategie bioprotettive in sistem alimentari: il modello dei tessuti vegetali, Prof. ssa P. Pittia,
Dipartimento di Scienze degli Alimenti, Università degli studi di Teramo, 28/05
Strategie adattative in invertebrati estremofili: il modello animale tardigradi, Prof.ssa L.
Rebecchi, Dipartimento di Biologia Animale, Università di Modena e Reggio Emilia, 28/05
How to write (and manage) a research project, Prof. V. Sergo, Prof. M. Fermeglia, Dr. C.
Blasetti, 16/10
Fracture physics of biopolymer gels, Prof. T. Baumberger, Institut des NanoSciences de Paris,
France, 20/10
Corso di Reologia, Prof. R. Lapasin, DICAMP, Università di Trieste, 07/2009,
Corso Avanzato di Calorimetria, Dott.ssa F. Sussich (6 h)
Support educational activity and teaching
Trainig of a Polish visiting student (Mateusz Gosecki, University of Lodz, Poland) on drug
delivery, ionotropic nano-hydrogels formation and polymer characterization. 06/2009 (20 h)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
48
Graduate Student XENJA SANTARELLI
Title of the thesis: Correlation between structure and reactivity for nanostructured catalysts
Supervisor: prof. Renzo Rosei
Tutors (if any):
Research Activity
The aim of this thesis is to highlight correlations between the activity of nanostructured catalysts
and their structural and compositional characteristics.
Catalytic properties in metallic alloys are often related to those of their components in a
nonintuitive way.Several catalyst parameters (not only the geometrical ones) seem to modulate
reactivity. Determining if and how these effects compete or act together is the objective.
Preliminary DFT calculations on several bimetallic alloys, made by members of our group, suggest
what are the best candidates as catalysts for selected reactions (WGS, PROX).
After a check of real samples‟ activity, their careful structural characterization is required.
I dedicated the first two years of my studies how to learning how to analyse data collected with
EXAFS spectroscopy and TEM, and now I‟m involved with the MCX beamline (Elettra - powder
diffraction) in characterizing nanostructured catalyst samples produced in the framework of an
international collaboration. This beamline is equipped with an oven to bring the samples to 1200C,
to observe structural changes related to temperature variations. The last months we have been
occupied in tweaking and interfacing it with the data analysis system (Labview). Both the oven and
the data acquisition system are currently operating. During a recently concluded experimental
session we have analyzed catalysts samples that have been already subjected to Electron
Microscopy, to identify structural variations that can appear in inert atmosphere as a consequence of
the annealing process. The data analysis will be concluded in a short time.
Objectives for the following year (if applicable)
Several problems that arose during measurements highlighted modifications that must be
implemented in the oven and in the samples. Most of them are related to the labor intensive
alignment phase. Moreover, the first results seem to suggest that it‟s necessary to complement the
XRD technique with EXAFS. Concerning the samples, it may be necessary to change the sample
support (alumina) to a new one that doesn‟t obscure NC‟s signal.
In the next months it will be possible to make new measurements at the MCX beamline with the
oven and a reactor to test the samples while a PROX reaction goes on. The possibility to make RT
measurements with the high resolution diffractomenter will also be evaluated.
Other publications
“Dependence of the fractional anisotropy in cervical spine from the number of diffusion gradients,
repeated acquisition and voxel size”, Santarelli, X., Garbin, G., Ukmar, M., Longo, R.; Magnetic
Resonance Imaging (in press)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
49
Educational Activity
Classes followed (date, course, professor, type of course)
50h - laboratory, partially spent at the MCX beamline and partially dedicated on learning data
analysis software (Origin, FIT2D)
Conferences, seminars, advanced courses and other didactic activities
13-15.1.09: 13.5h (Congress of the School)
20.3.09: 1h seminar (prof. Fermeglia, VPN e Portale)
8.5.09: 1h seminar (prof. Phillip R. Westmoreland,
Modeling reactions at the nanoparticle scale)
30.6.09: 3h corso (Arabito Stefania,
Incontro informativi per i dottorandi di area scientifica)
24.6.09: 2h (Loredana Casalis,
Trasporto elettronico nei SAM; Applicazioni in campo
biologico)
26.6.09: 2h (Alberto Morgante, Determinazione di strutture di SAM di alcantioli
su superfici di Au. Tecniche strutturali)
29.06.09: 2h (Alberto Morgante,
Film autoassemblati di aminoacidi su superfici metalliche.
Determinazione delle stato chimico e del tipo di legame
con tecniche
spettroscopiche di fotoemissione ed assorbimento di raggi X)
01.07.09: 2h (Lucia Pasquato,
Nanoparticelle, colloidi.
Metodologie di sintesi di nanoparticelle protette da un
monostrato organico. Tecniche di caratterizzazione.
Packing del monostrato).
03.07.09: 2h (Lucia Pasquato,
Metodi di sintesi di nanoparticelle funzionalizzate.
Monostrati misti. Nanoparticelle monovalenti e divalenti).
16.10.09: 5h Seminar (Blasetti-Sergo-Fermeglia,
How to write and manage a research project)
7-18.09.09: 50 h X School of Synchrotron Radiation: fundamentals, methods and
applications (Duino-Trieste)
26.11.09: 1h CENMAT presentation
Support educational activity and teaching
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
50
Graduate Student ENRICO SOVERNIGO
Title of the thesis: Influence of nanostructure on the electronic properties of heterojunctions
in photovoltaic cells.
Supervisor: Prof. Alberto Morgante
Tutors (if any): Dott. Massimo Tormen
Research Activity
Photovoltaic solar cells based on organic materials show an high absorption of light and conversion
to electric charges, however, thay have scarce properties of transport the photo-generated charges.
Thus, the study of innovative conctacts‟ geometries has been developed to better explicate the great
potential of this kind of cells. In particular we want to realize a layout in which the ligth and current
path are orthogonal to each other. For this purpose, in this year the research activity has been
pointed to the project and assembling of a suitable high vacuum system for the evaporation of both
metals and organics at variable incidence angles. Moreover, this system has to be coupled to a glove
box in inert atmosphere in order to perform more processes avoiding contaminations and
degradations od materials.
In the meantime, the X-ray lithographic technique has been studied at the synchrotron beamline
LILit1 getting, by writing a computational code for the optics simulation, to the comprehension of
this high resolution exposition system. This technique will be used to fabricate substrates for the
cells mentioned above.
In former studies, light trapping devices based on microlenses were developed. Thus, a deposition
protocol for transparent conductive electrodes has been optimized in order to enhance the
applications of this system.
In conclusion, we succeded in appling for a beamtime on ALOISA 2 beamline in order to carry on
some more experiment on charge transfer in organic systems for photovoltaic.
Objectives for the following year (if applicable)
o Fabrication of photovoltaic cells with a nano-series interconnection;
o Charge transfer experiments in organic compounds for photovoltaics;
o Electronic characterization system for organic semiconductors (Tutor for a master student).
Other publications
Poster at the Joint Workshop of PhD students, 10-11 september 2009, Wittemberg Lutherstadt,
Germany.
Participation to conferences (as speaker)
1
2
LILit Laboratorio Interdisciplinare di Litografia presso Sincrotrone Elettra, Trieste.
ALOISA linea di luce multifunzionale per la scienza delle superfici presso Sincrotrone Elettra, Trieste.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
51
Phd Briefing TASC “Design and realisation of a thermal evaporation system for organic solar cells
with special geometry” 23 july 2009, Trieste.
Educational Activity
Periods abroad (date and place)
Classes followed (date, course, professor, type of course)
1-7 july 2009 Synthesis methods for colloidal nanoparticles, L. Pasquato, PhD Nanotechnology.
Conferences, seminars, advanced courses and other didactic activities
o
o
o
o
o
o
o
o
o
o
o
o
o
Seminar H. Pakdast Technical University of Denmark, 26 January 2009, Trieste.
Seminar A. Tredicucci, 31 March 2009, Trieste.
Seminar I. Colombo “Chimica fisica delle interfasi in sistemi nanostrutturati”, 15 May 2009,
Trieste.
Seminar Prof. P. R. Westmoreland University of Massachusetts Amherst, 8 May 2009, Trieste.
Seminar Chris Armstrong “Photovoltaic Measurements: Testing the Electrical Properties of
Today´s Solar Cells” 27 May 2009.
Seminar D. Sammito "Dispositivi Elettroluminescenti in Silicon Rich Oxide Drogato con
Erbio", 18 June 2009, Trieste.
International PhD summer school on Organic Photovoltaics ISOPHOS09, 13-15 July 2009,
Valencia, Spagna.
Seminar E. D‟Este, “High spatial resolution BDNF delivery by means of laser tweezers to
stimulate hypocampal neurons signalling” 23 July 2009, Trieste.
Joint Workshop of PhD students, 10-11 September 2009, Wittemberg Lutherstadt, Germany.
Seminar G. Kladink "Ultra fast charge transport in nanotubular alpha-MnO2 self assembly
studied by X-ray resonant photoemission" 17 September 2009, Trieste.
24th European Photovoltaic Solar Energy Conference, 21-25 September 2009, Hamburg,
Germany.
Seminar How to write (and manage) a research project, 16 october 2009, Trieste.
Seminar V. Lanzilotto:"Thin organic films and their charge transfer properties: an outlook on
single molecule devices.", 16 October 2009, Trieste.
Support educational activity and teaching
Tutor for a master thesis on Material Science: “Preparazione, caratterizzazione ed ottimizzazione di
film sottili di ossido di indio-stagno per celle fotovoltaiche”, Fabio Morpurgo, Università di
Padova.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
52
Dottorandi del 24 ciclo: •
Graduate Student MATTEO CARGNELLO – email [email protected]
Laboratorio di lavoro: lab. 439-523 Dip. Sc. Chimiche, Edificio C11
Title of the thesis: Rare-earths oxides-based nanostructures as heterogeneous catalysts
Supervisor: Prof. Paolo Fornasiero
Tutors (if any): Dr. Tiziano Montini
Research Activity
The research activity mainly consisted in the development of an approach to obtain stable
materials for heterogeneous catalytic reactions. The methodology is based on the encapsulation of
preformed metal nanoparticles inside porous oxides in order to limit the sintering of the metal phase
under reaction conditions and therefore to avoid the deactivation of the catalysts.
We initially focused our attention to Au, Pd and Cu embedded inside ceria (CeO2).
1) Au has been encapsulated inside ceria through a supramolecular approach which addresses
the preferential precipitation of Ce(OH)x around pre-formed Au nanoparticles. The materials
obtained showed very good activities for the PReferential OXidation of CO in the presence of
excess H2 (PROX) and remarkable stability after simulated aging treatments. Moreover, the
developed catalysts showed better activities than those obtained with Au/CeO2 materials prepared
through optimized literature methods. The characterization with complimentary techniques such as
X-ray Absorption Spectroscopy, Scanning-Transmission Electron Microscopy and chemisorption
confirmed the formation of small Au nanoparticles protected by a layer of porous oxide.
2) Pd@CeO2-type systems has been realized by self-assembly in solution of the components
(thiolate-protected Pd nanoparticles and Ce alkoxides). The obtained core-shell structures have been
adequately functionalized to make them dispersible in organic solvents; the structures have been
characterized and successfully used as flexible precursors in the preparation of active catalysts for
the Water-Gas Shift-Reaction (WGSR).
3) Cu@CeO2-type catalysts have been prepared using water-in-oil microemulsions. Cu
nanoparticles have been prepared inside the water droplets of the microemulsion and then a cerium
alkoxide has been hydrolyzed to give the final Cu@CeO2 structures. Despite the low Cu loading
(2.5 wt. %), the materials resulted very active for both PROX and WGSR. The stability of the
catalysts is very promising, with no loss of activity under PROX condition even after aging at 550
°C for 12 hours (harsh conditions used to simulate an accelerate aging).
The results are encouraging as they highlight the possibility to use the embedding approach for the
obtainment of highly active and stable catalysts for different reactions and with different metalmetal oxide combinations.
Objectives for the following year (if applicable)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
53
The main objectives are the extension of the embedding approach to other metals and metal oxides
combinations and the deep characterization of the materials already prepared.
Publications on scientific journals (printed or in press)
1) V. Gombac, L. Sordelli, T. Montini, J. J. Delgado, A. Adamski, G. Adami, M. Cargnello, S.
Bernal and P. Fornasiero, “CuOx-TiO2 Photocatalysts for H2 Production from Ethanol and Glycerol
Solutions.”, The Journal of Physical Chemistry A, in press, doi:10.1021/jp907242q.
2) L. De Rogatis, M. Cargnello, V. Gombac, B. Lorenzut, T. Montini and P. Fornasiero,
“Embedded phases: a way to active and stable catalysts.”, ChemSusChem, in press.
3) M. Cargnello, T. Montini, S. Polizzi, N. L. Wieder, R. J. Gorte, M. Graziani and P. Fornasiero,
“Novel embedded Pd@CeO2 catalysts: a way to active and stable catalysts.”, Dalton Transactions,
in press.
Other publications
1) M. Cargnello, N. L. Wieder, T. Montini, R. J. Gorte and P. Fornasiero, “Synthesis of dispersible
Pd@CeO2 core-shell nanostructures by self-assembly”, Journal of the American Chemical Society,
submitted.
Participation to conferences (as speaker)
- “Metal-doped TiO2 for hydrogen production”, V. Gombac, T. Montini, M. Cargnello, M. Graziani
and P. Fornasiero, Slovenian Conference on Materials and Technologies for Sustainable Growth,
oral presentation, University of Nova Gorica, Ajdovscina, Slovenia, 12th May 2009
- Informal workshop Scuola di Dottorato di Ricerca in Nanotecnologie and Int. Max-Planck
Research School for Science and Technology of Nanostructures, oral presentation “Rare-earths
oxides-based nanostructures as heterogeneous catalysts”, Max Planck Institute of Microstructure
Physics, Halle, 15th-16th July 2009
- “I giovani e la chimica in Friuli-Venezia Giulia”, oral presentation “Self-assembled materials
based on metallic nanoparticles for heterogeneous catalysis”, Department of Chemistry, University
of Trieste, 24th September 2009
- “Active and stable Au catalysts for H2 purification”, T. Montini, M. Cargnello, C. Gentilini, L.
Pasquato, P. Fornasiero, S. Mehraeen, N. D. Browning, E. Fonda, GOLD 2009, 5th international
conference on gold technology and applications, Heidelberg (Germany), poster presentation, 25th30th July 2009
- 2nd PhD workshop, Martin-Luther Universitat Halle-Wittenberg, Wittenberg, Germany, poster
presentation “Active and stable Au catalysts for H2 purification”, 10th-11th September 2009
Participation to conferences (as co-author)
- “Photocatalytic H2 production over optimized CuOx-TiO2 nanocomposites.”, V. Gombac, T.
Montini, L. Sordelli, M. Cargnello, M. Graziani, P. Fornasiero, Summer School in catalysis 2009,
Porto Carras, Greece, May 2009 (poster presentation by V. Gombac)
- “Design of active and stable catalysts for H2 purification.”, T. Montini, M. Cargnello, C. Gentilini,
L. Pasquato, P. Fornasiero, S. Mehraeen, N. D. Browning, E. Fonda, E-MRS 2009 Spring Meeting,
Strasbourg, June 2009 (oral presentation by T. Montini)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
54
- “Photocatalytic H2 production over optimized CuOx-TiO2 nanocomposites.”, P. Fornasiero, V.
Gombac, T. Montini, L. Sordelli, M. Cargnello, M. Graziani, VII Convegno Nazionale Consorzio
Scienza e Tecnologia dei Materiali, Tirrenia, Italy, June 2009 (oral presentation by P. Fornasiero)
Educational Activity
Periods abroad (date and place)
- Research center ICCOM-CNR Florence, Sesto Fiorentino, AFM experiments, 1-7 February 2009
- Sinchrotron Soleil, Gif-sur-Yvette, Paris, EXAFS experiments, 19-23 February 2009
- Indian Institute of Technology, Chennai, 31 March – 5 April 2009
Classes followed (date, course, professor, type of course)
- “Tecniche di caratterizzazione con luce di sincrotrone”, Prof. G. Vlaic, corso di laurea magistrale
in chimica, 40 hours
- “Proprietà fisiche dei materiali”, Prof. A. Franciosi, corso di laurea magistrale in chimica, 40
hours
- Course “Molecular self-assembling and nanostructures”, Dr. L. Casalis, Prof. A. Morgante, Prof.
L. Pasquato, 8 hours
- Course “Advanced Microscopy”, Dr. L. Felisari, Dr. C. Africh, 14 hours
- Course “Photoemission Spectroscopies”, Prof. A. Baraldi, 11 hours
Conferences, seminars, advanced courses and other didactic activities
- Annual meeting PhD School in Nanotechnology, 13-15 January 2009, total 20 hours
- “Information meetings for doctoral students”, 30 June 2009, 3 hours
- “Seminar on writing (and managing) a research project”, 16th October 2009, 4 hours
- Annual meeting PhD School in Chemical and Pharmaceutical Sciences and Technologies, 21-23
January 2009, total 10 hours
- 1st International Conference on Nanustructured Materials, Kottayam, Kerala (India), 6-8 April
2009
- Conference “Modeling reactions at the nanoparticle scale”, Prof. P. Westmoreland, 8 May 2009
- Conference “Chimica fisica delle interfasi in sistemi nanostrutturati”, Dr. I. Colombo, 15 May
2009
- Conference “Supramolecular Chemistry of Naphthalenediimides”, Dr. G. Dan Pantos, 8 June 2009
- Conference “New chemistry of dithiolenes: from biomimetic models to sensor”, Prof. Partha Basu,
13 July 2009
- Conference “NMR Techniques for Investigating the Supramolecular Structure in Solution”, Prof.
Alceo Macchioni, 17 September 2009
- 3rd year PhD students presentations, 26 November 2009
Support educational activity and teaching
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
55
Graduate Student:
LUCA COZZARINI
Title of the thesis: “Nanostructured materials for photovoltaic applications”
Supervisor:
Prof. Valter Sergo
Tutor:
Prof. Vanni Lughi
Research Activity

Overall project objective
This PhD research project is part of a wider project, which aims at developing a high
efficiency, nanostructured multiphase material for photovoltaic applications, characterized
by an electronic intermediate band (IB) located within the semiconductor bandgap. This
kind of material has shown to be feasible by enclosing semiconducor nanocrystals (NCs)
into a wider bandgap semiconductor (host material). This research project aims at realizing
this architecture through self-assembly and densification of heterophasic NCs (“coreshell”), synthesized by potentially low-cost, environmental friendly colloidal chemistry
approaches.

Activities performed during first year
The first year research activity focused initially on laboratory training and on reviewing
the available literature . Therefore, an optimal semiconductors system was chosen in terms
of costs, environmental remarks and optical properties (i.e. bandgap and relative position
of valence bands). Among metal chalcogenides, Cadmium Selenide or Telluride (CdSe or
CdTe) NCs embedded into a Zinc Selenide, Telluride or Sulfide (ZnSe, ZnTe or ZnS)
matrix were evaluated as composite systems. Hence, with respect to practical and
technological limitations, the CdSe and ZnSe NCs synthesis techniques were improved,
selecting favorable precursors/solvent/surfactants, to obtain NCs of the chosen size (and
therefore desired optical properties). Monophasic NCs, ranging from 2 to 6 nanometers
diameter, were successfully obtained. Afterwards, novel CdSe-ZnSe and CdSe-ZnS
heterophasic NCs (“core-shell”) synthesis techniques were developed, obtaining controlled
size , good monodispersed CdSe cores, with an overgrown ZnX (X=Se,S) shell layer with
tunable thickness. NCs solutions have been characterized through absorbance and
photoluminescence spectroscopy, during and after the synthesis; some films, selfassembled from NCs dispersions, have been optically characterized through
photoluminescence spectroscopy, and morphologically through Wide Angle X-ray
Spectroscopy (WAXS). Finally, TEM and HRTEM images were acquired, in order to
evaluate NCs size.

Objectives for the following year
o Improving NC deposition techniques on conductive substrates
o Scouting and improving densification procedures to obtain a completely inorganic
film, with required nanoscopic scale architecture and desired optical properties
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
56
Educational Activity

Classes followed, 1st year (substituted by personal study):
Books:
- S. Adachi, “Properties of group-IV, III-V and II-VI semiconductors”,
ISBN 0-470-09032- M. Winterer, “Nano-crystalline Ceramics. Synthesis and Structure”,
ISBN 3-540-43433-X
Reviews:
-
C.B. Murray et al., “Synthesis and characterization of monodisperse
nanocrystals and close packed nanocrystal assemblies”, Annu. Rev.
Mater.Sci. 30 (2000), 545-610
S. Kumar, G.D. Scholes, “Colloidal nanocrystal solar cells”,
Microchim. Acta 160 (2008), 315-325
- A.J. Nozik, “Quantum dot solar cells”, Physica E 14 (2000), 115-120
- J. Park et al., “Synthesis of monodisperse spherical nanocrystals”,
Angew. Chem. Int. ed. 46 (2007), 4630-4660
-

Laboratory hours, 1st year
o About 1000 hours of work at DMRN3 labs.
o 20 hours of WAXS e SAXS spectroscopy at ELETTRA SAXS beamline 4

Conferences and seminars organized by Nanotechnology graduate school
M. Fermeglia, “Utilizzo del VPN e presentazione del portale della scuola di
Nanotecnologie”, Università degli Studi di Trieste, 20.03.2009
o “Incontro formativo per i dottorandi”, Università degli Studi di Trieste, 30.06.2008
o C. Blasetti, V. Sergo, M. Fermeglia, “How to write (and manage) a research
project”, Università degli Studi di Trieste, 16.10.2009
o “Activity presentation of the III year phd students and CENMAT presentation”,
Università degli Studi di Trieste, 26.11.2008

Conferences, seminars, advanced courses and other didactic activities
o M. Fermeglia, G. Fraziano, F. Russo, V. Prodi, “Energie rinnovabili: una sfida e
una opportunità per la ricerca e le imprese europee”, Università degli Studi di
Trieste, 28.05.2009
o V. Lughi (DMRN), “Approcci chimici alla fabbricazione di materiali fotovoltaici
nanostrutturati”, Università degli Studi di Trieste, 24.09.2009
o P. Posocco (DICAMP5), “Modellistica molecolare multiscala per la progettazione di
materiali nano strutturati”, Università degli Studi di Trieste, 24.09.2009
o A. Jarosik (Max Plank Institut6), “Percolation of oxide networks in composite
electrolytes”, DMRN, 12.10.2009
3
Dipartimento dei Materiali e delle Risorse Naturali, Università degli Studi di Trieste, via Valerio 6A, I-34127 Trieste, Italy
SAXS beamline, Sincrotrone Trieste, Strada Statale 14, km 163,5 AREA Science Park, I-34149 Basovizza, Trieste, Italy
5
Dip. di Ingegneria Chimica, dell‟Ambiente e delle Materie Prime, Univ. degli Studi di Trieste, Piazzale Europa 1, I-34127 Trieste, Italy
6
Max Plank Institut für Festkörperforschung, Heisenbergstraße 1, D-70569 Stuttgart, Germany
4
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
57
Scheda del dottorando Elisa D’Este, [email protected], [email protected]
Titolo della tesi: : BIO-SAMPLE ENVIRONMENT MANIPULATION USING
ADVANCED MICROSCOPY TECHNIQUES
Supervisore: prof. Alberto Morgante, dott. Dan Cojoc
Tutori (eventuali): dott. Enrico Tongiorgi
Research activity
Neurotrophins are a family of proteins that induce the survival, development and function of
neurons. Brain-derived Neurotrophic factor (BDNF) is a member of this family and is able
to induce different cellular responses according to the site of stimulation of neurons
(McAllister, Neuron 1995). During this year I developed a system that allows a high spatial
resolution delivery of this protein. This system is based on silica beads (1.5um) manipulated
with optical tweezers and places on the dendrites of hippocampal neurons. I demonstrated
that BDNF is still biologically active when covalently attached to the beads analyzing C-Fos
translocation into the nucleus (Finkbeiner, Neuron 1997) and calcium levels (Berninger,
NeuroReport 1993). We saw that BDNF-coated beads induce C-Fos translocation while
control beads coated with Bovine Serum Albumin (BSA) do not. Moreover, using Optical
Tweezers, I stimulated neurons with a BSA-bead, I recorded calcium signal for 10 minutes
and then I placed the second bead on another dendrite. This second bead could be a second
BSA-bead (control experiments) or a BDNF-bead. Signal was then recorded for 50min.
Stimulation of pyramidal neurons with BDNF-beads induced an increase in calcium levels
both in the somas and in the stimulated dendrites and this signal is much higher then the one
obtained with BSA beads.
In this year I developed a new tool for local stimulation of neurons that allows a researcher
to have the experiment and the control on the same cell (really usefull for
electrophysiologists). This technique can be of interest also in the field of molecular
neurobiology in order to analyze the effect of molecules on different part of a same cell.

Goals for the next year
o Setting of sperimental conditions to visualize mRNA and Endoplasmic Reticulum
movements in vivo using fluorescence microscopy
o Development of new vectors that prevent mechanical stimulation of cells
o Building of a an optical tweezers setup coupled to a UV microdissection setup
o Building of a multi-trapping setup
 Pubblicationms
“The evolutionary conserved gene C16orf35 encodes a nucleo-cytoplasmic protein that
interacts with p73.” Lunardi A, Chiacchiera F, D'Este E, Carotti M, Dal Ferro M, Di
Minin G, Del Sal G, Collavin L. Biochem Biophys Res Commun. 2009 Oct
16;388(2):428-33.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
58


Pubblications/abstracts in conferences)
o ORAL:“Local BDNF delivery by means of laser tweezers to stimulate hippocampal
neurons signalling” Elisa D‟Este, Gabriele Baj, Paolo Beuzer, Enrico Ferrari,
Federica Tavano, Enrico Tongiorgi, Dan Cojoc; ICTP-Introduction to
Optofluidics
o ORAL: “High spatial resolution BDNF delivery by means of laser tweezers to
stimulate hippocampal neurons signalling”; Elisa D‟Este, Gabriele Baj, Paolo
Beuzer, Enrico Ferrari, Federica Tavano, Enrico Tongiorgi, Dan Cojoc; EBSA
2009
o ORAL: “High Resolution Drug Delivery by Optical Tweezers Microscopy” Elisa
D‟Este, Gabriele Baj, Paolo Beuzer, Enrico Ferrari, Federica Tavano, Enrico
Tongiorgi, Dan Cojoc; Super and High Resolution Imaging 2009
o POSTER: “Optical Tweezers as means to stimulate hippocampal neurons with
BDNF coated beads”; Elisa D‟Este, Gabriele Baj, Paolo Beuzer, Enrico Ferrari,
Federica Tavano, Enrico Tongiorgi, Dan Cojoc; Nanobioviews-2nd international
symposium on Optical Tweezers in life sciences; Award best poster Optical
Tweezers
Educational activity
Ore di lezione seguite I anno (corso, data/periodo, docente, tipo di corso di laurea)
o Neuroscienze; aprile-maggio 2009; prof. Leanza; CDLM in Biotecnologie mediche
20 ore
o Advanced Imaging and Spectromicroscopy methods for chemical and structural
characterization of micro- and nano-materials; 11-15 maggio 2009; Dott.sa Maya
Kiskinova; 15 ore
o Neuronal Calcium signaling; novembre 2009; dott.sa Giulietta Pinato; CdLM in
Neuroscienze; 4 ore
o Ottica e Microscopia avanzata; gennaio-luglio; Dan Cojoc; 25 ore
o Lettura libri: 5 ore
“Mechanics of Motor Proteins and the Cytoskeleton”; Howard
o Lettura review: 15 ore
A practical guide to Optical trapping; Shaevitz;
Angels and demons: neurotrophic factors and epilepsy; Simonato et al.;
Trends in Pharmacological Sciences 2006
Activity-dependent expression of BDNF in dendrites: Facts and open
questions; Tongiorgi; Neuroscience research 2008
Neurotrophin-regulated signaling pathways; Reichardt; Phil. Trans. R. Soc
2006
Signaling by death receptors in the nervous system; Haase et al.; Curr. Opon
in Neurobiology 2008
Role of the BDNF at glutamatergic synapses; Carvalho et al.; Brit. J.
Pharmacology 2008
Molecular mechanisms of dendritic spine development and maintenance;
Sata et al.; Acta Neurobiol Exp 2008
Development and regulation of dendritic spine synapses; Calabrese;
Physiology 2006
RNA trafficking and local protein synthesis in dendrites: an overview;
Martin and Zukin; J. of Neuroscience 2006
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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From Synapse to Nucleus:Calcium-dependent Gene Transcription in the
control of Synapse Development and Funcion; Greer and Greenberg;
Neuron 2008
Calcium and synaptic plasticity; Cavazzini et al.; Cell Calcium 2005
Calcium Signaling in dendrites and spines: pratical and functional
considerations; Higley and Sabatini; Neuron 2008
Multiple Roles of the p75 Neurotrophin Receptor in Nervous System; Chen
et al.; J. of Int. Med. Research 2009
 Ore di laboratorio svolte I anno (corso, data/periodo, docente, tipo di corso di laurea)
La gran parte del lavoro (> 60 ore) è stata svolta presso il laboratorio di neurobiologia del
dott. Enrico Tongiorgi (dipartimento di biologia) e nel laboratorio di ottica presso il CNRINFM-LN TASC sotto la supervisione del dott. Dan Cojoc.

Congressi, seminari ed incontri organizzati dalla scuola
o Congresso della scuola in nanotecnologie; gennaio 2009; 9 ore
o Seminario VPN; 20 marzo 2009; 2 ore
o Seminario Modeling reactions at the nanoparticle scale; Prof. Phillip R.
Westmoreland Dept; 8 maggio 2009; 2 ore
o Seminario presentazione CENMAT e dottorandi III anno; 26 novembre 2009; 4 ore

Corsi avanzati seguiti – seminari – altre attività didattiche passive
o Congresso dei biotecnologi italiani; 6-7 marzo 2009; 15 ore
o Corso sicurezza Laser; Elettra 25 marzo 2009; 4 ore
o Workshop “AFM per campioni biologici”; 5 maggio 2009; Bologna, 6 ore,
o Workshop “Orion Plus – The Ultimate Resolution”; 28 maggio 2009; Milano; 5 ore
o School Introduction to optofluidics; ICTP 1-5giugno 2009; Trieste; 40 ore circa
o Corso teorico/pratico intensive di microscopia a scansione elettronica; Trieste; 18
giugno 2009; 6 ore
o EBSA 2009 European Biophysic congress; 11-15 luglio 2009; Genova; 30 ore
o Joint Workshop between the SFB762, the Nano-IMPRS and the Scuola di
Dottorato di Ricerca in Nanotecnologie; settembre 2009; Wittenberg; 15 ore circa
o Super and High Resolution Imaging 2009; 30 settembre-2ottobre 2009; Lipica
(Slovenia); 20 ore
o NanoBioViews: 2nd International symposium on Optical Tweezers in life sciences;
14-15 ottobre 2009; Berlino (Germania); 16 ore
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Graduate Student: CATERINA ELIA [email protected]
Title of the thesis: Molecular Interaction on Nanoscale in Cancer Therapy
Supervisor: Prof. Tullio Giraldi
Tutors (if any): Dr. Giuseppe Toffoli
Activity
This project aims to analyze the molecular interactions on nanoscale for combination therapy in
cancer patient to optimize therapy. Part of the first period was spent in research and systematic
evaluation of all relevant literature. After it was conducted a bibliographic research on PubMed, and
clinical protocols have been defined. It was also planned procedures for the collection and
processing of biological samples, the construction of databases and the development of analytical
methods.
It was examined two protocols: "First line treatment with exemestane in patients with metastatic
breast cancer" and "Phase I study docetaxel, oxaliplatin, capecitabine (DOC) in untreated patients
affected by gastric cancer". The latter is a phase I study to define the maximum tolerated dose
(MTD) in patients with gastric cancer. It has been evaluate the procedures for analysis and
validation for the exemestane and docetaxel in biological matrices using HPLC-MS/MS (high
performance liquid chromatography coupled with tandem mass spectrometry). The analytical
methods were validated for specificity, reproducibility, accuracy, linearity, precision, limit of
detection and limit of quantification. The time of analysis for the exemestane and docetaxel was 5.0
and 3.0 minutes respectively. The limit of quantitation for exemestane was 0.19 ng/ml, while for
docetaxel is equal to 0.15 ng/mL. The methods have a range of linearity of 0.19-51.2 ng/mL for the
exemestane, and 0.15-1500 ng/mL for docetaxel with r2≥0998. So far, two complete
pharmacokinetic for the exemestane and eleven for docetaxel have been investigated. Analysis of
pharmacokinetic data showed a slight trend in the docetaxel AUC, however, at present, still limited
number of patients investigated allowed us to observe significant differences. Clinical studies are
still in progress. Investigation will be completed by 2010.
Objectives for the following year (if applicable)
x Continue with the recruitment of patients to determine and assess the MTD of Docetaxel in
patients with gastric cancer and to define the toxicity profile.
x Continue with the enrolment of patients with breast cancer to determine plasma levels of
exemestane on the response and toxicity to treatment
x Development and validation of additional analytical methods for the analysis of
pharmacokinetic interaction in combination therapy used in specific treatment protocols in place
at the CRO of Aviano.
Publications on scientific journals (printed or in press)
Corona G, Elia C, Casetta B, Diana C, Rosalen S, Bari M, Toffoli G. A liquid chromatographytandem mass spectrometry method for the simultaneous determination of exemestane and its
metabolite 17-dihydroexemestane in human plasma. J Mass Spectrom. 2009 Jun.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Corona G, De Lorenzo E, Elia C, Simula MP, Avellini C, Baccarani U, Lupo F, Tiribelli C,
Colombatti A, Toffoli G. Differential protomi analysis of hepatocellular carcinoma. Internation
Journal of Oncology 2009 (accepted for publication)
Publications/abstracts in conferences/congresses (national or international)
Corona G, Elia C, Casetta B and Toffoli G. Liquid Chromatography/Tandem Mass
Spectrometry Method To Rapid Asses the Irinotecan Metabolic Phenotype. Mass Spec Europe
5-6 November 2009, Barcelona, Spain
Educational Activity
Periods abroad (date and place)
Classes followed (date, course, professor, type of course)
200 hours of study and deepening of the topics covered by books and review-type paper and/or
electronic form (on 031209)
1350 hours at "Experimental and Clinical Pharmacology" CRO Aviano headed by dr. Giuseppe
Toffoli (on 031209)
Conferences, seminars, advanced courses and other didactic activities
“Congresso annuale delle presentazioni dell’attività scientifica dei dottorandi in corso” at
University of Trieste on January 13, 14 and 15, 2009 from 9.00 to 18.30
“Seminario sull'uso della VPN e del Portale” at University of Trieste on March 20, 2009 at 15.00
“Modeling reactions at the nanoparticle scale” Prof. Phillip R. Westmoreland Dept of Chemical
Engineering, University of Massachusetts Amherst, at University of Trieste on May 8, 2009 at
15.00
“Chimica fisica delle interfasi in sistemi nanostrutturati” Dr. Italo Colombo, at University of
Trieste on May 15, 2009 at 11.00 am
“Incontri informativi per i dottorandi: orientamento all’utilizzo delle risorse e dei servizi
documentali forniti dal Sistema Bibliotecario di Ateneo; panoramicadelle nuove modalità di
comunicazione scientifica: l’archivio istituzionale di Ateneo OpenstarTs; l’autoarchiviazione
delle tesi di dottorato e cenni sull’accesso aperto alla letteratura di ricerca; procedure di
conferimento al sito login miur e all’anagrafe di Ateneo (Saperi) at University of Trieste on
June 30, 2009 from 9.30 am to 12.30 pm
“How to write (and manage) a research project” at University of Trieste on October 16, 2009
from 14.00 to 19.00
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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“Tumori in HIV in era HAART - IX riunione nazionale” at CRO Aviano on January 16, 2009
from 8.30 to 17.30
“HIGHLIGHTS IN ONCOLOGY. Recenti acquisizioni in oncologia. CRO Aviano” at
Convention Hall - Pordenone Fiere on February 11, 2009 from 8.00 to 16.45
“Beyond UHPLC Seminar Series. The Universal Solution for fast LC” at the Hotel Minerva Pordenone on March 05, 2009 from 9.00 am to 13.00 pm
“CRO INCONTRA IMPRESA. Tech Transfer e ricerca oncologica” at CRO Aviano on April
24, 2009 from 9.00 to 13.30
“Seminari in Oncologia al CRO di Aviano” at CRO Aviano on May 28 and June 4-11-18, 2009
from 16.00 to 19.00
“La donazione di sangue, cellule staminali, cordone ombelicale: un’opportunità e una risorsa”
at CRO Aviano on October 03, 2009 from 08.20 to 15.30
“Utilizzo delle cellule staminali ematopoietiche in pediatria: indicazioni attuali e prospettive
future” at MIB School of Management - Trieste on June 30, 2009 from 09.00 to 16.00
“English language course at the Shenker Institute of Pordenone”: January to July 2009, 20
hours of lessons with final certificate
“BLSD - Corso teorico pratico di Basic Life Sup port and Defibrillation” at CRO Aviano on
April 15, 2009 from 8.45 am to 18.00 pm
“REFERENCE MANAGER: uno strumento straordinario per ottimizzare la gestione delle
bibliografie” at CRO Aviano on May 19 and 26, 2009 from 14.00 to 17.30
“Course Hamilton Microlab Star (HMS)” Training at Experimental and Clinical Pharmacology
CRO Aviano on September 29-30, 2009
Support educational activity and teaching
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Graduate Student: DANIEL ERA
Title of the thesis: Nanotechnological application in pharmacogenetic research
Supervisor: Prof. T. Giraldi
Tutors: Dr G. Toffoli
Research Activity
The primary objective of the research project is to evaluate the possible application of innovative
nanotechnology and methodologies in clinical practice of community medicine. The project is
aimed to evaluate the feasibility of the translation of pharmacogenetics approaches from clinical
trials, necessarily conducted on fewer samples, to current clinical practice of community medicine.
Detailed analysis of the literature indicates that pharmacogenetics of warfarin is relevant for a more
appropriate use of this drug. Warfarin is a widely used oral anticoagulant drug but its use has
problems due to individual variability in response, with the need to adapt dose for each patient.
Such individual variability is due to the presence of Single Nucleotide Polymorphisms (SNPs) in
genes encoding the enzymes involved in its metabolic inactivation (CYP2C9), and its biological
target, vitamin K epoxide reductase (VKOR), with resistance (persistence of risk of thromboembolic phenomena) or limited response to treatment (bleeding).
The objective of the research project even involve the validation of algorithms of prediction of
warfarin dose based on pharmacogenetic variables (polymorphisms of CYP2C9 and VKORC1).
The pharmacoeconomic aspects will be also considers in this study. From this evaluation may
derive any indication of the possibility of develop advanced nanotechnology devices, also suitable
for use in clinical practice and community medicine.
The first year of PhD was dedicated to the development of the project, through extensive analysis of
the literature, writing the research protocol in collaboration with the clinicians, and the
identification of different methodological and technological approaches. The identified molecular
genetic techniques are Polymerase Chain Reaction (PCR), followed by analysis of polymorphisms
using technologies such as Pyrosequencing or TaqMan method. The project was then submitted and
approved by Ethical Committee of the Azienda per i Servizi Sanitari di Trieste n.1 (ASS1), because
the study covers patients recruited by the Centro Cardiovascolare and Distretto 1 of ASS1. These
structures have currently begun the enrolment of the patients and the organization of the clinical
records in order to create a database.
In the final part of the first year it is also started the collection of biological samples (blood or oral
mucosal cells), obtained after written informed consent from each patients recruited, for the analysis
of polymorphisms.
Objectives for the following year
Over the next two years, the doctoral project will develop along the following main points:
 Patients enrollment and implementation of the database with the data of their medical
records;
 collection of biological samples;
 DNA extracting and genptyping;
 statistical analysis of the data;
 pharmacoeconomic analysis of the results;
 writing a conclusion and final report.
Publications/abstracts in conferences/congresses (national or international)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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o Role of genetic polymorphism 5-HTTLPR of serotonin transporter in psychological response to
diagnosis of breast cancer. XI national congress of Italian Society of Psycho-Oncology (SIPO).
October, 1-3, 2009, Senigallia (An) (POSTER);
o Genetics interaction between citalopram antidepressant activity and MAO-A VNTR
polymorphism in paliative care of cancer. 34° national congress of Italian Society of Pharmacology
(SIF). October, 14-17, 2009, Rimini (POSTER).
Educational Activity
Classes followed (date, course, professor, type of course)
o In agreement with the supervisor and as provided by the school, the classes have been replaced by
the reading of numerous reviews and original papers on pharmacogenetics. In addition, during the
first year was made an extensive study on the statistical analysis of data through books, manuals
and use of dedicated programs (SPSS, systat, R).
o Laboratory of Pharmacogenetics, Department of Life Sciences, section of Pharmacology. Dr. G.
Schillani.
Conferences, seminars, advanced courses and other didactic activities
o Congress of the “PhD school in Nanotechnology”. January 2009;
o Congress “Highlights in oncology. Recenti acquisizioni in oncologia”. February 11 2009, Aviano
(Pn);
o III Meeting-Workshop “Nuove Prospettive in Chimica Farmaceutica”. February 13-14 2009,
Castelvecchio Pascoli (Lu);
o Prof. Maurizio Fermeglia “Uso del VPN per l‟accesso remoto”. March 20 2009, Trieste;
o Dr A. Bardelli “Cancer mutations and targeted therapies”. May 6 2009, Trieste;
o Prof. PR Westmoreland, Dept. of Chemical Engineering, University of Massachusetts Amherst,
“Modeling reactions at the nanoparticle scale”. May 8 2009, Trieste;
o Dr I. Colombo “Chimica Fisica delle interfasi in sistemi nanostrutturati”. May 15 2009, Trieste;
o Incontri informativi per i dottorandi organizzato dalla Sezione Ricerca e Dottorati in
collaborazione con il Sistema Bibliotecario di Ateneo. June 29 2009, Trieste.
o 34° National Congress of the Italian Society of Pharmacology “Il valore del farmaco per la tutela
della salute”. October 14-17 2009, Rimini;
o Corso introduttivo alla farmacoepidemiologia, ASS1-ISS, November 19-21 2009, Trieste.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Graduate Student: Raffaella Fior
Title of the thesis: “Ionic channel behavior: development of a novel, non destructive method
for mechanical and electrical analysis of a single cell”
Supervisor: prof. O. Sbaizero
Tutors (if any): Research Activity
The main goal of this doctoral activity is the design and the realization of an experimental technique
able to perform electrical and mechanical analysis of single living cells. In particular our research
will be focused on ion channel activity correlated to mechanical properties. The background idea is
to integrate micro-fabrication techniques (micro electro mechanical devices, MEMS) and an atomic
force microscope (AFM).
During the first year, we focused our research on the design and optimization of commercial
MEMS. We used these devices to analyze single cells, in particular we evaluated the morphological
modifications comparing the ratio between area and perimeter with the number and the type of
stimuli (linear, cyclic, amplitude and velocity of stretch). We also used shape factor to estimate the
healthy degree of each stimulated cell. However, commercial MEMS are non-transparent and
therefore every analysis must be performed with reflective microscopy. To overcome this problem,
I worked (and I am still working) in the nearby CNR-TASC laboratories and a transparent MEMS is
under realization. We designed the masks that we will use during fabrication, we modelled with
finite elements analysis each constitutive element, finally we chose the production techniques
(deposition techniques and etching) and selected the best materials. Thin films, using Plasma
Enhanced Chemical Vapour Deposition (PECVD), have been already deposited. Each composition
has been tested focusing on transparency, refractive index, impurities, deposition rate and etching
rate.
Objectives for the following year (if applicable)
Transparent MEMS
1. realization of a prototype
2. optical and mechanical characterization
Commercial MEMS PolyMUMPs
1. surface treatment to prevent cell adhesion in the measurement area
2. local functionalization of the platforms
3. single cell stress-strain evaluation
AFM analysis on fixed and living cells
1. cells over a conductive surface
Participation to conferences (as speaker)
“Mechanical and Electrical Analysis of a Single Cell” - HIM 2009 (Highlights in Microtechnology)
– Neuchatel, Switzerland 2-5 July 2009
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Educational Activity
Conferences, seminars, advanced courses and other didactic activities
Courses
1. 11-15 May 2009 “Advanced Imaging and Spectromicroscopy for Chemical and
Structural Characterization of Micro and Nano Materials” Maya Kiskinova Head of
Microscopy projects Synchrotron Trieste 15hours
School
1. 23rd August – 12th September 2009 “ESONN'2009 European School On Nanosciences
& Nanotechnologies” Grenoble, France
Poster presentations
1. “Development of tools for biologic applications” R. Fior, S. Maggiolino, B. Codan, E.
Merola, S. Rizzi and O. Sbaizero - R&D - University of Trieste 22nd May 2009
2. "Mechanical Analysis of Fibroblast Cell Line Using MEMS Technology” R. Fior, S.
Maggiolino and O. Sbaizero - ECI conference “Nanotechnology for the Study of
Cellular and Molecular Interactions” - Barga (LU) 14-18 June 2009
Poster award: best poster honourable mention
3. “Micro Electro Mechanical Systems for single cell analysis: design, production and
characterization” R. Fior, S. Maggiolino and O. Sbaizero - Euromat 2009 European
Congress and Exhibition on Advanced Materials and Processes - Glasgow, UK 7-10
September 2009
4. “Realization of a new MEMS device to mechanically stimulate a single cell: evaluation
of the morphological modification.” R. Fior, S. Maggiolino and O. Sbaizero ESONN'2009 European School On Nanosciences & Nanotechnologies - Grenoble,
France 23 August – 12 September 2009
Seminars
1. “Percolation of oxide networks in composite electrolytes” A. Jarosik - International
Max Planck Research School for Advanced Materials Stuttgart Germania @ DMRN
Università di Trieste 12th October 2009
2. "Structure Effect on Interfaces" by Francesco Stellacci - Department of Material Science
and Engineering, Massachusetts Institute of Technology @ELETTRA building
Basovizza TS 12th October 2009
3. “Focused on the Fibroblast: friend or foe? Bystander or therapeutic target” C. Long MD
- University of Colorado Health Sciences Center, Chief Cardiology Section Denver
Health Medical Center @polo cardiologico di Cattinara Trieste 4th December 2009
Support educational activity and teaching
-3 hours metallurgy seminar: starting materials for steel production, blast furnace, cast irons, ferrous
and non ferrous materials, metals designation, stainless steel.
-2 hours biomaterials seminar: cellular culture techniques
-100 hours biomaterials laboratory following 5 working groups:
1. bio compatibility analysis of 4 materials (UNI EN ISO 10993-5);
2. evaluation of cellular migration over a polymeric stressed material;
3. set-up of a cellular manipulation system: pico-spritzer;
4. preparation of polyacrylamide gel with different composition and different adhesion
proteins (polilysin, fibronectin, collagen IV) to evaluate cellular adhesion;
5. glass photolithography of a dental biocompatible polymer.
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Graduate Student: Valeria Lanzilotto
Title of the thesis: Self-assembling and charge transfer properties of thin organic films
Supervisor: Prof. Alberto Morgante
Tutors (if any):
Research Activity
During the first year of my PhD I had the opportunity to address different research topics mainly
focused on the study of both geometrical assembling and electronic properties of organic-inorganic
interfaces. In parallel I have investigated more traditional systems such as organic thin film on
metal or semiconductor substrates, and I began the study of systems such as the most innovative
devices built from individual molecules.
This allowed the use of different experimental techniques: spectroscopy (XPS, NEXAFS, ResPES),
diffraction (HASPES, PED, LEED) and microscopy (STM) for the traditional systems, while
conductivity measurements (STM break-junction method) for the study of the electronic properties
of single molecule devices.
So far the most significant results were achieved in the analysis of the ordering properties of C60
and pentacene thin films on TiO2 (in its semiconductor phase). Information concerning the
electronic properties and possible charge transfer are being finalized.
As part of research conducted in the field of single molecule electronics, numerous experiments
have been conducted in order to deepen the study of junctions Au/benzenediamine/Au in different
conditions (UHV and low temperature) than those reported in the literature (in air and room
temperature).
Objectives for the following year (if applicable)
The plain is to investigate on the missing information on the electronic properties of the
aforementioned systems (C60 and pentacene/TiO2); to this end a period of measurements
(beamtime) on the ALOISA/HASPES beamline has already been approved and allocated.
Futhermore the next step on the study of the single molecules conduction properties is to move
forward with the support of spectroscopic informations that can be obtained from the same thin
organic films on gold (in this case too we take advantage of the aforementioned beamtime).
Publications on scientific journals (printed or in press)
Publications/abstracts in conferences/congresses (national or international)
“Charge transfer properties of thin organic films : an outlook on single molecule devices”, 2nd
PhD Workshop of the SFB 762 in conjunction with the International Max Planck Resarch School
for Science and Technology of Nanostructures, Wittenberg, Germany, 10-11/09/2009
“One of the main goals of nanotechnology is the miniaturization of electronic devices through the
exploitation of unique properties of matter at the nanoscale. At present, many efforts have been
made to develop electronic devices in which a molecule between two electrodes plays the role of an
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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active conducting element. Although reliable and reproducible experiments have been reported, a
comprehensive conceptual understanding of electron transport in molecular system is lacking.
In this work, the main results obtained by means of a home-made STM break junction set up will be
presented. In the STM break junction technique an Au scanning microscope tip is repeatedly
crashed in and moved out of an Au surface in the presence of the molecule. As the Au point contact
is streched and finally broken, a molecule can be trapped between the tip and the substrate. It is
then possible to apply a bias to the electrodes and measure the current passing through the
molecule.
The electron tunnelling through the molecular junction is affected by the coupling between the
discrete energy levels in the molecule and the continuum states of the electrodes. To understand this
coupling, the single molecule conductance measurements will be discussed with the support of the
spectroscopic investigations of the same thin organic films on gold. In particular, by means of
Resonant Photoemission Spectroscopy it has been possible to disentangle molecular orbitals and
determine the ones involved in the charge transfer at the surface.”
Participation to conferences (as speaker)
“Charge transfer properties of thin organic films : an outlook on single molecule devices”, 2nd
PhD Workshop of the SFB 762 in conjunction with the International Max Planck Resarch School
for Science and Technology of Nanostructures, Wittenberg, Germany, 10-11/09/2009
Educational Activity
Periods abroad (date and place)
25/10/09-21/12/09 Instituto de Ciencia de Materiales (Universidad Autónoma de Madrid)
Classes followed (date, course, professor, type of course)
12/01/09-06/03/09 (48 ore), Spettroscopia atomica e molecolare, Prof. Alfonso Baldereschi, Corso
di laurea specialistica in Fisica (Curriculum: Fisica della Materia)
02/03/09-29/05/09 (48 ore), Elementi di ottica quantistica, Prof. Fulvio Parmigiani, Corso di laurea
in Fisica
11-15/05/09 (12 ore), Advanced Imaging and Spectromicroscopy methods for chemical and
structural characterization of micro- and nano-materials, Maya Kiskinova, Didattica XXIV Scuola
di Dottorato in Fisica
Conferences, seminars, advanced courses and other didactic activities
09/01/09 "First-principles studies of single-molecule junction conductance: Links, length, and
switching" Jeffrey B. Neaton, Molecular Foundry, LBNL, Berkeley, USA
13-15/01/09 Congresso Scuola nanotecnologie
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13/03/09 PhD briefing @ TASC - "Templated Growth and Properties of Iron Nanowires:
Anisotropic Electron States of the quasi 1D Copper Substrate, and morphological and magnetic
properties of the iron adsorbate" Carlos Eduardo Viol Borbosa - "Organic molecules at metal
surfaces: the role of functional groups" Martina dell‟Angela
20/03/09 Seminario VPN e Portale
08/04/09 Scuola di nanotecnologie: esame finale aprile 2009
30/06/09 Incontri Informativi Dottorandi
17/09/09 PhD briefing @ TASC - "Ultra fast charge transport in nanotubular alpha-MnO2 self
assembly studied by X-ray resonant photoemission", Gregor Kladink
12/10/09 "On the effect of Nanostructure on Interfacial Energy", Francesco Stellaci, Department of
Material Science and Engineering, Massachusetts Institute of Technology
16/10/09 PhD briefing @ TASC - "Design, realization and characterization of a novel
device: a transparent MEMS for mechanical single cell analysis", Raffaella Fior
01/12/09 "Surface Chirality Manifestation through the Surface Stress", Maria Blanco Rey,
Department of Chemistry, University of Cambridge
Support educational activity and teaching
22-27/04/09 Partecipazione all‟esperimento "Chemical state and structural characterisation of
adsorbed dipeptides" at ELETTRA Synchrotron lab in Trieste, Italy, “ALOISA/HASPES” beamline
16/10/09 PhD briefing @ TASC - "Thin organic films and their charge transfer properties: an
outlook on single molecule devices", Valeria Lanzilotto
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Graduate Student: IMMACOLATA LUISI
Title of the thesis: Identification of novel protein scaffolds for binding of small molecules
Supervisor: Prof. Roberto Marzari (University of Trieste)
Tutor: Prof. Daniele Sblattero (University of Eastern Piedmont)
Research Activity
In the literature, there are well known protein structures, named scaffold, used and modified for the
construction of variants able to recognize and bind small molecules. These scaffolds differ in terms
of structures stability, tolerance to multiple substitutions, ease of expression and subsequent
applications.
Protein scaffolds are divided according to the architecture of their bones into three groups: scaffolds
formed by -helix, scaffolds formed mainly by -sheets and small scaffolds with few secondary
structures or irregular architecture of -helices and -sheets. Among the alpha-helices scaffolds
there are the coiled-coil domains: these are composed by two right-handed alpha helices which
dimerize. A research group has synthesized ex-novo a scaffold structure named E/K coiled coil.
In my PhD project this E / K coiled-coil scaffold has been considered to isolate new molecular
interactors on the basis of some characteristics that have been already described. The E / K coiledcoil scaffold consists of two distinct peptides, formed by a single heptad repeated five times. The
sequence of the heptad for the E peptide is E-V-S-A-L-E-K while for the K peptide is K-V-S-A-LK-E (positions g-a-b-c-d-e-f). We decided to make the some changes to the basic structure of the
scaffold E/K in order to create a pocket that can bind various types of small molecules. Mutations
were made to both peptides E and K, in central heptads and leaving the other four heptads as wild
type. The strategy adopted was a random mutagenesis in which all amino acids were mutated in the
all positions of heptads considered. This randomization was obtained using a random design of
primers and subsequent cycles of PCR. A library of 2 * 108 single chain fragments E random / K
random was created. The quality of the library obtained was verified by PCR while the diversity
was validated by sequencing the clones. The sequence shown a good variability of each central
random heptad in all clones analyzed. The library has been selected with phage-display technique
on a range of analytes of our interest for the identification of novel protein scaffolds that are able to
bind these small molecules.
Objectives for the following year (if applicable)
For the future we will carry out a biochemical characterization of the clones selected against the
analytes chosen. In particular we will study the affinity, the stability, the folding, the constants of
association / dissociation, the chemical and physical characteristics (such as circular dichroism
spectra) and the three-dimensional structure of interacting partners for future applications in the
field biosensor as a new molecular scaffold.
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Educational Activity
Classes followed (date, course, professor, type of course)
11-15 May 2009: Course: “Advanced Imaging and Spectromicroscopy methods for
chimical and structural caracterization of micro and nanomaterials” Prof. Maya
Kiskinova, Elettra Trieste, course for phd student by nanotechnology school (15 hours)
18 June - 6 Julye2009: Course: “Molecular self-assembling and nanostructures” Prof. L.
Pasquato, A. Morgante e L. Casalis, course for phd student by nanotechnology school
(16 hours)
5-30 October 2009: Course: “Cellular Neurobiology” Prof. Enrico Tongiorgi, University
of Trieste, course of master degree in Neuroscience (24 hours)
5-30 October 2009: Course: “Techniques in cellular neurobiology” Dott. Gabriele Baj,
University of Trieste, course of master degree in Neuroscience (24 hours)
27 October 2009: Exercise Laboratory at the Department of Chemical Sciences for
measurements of CD spectrum (10 hours)
Conferences, seminars, advanced courses and other didactic activities
13-15 January 2009: Presentation of phd students of nanotechnology school
20 March 2009: “Use of the basic tools for activity of the school VPN (Virtual Private
Network )” Prof. M. Fermeglia
8 April 2009: Final exams of phd students of nanotechnology school
8 May 2009: “Modeling reactionsat the nanoparticle scale” Prof. Philip R. Westmoreland
15 May 2009: “Veneto-nanotech project” Dott.ssa Talarico; “Chemical physics of
interphases in nanostructure systems” Prof. Italo Colombo
16 October 2009: “Seminar on writing and managing of research project” Prof. M.
Fermeglia, Prof. V. Sergo e Dott.ssa Cecilia Blasetti
26 November 2009: “Activity presentation of the III year phd students and CENMAT
presentation”
Support educational activity and teaching
Tutors activities in the laboratory of cell biology for a bachelor's degree student in the field of
molecular biology for a total period of three months which includes the learning of key laboratory
techniques such as preparing solutions and culture media for bacterial growth, preparation of
glycerolates, preparation of chemical competent cells, bacterial transformation, plasmid DNA
extraction, PCR (Polymerase Chain Reaction), agarose gel electrophoresis, preparation of
acrylamide gels and Western blotting. Support in the writing of the three years thesis.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Graduate Student: Monica Marini
Title of the thesis: Implementation of simple logics in bioinorganic hybrid nanosensors
Supervisor: Giuseppe Firrao
Tutors (if any):

Research Activity
In this year I carried out the preliminary work for the development of the biosensing layer of a
novel hybrid nanosensor comprising a nanopore and complex nucleic acid structure. For this
purpose two methods were applied for the construction of an effective sensitive layer: the
Hybridization Chain Reaction to obtain DNA long polymers labelled with fluorescent (FITC) or
Electrochemiluminescent (Ruthenium) moieties, and the new approach of two dimensional DNA
origami. The first approach resulted highly reproducible in vitro under controlled temperature, and
the visualization of the polymers was very simple (both in the case of modified and not modified
polymers) by gel electrophoresis, by epifluorescence microscopy or ECL detection. The
implementation of the DNA origami approach required an extensive preliminary design phase, that
was performed by dedicated software packages (SARSE v.1.38/DNA origami package v.1.10,
NanoEngineer-1 ™ v.1.1.1 and caDNAno v.0.2 .3). These programmes were evaluated for their
advantages and disadvantages, and I managed to obtain a reliable final model only with the use of
the software caDNAno. The designed structure consists of 218 oligonucleotides for each strand of
M13mp18 present in solution and I am currently proceeding with the in vitro validation of the
computer model. In the same time the mechanism of switching of the DNA origami was
investigated. This change of conformation is based on the hybridization events occurring between a
single stranded DNA (linked to the sensitive layer) and a target molecule of diagnostic interest. In
the case of the detection of molecules of biological interest such as mycotoxins, the protocol has
been finalized to the development of a DNA aptamer specific for Zearalenone, adjusting a previous
reported SELEX protocol. The didactic activities of this year included university courses at the
universities of Trieste and Udine, workshops organized by the Graduate School in nanotechnology
and others, advanced courses followed at ELETTRA and a summer school at the University of
Karlsruhe.
Objectives for the following year (if applicable)
1.
2.
3.
4.
In vitro validation of the expected DNA origami structure obtained from computer modeling
by caDNAno. Preliminary visualization of the origami by gel electrophoroesis and TEM;
In vitro development of the ZEA specific DNA aptamer after its chemical modification and
the development of affinity agarose-based columns with DADPA branches;
Study of the switching mechanism working only with the hybridization between two single
stranded DNA.
Preliminary study on the introduction of Boolean logic for the selective switching of the
biosensing layers.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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
Educational Activity
Classes followed (date, course, professor, type of course)
Course: ELETTRA Maj 2009 (14 hours). Maja Kiskinova: “Advanced Imaging and
spectromicroscopy methods for chemical and structural characterization of micro- and
nano-materials”.
Course: University of Trieste June 2009 (4 hours). Alberto Morgante, Loredana Casalis, Lucia
Pasquato: “Molecular self-assembling and nanostructures”.
Course: ELETTRA June – July 2009 (16 hours). Cristina Afric, Laura Felisari: “Advanced
microscopy: Scanning and Transmission Electron Microscopy, Scanning Probe
Microscopy”.
Course: University of Udine, First level degree in Mechanical Engineering, October 2009 January 2010 (60 hours). Di Giusto Sandro: “Fondamenti di informatica”.
Study of the Systematic Evolution of Ligands by EXponential enrichment and non-SELEX
techniques for aptamer development.
Study of the chemical modification of mycotoxins (Zearalenone) and its ligation to substrates as
paramagnetics beads and agarose-based resin in presence of DiAminoDiPropyl Amine.
Study of the DNA-based origami techniques for 3D and 2D structures. Application of the related
softwares as SARSE v.1.38/DNA origami package v.1.10, NanoEngineer-1™ v.1.1.1 e
caDNAno v.0.2.3
Conferences, seminars, advanced courses and other didactic activities
Activities organized by the School

PhD students activities presentation (20 hours): 13th - 15th January 2009.
Seminar: April 2009 (1 hour) University of Trieste “Nanochallenge and Polymerchallenge”.
th
Seminar: 15 May 2009 (1.5 hours) University of Trieste. Dr. Italo Colombo “Chimica fisica delle
interfasi in sistemi nanostrutturati”.
th
Seminar: 30 June 2009 (3 hours) University of Trieste. “Information meeting for doctoral
students”.
th
Seminar: 16 October 2009 (5.5 hours) University of Trieste. “Writing (and managing) a research
project”.
Other seminars
th
Seminar: 4 February 2009 (2 hours). Natural Science Museum of Udine. Ian Tatterstall: “Charles
Darwin and human evolution”
th
Seminar: 11 June 2009 (4 hours). Area Science Park. KeyToNature Technology Transfer Day:
“L'identificazione rapida di organismi: dalla ricerca alle applicazioni industriali”.
th
Seminar : 11 June 2009 (2 hours). University of Udine. Prof. Robert S. Marks: “Biosensor
systems in detecting patient exposure to pathogens and monitoring water toxicity: how
combining biology, physics and chemistry helps gain in sensitivity and time.”
th
th
Summer School: 7 - 11 September 2009 (26 hours). Universitat Karlsruhe (TH), DFG-Centrum
fur Functionelle Nanostrukturen. Bad Herenhalb, Germany: “CFN Summer School on NanoBiology”.
Support educational activity and teaching
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Dottorato: Elisa Migliorini
Titolo della tesi: The effect of nanotopography on cell differentiation: an alternative way
to guide embryonic stem cells into neurons.
Supervisore: Marco Lazzarino
Tutori (eventuali): Elisabetta Ruaro
Research Activity
Description of the research activity and the main results obtained:
The aim of my Phd project is to develop nanopatterned substrates which can stimulate, control and
direct the Embryonic Stem (ES) Cells differentiation into neurons.
My activity is divided in 3 technological different fields : 1- micro and nano fabrication to obtain
nanopatterned substrates, 2-Embryonic Stem Cells colture and differentiation into neurons, 3development of imaging techniques and methodologies for cell analysis in physiological conditions
with nanometrical resolution .
I fabricate several PDMS substrates for cells colture using Soft Lithography. These substrates have
simple 2D geometry (lines, pillars, circle) , periodicity between 180 nm and 800 nm and depth
between 100-500 nm.
PDMS replica obtained were analyzed using Scanning Electron Microscopy (SEM) and Atomic
Force Microscopy (AFM) on air and in liquid.
I differentiate Embryonic Stem cells into neurons using a stromal cell-derived inducing activity
(SDIA) protocol. Neuronal precursors were plated on glass coverslips and on glass coverslips
coated with PDMS because a first important result was the significant increase of cells vitality
observed on PDMS substrates. Moreover preliminary results show that the yield of differentiation
increase on nanopillars 250 nm wide and 200-400 nm deep.
To observe the cell-substrate interaction with micrometrical resolution I developed an imaging
methodology using AFM (in liquid). Neurons after 24 hours of differentiation were fixed and then
imaged with AFM in liquid.
An important goal reached this year was to find the ideal condition to perform AFM live imaging of
neurons on flat glass. Neuronal growth cones movements were observed for a couple of hours and
their growth and retraction were imaged. That result might be a perfect tool to study cell-substrate
interaction at nanometrical level.
Objectives for the following year (if applicable)
1- Fabrication of different patterns, with different aspect ratio, varying their period, depth and
mechanical properties. Fabrication of disordered patterns with the same dimension of the
previous ones. The patterns which induce a cellular response will be transfer on a Silicon
Nitride substrate.
2- Increase the yield of differentiation and the reproducibility of ES-derived neuronal colture.
Verify with a bigger statistical analysis the effect of the nanopatterned substrate (PDMS
pillars with a period of 250 nm) on the neuronal differentiation using immunofluorescence
essay and fluorescence Microscope. Gene expression analysis of ES-derived neurons grown
on nanopatterns.
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3- AFM on a bigger number of neuronal growth cones on nanopatterns in liquid after fixation,
to analyze the effect of the pattern on the GC‟s morphology. AFM of live neurons pleated
on PDMS flat and patterned to observe the interaction between neurons and substrates. AFM
to study adhesion forces, in particular, relatively to a single focal point.
Pubblicazioni su riviste scientifiche (pubblicate oppure in stampa),
 Migliorini E., Ban J., Di Foggia V., Ruaro ME., Torre V., Pozzato A., Grenci G., Tormen
M, Lazzarino M. Effect of nanopatterned substrates on growth cones activity. Eur Biophys
.J. 2009 Oct, (suppl 1) S89
 Kondra S, Laishram J., J. Ban J.,Migliorini E., Di Foggia V., Lazzarino M., Torre V., Ruaro
M.E. Integration of Confocal and Atomic Force Microscopy. Journal of Neuroscience
methods. 2009 Feb 15;177(1):94-107. Epub 2008 Oct 18.
 Laishram J., Kondra S., Avossa D., Migliorini E., Lazzarino M., Torre V. A morphological
analysis of growth cones of DRG neurons combining Atomic Force and Confocal
Microscopy. Journal of Structural Biology. 2009 Sept.
Partecipazione a congressi (come relatore)
 “AFM live imaging of neuronal growth cones reveals a new nanometrical morphology”.
SLONANO 2009, 19-21 October Lubjana, Slovenia. Oral presentation
 “AFM live imaging to analyze neuronal differentiation on nanopatterns” First Interim
meeting Nanoscale project. 23 Sept. 2009, Paris France. Oral presentation.
 “AFM live imaging of ES-derived neurons and growth cones retraction at the nanoscale”.
European Biophysics Congress EBSA: 11-15 July 2009. Genoa – Italy. Poster presentation
Attività formativa
Esami sostenuti, ore di lezione e di laboratorio seguite (data, corso, docente, tipo di corso di
laurea)
Corsi
Data
Num. ore
Docente
Tipo do corso
Structural biology
02/09
24
Silvia Onesti
SISSA (biofisica)
Advanced
Imaging 04/09
Spectromicroscopy
15
Maya
Kiskinova
S.di
Dott.Nanotecnologie
Optofluoidic School
06/09
40
Dan Cojoc
ICTP summer school
SEM
04/09
4
Laura
Felisari
TASC lectures
Laboratorio
Data
Num. ore
Tutors
Attività svolta
CNR-TASC
Dal 01-09
>60
M. Lazzarino
G. Grenci
Nano-micro
fabbricazione
CBM
Dal 01-09
>60
M.Lazzarino
AFM
prof. Dal 01-09
>60
Elisabetta
Ruaro
Jelena Ban
Colture di cellule
staminali embrionali
SISSA (lab.
Vincent Torre)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Congressi, seminari, corsi avanzati e altre attività didattiche passive
Congressi
Data
Ore
Organizzatore
EBSA Genova 2009
11-15/07/09
35
Alberto Diaspro
meeting 23/09/2009
Project
8
Vincent Torre
write (and 16/10/2009
a
research
4
Maurizio Fermeglia
20
Dragan Mihailović, J. Stefan Institute
First interim
Nanoscale
(Parigi)
How to
manage)
project
Slonano (Lubiana)
19-21/10/09
Seminari
Data
Relatore
High-resolution scanning X-ray diffraction 22/01/2009
microscopy
Dr. Pierre Thibault
X-ray microscopy:dealing with real-life 22/01/2009
complexities
Dr. Chris Jacobsen
Neuronal plasticity
Prof. Francois Anserment &
Prof. Pierre Magistretti
08/04/2009
Modeling reactions at the nanoparticle 08/05/2009
scale
Prof. Phillip R. Westmoreland
Calcium control of gene regulation in an 15/06/2009
Dr. Giulietta Pinato
in-vitro model of neuronal
plasticity
How is Enzymatic Activity Determined by 20/07/2009
Protein Dynamics?
Dr. Kerstin Blank
Inhibition of scrapie prion replication by 22/07/2009
polyelectrolyte nanogold particles &
Coated Nanogold and the Brain
Dr. Fernanda Sousa
Dopaminet summer school (CBM)
Prof. Stefano Gustincich
5-6/10/09
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Graduate Student : Seid-Hossein Pakdast
Title of the thesis: Advanced MEMS Resonators for High Sensitivity Molecular Detection
Supervisor: Marco Lazzarino
Tutors (if any): Research Activity
The goal of this project is to fabricate a mechanical mass sensor with a very high sensitivity down
to a few molecules.
A new geometry consisting in three-coupled microcantilevers simulated by finite element analysis
method to find the different resonance frequency modes and to see any correspondence between the
experiment and the simulation.
In this way, we can have a good estimation of the resonance frequency before fabricating the
device. The most interesting mode is the anti-symmetric one where the middle cantilever is not
moving. In principle, the added mass breaks the symmetry of the three-coupled microcantilevers,
which triggers the onset of nonzero vibration amplitude of the middle cantilever. The results of the
simulations show clearly that placing an additional mass on one of the two outer microcantilevers
will trigger the middle one to oscillate. Further, simulations on displacement amplitude show that
there is always present of transversal vibration.
The same geometry has been fabricated in the cleanroom at TASC by photolithography technique,
reactive ion etching, and wet etching processes. Scanning electron microscopy has been used to
characterize and to optimize fabrication processes.
The resonance modes are generated in vacuum by a piezoelectric actuator which oscillate the entire
chip or by a frequency-modulated laser in a custom built setup which is focused on individual
devices. The measurements showed that laser actuation method results in narrower mode peaks
since local actuation does not excite spurious modes which are instead observed by piezoelectric
actuation. The setup also allows to rotate the photodetector in order to measure the oscillation of the
cantilevers both along transverse and longitudinal axes. The results of the experiment show that
there is angle dependence of the cantilevers in both actuation methods.
Objectives for the following year (if applicable)
-Fabricate a number of devices with different designs and dimensions
-Optimize the fabrication process
-Optimize the setup in order to measure the thermo-fluctuations of the cantilevers
-The laser spot needs to be in order of 3-5µm in diameter
-Simulation of the real external forces e.g. laser beam or piezoelectric in order to have an
idea about the amplitude oscillations.
-Test the sensitivity with on purpose added masses.
Other publications
07/28-09, Abstract for Max-Planck Institute MPI, Workshop in Wittenberg from 09/10-09 to
09/11-09
Participation to conferences (as speaker)
 Max-Planck Institute MPI, Workshop in Wittenberg from 09/10-09 to 09/11-09
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Educational Activity
Classes followed (date, course, professor, type of course)
Date
Course
Professors / Teachers
Type of course
Hours
03/23-09
Biological Physics
Michele Vendruscolo
Biophysics
18
05/11-09 to
05/15-09
Advanced Imaging and
Spectromicroscopy methods for
chemical and structural
characterization of micro- and
nano-materials
Maya Kiskinova Head
of Microscopy projects
Sincrotrone
Overview of different
spectromicroscopies
15
06/01-09 to
06/05-09
Introductions to Optofluidics
J. Niemela
microfluidics with
MEMS
40
06/23-09 to
07/15-09
Advanced Microscopy (Electron
Microscopy and Scanning Probe
Microscopy)
Cristina Africh and
Laura Felisari
Microscopes
16
06/18-09 to
07/06-09
Molecular self-assembling and
nanostructures
Alberto Morgante,
Lucia Pasquato and
Loredana Casalisv
Biological,
nanotechnology
16
10/05-09 to
11/13-09
Photoemission spectroscopies
and spectromicroscopies
Alessandro Baraldi,
Andrea Goldoni and
Andrea Locatelli
surface science
25
Silvio Modesti
Semiconductor
physics
72
Total hours
202
28/09-09 to Experimental physics Laboratory
18/12-09
Conferences, seminars, advanced courses and other didactic activities
o4 hours, How to write (and manage) a research project, prof. Maurizio Fermeglia, period
16/10/09
Seminars 2009
 01/22/09, X-ray microscopy: dealing with real-life complexities, Chris Jacobsen
 01/22/09, High-resolution scanning X-ray diffraction microscopy, Pierre Thibault
 02/17/09, Cavity Quantum Electrodynamics with Single Quantum Dots, Anntonio Badolato
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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 02/18/09, Ordered Oxides: polarized x-ray spectroscopy of digital manganite superlattices,
Jerald J. Kavich
 03/13/09, Templated Growth and Properties of Iron Nanowires: Anisotropic Electron States
of the quasi 1D Copper Substrate, and morphological and magnetic properties of the iron
adsorbate, Carlos Eduardo VIOL BARBOSA
 03/13/09, Organic molecules at metal surfaces: the role of functional groups in self-assembly
and charge transfer, Martina Dell'angela
 03/16/09, Electronic structure of transition metal and rare earth ions in complex materials by
resonant core-level spectroscopies, Emanuela Carleschi
 03/31/09, Engineering photonic structures for THz devices, Alessandro Tredicucci
 05/08/09, Targeted Polymeric Coacervates for cancer therapy, Subhra Mandal
 05/08/09, reactions at the nanoparticle scale Reactive Molecular Dynamics (RMD)
Modeling, Phillip R. Westmoreland
 10/22/09, SuMBD growth of oligothiophenes thin films: influence of kinetic energy on submonolayer formation and device performance, Matteo Tonezzer
 02/12/09, Single graphene sheets on various substrates, Petra Rudolf
Support educational activity and teaching
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Graduate Student: GIACOMO SCIUTO
Title of the thesis: Innovative LTHS elements design based on nanotechnologies
Supervisor: Roberto Muscia
Tutors (if any):
Research Activity
In the first phase of the first year of activity the technical background on PCM has been provided
through a detailed literature review. Later, some Matlab numerical models have been developed
(using Enthalpy method) for modelling the phase change (liquid/solid and vice versa) and the
models have been tested on 1-D and 2-D geometries. Two kinds of discretizations have been used:
Finite Differences Method and Finite Volume Method.
A cylindrical heat exchanger that involves PCM has been projected and an improvement of thermal
performances has been noticed by using a macro-doped mixture called CENG-PCM. With the aim
of making the latent heat exchanger more suitable for commercial uses and more modular, a new
topologic solution has been investigated: the panel model. The “energetic” design has been done for
the unity of the system (the panel). It has been noticed that, the path of the water pipe has a very
important role in the efficiency of the device and the topological CFD optimization has started (now
running).
In the last phase of the year activity, the effect of nano-doping in PCM (paraffin wax) has been
investigated. Carbon Nano Tubes (Single Walled, diameter 1.3nm) has been selected as good
doping material and a DSC analysis has been brought to highlight the alteration of latent heat:
several mixtures have been tested (e.g. 0.1%-0.2%-0.5%...till 2%) and alteration (till 7%) on the
latent heat have been noticed, in agreement with results of Shadab et al. in Journal of Applied
Physics 103.
Objectives for the following year (if applicable)
In the next year:
 finish CFD optimization for panel latent heat exchanger (CENG-PCM);
 define a database of thermo-physical properties of PCMs (nano)doped
 design a new heat exchanger that implies nano-doped-PCM
Once defined the best doped PCM, the energetic design/optimization of the prototype will be done.
Publications on scientific journals (printed or in press)
Waiting for a response – R.Muscia, G.Sciuto, F. Zuddas – On the usage of PCM elements within
asphalt concrete to avoid ice formation on roadways. International Journal of Solar Energy
Publications/abstracts in conferences/congresses (national or international)
G.Sciuto, R.Muscia – Multiobjective optimization of intracoronary stents by genetic algorithm.
13th international research/expert conference, Trends in the development of machinery and
associated technology, TMT 2009, Hammameth, Tunisia.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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R.Muscia, G.Sciuto – A method for performing quality comparisons of Rzeppa joints. 13th
international research/expert conference, Trends in the development of machinery and associated
technology, TMT 2009, Hammameth, Tunisia.
Participation to conferences (as speaker)
R.Muscia, G.Sciuto - Analisi di fattibilità concettuale di un dispositivo per la propulsione navale
senza l’uso di eliche. Mathematica User Meeting, 2009, Padova Italia.
Educational Activity
Classes followed (date, course, professor, type of course):
Advanced Spectroscopy (11-15 May): M.Kiskinova- Course by ELETTRA organized by PhD
school in Nanotechnology
Modellazione Molecolare (October - Dicember 2009): S. Pricl – Master Course by Chemical and
Material Engineering. University of Trieste.
Congressi, seminari, corsi avanzati e altre attività didattiche passive:
Final Conference 2008 (January 2009 – 20 hours)– PhD School in Nanotechnology
Virtual Privat Network – (1 Hour) - Fermeglia
Active vibration control (March – 2 hours) – Dip. Ing. Meccanica
Nanochallenge and Polymerchallenge (April – 1 hours) – Talarico
E-spr Workshop (11 May– 4 hours)– Manzan
Chimica Fisica delle Interfasi (15 May – 1.5 hours) – Colombo
COMSOL Workshop (May– 4 hours) – Tozzo
Energie rinnovabili: Europa Oggi (May – 1.5 hours) – V.Prodi et al.
Visita centrale Nucleare Krsko (May – 8 hours) – Dip. Energetica
Smart Energy System (June – 4 hours) – Taccani
Information meeting for doctoral students (June – 3 hours) – SBA
Mathematicha User Meeting (September – 8 hours)
How to write (and manage) a research project (October – 5 hours) –AA.VV.
Support educational activity and teaching
DSC analysis on nano-doped PCM at the Pharmacy department at University of Trieste.
Development of numerical code for modeling phase change (FVM,FDM), about 1 month.
Article and book review, about 1.5 months.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Scheda del dottorando Federica Tavano
[email protected] [email protected]
Titolo della tesi: Cell mobility and metastatic spreading: a study on human neoplastic cells
using Optical Tweezer
Supervisore: Dott.ssa Serena Bonin
Tutore: Dott. Dan Cojoc, Prof. Giorgio Stanta
Research activity
The pourpose of this project is a systematic in vitro analysis of the mechanical properties
of different human cell lines from different types of solid tumors, characterized by different
metastatic potential and differentiation levels. In particular we are going to study cell
membrane viscoelasticity and local adesion. A direct correlation exists between an increase
in deformability and progression of the transformed phenotype from a nontumorigenic cell
line into a tumorigenic, metastatic cell line. Mechanisms that generate forces for cell
migration have been qualitatively described, but nowadays there are no systematic studies of
quantitative viscoelastic measurements on human tumor cells. Our research will focus on the
characterization of the increased cellular motility in the malignant cells during the metastatic
process and on the role of biomechanics in the tumor growth.
During the first year a custom optical tweezers setup, enforced with a Lateral Shearing
Interferometer Microscope has been designed and realized. Optical Tweezers will be used
to trap and move functionalized 1,5 μm silica beads on the top of the cell. The cell will be
displaced with a nano-piezoelectric stage to induce tension/compression with controlled
strain. Lateral Shearing Interferometry is a technique which permits to measure at the
nanometric level phase gradients in axial direction. In this way it‟s possible to measure
deformations and movements of the objects with very high precision. We will use this
technique for viscoelastic measurements from the thermal fluctuations of the cell membrane.
During this year part of the cell lines on which I will perform the analysis have been chosen,
spread and preserved: in particular cell lines from breast cancer (SKBR, MCF-7, MDAMB
231) and colorectal adenocarcinoma (HT-29, RKO) have been selected. Experiments of
bead functionalization and bead positioning have been done: first, carboxyl groups and type
IV collagen functionalized beads have been tried. Then, in a second time, fibronectin and
other proteins functionalized beads will be tried.

Plans for the next year
During the second year I will perform systematic mechanical analysis on the cell lines:
an elasticity range will be determined for the analyzed cell lines, characterized by different
metastatic potential and differentiation levels. The following tumor models will be analyzed:
skin melanoma, breast carcinoma and colorectal adenocarcinoma. In particular, for each
solid tumor model least 2 different cell lines will be used, i.e. poorly and intermediated/well
differentiated cells.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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From the same cell lines, membrane and cytoskeletal proteins will be extracted to reveal
possible significative differences at the proteomic level, in order to associate mechanical
analysis results with molecular analysis.
The long term objective is to define possible biomarkers of metastatic diffusion that
could find applications for diagnosis and prognosis of the considered neoplastic lesions.


Pubblicazioni/abstracts in conferenze/congressi (nazionali o internazionali)
1. Poster: “High spatial resolution BDNF delivery by means of laser tweezers to
stimulate hippocampal neurons signalling”; Elisa D‟Este, Gabriele Baj, Paolo
Beuzer, Enrico Ferrari, Federica Tavano, Enrico Tongiorgi, Dan Cojoc; EBSA 2009
 Poster: “High Resolution Drug Delivery by Optical Tweezers Microscopy” Elisa
D‟Este, Gabriele Baj, Paolo Beuzer, Enrico Ferrari, Federica Tavano, Enrico
Tongiorgi, Dan Cojoc; Super and High Resolution Imaging 2009
 Poster: “Optical Tweezers as means to stimulate hippocampal neurons with
BDNF coated beads”; Elisa D‟Este, Gabriele Baj, Paolo Beuzer, Enrico Ferrari,
Federica Tavano, Enrico Tongiorgi, Dan Cojoc; Nanobioviews-2nd international
symposium on Optical Tweezers in life sciences
Attività formativa
Ore di lezione seguite I anno (corso, data/periodo, docente, tipo di corso di laurea)
1. Nanotecnologie e Nanomicroscopie
Docenti: Erik Vesselli(32 ore)/ dott. L.Businaro (24 ore)
Corso di Laurea Specialistica in Biotecnologie Mediche (indirizzo nanotecnologie) SSD
FIS/03
2. Spettroscopie Ottiche Applicate e Analisi di Immagini
Docenti: A.Bonifacio (16 ore)/ dott. A. Accardo (8 ore)
Corso di Laurea Specialistica in Biotecnologie Mediche (indirizzo nanotecnologie)
ING-INF/08
3. Advanced imaging and Spectromicroscopy methods for chemical and structural
characterization of micro-and nanomaterials
Docente: Maya Kiskikova Ph.D Sc.D (15 ore)
4. Ottica e microscopia avanzata:
Docente Dan Cojoc: gennaio-luglio (25 ore)
Practical work during the first year
The biological part of my experimental work (choice, spreading, preservation of the cell
lines….) has been done at the Molecular Histopatology Lab at the ACADEM Department
of University of Trieste. Practice work regarding the design and realization of the Optical
Tweezers and lateral Shearing Interferometer Microscope has been done at the Optical
manipulation Lab at TASC-INFM under the supervision of Dr. Dan Cojoc.
All together practical work largely covered the limit of 60 hours.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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
Congressi, seminari ed incontri organizzati dalla scuola
1. Congresso scuola di dottorato in Nanotecnologie gennaio 2009 (9 ore)
 Seminario, “Modeling reactions at the nanoparticles scale” Prof. Philip
R.Westmoreland.Dept. of Chemical Engineering. University of Massachuttets.
Amherst; 8 maggio 2009
 Seminario CENMAT e presentazione lavoro dottorandi III anno; 26 novembre
2009 (4 ore)

Corsi avanzati seguiti – seminari – altre attività didattiche passive
1.Corso sicurezza laser; Elettra 25 marzo 2009; (4 ore)
2.School: Introduction to optofluidics (40 ore) 1-5 giugno 2009, ICTP
3.Workshop microscopia AFM per campioni biologici, 5 maggio 2009(6 ore)
4.Worksop: “Orion Plus-The Ultimate resolution”, Milano 28 maggio 2009(5 ore)
5.EBSA European Biophysics congress, Genova 11-15 luglio 2009(30 ore)
6.Super and High Resolution Imaging, Lipica 30 settembre-2 ottobre 2009(20 ore)
7.Nanobioviews: second International Symposium on Optical tweezers in Life
Sciences,JPK Berlino,14-15 ottobre 2009(16 ore)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Graduate Student CHIARA ZANUSSO –[email protected]
Title of the thesis: “Nanotechnologies and oncology: pharmacokinetics and pharmacogenomics
to optimize the antitumoral therapies”
Supervisor: prof. Tullio Giraldi
Tutors (if any): dr. Giuseppe Toffoli
Research Activity
This study purpose is to characterize pharmacogenetic markers above all the neurological type
involved in the response and toxicity to the pharmacologic treatment with oxaliplatin in patients
with colorectal cancer. At first literature search has been done to define the genetic polymorphisms
of possible prognostic/predictive significance. Polymorphisms of glutathione S-transferase (GST)
has been identified, transmembrane transporters (ATP-Binding Cassette) that are responsible for the
extrusion of the drug from the cell, and polymorphisms concerning DNA repair genes that codify
for proteins involved in the repair of the damage exerted on nucleic acid from mutagens as
alkylating drugs.
154 patients were enrolled with colorectal cancer (CCR) treated with adjuvant FOLFOX-4 from the
National Cancer Institute of Aviano (CRO) and other centres of Veneto region. At first genomic
DNA was extracted from peripheral blood samples and then the development of analytical methods
was performed. Specifically an innovative use of nanotechnologies in gene sequencing has been
implemented, such as Pyrosequencing, a technique of recent development, that allows to identify
simply and quickly mutations of punctiform type (SNP). Finally statistical analysis of data was
performed to evaluate the relative risk (OR) of developing colorectal cancer by means of casecontrol studies and the role of individual gene variations in making prone the patient to the
development of toxic reactions after chemotherapy with oxaliplatin.
Objectives for the following year (if applicable)
 Study of pharmacoradiogenetic variations in the toxicity and response to chemoradiotherapy in
prostate cancer patients;
 Determine the impact of these polymorphisms in patients with prostate cancer compared with
the general population in order to determine the possible role on carcinogenesis of the prostate
cancer.
Publications/abstracts in conferences/congresses (national or international)
1. “FARMACOGENETICA DELLA CARDIOTOSSICITÀ DA ANTRACICLINE”
Chiara Zanusso and Giuseppe Toffoli,
(long abstract for the conference entitled “Complicanze cardiovascolari in oncologia: ieri ed
oggi. La gestione delle problematiche” at Napoli on March 25-26, 2009)
2. “NANOTECHNOLOGIES AND ONCOLOGY: PHARMACOKINETICS AND
PHARMACOGENOMICS TO OPTIMIZE THE ANTITUMOR THERAPIES”
Chiara Zanusso, Erika Cecchin, Paola Biason, Elena De Mattia, Franca Sartor and Giuseppe
Toffoli
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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(Poster presented at the 2nd Phd workshop 10th/11 September 2009 at Wittenberg)
(Abstract presented at the “Congresso Nazionale della Società Italiana di Chemioterapia”
at Udine on October 14, 2009)
Educational Activity
Periods abroad (date and place)
Classes followed (date, course, professor, type of course)


700 hours of study and deepening of the topics covered by books and review-type paper and /
or electronic from January 1, 2009;
700 laboratory hours at the "Experimental and Clinical Pharmacology" CRO Aviano headed by
dr. Giuseppe Toffoli from January 1, 2009
Conferences, seminars, advanced courses and other didactic activities
“Congresso annuale delle presentazioni dell’attività scientifica dei dottorandi in corso” at
University of Trieste on January 13-14-15, 2009 from 9.00 to 18.30
“Seminario sull'uso della VPN e del Portale” at University of Trieste on March 20, 2009 at
15.00.
“Esame Finale 2009” at University of Trieste on April 8, 2009 at 9.00.
“Modeling reactions at the nanoparticle scale” Prof. Phillip R. Westmoreland Dept of Chemical
Engineering, University of Massachusetts Amherst, at University of Trieste on May 8, 2009 at
15.00.
“Chimica fisica delle interfasi in sistemi nanostrutturati” Dr. Italo Colombo, at University of
Trieste on May 15, 2009 at 11.00.
“Incontri informativi per i dottorandi: orientamento all’utilizzo delle risorse e dei servizi
documentali forniti dal Sistema Bibliotecario di Ateneo; panoramicadelle nuove modalità di
comunicazione scientifica: l’archivio istituzionale di Ateneo OpenstarTs; l’autoarchiviazione
delle tesi di dottorato e cenni sull’accesso aperto alla letteratura di ricerca; procedure di
conferimento al sito login miur e all’anagrafe di Ateneo (Saperi) at University of Trieste on
June 30, 2009 from 9.30 to 12.30.
“Joint workshop 10th/11 September” at Wittenberg with a poster presentation “Nanotechnologies
and oncology: pharmacokinetics and pharmacogenomics to optimize the antitumor therapies”.
“How to write (and manage) a research project” at University of Trieste on October 16, 2009
from 14.00 to 19.00. Lecturers: Cecilia Blasetti (Elettra), Valter Sergo (UNITS), Maurizio
Fermeglia (UNITS).
Seminars (at CRO Aviano):
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“Dal Focus 2009: aggiornamenti sul carcinoma mammario” on March 25, 2009 from 14.30 to
18.30.
“Roles of small RNAs in genome defense” on May 7, 2009 at 11.00.
“Seminari in Oncologia al C.R.O. di Aviano” on May 28 and June 4-11-18, 2009 from 16.00 to
19.00.
“Matrix revolutions: Perlecan domain V as a novel cancer and stroke therapy” G. Bix - Texas
A&M College of Medicine- USA on September 14, 2009 from 14.30 to 15.30.
“Invasion and Metastasis in Breast Cancer” on November 23, 2009 from 14.30 to 15.30.
Conferences:
“Congresso Nazionale della Società Italiana di Chemioterapia”, Udine on October 14, 2009
from 9.00 to 13.00.
Courses (at CRO of Aviano):
“Corso di lingua inglese presso lo Shenker Institute di Pordenone”: from January to July 2009,
20 hours of lessons with final certificate.
“RefWorks: creare DataBase Bibliografici personali” on May 7, 2009 from 14.00 to 18.00.
“REFERENCE MANAGER: uno strumento straordinario per ottimizzare la gestione delle
bibliografie” on May 19 and 26, 2009 from 14.00 to 17.30.
“BLSD - Corso teorico pratico di Basic Life Support and Defibrillation” on September 22,
2009 from 8.45 to 18.00.
“L'informazione scientifica in un IRCCS oncologico: formazione all'uso delle risorse
informative rese disponibili dalla Biblioteca del CRO, Bibliosan” on September 29, 2009 from
9.00 to 13.15 .
Support educational activity and teaching

two hours of class teaching of Pharmacology Oncology of the degree course in “Biotecnologie
Mediche”, University of Trieste on January 2009:
“Gli inibitori della farnesiltransferasi” e “Il proteasoma: ubiquitina e Bortezomib”
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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4. Progetti Dottorandi del 25 ciclo:
Sono inclusi in questa parte del documento i progetti scientifici dei seguenti dottorandi del 25 cilco:
AMBROSINI Stefano, BIDOGGIA Silvia, CHEN Yan Xin, FRAGKOU Effrosyni, GANAU
Mario, GIORGIS Valentina, LUCAFO' Marianna, MARSICH Lucia, PERONIO Angelo,
SAMMITO Davide.
+
Dottorando STEFANO AMBROSINI
Email: [email protected]
Laboratorio di lavoro: IOM CNR, Laboratorio TASC
Titolo della tesi: Sintesi di nanostrutture di semiconduttori e loro studio mediante luce di
sincrotrone.
Supervisore: dr. Silvia Rubini
Attività di ricerca prevista
Stato dell’arte e motivazioni
I nanofili di materiali semiconduttori sono considerati nanostrutture molto promettenti per futuri
sviluppi nanotecnologici nel campo della sensoristica, della conversione fotovoltaica e potrebbero
trovare applicazioni nella miniaturizzazione dei dispositivi in microelettronica. In alcune di tali
applicazioni si sfrutta l‟alto rapporto superficie / volume dei nanofili, mentre in altre si utilizza su
scala nanometrica la loro natura di semiconduttori.
L‟uso di nanofili semiconduttori per gli scopi citati necessita di una profonda conoscenza delle loro
caratteristiche elettroniche e cristalline, e di un accurato controllo di esse in fase di crescita. In
questo quadro di riferimento si inserisce la ricerca da svolgersi durante questo dottorato.
Molti metodi sono stati proposti in letteratura per indurre la crescita di nanofili. La maggior parte di
tali metodi utilizza la pre-deposizione di un catalizzatore [1](e.g, Au od altri metalli) sul substrato o
processi litografici [2] per modificare il substrato in modo da creare una serie di centri di
nucleazione discreti. La possibile incorporazione di atomi di catalizzatore nei nanofili o la creazione
di difetti durante i processi litografici sono tuttavia potenziali problemi che abbassano la qualità
globale delle nanostrutture e ne minano l‟impiego in campo tecnologico.
Le proprietà dei nanofili dipendono strettamente dalla tecnica utilizzata per la loro sintesi, pertanto
uno studio capillare dei processi di crescita e delle relazioni tra essi e le proprietà dei nanofili
costituisce premessa fondamentale per l‟utilizzo delle nanostrutture in dispositivi.
Le tecniche di caratterizzazione basate sulla luce di sincrotrone possono fornire informazioni
importanti sulla struttura cristallina dei nanofili, la loro struttura elettronica, l‟incorporazione di
drogante o impurezze la loro reattività superficiale anche con elevata risoluzione spaziale [3], e
possono quindi dare contributi significativi alla comprensione delle proprietà delle nanostrutture e
indirizzare il disegno di dispositivi innovativi.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
89
[1] V. G. Dubrovskii et al. Phys. Rev B 71, 205325 2005
[2] A. Fontcuberta i Morral, appl. Phys. Lett. 92, 063112 �2008�
[3] A. Koilmakov et al, ACSNano 2, 1993 (2008).
Obbiettivi da raggiungere nei tre anni
oComprensione delle relazioni esistenti tra condizioni di crescita e proprietà dei nanofili.
oAffinamento del controllo delle condizioni di crescita per ottenere la maggiore flessibilità
possibile nelle proprietà dei nanofili.
oSviluppo di prototipi di dispositivi impieganti i nanofili sintetizzati.
Obbiettivi da raggiungere per il primo anno
oAffinamento della tecnica di crescita senza catalizzatore e comprensione dei meccanismi di
crescita coinvolti. Comprensione della dipendenza delle proprietà dei nanofili prodotti dal
substrato impiegato e dalle condizioni di crescita (pressione dei materiali costituenti,
temperatura di crescita) nel caso di crescita senza catalizzatore.
oValutazione della possibilità di utilizzo della stessa tecnica per la crescita di nanofili di materiali
diversi dal GaAs.
oConoscenza approfondita delle tecniche di indagine che utilizzano la luce di sincrotrone e delle
loro potenzialità per l‟analisi di nanofili semiconduttori.
Progetto di ricerca
Sintesi di nanofili.
Dopo aver messo a punto un‟innovativa tecnica per la sintesi di nanofili di GaAs tramite epitassia
da fasci molecolari (MBE) senza l‟utilizzo di catalizzatore, durante il dottorato si valuterà la
possibilità di applicare questa stessa tecnica a nanofili di altro materiale. I nanofili così prodotti
potranno essere confrontati con quelli cresciuti utilizzando catalizzatore metallico (Mn, Au, Fe, …).
Un interesse particolare sarà rivolto alla possibilità di incorporare in maniera controllata impurezze
(droganti) per modificare le proprietà elettriche delle nano strutture, e alla dipendenza del
drogaggio così ottenuto dal catalizzatore utilizzato.
Altro aspetto importante sarà la realizzazione di nanofili composti da regioni di materiali diversi
(etero strutture), o con proprietà elettriche diverse (p.es. giunzioni p-n).
Caratterizzazione dei nanofili - metodi di laboratorio.
La morfologia dei nanofili sarà studiata in maniera sistematica tramite microscopia elettronica a
scansione (SEM), le loro proprietà strutturali tramite microscopia elettronica in trasmissione (TEM)
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grazia alla collaborazione con il dott. V. Grillo (IN –CNR, Modena) e spettroscopia Raman e microRaman, e le loro proprietà ottiche tramite spettroscopia di fotoluminescenza (PL) anche risolta
spazialmente (micro-PL).
Verranno realizzate misure di trasporto elettrico atte a misurare la conducibilità delle nanostrutture,
drogate e non e valutare la densità di portatori di carica.
Caratterizzazione dei nanofili - luce di sincrotrone.
L‟attività di caratterizzazione delle nanostrutture con luce di sincrotrone comprenderà turni di
misura su linee di luce che utilizzano sia tecniche di microscopia che di spettroscopia e di
diffrazione. L‟interesse sarà posto sulla struttura cristallina delle nano strutture (diffrazione),
l‟incorporazione e la determinazione della configurazione atomica locale di droganti o impurezze
provenienti dal catalizzatore (EXAFS) , la reattività superficiale dei nanofili (fotoemissione) in
relazione alle loro proprietà di trasporto in vista di possibili applicazioni come sensori.
Gli esperimenti verranno realizzati in maniera più consistente a partire dal secondo anno, ed il loro
programma sarà determinato nel corso del lavoro, in collaborazione con i responsabili delle linee di
luce.
Nel caso di misure sensibili alla superficie, le nanostrutture cresciute presso il laboratorio TASC
verranno trasferite alle camere di analisi delle beamline di Elettra protette da uno strato di arsenico
che, depositato nella camera di crescita, verrà rimosso termicamente prima delle misure. Questa
tecnica che si è dimostrata molto efficace nell‟impedire l‟esposizione all‟aria delle nanostrutture.
Attività didattica
Formazione:
oCorso “Applicazioni della luce di sincrotrone” tenuto dal prof. Paolucci nel curriculum di laurea
magistralis di Fisica dello Stato Solido all‟università di Trieste.
o“School on Synchrotron and Free-Electron-Laser Sources and their Multidisciplinary ICTP,
Trieste 26-4/7-5 2010.
Insegnamento:
o Intervento di ore di lezione 4 presso la scuola media G. Luci di Muggia (TS) a proposito delle
possibilità di impiego in campo fotovoltaico delle nanostrutture.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Graduate Student: Silvia Bidoggia
Email: [email protected]
Laboratory: Lab. 345 Dip. Sc. Chimiche, Edificio C11 Tel. 040-5583926
Title of the thesis: Gold nanoparticles as carriers for drug delivery applications.
Supervisor: Prof. Pasquato Lucia
Research Activity foreseen
State of the art and motivations:
Recently gold nanoparticles (AuNPs) protected by organic monolayers were studied in a variety of
science sectors from physics to material science, from medicine to biology.1
Gold nanoparticles have captured researchers „attention because of their peculiar properties, their
high stability compared to other metal nanoparticles, solubility in organic solutions and water, easy
funzionalization and also because of their biological compatibility. Numerous studies have
demonstrated that they are good attractive candidates for delivery of various payloads into their
targets. This payloads could be small drag molecules or large biomolecules, like proteins, DNA,
RNA. These could be covalently conjugated to AuNPs via cleavable linkers, or baunded to AuNPs
through weak interactions conjugation without structural modification of the drag playload.
Prerequisites of drug delivery systems are the efficient release and the improvement of solubility, in
vivo stability and biodistribution of these therapeutic agents. Gold nanoparticles are suitable as drag
delivery systems because of their unique chemical and physical properties:
-the gold core is essentially inert, non-toxic and biocompatible;
-the syntheses is easy and provide monodisperse nanoparticles with core sizes ranging from 1 to
150 nm;
-they have high surface-area-to-volume ratio and as a result an high density of ligands can be
appended for targeting or drug-loading purposes;
-they can be ready funzionalizated with a large variety of ligands that play as linkers for
therapeutic agents or as labels to monitor the NPs fate or as targets for recognition.
In recent years significant efforts have been devoted to the syntheses of monodisperse nanoparticles
with controlled size and passivated with thiols (thiols ligands strongly bind gold due to the soft
character of both Au and S). Brust and Schiffrin2 have introduced the first method for AuNPs
synthesis, published in 1994, allowing a facile synthesis of thermally stable and air-stable AuNPs
with reduced dispersity and controlled size. This method AuCl4- is transferred from water to toluene
using tetraoctylammonium bromide as the phase-transfer reagent and reduced by NaBH4 in the
presence of alkenethiols. The core size of the particles can be varied from 1.5 nm to 6 nm by
varying the thiol-gold stoichiometry, the temperature and the rate of reducent addition. More
recently were introduced new methodologies for the synthesis of AuNPs passivated with an organic
monolayer and soluble in water.3
To enhance the functional versatility of AuNPs, mixed monolayer-protected AuNPs can be
synthesized directly with proper ligands or through post synthetic functionalization. The most
commonly used method for creation of mixed monolayer-protected AuNPs is through use of the
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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place exchange reaction first introduced by Murry.4 In this protocol, external thiols displace the
existing ligands of AuNPs in an equilibrium process.
Drugs linked covalently could be released by the presence of thiols (e.g. gluthatione) into the cell. 5
Rotello developed a nanoparticle-based delivery system featuring glutathione release6. The particles
featured a mixed monolayer composed of cationic ligands (TTMA) and fluorogenic ligands
(HSBDP). The cationic surface of the particle facilitated their penetration through cell membranes
and the release was triggered by intracellular glutathione (GSH). The fluorescence of the Bodipy
moiety of the HSBDP was silent when linked to the particle, but in presence of high concentrations
of GSH, the Bodyp was released and that fluorescence could be detected. Using these particles the
release of the drug related to the concentration of GSH into the cell could be monitorored.
Non-covalent incorporation of drug into AuNPs monolayers provides an alternative delivery
strategy that allows direct use of unmodified drugs. Drug encapsulation with AuNPs relies on the
use of ligands that generate a hydrophobic pocket into the monolayer. Pasquato demonstrated the
encapsulation of radical probes in AuNPs monolayers, using ESR spectroscopy to monitor the
partition of lipophilic probes between a monolayer of AuNPs and bulk water.7 It was demonstrated
also that smaller nanoparticles featuring more strongly radial monolayers favor guest encapsulation.
Another important field is that of perfluorinated amphiphile compounds. They have the
characteristic to be biocompatible and soluble in polar solvents. This aspects could be taken into
account for applications in material science and biomedical field. In fact the use of perfluorinated
amphiphilic compounds may open the way to new biomedical applications, for example in the field
of antioxidant carriers or in the development of multicompartement nanoparticles with the capacity
to store and release active molecules. In recent years in the laboratory in which I‟ll carry out my
research activity has investigated gold nanoparticles protected by a monolayer of perfluorinated
amphiphilic tiolates (HS-F8-PEG) characterized by a perfluorinated portion and a poly(ethylene
glycol) unit, or a mixed monolayer composed of HS-F8-PEG and HS-C8-TEG.9 ESR experiments
on this mixed monolayers suggest the formation of patches in which the hydrophobic probe
interacts preferentially with the perfluorinated portions.10 These are very interesting systems and
they will open the way toward the possibility to conjugated into the NPs two different active
molecules with different characteristics and carry and release them into cells.
1.
G. Schmid, Ed., Nanoparticles. From Theory to Application, Weinheim: Wiley-VCH, 2004. G. Schmid, Chem.
Rev. 1992, 92, 1709–1727. M. C. Daniel, D. Astruc, Chem. Rev. 2004, 104, 293–346. C. M. Niemeyer, C. A.
Mirkin, Eds., Nanobiotechnology,Weinheim:Wiley-VCH, 2004; (b) C. A. Mirkin, C. M. Niemeyer, Eds.,
Nanobiotechnology II, Weinheim: Wiley-VCH, 2007. V. Rotello, Ed., Nanoparticles. Building Blocks for
Nanotechnology, New York: Kluwer Academic/Plenum, 2004. N. L. Rosi, C. A. Mirkin, Chem. Rev. 2005, 105,
1547–1562. C.-C. You, A. Chompoosor, V. M. Rotello, Nano Today 2007, 2, 34–43. G. F. Paciotti, L. Myer,
D.Weinreich, D. Goia, N. Pavel, R. E. McLaughlin, L. Tamarkin, Drug Deliv. 2004, 11, 169–183.
2.
M. Brust, M. Walker, D. Bethell, D. J. Schiffrin, R. Whyman, J. Chem. Soc., Chem. Commun. 1994, 801–802.
3.
P. Pengo, S. Polizzi, M. Battagliarin, L. Pasquato, P. Scrimin, J. Mater. Chem. 2003, 13, 2471–2478. E. E. Foos,
A.W. Snow, M. E. Twigg, M. G. Ancona, Chem. Mater. 2002, 14, 2401–2408. A. G. Kanaras, F. S. Kamounah, K.
Schaumburg, C. J. Kiely, M. Brust, Chem. Commun. 2002, 2294–2295.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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4.
M. J. Hostetler, S. J. Green, J. J. Stockes, R. W. Murray, J. Am. Chem. Soc. 1996, 118, 4212–4213; R. S. Ingram,
M. J. Hostetler, R. W. Murray, J. Am. Chem. Soc. 1997, 119, 9175–9178.
5.
(a) Hong, R.; Han, G.; Fernandez, J. M.; Kim, B. J.; Forbes, N. S.; Rotello, V. M. J. Am. Chem. Soc. 2006, 128,
1078–1079. (b) Paciotti, G. F.; Kingston, D. G. I.; Tamarkin, L. Drug DeVelop. Res. 2006, 67, 47–54. (c) Gibson,
J. D.; Khanal, B. P.; Zubarev, E. R. J. Am. Chem. Soc. 2007, 129, 11653–11661. (d) Ghosh, P.; Han, G.; De, M.;
Kim, C. K.; Rotello, V. M. AdV. Drug DeliVery ReV. 2008, 60, 1307–1315. (e) Cheng, Y.; Samia, A. C.; Meyers,
J. D.; Panagopoulos, I.; Fei, B. W.; Burda, C. J. Am. Chem. Soc. 2008, 130, 10643–10647.
6.
C. K. Kim, P. Ghosh, C. Pagliuca, Z.-J. Zhu, S. Menichetti, V. M. Rotello, J. Am. Chem. Soc. 2009, 131, 13601361.
7.
M. Lucarini, P. Franchi, G. F. Pedulli, P. Pengo, P. Scrimin, L. Pasquato, J. Am. Chem. Soc., 2004, 126, 9326-9329.
Lucarini, M.; Franchi, P.; Pedulli, G. F.; Gentilini, C.; Polizzi, S.; Pengo, P.; Scrimin, P.; Pasquato, L. J. Am. Chem.
Soc. 2005, 127, 16384–16385.
9.
C. Gentilini, F. Evangelista, P. Rudolf, P. Franchi, M. Lucarini, L. Pasquato, J. Am. Chem. Soc. 2008, 130, 1567815682.
10. C. Gentilini, P. Franchi, E. Mileo, S. Polizzi, M. Lucarini, L. Pasquato, Angew. Chem. Int. Ed. 2009, 47, 30603064.
Objectives for the three years
The main objectives of the project are:
-Acquisition of the knowledges about syntheses, characterization, drug delivery applications of
AuNPs.
-Preparation and characterization of different kinds of AuNPs and study their application as
drug delivery systems.
Objectives for the first year
For the first year the most important objectives are:
-Bibliographic resuming and lectures to improve knowledges about self assembled monolayers.
-Syntheses and characterization of AuNPs passivated with the thiol HS-fluoro-PEG.
-Syntheses and characterization of mixed monolayers with fluorinated and alkylthiols.
-Investigation of the binding ability of small drugs inside the monolayers of the prepared NPs.
Research project
-Bibliographic resuming about fluorinated nanoparticles, polymers and dendrimers focusing the
attention on the methods of characterization of this kind of materials and their applications
in biomedical field.
-Syntheses of HS-fluoro-PEG,
-Study of the syntheses of nanoparticles protected by fluorinated thiols of different size.
-Characterization of this NPs with NMR and UV-Vis spectroscopy, TGA, TEM, XPS and EPR
experiments.
-Syntheses of mixed monolayers protected NPs and characterization also with methodologies
different then EPR as STM, IR, chemical reactivity.
Educational activity
During the first year I‟ll carry out the following educational activity:
-Lectures: Proprietà fisiche dei materiali (prof. Franciosi, 48 hours); Materiali organici (prof.
Pasquato, 48 hours)
-Laboratory support (Laboratorio di chimica organica I, 40 hours)
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Graduate Student: Chen Yan-xin
Laboratory: Department of Chemical Sciences (University of Trieste) and National Research
Council (CNR) – Institute of Chemistry of OrganoMetallic Compounds (ICCOM) (Florence and
associated Research Unit of the Department of Chemical Sciences - University of Trieste)
Title of the Thesis: Nanostructured Titanium Dioxide Based Materials for Photocatalysis and
Phototelectrocatalysis
Supervisor: Prof. Paolo Fornasiero
Tutors: Claudio Bianchini, Alessandro Lavacchi
State of the Art and Motivations
Titania NTs, are effective support materials for a variety of noble metal-based catalysts. Indeed,
the physical and chemical properties of nanoscaled TiO2 (crystal structure, morphology, surface
area) may increase the dispersion of active materials on the support, resulting in an high active
surface area and, in turn, in an enhanced catalytic activity. In addition, cooperative effects for
platinum catalysts on TiO2 nanotubes have been observed, and attributed to a change of the
electronic properties of dispersed Pt. These findings well justify for the use of Pd and Pt-doped
titania in photochemical devices. All the above, in conjunction with the remarkable stability of TiO2
in both acidic and alkaline solutions, suggest that TiO2 NTs may be excellent candidates as support
materials for engineering metal catalyzed materials for solar production of hydrogen from aqueous
solutions of renewable alcohols. The application of TiO2 NTs as a support for Pd nanoparticles for
the electro-oxidation of alcohols have also been reported
Figure 1: TiO2 Ordered NanoTube Array (ONTA)
Alcohols are easily produced from biomasses and have an enormous potential in the context of the
“green chemistry”. The possibility of their extensive use for the sustainable chemistry depends
critically on the availability of efficient catalysts.
In this project, we plan to use metal TiO2 NTs, to develop chemo-electro-catalytic materials for the
concomitant production of hydrogen and carboxylic acids from aqueous solutions of renewable
alcohols (ethanol, ethylene glycol and glycerol, mostly) and for the use in photo-activated fuel cells.
This research will imply the manufacture of electrodes with improved characteristics, also in terms
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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of efficiency, chemical and mechanical stability. The deposition of noble and non-noble metal
nanoparticles, and their mixtures thereof, onto nanostructured titania is expected to afford highly
innovative, multipurpose chemo-photo-catalysts suitable for the present target.
Objectives for the three years
Development, fundamental and functional characterization of a variety of photocatalysts and
photoelectrocatalyst for the application in photoreforming, photoelectrolysis and photoactivated fuel
cells.
Objective for the first year
Literature analysis for the definition of the state of the art as a basic reference for benchmarking;
definition of the protocols for both the fundamental and functional characterization of the materials
that will be synthesized; selection trough experimental trials of the best synthetic routes for the
titanium nanostructures.
Research project
Activity 1.1: State of the Art
The state of the art for photocatalysts and photoelectrocatalysts will be critically reviewed. The
obtained data will be used to select systems and synthetic strategies.
Task 1.1: Literature Analysis
A perusal of the existing literature will be performed. The main purposes are a clear definition of
the state of the art and the extraction of the most relevant functional data on the existing systems for
the benchmarking.
Task 1.2: Selection of the Systems
The selection of the systems to be investigated will be performed according to the data provided by
the literature examination. Both the materials and the specific reaction will be selected on the basis
the potential scientific and technological impact.
Activity 2: Synthesis of the Titanium Nanostructures
Titanium dioxide will be synthesized by both electrochemical and chemical methods for the
production of both nanotube arrays and nanostructured carbon supported powders.
Task 2.1: Electrochemical Methods
Anodization of titanium metal substrates will be employed for the synthesis of nanotube arrays with
variable nanotube diameter and length. The anodization will be followed by heat treatment in order
to favour the formation of the photoactive phases.
Task 2.2: Chemical Methods
The chemical synthesis of titanium dioxide nanostructured powders both unsupported and carbon
supported will be performed. The possibility of doping the Titania will also be explored by the
adding boron, nitrogen and eventually other transition metal oxides. The carbon supported materials
developed in this task will be employed as anodes for the photoactivated fuel cells.
Activity 3: Catalyst Insertion
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The addition of catalyst with proved activity toward the systems selected in the task 1.2 will be
performed via both electrochemical and chemical approaches. This activity will provide the
materials which will be tested in the activity 4.
Task 3.1 Electrochemical Methods
Electrodeposition techniques will be employed for adding metallic nanoparticles to the catalysts
developed in the tasks 2.1 and 2.2. Deposition conditions will be varied to tailor the particle
characteristic e.g. composition, structure and morphology for the best catalytic performances.
Task 3.2 Chemical Methods
Existing “electroless” methods will be adapted to the deposition of nanostructured metals on the
materials developed in the tasks 2.1 and 2.2.
Activity 4: Characterization
The characterization will consist in the investigation of the catalysts basic properties (structure,
morphology, composition, basic electrochemistry) and functional performances.
This activity will conclude the project with a benchmarking of the produced materials with the state
of the art and a summary of the main findings of the three years of work.
Task 4.1: Basic Characterization
The fundamental characterization will consist in the definition of the basic properties of the
materials. The composition, the structure and the morphology will be investigated as well as the
composition. Half cell electrochemistry of the materials designed photoelectrocatalysts will also be
performed.
Task 4.2: Functional Characterization
The functional performances will be evaluated under the operating conditions. The selectivity, the
conversion yield and the energy efficiency will be determined. A special focus will be on the time
stability of the produced catalysts. Functional data will be the basis for the benchmarking which
will be the object of the task 4.3.
Task 4.3: Benchmarking and Conclusion
The data derived from task 4.2 will be used for the benchmarking with the state of the art materials.
A summary of the main research conclusions of the three years of research will be delivered at
month 36.
Educational Activity
The educational activity of the first year will mainly consists in the examination of the existing
literature on the ONTA. A special focus will be on the reading of the book “TiO 2 nanotube Arrays:
Synthesis, properties and Applications” by C.A. Grimes and G.K. Mor edited by Springer in 2009.
The activity will be also integrated by the seminars which will be organized by the Istituto per la
Chimica dei Composti OrganoMetallici (ICCOM).
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Gantt Chart
M 1-3
Activity
M 4-6
M 7-9
M
10- M
13- M
12
15
18
16-
M 19- M
21
24
22- M
25- M
28- M
31- M
27
30
33
36
1
State of the Art
Task
1.1
Literature analysis
Task
Selection
1.2
of
the
Systems
Activity
2
TiO2
Nanostructure
Synthesis
Task
2.1
Electrochemical
Methods
Task
2.2
Chemical Methods
Activity
3
Catalyst Insertion
Task
3.1
Electrochemical
Methods
Task
3.2
Chemical Methods
Activity
4
Characterization
Task
4.1
Basic
Characterization
Task
4.2
Functional
Characterization
Task
4.3
Benchmarking and
Conclusion
Figure 2: Gantt Chart
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34-
Doctoral School in Nanotechnologies - XXV Cycle
Doctoral candidate's schedule: EFFROSYNI (FENIA) FRAGKOU
Email: [email protected]
Laboratories: Molecular Histopathology, ACADEM Department, University Hospital of
Cattinara (Trieste) / Elettra Synchrotron Light Laboratory, Centre for Biomolecular
Medicine (CBM) & International Center for Genetic Engineering and Biotechnology
(ICGEB), AREA Science Park (Padriciano-Basovizza, Trieste)
Thesis title: Nanobiotechnologies for Dermatological Applications
Supervisor: Prof. Giorgio Stanta
Tutor: Dr. Serena Bonin
Anticipated research activity
State of the art and motivations
During my PhD period, my purpose is to develop and validate new protocols, pointing to
provide innovative nanotechnological applications in the fields of dermatological diagnosis, with
special concern on skin melanoma.
Malignant melanoma is due to an uncontrolled growth of melanocytes and is the most lethal
form of skin cancer, as it can rapidly spread to the lymph system and internal organs with poor
prognosis. Since surgical treatment is the golden therapy for melanoma, an early diagnosis is vital.
Aim of study:
This project will focus on the nanotechnological applications of tools for dermatological
diagnosis. In particular in the first part of my project I will focus on the search of new biomarkers
for melanoma and for multiple targets imaging I will try to apply to Archive Tissues the technique
of quantum dots.
A second field of interest regards the positive effects of HSPs (Heat shock proteins) on
damaged dermal cells: apoptosis of altered human fibroblasts and cell recovery. Such beneficial
effects could possibly apply either to UV- and chronologically-altered fibroblasts, or to pathological
biostructures including melanoma cells.
Materials and methods:
In the first step of my project I will use tissue microarrays in order to insert on one slide
multiple melanoma clinical specimens. In this first phase I will use mainly Immunohistochemistry
(IHC) to test several biomarkers in a huge amount of clinical specimens.
Later on I will set up the method to transfer quantum dots based imaging to archive tissues.
This part will be subdivided into two steps: the set up of the method using FFPE tissues (FormalinFixed Paraffin Embedded tissues), commonly used for histological diagnosis and the second one
dedicated to the actual application of the technique to the clinical specimens.
Objectives
to be reached in the three years
Application of nanotechnological tools to the dermatological diagnosis.
Objectives to be reached in the first year
During the first year of my PhD project I will focus on the search of new biomarkers for
skin melanoma. To reach this aim I will create a TMA (Tissue Microarray) in order to
analyse a huge amount of clinical samples in one slide. Later, multiple IHC markers will be
studied on this type of arrays in order to select the ones able to diagnose and prognosticate
skin melanomas.
The successive step will be the application of quantum dots technique to archive tissues in
order to obtain multiple imaging.
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Graduate School of Nanotechnology, University of Trieste
Graduate Student: Dr MARIO GANAU
Email: [email protected]
Laboratory: ELETTRA (Synchrotron Light Laboratory), Trieste; University Hospital
Cattinara, Trieste; and in collaboration with the Institute of Pathophysiology, University of
Ljubljana
Title of thesis: Nanotechnology applications in quantitative neuroscience: proteomics
assessment of astrocyte stressing and neuronal plasticity
Supervisors: Prof. GIACINTO SCOLES and Dr. LOREDANA CASALIS (SISSA-ELETTRA
NANO INNOVATION LAB)
Proposed Tutor: Prof. ROBERT ZOREC (University of Ljubljana)
Research Activity foreseen
State of the art and motivations
Once overlooked as merely supportive elements in the central nervous system (CNS), glial cells
have come in the last decade to the centre stage of the efforts to understand the workings of the
brain (P.G. Haydon, Nature 2001). Indeed, the traditional concept, built up in the 20th century, that
macroglial cells, particularly astrocyte, are multifunctional housekeeping cells of neurons has
largely been reconsidered: beside maintaining the optimal environment for functioning of neurons,
evidence emerged that astrocytes may be involved in the regulation of synaptogenenis, in direct
modulation of synaptic strength in the CNS, and they may have an important role in the regulation
of brain microcirculation (E. Newman Trends Neurosci. 2003).
Moreover, astrocytes play a pivotal role in pathologic conditions, by influencing neighboring cells:
they are implicated in early and late stages of neurodegeneration, in reactive gliosis after brain or
spinal cord injury and even in experimental brain infarction (U. Wilhelmsson et al. J Neurosci.
2004). Nowadays astrocytes are known to release chemical messengers, neurotrophins, eicosanoids
and neuropeptides; they also integrate inputs and have long-range signaling systems that can lead to
release of neurotransmitters at a distance and in turn affect the physiological status of neurons;
nevertheless the underlying mechanism of secretion are still under debate (M. Kreft et al. Ann. N.Y.
Acad. Sci. 2009).
The breakthrough discoveries that showed how astrocytes are dinamic and participate in the crosstalk between glia and neurons came as a result of a series of technical advances: studies in cell
cultures first and brain slice later, fluorescent indicators, patch-clamp recording, microscopy and
finally quantitative imaging techniques. The current limit of knowledge advancement is related to
the high sensitivity required to accurately monitor protein-protein interaction to follow changes in
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cellular pathways due to different kinds of external perturbations. This need is so acute and specific
that the field of protein interactions on a genome wide scale has acquired the name of
Interactomics.
The advent of nanotechnology held the promise to overcome these limitations with the possibility of
measuring protein amounts, in samples consisting of single or few cells, down to a hundred
molecules and sub-nanomolar concentrations and in nano-liter to pico-liter volumes. Therefore
strong advancements in this field of neuroscience are expected and surely will come from the
assessment at a nanolevel of the glial proteomics under experimental condition of stress induced by
external stimuli.
Objectives for the three years
The main goal of this PhD thesis is to explore the astrocyte behaviour under influence of external
stimuli via innovative devices and protocols based on micro/nano-fluidics. This nano-quantitative
approach will eventually enable precise, high throughput and low cost measurements on large,
predetermined diagnostically relevant, subsets of the proteome obtained from very small samples
(down to the single cell level) with potential capability of interactomic analysis.
Accordingly the objectives of the project may be highlighted as follows:
 acquisition of the fundamentals basis for the preparation and the study of astrocytes and
their behaviour in physiologic and pathologic conditions;
 acquisition of the skills needed for the development of a research project;
 acquisition of a broad knowledge in the assessment at a nanolevel of synaptic processes and
neuronal plasticity.
Objectives for the first year
Studying the nano-mechanical response of bio-molecular nano-structures, such as stressed
astrocytes, needs as a first task the realization of a nano-chip device.
Along the first year the main objectives to pursue are:
 introduction to nano-fluidics measurments (to be achieved by personal study and tutorials);
 extensive review of the current knowledge concerning astrocyte‟s exocytosis mechanisms
(to be achieved by personal study and internship at the Ist. of Pathophysiology, Univ of
Ljubiana).
 design a nano-fluidic equivalent of the Wheatstone Bridge (a standard tool for electrical
resistance measurements) to further obtain a quantitative proteome analysis (work to be
carried out at Sincrotrone, Trieste).
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Research project:
Herein is reported a standard Gantt chart for the expected tasks and milestones of the thesis.
Task
Start
End
Mos
2010
Q1
Q2
2011
Q3
Q1
Q2
2012
Q3
Q1
Q2
Design of nano-fluidc device for proteomics
purposes
01/2010
04/2011
16
astrocytes behaviour
04/2010
08/2010
4
Studying principles of nano-fluidics
01/2010
12/2010
12
09/2010
08/2011
12
01/2011
12/2011
12
05/2011
12/2011
8
09/2011
06/2012
12
01/2012
08/2012
8
05/2012
12/2012
8
01/2010
12/2012
36
Studying the current state of the art on
In vitro analysis of astrocytes under stressing conditions
First tests of standard proteomics analysis
of astrocytes in physiologic conditions
Validation of the nano-fluidic device for
proteomics analysis
Standard proteomics analysis of astrocytes
in pathologic conditions
Validation tests of the nano-fluidic device
for proteomic analysis of astrocytes under
physiologic conditions
Validation tests of the nano-fluidic device
for proteomic analysis of astrocytes under
pathologic conditions
Editing of thesis
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Q3
Graduate Student VALENTINA GIORGIS
Email: [email protected]
[email protected]
Laboratory: ELETTRA
Title of the thesis: Development, design and characterization of Metamaterials with negative
refractive index
Supervisor: Filippo Romanato
Tutors (if any):
Research Activity foreseen
State of the art and motivations
The neologism “metamaterial” comes from the union of the words “meta”, meaning “beyond”, and
“material”: this term implies, nowadays, a structured composed material that displays properties
usually not found in natural ones [1].
In 1960s Veselago (Moscow Institute of Physics and Technology) [2] first assumed an isotropic
material having simultaneously negative values of electric permittivity (ε) and magnetic
permeability (μ), having, so, negative refractive index (n): it is, then, possible to refer to this kind of
material as Negative Refractive Index Material (NRI) or Double Negative Media. A negative
refractive index implies several effects: the material exhibits negative reflection at the interface,
negative Doppler shift, an obtuse angle for Čerenkov radiation and the possibility of momentum
reversal. Veselago‟s idea remained pure theoretical for more than 30 years, when Smith and
colleagues (University of California, San Diego) [3] designed and fabricated and artificial
effectively homogeneous structure exhibiting simultaneously negative ε and μ, constituted by
standard metals and dielectrics: metal thin-wires (TW) (negative-ε, positive-μ) and metal split-ring
resonators (SRR) (positive-ε, negative-μ). Smith device works as a NRI material in the microwave
range (GHz range).
One of current researchers aim is to design a metamaterial having its operative frequency in THz
range, that is to say at visible wavelength. Though SSR are easy to analyze structures, there are two
main disadvantages designing and fabricating metamaterial using this geometry: it is difficult to
fabricate nano-metric SSR. To face those problems in the last few years a new geometry has been
introduced: a periodic porous nanostructure called fishnet [4]. A fishnet is a three layers structure: a
dielectric layer (usually silicon oxide) placed between two metallic layers (gold or silver).
During the three-years period of PhD fellowship my intention is to study optical metamaterials and
to fabricate nano-porous fishnet using X-ray and interference lithography techniques.
Bibliography
1.
S. Anantha Ramakrishna, Tomasz M. Grzegorczyk. “Physics and Applications of
Negative Refractive Index Materials”. CRC Press, 2009
2.
V. G. Veselago. “The electrodynamics of substances with simultaneously negative values
of ε and μ”. Sov. Phys. Usp., 10(4): 509–14, 1968
3.
D. R. Smith et al. “Composite medium with simultaneously negative permeability and
permittivity”. Phys. Rev. Lett., 84(18), 4184-4187, 2000
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P.-Y. Chen, S.-C. Wu, “A periodic porous-nanostructured left-handed material slab at
optical frequences ”. DOI 10.1002/mop, 2008
4.
Objectives for the three years





Theoretical overview: study of metamaterials, in particular negative refractive index
ones
Getting acquainted with and understanding of modeling techniques
Designing and nanofabrication of negative refractive index metallic metamaterials (one
dimension, two dimensions, three dimensions)
Optical analysis and characterization of the system
Study of potential applications
Obbiettivi da raggiungere per il primo anno
 Study of metamaterials: physical motivation of refractive index change
 Study of modeling techniques
 Gaining experience in nanofabrication techniques: EBL, X-ray lithography,
electroplating, evaporation
 First attempts of metallic metamaterial nanofabrication and characterization
Research project
First year activities:
 Studying: the aim is to acquire a certain knowledge of physics of metamaterial. This
activity will last for the whole first year at least.
 Design of experimental set-up: improvement of LILIT X-ray lithography beamline and
its facilities.
 Membrane substrate fabrication to be used as X-ray mask substrate;
 Mask pattern design: one of the steps to use X-ray lithography technique is to design and
construct X-ray masks; on this first year one of my tasks is to understand which patterns
to use to fabricate metamaterials, to learn how to design them and to do it using CAD
software.
 EBL lithography: after designing it, the pattern has to be created on the membrane. For
this purpose, electron beam lithography will be performed in collaboration with LANN
laboratory in Mestre.

 Gold film deposition: electroplating and/or evaporation will be employed; with this
step, x-ray mask fabric is complete. This technique will be also used to build
metamaterials after x-ray lithography.
 X-ray lithography: I will work at ELETTRA beam line LILIT
 Interference Lithography: an alternative way to fabbricate fishnet patterned
metamaterials is to use interference lithography technique
 Characterization: after its fabrication, sample will be characterized (ellipsometry)
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December
November
October
September
August
July
June
May
April
March
Activity
February
January
Month
Studying
Beam
line
improvement
Lilit
Goniometric stage for
X-ray lithography
X-ray mask fabrication
X-ray lithography
Interference lithography
Educational activity
I intend to focus my attention on physics of metamaterials, physics of surface plasmons,
nanofabrication techniques and synchrotron application. I will take some courses at university
(“Materiali nanostrutturati” and “Applicazioni della radiazione di sincrotrone”) and integrate with
study of books, articles.
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Graduate Student: MARIANNA LUCAFO’
mail:MARIANNA.LUCAFO’@phd.units.it
Laboratory: Department of life sciences
Title of the thesis: Study of carbon nanostructures as carriers for drugs for cancer
chemotherapy.
Supervisor: Prof. Sava
Tutors:Prof. Zorzet,Dott. Pacor,Dott. Daros
Research Activity foreseen
State of the art and motivations
The use of carbon nanostructures (NCS), such as fullerenes and nanotubes as the biomedical field is
of great interest, for its possible therapeutic applications and for its use diagnostics.
Potential advantages of these compounds are given by a structure which makes their internalization
by the cells easy and by their large surface area and internal volume, which make them eligible as
drug delivery systems for the transportation of drugs [Leshuai W. Zhang et al, 2009; Prato M. et al,
2008].
Currently, several studies are underway on nanoparticles with different size and purity, with the aim
to collect an overview about their toxicological profile and to shed light on their potential side
effects on biological components, cells, tissues and physiological processes [Boczkowski J. &
Lanone S., 2007].
It is well known that carbon nanotubes are insoluble and have a strong tendency to aggregate and
this is a constraining factor for their use in biological systems, therefore a range of strategies have
been developed to overcome these problems, based on chemical modification of their surface, both
through non-covalent binding of amphiphilic molecules such as polymers or lipids, or through
covalent functionalization of different chemical groups [Tasis D. et al, 2006; Kostarelos K. et al,
2009].
The latter strategy, in particular, offers the possibility to bind steadily, for example, molecules with
therapeutic activity, avoiding the risk of being degraded by serum enzymes or other blood
components.
So far in the preclinical studies performed in vivo, functionalized nanotubes have been proposed as
carriers for anticancer drugs selectively conveying them just to the target cells and controlling their
release [Kostarelos K. et al, 2007].
An innovative strategy that allows the specific internalization of the compound involves the binding
of a target molecule to the surface of the nanotubes, such as a ligand for a receptor membrane
[Shanta Dhar et al, 2008] or a variable sequence of a specific antibody.
Although few works have been published, the results of the first studies in vivo appear to be
encouraging for the use of carbon nanotubes: the administration of such drug delivery systems,
could delay tumor growth with minimal side effects [Bhirde A. A. et al, 2009].
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Leshuai W. Zhanga, Jianzhong Yang, Andrew R. Barronb, Nancy A. Monteiro-Riviere, 2009.
Endocytic mechanisms and toxicity of a functionalized fullerene in human cells. Toxicology
Letters, doi: 10.1016/j.toxlet.2009.08.017.
Prato M., Kostarelos, K. & Bianco A., 2008. Functionalized carbon nanotubes in drug design and
discovery. Acc. Chem. Res. 41, 60-68.
Boczkowski J. & Lanone S., 2007. Potential uses of carbon nanotubes in the medical eld: how
worried should patients be? Nanomedicine 2, 407-410.
Tasis D., Tagmatarchis N., Bianco A. & Prato M., 2006. Chemistry of carbon nanotubes. Chem.
Rev. 106, 1105-1136.
Kostarelos K., Bianco A. and Prato M., 2009. Promises, facts and challenges for carbon
nanotubes in imaging and therapeutics. Nature Nanotech. 4, 627-633.
Kostarelos K., et al., 2007. Cellular uptake of functionalized carbon nanotubes is independent of
functional group and cell type. Nature Nanotech. 2, 108-113.
Shanta Dhar, Zhuang L., Jurgen T., Hongjie D., Stephen J. Lippard, 2008. Targeted SingleWall Carbon nanotube-mediated Pt(IV) prodrug delivery using folate as a homing device. J. Am.
Chem. Soc. 130, 11467-11476.
Bhirde, A. A. et al, 2009. Targeted killing of cancer cells in vivo and in vitro with EGF-directed
carbon nanotube-based drug delivery. ACS Nano 3, 307-316.
Objectives for the three years
This project, is aimed to get data and knowledge on the mechanisms of the therapeutic activity and
toxicity of carbon nano-structures, in cancer models.
The purpose of the research project is to:
- Study new nanostructures, prepared in collaboration with the group of Professor Prato,
Department of Pharmaceutical Sciences, University of Trieste;
- Define the toxicological profile of the nanotube or fullerene used as carriers for drugs, in single
cells in vitro and in whole animals in vivo;
- Analyze the mechanism of up-take and release of the nanostructure loaded with model anticancer
drugs such as doxorubicin and cisplatin;
- Validate their activity in vivo in models of target tumors.
Objectives for the one year
The first part of the study will focus on the therapeutic potential of the test drug loaded to the
nanostructure vs the free drug and on the identification of the biological models for preliminary
experiments in vitro. A number of established cell lines will be expose to different nanostructures,
carrying or not a drug model, in order to select the most appropriate nanostructures in terms of
interaction with the cancer cells.
The objectives to be achieved for the first year are:
- identify the nanostructure most suitable as carrier;
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-
evaluate any potential selective cytotoxicity on different tumor cell lines;
characterize the sub-cellular pharmacokinetics of the selected nanostructure;
Research project
Outline of the main activities:
-In vitro selection of the nanostructure and of the drug-associated nanostructure.
The test cell lines selected for this studies are those on which adriamycin and cisplatin are mainly
used in the clinical practice. Human cell lines derived from mammary carcinoma and non small cell
lung cancer, including sub-lines resistant respectively to adriamycin and cisplatin will be obtained
from ATCC or similar certificated structure.
The dose and time of cell challenge dependence will be examined in vitro by means of conventional
tests measuring cell viability and apoptosis.
-In vitro analysis of cell and sub-cellular distribution.
The study will be carried out using the cell line in which we showed respectively high and low
response to the selected nanostructure. Conventional methods of preparation of sub-cellular
fractions will be used and will allow to characterize the component(s) of the cell and of the cell
compartment(s) on which the drug associated nanostructures are concentrated.
The combination of the nanostructure plus cisplatin will be studied by means of atomic absorption
spectroscopy where as the combination of the nanostructure with adriamycin will be studied by
means of conventional fluorescent methodologies including flow cytometry.
-In vivo pharmacokinetics and toxicity.
The study of the in vivo pharmacokinetics of the test nanostructures will be carried on in outbred
mice following conventional pharmacological procedures, e.g. intravenous administration and
identification of the whole blood and plasma clearance and of the renal elimination.
Host and organ toxicity will be examined considering the tipical toxicities known for the test drugs
(adriamycin – cisplatin), by administering the drug-nanostructure at doses compatible with their
antitumor activities.
-In vivo characterization of the antitumor potential of the drug nanostructure.
In vivo studies will be performed using target tumors of human origin, obtained from mammary and
lung tumors. The studies will be done using the animal house facility, subject to approval from the
ethical committee and using boxes for immunosuppressed animals.
Educational activity
For the first year, with role of co-rapporteur of some undergraduate students, I will follow them in
the experimental part of their laboratory experience, and I will be working as a tutor during lessons
for high school lab (LLC).
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ACTIVITY
FIRST YEAR
SECOND YEAR
THIRD YEAR
Preparation of the nanostructures
Preparation of drug bound nanostructures
Selection of the nanostructures
Test of citotoxicity of nanostrucutre-drug
In vitro uptake and distribution
Study of cell pathways of interaction of nanostructures
Analysis of cell resistance overcoming
In vivo pharmacokinetics
In vivo activity on tumors
In vivo toxicity
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Graduate Student: Lucia Marsich
Email: [email protected]
Laboratory: DMRN University of Trieste
Title of the thesis: “Design and synthesis of functionalized metal nanoparticles for bio-analysis
with Surface-Enhanced Raman Scattering (SERS)”
Supervisor: Valter Sergo
Tutors: Alois Bonifacio
Tentative Research plan
State of the art and motivations
Raman spectroscopy is a non-destructive technique that gives qualitative and quantitative
information, since the spectrum is a finger print of each molecule, and the intensity of the signal is
proportional to the concentration.
The Raman scattering signal is typically very weak, and enhancement is required to improve the
spatial resolution of the Raman scattering signal. Surface Enhanced Raman Scattering (SERS) has
been successful in enhancing Raman signals using silver, gold or copper surfaces. Particles
composed of such elements are specifically useful as enhancers of Raman signals, since their
surface plasmons are easily excited by laser light, and generate an electric field that can be
transferred to the adjacent Raman active molecules. This results in an amplification of the Raman
signal.
Due to its advantageous characteristics in terms of selectivity and sensitivity, SERS spectroscopy
has become one of the most incisive analytical methods for chemical and biochemical detection and
analysis [1]. SERS spectroscopy provides structure-specific vibrational spectra of analytes with
extremely high sensitivity, and the high selectivity of the technique is ensured by enhanced
detection of only species adsorbed on noble metal surfaces.
Nanoparticles have found various applications in biomedical research. They are used in drug
delivery, intracellular sensing, diagnosis and treatment of cancer.
For example, a class of biocompatible and nontoxic gold nanoparticles for in vivo tumor targeting
and surface-enhanced Raman detection has been developed [2].
Campbell and colleagues have studied the application of gold nanoshells as a surface- enhanced
Raman platform for intracellular sensing in fibroblast cells by using a near-infrared Raman system
[3] .
Raman nanoparticle probes are used as optical labels for interrogation of physiological and
pathological processes in bioassays, cells, and tissues. Furthermore, their unique emission
signatures are ideal for multiplexing, i.e. simultaneous detection of different chemical species.
Commonly used fluorescent dyes or quantum dots present significant limitations in multiplexing
applications due to the interference from tissue autofluorescence and the overlap of emission peaks.
Nanoparticle probes utilizing SERS emit unique spectral signatures with narrow peaks that are ideal
for multiplexing. Raman emission is normally extremely weak compared to fluorescence, but
enormous signal enhancement scan be created by adsorbing Raman-active molecules onto
nanoparticles [4].
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References:
[1]Chourpa, Franck H. Lei, Pierre Dubois, Michel Manfait, Ganesh D. Sockalingum, “Intracellular
applications of analytical SERS spectroscopy and multispectral imaging” Igor Chem.Soc.Rev.,
2008, 37, 993–1000
[2]Ximei Qian, Xiang-Hong Peng, Dominic O Ansari, Qiqin Yin-Goen, Georgia Z Chen, Dong M
Shin, Lily Yang, Andrew N Young, May D Wang, Shuming Nie, “In vivo tumor targeting and
spectroscopic detection with surface-enhanced Raman nanoparticle tags”, Nature biotechnology
volume 26, number 1, 2008
[3]Michael A. Ochsenku, Phillip R. T. Jess, Helene Stoquert, Kishan Dholakia, and Colin J.
Campbell, “Nanoshells for Surface-Enhanced Raman Spectroscopy in Eukaryotic Cells:
Cellular Response and Sensor Development” CS Nano, 2009, 3 (11), pp 3613–3621
[4]Lutz,Barry R. Dentinger,Claire E., Nguyen,Lienchi N., Sun,Lei; Zhang,Jingwu, Allen,April N.,
Chan,Selena, Knudsen,Beatrice S., “Spectral Analysis of Multiplex Raman Probe Signatures”
ACS Nano, 2008, 2 (11), pp 2306–2314.
Objectives for the three years
Literature survey and identification of potentially suitable substrate for SERS in biological
application
Preparation and evaluation of the characteristics of the chosen substrates
Implementation of the SERS-active substrate with biological sample (blood, cells, tissue,
biological molecules)
Objectives for the first year
The aforementioned objectives for the three years will be limited to the problem of the
detection and quantification of the protein bilirubin.
Research project
For each biological sample (biomolecules, cells and tissues) the following activities will be carried
out:
Synthesis of different kind of SERS-active substrate
Characterization of the nanoparticles, in particular the size and the frequency of the surface
plasmon resonance will be estimate (TEM/SEM, ultraviolet-visible spectrophotometry,
Raman and SERS spectroscopy, dynamic light scattering, zeta-potential);
Evaluation of the effect of the coating on nanoparticles characteristic (TEM/SEM,
ultraviolet-visible spectrophotometry, Raman and SERS spectroscopy, dynamic light
scattering, zeta-potential);
Estimation of the enhancement of the Raman signal due to the presence of the substrate
Study of the interaction between the analyte and the substrate;
Measurement of the sensibility of the system (SERS spectroscopy);
Trend analysis of the intensity of the spectrum as a function of the concentration of the
analyte (SERS spectroscopy);
Construction of calibration curves (SERS spectroscopy);
Measures of biological samples and analysis of the results based on the previous
experiment (SERS spectroscopy).
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Educational activity
Study of books and review articles about spectroscopy
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Graduate Student ANGELO PERONIO
Email: [email protected]
Laboratory: IOM CNR, TASC laboratory – Surface Structure and Reactivity group, STM
laboratory
Title of the thesis: Single-molecule heterogeneous catalysis
Supervisor: prof. Giovanni Comelli
Tutors (if any): dr. Carlo Dri
Research Activity foreseen
State of the art and motivations
During my PhD I will study model systems for gas-solid heterogeneous catalysis, focusing on how
the single molecules of the reactants interact with the catalyst and between each other during
chemical reactions. My intent is to tackle the study of these systems by means of a complementary
experimental and computational approach.
The word “catalysis” indicates the process where a chemical reaction is sped up or even made
possible by the presence of a substance (the catalyst) that is not consumed into the reaction
outcomes. Catalysis has an enormous technological impact: catalytic processes underlie the
production of more than 60% of all the presently available chemicals on the market such as fuels,
fertilizers and fine chemicals. Catalysts are also employed in production and purification of
hydrogen for fuel cells and in the reduction of pollutants from cars and industrial plants.
In gas-solid heterogeneous catalysis, the catalytic activity stems from the alteration of the chemical
properties of the reactants upon adsorption on the catalyst surface. Surface science is one of the
various approaches adopted for the characterization of such systems, where the latter are simplified
as much as possible in order to investigate the single steps of the reaction at the atomic/molecular
level, from the binding of the reactants to the catalyst, to the dynamics of the reaction itself.
Among the available spectroscopic, microscopic and structural techniques, low-temperature
scanning tunnel microscopy (LT-STM) allows to image, measure and control the properties of
single atoms, molecules and molecular complexes on surfaces, for instance by manipulating the
adsorbates on the surface [2], by identifying the chemical nature of single molecules from their
vibrational spectra (STM-IETS) [3], by characterizing the processes induced by the inelastic
tunnelling of electrons through the molecule (action spectroscopy) [4], measuring the local energydensity of electronic states (LDOS) by means of dI/dV spectroscopy [5]. These unique singlemolecule capabilities are crucial for instance to fully understand systems where the reactivity is due
to the formation of a complex among the reactants on the surface [6], or to determine the adsorption
geometries of a disordered system.
The simulation of the measured properties (STM images, electronic and vibrational spectra) from
the electronic structure of the surface obtained by means of density functional theory (DFT)
computations is a fundamental, complementary technique to the experimental investigation. One
can for instance predict how the various adsorbates will appear on the images, and in principle
identify the chemical species present on the surface by comparing the theoretical and experimental
images, making the STM a sub-molecular probe with chemical sensitivity.
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Bibliography:
[1] Recognizing the Best in Innovation: Breakthrough Catalyst, R&D Magazine, September 2005,
p. 20.
[2] A. J. Heinrich, C. P. Lutz, J. A. Gupta, and D. M. Eigler, Molecule Cascades, Science 298, 1381
(2002).
[3] B. C. Stipe, M. A. Rezaei, and W. Ho, Single-Molecule Vibrational Spectroscopy and
Microscopy, Science 280, 1732 (1998)
[4] Y. Sainoo, Y. Kim, T. Okawa, T. Komeda, H. Shigekawa, and M. Kawai, Excitation of
Molecular Vibrational Modes with Inelastic Scanning Tunneling Microscopy Processes:
Examination through Action Spectra of cis-2-Butene on Pd(110), Physical Review Letters 95,
246102 (2005)
[5] R. Temirov, S. Soutbach, A. Lucian and F.S. Tautz, Free-electron-like dispersion in an organic
monolayer film on a metal substrate, Nature 444, 350 (2006)
[6] F. Esch, T. Greber, S. Kennou, A. Siokou, S. Ladas, and R. Imbihl, The formation of a NO-NH3
coadsorption complex on a Pt(111) surface: a NEXAFS study, Catal. Lett. 38, 165 (1996).
Objectives for the three years
Study of atomic- and molecular-scale properties of model systems for gas-solid
heterogeneous catalysis.
Objectives for first year
Characterization and optimization of an LT-STM system to carry out vibrational
spectroscopy on single molecules by means of inelastic electron tunnelling (STM-IETS)
Design an analysis system for STM-IETS and action spectroscopy experimental data.
Practice on STM-IETS measurements, beginning form systems already described in
literature.
Characterization of the adsorption sites of CO2 on Ni(110), a model system for catalytic
hydrogenation of carbon dioxide to methanol.
 LT-STM characterization of NH3/Pt(111). This is both a test system to practice on action
spectroscopy measurements and a first step towards the study of the NH3 + NO
coadsorption complex on Pt(111).
Research project
STM measurements will be carried out by means of the commercial Omicron LT-STM available in
the SSR-STM laboratory at TASC.
The groundwork for STM-IETS measurements will start from the measurement of the noise
spectral density of the instrument and the transfer function of the tunnelling current amplifier. With
these information one will try to spot and remove the main sources of noise and to choose optimal
parameters for IETS spectra acquisition.
The data analysis system will be implemented in Igor Pro.
First test measurements will be carried out on carbon monoxide adsorbed over Cu(110).
CO2/Ni(110) system will be investigated by means of LT-STM microscopy.
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NH3/Pt(111) system will be studied with LT-STM microscopy, action spectroscopy and STMIETS.
The research of the following years will start from these model systems:
NH3 + NO complex over Pt(111), with STM-IETS; action spectroscopy and DFT
modeling;
Catalytic hydrogenation of CO2/Ni(110) to methanol with LT-STM microscopy and
action spectroscopy;
Adsorption and reactivity with hydrogen of carbon monoxide over Cu-Ni alloy, by means
of time-resolved core-level photoemission (XPS) with synchrotron light.
Educational activity
Heterogeneous catalisys, module of M.Sc. programme in Chemistry at Trieste University, taught by
prof. Paolo Fornsiero, 16 hours, March 2010.
Spring College on Computational Nanoscience at the Abdus Salam International Centre for
Theoretical Physics - Trieste, about 70 hours, may 2010.
Photoemission spectroscopies and spectromicroscopies, course of PhD programme in Physics at
Trieste University, taught by prof. Alessandro Baraldi, 25 hours, September-October 2010.
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Graduate Student: DAVIDE SAMMITO
Email: [email protected]
Laboratory: LILIT - TASC
Title of the thesis: Design, Fabrication and Characterization of Plasmonic Nanostructures
for Solar Cells Light Harvesting Enhancement
Supervisor: Filippo Romanato
Research Activity foreseen
State of the art and motivations
The research activity is called off in the area of plasmonics, a field of basic and applied research
which studies and exploits the particular properties of the electromagnetic fields generated at the
interface between metals and dielectrics. Light propagating in dielectrics cannot couple to surface
plasmons at flat metallic interface. Nevertheless, nanostructuring metal surfaces to get gratings or
realizing metal nanoparticles, it is possible to excite with ordinary light these electromagnetic
modes confined to surfaces and exploit the resulting near field enhancement.
During the three-year period of PhD fellowship I intend to study the plasmonic approach as a mean
to increase solar cells efficiency and decrease the cost per Watt of energy produced. This is one of
the targets of the European project ORION, to which most of the activities in my plan are related.
Plasmonic nanostructures can offer the possibility of reducing the physical thickness of the
photovoltaic absorber layers needed to convert a significant fraction on the incident photon flux in
free charge carriers. There are two main approaches proposed in literature. Metallic nanoparticles
can be used as subwavelength scattering elements to increase the optical path of the incoming light
inside the absorber, coupling energy to waves propagating horizontally in the device [1][2].
Metallic gratings integrated in the cell structure can couple sunlight into surface plasmon polariton
modes supported at the metal-absorber interface [3][4]. Conventional bulk Silicon solar cells could
benefit from plasmonic nanostructures too: the absorption profile of photons with wavelengths close
to the bandgap, which normally are scarcely absorbed, could be reshaped and enhanced in regions
of the cell with high collection probability for the photogenerated carriers.
Bibliography

Pillai S., Catchpole K. R., Trupke T., Green M.A. 2007, Journal of Applied Physics,
Vol. 101, p. 093105.

F. J. Beck, S. Mokkapati, A. Polman, K. R. Catchpole 2009, Proceedings European
Photovoltaic Solar Energy Conference, 232 – 235.

Pala R., White J., Barnard E., Liu J., Brongersma M. 2009, Advanced Materials, Vol.
21, pp. 1-6.

S. Collin, F. Pardo, N. Bardou, S. Averin, J.-L. Pelouard 2009, Proceedings
European Photovoltaic Solar Energy Conference, 239 – 240.
Objectives for the three years
Numerical modelling of the electro-optical behaviour of bulk and thin film Silicon solar cells,
both single- and multi-junction type, integrating plasmonic nanostructures.
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Designing of one or more layouts of bulk or thin film solar cells and their relative process flow
in order to obtain enhancement of the efficiency by plasmonic effects.
Realization and characterization of samples for some of the designed layouts.
Objectives for the first year
Implementation of a numerical model for the electro-optical simulation of crystalline Silicon
bulk solar cells integrating plasmonic crystals.
Implementation of a numerical model for the electro-optical simulation of amorphous Silicon
thin film solar cells integrating plasmonic gratings.
Development of lithographic processes and fabrication of prototypes of plasmonic gratings to
be integrated in solar cells.
Development of a goniometric stage for the positioning of samples for X-ray lithography.
Upgrading and testing of the setup for electro-optical characterization of solar cells at TASC
laboratory.
Electro-optical characterization of bulk solar cells integrating plasmonic gratings.
Research project
The activities to be carried out can be divided into three work packages: 1) design and
modellization; 2) nanofabrication; 3) characterization.
The designing step includes the definition of possible configurations to integrate plasmonic
nanostructures in solar cells and the study of the properties of the electromagnetic field generated
by the interaction of sunlight with plasmonic gratings. One of the objectives is to realize an electrooptical model of solar cells to evaluate the effect of the integrated nanostructures in cell
performance and to optimize the geometrical and material parameters of the nanostructures. Initially
the behaviour of conventional solar cells, single junction bulk crystalline Silicon cells, will be
simulated. Subsequently thin film cells will be analyzed, for which a higher enhancement of the
performance is expected due to the optical trapping mechanism generated by surface plasmons.
Then solar cells with more complex structures, such as multiple junctions cells, will be studied.
The nanofabrication step has an essential role in the project. The requirements for the lithographic
processes are the capability of realizing structures with feature size of the order of few hundred
nanometers, to obtain gratings which interact with visible and near infrared light, and the
compatibility with industrial mass production processes. It will be necessary to develop additive
(thin film deposition) and subtractive (dry etching) processes to define the structure of the cells and
of the plasmonic gratings. X-Ray lithography will be used to realize test structures and masters for
nanoimprinting lithography. Initially the gratings will be integrated in bulk crystalline Silicon solar
cells provided by partners of the ORION project. Then applications on suitable thin film cells will
be analyzed.
The solar cells with the integrated plasmonic nanostructures will be object of morphological (SEM)
and electro-optical characterization (e.g. I-V curve, quantum efficiency measurements, reflectance).
In case new materials will be tested as absorbers for solar cells, structural and electronic properties,
such as minority carrier lifetime and charge transport properties, could be investigated by
synchrotron radiation experiments.
In the next page the Gantt diagram for first year activities is reported.
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Educational activity
1
1.1
December
November
October
August
July
June
May
April
March
Activity
February
WP
January
Month
September
With the aim to improve the skills in modellization, I intend to use the hours of educational activity
broadening my studies in the physics of inorganic semiconductor opto-electronic devices.
Design and Modelling
Optical
model
of
plasmonic gratings
1.2
Electro-optical model
single junction c-Si cell
1.3
Electro-optical model
single junction a-Si cell
2
Nanofabrication
2.1
Development of a RIE
process for pattern
transfer into substrate
2.2
Fabrication of test
plasmonic gratings
2.3
Development
of
processes
for
the
integration of gratings
in bulk cells
2.4
Goniometric stage per
X-ray lithography
3
Characterization
3.1
Upgrade of the setup
for cell electro-optical
characterization
3.2
Electro-optical
characterization of bulk
cells
3.3
Optical characterization
of test gratings
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5.Pubblicazioni dei dottorandi.
Venogono di seguito riportate le pubblicazioni dei dottorandi desunte dalla schede riportate al punto
precedente:
Pubblicazioni su riviste scientifiche (pubblicate o accettate per la pubblicazione)
1. Barinov, A., Malcioǧlu, O.B., Fabris, S., Sun, T., Gregoratti, L., Dalmiglio, M.,
Kiskinova, M. Initial stages of oxidation on graphitic surfaces: Photoemission study and
density functional theory calculations, 2009, Journal of Physical Chemistry C 113 (21),
pp. 9009-9013
2. Bozzini, B., Dalmiglio, M., De Gaudenzi, G.P., D'Urzo, L., Gregoratti, L. An
investigation of the corrosion of WC-Co cermets in CN--containing aqueous solutions.
Part II: Synchrotron-based high lateral-resolution XPS study, 2009, Corrosion Science 51
(8), pp. 1675-1678
3. M. Dalmiglio, M. Amati, L. Gregoratti, T. O. Menteş, M. A. Niño, L. Felisari and M.
Kiskinova, Catalytic activity of PtRh particles, submitted to Journal of Physical
Chemistry, 2010
4. P. Lacovig, M. Pozzo, D. Alfè, P. Vilmercati, A. Baraldi, S. Lizzit, Growth of DomeShaped Carbon Nanoislands on Ir(111): The Intermediate between Carbidic Clusters and
Quasi-Free-Standing Graphene, Phys. Rev. Lett. 103, 166101 (2009)
5. E. Golfetto, A. Baraldi, M. Pozzo, D. Alfè, A. Sala, P. Lacovig, E. Vesselli, S. Lizzit, G.
Comelli, R. Rosei, Determining the chemical reactivity trends of Pd/Ru(0001)
pseudomorphic overlayers: core level shift measurements and DFT calculations, J. Phys.
Chem. C, in press DOI: 10.1021/jp908568v, 2010
6. T. Montini, A. Speghini, L. De Rogatis, B. Lorenzut, M. Bettinelli, M. Graziani and P.
Fornasiero, “The identification of the structural phases of CexZr1-xO2 by Eu(III)
luminescence studies.”, Journal of the American Chemical Society 131 (2009), 1315513160
7. S. Specchia, L. D. Vella, B. Lorenzut, T. Montini, V. Specchia and P. Fornasiero, “Effect
of the catalyst load on syngas production in SCT-CPO reactors.”, Industrial and
Engineering Chemistry Research (2009), accepted for publication,
doi:10.1021/ie900695j.
8. L. De Rogatis, M. Cargnello, V. Gombac, B. Lorenzut, T. Montini and P. Fornasiero,
“Embedded phases: a way to active and stable catalysts”, ChemSusChem (2009),
accepted for publication
9. E. Conca, T. Negri, A. Gronchi, E. Fumagalli, E. Tamborini, G.M. Pavan, M. Fermeglia,
M.A. Pierotti, S. Pricl, S. Pilotti. Activate and resist: L576P-KIT in GIST. Molecular
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Cancer Therapeutics. Mol Cancer Ther 1535-7163.MCT-09-0662. Mol Cancer Ther
8(9):2491-5, 2009.
10. S.E. Woodman, J.C. Trent, K. Stemke-Hale, A.J. Lazar, S. Pricl, G.M. Pavan, M.
Fermeglia, Y.N. Gopal, D. Yang, D.A. Podoloff, D. Ivan, K.B. Kim, N. Papadopoulos, P.
Hwu, G.B. Mills and M.A. Davies. Activity of dasatinib against L576P KIT mutant
melanoma: Molecular, cellular, and clinical correlates. Mol Cancer Ther 8(8):2079-85,
2009.
11. T. Negri, G.M. Pavan, S. Pricl, S. Lagonigro, F. Miselli, A. Greco, M. Fermeglia, M.A.
Pierotti, S. Pilotti, E. Tamborini, T670X KIT mutations in GISTs: making sense of
missense. J Natl Cancer Inst 2009;101:1–11.
12. S.E. Woodman, J.C. Trent, K. Stemke-Hale, A. Lazar, S. Pricl, G.M. Pavan, N.
Papadopoulos, P. Hwu, G.B. Mills, M.A. Davies. Selective Activity of Dasatinib for the
Most Common KIT Mutation in Melanoma (L576P). Journal of Clinical Oncology, 2009
ASCO Annual Meeting Proceedings (Post-Meeting Edition). Vol 27, No 15S (May 20
Supplement), 2009: 9019.
13. Toth R, Voorn DJ, Handgraaf JW, Fraaije JGEM, Fermeglia M, Pricl S, Posocco P.
Multiscale computer simulation studies of water-based montmorillonite/poly(ethylene
oxide) nanocomposites. Macromolecules, 2009, on web (ma200901584w).
14. Scocchi G, Posocco P, Handgraaf JW, Fraaije JGEM, Fermeglia M, Pricl S. A complete
multiscale modelling approach for polymer-clay nanocomposites. Chemistry - A
European Journal, 15, 7586-7592, 2009.
15. Posocco P, Lisal M, Posel Z, Fermeglia M, Pricl S. A multiscale molecular simulation
approach to the prediction of the morphology of self-assembled nanoparticles in diblock
copolymers. Journal of Physical Chemistry B, submitted.
16. Posocco P, Fermeglia M, Pricl S. A multiscale toolbox for morphology prediction of
diblock copolymers for drug delivery. Biomacromolecules, submitted.
17. Pricl S, Posocco P, Scocchi G, Fermeglia M. “Polymer-clay nanocomposites”, in
Handbook of Nanophysics, Klaus Sattler Editor, Taylor&Francis Publisher, 2009.
18. Polyol Synthesis of Silver Nanoparticles: Mechanism of Reduction by Alditol Bearing
Polysaccharides (Ivan Donati, Andrea Travan, Chiara Pelillo, Anna Coslovi, Alois
Bonifacio,Valter Sergo, Sergio Paoletti). Biomacromolecules, 2009, 10 (2), 210-213
19. Non-Cytotoxic Silver Nanoparticle – Polysaccharide Nanocomposites with Antimicrobial
Activity (Andrea Travan, Chiara Pelillo, Ivan Donati, Eleonora Marsich, Monica
Benincasa, Tommaso Scarpa, Sabrina Semeraro, Gianluca Turco, Renato Gennaro,
Sergio Paoletti). Biomacromolecules 2009, 10, 1429–1435
20. Surface Modification and Polysaccharide Deposition on BISGMA/TEGDMA
Thermosets (Andrea Travan, Ivan Donati, Eleonora Marsich, Francesca Bellomo,
Satish Achanta, Sabrina Semeraro, Tommaso Scarpa, Mila Toppazzini, Vittorio
Spreafico, and Sergio Paoletti). Biomacromolecules 2010. (DOI: 10.1021/bm9011419)
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21. Silver-Polysaccharide Nanocomposite Antimicrobial Coatings for Methacrylic
Thermosets (Andrea Travan, Eleonora Marsich, Ivan Donati, Monica Benincasa, Marta
Giazzon, Laura Felisari, Sergio Paoletti. Submitted to European Cells and Materials
Journal (2010)
22. Characterization of silver nanoparticles for biomedical applications by means of
quantitative analysis of TEM micrographs (Francesco Brun, Andrea Travan, Agostino
Accardo, Sergio Paoletti). Biomedical Sciences Instrumentation 2010 (In press).
23. Silver nanocomposites and their biomedical applications (Andrea Travan, Eleonora
Marsich, Ivan Donati, Sergio Paoletti). Chapter of the book entitled “Nanocomposites for
the Life Sciences”. In press, Wiley-VCH (2010)
24. Journal of Materials Science, “Raman investigation of the ageing of Ni-BaTiO3
multilayer ceramic capacitors”, A.Umeri, T.A. Kuku, N.Scuor and V.Sergo, Volume 43,
Issue 3, P.922-926,2008-02-01, DOI - 10.1007/s10853-007-2215-4, 2010
25. L. Rigon, F. Arfelli, A. Astolfo, A. Bergamaschi, D. Dreossi, R. Longo, R.H. Menk, B.
Schmitt, E. Vallazza, E. Castelli, “A single-photon counting “edge-on” silicon detector
for synchrotron radiation mammography”, Nucl. Instr. And Meth. A, 608, S62-S65, 2009
26. Alginate/Hydroxyapatite biocomposite for bone ingrowth: a trabecular structure with
high and isotropic connectivity.Turco G, Marsich E, Bellomo F, Semeraro S, Donati I,
Brun F, Grandolfo M, Accardo A, Paoletti S. Biomacromolecules. 2009 Jun 8;
10(6):1575-83
27. Surface modification and polysaccharide deposition on BISGMA/TEGDMA based
resins. Donati I., Travan A., Marsich E., Bellomo F., Achanta S., Semeraro S., Scarpa T.,
Toppazzini M., Spreafico V., Paoletti S. Submitted to Biomacromolecules, 2010
28. Giovanni Birarda, Gianluca Grenci, Luca Businaro, Benedetta Marmiroli, Sabrina Pacor,
Lisa Vaccari - “Fabrication of a microfluidic platform for investigating dynamic
biochemical processes in living samples by FTIR microspectroscopy – Microelectronic
Engineering (in press), 2010
29. Giovanni Birarda, Gianluca Grenci, Luca Businaro, Benedetta Marmiroli, Sabrina Pacor,
Federica, Piccirilli, Lisa Vaccari - Infrared microspectroscopy of biochemical response
of living cells in microfabricated devices” – Vibrational Spectroscopy (submitted evaluated) 2009
30. D‟Amelio N, Fontanive L, Uggeri F, Suggi-Liverani F, Navarini L. NMR reinvestigation
of the caffeine-chlorogenate complex in acqueous solution and in coffee brews. Food
Biophys. 2009, 4, 321-330.
31. Cadenaro M, Marchesi G, Antoniolli F, Davidson CL, De Stefano Dorigo E, Breschi L.
“Flowability of composites is no guarantee for contraction stress reduction.” Dental
Materials 2009;25(5):649-54.
32. Mazzoni A, Marchesi G, Cadenaro M, Mazzotti G, Di Lenarda R, Ferrari M, Breschi M.
“Push-out stress for fiber posts luted using different adhesive strategies.” European
Journal Oral Science 2009;117(4):447-453.
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33. Cadenaro M, Pashley DH, Marchesi G, Carrihlo M, Antoniolli F, Mazzoni A, Tay FR, Di
Lenarda R, Breschi L. “Influence of chlorexidine on the degree of conversion and Emodulus of experimental adhesive blends”. Dental Materials 2009;25(5):1269-74.
34. Marchesi G, Breschi L, Antoniolli F, De Stefano Dorigo E, Ferracane J, Cadenaro M.
“Contraction stress of composite materials with different polymerization reactions
assayed by low-and high-compliance systems. Dental Materials submitted, 2010.
35. Breschi L, Cadenaro M, Mazzoni A, Navarra CO, Marchesi G, Di Lenarda R, De Stefano
Dorigo E. “La durata del legame adesivo:la nuova sfida dell‟ odontoiatria resteurativa.”
Dentista Moderno 2009;4:41-54.
36. “Dependence of the fractional anisotropy in cervical spine from the number of diffusion
gradients, repeated acquisition and voxel size”, Santarelli, X., Garbin, G., Ukmar, M.,
Longo, R.; Magnetic Resonance Imaging (in press), 2010
37. 1) V. Gombac, L. Sordelli, T. Montini, J. J. Delgado, A. Adamski, G. Adami, M.
Cargnello, S. Bernal and P. Fornasiero, “CuOx-TiO2 Photocatalysts for H2 Production
from Ethanol and Glycerol Solutions.”, The Journal of Physical Chemistry A, in press,
doi:10.1021/jp907242q, 2010.
38. 2) L. De Rogatis, M. Cargnello, V. Gombac, B. Lorenzut, T. Montini and P. Fornasiero,
“Embedded phases: a way to active and stable catalysts.”, ChemSusChem, in press, 2010.
39. 3) M. Cargnello, T. Montini, S. Polizzi, N. L. Wieder, R. J. Gorte, M. Graziani and P.
Fornasiero, “Novel embedded Pd@CeO2 catalysts: a way to active and stable catalysts.”,
Dalton Transactions, in press, 2010.
40. 1) M. Cargnello, N. L. Wieder, T. Montini, R. J. Gorte and P. Fornasiero, “Synthesis of
dispersible Pd@CeO2 core-shell nanostructures by self-assembly”, Journal of the
American Chemical Society, submitted.
41. “The evolutionary conserved gene C16orf35 encodes a nucleo-cytoplasmic protein that
interacts with p73.” Lunardi A, Chiacchiera F, D'Este E, Carotti M, Dal Ferro M, Di
Minin G, Del Sal G, Collavin L. Biochem Biophys Res Commun. 2009 Oct
16;388(2):428-33.
42. Corona G, Elia C, Casetta B, Diana C, Rosalen S, Bari M, Toffoli G. A liquid
chromatography-tandem mass spectrometry method for the simultaneous determination
of exemestane and its metabolite 17-dihydroexemestane in human plasma. J Mass
Spectrom. 2009 Jun.
43. Corona G, De Lorenzo E, Elia C, Simula MP, Avellini C, Baccarani U, Lupo F, Tiribelli
C, Colombatti A, Toffoli G. Differential protomi analysis of hepatocellular carcinoma.
Internation Journal of Oncology 2009 (accepted for publication)
44. Migliorini E., Ban J., Di Foggia V., Ruaro ME., Torre V., Pozzato A., Grenci G., Tormen
M, Lazzarino M. Effect of nanopatterned substrates on growth cones activity. Eur
Biophys .J. 2009 Oct, (suppl 1) S89
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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45. Kondra S, Laishram J., J. Ban J.,Migliorini E., Di Foggia V., Lazzarino M., Torre V.,
Ruaro M.E. Integration of Confocal and Atomic Force Microscopy. Journal of
Neuroscience methods. 2009 Feb 15;177(1):94-107. Epub 2008 Oct 18.
46. Laishram J., Kondra S., Avossa D., Migliorini E., Lazzarino M., Torre V. A
morphological analysis of growth cones of DRG neurons combining Atomic Force and
Confocal Microscopy. Journal of Structural Biology. 2009 Sept.
47. R.Muscia, G.Sciuto, F. Zuddas – On the usage of PCM elements within asphalt concrete
to avoid ice formation on roadways. International Journal of Solar Energy, submitted
2010.
Pubblicazioni/abstracts in conferenze/congressi (nazionali o internazionali)
48. POSTER - P. Lacovig, M. Pozzo, D. Alfè, P. Vilmercati, A. Baraldi, and S. Lizzit,
Graphene Growth on Ir(111): A Fast XPS Study - CINF-DTU Summer School on
“Reactivity of nanoparticles for more efficient and sustainable energy production”,
Sandbjerg Gods (Denmark), 2009
49. POSTER – P. Lacovig, A. Baraldi, S. Lizzit, Chemical reactivity of nanoclusters
deposited on solid surfaces: new time-domain and enhanced surface-sensitivity of
photoelectron spectroscopy - Joint Workshop of SFB762 & IMPRS on “Magnetic
properties of oxidic and multiferroic systems”, Wittenberg (Germany), 2009
50. Posocco P, Fermeglia M, Pricl S. Multiscale molecular modeling of di/triblock
copolymers for drug delivery. FOMMS, Blaine, USA, (2009).
51. Fermeglia M, Posocco P, Pricl S, Fraaije JGEM, Handgraaf JW, Scocchi G, Danani A. A
complete multiscale modeling approach for nanocomposites. Eurofillers, Alessandria,
Italy, (2009).
52. Fermeglia M, Posocco P, Pricl S, Fraaije JGEM, Handgraaf JW, Deglmann P, KurkalSiebert V, Weiss H. On the DPD Parameter Estimation From Atomistic/Quantum
Mechanics Information. AIChE Annual meeting, Nashville, USA, (2009).
53. Fermeglia M, Scocchi G, Pricl S, Handgraaf JW, Fraaije JGEM, Posocco P. A Complete
Multiscale Modeling Approach for Polymer-Clay Nanocomposites. AIChE Annual
meeting, Nashville, USA, (2009).
54. Posocco P, Fermeglia M, Pricl S. The Long and Winding Road. Multiscale Molecular
Modeling Approach to the Self-Assembly of Di/Triblock Copolymers for Drug Delivery
in Aqueous Solution. AIChE Annual meeting, Nashville, USA, (2009).
55. Posocco P. Modellistica molecolare multiscala per la progettazione di materiali
nanostrutturati. I giovani e la chimica in FVG, Trieste, (2009).
56. G.M. Pavan, P. Posocco, M. Fermeglia, G. Scocchi, A. Malek, M.Maly, A. Danani, C.
Catapano, S. Pricl. Nanocarriers in delivery of small interfering RNA theurapetics. 9th
International Conference on Chemical and Process Engineering. Rome, 10-13 May 2009.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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57. POSTER - “Nanostructured Zirconia in dental applications: new problems for an old
material” – A.Umeri, A. Bonifacio and V. Sergo – National Nanomedicine Conference –
28,29 November 2008 - Genova
58. Spatio-Temporal Radiation Biology: Transdiscipinary Advances for Biomedical
Applications – Booklet of abstracts (Saint Feliu de Guixols, Spain, 16-21 May 2009), A.
Astolfo, R.-H. Menk, F. Arfelli, E. Schultke, C. Hall, L. Rigon, B.H.J. Juurlink, “Long
Term Cell Tracking in Small Animals Phase Using Phase Contrast Based Micro CT and
Synchrotron Radiation”, P.53.
59. “POROUS NANO-HYDROXYAPATITE/ALGINATE SCAFFOLDS FOR BONE
REGENERATION” Eleonora Marsich, Sabrina Semeraro, Gianluca Turco, Francesca
Bellomo, Ivan Donati and Sergio Paoletti (7-9 novembre 2008 Bone-tec Hannover; Joint
Workshop of SFB762 & IMPRS 10th/11thSeptember 2009 in the Leucorea at
Wittenberg)
60. “SPERIMENTAZIONE IN VIVO DI SCAFFOLD DI ALGINATO E
IDROSSIAPATITE PER RIGENERAZIONE OSSEA” F. Bellomo, M. Biasotto,
G.Turco, R. Di Lenarda, S. Paoletti (22° riunione nazionale “A. Castellani” dei
dottorandi di ricerca in discipline biochimiche Brallo di Pregola (PV) 9 – 12 Giugno
2009; Collegio dei docenti di odontoiatria Roma 22-24 aprile 2009)
61. The Proceedings of Conference on Miniaturized Systems for Chemistry and Life
Sciences (MicroTAS 2009), Giovanni Birarda,Gianluca Grenci, Luca Businaro,
Benedetta Marmiroli, Giuseppe Bellisola and Lisa Vaccari, Single Living Cell
Biochemical Rearrangements Investigated By Infrared Microspectroscopy In
Microfluidic Devices - 978-0-9798064-2-1/μTAS2009/$20ⓒ2009CBMS pp 338-341.
62. Marchesi G, Cadenaro M, Antoniolli F, Di Lenarda R, Pashley DH, Breschi L.
“Influenza della clorexidina sul grado di conversione e sul modulo elastico in adesivi
sperimentali.” Acts of XIII national meeting SIDOC-Rome 19-21/2/2009.
63. Marchesi G, Breschi L, Antoniolli F, De Stefano Dorigo E, Cadenaro M. “Stress da
polimerizzazione di una resina a base siloranica.”Acts of national meeting Docenti di
Odontoiatria e di chirurgia Maxillofacciale, Roma 22-24/4/2009.
64. Marchesi G, Cadenaro M, Breschi L, Antoniolli F, Tay FR, Di Lenarda R, Pashley DH.
“Influence of chlorexidine on the E-modulus of experimental resins.” J Dent Res 88 (spec
Iss A). Annual meeting of The International Association for Dental Research. Miami FL
USA.
65. Marchesi G, Cadenaro M, Di Lenarda R, Breschi. Bond strenght of Post Luted with
different Self-adhesive Resin Cements. Annual meeting of the Accademy Dental
Materials. Portland Oregon USA 29-31/10/2009
66. Marchesi G. Analysis of aging and stability of bonded interface in dentistry. Poster 2°
Workshop of the SFB 762-Wittenberg, Germany 10-11/09/2009.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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67. ORAL:“Local BDNF delivery by means of laser tweezers to stimulate hippocampal
neurons signalling” Elisa D‟Este, Gabriele Baj, Paolo Beuzer, Enrico Ferrari, Federica
Tavano, Enrico Tongiorgi, Dan Cojoc; ICTP-Introduction to Optofluidics
68. ORAL: “High spatial resolution BDNF delivery by means of laser tweezers to stimulate
hippocampal neurons signalling”; Elisa D‟Este, Gabriele Baj, Paolo Beuzer, Enrico
Ferrari, Federica Tavano, Enrico Tongiorgi, Dan Cojoc; EBSA 2009
69. ORAL: “High Resolution Drug Delivery by Optical Tweezers Microscopy” Elisa
D‟Este, Gabriele Baj, Paolo Beuzer, Enrico Ferrari, Federica Tavano, Enrico Tongiorgi,
Dan Cojoc; Super and High Resolution Imaging 2009
70. POSTER: “Optical Tweezers as means to stimulate hippocampal neurons with BDNF
coated beads”; Elisa D‟Este, Gabriele Baj, Paolo Beuzer, Enrico Ferrari, Federica
Tavano, Enrico Tongiorgi, Dan Cojoc; Nanobioviews-2nd international symposium on
Optical Tweezers in life sciences; Award best poster Optical Tweezers
71. Corona G, Elia C, Casetta B and Toffoli G. Liquid Chromatography/Tandem Mass
Spectrometry Method To Rapid Asses the Irinotecan Metabolic Phenotype. Mass Spec
Europe 5-6 November 2009, Barcelona, Spain
72. G.Sciuto, R.Muscia – Multiobjective optimization of intracoronary stents by genetic
algorithm. 13th international research/expert conference, Trends in the development of
machinery and associated technology, TMT 2009, Hammameth, Tunisia.
73. R.Muscia, G.Sciuto – A method for performing quality comparisons of Rzeppa joints.
13th international research/expert conference, Trends in the development of machinery
and associated technology, TMT 2009, Hammameth, Tunisia.
74. 1. Poster: “High spatial resolution BDNF delivery by means of laser tweezers to
stimulate hippocampal neurons signalling”; Elisa D‟Este, Gabriele Baj, Paolo Beuzer,
Enrico Ferrari, Federica Tavano, Enrico Tongiorgi, Dan Cojoc; EBSA 2009
75. Poster: “High Resolution Drug Delivery by Optical Tweezers Microscopy” Elisa D‟Este,
Gabriele Baj, Paolo Beuzer, Enrico Ferrari, Federica Tavano, Enrico Tongiorgi, Dan
Cojoc; Super and High Resolution Imaging 2009
76. Poster: “Optical Tweezers as means to stimulate hippocampal neurons with BDNF
coated beads”; Elisa D‟Este, Gabriele Baj, Paolo Beuzer, Enrico Ferrari, Federica
Tavano, Enrico Tongiorgi, Dan Cojoc; Nanobioviews-2nd international symposium on
Optical Tweezers in life sciences
77. “FARMACOGENETICA DELLA CARDIOTOSSICITÀ DA ANTRACICLINE”,
Chiara Zanusso and Giuseppe Toffoli, La gestione delle problematiche” at Napoli on
March 25-26, 2009.
78. “NANOTECHNOLOGIES AND ONCOLOGY: PHARMACOKINETICS AND
PHARMACOGENOMICS TO OPTIMIZE THE ANTITUMOR THERAPIES”, Chiara
Zanusso, Erika Cecchin, Paola Biason, Elena De Mattia, Franca Sartor and Giuseppe
Toffoli, 2nd Phd workshop 10th/11 September 2009, Wittenberg.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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Brevetti
79. Italian Patent FI2007A0000179 “Catalizzatori per la produzione di gas di sintesi da
reforming di idrocarburi o alcoli comprendenti un supporto in ZnO e loro uso” ;
Inventors: Paolo BERT, Stefano CATANORCHI, Paolo FORNASIERO, Mauro
GRAZIANI, Barbara LORENZUT, Hamish MILLER, Tiziano MONTINI, Marina
RAGNOLI, Adriana SCAFFIDI, Alessandro TAMPUCCI.
80. Materiali nanocompositi basati su nanoparticelle metalliche stabilizzate con polisaccaridi
a struttura ramificata (Andrea Travan, Ivan Donati, Eleonora Marsich, Sergio Paoletti,
Brevetto 8853 PTIT)
81. Materiali nanocompositi formati da una matrice polisaccaridica e nanoparticelle
metalliche, loro preparazione ed uso (Andrea Travan, Ivan Donati, Eleonora Marsich,
Sergio Paoletti, Brevetto 8981 PTIT)
82. Nanocomposite materials based on metallic nanoparticles stabilized with branched
polysaccharides (Andrea Travan, Ivan Donati, Elonora Marsich, Sergio Paoletti). PCT
Patent extension (2009)
83. Three-dimensional nanocomposite materials consisting of a polysaccharidic matrix and
metallic nanoparticles, preparation and use thereof (Andrea Travan, Ivan Donati, Elonora
Marsich, Sergio Paoletti). PCT Patent extension (2009)
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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6. Giudizi della commissione d’esame finale 2009
La commissione composta dai Professori Dragan Mihailovic, Istituto Josef Stefan, Ljubljana (SLO),
Kostas Karatasos, Università di Thessaloniki, Dipartimento di Ingegneria Chimica (G) e Tullio
Giraldi, Università di Trieste (membro interno), hanno sentito i candidati e stilato i seguenti giudizi:
Matteo Maria DALMIGLIO
Il candidato ha presentato il suo lavoro di tesi che ha ha avuto come obiettivo quello di studiare la
reattività e le proprietà di ossidazione di cluster di catalizzatori supportati da ossidi o da nanotubi di
carbonio, mediante tecniche diverse, tra cui la spettro-microscopia a scansione in fotoemissione
(SPEM).
La commissione ha apprezzato il livello scientifico del lavoro svolto ed ha individuato una
potenzialità per applicazioni.
Il candidato ha dimostrato di possedere una notevole conoscenza della materia in studio,
affrontando la discussione con competenza nelle tecniche proprie del lavoro di tesi. Il candidato ha
risposto compiutamente ed in modo chiaro alle domande dei Commissari.
Approvata la relazione, la Commissione propone che al candidato VENGA conferito il titolo di
Dottore di Ricerca.
Paolo LACOVIG
Il candidato ha illustrato il suo lavoro di tesi che è consistita sia dello sviluppo di strumentazione
innovativa per la misura di spettri di fotoemissione e per lo studio della struttura elettronica, della
morfologia e le proprietà chimiche di sistemi a bassa dimensionalità. La risoluzione temporale
raggiunta dal nuovo analizzatore ha consentito poi di studiare dei processi dinamici fino ad ora
inaccessibili, in particolare la crescita ad alta temperatura del grafene su diversi metalli di
transizione e la reattività chimica di nano-aggregati atomici.
La commissione ha apprezzato il lavoro innovativo e di frontiera affronatato dal candidato che ha
dimostrato di possedere una ottima conoscenza della materia in studio, presentando con competenza
e con completezza il suo lavoro di tesi, rispondendo puntualmente e compiutatamente alle domande
della Commissione.
Approvata la relazione, la Commissione propone che alla candidata VENGA conferito il titolo di
Dottore di Ricerca.
Barbara LORENZUT
La candidata ha illustrato il suo lavoro di tesi che ha riguardato lo sviluppo e la caratterizzazione di
sistemi catalitici per la produzione dell‟idrogeno. In particolare sono stati studiati catalizzatori per il
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
127
reforming di metanolo ed etanolo a base di metalli di transizione e per la produzione di idrogeno
attraverso la reazione di cracking dell‟ammoniaca e lo steam reforming del glicerolo su catalizzatori
a base di platino.
La commissione ha apprezzato il livello scientifico del lavoro svolto e la applicabilità diretta su
temi industriali.
La candidata ha dimostrato di possedere una notevole conoscenza della materia in studio,
affrontando la discussione con competenza nelle tecniche proprie del lavoro di tesi e rispondendo
compiutamente ed in modo chiaro alle domande dei Commissari.
Approvata la relazione, la Commissione propone che alla candidata VENGA conferito il titolo di
Dottore di Ricerca.
Giovanni Maria PAVAN
Il candidato ha presentato il suo lavoro di tesi che ha riguardato la terapia mirata in oncologia, con
particolare riguardo a sistemi farmaco/oncogene caratteristici di patologie come la leucemia
mieloide cronica e il tumore gastrointestinale stromale, attraverso l‟impiego di metodi di
simulazione molecolare. Lo studio ha evidenziando come inibitori interagiscono con i siti di
binding. Confronti con dati sperimentali hanno evidenziato un‟ottima corrispondenza tra i dati
ottenuti in silico e le evidenze cliniche, dimostrando l‟affidabilità e le potenzialità della simulazione
come strumento d‟indagine biomedica.
La commissione ha apprezzato il livello scientifico del lavoro svolto e la applicabilità diretta su
problematiche di interpretazione di dati preclinici e clinici.
Il candidato ha dimostrato di possedere una notevole conoscenza della materia in studio,
affrontando la discussione con competenza nelle tecniche proprie del lavoro di tesi e rispondendo
compiutamente ed in modo adeguato alle domande dei Commissari.
Approvata la relazione, la Commissione propone che al candidato VENGA conferito il titolo di
Dottore di Ricerca.
Paola POSOCCO
La candidata ha illustrato il suo lavoro di tesi che ha avuto come argomento principale lo sviluppo
di procedure multiscala per la simulazione di materiali nanostrutturati di inteesse industriale.
compositi polimero – silicati. I sistemi analizzati appartengono a settori diversi tra loro, si va dai
materiali nanocompositi lamellari, alle resine epossidiche, ai copolimeri a blocchi, ai gel per uso
biomedico. In tutti i casi è stato riscontrato un buon accordo tra le evidenze sperimetnali ed i dati
simualti.
La commissione ha apprezzato la completezza e il livello scientifico elevato del lavoro svolto. La
candidata ha dimostrato di possedere una ottima conoscenza della materia in studio, presentando
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
128
con competenza e con completezza il suo lavoro di tesi, rispondendo puntualmente alle domande
della Commissione.
Approvata la relazione, la Commissione propone che alla candidata VENGA conferito il titolo di
Dottore di Ricerca.
Andrea TRAVAN
Il candidato ha presentato il suo lavoro di tesi relativo allo sviluppo di sistemi nanocompositi per
applicazioni biomediche con scopo ultimo di realizzare un rivestimento per protesi ortopediche
dotato di proprietà antimicrobiche. Come agenti antimicrobici sono stati studiati sistemi
nanocompositi a base di polisaccaridi di origine naturale che stabilizzano nanoparticelle metalliche
in soluzione colloidale ed in matrici solide tridimensionali (gel). Nanocompositi sono stati
sviluppati e caratterizzati nel lavoro di tesi.
La commissione ha apprezzato l‟interdisciplinarietà della ricerca che ha utilizzato molte delle
risorse scientifiche della scuola. Il candidato ha dimostrato di possedere una ottima conoscenza
della materia in studio, presentando con molta competenza e con completezza il suo lavoro di tesi,
rispondendo puntualmente alle domande della Commissione
Approvata la relazione, la Commissione propone che al candidato VENGA conferito il titolo di
Dottore di Ricerca.
Andrea UMERI
Il candidato ha presentato il suo lavoro di tesi che ha avuto come argomento principale l‟analisi di
compositi Allumina Zirconia in relazione al fenomeno di aging della Zirconia, alla luce del
rinnovato interesse di questo materiale in campo dentistico. Campioni che hanno subito
invecchiamento naturale ed artificiale sono stati anlizzati a diverse concentrazioni tramite
spettroscopia Raman and AFM.
La commissione ha apprezzato il livello scientifico del lavoro svolto e la applicabilità diretta su
temi di interesse pratico.
Il candidato ha dimostrato di possedere una notevole conoscenza della materia in studio,
affrontando la discussione con competenza nelle tecniche proprie del lavoro di tesi e rispondendo
compiutamente ed in modo chiaro alle domande dei Commissari.
Approvata la relazione, la Commissione propone che al candidato VENGA conferito il titolo di
Dottore di Ricerca.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
129
7.Elenco dei componenti del collegio docenti
Componente
Ente
Dipartimento
SSD
BARALDI
Alessandro
BONIN Serena
UNITS
Department of Physics - DF
UNITS
BRESCHI
Lorenzo
CADENARO
Milena
CASALIS
Loredana
CESARO Attilio
UNITS
Department of Anesthesia, Surgery, MED/34 RU
Pathological Anatomy, Dermatology,
Pathology and Molecular Sciences
Clinical operative Unit of Odontoiatry MED/28 PA
and Stomatologic - UCOOS
Clinical operative Unit of Odontoiatry MED/28 PA
and Stomatologic - UCOOS
Not assigned
RIC
COJOC Dan
INFM-TASC
UNITS
Elettra
Synchrotron Spa
UNITS
Department of Life Sciences - DLS
COMELLI
UNITS
Giovanni
CONTIN Alfredo UNITS
DA ROS Tatiana UNITS
DANANI Andrea University
School Italian
Swiss
DE STEFANO
UNITS
DORIGO Elettra
DE VANNA
UNITS
Maurizio
DE VITA
Alessandro
DI FABRIZIO
Enzo
DI LENARDA
Roberto
FASSINA
Ambrogio
FERMEGLIA
Maurizio
FIRRAO
Giuseppe
FLORIAN Fiorella
UNITS
FIS/03
Qualifica
RU
CHIM/04 PO
Not assigned
Department of Physics - DF
FIS/03
PO
Department of Electronics,
ING/IND PA
Electrotecnics and Informatics - DEEI
Department of Pharmaceutical
CHIM/08 RU
Sciences - DSF
Not assigned
RIC
Clinical operative Unit of Odontoiatry
and Stomatologic - UCOOS
Department of Medical,
Technological and Traslational
Sciences - DMTTS
Department of Materials and Natural
Resources - DMNR
Not assigned
MED/28 PO
MED/25 PA
PO
Clinical operative Unit of Odontoiatry MED/28 PO
and Stomatologic - UCOOS
Not assigned
MED/08 PO
Exclusive
Member
Member
PO
Director
UNIUD
Department of Materials and Natural INGResources - DMNR
IND/24
Not assigned
AGR/12
PA
Member
UNITS
Department of Life Sciences - DLS
RU
Exclusive
Member
UNITS
UNIPD
UNITS
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
BIO/06
PA
Exclusive
Member
Exclusive
Member
Member non
academic
Exclusive
Member
Member non
academic
Exclusive
Member
Exclusive
Member
Exclusive
Member
Member
foreing
University
Exclusive
Member
Exclusive
Member
Exclusive
Member
Member
UNICZ
INGIND/22
FIS/07
Ruolo
Collegio
Exclusive
Member
Exclusive
Member
130
Componente
Ente
Dipartimento
SSD
Qualifica
FORNASIERO
UNITS
Paolo
GAMINI Amelia UNITS
Department of Chemical Sciences DSCH
Department of Life Sciences - DLS
CHIM/03 PA
GIRALDI Tullio
UNITS
Department of Life Sciences - DLS
BIO/14
GRAZIANI
Mauro
LAZZARINO
Marco
LUGHI Vanni
UNITS
Department of Chemical Sciences DSCH
Not assigned
CHIM/03 PO
UNITS
INGIND/22
INGINF/01
BIO/06
RIC
MARSI Stefano
Department of Materials and Natural
Resources - DMNR
Department of Electronics,
Electrotecnics and Informatics - DEEI
Department of Life Sciences - DLS
Department of Materials and Natural INGResources - DMNR
IND/22
Department of Physics - DF
FIS/01
PO
Department of Mechanical
Engineering - DIME
Not assigned
PA
UNITS
MARZARI
UNITS
Roberto
MERIANI Sergio UNITS
MORGANTE
UNITS
Alberto
MUSCIA Roberto UNITS
ONESTI Silvia
Elettra
Synchrotron Spa
PAOLETTI Sergio UNITS
Department of Life Sciences - DLS
PASQUATO
UNITS
Department of Chemical Sciences Lucia
DSCH
PRATI Ubaldo
Fund. Tommaso Not assigned
Campanella
PRICL Sabrina
UNITS
Department of Materials and Natural
Resources - DMNR
RAVALICO
UNITS
Department of Anesthesia, Surgery,
Giuseppe
Pathological Anatomy, Dermatology,
Pathology and Molecular Sciences
ROITTI Sergio
UNITS
Department of Materials and Natural
Resources - DMNR
ROSEI Renzo
UNITS
Department of Physics - DF
RUBINI Silvia
INFM-TASC
SBAIZERO Orfeo UNITS
SCHMID Chiara UNITS
SERGO Valter
UNITS
CHIM/04 RU
RIC
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
RU
PO
PA
RIC
BIO/19 PO
CHIM/06 PA
P.Osp
INGPA
IND/24
MED/30 PO
INGIND/22
FIS/03
Not assigned
Department of Materials and Natural
Resources - DMNR
Department of Materials and Natural
Resources - DMNR
Department of Materials and Natural
PO
PA
PO
RIC
INGIND/22
INGIND/22
ING-
PO
PA
PO
Ruolo
Collegio
Exclusive
Member
Exclusive
Member
Exclusive
Member
Exclusive
Member
Member non
academic
Exclusive
Member
Exclusive
Member
Exclusive
Member
Exclusive
Member
Vice Director
Exclusive
Member
Member non
academic
Member
Exclusive
Member
Member non
academic
Exclusive
Member
Exclusive
Member
Exclusive
Member
Exclusive
Member
Member non
academic
Exclusive
Member
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Member
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131
Componente
Ente
SSD
Qualifica
CRO Aviano
Resources - DMNR
IND/22
Department of Anesthesia, Surgery, MED/08 PA
Pathological Anatomy, Dermatology,
Pathology and Molecular Sciences
Not assigned
P.Osp
UNIVE
Not assigned
STANTA Giorgio UNITS
TOFFOLI
Giuseppe
UGO Paolo
Dipartimento
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
CHIM/01 PA
Ruolo
Collegio
Member
Member
Member non
academic
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Member
132
8. Pubblicazioni rappresentative del collegio docenti
Estratto della produzione scientifica del collegio dal 2004 al 2009 del
personale dipendente dall’Università.
N Docente
1 BARALDI
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2 BARALDI
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Pubblicazione
BARALDI A. (2008). Structure and chemical reactivity of transition metal surfaces
as probed by synchrotron radiation core-level photoelectron spectroscopy.
JOURNAL OF PHYSICS. CONDENSED MATTER, vol. 20; p. 93001, ISSN: 0953-8984
BARALDI A., BIANCHETTIN L, VESSELLI E, DE GIRONCOLI S, LIZZIT S, PETACCIA L,
ZAMPIERI G, COMELLI G, ROSEI R (2007). Highly under-coordinated atoms at Rh
surfaces: interplay of strain and coordination effects on core level shift. NEW
JOURNAL OF PHYSICS, vol. 9; p. 143, ISSN: 1367-2630
BARALDI A., GIACOMELLO D, RUMIZ L, MORETUZZO M, LIZZIT S, BUATIER DE
MONGEOT F, PAOLUCCI G, COMELLI G, ROSEI R, NIEUWENHUYS B.E, VALBUSA U.
AND KISINOVA M (2005). Unexpected behavior of the surface composition of PtRh
alloys during chemical reactio. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,
vol. 127; p. 5671, ISSN: 0002-7863
BARALDI A., LIZZIT S, BONDINO F, COMELLI G, ROSEI R, SBRACCIA C, BONINI N,
BARONI S, MIKKELSEN A, ANDERSN J.N (2005). Thermal stability of the Rh(110)
missing-row reconstruction: combination of real-time corelevel spectroscopy and
ab initio modeling. PHYSICAL REVIEW. B, CONDENSED MATTER AND MATERIALS
PHYSICS, vol. 72; p. 75417, ISSN: 1098-0121
BARALDI A., LIZZIT S, COMELLIG, KISKINOVA M, ROSEI R, HONKALA K. AND
NORSKOV J.K (2004). A spectroscopic link between surface structure and local
chemical reactivity. PHYSICAL REVIEW LETTERS, vol. 93; p. 46101, ISSN: 0031-9007
BARALDI A., VESSELLI E, BIANCHETTIN L, COMELLI G, LIZZIT S, PETACCIA L, DE
GIRONCOLI S, LOCATELLI A, MENTES T.O, ABALLE L, WEISSENRIEDER J, ANDERSEN
J.N (2007). The (1x1) ? hexagonal structural transition on Pt(100) studied by highenergy resolution core level photoemission. THE JOURNAL OF CHEMICAL PHYSICS,
vol. 127; p. 164702, ISSN: 0021-9606
BIANCHETTIN L, BARALDI A., DE GIRONCOLI S, LIZZIT S, PETACCIA L, VESSELLI E,
COMELLI G, ROSEI R (2006). Geometric and electronic structure of the N/Rh(100)
system by core-level photoelectron spectroscopy: Experiment and Theory.
PHYSICAL REVIEW. B, CONDENSED MATTER AND MATERIALS PHYSICS, vol. 74; p.
45430, ISSN: 1098-0121
BIANCHETTIN L, BARALDI A., VESSELLI E, DE GIRONCOLI S, LIZZIT S, PETACCIA L,
COMELLI G, ROSEI R (2007). Experimental and theoretical Surface Core Level Shift
study of the S-Rh(100) local environment. JOURNAL OF PHYSICAL CHEMISTRY. C,
NANOMATERIALS AND INTERFACES, vol. 111; p. 4003, ISSN: 1932-7447
BUATIER DE MONGEOT F, TOMA A, MOLLE A, LIZZIT S, PETACCIA L, BARALDI A.
(2007). Self-organised synthesis of Rh nanostructures with tunable chemical
reactivity. NANOSCALE RESEARCH LETTERS, vol. 2; p. 251, ISSN: 1931-7573
DING X, DE ROGATIS L, VESSELLI E, BARALDI A., COMELLI G, ROSEI R, SAVIO L,
VATTUONE L, ROCCA M, ANCILOTTO F, BALDERESCHI A. AND PERESSI M (2007).
Interaction of carbon dioxide with Ni(110): a combined experimental and
theoretical study. PHYSICAL REVIEW. B, CONDENSED MATTER AND MATERIALS
PHYSICS, vol. 76; p. 195425, ISSN: 1098-0121
E. VESSELLI, L. BIANCHETTIN, BARALDI A., A. SALA, G. COMELLI, L. PETACCIA, S.
LIZZIT, S. DE GIRONCOLI (2008). The Ni3Al(111) surface structure: experiment and
theory. JOURNAL OF PHYSICS. CONDENSED MATTER, vol. 20; p. 195223, ISSN:
0953-8984
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12 BARALDI
Alessandro
13 BARALDI
Alessandro
14 BARALDI
Alessandro
15 BARALDI
Alessandro
16 BARALDI
Alessandro
17 BARALDI
Alessandro
18 BARALDI
Alessandro
19 BARALDI
Alessandro
20 BARALDI
Alessandro
21 BARALDI
Alessandro
22 BARALDI
Alessandro
23 BARALDI
Alessandro
24 BARALDI
Alessandro
25 BARALDI
Alessandro
E. VESSELLI, L. DE ROGATIS, X. DING, BARALDI A., L. SAVIO, L. VATTUONE, M.
ROCCA, P. FORNASIERO, M. PERESSI, A. BALDERESCHI, R. ROSEI AND G. COMELLI
(2008). Carbon Dioxide hydrogenation on Ni(110). JOURNAL OF THE AMERICAN
CHEMICAL SOCIETY, vol. 130; p. 11417, ISSN: 0002-7863
E. VESSELLI, M. CAMPANIELLO, BARALDI A., L. BIANCHETTIN, C. AFRICH, F. ESCH, S.
LIZZIT, G. COMELLI (2008). A surface core level shift study of hydrogen-induced
ordered structures on Rh(110). JOURNAL OF PHYSICAL CHEMISTRY. C,
NANOMATERIALS AND INTERFACES, vol. 112; p. 14475, ISSN: 1932-7447
F. BUATIER DE MONGEOT, A. TOMA, A. MOLLE, S. LIZZIT, L. PETACCIA, BARALDI A.
(2006). Carbon monoxide dissociation on Rh nanopyramids. PHYSICAL REVIEW
LETTERS, vol. 97; p. 56103, ISSN: 0031-9007
HOFMANN PH, ZAMPIERI G, PETACCIA L. LIZZIT S, BARALDI A. (2005). Comment on
"Momentum-dependent energy losses in core level photoemission spectra of
poorly conducting metals. PHYSICAL REVIEW LETTERS, vol. 94; p. 209703, ISSN:
0031-9007
L. BIANCHETTIN, BARALDI A., S. DE GIRONCOLI, E. VESSELLI, S. LIZZIT, G. COMELLI
AND R. ROSEI (2009). SURFACE CORE LEVEL SHIFT: HIGH SENSITIVE PROBE TO
OXYGEN-INDUCED RECONSTRUCTION OF RH(100). JOURNAL OF PHYSICAL
CHEMISTRY. C, NANOMATERIALS AND INTERFACES, vol. 113; p. 13192-13198,
ISSN: 1932-7447, doi: 10.1021/jp901223d
L. BIANCHETTIN, BARALDI A., S. DE GIRONCOLI, E. VESSELLI, S. LIZZIT, L. PETACCIA,
G. COMELLI, R. ROSEI (2008). Core level shifts of under-coordinated Pt atoms. THE
JOURNAL OF CHEMICAL PHYSICS, vol. 128; p. 114706, ISSN: 0021-9606
L. DE ROGATIS, E. VESSELLI, BARALDI A., M.F. CASULA, T. MONTINI, G. COMELLI,
M. GRAZIANI AND P. FORNASIERO (2009). Charge redistribution at the embedded
Rh-alumina interface. JOURNAL OF PHYSICAL CHEMISTRY. C, NANOMATERIALS
AND INTERFACES, vol. 113; p. 18069-18074, ISSN: 1932-7447
M. BIANCHI, D. CASSESE, A. CAVALLIN, R. COMIN, F. ORLANDO, L. POSTREGNA, E.
GOLFETTO, S. LIZZIT, BARALDI A. (2009). Clean and oxygen induced surface core
level shift on Ir(111). NEW JOURNAL OF PHYSICS, vol. 11; p. 063002, ISSN: 13672630
P. LACOVIG, M. POZZO, D. ALFÈ, P. VILMERCATI, BARALDI A., S. LIZZIT (2009).
Growth of dome-shaped carbon nanoislands on Ir(111): the intermediate
between carbidic clusters and quasi free-standing graphene. PHYSICAL REVIEW
LETTERS, vol. 103; p. 166101, ISSN: 0031-9007
S. LIZZIT, BARALDI A., CH. GRÜTTER, J.H. BILGRAM, PH. HOFMANN (2009). The
surface phase transition and low temperature phase of ?-Ga(010) studied by SPALEED. SURFACE SCIENCE, vol. 603; p. 3222, ISSN: 0039-6028
S. LIZZIT, Y. ZHANG, K.L. KOSTOV, L. PETACCIA, BARALDI A., R. LARCIPRETE, D.
MENZEL, K. REUTER (2009). Oand H- induced surface core level shifts on Ru(0001):
prevalence of the additivity rule. JOURNAL OF PHYSICS. CONDENSED MATTER, vol.
21; p. 134009, ISSN: 0953-8984
SANTUCCI S. C., GOLDONI A., LARCIPRETE R., LIZZIT S., BERTOLO M., BARALDI A.
(2004). Calorimetry at surfaces using high-resolution core level photoemission.
PHYSICAL REVIEW LETTERS, vol. 93; p. 106105, ISSN: 0031-9007
SAVIO L, GERBI A, VATTUONE L, BARALDI A., COMELLI G, ROCCA M (2006).
Monitoring super- and subsurface oxygen on Ag(210) by high energy resolution Xray photoelectron spectroscopy: Subsurface diffusion and segregation. JOURNAL
OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES,
INTERFACES & BIOPHYSICAL, vol. 110; p. 942, ISSN: 1520-6106
T. STAUDT, A. DESIKUSUMASTUTI, M. HAPPEL, E. VESSELLI, BARALDI A., S.
GARDONIO, S. LIZZIT, F. ROHR AND J. LIBUDA (2008). Modelling NOx storage
meterials: a high-resolution photoelectron spectroscopy study on the interaction
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
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of NO2 with Al2O3/NiAl(110) and BaO/Al2O3/NiAl(110). JOURNAL OF PHYSICAL
CHEMISTRY. C, NANOMATERIALS AND INTERFACES, vol. 112; p. 9835, ISSN: 19327447
26 BARALDI
TAIT S.L, WANG Y, LIN N, COSTANTINI G, BARALDI A., ESCH F, LIZZIT S, PETACCIA L,
Alessandro
KERN K (2008). Metal-Organic coordination interactions in Fe-Teraphthalic acid
networks on Cu(100). JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 130;
p. 2108-2113, ISSN: 0002-7863
27 BARALDI
VESSELLI E, DE ROGATIS L, BARALDI A., COMELLI G, ROSEI R, GRAZIANI M (2005).
Alessandro
Structural and kinetic effects on a simple catalytic reaction: oxygen reduction on
Ni(110). THE JOURNAL OF CHEMICAL PHYSICS, vol. 112; p. 144710, ISSN: 00219606
28 BARALDI
VESSELLI E., BARALDI A., BONDINO F., COMELLI G., PERESSI M. AND ROSEI R.
Alessandro
(2004). Coverage-dependent hydrogen adsorption site determination on Rh(100)
by means of high-resolution core-level spectroscopy. PHYSICAL REVIEW. B,
CONDENSED MATTER AND MATERIALS PHYSICS, vol. 70; p. 115404, ISSN: 10980121
29 BARALDI
VESSELLI E., BARALDI A., COMELLI G., LIZZIT S. AND ROSEI R. (2004). Ethanol
Alessandro
decomposition: C-C cleavage selectivity on Rh(111). CHEMPHYSCHEM, vol. 5; p.
1133, ISSN: 1439-4235
30 BARALDI
WEESTRATE K.J., BARALDI A., RUMIZ L., LIZZIT S., COMELLI G. AND ROSEI R. (2004).
Alessandro
Surface Core Level Shift study of the hydrogen interaction with Rh(111). SURFACE
SCIENCE, vol. 566-568; p. 486, ISSN: 0039-6028
31 BARALDI
WESTSTRATE C.J, BAKKER J.W, RIENKS E.D.L, LIZZIT S, PETACCIA L, BARALDI A.,
Alessandro
NIEUWENYUS B.E (2005). NH3 adsorption and decomposition on Ir(110): a
combined temperature programmed desorption and high resolution fast x-ray
phtoelectron spectroscopy study. THE JOURNAL OF CHEMICAL PHYSICS, vol. 122;
p. 184705, ISSN: 0021-9606
32 BARALDI
WESTSTRATE C.J, BAKKER J.W, RIENKS E.D.L, MARTINEZ J.R, VINOD C.P, LIZZIT S,
Alessandro
PETACCIA L, BARALDI A., NIEUWENHUYS B.E (2005). Selective NH3 oxidation on
(110) and (111) iridium surfaces. JOURNAL OF CATALYSIS, vol. 235; p. 92, ISSN:
0021-9517
33 BARALDI
WESTSTRATE C.J, BAKKER J.W, RIENKS E.D.L, VINOD C.P, LIZZIT S, PETACCIA L,
Alessandro
BARALDI A., NIEUWENHUYS B.E (2006). Synchrotron XPS and desorption study of
the NO chemistry on a stepped Pt surface. SURFACE SCIENCE, vol. 600; p. 1991,
ISSN: 0039-6028
34 BARALDI
WESTSTRATE C.J, BAKKER J.W, RIENKS E.D.L, VINOD C.P, LIZZIT S, PETACCIA L,
Alessandro
BARALDI A., NIEUWENYUS B.E (2005). The role of Oad in the decomposition of
NH3 adsorbed on Ir(110): a combined TPD and high-energy resolution fast XPS
study. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, vol. 7; p. 2629, ISSN: 1463-9076
35 BARALDI
ZAMPIERI G, LIZZIT S, PETACCIA L, GOLDONI A, BARALDI A., BREMHOLM M,
Alessandro
GAYONE J.E, HOFFMANN S, HOFMANN P (2005). Photoelectron diffraction study
of the 6H-SiC(0001) ? 3x ? 3 R30º reconstruction. PHYSICAL REVIEW. B,
CONDENSED MATTER AND MATERIALS PHYSICS, vol. 72; p. 165327, ISSN: 10980121
36 BONIN Serena BONIN S., BRUNETTI D, BENEDETTI E, DOTTI I, GORJI N, STANTA G (2008).
Molecular characterisation of breast cancer patients at high and low recurrence
risk. VIRCHOWS ARCHIV, vol. 452; p. 241-250, ISSN: 0945-6317, doi:
10.1007/s00428-007-0570-9
37 BONIN Serena BONIN S., BRUNETTI D, BENEDETTI E, GORJI N, STANTA G. (2006). Expression of
cyclin-dependent kinases and CDC25a phosphatase is related with recurrences
and survival in women with peri- and post-menopausal breast cancer. VIRCHOWS
ARCHIV, vol. 448; p. 539-544, ISSN: 0945-6317
38 BONIN Serena BONIN S., PETRERA F, ROSAI J, STANTA G (2005). DNA and RNA obtained from
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39 BONIN Serena
40 BONIN Serena
41 BONIN Serena
42 BONIN Serena
43 BONIN Serena
44 BONIN Serena
45 BONIN Serena
46 BONIN Serena
47 BONIN Serena
48 BONIN Serena
49 BONIN Serena
50 BRESCHI
Lorenzo
51 BRESCHI
Lorenzo
52 BRESCHI
Lorenzo
Bouin's fixed tissues. JOURNAL OF CLINICAL PATHOLOGY, vol. 58; p. 313-316,
ISSN: 0021-9746
BONIN S., PETRERA F., STANTA G. (2005). PCR AND RT-PCR ANALYSIS IN ARCHIVAL
POSTMORTEM TISSUES. In: UCHS J, PODDA M. ENCYCLOPEDIA OF DIAGNOSTIC
GENOMICS AND PROTEOMICS. p. 985-988, NEW YORK: MARCEL DEKKER INC
MIERTUSOVA S, BONIN S., TREVISAN G AND STANTA G (2004). Mycosis fungoides
is not associated with HCV virus infection. BRITISH JOURNAL OF DERMATOLOGY,
vol. 115; p. 1108-1110, ISSN: 0007-0963
NARDON E, DONADA M, BONIN S., DOTTI I, STANTA G (2009). Higher random
oligo concentration improves reverse transcription yield of cDNA from bioptic
tissues and quantitative RT-PCR reliability. EXPERIMENTAL AND MOLECULAR
PATHOLOGY, vol. 87; p. 146-151, ISSN: 0014-4800, doi:
10.1016/j.yexmp.2009.07.005
PAULUZZI P, BONIN S., GONZALEZ INCHAURRAGA MA, STANTA G, TREVISAN G
(2004). DETECTION OF SPIROCHETAL DNA SIMULTANEOUSLY IN SKIN BIOPSIES,
PERIPHERAL BLOOD AND URINE FROM PATIENTS WITH ERYTHEMA MIGRANS.
ACTA DERMATO-VENEREOLOGICA, vol. 84; p. 106-110, ISSN: 0001-5555
SCAGGIANE B, BONIN S., CRISTIANO L, SIRACUSANO S, STANTA G, DAPAS B,
GIANSANTE C, FIOTTI N AND GRASSI G (2008). Prostate Tumour-Inducing gene-1
analysis in human prostate cancer cells and tissue in relation to Mycoplasma
infection. CANCER INVESTIGATION, vol. 26; p. 800-808, ISSN: 0735-7907
SIRACUSANO S, NICCOLINI B, KNEZ R, TIBERIO A, BENEDETTI E, BONIN S.,
CICILIATO S, PAPPAGALLO GS, BELGRANO E, STANTA G (2005). The simultaneous
use of telomerase, cytokeratin 20 and CD4 for bladder cancer detection.
EUROPEAN UROLOGY, vol. 47; p. 327-333, ISSN: 0302-2838
STANTA G, CESCATO A, BONIN S., BARBAZZA R (2008). BIOETHICS
CONSIDERATIONS FOR MEDICAL RESEARCH IN HUMAN ARCHIVE TISSUES: THE
POINT OF VIEW OF THE RESEARCHER. VIRCHOWS ARCHIV, vol. 453; p. 117- 119,
ISSN: 0945-6317
STANTA G, POZZI MUCELLI S, PETRERA F, BONIN S. (2006). A novel fixative
improves opportunities of nucleic acids and proteomic analysis in human archive's
tissues. DIAGNOSTIC MOLECULAR PATHOLOGY, vol. 15; p. 115- 123, ISSN: 10529551
TOTHOVA SM, BONIN S., TREVISAN G, STANTA (2006). Mycosis fungoides: is it a
Borrelia burgdorferi-associated disease?. BRITISH JOURNAL OF CANCER, vol. 94; p.
879-883, ISSN: 0007-0920
TREVISAN G, ORTENZIO S, BONIN S. (2005). Late Lyme disease, chronic Lyme
disease and post-Lyme disease syndrome. Clinical and laboratory analysis.
GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA, vol. 140; p. 417-434,
ISSN: 0392-0488
TREVISAN G, PADOVAN C, SCAINI MT, CINCO M, FLORIS R, BONIN S. (2008).
Anetoderma Associated with Lyme Disease: A Case Report. ACTA DERMATOVENEREOLOGICA, vol. 88; p. 536-538, ISSN: 0001-5555, doi: 10.2340/000155550513
BERTACCI A, BARONI C, BRESCHI L., VENTURI M, PRATI C (2007). The influence of
smear layer in lateral channels filling. CLINICAL ORAL INVESTIGATIONS, vol. 11; p.
353-359, ISSN: 1432-6981
BRESCHI L., CADENARO M, ANTONIOLLI F, SAURO S, BIASOTTO M, PRATI C, TAY
FR, DI LENARDA R (2007). Polymerization kinetics of dental adhesives cured with
LED: correlation between extent of conversion and permeability. DENTAL
MATERIALS, vol. 23; p. 1066-1072, ISSN: 0109-5641
BRESCHI L., CADENARO M, ANTONIOLLI F, VISINTINI E, TOLEDANO M, DI LENARDA
R (2007). Extent of polymerization of dental bonding systems on bleached enamel.
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Lorenzo
54 BRESCHI
Lorenzo
55 BRESCHI
Lorenzo
56 BRESCHI
Lorenzo
57 BRESCHI
Lorenzo
58 BRESCHI
Lorenzo
59 BRESCHI
Lorenzo
60 BRESCHI
Lorenzo
61 BRESCHI
Lorenzo
62 BRESCHI
Lorenzo
63 BRESCHI
Lorenzo
64 BRESCHI
Lorenzo
65 BRESCHI
Lorenzo
66 BRESCHI
Lorenzo
67 BRESCHI
Lorenzo
AMERICAN JOURNAL OF DENTISTRY, vol. 20; p. 275-280, ISSN: 0894-8275
BRESCHI L., CAMMELLI F, VISINTINI E, MAZZONI A, VITA F, CARRILHO M,
CADENARO M, FOULGER S, TAY FR, PASHLEY D, DI LENARDA R (2009). Influence of
chlorhexidine concentration on the durability of etch-and-rinse dentin bonds: a
12-month in vitro study. JOURNAL OF ADHESIVE DENTISTRY, vol. 11; p. 191-198,
ISSN: 1461- 5185
BRESCHI L., MAZZONI A, DE STEFANO DORIGO E, FERRARI M (2009). Adhesion to
intraradicular dentin: a review. JOURNAL OF ADHESION SCIENCE AND
TECHNOLOGY, vol. 23; p. 1053-1083, ISSN: 0169-4243
BRESCHI L., MAZZONI A, PASHLEY DH, PASQUANTONIO G, RUGGERI A JR, SUPPA P,
MAZZOTTI G, DI LENARDA R, TAY FR (2006). Electric Impulse-Assisted Application
of Self-Etch Adhesives to Dentin. JOURNAL OF DENTAL RESEARCH, vol. 85; p.
1092-1096, ISSN: 0022-0345
BRESCHI L., MAZZONI A, RUGGERI A JR, CADENARO M, DI LENARDA R, DORIGO E
(2008). Dental adhesion review: aging and stability of the bonded interface.
DENTAL MATERIALS, vol. 24; p. 90-101, ISSN: 0109-5641
BRESCHI L., PRATI C, GOBBI P, PASHLEY DH, MAZZOTTI G, TETI G, PERDIGAO J.
(2004). Immunohistochemical analysis of the collagen fibrils included inside the
hybrid layer: a FEISEM study. OPERATIVE DENTISTRY, vol. 29; p. 538-546, ISSN:
0361-7734
CADENARO M, ANTONIOLLI F, SAURO S, TAY FR, DI LENARDA R, PRATI C,
BIASOTTO M, CONTARDO L, BRESCHI L.
CADENARO M, BIASOTTO M, SCUOR N, BRESCHI L., DAVIDSON CL, DI LENARDA R
(2008). Assessment of polymerization contraction stress of three composite
resins. DENTAL MATERIALS, vol. 24; p. 681-685, ISSN: 0109- 5641
CADENARO M, BRESCHI L., ANTONIOLLI A, MAZZONI A, DI LENARDA R (2006).
Influence of whitening on the degree of conversion of dental adhesives on dentin.
EUROPEAN JOURNAL OF ORAL SCIENCES, vol. 114; p. 257- 262, ISSN: 0909-8836
CADENARO M, BRESCHI L., ANTONIOLLI F, NAVARRA CO, MAZZONI A, TAY FR, DI
LENARDA R, PASHLEY DH (2008). Degree of conversion of resin blends in relation
to ethanol content and hydrophilicity. DENTAL MATERIALS, vol. 24; p. 1194-1200,
ISSN: 0109-5641
CADENARO M, BRESCHI L., NUCCI C, ANTONIOLLI F, VISINTINI E, PRATI C, MATIS
BA, DI LENARDA R (2008). Effect of two in-office whitening agents on enamel
surface in vivo: a morphological and non contact profilometric study. OPERATIVE
DENTISTRY, vol. 33; p. 127-134, ISSN: 0361-7734
CADENARO M, BRESCHI L., RUEGGEBERG FA, AGEE K, DI LENARDA R, TAY FR,
PASHLEY DH (2009). Effect of adhesive hydrophilicity and curing-time on the
permeability of resins bonded to water vs. ethanol-saturated acidetched dentin.
DENTAL MATERIALS, vol. 25; p. 39-47, ISSN: 0109-5641
CADENARO M, BRESCHI L., RUEGGEBERG FA, SUCHKO M, GRODIN E, AGEE K, DI
LENARDA R, TAY FR, PASHLEY DH (2009). Effects of residual ethanol on the rate
and degree of conversion of five experimental resins. DENTAL MATERIALS, vol. 25;
p. 621-628, ISSN: 0109-5641
CADENARO M, DELISE C, ANTONIOLLI F, NAVARRA OC, DI LENARDA R, BRESCHI L.
(2009). Enamel and Dentin Bond Strength Following Ozone Application. JOURNAL
OF ADHESIVE DENTISTRY, vol. 11; p. 287-292, ISSN: 1461- 5185
CADENARO M, MARCHESI G, ANTONIOLLI F, DAVIDSON C, DE STEFANO DORIGO E,
BRESCHI L. (2009). Flowability of composites is no guarantee for contraction stress
reduction. DENTAL MATERIALS, vol. 25; p. 649-654, ISSN: 0109-5641
CADENARO M, PASHLEY DH, MARCHESI G, CARRILHO M, ANTONIOLLI F, MAZZONI
A, TAY FR, DI LENARDA R, BRESCHI L. (2009). Influence of chlorhexidine on the
degree of conversion and E-modulus of experimental adhesive blends. DENTAL
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MATERIALS, vol. 25; p. 1269-1274, ISSN: 0109-5641
ARRILHO MR, TAY FR, DONNELLY AD, AGEE KA, TJÄDERHANE L, MAZZONI A,
BRESCHI L., FOULGER S, PASHLEY DH (2009). Host-derived loss of dentin matrix
stiffness associated with solubilization of collagen. JOURNAL OF BIOMEDICAL
MATERIALS RESEARCH. PART B, APPLIED BIOMATERIALS, vol. 90; p. 373-380, ISSN:
1552-4981
CARRILHO MRO, GERALDELI S, TAY FR, DE GOES M, CARVALHO RM, TJDERHANE L,
REIS AF, HEBLING J, MAZZONI A, BRESCHI L., PASHLEY DH (2007). In Vivo
Preservation of Hybrid Layer by Chlorhexidine. JOURNAL OF DENTAL RESEARCH,
vol. 86; p. 529-533, ISSN: 0022-0345
CARVALHO CA, CANTORO A, MAZZONI A, GORACCI C, BRESCHI L., FERRARI M
(2009). Effect of the ethanol application on post luting to intraradicular dentin.
INTERNATIONAL ENDODONTIC JOURNAL, vol. 42; p. 129-135, ISSN: 0143-2885
CARVALHO CA, MONTICELLI F, CANTORO A, BRESCHI L., FERRARI M (2009). Pushout bond strength of fiber posts luted with unfilled resin cement. JOURNAL OF
ADHESIVE DENTISTRY, vol. 11; p. 65-70, ISSN: 1461-5185
CHERSONI S, SUPPA P, BRESCHI L., TAY FR, PASHLEY DH, PRATI C. (2004). Water
movement in the hybrid layer after different dentin treatments. DENTAL
MATERIALS, vol. 20; p. 796-803, ISSN: 0109-5641
CHERSONI S, SUPPA P, GRANDINI S, GORACCI C, MONTICELLI F, YIU C, HUANG C,
PRATI C, BRESCHI L., FERRARI M, PASHLEY DH, TAY FR (2004). In vivo and in vitro
permeability of one-step self-etch adhesives. JOURNAL OF DENTAL RESEARCH, vol.
83; p. 459-464, ISSN: 0022-0345
CONTARDO L, DE LUCA M, BEVILACQUA L, BRESCHI L., DI LENARDA R (2007).
Influence of calcium hydroxide debris on the quality of endodontic apical seal.
MINERVA STOMATOLOGICA, vol. 56; p. 509-517, ISSN: 0026-4970
DEIMLING D, BRESCHI L., HOTH-HANNIG W, RUGGERI A, HANNIG C,
NEKRASHEVYCH Y, PRATI C, HANNIG M. (2004). Electron microscopic detection of
salivary alpha-amylase in the pellicle formed in situ. EUROPEAN JOURNAL OF
ORAL SCIENCES, vol. 112; p. 503-509, ISSN: 0909-8836
ERHARDT ACG, OSORIO R, PROENÇA JP, AGUILERA FA, OSORIO E, BRESCHI L.,
TOLEDANO M (2009). Influence of multiple adhesive coatings and extended
etching time on the bonding effectiveness of self-etch adhesives to dentin.
OPERATIVE DENTISTRY, ISSN: 0361-7734
FALCONI M, TETI G, ZAGO M, GALANZI A, BRESCHI L., PELOTTI S, RUGGERI A,
MAZZOTTI G (2009). Influence of a commercial tattoo ink on protein production in
human fibroblasts. ARCHIVES OF DERMATOLOGICAL RESEARCH, vol. 301; p. 539547, ISSN: 0340-3696
FALCONI M, TETI G, ZAGO M, PELOTTI S, BRESCHI L., MAZZOTTI G (2007). Effects
of HEMA on type I collagen protein in human gingival fibroblasts. CELL BIOLOGY
AND TOXICOLOGY, vol. 23; p. 313-322, ISSN: 0742-2091
FALCONI M, TETI G, ZAGO M, PELOTTI S, GOBBI P, BRESCHI L., MAZZOTTI G (2007).
Effect of fixative on chromatin structure and DNA detection. MICROSCOPY
RESEARCH AND TECHNIQUE, vol. 70; p. 599-606, ISSN: 1059-910X
FERRARI M, CARVALHO CA, GORACCI C, ANTONIOLLI F, MAZZONI A, MAZZOTTI G,
CADENARO M, BRESCHI L. (2009). Influence of luting material filler content on
post cementation. JOURNAL OF DENTAL RESEARCH, vol. 88; p. 951-956, ISSN:
0022-0345
FERRARI M, CONIGLIO I, MAGNI E, CAGIDIACO MC, GALLINA G, PRATI C, BRESCHI
L. (2008). How can droplets formation occur in endodontically treated teeth
during bonding procedures?. JOURNAL OF ADHESIVE DENTISTRY, vol. 10; p. 211218, ISSN: 1461-5185
FOSCHI F, NUCCI C, MONTEBUGNOLI L, MARCHIONNI S, BRESCHI L., MALAGNINO
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VA, PRATI C. (2004). SEM evaluation of canal wall dentine following use of Mtwo
and ProTaper NiTi rotary instruments. INTERNATIONAL ENDODONTIC JOURNAL,
vol. 37; p. 832-839, ISSN: 0143-2885
HIRAISHI N, BRESCHI L., PRATI C, FERRARI M, TAGAMI J, KING NM (2007).
Technique sensitivity associated with air-drying of HEMA-free, single-bottle, onestep self-etch adhesives. DENTAL MATERIALS, vol. 23; p. 498-505, ISSN: 0109-5641
KOMORI PC, PASHLEY DH, TJADERHANE L, BRESCHI L., MAZZONI A, DE GOES MF,
WANG L, CARRILHO MR (2009). Effect of 2% chlorhexidine digluconate on the
bond strength to normal vs caries-affected dentin. OPERATIVE DENTISTRY, vol. 43;
p. 157-165, ISSN: 0361-7734
MAI S, KIM YK, TOLEDANO M, BRESCHI L., LING JQ, PASHLEY DH, TAY FR (2009).
Phosphoric acid esters cannot replace polyvinylphosphonic acid as
phosphoprotein analogs in biomimetic remineralization of resin-bonded dentin.
DENTAL MATERIALS, vol. 25; p. 1230-1239, ISSN: 0109-5641
MALACARNE-ZANON J, PASHLEY DH, AGEE KA, FOULGER S, ALVES MC, BRESCHI L.,
CADENARO M, GARCIA FP, CARRILHO MR (2009). Effects of ethanol addition on
the water sorption/solubility and percent conversion of comonomers in model
dental adhesives. DENTAL MATERIALS, vol. 25; p. 1275-1284, ISSN: 0109-5641
MAZZONI A, MANNELLO F, TAY FR, TONTI G, PAPA S, MAZZOTTI G, DI LENARDA R,
PASHLEY DH, BRESCHI L. (2007). Zymographic analysis and characterization of
MMP-2 and -9 isoforms in human sound dentin. JOURNAL OF DENTAL RESEARCH,
vol. 86; p. 436-440, ISSN: 0022-0345
MAZZONI A, MARCHESI G, CADENARO M, MAZZOTTI G, DI LENARDA R, FERRARI
M, BRESCHI L. (2009). Push-out stress for fibre posts luted using different adhesive
strategies. EUROPEAN JOURNAL OF ORAL SCIENCES, vol. 117; p. 447-453, ISSN:
0909-8836
MAZZONI A, PASHLEY DH, NISHITANI Y, BRESCHI L., TJADERHANE L, TOLEDANO M,
PASHLEY EL, TAY FR (2006). Reactivation of quenched endogenous proteolytic
activities in phosphoric acid-etched dentine by etch-and-rinse adhesives.
BIOMATERIALS, vol. 27; p. 4470-4476, ISSN: 0142-9612
MAZZONI A, PASHLEY DH, RUGGERI A JR, VITA F, FALCONI M, DI LENARDA R,
BRESCHI L. (2008). Adhesion to chondroitinase ABC treated dentin. JOURNAL OF
BIOMEDICAL MATERIALS RESEARCH. PART B, APPLIED BIOMATERIALS, vol. 86B; p.
228-236, ISSN: 1552-4981
MAZZONI A, PASHLEY DH, TAY FR, GOBBI P, ORSINI G, RUGGERI A JR, CARRILHO
M, TJÄDERHANE L, DI LENARDA R, BRESCHI L. (2009). Immunohistochemical
identification of MMP-2 and MMP-9 in human dentin: Correlative FEI SEM/TEM
analysis. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A, vol. 88; p.
697-703, ISSN: 1549-3296
MAZZONI A, VISINTINI E, VITA F, PASQUANTONIO G, SABOIA V, RUGGERI A JR,
DORIGO E, BRESCHI L. (2009). ElectroBond improves dentin impregnation of etchand-rinse adhesives. JOURNAL OF ADHESIVE DENTISTRY, vol. 11; p. 27-33, ISSN:
1461-5185
MONTANARO L, CAMPOCCIA D, RIZZI S, DONATI ME, BRESCHI L., PRATI C,
ARCIOLA CR. (2004). Evaluation of bacterial adhesion of Streptococcus mutans on
dental restorative materials. BIOMATERIALS, vol. 25; p. 4457-4463, ISSN: 01429612
MONTANARO L, CERVELLATI M, CAMPOCCIA D, PRATI C, BRESCHI L., ARCIOLA CR.
(2005). No genotoxicity of a new nickel-free stainless steel. INTERNATIONAL
JOURNAL OF ARTIFICIAL ORGANS, vol. 28; p. 58-65, ISSN: 0391-3988
NAVARRA CO, CADENARO M, ARMSTRONG SR, JESSOP J, ANTONIOLLI F, SERGO V,
DI LENARDA R, BRESCHI L. (2009). Degree of conversion of Filtek Silorane Adhesive
System and Clearfil SE Bond within the hybrid and adhesive layer: An in situ
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Raman analysis. DENTAL MATERIALS, vol. 52; p. 1178-1185, ISSN: 0109-5641
NAVARRA CO, CADENARO M, CODAN B, MAZZONI A, SERGO V, DE STEFANO
DORIGO E, BRESCHI L. (2009). Degree of conversion and interfacial nanoleakage
expression of three one-step self-etch adhesives. EUROPEAN JOURNAL OF ORAL
SCIENCES, vol. 117; p. 463-469, ISSN: 0909-8836
NISHITANI Y, YOSHIYAMA M, WADGAONKAR B, BRESCHI L., MANNELLO N,
MAZZONI A, CARVALHO R, TJADERHANE L, TAY FR, PASHLEY DH (2006). Activation
of gelatinolytic/collagenolytic activity in dentin by selfetching adhesives.
EUROPEAN JOURNAL OF ORAL SCIENCES, vol. 114; p. 160-166, ISSN: 0909-8836
ORSINI G, RUGGERI A JR, MAZZONI A, NATO A, FALCONI M, DI LENARDA R, NANCI
A, BRESCHI L. (2008). Immunohistochemical Localization of DMP1 in Human
Dentin. EUROPEAN JOURNAL OF HISTOCHEMISTRY, vol. 52; p. 215-220, ISSN:
1121-760X
ORSINI G, RUGGERI A JR, MAZZONI A, PAPA V, MAZZOTTI G, DI LENARDA R,
BRESCHI L. (2007). Immunohistochemical identification of decorin and biglycan in
human dentin: a correlative FEI-SEM/TEM study. CALCIFIED TISSUE
INTERNATIONAL, vol. 81; p. 39-45, ISSN: 0171-967X
ORSINI G, RUGGERI A JR, MAZZONI A, PAPA V, PICCIRILLI M, FALCONI M, DI
LENARDA R, BRESCHI L. (2007). Immunohistochemical identification of type I and
type III collagen and chondroitin sulphate in human predentine: a correlative FEISEM/TEM study. INTERNATIONAL ENDODONTIC JOURNAL, vol. 40; p. 669-678,
ISSN: 0143-2885
PASHLEY DH, TAY FR, YIU CKY, HASHIMOTO M, BRESCHI L., ITO S. (2004). Collagen
degradation by host-derived enzymes during aging. JOURNAL OF DENTAL
RESEARCH, vol. 83; p. 216-221, ISSN: 0022-0345
PASQUANTONIO G, TAY FR, MAZZONI A, SUPPA P, RUGGERI A JR, FALCONI M, DI
LENARDA R, BRESCHI L. (2007). Electric device improves bonds of simplified etchand-rinse adhesives. DENTAL MATERIALS, vol. 23; p. 513-518, ISSN: 0109-5641
PRATI C, CHERSONI S, ACQUAVIVA GL, BRESCHI L., SUPPA P, TAY FR, PASHLEY DH.
(2005). Permeability of marginal hybrid layers in composite restorations. CLINICAL
ORAL INVESTIGATIONS, vol. 9; p. 1-7, ISSN: 1432-6981
PRATI C, NUCCI C, TOLEDANO M, GARCIAGODOY F, BRESCHI L., CHERSONI S
(2004). Microleakage and marginalhybrid layer formation of compomer
restorations. OPERATIVE DENTISTRY, vol. 29; p. 35-41, ISSN: 0361-7734
RUGGERI A JR, MONTEBUGNOLI L, MATTEUCCI A, ZINI N, SOLIMANDO L, SERVIDIO
D, SUPPA P, CADENARO M, COCCO L, BRESCHI L. (2005). Cyclosporin A specifically
affects nuclear PLC?1 in immunodepressed hearth transplant patients with
gingival overgrowth. JOURNAL OF DENTAL RESEARCH, vol. 84; p. 747-751, ISSN:
0022-0345
RUGGERI A JR, PRATI C, MAZZONI A, NUCCI S, DI LENARDA R, MAZZOTTI G,
BRESCHI L. (2007). Immunohistochemical analysis of type I collagen and
chondroitin 4/6 sulphate of root dentin. ARCHIVES OF ORAL BIOLOGY, vol. 52; p.
1-8, ISSN: 0003-9969
RUGGERI A, ORSINI G, MAZZONI A, NATO F, PAPA V, PICCIRILLI M, PUTIGNANO A,
MAZZOTTI G, DE STEFANO DORIGO E, BRESCHI L. (2009). Immunohistochemical
and biochemical assay of versican in human sound predentine/dentine matrix.
EUROPEAN JOURNAL OF HISTOCHEMISTRY, vol. 53; p. 125-134, ISSN: 1121-760X
SABOIA VPA, NATO F, MAZZONI A, ORSINI G, PUTIGNANO A, GIANNINI M,
BRESCHI L. (2008). Adhesion of a twostep etch-and-rinse adhesive on collagendepleted dentin. JOURNAL OF ADHESIVE DENTISTRY, vol. 10; p. 419-422, ISSN:
1461-5185
SABOIA VPA, SILVA FCFA, NATO F, MAZZONI A, CADENARO M, MAZZOTTI G,
GIANNI M, BRESCHI L. (2009). Analysis of differential ageing of the adhesive
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interface produced by a two-step etch-and-rinse adhesive. EUROPEAN JOURNAL
OF ORAL SCIENCES, vol. 117; p. 618-624, ISSN: 0909-8836
SANDRUCCI MA, NICOLIN V, BIASOTTO M, BRESCHI L., CASAGRANDE L, DI
LENARDA R, GRILL V (2004). The adhesion of Flow 2002 fibroblasts to titanium
implant materials is influenced by different surface topographies and is related to
the immunocytochemical expression of fibronectin. JOURNAL OF APPLIED
BIOMATERIALS & BIOMECHANICS, vol. 2; p. 169-176, ISSN: 1722-6899
SANDRUCCI MA, NICOLIN V, CASAGRANDE L, BIASOTTO M, BRESCHI L., DI
LENARDA R, SANCILIO S, GRILL V (2005). Preconditioning of dental alloys: analysis
of fibroblast proliferation and expression of fibronectin and chondroitin sulphate.
JOURNAL OF MATERIALS SCIENCE, vol. 40; p. 6233-6240, ISSN: 0022-2461
SANTOS J, CARRILHO MR, TERVAHARTIALA T, SORSA T, FERRAZ C, BRESCHI L.,
MAZZONI A, PASHLEY DH, TJÄDERHANE L (2009). Determination of matrix
metalloproteinases in human radicular dentin. JOURNAL OF ENDODONTICS, vol.
35; p. 686-689, ISSN: 0099-2399
SAURO S, WATSON TF, TAY FR, CHERSONI S, BRESCHI L., BERNARDI F, PRATI C
(2006). Water uptake of bonding systems applied on root dentin surfaces: SEM
and confocal microscopic study. DENTAL MATERIALS, vol. 22; p. 671-680, ISSN:
0109-5641
SAVARINO L, BRESCHI L., TEDALDI M, BALDINI N, PRATI C (2004). Ability of
restorative and fluoride releasing materials to prevent marginal dentin
demineralization. BIOMATERIALS, vol. 25; p. 1011-1017, ISSN: 0142-9612
SAVARINO L, GRECO M, BALDINI N, GIUNTI A, PISTONE M, MARCHIONNI S,
BRESCHI L., PRATI C (2008). Evaluation of restorative materials with a new
perfusion system. JOURNAL OF ADHESIVE DENTISTRY, vol. 10; p. 269-275, ISSN:
1461-5185
STACCHI C, ORSINI G, DI IORIO D, BRESCHI L., DI LENARDA R (2008). Clinical,
histologic and histomorphometric analyses of regenerated bone in maxillary sinus
augmentation using fresh frozen human bone allografts. JOURNAL OF
PERIODONTOLOGY, vol. 79; p. 1789-1796, ISSN: 0022-3492
SUPPA P, BRESCHI L., RUGGERI A, MAZZOTTI G, PRATI C, CHERSONI S, DI LENARDA
R, PASHLEY DH, TAY FR (2005). Nanoleakage within the hybrid layer: A correlative
FEISEM/TEM investigation. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH.
PART B, APPLIED BIOMATERIALS, vol. 73; p. 7-14, ISSN: 1552-4981
SUPPA P, RUGGERI A JR, TAY FR, PRATI C, BIASOTTO M, FALCONI M, PASHLEY DH,
BRESCHI L. (2006). Reduced antigenicity of type I collagen and proteoglycans in
sclerotic dentin. JOURNAL OF DENTAL RESEARCH, vol. 85; p. 133-137, ISSN: 00220345
TAY FR, LOUSHINE RJ, MONTICELLI F, WELLER NR, BRESCHI L., FERRARI M,
PASHLEY DH (2005). Effectiveness of resin-coated gutta-percha cones and a dualcured, hydrophilic methacrylate resin-based sealer in obturating root canals.
JOURNAL OF ENDODONTICS, vol. 31; p. 659-664, ISSN: 0099-2399
TAY FR, MAZZONI A, PASHLEY DH, DAY TE, NGOH EC, BRESCHI L. (2006). Potential
iatrogenic tetracycline staining of endodontically treated teeth via NaOCl/MTAD
irrigation - a preliminary report. JOURNAL OF ENDODONTICS, vol. 32; p. 354-358,
ISSN: 0099-2399
TETI G, MAZZOTTI G, ZAGO M, ORTOLANI M, BRESCHI L., PELOTTI S, RUGGERI A,
FALCONI M (2009). HEMA down-regulates procollagen ?1 type I in human gingival
fibroblasts. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A, vol. 90; p.
256-262, ISSN: 1549-3296
VENTURI M, BRESCHI L. (2004). Evaluation of apical filling after warm vertical
compaction using different procedures. JOURNAL OF ENDODONTICS, vol. 30; p.
436-440, ISSN: 0099-2399
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Milena
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VENTURI M, BRESCHI L. (2005). A comparison between two electronic apex
locators: an in vivo investigation. INTERNATIONAL ENDODONTIC JOURNAL, vol.
38; p. 36-45, ISSN: 0143-2885
VENTURI M, BRESCHI L. (2007). A comparison between two electronic apex
locators: an ex-vivo investigation. INTERNATIONAL ENDODONTIC JOURNAL, vol.
40; p. 362-373, ISSN: 0143-2885
VENTURI M, DI LENARDA R, BRESCHI L. (2006). An ex vivo comparison of three
different gutta-percha cones when compacted at different temperatures:
rheological considerations in relation to the filling of lateral canals.
INTERNATIONAL ENDODONTIC JOURNAL, vol. 31; p. 877-881, ISSN: 0143-2885
VENTURI M, DI LENARDA R, PRATI C, BRESCHI L. (2005). An in vitro model to
investigate filling of lateral canals. JOURNAL OF ENDODONTICS, vol. 31; p. 877881, ISSN: 0099-2399
VISINTINI E, MAZZONI A, VITA F, PASQUANTONIO G, CADENARO M, DI LENARDA
R, BRESCHI L. (2008). Effects of thermocycling on bonds created by simplified onestep self-etch adhesives applied with ElectroBond. EUROPEAN JOURNAL OF ORAL
SCIENCES, vol. 116; p. 564-570, ISSN: 0909-8836
YIU CKY, HIRAISHI N, CHERSONI S, BRESCHI L., FERRARI M, PRATI C, KING NNM,
PASHLEY DH, TAY FR (2006). Single-bottle adhesives behave as permeable
membranes after polymerisation. II. Differential permeability reduction with
oxalate desensitiser. JOURNAL OF DENTISTRY, vol. 34; p. 106-116, ISSN: 03005712
ZAGO M, TETI G, MAZZOTTI G, RUGGERI A JR, BRESCHI L., PELOTTI S, ORTOLANI M,
FALCONI M (2008). Expression of procollagen ?1 type I and tenascin proteins
induced by HEMA in human pulp fibroblasts. TOXICOLOGY IN VITRO, vol. 22; p.
1153-1159, ISSN: 0887-2333
BEVILACQUA L, BIASOTTO M, CADENARO M., DI LENARDA R., DORIGO E. (2005).
Reaction exothermia of 2 relining resins for temporary crowns. MINERVA
STOMATOLOGICA, vol. 54; p. 35-41, ISSN: 0026-4970
BEVILACQUA L, CADENARO M., SOSSI A, BIASOTTO M, DI LENARDA R (2007).
Influence of air abrasion and etching on enamel and adaptation of a dental
sealant. EUROPEAN JOURNAL OF PAEDIATRIC DENTISTRY, vol. 8(1); p. 25-30, ISSN:
1591-996X
BEVILACQUA L, SOSSI A, CADENARO M., DI LENARDA R (2007). Comparative
evaluation of the microhardness of 4 dental sealants. EUROPEAN JOURNAL OF
PAEDIATRIC DENTISTRY, vol. 8; p. 179-182, ISSN: 1591-996X
BIASOTTO M, DELLA PUTTA P, CADENARO M., SUPPA P, BRESCHI L, DI LENARDA R.
(2004). Efficacia della lampada a LED per la polimerizzazione delle resine
composite. GIORNALE ITALIANO DI CONSERVATIVA, vol. 2; p. 107-112, ISSN: 17242908
BRESCHI L, CADENARO M., ANTONIOLLI F, SAURO S, BIASOTTO M, PRATI C, TAY
FR, DI LENARDA R (2007). Polymerization kinetics of dental adhesives cured with
LED: correlation between extent of conversion and permeability. DENTAL
MATERIALS, vol. 23(9); p. 1066-1072, ISSN: 0109-5641
BRESCHI L, CADENARO M., ANTONIOLLI F, VISINTINI E, TOLEDANO M, DI LENARDA
R (2007). Extent of polymerization of dental bonding systems on bleached enamel.
AMERICAN JOURNAL OF DENTISTRY, vol. 20(4); p. 275-280, ISSN: 0894-8275
BRESCHI L, CAMMELLI F, VISINTINI E, MAZZONI A, VITA F, CARRILHO M,
CADENARO M., FOULGER S, MAZZOTI G, TAY FR, DI LENARDA R, PASHLEY D
(2009). Influence of chlorhexidine concentration on the durability of etch-andrinse
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427 FORNASIERO
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M (2007). Interaction of carbon dioxide with Ni(110): A combined experimental
and theoretical study. PHYSICAL REVIEW. B, CONDENSED MATTER AND
MATERIALS PHYSICS, vol. 76; p. 195425-1-195425-12, ISSN: 1098-0121
ESCH F, FABRIS S, ZHOU L, MONTINI T, AFRICH C, FORNASIERO P., COMELLI G,
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Substrates. SCIENCE, vol. 309; p. 752-755, ISSN: 0036-8075
FITTIPALDI M, GOMBAC V, MONTINI T, FORNASIERO P., GRAZIANI M (2008). A
High-Frequency (95 GHz) Electron Paramagnetic Resonance Study of B-doped
TiO2 photocatalysts. INORGANICA CHIMICA ACTA, vol. 361; p. 3980- 3987, ISSN:
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FORNASIERO P., A. SPEGHINI, R. DI MONTE, M. BETTINELLI, J. KAPAR, A. BIGOTTO,
V. SERGO AND M. GRAZIANI (2004). Laser excited luminescence of trivalent
lanthanide impurities and local structure in CeO2-ZrO2 mixed oxides. CHEMISTRY
OF MATERIALS, vol. 16; p. 1938-1944, ISSN: 0897-4756
FORNASIERO P., MONTINI T, DE ROGATIS L (2007). Catalysts Design for Hydrogen
Production: Embedded Rhodium Nanoparticles. Diffusion and Reactivity of Solids.
vol. -, p. 65-109, NEW YORK: Nova Science Publisher, ISBN/ISSN: 1-60021-890-3
FORNASIERO P., MONTINI T, GRAZIANI M, ZILLIO S, SUCCI M (2008). Development
of Catalytic Nanoceramicloaded Fe-Al-Cr Alloy Fiber Media for Exhaust Gas
Purification. CATALYSIS TODAY, vol. 137; p. 475-482, ISSN: 0920-5861, doi:
10.1016/j.cattod.2007.12.097.
GASPAROTTO A, BARRECA D, FORNASIERO P., GOMBAC V, LEBEDEV O.I,
MACCATO C, MONTINI T, TONDELLO E, VAN TENDELOO G, COMINI E,
SBERVEGLIERI G (2009). Multi-functional copper oxide nanosystems for H2
sustainable production and sensing. ECS TRANSACTIONS, vol. 25; p. 1169-1176,
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GENNARI F.C, MONTINI M, FORNASIERO P., ANDRADE GAMBOA J.J (2008).
REDUCTION BEHAVIOR OF NANOPARTICLES OF Ce0.8Zr0.2O2 PRODUCED BY
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GENNARI F.C, MONTINI T, HICKEY N, FORNASIERO P., GRAZIANI M (2006). IR
investigation of the interaction of deuterium with Ce0.6Zr0.4O2 and Cl-doped
Ce0.6Zr0.4O2. APPLIED SURFACE SCIENCE, vol. 252; p. 8456-8465, ISSN: 01694332
GENNARI F.C, NEYERTZ C, MAYER G, MONTINI T, FORNASIERO P. (2006). Hydrogen
adsorption kinetics on Pd/Ce0.8Zr0.2O2. PHYSICAL CHEMISTRY CHEMICAL
PHYSICS, vol. 8; p. 1-11, ISSN: 1463-9076
GENNARI F.C, NEYERTZ C, MEYER G, FORNASIERO P., GRAZIANI M (2005). Kinetics
of hydrogen chemisorption on high surface area Pd/Ce0.8Zr0.2O2. JOURNAL OF
ALLOYS AND COMPOUNDS, vol. 404-406; p. 317-322, ISSN: 0925-8388
GOMBAC V, DE ROGATIS L, GASPAROTTO A, VICARIO G, MONTINI T, BARRECA D,
BALDUCCI B, FORNASIERO P., TONDELLO E, GRAZIANI M (2007). TiO2
nanopowders doped with boron and nitrogen for photocatalytic applications.
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P. (2009). Photocatalytic Production of Hydrogen over Tailored Cu-Embedded
TiO2. NANOSCIENCE AND NANOTECHNOLOGY LETTERS, vol. 000; p. 000, ISSN:
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431 FORNASIERO GOMBAC V, SORDELLI L, MONTINI T, DELGADO J. J, ADAMSKI A, ADAMI G,
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CARGNELLO M, BERNAL S, FORNASIERO P. (2009). CuOx-TiO2 Photocatalysts for
H2 Production from Ethanol and Glycerol Solutions. JOURNAL OF PHYSICAL
CHEMISTRY. A, MOLECULES, SPECTROSCOPY, KINETICS, ENVIRONMENT, &
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432 FORNASIERO HAMEED A, GOMBAC V, MONTINI T, GRAZIANI M, FORNASIERO P. (2009).
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Synthesis, Characterization and Photocatalytic Activity of NiO-Bi2O3
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433 FORNASIERO HAMEED A, GOMBAC. V, MONTINI T, GRAZIANI M, FORNASIERO P. (2009).
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Photocatalytic Degradation of Dyes on NiO-Bi2O3 Nanocomposite.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, vol. 8; p. 677-682, ISSN: 1474905X, doi: 10.1039/b817396f
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Photocatalytic Activity Correlation of Bi2O3/Bi2O4-x Nanocomposite. JOURNAL OF
THE AMERICAN CHEMICAL SOCIETY, vol. 130; p. 9658-9659, ISSN: 0002-7863
435 FORNASIERO HICHEY N, ARNEODO LAROCHETTE P, GENTILINI C, SORDELLI L, OLIVI L, POLIZZI S,
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MONTINI T, FORNASIERO P., PASQUATO L, GRAZIANI M (2007). Monolayer
Protected Gold Nanoparticles on Ceria for an Efficient CO Oxidation Catalyst.
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436 FORNASIERO HICKEY N, FORNASIERO P., GRAZIANI M (2006). Hydrogen based technologies for
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mobile applications. Renewable Resources and Renewable Energy: A Global
Challenge. p. 225-272, NEW YORK: CRC Taylor and Francis, ISBN/ISSN: 978-0-84939689-2
437 FORNASIERO I. ALESSANDRI, M.A. BAARES, M. FERRONI, L.E. DEPERO, FORNASIERO P., F.C.
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GENNARI, N. HICKEY, M.V. MARTINEZ-HUERTA AND T. MONTINI (2006). Structural
investigation of Ce2Zr2O8 after redox treatments which lead to low temperature
reduction. TOPICS IN CATALYSIS, vol. 41; p. 35-42, ISSN: 1022-5528
438 FORNASIERO LEE S, BEVILACQUA M, FORNASIERO P., VOHS J. M, R.J. GORTE (2009). SOFC
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Cathodes Prepared by Infiltration of LNF and LSNF in Porous YSZ. JOURNAL OF
POWER SOURCES, vol. 193; p. 747-753, ISSN: 0378-7753, doi:
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439 FORNASIERO MONTINI T, BEVILACQUA M, FONDA E, CASULA M.F, LEE S, TAVAGNACCO C,
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GORTE R.J, FORNASIERO P. (2009). Relationship between electrical behavior and
structural characteristics in Sr-doped LaNi0.6Fe0.4O3-d mixed oxides. CHEMISTRY
OF MATERIALS, vol. 21; p. 1768-1774, ISSN: 0897-4756, doi: 10.1021/cm900467c
440 FORNASIERO MONTINI T, CONDO A, HICKEY N, LOVEY F.C, DE ROGATIS L, FORNASIERO P., AND
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GRAZIANI M (2007). Embedded Rh(1wt %)@Al2O3: Effects of high temperature
and prolonged aging under methane partial oxidation conditions. APPLIED
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441 FORNASIERO MONTINI T, DE ROGATIS L, GOMBAC V, FORNASIERO P., GRAZIANI M (2007).
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Rh(1%)@CexZr1-xO2-Al2O3 nanocomposites: active and stable catalysts for
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442 FORNASIERO MONTINI T, SPEGHINI A, DE ROGATIS L, LORENZUT B, BETTINELLI M, GRAZIANI M,
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FORNASIERO P. (2009). The identification of the structural phases of CexZr1-xO2
by Eu(III) luminescence studies. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,
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443 FORNASIERO MONTINI T, SPEGHINI A, FORNASIERO P., BETTINELLI M, GRAZIANI M (2008).
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Effect of the thermal pretreatments on ceria - zirconia redox properties: an Eu3+
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444 FORNASIERO N. HICKEY, FORNASIERO P., R. DI MONTE, J. KAPAR, J.R. GONZLEZ-VELASCO, M.A.
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GUTIRREZ-ORTIZ, M.P. GONZLEZ-MARCOS, J.M. GATICA, S. BERNAL (2004).
Reactivation of aged model Pd/Ce0.68Zr0.32O2 three-way catalyst by high
temperature oxidising treatment. CHEMICAL COMMUNICATIONS; p. 196-197,
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445 FORNASIERO NATILE M.M, POLETTO F, GALENDA A, GLISENTI A, MONTINI T, DE ROGATIS T,
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FORNASIERO P. (2008). La0.6Sr0.4Co1-yFeyO3-d perovskites: influence of the
Co/Fe atomic ratio on properties and catalytic activity towards alcohol steamreforming. CHEMISTRY OF MATERIALS, vol. 20; p. 2314-2327, ISSN: 0897-4756,
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446 FORNASIERO R. DI MONTE, FORNASIERO P., S. DESINAN, J. KASPAR, J. M. GATICA, J.J. CALVINO,
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E. FONDA (2004). Thermal stabilization of CexZr1-xO2 oxygen storage promoters
by addition of Al2O3: effect of thermal ageing on textural, structural and
morphological properties. CHEMISTRY OF MATERIALS, vol. 16; p. 4273-4285, ISSN:
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447 FORNASIERO ROSSIN A, IENCO A, COSTANTINO F, MONTINI, T, DI CREDICO B, CAPORALI M,
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GONSALVI L, FORNASIERO P., PERUZZINI M (2008). Crystal-to-crystal phase
transition and CO2 adsorption properties of polymeric magnesium formate.
CRYSTAL GROWTH & DESIGN, vol. 8; p. 3302-3308, ISSN: 1528-7483
448 FORNASIERO SHAH P.R, KIM T, ZHOU G, FORNASIERO P., GORTE R.J (2006). Evidence for
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entropy effects in the reduction of ceria-zirconia solutions. CHEMISTRY OF
MATERIALS, vol. 18; p. 5363-5369, ISSN: 0897-4756
449 FORNASIERO SIVASAMY A, CHEAH K.Y, FORNASIERO P., KEMAUSUOR F, ZINOVIEV S, MIERTUS S
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(2009). Catalytic Applications in Biodiesel Production from Vegetable Oils: A
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450 FORNASIERO SPECCHIA S, VELLA L.D, DE ROGATIS L, MONTINI T, SPECCHIA V, FORNASIERO P.
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(2009). Rh-based Catalysts for Syngas Production via SCT-CPO Reactors. CATALYSIS
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(2009). Effect of the Catalyst Load on Syngas Production in Short Contact Time
Catalytic Partial Oxidation Reactors. INDUSTRIAL & ENGINEERING CHEMISTRY
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AND M. GRAZIANI (2004). Promotion of reduction in Ce0.5Zr0.5O2: the pyrochlore
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453 FORNASIERO T. MONTINI, N. HICKEY, FORNASIERO P., M. GRAZIANI, M.A. BAARES, M.V.
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MARTINEZ-HUERTA, I. ALESSANDRI, AND L.E. DEPERO (2005). Variations in the
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454 FORNASIERO VESSELLI E, DE ROGATIS L, DING X, BARALDI A, SAVIO L, VATTUONE L, ROCCA M,
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FORNASIERO P., PERESSI M, BALDERESCHI A, ROSEI R, AND COMELLI G (2008).
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GOMBAC V, DE ROGATIS L, GASPAROTTO A, VICARIO G, MONTINI T, BARRECA D,
BALDUCCI B, FORNASIERO P, TONDELLO E, GRAZIANI M. (2007). TiO2
nanopowders doped with boron and nitrogen for photocatalytic applications.
CHEMICAL PHYSICS. vol. 339, pp. 111-123 ISSN: 0301-0104.
HICKEY N, LAROCHETTE PA, GENTILINI C, SORDELLI L, OLIVI L, POLIZZI T, MONTINI
T, FORNASIERO P, PASQUATO L, GRAZIANI M. (2007). Monolayer protected gold
particles on ceria for an efficient CO oxidation catalyst. CHEMISTRY OF
MATERIALS. vol. 19, pp. 650-651 ISSN: 0897-4756.
MONTINI T, BANARES M.A, HICKEY N, DI MONTE R, FORNASIERO P, KASPAR J,
GRAZIANI M. (2004). Promotion of reduction in Ce0.5Zr0.5O2: the pyrochlore
structure as effect rather than cause?. PHYSICAL CHEMISTRY CHEMICAL PHYSICS.
vol. 6, pp. 1-3 ISSN: 1463-9076.
MONTINI T, CONDO' AM, HICKEY T, DE ROGATIS L, FORNASIERO P, GRAZIANI M.
(2007). Embedded Rh(1wt%) @Al2O3: Effect of high temperture and prolonged
aging under methane partial oxidation conditions. APPLIED CATALYSIS. B,
ENVIRONMENTAL. vol. 73, pp. 84-97 ISSN: 0926-3373.
MONTINI T, DE ROGATIS L, GOMBAC V, FORNASIERO P, GRAZIANI M. (2007).
Rh(1%)CexZr1-xO2-Al2O3 nanocomposites: active and stable catalysts for ethanol
steam reforming. APPLIED CATALYSIS. B, ENVIRONMENTAL. vol. 71, pp. 125-134
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MONTINI T, HICKEY N, FORNASIERO P, GRAZIANI M., BANARES M.A, ALESSANDRI I,
DEPERO L.E. (2005). Variations in the extent of pyrochlore-type cation ordering in
Ce2Zr2O8: a t' - k pathway to low temperature reduction. CHEMISTRY OF
MATERIALS. vol. 17, pp. 1157-1166 ISSN: 0897-4756.
MONTINI T, SPEGHINI A, BETTINELLI M, GRAZIANI M. (2008). Effect of the thermal
pre-treatments on ceriazirconia redox properties: an Eu3+ luminescence study.
JOURNAL OF ALLOYS AND COMPOUNDS. vol. 451, pp. 617- 620 ISSN: 0925-8388.
SERPE A, BIGOLI F, CABRAS M.C, FORNASIERO P, GRAZIANI M., MERCURI M.L,
MONTINI T, PILIA L, TROGU E.F, DEPLANO P. (2005). Mild and safe one-step metal
palladium dissoluition and its application for an effective and selective Pdrecovery from spent catalytic converters. CHEMICAL COMMUNICATIONS. vol. nn,
pp. 1040-1042 ISSN: 1359-7345.
VESSELLI E, DE ROGATIS L, BARALDI A, COMELLI G, GRAZIANI M., ROSEI R. (2005).
Structural and kinetic effect on a simple catalytic reaction: oxygen reduction on
Ni(110). JOURNAL OF CHEMICAL PHYSICS. vol. 122 ISSN: 0021-9606. Articolo n.
144710.
BOESHORE SE, PARKER ER, LUGHI V., MACDONALD NC, BINGERT M (2005).
Aluminum nitride thin films on titanium for piezoelectric microelectromechanical
systems. In: 2005 Proceedings - IEEE Ultrasonics Symposium 3, p. 1641
CALLAGHAN LA, LUGHI V., MACDONALD NC, CLARKE DR (2006). Beam-supported
AlN thin film bulk acoustic resonators. IEEE TRANSACTIONS ON ULTRASONICS
FERROELECTRICS AND FREQUENCY CONTROL, vol. 53; p. 1001, ISSN: 0885-3010
CALLAGHAN LA, LUGHI V., REQUA MV, MACDONALD NC, CLARKE DR, TURNER KL
(2005). Fabrication and testing of beam supported AlN FBARs. In: 2005
Proceedings of the IEEE International Frequency Control Symposium and
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HIIE J, MUSKA K, VALDNA V, MIKLI V, LUGHI V., QUINCI F, SERGO V (in stampa).
Chlorine doping of CdS in chemical bath deposition process and effects of
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annealing on structural and electrical properties of the films. THIN SOLID FILMS,
ISSN: 0040-6090
LUGHI V. (2008). Metodo per la realizzazione di un materiale fotovoltaico. ?.
Under PCT process review
LUGHI V. (2009). Fisica della conversione fotovoltaica. Manuale di Energia Solare.
Tecniche Nuove
LUGHI V., CLARKE DR (2007). Defect and stress characterization of AlN films by
Raman spectroscopy. APPLIED PHYSICS LETTERS, vol. 89; p. 241911, ISSN: 00036951
LUGHI V., CLARKE DR (2007). Low-temperature transformation kinetics of
electron-beam deposited 5 wt.% yttriastabilized zirconia. ACTA MATERIALIA, vol.
55; p. 2049, ISSN: 1359-6454
LUGHI V., FERLUGA A, SERGO V, ROITTI S (2008). Crescita per bagno chimico e
caratterizzazione di film sottili di CdS per applicazioni fotovoltaiche. In: Atti del 9°
convegno nazionale AIMAT, p. 425
A. GREGORIO, S. CARRATO, T. BERNARDI, I. KOSTADINOV, MARSI S., M.
MESSEROTTI, AND R. STALIO (2004). AtmoCube: Observation of the earth
atmosphere from the space to study `space weather' effects,. In: 4S Symposium Small Satellites Systems & Services, Sept.2004
A. RESTREPO, MARSI S., G. RAMPONI (2008). CHROMATIC ENHANCEMENT FOR
THE VIRTUAL RESTORATION OF ART OBJECTS. In: Electronic Imaging & Visual Art
(EVA). Firenze, 16-18 aprile 2008, FIRENZE: Cappellini
A. UKOVICH, MARSI S., AND S. CARRATO (2004). Feasibility study of the real-time
implementation of an algorithm for high dynamics video data on a low-cost FPG.
In: 7th COST 276 Workshop
G. IMPOCO, MARSI S., G. RAMPONI (2005). Adaptive reduction of the dynamics of
HDR video sequences. In: IEEE Intern. Conf. on Image Processing (ICIP-05),
Sept.11-14
GUARNIERI G., MARSI S., RAMPONI G. (2006). Fast bilateral filter for edgepreserving smoothing. ELECTRONICS LETTERS, vol. 42; p. 25-26, ISSN: 0013-5194
MARSI S., G. IMPOCO, A. UKOVICH, S. CARRATO, G. RAMPONI (2007). Video
Enhancement and Dynamic range Control of HDR Sequences for Automotive
Applications. EURASIP JOURNAL ON ADVANCES IN SIGNAL PROCESSING, vol.
Volume 2007, ISSN: 1687-6172, doi: 10.1155/2007/80971
MARSI S., G. RAMPONI, AND S. CARRATO (2004). Image Contrast Enhancement
Using a Recursive Rational Filter. In: IEEE Int. Workshop on Imaging Systems and
Techniques, IST 2004
MARSI S., IMPOCO G, UKOVICH A, RAMPONI G, CARRATO S (2008). Using a
Recursive Rational Filter to Enhance Color Images. IEEE TRANSACTIONS ON
INSTRUMENTATION AND MEASUREMENT, vol. 57; p. 1230-1236, ISSN: 0018-9456,
doi: 10.1109/TIM.2007.915141
MARSI S., RAFFIN MARIO IT, CARRAI PAOLA IN (2007). A FILTER FOR RETRACTING
A LOCAL ADAPTATION LUMINANCE. WO2007026304. KONINKL PHILIPS
ELECTRONICS NV (NL); MARSI STEFANO (IT); RAFFIN MARIO (IT); CARRAI PAOLA
(IN). Classification: - international: G06T5/00; G06T5/20; G06T5/00; G06T5/20; European: G06T5/00D; G06T5/20 Application number: WO2006IB52973
20060828 Priority number(s): EP20050108053 200509
MARSI S., S. CARRATO, G. RAMPONI, AND B.CRESPI (2004). Video dynamic range
compression for logarithmic CMOS imagers. In: 6th COST 276 Workshop, May
2004, p. 28-33
S. SAPONARA, L. FANUCCI, MARSI S., G. RAMPONI (2007). Algorithmic and
Architectural Design for Realtime and Power-efficient Retinex Image/Video
Processing. JOURNAL OF REAL-TIME IMAGE PROCESSING, vol. 1, n.ro 4; p. 267-
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283, ISSN: 1861-8200, doi: 10.1007/s11554-007-0027-z
S. SAPONARA, L. FANUCCI, MARSI S., G. RAMPONI, D. KAMMLER, AND E. M.
WITTE (2007). Application-Specific Instruction-Set Processor for Retinex-Like
Image and Video Processing. IEEE TRANSACTIONS ON CIRCUITS AND SYSTEMS. II,
EXPRESS BRIEFS, vol. 54 n.ro 7; p. 596-600, ISSN: 1549-7747
S. SAPONARA, L. FANUCCI, MARSI S., G. RAMPONI, M. WITTE, D. KAMMLER
(2006). Design of Application Specific Instruction-set Processor for Image and
Video Filtering. In: EUSIPCO-06, 14th European Signal Processing Conf. Florence,
Italy, 4-8 Sept.
S. SAPONARA, M. CASSIANO, MARSI S., R. COEN, L. FANUCCI (2005). Cost-effective
VLSI Design of Non Linear Image Processing Filters. In: 8th Euromicro Conference
on Digital System Design (DSD05), 27 august - 3 september, p. 322-329
AZZONI, E, D. SBLATTERO, M. LICCIULLI, MARZARI R., AND P. EDOMI (2007). Using
archaeal histones for precise DNA fragmentation. PROTEIN ENGINEERING, DESIGN
& SELECTION, vol. 7; p. 46-56, ISSN: 1741-0126
BALDAS V, NOT T, TOMMASINI A, ANSALDI F, DEMARINI S, SBLATTERO D,
MARZARI R., TORELLI L, BURLINA A, TIRIBELLI C, VENTURA A. (2004). Antitransglutaminase antibodies and age. CLINICAL CHEMISTRY, vol. 50; p. 1856-1860,
ISSN: 0009-9147
BALDAS V., TOMMASINI A., SANTON D., NOT T., GERARDUZZI T., CLARICH G.,
SBLATTERO D., MARZARI R., FLORIAN F., MARTELLOSSI S., VENTURA A. (2004).
Testing for anti-human transglutaminase antibodies in saliva is not useful for
diagnosis of celiac disease. CLINICAL CHEMISTRY, vol. 50; p. 216-219, ISSN: 00099147
BARONE, M. V, I. CAPUTO, M. T. RIBECCO, M. MAGLIO, MARZARI R., D.
SBLATTERO, R. TRONCONE, S. AURICCHIO, AND C. ESPOSITO (2007). Humoral
immune response to tissue transglutaminase is related to epithelial cell
proliferation in celiac disease. GASTROENTEROLOGY, vol. 132; p. 1245-1253, ISSN:
0016-5085
BOSCOLO, S, A. SARICH, A. LORENZON, M. PASSONI, V. RUI, M. STEBEL, D.
SBLATTERO, MARZARI R., M. HADJIVASSILIOU, AND E. TONGIORGI (2007). Gluten
ataxia: passive transfer in a mouse model. ANNALS OF THE NEW YORK ACADEMY
OF SCIENCES, vol. 1107; p. 319-328, ISSN: 0077-8923
BRADBURY AR, VELAPPAN N, VERZILLO V, OVECKA M, MARZARI R., SBLATTERO D,
CHASTEEN L, SIEGEL R, PAVLIK P. (2004). Antibodies in proteomics. METHODS IN
MOLECULAR BIOLOGY, vol. 248; p. 519-546, ISSN: 1064-3745
DI NIRO R, DANGELO S, SECCO P, MARZARI R., SANTORO C, SBLATTERO D (2009).
Profiling the Autoantibody Repertoire by Screening Phage-Displayed Human cDNA
Libraries. METHODS IN MOLECULAR BIOLOGY, vol. 570; p. 353-369, ISSN: 10643745
DI NIRO, R, D. SBLATTERO, F. FLORIAN, M. STEBEL, L. ZENTILIN, M. GIACCA, V.
VILLANACCI, A. GALLETTI, T. NOT, A. VENTURA, MARZARI R. (2008). Anti-idiotypic
response in mice expressing human autoantibodies. MOLECULAR IMMUNOLOGY,
vol. 45; p. 1782-1791, ISSN: 0161-5890
DI NIRO, R, F. ZILLER, F. FLORIAN, S. CROVELLA, M. STEBEL, M. BESTAGNO, O.
BURRONE, A. R. BRADBURY, P. SECCO, MARZARI R., AND D. SBLATTERO (2007).
Construction of miniantibodies for the in vivo study of human autoimmune
diseases in animal models. BMC BIOTECHNOLOGY, vol. 7; p. 46-56, ISSN: 14726750
DI NIRO, R., F. FERRARA, T. NOT, A. R. BRADBURY, F. CHIRDO, MARZARI R., D.
SBLATTERO (2005). Characterizing monoclonal antibody epitopes by filtered gene
fragment phage display. BIOCHEMICAL JOURNAL, vol. 388; p. 889, ISSN: 02646021
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Roberto
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Roberto
FISCHETTI, F, P. DURIGUTTO, P. MACOR, MARZARI R., R. CARRETTA, AND F.
TEDESCO (2007). Selective therapeutic control of C5a and the terminal
complement complex by anti-C5 single-chain Fv in an experimental model of
antigen-induced arthritis in rats. ARTHRITIS AND RHEUMATISM, vol. 56; p. 11871197, ISSN: 0004-3591
KATOUZIAN, F., D. SBLATTERO, T. NOT, A. TOMMASINI, E. GIUSTO, D. MEIACCO,
M. STEBEL, MARZARI R., A. FASANO, A. VENTURA (2005). Dual sugar gutpermeability testing on blood drop in animal models. CLINICA CHIMICA ACTA, vol.
352; p. 191, ISSN: 0009-8981
MACOR P, TRIPODO C, ZORZET S, PIOVAN E, BOSSI F, MARZARI R., AMADORI A,
TEDESCO F (2007). In vivo targeting of human neutralizing antibodies against CD55
and CD59 to lymphoma cells increases the antitumor activity of rituximab.
CANCER RESEARCH, vol. 67; p. 10556-10563, ISSN: 0008-5472
MAGLIO M, FLORIAN F, VECCHIET M, AURICCHIO R, PAPARO F, SPADARO R, ZANZI
D, RAPACCIUOLO L, FRANZESE A, SBLATTERO D, MARZARI R., TRONCONE R (2009).
Majority of children with type 1 diabetes produce and deposit anti-tissue
transglutaminase antibodies in the small intestine. DIABETES, vol. 58; p. 17581784, ISSN: 0012-1797
MARZARI R., A. BRADBURY (2004). A method for selecting phage display open
reading frames on phages. WO 2004/001036. Università di California - U.S.A.
MARZARI R., F.TEDESCO (2005). Antibodies anti-c5 component of the complement
system and their use. US2006115476. Università di Trieste
MARZARI R., SBLATTERO D (2006). "Recombinant antibodies against human type ii
transglutaminase and uses thereof". WO2006100679. Quark Biotech Inc.
NEMEC, G, A. VENTURA, M. STEFANO, G. DI LEO, V. BALDAS, A. TOMMASINI, F.
FERRARA, A. TADDIO, A. CITTA, D. SBLATTERO, MARZARI R., AND T. NOT (2006).
Looking for celiac disease: diagnostic accuracy of two rapid commercial assays.
AMERICAN JOURNAL OF GASTROENTEROLOGY, vol. 101; p. 1597-1600, ISSN:
0002-9270
PAVLIK P, SIEGEL RW, MARZARI R., SBLATTERO D, VERZILLO V, COLLINS C, MARKS
JD, BRADBURY A. (2004). Predicting antigenic peptides suitable for the selection of
phage antibodies. JOURNAL OF BIOTECHNOLOGY, vol. 12; p. 99-112, ISSN: 01681656
SBLATTERO D., FLORIAN F., AZZONI D., ZIBERNA F., TOMMASINI A., NOT T.,
VENTURA A., BRADBURY A., MARZARI R. (2004). One-step cloning of anti tissue
transglutaminase scFv from subjects with celiac disease. JOURNAL OF
AUTOIMMUNITY, vol. 22; p. 65-72, ISSN: 0896-8411
SBLATTERO D., VENTURA A., TOMMASINI, A., CATTIN L., MARTELOSSI, S., FLORIAN
F., MARZARI R., R., BRADBURY A., NOT. T. (2006). Cryptic gluten intolerance in
type 1 diabetes: identifying suitable candidates for gluten-free diet. GUT, vol. 55;
p. 133-134, ISSN: 0017-5749
SBLATTERO, D., F. FLORIAN, T. NOT, A. VENTURA, R. TRONCONE, S. AURICCHIO,
MARZARI R. (2005). The Gut as Site of Production of Autoimmune Antibodies.
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, vol. 39s; p. 730,
ISSN: 0277-2116
SBLATTERO, D., F. MAURANO, G. MAZZARELLA, M. ROSSI, S. AURICCHIO, F.
FLORIAN, F. ZIBERNA, A. TOMMASINI, T. NOT, A. VENTURA, A. BRADBURY,
MARZARI R., R. TRONCONE (2005). Characterization of the antitissue
transglutaminase antibody response in nonobese diabetic mice. JOURNAL OF
IMMUNOLOGY, vol. 174; p. 5830, ISSN: 0022-1767
SECCHIERO P, SBLATTERO D, CHIARUTTINI C, MELLONI E, MACOR P, ZORZET S,
TRIPODO C, TEDESCO F, MARZARI R., ZAULI G (2009). Selection and
characterization of a novel agonistic human recombinant anti-TRAILR2 minibody
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547 MERIANI
Sergio
548 MERIANI
Sergio
549 MERIANI
Sergio
550 MERIANI
Sergio
551 MORGANTE
Alberto
552 MORGANTE
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Alberto
with anti-leukemic activity. INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY
AND PHARMACOLOGY, vol. 22; p. 73-83, ISSN: 0394-6320
SECCO P, E. D'AGOSTINI, MARZARI R., M. LICCIULLI, R.DI NIRO, S. D'ANGELO, A.R.
BRADBURY, U. DIANZANI, C. SANTORO, D. SBLATTERO (2009). Antibody library
selection by the {beta}-lactamase protein fragment complementation assay.
PROTEIN ENGINEERING, DESIGN & SELECTION, ISSN: 1741-0126
TOMMASINI, A., T. NOT, V. KIREN, V. BALDAS, D. SANTON, C. TREVISIOL, I. BERTI,
E. NERI, T. GERARDUZZI, I. BRUNO, A. LENHARDT, E. ZAMUNER, A. SPANO, S.
CROVELLA, S. MARTELLOSSI, G. TORRE, D. SBLATTERO, MARZARI R., A. BRADBURY,
G. TAMBURLINI, A. VENTURA (2004). Mass screening for coeliac disease using
antihuman transglutaminase antibody assay. ARCHIVES OF DISEASE IN
CHILDHOOD, vol. 89; p. 512-515, ISSN: 0003-9888
VENTURA A, NOT T, TOMMASINI A, MARZARI R., SBLATTERO D. (2004).
Autoantibodies as predictors of disease. LANCET, vol. 363; p. 1544-1546, ISSN:
0140-6736
VENTURA, A., T. NOT, A. TOMMASINI, MARZARI R., D. SBLATTERO (2005).
Autoantibodies as predictors of disease. LANCET, vol. 364; p. 1403, ISSN: 01406736
ZILLER, F., P. MACOR, R. BULLA, D. SBLATTERO, MARZARI R., F. TEDESCO (2005).
CONTROLLING COMPLEMENT RESISTANCE IN CANCER BY USING HUMAN
MONOCLONAL ANTIBODIES THAT NEUTRALIZE COMPLEMENTREGULATORY
PROTEINS. EUROPEAN JOURNAL OF IMMUNOLOGY, vol. 35; p. 2175, ISSN: 00142980
CLEMENTE D.A., LUCCHINI E., MERIANI S., FURLAN N. (2005). Crystal structure of
CeO2-ZrO2-Ta2O5 ternary system studued by Rietveld method. JOURNAL OF THE
EUROPEAN CERAMIC SOCIETY, vol. 25; p. 1863-1876, ISSN: 0955-2219, doi:
10.1016/j.jeurceramsoc.2004.06.002
M. ZAMMARANO, M. FRANCESCHI, S. BELLAYER, J.F. GILMAN, MERIANI S. (2005).
Preparation and flame resistance properties of revolutionary self-extinguishing
epoxy nanocomposites based on layered double hydroxides. POLYMER, vol. 46; p.
9314-9328, ISSN: 0032-3861, doi: 10.1016/j.polymer.2005.07.050
M. ZAMMARANO, S. BELLAYER, J. W. GILMAN, M.FRANCESCHI, F. L. BEYER, R.H.
HARRIS, MERIANI S. (2006). Delamination of organo-modified layered double
hydroxides in polyamide 6 by melt processing. POLYMER, vol. 47; p. 652-662,
ISSN: 0032-3861, doi: 10.1016/j.polymer.2005.11.080
S. SCHINTKE, M.STENGEL, L. COLOMBI CIACCHI, W-D.SCHNEIDER, M.SVETINA,
J.V.BARTH, O. SBAIZERO, S.ROITTI, MERIANI S., A. DE VITA (2004). Modeling
nucleation and insulating properties of oxide surfaces and ultrathin film. KEY
ENGINEERING MATERIALS, vol. 264; p. 485-489, ISSN: 1013-9826
S.PISCANEC, L.C.CIACCHI, E.VESSELLI, G.COMELLI, O.SBAIZERO, MERIANI S., A. DE
VITA (2004). Bioactivity of TiN-coated titanium implants. ACTA MATERIALIA, vol.
52; p. 1237-1245, ISSN: 1359-6454
A. COSSARO, D. CVETKO, G. BAVDEK, L. FLOREANO, R. GOTTER, A. MORGANTE, F.
EVANGELISTA, A. RUOCCO (2004). Copper-phthalocyanine induced reconstruction
of Au(110). JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER,
MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL, vol. 108; p. 14671, ISSN:
1520- 6106
A. COSSARO, L. FLOREANO, A. VERDINI, L. CASALIS, A. MORGANTE (2009).
Comment on "local methylthiolate adsorption geometry on au(111) from
photoemission core-level shifts". PHYSICAL REVIEW LETTERS, vol. 103; p. 119601,
ISSN: 0031-9007
A. COSSARO, R. MAZZARELLO, R. ROUSSEAU, L. CASALIS, A. VERDINI, A.
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562 MORGANTE
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563 MORGANTE
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564 MORGANTE
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A. MORGANTE (2009). A. Schiffrin, J. Reichert, Y. Pennec, W. Auwrter, A. WeberBargioni, M. Marschall, M. Dell'Angela, D. Cvetko, G. Bavdek, A. Cossaro, A.
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730 RAVALICO
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and insulating properties of oxide surfaces and ultra-thin films. KEY ENGINEERING
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ZULIANI F, SBAIZERO O., CHOUDHURY R, DE VITA A (2007). Dipole formation due
to ion exchange processes at the metal-ceramic Al/MgAl2O4 (001) interface. ACTA
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A. PORTA, L. MOIMAS, V. SERGO, SCHMID C. (2006). Devitrification phenomena of
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Surface characterization of bioglass fibers for biodegradabile polymer-ceramic
composite scaffolds. JOURNAL OF APPLIED BIOMATERIALS & BIOMECHANICS, vol.
4; p. 57, ISSN: 1722-6899
L. MARSICH, L. MOIMAS, V. SERGO, SCHMID C. (2009). Raman spectroscopic study
of bioactive silica-based glasses: The role of the alkali/alkali earth ratio on the
Non-Bridging Oxygen/BridgingOxygen (NBO/BO) ratio. SPECTROSCOPY (OTTAWA),
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L. MOIMAS, M. BIASOTTO, R. DI LENARDA, A. OLIVO, SCHMID C. (2006). Rabbit
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L. MOIMAS, V. SERGO, SCHMID C., E.PIRHONEN (2006). Bioactive porous scaffolds
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M. LAZZARINO, E. DE MARCHI, M. BRESSANUTTI, L. VACCARI, S.CABRINI, SCHMID
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M.BIASOTTO, F. ANTONIOLLI, N. SCUOR, V.RET, SCHMID C., R.DI LENARDA (2006).
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V. RET, M. BIASOTTO, F. ANTONIOLLI, C. SALVI, SCHMID C., E. DE STEFANO
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(2005). Structural and morphological investigation of ceria-promoted Al2O3
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791 SERGO Valter ABBASOVA, S. VISINTIN, SERGO V. (2005). Piezo-Spectroscopic Behavior of the
emission bands of alfa-alumina in the 13900-14250 cm-1 spectral range. JOURNAL
OF MATERIALS SCIENCE, vol. 40; p. 1593-1597, ISSN: 0022- 2461
792 SERGO Valter B. CODAN, T. GAIOTTO, R. DI NIRO, R. MARZARI, SERGO V. (2008). Implementation
of fibronectin patterning with a Raman spectroscopy microprobe for focal
adhesions studies in cells. In: Proceedings of International Congress of Biomedica
Engineering 2008. Singapore, 03/12/2008
793 SERGO Valter B. CODAN, T. GAIOTTO, R. DI NIRO, R. MARZARI, SERGO V. (2009). Patterning of
fibronectin using laser writer for force measurement in cells. In: Nanoscale
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794 SERGO Valter BONIFACIO A, FINAURINI S, KRAFFT C, PARAPINI S, TARAMELLI D, SERGO V. (2008).
Spatial distribution of heme species in erythrocytes infected with Plasmodium
falciparum by use of resonance Raman imaging and multivariate analysis.
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795 SERGO Valter C. KRAFFT, D. CODRICH, G. PELIZZO, SERGO V. (in stampa). Raman and FTIR
Imaging of lung tissue: bronchopulmonary sequestration. JOURNAL OF RAMAN
SPECTROSCOPY, ISSN: 0377-0486
796 SERGO Valter CODAN B, SERGO V. (2008). Implementation of maskless laser lithography using a
Raman spectroscopy microprobe. REVIEW OF SCIENTIFIC INSTRUMENTS, vol. 79,
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797 SERGO Valter CODAN B, VISINTIN S, DE MARIA L, SERGO V. (2006). A study on the effect of
micro and nano-debris impact on aerospace thermal barrier coating at high
temperature. In: Proceedings AIAA 57th International Astronautical Congress, IAC
2006. Valencia, Spain, October, 2-6 2006, NEW YORK: AIAA, vol. 8, p. 5652-5662,
ISBN/ISSN: 978-160560039-0
798 SERGO Valter DE MARIA L, RINALDI C, LUPETIN P, SERGO V. (2006). Portable optical system for
in-situ thermal barrier assessment of service operated blades. In: Proceedings of
the ASME Turbo Expo 2006. Barcellona, Spain, May, 6- 11, 2006, NEW YORK:
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799 SERGO Valter DONATI I, TRAVAN A, PELILLO C, SCARPA T, COSLOVI A, BONIFACIO A, SERGO V.,
PAOLETTI S (2009). Polyol Synthesis of Silver Nanoparticles: Mechanism of
Reduction by Alditol Bearing Polysaccharides. BIOMACROMOLECULES, vol. 2; p.
210-213, ISSN: 1525-7797
800 SERGO Valter FORNASIERO, P., SPEGHINI, A., DI MONTE, R., BETTINELLI, M., KASPAR, J.,
BIGOTTO, A., SERGO V., V., GRAZIANI, M. (2004). Laser excited luminescence of
trivalent lanthanide impurities and local structure in CeO2- ZrO2 mixed oxides.
CHEMISTRY OF MATERIALS, vol. 16; p. 1938-1944, ISSN: 0897-4756
801 SERGO Valter KRAFFT C, CODRICH D, PELIZZO G, SERGO V. (2008). Raman and FTIR Imaging of
lung tissue: congenital cystic adenomatoid malformation. ANALYST, vol. 133; p.
361-371, ISSN: 0003-2654, doi: 10.1039/B712958K
802 SERGO Valter KRAFFT C, CODRICH D, PELIZZO G, SERGO V. (2008). Raman and FTIR imaging of
lung tissue: methodology for control samples. VIBRATIONAL SPECTROSCOPY, vol.
46; p. 141-149, ISSN: 0924-2031, doi: 1016/j.vibspec.2007.12.007
803 SERGO Valter KRAFFT C, CODRICH D, PELIZZO G, SERGO V. (2008). Raman and FTIR microscopic
imaging of colon tissue: a comparative study. JOURNAL OF BIOPHOTONICS, vol. 1;
p. 154-169, ISSN: 1864-063X, doi: 1002/jbio.200710005
804 SERGO Valter KRAFFT C, CODRICH D, PELIZZO G, SERGO V. (2009). Raman and FTIR imaging of
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810 SERGO Valter
811 SERGO Valter
812 SERGO Valter
813 SERGO Valter
814 SERGO Valter
815 SERGO Valter
816 SERGO Valter
817 STANTA
Giorgio
818 STANTA
Giorgio
lung tissue: bronchopulmonary sequestration. JOURNAL OF RAMAN
SPECTROSCOPY, vol. 40; p. 595-603, ISSN: 0377-0486
KRAFFT C, SERGO V. (2006). Biomedical applications of Raman and infrared
spectroscopy to diagnose tissues. SPECTROSCOPY, vol. 20; p. 195-218, ISSN: 08876703
L. MARSICH, L. MOIMAS, SERGO V., C. SCHMID (2009). Raman spectroscopic study
of bioactive silica based glasses: The role of the alkali/alkali earth ratio on the nonbridging oxygen/bridging oxygen (NBO/BO) ratio. SPECTROSCOPY (OTTAWA), vol.
23; p. 227-232, ISSN: 0712-4813, doi: 10.3233/SPE-2009-0380
L. MOIMAS, G. DE ROSA, SERGO V., C. SCHMID (2006). "Bioactive porous scaffolds
for tissue engineering applications: investigation on the degradation process by
Raman spectroscopy and scanning electron microscopy". JOURNAL OF APPLIED
BIOMATERIALS & BIOMECHANICS, vol. 4; p. 102-109, ISSN: 1722-6899
L.DE MARIA, C.RINALDI, A.MARTINELLI, SERGO V. (2006). "Non-destructive
Integrity Assessment studies of TBC coatings". In: Proceedings of TURBINE FORUM
2006. Nizza, France, April, 26-28, 2006, vol. 1
MASCHIO S, ANEGGI E, TROVARELLI A, SERGO V. (2008). Influence of erbia or
europia doping on crystal structure and microsctructure of ceria-zirconia (CZ) solid
solutions. CERAMICS INTERNATIONAL, vol. 34; p. 1327-1333, ISSN: 0272-8842,
doi: 10.1016/j.ceramint.2007.03.006
N. SCUOR, P. GALLINA, H.V. PANCHAWAGH, R.L. MAHAJAN, O. SBAIZERO, SERGO
V. (2006). "Design of a novel MEMS platform for the biaxial stimulation of living
cells". BIOMEDICAL MICRODEVICES, vol. 87; p. 239-246, ISSN: 1387-2176
N.SCUOR, E.LUCCHINI, S. MASCHIO, S. LOCASTO, SERGO V. (2005). Wear
Mechanisms and residual stresses in alumina-based laminated cutting tools.
WEAR, vol. 258; p. 1372-1378, ISSN: 0043-1648
NAVARRA C.O, CADENARO M, ARMSTRONG S.R, JESSOP J, ANTONIOLLI F, SERGO
V., DI LENARDA R, BRESCHI L (2009). Degree of conversion of Filtek Silorane
Adhesive System and Clearfil SE Bond within the hybrid and adhesive layer: An in
situ Raman analysis. DENTAL MATERIALS, vol. 25; p. 1178-1185, ISSN: 0109-5641,
doi: 1016/j.dental.2009.05.009
NAVARRA C.O, CADENARO M, CODAN B, MAZZONI A, SERGO V., DE STEFANO
DORIGO E, BRESCHI L (2009). Degree of conversion and interfacial nanoleakage
expression of three one-step self-etch adhesives. EUROPEAN JOURNAL OF ORAL
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S. LO CASTO, E. LUCCHINI, O. SBAIZERO, SERGO V., N. SCUOR, M. NICOLICH (2005).
Characterization of Diamond-like coatings on Hard metal cutting tools via Raman
Spectroscopy and Piezo-spectroscopy. In: proceedings of 5th Int. Conference THE
coatings in manufactoring engineering, THESSALONIKI: Lab. for machine tools and
man. engineering, vol. 16, p. 375-383
SERGO V. (2004). Room temperature aging of Laminate Composites of Alumina/3mol%-Yttria-Stabilized Tetragonal Zirconia Polycrystals. JOURNAL OF THE
AMERICAN CERAMIC SOCIETY, vol. 87; p. 247-253, ISSN: 0002- 7820
TITILAYO A.K, UMERI A, SCUOR N, SERGO V. (2008). Raman investigation of the
Ageing of Ni-BaTiO3 Multilayer Ceramic Capacitors. JOURNAL OF MATERIALS
SCIENCE, vol. 43; p. 922-926, ISSN: 0022-2461, doi: 1007/s10853-007-2215-4
BONIN S, BRUNETTI D, BENDETTI E, GORJI N, STANTA G. (2006). Expression of
cyclin-dependent kinases and CDC25a phosphatase is related with recurrences
and survival in women with peri- and post-menopausal breast cancer. VIRCHOWS
ARCHIV, vol. 448; p. 539-544, ISSN: 0945-6317
BONIN S, BRUNETTI D, BENEDETTI E, DOTTI I, GORJI N, STANTA G. (2008).
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BOUIN'S FIXED TISSUES. JOURNAL OF CLINICAL PATHOLOGY, vol. 58; p. 313-316,
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BRUNETTI D, TAMARO P, FANIN R, CAVALIERI F, STANTA G. (2005). FAMILY
HISTORY OOF CANCER RISK OF SECOND MALIGNANCIES IN YOUNG CANCER
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GARDIOL D, ZACCHI A, PETRERA F, STANTA G., BANKS L (2006). Human disc large
and scrib are localized at the same regions in colon mucosa and changes in their
expression patterns are correlated with loss of tissues architecture during
malignant progression. INTERNATIONAL JOURNAL OF CANCER, vol. 119; p. 12851290, ISSN: 0020-7136
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DERMATOLOGY, vol. 115; p. 1108-1110, ISSN: 0007-0963
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ON LOW- AND HIGH-GRADE HUMAN BRAIN ASTROCYTOMAS. JOURNAL OF
PROTEOME RESEARCH, vol. 4(3); p. 698-708, ISSN: 1535-3893
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(2004). DETECTION OF SPIROCHETAL DNA SIMULTANEOUSLY IN SKIN BIOPSY,
PERIPHERAL BLOOD AND URINE FROM PATIENTS WITH ERITHEMA MIGRANS.
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POZZI MUCELLI S., ODREMAN F., GERARDI E., STANTA G., VINDIGNI A. (2006).
Proteomic studies on the white matter of human brain. JOURNAL OF
CHROMATOGRAPHY. B, vol. 833(1); p. 80-90, ISSN: 1570-0232
SCAGGIANE B, BONIN S, CRISTIANO L, SIRACUSANO S, STANTA G., DAPAS B,
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0735- 7907
SIRACUSANO S, NICCOLINI B, KNEZ R, TIBERIO A, BENEDETTI E, BONIN S.,
CICILIATO S, PAPPAGALLO GS, BELGRANO E, STANTA G. (2005). The simultaneous
use of telomerase, cytokeratin 20 and CD4 for bladder cancer detection.
EUROPEAN UROLOGY, vol. 47; p. 327-333, ISSN: 0302-2838
STANTA G. (2005). MORBID ANATOMY OF AGING. In: BALDUCCI L, LYMAN GW,
ERLSHER WB. COMPREHENSIVE GERIATRIC ONCOLOGY. AMSTERDAM: HARWOOD
ACADEMIC PUBLISHER
STANTA G., CESCATO A, BONIN S, BARBAZZA R (2008). BIOETHICS
CONSIDERATIONS FOR MEDICAL RESEARCH IN HUMAN ARCHIVE TISSUES: THE
POINT OF VIEW OF THE RESEARCHER. VIRCHOWS ARCHIV, vol. 453; p. 117- 119,
ISSN: 0945-6317
STANTA G., POZZI MUCELLI S, PETRERA F, BONIN S, BUSSOLATI G (2006). A NOVEL
FIXATIVE IMPROVES OPPORTUNITY OF NUCLEIC ACIDS AND PROTEOMIC ANALYSIS
IN HUMAN TISSUES. DIAGNOSTIC MOLECULAR PATHOLOGY, vol. 15; p. 115-123,
ISSN: 1052-9551
TOTHOVA SM., BONIN S., TREVISAN G., STANTA G. (2006). Mycosis fungoides: is it
a Borrelia burgdorferiassociated disease?. BRITISH JOURNAL OF CANCER, vol.
94(6); p. 879-883, ISSN: 0007-0920
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F.C. PEREIRA, E.P. BERGAMO, M.V. BOLDRIN ZANONI, L.M. MORETTO, P.UGO
(2006). Application of nanoelectrodes as sensors in analytical chemistry. QUIMICA
NOVA, vol. 29; p. 1054-1060, ISSN: 0100-4042
F.C. PEREIRA, L.M. MORETTO, M. DE LEO, M.V. BODRIN ZANONI, P.UGO (2006).
Gold nanoelectrode ensembles for direct trace electroanalysis of iodide.
ANALYTICA CHIMICA ACTA, vol. 575; p. 16-24, ISSN: 0003-2670, doi:
1016/j.aca.2006.05.056
F.C. PEREIRA, M.V. BOLDRIN ZANONI, L.M. MORETTO, P.UGO (2007). OPTICAL
AND MORPHOLOGICAL CHARACTERISTICS OF GOLD NANOSTRUCTURES. QUIMICA
NOVA, vol. 30; p. 1550-1554, ISSN: 0100-4042
F.C.PEREIRA, A.G.FOGG, P.UGO, E.P.BERGAMO, N.R.STRADIOTTO, M.V.B.ZANONI
(2005). Determination of Iodide and Idoxuridine at a Glutaraldehyde-Cross-Linked
Poly-L-Lysine Modified Glassy Carbon Electrode. ELECTROANALYSIS, vol. 17; p.
1309-1316, ISSN: 1040-0397, doi: 10.1002/elan.200403247
L.M. MORETTO, S. PANERO, B. SCROSATI, P.UGO (2009). Templated ensembles of
nanoelectrodes. In: YUEHE LIN, HARI SINGH NALWA. Handbook of electrochemical
nanotechnology. vol. 1, p. 87-105, STEVENSON RANCH: American Scientific
Publishers, ISBN/ISSN: 1-58883-121-3
L.M. MORETTO, T. KOHLS, A. CHOVIN, N. SOJIC, P.UGO (2008). Epifluorescence
imaging og electrochemically switchable Langmuir-Blodgett films of Nafion.
LANGMUIR, vol. 24; p. 6367-6374, ISSN: 0743-7463
L.M.MORETTO, N.PEPE, P.UGO (2004). Voltammetry of redox analytes at trace
concentrations with nanoelectrode ensembles. TALANTA, vol. 62; p. 1055-1060,
ISSN: 0039-9140
M. DE LEO, F.C. PEREIRA, L.M. MORETTO, P. SCOPECE, S. POLIZZI, P.UGO (2007).
Towards a better understanding of gold electroless deposition in track-etched
templates. CHEMISTRY OF MATERIALS, vol. 19; p. 5955-5964, ISSN: 0897-4756,
doi: 10.1021/cm071703j
M. DE LEO, L. M. MORETTO, O. BURIEZ, P.UGO (2009). ELECTROCHEMICAL
BEHAVIOR OF NANOELECTRODE ENSEMBLES IN THE IONIC LIQUID [BMIM][BF4].
ELECTROANALYSIS, vol. 21; p. 392-398, ISSN: 1040-0397, doi:
1002/elan.200804414
M.DE LEO, A. KUHN, P.UGO (2007). 3D-Ensembles of gold nanowires: preparation,
characterization and electroanalytical prospects. ELECTROANALYSIS, vol. 19; p.
227-236, ISSN: 1040-0397, doi: 1002/elan.200603724
M.ZAMUNER, D. TALAGA, F. DEISS, V. GUIEU, A. KUHN, P.UGO, N. SOJIC (2009).
Fabrication of a macroporous microwell array for surface-enhanced raman
scattering. ADVANCED FUNCTIONAL MATERIALS, vol. 19; p. 3129- 3135, ISSN:
1616-301X, doi: 10.1002/adfm.200900752
M.ZAMUNER, S. POZZI MUCELLI, M. TORMEN, G. STANTA, P.UGO (2008).
Electrochemical nanobiosensors and protein detection. EUROPEAN JOURNAL OF
NANOMEDICINE, vol. 1; p. 33-36, ISSN: 1662-5986, doi: 3884/0001.8
P.SCOPECE, L.A.BAKER, P.UGO, C.R.MARTIN (2006). Conical nanopore
membranes: solvent shaping of nanopores. NANOTECHNOLOGY, vol. 17; p. 1-6,
ISSN: 0957-4484, doi: 10.1088/0957-4484/17/15/057
P.SCOPECE, L.M.MORETTO, S.POLIZZI, P.UGO (2006). Composite films of poly(ester-sulphonated) and poly-(3- methylthiophene) for ion-exchange voltammetry
in acetonitrile solutions. ELECTROCHIMICA ACTA, vol. 51; p. 2153-2160, ISSN:
0013-4686, doi: 10.1016/j.electacta.2005.03.089
P.UGO (2006). Polymer based voltammetric sensors. In: C.A. GRIMES, E.C. DICKEY,
M.V. PISHKO. Encyclopedia of Sensors. vol. 8, p. 67-86, STEVENSON RANCH,
CALIFORNIA: American Scientific Publisher, ISBN/ISSN: 1-58883- 064-0
P.UGO, L.M. MORETTO (2007). Template deposition of metals. In: C. ZOSKI.
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HANDBOOK OF ELECTROCHEMISTRY. p. 678-709, AMSTERDAM: Elsevier,
ISBN/ISSN: ISBN-13: 978-0-444-51958-0
P.UGO, P. BERTONCELLO, F. VEZZA' (2004). Langmuir-Blodgett films of different
ionomeric polymers deposited on electrode surfaces. ELECTROCHIMICA ACTA, vol.
49; p. 3785-3793, ISSN: 0013-4686
S. POZZI MUCELLI, M. ZAMUNER, M. TORMEN, G. STANTA, P.UGO (2008).
Nanoelectrode ensembles as recognition platform for electrochemical
immunosensors. BIOSENSORS & BIOELECTRONICS, vol. 23; p. 1900-1903, ISSN:
0956-5663, doi: 10.1016/j.bios.2008.02.027 (2008)
Estratto della produzione scientifica del collegio dal 2004 al 2009 del
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A. Cossaro, L. Floreano, A. Verdini, L. Casalis, A. Morgante, Comment on "Local
Methylthiolate Adsorption Geometry on Au(111) from Photoemission Core-Level
Shifts'', Phys. Rev. Lett. 103 (11) 119601, 2009
Herrero, F. M.Toma, K.T. Al-Jamal, K. Kostarelos, A. Bianco, T. Da Ros, F. Bano, L.
Casalis, G. Scoles, M. Prato, "Synthesis and Characterization of a Carbon
Nanotube-Dendron Series for Efficient siRNA Delivery", J. Am. Chem. Soc. 131
(28), pp 9843-9848 (2009)
F. Bano, L. Fruk, B. Sanavio, M. Glettemberg, L. Casalis, C.M. Nemeyer, G. Scoles,
"Toward Multiprotein Nanoarrays Using Nanografting and DNA Directed
Immobilization of Proteins", NanoLetters 9(7) pp 2614-2618 (2009)
G. Cellot, E. Cilia, S. Cipollone, V. Rancic, A. Sucapane, S. Giordani, L. Gambazzi, H.
Markram, M. Grandolfo, D. Scaini, F. Gelain, L. Casalis, M. Prato, M. Giugliano, L.
Ballerini, "Carbon nanotubes direct interactions with neuronal membranes ignite
post spike excitability", Nature Nanotechnology, 4, pp 126 - 133 (2008)
M. Castronovo, S. Radovic, C. Grunwald, L. Casalis, M. Morgante, G. Scoles,
"Control of Steric Hindrance on Restriction Enzyme Reactions with Surface-Bound
DNA Nanostructures", NanoLetters, 8 (12), pp 4140-4145 (2008)
E. Mirmomtaz, M. Castronovo, C. Grunwald, F. Bano, D. Scaini, A.A. Ensafi, G.
Scoles, L. Casalis, "Quantitative Study of the Effect of Coverage on the
Hybridization Efficiency of Surface-Bound DNA Nanostructures", NanoLetters, 8
(12), pp 4134-4139 (2008)
A. Mazzarello, R. Rousseau, L. Casalis, A. Verdini, A. Kohlmeyer, L..Floreano, S.
Scandolo, A. Morgante, M.L..Klein, G. Scoles, "X-ray diffraction and computation
yield the structure of alkanethiols on gold(111)", Science 321 (5891), pp. 943-94
(2008)
D. Scaini, M. Castronovo, L. Casalis, G. Scoles, "Electron transfer mediating
properties of hydrocarbons as a function of chain length: A differential scanning
conductive tip atomic force microscopy investigation", ACS Nano 2 (3), pp. 507515
MORETTO, P.BERTONCELLO, F.VEZZA', P.UGO (2005). Electrochemistry of
cytochrome c incorporated in Langmuir-Blodgett films of Nafion and Eastman AQ
55. BIOELECTROCHEMISTRY, vol. 66; p. 29-34, ISSN: 1567- 5394, doi:
10.1016/j.bioelechem.2004.03.007
M. Pedio, B. Doyle, N. Mahne, A. Giglia, F. Borgatti, S. Nannarone, S.K.M. Henze, R.
Temirov, F.S. Tautz, L. Casalis, R. Hudej, M.F. Danisman and B.Nickel, "Growth of
pentacene on Ag(111) surface: a NEXAFS study", Applied Surface Science 254 (1),
pp. 103-107 (2007)
R. Mazzarello, A. Cossaro, A. Verdini, R. Rousseau, L. Casalis, M.F. Danisman, L.
Floreano, S. Scandolo, A. Morgante, G. Scoles, "Structure of a CH3S monolayer on
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Au(111) solved by the interplay between molecular dynamics calculations and
diffraction measurements", Physical Review Letters 98 (1), 016102 (2007)
M. Castronovo, F. Bano, S. Raugei, D. Scaini, M. Dell'Angela, R. Hudej, L.Casalis, G.
Scoles., "Mechanical stabilization effect of water on a membrane-like system",
Journal of the American Chemical Society 129 (9), pp. 2636-2641 (2007)
Danisman, L. Casalis, G. Scoles., " Supersonic molecular beam deposition of
pentacene thin films on two Ag(111) surfaces with different step densities",
Physical Review B - 72 (8), 085404, pp. 1-9 (2005)
Garza M.A., Moore C.G., Ahn Y.O., Bost J., Budai B., Canzian F., Chen K., Keku T.,
Lima CSP, Le Marchand L., Matsuo K., Plaschke J., Pufulete M., Thomas S.B., Toffoli
G., Wolf C.R., Little J., Taioli E. Meta and pooled analyses of the MTHFR C677T
polymorphism and colorectal cancer: a HuGSEC Review. Am. J. Epidemiol.
(Accepted)
Corona G., De Lorenzo E., Elia C., Simula M.P., Avellini C., Baccarani U., Lupo F.,
Tiribelli C., Colombatti A., Toffoli G. Differential protomi analysis of hepatocellular
carcinoma. Int. J. Oncol. (accepted, In press.)
De Re V., Cannizzaro R., Canzonieri V., Cecchin E., Caggiari L., De Mattia E., Pratesi
C., De Paoli P., Toffoli G. MTHFR polymorphisms on gastric cancer and on firstdegree relatives of patients with gastric cancer. Tumor Biology (accepted).
De Re V., Simula M.P., Cannizzaro R., Pavan A., De Zorzi M., Toffoli G., Canzonieri
V. Galectin-10, eosinophils, and celiac disease. Ann. N. Y. Acad. Sci.; 1173: 357364, 2009.
De Re V., Simula M.P., Pavan A., Garziera M., Marin D., Dolcetti R., De Vita S.,
Sansonno D., Geremia S., Toffoli G. Characterization of antibodies directed against
the immunoglobulin light kappa chain variable chain region (VK) of hepatitis C
virus-related type-II mixed cryoglobulinemia and B-cell proliferations. Ann. N.Y.
Acad. Sci.; 1173:152-160, 2009.
Toffoli G., Biason P., Russo A., De Mattia E., Cecchin E., Hattinger C.M., Pasello M.,
Alberghini M., Ferrari C., Scotlandi K., Picci P., Serra M. Effect of TP53 Arg72Pro
and MDM2 SNP309 polymorphisms on the risk of high-grade osteosarcoma
development and serviva. Clin. Cancer Res.; 15(10): 3550-3556, 2009.
Cecchin E., Innocenti F., D'Andrea M., Corona G., De Mattia E., Biason P.,
Buonadanna A., Toffoli G. Predictive role of the UGT1A1, UGT1A7 and UGT1A9
genetic variants and their hapltypes on the outcome of metastatic colorectal
cancer patients treated with fluorouracil, leucovorin, and irinotecan. J. Clin.
Oncol.; 27(15):2457-2465, 2009.
Visentin M., Simula M.P., Sartor F., Petrucco A., De Re V., Toffoli G. Identification
of proteins associated to multi-drug resistance in LoVo human colon cancer cells.
Int. J. Oncol.; 34(5): 1281-1289, 2009.
Simula M.P., Cannizzaro R., Marin M.D., Pavan A., Toffoli G., Canzonieri V., De Re
V. Two-dimensional gel proteome reference map of human small intestine.
Proteome Sci; 19;(7):10, 2009.
Corona G., Elia C., Casetta B., Crivellari D., Rosalen S., Bari M., Toffoli G. A liquid
chromatography-tandem mass spectrometry method for the simultaneous
determination of exemestane and its metabolite 17-dihydroexemestane in human
plasma. J. Mass Spectrom.; 44(6): 920-928, 2009.
De Mattia E., Toffoli G. C677T and A1298C MTHFR polymorphisms, a challenge for
antifolate and fluoropyrimidine-based therapy personalisation. Eur. J. Cancer;
45(8): 1333-1351, 2009.
Pavan A., Spina M., Canzonieri V., Sansonno S., Toffoli G., De Re V. Recent
prognostic factors in diffuse large B-Cell lymphoma indicate NF-KB pathway as a
target for new therapeutic strategies. Leuk. Lymphoma; 49(11):2048-2058, 2008.
Toffoli G., De Mattia E. Pharmacogenetic relevance of MTHFR polymorphisms.
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Pharmacogenomics; 9(9):1195-1206, 2008.
De Re V., Simula M.P., Cannizzaro R., Toffoli G. Studio di proteomica nell'infezione
da HCV: prospettive future per l'analisi delle risposte cliniche ai nuovi farmaci.
TUMORI ED INFEZIONI DA HIV. Linee guida nella terapia di combinazione
chemioterapia-HAART. GICAT. Editore: BIOMEDIA (Milano) 73- 82, 2008.
De Re V., De Vita S., Sansonno D., Toffoli G. Mixed cryoglobulinemia sindrome as
ad additional autoimmune disorder associated with risk for lymphoma
development. Blood; 111(12):5760, 2008.
Biason P., Masier S., Toffoli G. UGT1A1*28 and other UGT1A polymorphisms as
determinants of irinotecan toxicity. J Chemother; 20(2):158-165, 2008.
Corona G., Casetta B., Sandron S., Vaccher E., Toffoli G. Rapid and sensitive
analysis of vincristine in human plasma using on-line extraction combined with
liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass
Spectrom.; 22(4):519-525, 2008.
Corona G., Vaccher E., Sandron S., Sartor I., Tirelli U., Innocenti F., Toffoli G.
Lopinavir-Ritonavir Dramatically Affects the Pharmacokinetics of Irinotecan in HIV
Patients With Kaposi's Sarcoma. Clin. Pharmacol. Ther.; 83(4):601-606, 2008.
De Mattia E., Cecchin E., Toffoli G. Pharmacogenetics of stomach cancer therapy.
Tumori; 7(3): S66-S70, 2008. De Re V., Simula M.P., Cannizzaro R., Sansonno D.,
Canzonieri V., Gloghini A., Carbone A., Colombatti A., Marin M.D.,
De Zorzi M., Toffoli G. Hcv inhibits antigen processing and presentation and
induces oxidative stress response in gastric mucosa.. Proteomics Clin. Appl.;
2:1290-1299, 2008.
Baldo P., Bearz A., Cannizzaro R., Canzonieri V., Dal Maso L., Di Lauro V., Lazzarini
R., Milan I., Rupolo M., Scalone S., Spazzapan S., Toffoli G., Truccolo I., Carbone A.
Interferon-alpha for follicular lymphoma [Protocol], Cochrane Database of
Systematic Reviews, 2009.
De Re V., Caggiari L., Dolcetti R., De Zorzi M., Simula M. P., Toffoli G. Tumori
indotti da agenti infettivi: Immunogenetica delle linfoproliferazioni HCV-associate:
Il ruolo dell'HLA di classe II Biomedia, 2007.
De Re V., Sansonno D., De Paoli P., Geremia S., Gatti G., Caggiari L., Simula M. P.,
Toffoli G. Recent patents relating to HCV molecules like putative targets for
therapeutic intervention. Recent Patents on DNA & Gene Sequences; 1: 186-194,
2007.
Toffoli G., Cecchin E. Pharmacogenetics and stomach cancer: an update.
Pharmacogenomics; 8(5):497-505, 2007.
Toffoli G, De Mattia E, Cecchin E, Biason P., Masier S., Corona G. Pharmacology of
epidermal growth factor inhibitors. Int. J. Biol. Markers; 22 (1 Suppl 4):S24-S39,
2007.
Toffoli G, Cecchin E. Clinical implications of genetic polymorphisms on stomach
cancer drug therapy. Pharmacogenomics J.; 7(2): 76-80, 2007.
Toffoli G, Cecchin E, Corona G, Russo A, Buonadonna A, D'Andrea M, Pasetto LM,
Pessa S, Errante D, De Pangher V, Giusto M, Medici M, Gaion F, Sandri P, Galligioni
E, Bonura S, Boccalon M, Biason P, Frustaci S. The role of UGT1A1*28
polymorphism in the pharmacodynamics and pharmacokinetics of irinotecan in
patients with metastatic colorectal cancer. J. Clin. Oncol.; 24(19):3061-3068, 2006.
Toffoli G, Innocenti F. MTHFR and ALL risk: a challenge. Leuk Lymphoma;
47(7):1203-1204, 2006.
Corona G and Toffoli G. Interazioni farmacocinetiche tra inibitori delle protease e
farmaci antiblastici: influenza di lopinavir-ritonvair sulla farmacocinetica
dell'Irinotecan. Tumori ed Infezioni da HIV, patogenesi e studi clinici del GICAT;
75-84; Ed Biomedia, 2006.
Cecchin E, Corona G and Toffoli G. Reply to the letter to the editor from chowbay
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et al. Clin. Cancer Res.; 12(6):1942, 2006.
Gasparini G, Longo R, Torino F, Gattuso D, Morabito A and Toffoli G. Is tailored
therapy feasible in oncology? Crit. Rev. Oncol. Hematol.; 57(1):79-101, 2006.
Stocco G, Martelossi S, Sartor F, Toffoli G, Lionetti P, Barabino A, Fontana M,
Decorti G, Bartoli F, Giraldi T, Ventura A. Prevalence of methylenetetrahydrofolate
reductase polymorphisms in young patients with inflammatory bowel disease.
Dig. Dis. Sci.; 51(3):474-479, 2006.
Crivellari D, Lombardi D, Corona G, Massacesi C, Talamini R, Sorio R, Magri MD,
Lestuzzi C, Lucenti A, Veronesi A, Toffoli G. Innovative schedule of oral idarubicin
in elderly patients with metastatic breast cancer: comprehensive results of a
phase II multi-institutional study with pharmacokinetic drug monitoring. Ann.
Oncol.;17:807-812,2006.
Sorio R, Toffoli G, Crivellari D, Bearz A, Corona G, Colussi AM, Libra M, Talamini R,
Veronesi A. Oral etoposide in elderly patients with advanced non small cell lung
cancer: a clinical and pharmacological study. J Chemother; 18(2):188-191, 2006.
Toffoli G, Masier S, Biason P, Cecchin E, Corona G. Pharmacogenetics in the
personalisation of Docetaxel based chemotherapy. Tumori; 5(1):S9, 2006.
Biason P, Toffoli G. Sarcomas and pharmacogenetics. Pharmacogenomics J.;
6(6):585-601, 2005.
Cecchin E, Corona G, Masier S, Biason P, Cattarossi G, Frustaci S, Buonadonna A,
Colussi A and Toffoli G. Carboxylesterase isoform 2 mRNA expression in peripheral
blood mononuclear cells is a predictive marker of the irinotecan to SN38
activation step in colorectal cancer patients. Clin. Cancer Res.; 11(19 Pt 1):69016907, 2005.
Corona G, Vaccher E, Cattarossi G, Sartor I and Toffoli G. Potential hazard of
pharmacokinetic interactions between lopinavir-ritonavir protease inhibitors and
irinotecan. AIDS; 19(17):2043-2044, 2005.
Gasparini G, Longo R, Torino F, Barone C and Toffoli G. Tailored therapy of
colorectal cancer: results, challenges and future directions. Cancer J.; 11(3):175188, 2005.
Toffoli G, Cecchin E. Pharmacogenetics in ovarian cancers. Bardos, A.P.(ed) in
Treatment of Ovarian Cancer (Horizons in Cancer Research), Frank Columbus Nova
Science Publishers; pag 1-25, 2005.
Cecchin E, Russo A, Campagnutta E, Martella L and Toffoli G. Lack of association of
CYP1 B1*3 polymorphism and ovarian cancer in a caucasian population. Int. J.
Biol. Markers; 19:160-163, 2004.
Cecchin E, Russo A, Corona G, Campagnutta E, Martella L, Boiocchi M and Toffoli
G. UGT1A1*28 polymorphism in ovarian cancer patients. Oncol. Rep.; 12:457-462,
2004.
Crivellari D, Lombardi D, Spazzapan S, Veronesi A and Toffoli G. New oral drugs in
older patients: a review of idarubicin in elderly patients. Crit. Rev. Oncol.
Hematol.; 49:153-163, 2004.
Libra M, Navolanic PM, Talamini R, Cecchin E, Sartor F, Tumolo S, Masier S, Travali
S, Boiocchi M and Toffoli G. Thymidylate synthetase mRNA levels are increased in
liver metastases of colorectal cancer patients resistant to fluoropyrimidine-based
chemotherapy. BMC Cancer; 4:11, 2004.
Toffoli G, Sorio R, Basso B, Aita P, Corona G, Ruolo G, Boiocchi M. Pharmacokinetic
comparison of 120-hour infusion versus hyperfractionated oral administration of
idarubicin. J Chemother; 16:193-200, 2004.
Toffoli G, Corona G, Basso B, Boiocchi M. Pharmacokinetic optimisation of
treatment with oral etoposide. Clin. Pharmacokinet.; 43:441-466, 2004.
Toffoli G, Corona G, Cattarossi G, Boiocchi M, Di Gennaro G, Tirelli U, Vaccher E.
Effect of highly active antiretroviral therapy (HAART) on pharmacokinetics and
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pharmacodynamics of doxorubicin in patients with HIV-associated non- Hodgkin's
lymphoma. Ann. Oncol; 15:1805-1809, 2004.
Toffoli G, Cecchin E. Pharmacogenomics and stomach cancer. Pharmacogenomics;
5:627-641, 2004.
Corona G, Toffoli G. High Throughput Screening of Genetic Polymorphisms by
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.
Comb. Chem. High Troughput Screen.; 7:707-725, 2004.
Toffoli G. Ruoli differenti per la professione di ricercatore biomedico: Il ruolo del
farmacologo. Tumori; 6:S16-S17, 2004.
Baldo P, Bearz A, Cannizzaro R, Canzonieri V, Dal Maso L, Di Lauro V, Lazzarini R,
Milan I, Rupolo M, Scalone S, Spazzapan S, Toffoli G, Truccolo I, Carbone A for the
working group of the "Centro di Riferimento Oncologico" Aviano - Italy.
Interferon-alpha for follicular lymphoma. (Protocol) The Cochrane Database of
Systematic Reviews 2004, Issue 1. Art. No.: CD004629. DOI:
10.1002/14651858.CD004629.
Toffoli G, Cecchin E. Uridine Diphosphoglucuronosyl Transferase and
Methylenetetrahydrofolate Reductase Polymorfisms as genomic predictors of
toxicity and response to Irinotecan-, Antifolate- and Fluoropyrimidine-Based
Chemotherapy. J Chemother; 16(4):31-35, 2004.
G. Ambrosetti, N. Johner, C. Grimaldi, T. Maeder, P. Ryser and A. Danani ,
"Electron Tunneling in conductor-insulator composites with spherical fillers",
Journal of Applied Physics, 106, 016103 (2009)
Pavan G.M., Danani A., Pricl S., Smith D.K., "Modeling the Multivalent Recognition
between Dendritic Molecules and DNA: Understanding How Ligand Sacrifice and
Screening Can Enhance Binding", Journal of the American Chemical Society 2009,
131(28):9686-94
Posocco P., Pavan G. M., Scocchi G., Handgraaf J.-W., Malek A., Maly M.,
Fermeglia M., Fraaije H.J.G.E.M., Catapano C., Danani A., Pricl S., "Base Invaders.
Coupling Experiments and Multiscale Modeling of Dendrimer-Based siRNA
Delivery Agents", Advances in Science and Technology, (2008), 57, 154-159.
G. Ambrosetti, N. Johner, C. Grimaldi, A. Danani, P. Ryser ,"Percolative Properties
of Hard Oblate Ellipsoids of Revolution With a Soft Shell", Phys. Rev. E 78, 061126
(2008)
G. Scocchi, P. Posocco, A. Danani, S. Pricl, M. Fermeglia, "To the nanoscale, and
beyond! Multiscale molecular modelling of polymer-clay nanocomposites", Fluid
Phase Equilibria 261 (1-2), 366 , (2007).
G. E. Tommei, F. Baletto, R. Ferrando, R. Spadacini, and A. Danani, Phys. Rev. B 69,
(2004) 115426 R. Trotta, A. Polimeni, M. Capizzi, F. Martelli, S. Rubini, M.
Francardi, A. Gerardino, and L. Mariucci Light polarization control in strainengineered GaAsN/GaAsN:H heterostructures Appl. Phys. Lett. 94, 261905 (2009)
G. Ciatto, F. Boscherini, A. Amore Bonapasta, F. Filippone, A. Polimeni, M. Capizzi,
M. Berti, G. Bisognin, D. De Salvador, L. Floreano, F. Martelli, S. Rubini, L.
Grenouillet, Local structure of nitrogen-hydrogen complexes in dilute nitrides
Phys. Rev. B79, 165205 (2009)
Jabeen F., Rubini S., Martelli F., Growth of III-V semiconductor nanowires by
molecular beam epitaxy Microelectronics Journal 40, 442 (2009).
Jabeen F., Rubini S., Grillo V., Felisari L., Martelli F., Room temperature
luminescent InGaAs/GaAs core-shell nanowires Appl. Phys. Lett. 93, 083117
(2008)
Felisari L., Grillo V., Martelli F., Trotta R., Polimeni A., Capizzi M., Jabeen F.,
Mariucci L., In-plane band gap modulation investigated by secondary electron
imaging of GaAsN/GaAsN:H heterostructures Appl. Phys. Lett. 93, 102116 (2008)
Trotta R., Polimeni A., Capizzi M., Giubertoni D., Bersani M., Bisognin G., Berti M.,
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Rubini S., Martelli F., Mariucci L., Francardi M., Gerardino A., Effect of hydrogen
incorporation temperature in in plane-engineered GaAsN/GaAsN:H
heterostructures Appl. Phys. Lett. 92, 221901 (2008)
Jabeen F., Grillo V., Rubini S., Martelli F., Self-catalyzed growth of GaAs nanowires
on Si by molecular beam epitaxy Nanotechnology 19 , 275711 (2008)
Bisognin G., De Salvador D., Napolitani E., Berti M., Polimeni A., Capizzi M., Rubini
S., Martelli F., Franciosi A., Highresolution X-ray diffraction in situ study of very
small complexes: The case of hydrogenated dilute nitrides Journal of Applied
Crystallography 41 , 366 (2008)
Polimeni A., Pettinari G., Trotta R., Masia F., Felici M., Capizzi M., Lindsay A.,
O'Reilly E. P., Niebling T., Stolz W., Klar P. J., Martelli F., Rubini S.,
Photoluminescence under magnetic field and hydrostatic pressure for probing the
electronic properties of GaAsN Physica Status Solidi (A) 205, 107 (2008)
Kleekajai S., Jiang F., Colon K., Stavola M., Fowler W. B., Martin K. , R., Polimeni A.,
Capizzi M., Hong Y. G.,. Xin H. P, Tu C. W., Bais G., Rubini S., Martelli F., Vibrational
properties of the H-N-H complex in dilute III-N-V alloys: Infrared spectroscopy and
density functional theory Physical Review B77 , 085213 (2008)
Berti M., Bisognin G., De Salvador D., Napolitani E., Vangelista S., Polimeni
A.,Capizzi M., Boscherini F., Ciatto G., Rubini S., Martelli F., Franciosi A., Formation
and dissolution of D-N complexes in dilute nitrides Phys. Rev. B 76, 205323 (2007)
Bisognin G., De Salvador D., Napolitani E., Berti M., Polimeni A., Felici M., Capizzi
M., Bais G., Jabeen F., Piccin M., Rubini S., Martelli F., Franciosi A., Thermal
evolution of small N-D complexes in deuterated dilute nitrides revealed by insitu
high resolution X-ray diffraction phys. stat. sol. (a) 204, 2766 (2007)
Martelli F., Piccin M.,. Bais G, Jabeen F., Ambrosini S., Rubini S., Franciosi A.,
Photoluminescence of Mn-catalyzed GaAs nanowires grown by molecular beam
epitaxy Nanotechnology, 18, 125603 (2007).
Geddo M., Ciabattoni T., Guizzetti G., Galli M., Patrini M., Polimeni A., Trotta R.,
Capizzi M., Bais G., Piccin M., Rubini S., Martelli, F., Franciosi A., Photoreflectance
and Reflectance investigation of deuterium irradiated GaAsN, Appl. Phys. Lett. 90,
091907 (2007).
Rubini S., Piccin M., Bais G., Jabeen F., Martelli F., Franciosi A., GaAs nanowires by
Mn-catalysed molecular beam epitaxy Journal of Physics: Conference Series 61,
992 (2007)
Piccin M., Bais G., Grillo V., Jabeen F., De Franceschi S., Carlino E., Lazzarino M.,
Romanato F., Businaro L., Rubini S. , Martelli F., Franciosi A., Growth by molecular
beam epitaxy and electrical characterization of GaAs nanowires Physica E 37, 134
(2007).
Masia F., Pettinari G., Polimeni A., Felici M., Miriametro A., Capizzi M., Lindsay A.,
Healy S. B., O'Reilly E. P., Cristofoli A., Bais G., Piccin M., Rubini S., Martelli F.,
Franciosi A., Klar P. J., Volz K., and Stolz W., Interaction between conduction band
edge and nitrogen states probed by carrier effective mass measurements in
GaAs1-xNx Phys. Rev. B73, 077201 (2006).
Felici M., Polimeni A., Salviati G., Lazzarini L., Armani N., Masia F., Capizzi M.,
Martelli F., Lazzarino M., Bais G., Piccin M., Rubini S., Franciosi A. ,In-plane band
gap engineering by modulated hydrogenation of dilute nitride semiconductors
Adv. Mater. 18, 1993 (2006).
Martelli F., Rubini S., Piccin M., Bais G., Jabeen F., De Franceschi S., Grillo V.,
Carlino E., D'Acapito F., Boscherini F., Cabrini S., Lazzarino M., Businaro L.,
Romanato F., Franciosi A., Mn induced growth of GaAs nanowires, Nano Letters,
6, 2130 (2006).
Frassanito M.C., De Giorgi M., Rinaldi R., Cingolani R., Rubini S., Piccin M.,
Cristofoli A., Bais G., Martelli F., Carlino E., Franciosi A., Microphotoluminescence
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characterization of alloy fluctuations in InGaAsN/GaAs quantum wells emitting at
1.3?m Semic. Sci. Technol. 21, 1207 (2006)
Bisognin G., De Salvador D., Drigo A.V., Napolitani E., Sambo A., Berti M., Polimeni
A., Felici M., Capizzi M., Güngerich M., Klar P.J., Bais G., Jabeen F., Piccin M.,
Rubini S., Martelli F., Franciosi A., Hydrogen-nitrogen complexes in dilute nitride
alloys: origin of the compressive lattice strain Appl. Phys. Lett. 89, 061904 (2006).
Carlino E., Martelli F., Rubini S., Franciosi A., Catalyst incorporation in ZnSe
nanowires Phil. Mag. Lett. 86, 261 (2006). Rubini S., Bais G., Cristofoli A., Piccin M.,
Duca R., Nacci C., Modesti S., Carlino E. , Martelli F. Franciosi A., Nitrogen induced
hindering of In incorporation in InGaAsN Appl. Phys. Lett. 88, 141923 (2006).
Pelucchi E., Kumar D., Lazzarino M., Rubini S., Franciosi A., Controlling interface
reactivity and Schottky barrier height in Au/ZnSe (001) junctions, J. Vac. Sci.
Technol. B24, 1259 (2006).
D'Acapito F., Smolentsev G., Boscherini F., Piccin M., Bais G., Rubini S., Martelli F.,
Franciosi A., Site of Mn in d-doped GaAs: X-ray absorption spectroscopy Phys. Rev.
B73, 035314 (2006)
Duca R., Ceballos G., Nacci C., Furlanetto D., Finetti P., Modesti S., Cristofoli A.,
Bais G., Piccin M., Rubini S., Martelli F., Franciosi A., In-N and N-N correlation in
InxGa1-xAs1-Ny/GaAs quasi-lattice matched quantum wells: A cross sectional
scanning tunnelling microscopy study Phys. Rev. B72, 075311 (2005)
Bais G., Cristofoli A., Jabeen F., Piccin M., Carlino E., Rubini S., Martelli F., Franciosi
A., InAsN/GaAs(N) quantum-dot and InGaNAs/GaAs quantum-well emitters: A
comparison Appl. Phys. Lett 86, 233107 (2005)
Colli A., Hoffman S., Ferrari A.C., Ducati C., Martelli F., Rubini S., Franciosi A. ,
Robertson J., Low temperature synthesis of ZnSe nanowires and nanosaws by
catalyst assisted molecular beam epitaxy Appl. Phys. Lett 86, 153103 (2005)
Colli A., Hoffman S., Ferrari A.C., Martelli F., Rubini S., Ducati C., Franciosi A.,
Robertson J., Selective growth of ZnSe and ZnCdSe nanowires by molecular beam
epitaxy Nanotecnology 16, S139 (2005).
Modesti S., Duca R., Finetti P., Ceballos G., Piccin M., Rubini S., Franciosi A.,
Microscopic Mechanisms of Self- Compensation in Si ?-doped GaAs Phys. Rev. Lett
92, 086104-1 (2004)
Pelucchi E., Rubini S., Bonanni B., Franciosi A., Zaui A., Peressi M., Baldereschi A.,
De Salvador D., Berti M., Drigo A., Romanato F., Structural and electronic
properties of wide band gap Zn1-xMgxSe alloys J. Appl. Phys. 95, 4184 (2004)
Roveda L, Zonta A, Staffieri F, Timurian D, DiVenere B, Bakeine GJ, Crovace A, Prati
U., Experimental modified orthotopic piggy-back liver autotransplantation, Appl
Radiat Isot. 2009 Jul;67(7-8 Suppl):S306-8. Epub 2009 Mar 27.
Zonta A, Pinelli T, Prati U, Roveda L, Ferrari C, Clerici AM, Zonta C, Mazzini G,
Dionigi P, Altieri S, Bortolussi S, Bruschi P, Fossati F., Extra-corporeal liver BNCT for
the treatment of diffuse metastases: what was learned and what is still to be
learned, Appl Radiat Isot. 2009 Jul;67(7-8 Suppl):S67-75. Epub 2009 Mar 28.
Altieri S, Bortolussi S, Bruschi P, Chiari P, Fossati F, Stella S, Prati U, Roveda L,
Zonta A, Zonta C, Ferrari C, Clerici A, Nano R, Pinelli T., Neutron autoradiography
imaging of selective boron uptake in human metastatic tumours, Appl Radiat Isot.
2008 Dec;66(12):1850-5. Epub 2008 May 29.
Asteria CR, Gagliardi G, Pucciarelli S, Romano G, Infantino A, La Torre F, Tonelli F,
Martin F, Pulica C, Ripetti V, Diana G, Amicucci G, Carlini M, Sommariva A,
Vinciguerra G, Poddie DB, Amato A, Bassi R, Galleano R, Veronese E, Mancini S,
Pescio G, Occelli GL, Bracchitta S, Castagnola M, Pontillo T, Cimmino G, Prati U,
Vincenti R., Anastomotic leaks after anterior resection for mid and low rectal
cancer: survey of the Italian Society of Colorectal Surgery., Tech Coloproctol. 2008
Jun;12(2):103-10. Epub 2008 Jun 10.
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972
Bakeine GJ, Bertolotti A, Latina M, Congiu T, Prati U, Roveda L, Trotta F, Tormen
M, Fabrizio ED, Carlini G, Facoetti A, Nano R., Surface properties and implantation
site affect the capsular fibrotic overgrowth, J Biomed Mater Res A. 2007 Dec
15;83(4):965-9.
Meka, H., Werner, F. Cordell, S., Onesti, S. & Brick, P. (2005). Crystal structure and
RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II. Nucleic Acid
Res. 33, 6435-6444.
Blanquet, S., Plateau, P. & Onesti, S. (2005) "Class II aminoacyl-tRNA synthetases:
lysyl-tRNA synthetase" in Aminoacyl-tRNA synthetases (Ed M. Ibba, C. Francklyn
and S. Cusack; Landes Biosciences).
Paraskevopoulou, C., Fairhurst, S.A., Lowe, D.J., Brick, P. & Onesti, S. (2006). The
Elongator subunit Elp3 contains a Fe4S4 cluster and binds S-adenosylmethionine.
Mol. Microbiol. 59, 795-806.
Palmieri, G., Casbarra, A., Fiume, I., Catara, G., Capasso, A., Marino, G., Onesti, S.
& Rossi, M. (2006) Identification of the first archaeal oligopeptide binding protein
from the hyperthermophile Aereopyrum pernix. Extremophiles, 10, 393- 402.
Costa A., Pape T., van Heel M., Brick P., Patwardhan A. & Onesti S. (2006a)
Structural studies of the archaeal MCM complex in different functional states. J.
Struct. Biol., 156, 210-219.
Costa A., Pape T., van Heel M., Brick P., Patwardhan A. & Onesti S. (2006b).
Structural basis of the Methanobacter thermautotrophicus MCM helicase activity.
Nucleic Acid Res. 34, 5829-5838.
Vinaya Sampath V., Bindu Balakrishnan B., Verma-Gaur J., Onesti S. & Sadhale P.P.
(2008) Unstructured N terminus of Rpb4 contributes to the interaction of
Rpb4/Rpb7 subcomplex with the core RNA polymerase II in S. cerevisiae. J. Biol.
Chem., 283, 3923-3931.
Costa A & Onesti, S. (2008). The MCM complex: (just) a replicative helicase?
Biochem. Trans. 36, 136-140.
Costa A., van Dujnen G., Medagli B., Chong J., Sakakibara N., Kelman Z., Nair S.K.,
Patwardhan A. & Onesti S. (2008). Cryo-electron microscopy reveals a novel DNA
binding site on the MCM helicase. EMBO J., 27, 2250-2258.
Bae B., Chen Y.-H., Costa A., Onesti S., Brunzelle J.S., Lin Y., Cann I.K.O. & Nair S.K.
(2009) Crystal Structure of an Archaeal MCM Homolog Provides Insights into the
Architecture of the Replicative Helicase. Structure 17, 211-222.
Jenkinson, E.R., Costa A., Leech, A.P., Patwardhan A., Onesti S. & Chong, J.P
(2009). Mutations in sub-domain B of the MCM helicase affect DNA binding and
modulate conformational transitions. J. Biol. Chem. 284, 5654-5661.
Costa, A & Onesti, S. (2009). Structural biology of MCM helicases. Crit. Rev.
Biochem. Mol. Biol. 44, 326-342.
G. Mori, M. Lazzarino, D. Ercolani, G. Biasiol, L. Sorba, Magnetic field and
Temperature dependance of an atomic force microscope-defined quantum point
contact, J. Vac. Sci. //Technol/. B 22, 570 (2004)
M. Lazzarino, M. Padovani, G. Mori, L. Sorba, M. Fanetti and M. Sancrotti,
Chemical composition of GaAs oxides grown by local anodic oxidation: a spatially
resolved Auger study., Chem. Phys. Lett/. 402, 155, (2005)
D. Ercolani, M. Lazzarino, G. Mori, B. Ressel, L. Sorba, A. Locatelli, S. Cherifi, A.
Ballestrazzi and S. Heun, Gas Oxide Desorption under Extreme Ultraviolet Photon
Flux. Advanced Functional Materials, *15* (2005) 587.
G. Mori, M. Lazzarino, D. Ercolani, L. Sorba, S. Heun and A. Locatelli, Desorption
dynamics of oxide nanostructures fabricated by local anodic oxidation. J. Appl.
Phys/. 97 art.n. 115324 (2005)
G. Mori, M. Lazzarino D. Ercolani, G. Biasiol, A. Locatelli, L. Sorba and S. Heun, Xray induced variation of the chemistry of GaAs/AlAs oxide nanostructures. Nucl.
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989
Instr. and Meth. In Phys. Res. B, 246, (2005)
M. Lazzarino, E. De Marchi, M. Bressanutti, L. Vaccari, S. Cabrini, C. Schmid, R.
Poetes R and G. Scoles, Twin cantilevers with a nanogap for single molecule
experimentation. Microelectronic Eng., 83, 1309 (2006)
E. Pelucchi, D. Kumar, M. Lazzarino, S. Rubini and A. Franciosi, Controlling
interface reactivity and Schottky barrier height in Au/ZnSe(001) junctions. J. Vac.
Sci. Technol/ B 24, 1259 (2006)
M. Felici, A. Polimeri, G. Salviati, L. Lazzaroni, N. Armani, F. Masia, M. Capizzi, F.
Martelli, M. Lazzarino, G. Bais, M. Piccin, S. Rubini and A. Franciosi, In-plane
bandgap engineering by modulated hydrogenation of dilute nitride
semiconductors. Adv. Mat., 18, 1993 (2006)
F. Martelli, S. Rubini,M. Piccin, G. Bais, F. Jabeen, S. Defranceschi, V. Grillo, E.
Carlino, F. D'Acapito, F. Boscherini, S. Cabrini, M. Lazzarino, L. Businaro, F.
Romanato, and A. Franciosi, Manganese-induced growth of GaAs nanowires.
Nanoletters, 6, 2130 (2006)
M. Lazzarino, G. Mori, L. Sorba, D. Ercolani, G. Biasiol, S. Heun and A. Locatelli,
Chemistry and formation process of Ga (Al)As oxide during local anodic oxidation
nanolithography, Surf. Sci., 600, 3739 (2006)
M. Piccin, G. Bais, V. Grillo, F. Jabeen, S. De Franceschi, E. Carlino, M. Lazzarino, F.
Romanato, L. Businaro, S. Rubini, F. Martelli and A. Franciosi, Growth by molecular
beam epitaxy and electrical characterization of GaAs nanowires. Physica E
doi.org/10.1016/j.physe.2006.07.002
A. Qazi, D. Nonis, A. Pozzato, M. Tormen, M. Lazzarino, S. Carrato and G. Scoles,
Symmetric twin cantilever for single molecole detection. Appl. Phys. Lett, *90*,
173118 (2007)
Maione, IA; Macucci, M; Iannaccone, G; Basso, G; Pellegrini, B; Lazzarino, M;
Sorba, L; Beltram, F. , Probing Pauli blocking with shot noise in resonant tunneling
diodes: Experiment and theory. Phys. Rev. B, 75, 125327 (2007)
V Toffoli, F Esch, M Melli, F Cataruzza, A Pozzato, S Carrato, G Scoles, M Tormen
and M Lazzarino, Intrinsically aligned chemo-mechanical functionalization of twin
cantilever structure., Nanotechnology, 19 445502 (2008)
Kondra, S., Laishram, J., Ban, J., Migliorini, E., Di Foggia, V., Lazzarino, M., Torre, V.,
Ruaro, M.E., Integration of confocal and atomic force microscopy images, Journal
of Neuroscience, Methods, 177. 94 (2009)
V Toffoli, F Esch, M Melli, A Pozzato, M Tormen and M Lazzarino, Chemical
functionalization of atomically flat cantilever surfaces, Microelectronic Eng., 86,
1200 (2009)
M.Polano, A. Bek, F. benetti, M. Lazzarino and G. Legname, Structural Insights into
Alternate Aggregated Prion Protein Forms, J. Mol. Biol, 393, 1033 (2009)
A morphological analysis of growth cones of DRG neurons combining Atomic
Force and Confocal J. Laishram, S. Kondra, D. Avossa, E. Migliorini, M. Lazzarino
and V. Torre, Microscopy. Atomic Force and Confocal Microscopy. J. Struct. Biol.,
168, 366 (2009)
D. Cojoc, "Optical manipulation in support of high spatial and temporal resolution
experiments in Biophysics", Invited Review, SPIE Reviews, Fall 2009.
E. Ferrari, L. Selvaggi, A. Moradi, S. Cantucci, P. Beuzer and D. Cojoc, "Optimized
double-view imaging improves observation of non-spherical particles in optical
tweezers microscopy", J. of Optics: Pure and Applied Optics, (submitted 2009).
D. Cojoc, H. Amenitsch, E. Ferrari, M. Rappolt, B. Marmirolli, B. Sartori, G. Grenci,
S. Santucci, M., Burghammer and Christian Riekel "Synchronized optical trapping
and scanning micro-focused X-ray diffraction", Nat. Methods (submitted 2009).
V. Garbin, B. Dollet, M. Overvelde, D. Cojoc, E. Di Fabrizio, L. Van Wijngaarden, A.
Prosperetti, N. de Jong, D. Lohse, and M. Versluis "History force on coated
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microbubbles propelled by ultrasound" , Physics of Fluids (accepted July 2009).
D. Cojoc, H. Amenitsch, C. Riekel, M. Rappolt, M. Burghammer, S. Santucci, G.
Grenci, B. Sartori, B. Marmiroli, "X-ray microdiffraction of microsamples
manipulated in fluidic environment without mechanical contact", ESRF Highlights
2008 48-49 http://www.esrf.eu/files/Highlights/HL2008.pdf .(2009)
V. Garbin, G. Volpe, E. Ferrari, M. Versluis, D. Cojoc and D. Petrov, "Mie scattering
distinguishes the topological charge of an optical vortex: a homage to Gustav
Mie", New Journal of Physics, 11, 013046, (2009).
E. Connell, A. Giniatullina, J.L.K. Him, P. Scott, E. Ferrari, A. Roseman, D. Cojoc, A.R.
Brisson and B. Davletov, "Crosslinking of Phospholipid Membranes is a Conserved
Property of Calcium-Sensitive, Synaptotagmins", J. Mol. Biol. 380 42 (2008).
M. Mihailescu, A. Preda, D. Cojoc, E. Scarlat, L. Preda, "Diffractive patterns from a
phyllotaxis type arrangement", Opt. Laser Eng. 46, 802-809, (2008).
J. Garcia, V. Mico, D. Cojoc and Z. Zalevsky, "Full field of view superresolution
imaging based on two static gratings and white illumination", Appl. Opt. 47(17)
3080 (2008).
D. Cojoc, E. Ferrari, V. Garbin, E. Di Fabrizio, H. Amenitsch, M. Rappolt, B. Sartori,
P. Laggner, M. Burghammer, C. Riekel, "Scanning x-ray microdiffraction of
optically manipulated liposomes", Appl. Phys. Lett. 91 234107 (2007).
D. Cojoc, E. Ferrari, F. Difato, R. Shahapure, J. Laishram, M. Righi, E. Di Fabrizio,
Vincent Torre, "Properties of the force exerted by filopodia and lamellipodia and
the involvement of cytoskeletal components", PLoS ONE 2(10): e1072.
doi:10.1371/journal.pone.0001072 (2007).
H. Amenitsch, E. Ferrari, M. Rappolt, B. Sartori, P. Laggner, V. Garbin, E. Di
Fabrizio, M. Burghammer, C. Riekel, D. Cojoc "Development of optical tweezers
for sample fixing in microdiffraction experiments" ESRF, Newsletter 45 26 (2007)
M. Mihailescu, A. Preda, D. Cojoc, L. Preda E. Scarlat, I.M. Popescu, "Intensity
redistribution in diffractive pattern due to fractal phase changes", J. Optoel. Adv.
Mat. June 2007.
Moradi, E. Ferrari, V. Garbin, E. Di Fabrizio, D. Cojoc, "Strength control in multiple
optical traps generated by means of diffractive optical elements",.Optoelectronics
and Advanced Materials - Rapid Communications 1 158 (2007).
V. Garbin, D. Cojoc, E. Ferrari, E. Di Fabrizio, M. Overvelde, M. Versluis, S. van der
Meer, N. de Jong, D. Lohse "Changes in microbubble dynamics near a boundary
revealed by combined optical micromanipulation and high-speed imaging", Appl.
Phys. Lett. 90 114103 (2007).
M. Mihailescu, A. Preda, D. Cojoc, E. Scarlat, L. Preda, "Diffractive patterns
correlation with shape and structure of imprint objects", J. Optoel. Adv. Mat. 9
1071 (2007).
A. Alexandrescu, D. Cojoc, and E. Di Fabrizio, "Mechanism of Angular Momentum
Exchange between Molecules and Laguerre-Gaussian Beams", Phys. Rev. Lett. 96
243001 (2006)
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9.Criteri di valuatazione delle scuole di dottorato
Si riportano di seguito i criteri di valutazione delle scuole di dottorato utilizzati dal nucleo previa
approvazione degli organi accademici (questi sono i criteri adottati per la valutazione del 2008).
1. INTRODUZIONE E RIFERIMENTI LEGISLATIVI
L‟attività di valutazione dei requisiti di idoneità dei corsi di dottorato di ricerca viene effettuata dal
Nucleo di Valutazione (entrato in carica per il triennio 2006-2009 con DR 102/07 del 23/01/07 e
riconfermato per il triennio 2009-2012 con D.R. 1817/09) in adempimento al disposto di cui al
primo comma dell'art.3 del D.M. 30 aprile 1999, n. 224.I riferimenti legislativi sono i seguenti:
 D.M. 30 aprile 1999 n. 224 “Regolamento in materia di Dottorato di ricerca”, art.3 comma 2;
 Circolare M.I.U.R (Dipartimento per l‟Università, l‟Alta Formazione Artistica, Musicale e
Coreutica e per la Ricerca Scientifica e Tecnologica – Direzione Generale per l‟Università Ufficio IX) prot. 323, dd. 21/02/2006;
 Regolamento in materia di Dottorato di Ricerca dell‟Università degli Studi di Trieste (D.R. n.
908/AG dd. 08/10/99, ultima modifica D.R. n. 1867/2006 dd. 30/10/06);
 Regolamento Generale per le Scuole di Dottorato dell‟Università di Trieste (D.R. n.1188/2006
dd. 14/7/06).
I requisiti di idoneità, in base ai quali sarà condotta la valutazione dei Corsi di Dottorato, sono
quelli previsti dal D.M. 224/99:
a) la presenza nel collegio dei docenti di un congruo numero di professori e ricercatori
dell'area scientifica di riferimento del corso;
b) la disponibilità di adeguate risorse finanziarie e di specifiche strutture operative e
scientifiche per il corso e per l‟attività di studio e di ricerca dei dottorandi;
c) la previsione di un coordinatore responsabile dell‟organizzazione del corso, di un
collegio di docenti e di tutori in numero proporzionato ai dottorandi e con
documentata produzione scientifica nell‟ultimo quinquennio nell‟area di riferimento del
corso;
d) la possibilità di collaborazione con soggetti pubblici o privati, italiani o stranieri, che
consenta ai dottorandi lo svolgimento di esperienze in un contesto di attività lavorative;
e) la previsione di percorsi formativi orientati all'esercizio di attività di ricerca di alta
qualificazione presso università, enti pubblici o soggetti privati;
f) l‟attivazione di sistemi di valutazione relativi alla permanenza dei requisiti di cui al
presente comma, alla rispondenza del corso agli obiettivi formativi di cui all‟articolo 4,
anche in relazione agli sbocchi professionali, al livello di formazione dei dottorandi;
e il requisito aggiuntivo previsto dal regolamento di Ateneo:

la presenza nel Collegio dei docenti (…) di almeno dieci docenti di ruolo presenti in
maniera esclusiva in quel Collegio.
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Il MIUR (circolare Prot.323, dd. 21/2/2006), “al fine di consentire l‟adozione di criteri omogenei
di valutazione da parte dei Nuclei e di disporre di informazioni in grado di permettere un‟analisi
comparativa dello stato dei dottorati nelle diverse sedi e nei diversi ambiti disciplinari”, ha inviato
ai Nuclei di Valutazione uno schema di valutazione, proposto dal Comitato Nazionale per la
Valutazione del Sistema Universitario, da utilizzare per la verifica dei requisiti di idoneità dei
dottorati di ricerca di ciascuna sede.
Tale schema, confermato anche dalla circolare MIUR n.124 del 2/2/2007, consiste in un
questionario di domande che vanno a definire i requisiti del D.M. 224/99 in modo puntuale e
operativo, individuando alcuni aspetti che i Nuclei devono analizzare per le loro valutazioni.
I Nuclei sono, quindi, chiamati dal Ministero a formulare un giudizio motivato e ad esprimere
una valutazione di merito su ciascun dottorato per ciascuno degli aspetti individuati.
Per quanto riguarda le Scuole di Dottorato, in base all‟art.2, comma 8 del relativo regolamento,
oltre ai requisiti già individuati dal D.M. 224/99, è necessario verificare la sussistenza dei seguenti
ulteriori requisiti:
g) l‟esistenza di un progetto formativo e di ricerca che illustri dettagliatamente gli obiettivi
scientifico-formativi relativamente a tematiche sufficientemente ampie e, ove possibile, a
carattere interdisciplinare;
h) l‟inserimento dei percorsi formativi all‟interno di documentate iniziative di collaborazione
internazionale anche attraverso il coinvolgimento di una qualificata docenza internazionale
i) la documentata esistenza di iniziative rivolte ad attirare finanziamenti da parte di Enti
pubblici e privati finalizzati all‟erogazione di borse di studio e di contributi di funzionamento;
j) la documentata esistenza di iniziative rivolte alla pubblicizzazione verso l’esterno sia delle
modalità di selezione e accesso sia delle attività scientifico-didattiche, allo scopo di ottimizzare
l‟attrattività della Scuola, con particolare attenzione per i potenziali candidati stranieri.
Inoltre, in base all‟art.4.2, è previsto tra gli organi della Scuola un Consiglio Scientifico costituito
da almeno 5 componenti.
Infine, ai sensi dell‟art.5 “1. La Scuola di Dottorato può articolarsi in indirizzi. L’articolazione in
indirizzi, che deve essere giustificata sulla base di adeguate motivazioni scientifico-culturali e che
deve riferirsi ad un programma formativo relativo a un ambito disciplinare sufficientemente ampio
(…).
2. Un indirizzo può essere attivato soltanto qualora: (…) b. sussista nel Collegio la presenza di
almeno quattro docenti non soprannumerari afferenti ai settori scientifico-disciplinari
caratterizzanti l’indirizzo in modo esclusivo; c. siano assegnate all’indirizzo almeno due borse.”
2. PROCEDURA DI VALUTAZIONE
2.1 Presentazione delle domande e tempistica della valutazione
Le domande di attivazione di Scuole di Dottorato e di Istituzione/Rinnovo di Corsi di
Dottorato di Ricerca sono state presentate tramite una nuova procedura elettronica
realizzata a cura dalla Divisione ISI denominata ADO, nel periodo dal 22 febbraio 2010 al 22
marzo 2010. Per facilitare il lavoro di compilazione, sono state inserite all’ interno della
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206
procedura ADO, a cura dell’ Ufficio Studi per la Valutazione tutte le domande di
istituzione/rinnovo presentate in forma cartacea per il XXV ciclo (2009).
All’inizio della scheda di proposta, sono stati comunicati i criteri che il Nucleo avrebbe
utilizzato in sede di valutazione:
“ - Produzione scientifica del Collegio Docenti come elemento fondamentale per qualificare le
proposte di Dottorato di Ricerca (3° requisito DM.224/99). Il Nucleo potrà utilizzare a tale scopo
le informazioni presenti nella banca dati SAPERI, ma desidera che ciascun componente del
collegio docenti, in una logica di autovalutazione, indichi sulla propria scheda (allegato1)
esclusivamente le 3 pubblicazioni che ritiene più significative degli ultimi 5 anni nell’area di
riferimento del dottorato;
- congruo numero di docenti dell’Ateneo coinvolti nel collegio (1° requisito DM.224/99);
- rapporto del dottorato con il mondo del lavoro, valutato in termini di profilo professionale che si
intende formare nonché di monitoraggio dell’inserimento lavorativo di coloro che hanno
conseguito il titolo e di verifica della relazione tra gli obiettivi formativi e le specifiche posizioni
lavorative (4° requisito DM.224/99);
- attrattività del dottorato e della sede nell’ultimo ciclo attivo (rapporto candidati presenti/posti
con borsa banditi; rapporto iscritti dottorato provenienti da altri atenei/iscritti totali);
- livello di soddisfazione dei dottorandi (informazione ricavata dal questionario inviato agli
iscritti ai corsi di dottorato nel novembre 2008);
- produzione scientifica dei dottorandi ed iniziative esistenti di pubblicizzazione e referaggio delle
tesi di dottorato;
- adeguata formulazione delle tematiche scientifiche e degli obiettivi formativi, coerentemente
con le risorse disponibili (collegio docenti, progetti di ricerca attivi, strutture di ricerca, risorse
finanziarie finalizzate al funzionamento del dottorato);
- sintesi nell’esposizione del progetto formativo e accuratezza delle informazioni fornite per la
valutazione;
- qualità del sito web dedicato al dottorato di ricerca, considerando che “il sito web del dottorato
deve essere uno strumento di comunicazione rivolto principalmente ai potenziali studenti (e come
va indicato nel bando) e deve contenere tutte le informazioni necessarie all’orientamento dello
studente”;
- internazionalizzazione, ovvero esistenza di convenzioni con atenei stranieri, attività didattiche in
lingua inglese, ecc.
- per le scuole di nuova istituzione o che chiedono il rinnovo ai sensi dell’art.3 del regolamento,
esistenza di un progetto formativo e di ricerca che illustri dettagliatamente gli obiettivi scientifico
formativi relativamente a tematiche sufficientemente ampie e, ove possibile, a carattere
interdisciplinare (art.2, comma 9, lett. g del regolamento).
Infine, limitatamente alle Scuole di Dottorato che chiedono il rinnovo ai sensi dell’art.3 del
Regolamento per le Scuole di dottorato dell'Università degli Studi di Trieste, il Nucleo dovrà
disporre anche di una relazione del Direttore che dia conto delle attività effettivamente svolte nei
tre cicli precedenti, della produttività scientifica dei docenti, degli iscritti, nonché di coloro che
nel frattempo abbiano conseguito il titolo, nonché della relazione del Consiglio Scientifico di cui
all’art.6 comma 2 del medesimo Regolamento.
Per quanto riguarda le informazioni relative a iscritti al corso di dottorato, studenti che hanno
conseguito il titolo, pubblicazioni degli iscritti, il Nucleo provvederà a reperirle autonomamente
dalle banche dati di Ateneo o sul sito del corso di dottorato che si richiede sia opportunamente
aggiornato.
Per quanto riguarda le informazioni necessarie per la verifica della permanenza dei requisiti per
i dottorati in corso di svolgimento (ex sezione III- Rinnovo dei dottorati), queste verranno
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207
richieste in una fase successiva con apposito questionario, in quanto i Nuclei sono in attesa di
indicazioni dal MIUR su quali dati dovranno essere analizzati ai fini della valutazione ex-post”.
2.2 Criteri di valutazione 2010 (XXVI ciclo)
Il Nucleo ha verificato preliminarmente che nella scheda di ogni componente del collegio
fossero state indicate solamente 3 pubblicazioni degli ultimi 5 anni, scelte tra quelle
ritenute più significative della produzione scientifica connessa con le tematiche del
dottorato.
Infatti, poiché il Nucleo ha la possibilità di visualizzare l'intera produzione scientifica dei
docenti nella banca dati Saperi/CINECA, ritiene più informativo ed efficace per la
valutazione che i docenti selezionino nella proposta la propria produzione di eccellenza.
Il Nucleo analizzerà anche con l’ausilio di opportuni indicatori, i seguenti aspetti:
− produzione scientifica
del Collegio Docenti come elemento fondamentale per qualificare le
proposte di Dottorato di Ricerca (3° requisito DM.224/99);
− congruo
numero di docenti dell’Ateneo coinvolti nel collegio (1° requisito DM.224/99);
− rapporto
del dottorato con il mondo del lavoro, valutato in termini di profilo professionale
che si intende formare (4° requisito DM.224/99);
− attrattività
del dottorato e della sede nell’ultimo ciclo attivo (rapporto candidati
presenti/posti con borsa banditi; rapporto iscritti dottorato provenienti da altri atenei/iscritti
totali);
− livello
di soddisfazione dei dottorandi (informazione ricavata dal questionario inviato agli
iscritti ai corsi di dottorato nel novembre 20081);
− produzione scientifica
dei dottorandi ed iniziative esistenti di pubblicizzazione e refe raggio
delle tesi di dottorato;
− adeguata
formulazione delle tematiche scientifiche e degli obiettivi formativi,
coerentemente con le risorse disponibili (collegio docenti, progetti di ricerca attivi, strutture
di ricerca, risorse finanziarie finalizzate al funzionamento del dottorato);
− accuratezza
− sito
delle informazioni fornite per la valutazione;
web dedicato al dottorato di ricerca.
Inoltre, come auspicato dal consiglio delle Strutture Scientifiche, il Nucleo ha valutato, oltre
alla capacità di cofinanziamento prevista per il nuovo ciclo, il cofinanziamento effettivo ex
post dell’ultimo ciclo attivo e il suo scostamento da quello inizialmente preventivato.
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208
10.Valutazione da parte del nucleo di valutazione dell’Ateneo
Criterio
ID Dottorato
tipo_rec ord
nome_dottorato
nuova_istituzione
anno_attivazione
Dipartimento
cognome _docente
nome_docente
Borse cofinanziate
Borse richieste all'Ateneo
Borse cofinanziate / Tot borse
Cofinanziamento dichiarato XXIII (o ultimo ciclo attivo)
Cofinanziamento consuntivo XXIII (o ultimo ciclo
attivo)
Valutazione cofinanzi amen to
Posti ordinari
Posti ordinari coperti da borsa (vincolo >= 50%)
Posti ordinari coperti da borsa (vincolo >= 50%)
(consuntiv o bando XXIII ciclo)
N. Componenti esclusivi Collegio (vincolo >=10)
Attrattività del dottorato (Presenti alla prova esame
XXIII o ultimo ciclo attivo/Posti con borsa)
Attrattività della sede (Iscritti altro Ateneo/Totale
iscritti XXIII o ultimo ciclo attivo)
Valutazione attrattività della sede
Composizione Collegio docenti eProduzione
Scientifica (1° e 3° requisito DM 224/99)
Obiettivi formativi e progetto scientifico (1° requisito
DM 224/99)
Disponibilità risorse e strutture (2° requisito DM
224/99)
Rapporto del dottorato con il mondo lavoro (4°
requisito DM 224/99)
Percorsi formativi orientati alla ricerca (5° requisito
DM 224/99)
Valutazione sistemi di valutazione (6° requisito DM
224/99)
Valutazione sito web
Soddisfazione dottorandi (punteggio 1- 5 questionario
novembre 2009)
Valutazione produzione scientifica dottorandi
Valutazione complessi va
Valutazione
1028
Scuola di dottorato senza indirzzi
NANOTECNOLOGIE
Rinnovo
2006
Dipartimento di Fisica
Fermeglia
Maurizio
5,00
5,00
0,50
0,50
0,50
POSITIVO
17
50,00%
52.94%
34
2,22
40,00%
media
OTTIMO
POSITIVO
MOLTO POSITIVO
POSITIVO
POSITIVO
POSITIVO
OTTIMO
3.05
OTTIMO
A+ (OTTIMO)
Il documento completo di valutazione di tutte le scuole di dottorato dell‟università di Trieste è
riportato in allegato. Questo documento è utile per posizionare la scuola anche in relativo rispetto
alle altre scuole e corsi di dottorati valutati con lo stesso metro.
Scuola di dottorato di ricerca in Nanotecnologie: attività 2009
209
11. Giudizi dei referee esterni per dottorandi ammessi all’esame
finale
Di seguito sono riportati i referee esterni scelti dal collegio per ciascuno dei dottorandi:
Dottorando/a
Titolo tesi
Supervisore/Tutore
Matteo Maria Reattività e modalità di Luca Gregoratti
Dalmiglio
ossidazione di nanocluster Alberto Morgante
supportati di catalizzatori.
Paolo Lacovig
Electronic
morphology
and
Reviewer
Herbert
Over,
University D
Liebig
structure, Alessandro Baraldi Svetlozar Surnev, Karlchemical Silvano Lizzit
Franzens-Universitaet
reactivity of nanoclusters and
low-dimensional systems: fast
photoemission
spectroscopy
studies.
Graz, A
Barbara
Lorenzut
Sviluppo
di
nanostrutturati
produzione e
dell‟idrogeno.
Alessandro
UNIUD, I
Giovanni
Maria Pavan
Analisi energetica e strutturale Sabrina Pricl
di sistemi organici e inorganici.
Le
potenzialità
della
modellistica molecolare.
Paola Posocco
Simulazione
molecolare Maurizio Fermeglia Peter
Cummings,
multiscala
di
sistemi Sabrina Pricl
Vanderbilt Univ, Oak
nanostrutturati
Ridge Nat. Lab, US
catalizzatori Paolo Fornasiero
per
la Tiziano Montini
purificazione
Andrea Travan Nanocomposite systems based Sergio Paoletti
on metal nanoparticles and Ivan Donati
polysaccharides for biomedical
Trovarelli,
Ursula Röthlisberger Swiss
Fed. Inst. of Techn.,
Lausanne, CH
Martha
Lilley,
fisico,
centro nanotech “CSEM”
di Neuchatel, CH
applications.
Andrea Umeri
Analisi dello stato tensionale e Valter Sergo
cristallografico in relazione al
processo di invecchiamento in
compositi
sub-micrometrici
Zirconia – Allumina
Wolfgang
Oximatec, D
Burger,
Vedi dettaglio negli allegati.
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210
12.Presentazioni dei dottorandi al congesso del 2009
Le presentazioni PPT dei dottorandi al congresso del 2010 sono riportate negli allegati.
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