Nicola LUCHI, born in Florence - Istituto per la Protezione

CURRICULUM VITAE ET STUDIORUM
Nicola LUCHI, born in Florence (Italy), 27th December 1975.
PRESENT POSITION
Since 2011 is Researcher at the Institute for Sustainable Plant Protection (National Research Council) (IPSPCNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino – Firenze, ITALY.
Tel.: +39 0555225531
Fax: +39 0555225666
e-mail: [email protected]
EDUCATION AND TRAINING
2011: post-doctoral position at the Institute for Plant Protection (National Research Council-Florence).
2004-2010: post-doctoral position at the Department of Agriculture and Biotechnology, University of Florence.
2004: Ph.D. in Microbial Agricultural Biotechnology, University of Florence.
2000: Graduated in Forestry and Environmental Sciences, University of Florence.
During his career he resulted winner of the following grants and projects:
2010: “Phytophthora disease in nurseries and particularly on woody plants from the Mediterranean area”
(Short Term Scientific Mission; grant funded by COST). (COST Established and Emerging Phytophthora:
Increasing Threats to Woodland and Forest Ecosystems in Europe (COST FP0801). Istituto Agroforestal
Mediteraneo, Universidad Politecnica de Valencia, Valencia, Spain.
2010: “Characterization of fungal pathogens of grapevine and pine by using High Resolution Melting Analysis”.
Grant funded by Consorzio Interuniversitario per le Biotecnologie (C.I.B -Borse C.I.B. di Ricerca e Formazione
Avanzata).
2007-2009: “Molecular diagnosis of fungal endophytes and oomycetes in forest trees”. Grant funded by the
University of Florence.
2004 and 2006: two grants funded by Consorzio Interuniversitario per le Biotecnologie (C.I.B), for stay in
foreign labs (Finnish Forest Research Institute, Helsinki).
MEMBER OF COMMITTEE
Member of Management Committee COST Action FP1103 “Fraxinus dieback in Europe: elaborating guidelines
and strategies for sustainable management (FRAXBACK)” (European Cooperation in Science and Technology
– COST; 2013-2016).
RESEARCH ACTIVITIES
Research Sectors
The main research is the study of fungal pathogens by using molecular techniques, mainly based on real –
time PCR. At the present he is working on biology, epidemiology and detection of invasive fungal pathogens
(Heterobasidion irregulare, Chalara fraxinea, Fusarium circinatum and Ceratocystis platani) in Mediterranean
area. In this context the association between invasive insect (Leptoglossus occidantalis) and the native
pathogen (Diplodia sapinea) has been studied. He also optimized real-time PCR assay for the early detection
of fungal pathogens in nurseries and in forests. Quantitative PCR assay, based on TaqMan chemistry, were
developed to quantify pathogens at early stage of colonization in the following pathosystems: Diplodia
sapinea/ Pinus, Biscogniauxia mediterranea/ Quercus, B. nummularia/ Fagus, Ophiostoma novo-ulmi/Ulmus
and Phytophthora spp. The High Resolution Melting Analysis has been performed to distinguish strictly related
fungal species, such as D. sapinea and D. scrobiculata on pine shoots. He was also involved in population
studies of fungal pathogens such as Heterobasidion annosum, H. abietinum, D. sapinea and Caliciopsis pinea.
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He also studied induced systemic resistance on conifer species after infections caused by crown and root
pathogens (D. sapinea, H. annosum and H. irregulare).
Recent Scientific Activities
He is Responsible of Research Unit within the National Project (FIRB - DEFINE) “Assessing the impacts of
invasive fungal pathogens and phytophagous insects on native plants, pathogens, phytophagous insects and
symbionts”, where the differences between native and invasive insects and fungal pathogens are studied. This
research includes the analysis of plant transcripts by using RT-qPCR on Pinus pinea infected with native (H.
annosum) and invasive (H. irregulare) fungal pathogens. He also worked within the European Project ISEFOR
(Increasing Sustainability of European Forests: Modelling for Security Against Invasive Pests and Pathogens
under Climate Change), developing molecular assay to detect alien fungal species in forest trees.
POST DEGREE EXPERIENCE
He established national and international cooperation with:
a) Department of Biochemical chemistry, University of Florence, (since 2001) where he developed the studies
of real-time PCR on forest trees;
b) The Ohio State University, Columbus, Ohio, USA (2003) where he studied the resistance induced (SIR), on
Pinus nigra inoculated with Sphaeropsis sapinea and Diplodia scrobiculata;
c) Finnish Forest Research Insitute, Helsinki (2004), molecular characterization of H. annosum s.s. population
in central Italy and Europe, by using RAMs;
d) Norwegian Forest Research Insitute, Oslo, (2005), studies on H. annosum on Norway spruce by using realtime PCR;
e) Finnish Forest Research Insitute, Helsinki (2006), molecular characterization of H. abietinum population in
Europe.
f) Istituto Agroforestal Mediteraneo, Universidad Politecnica de Valencia (Spain) (2009) studying Phytophthora
disease in nurseries and particularly on woody plants from the Mediterranean area.
PROJECTS
2013: Responsible of Research Unit within the National Project “Assessing the impacts of invasive fungal
pathogens and phytophagous insects on native plants, pathogens, phytophagous insects and symbionts” FIRB
– DEFINE - PROGRAMMA "FUTURO IN RICERCA" (2013-2016).
2013: Responsible of Research Unit within the Multidisciplinary Training Programme “Science for DIPLOMAzia
– Project DIPLOMA” financed by Italian Ministry (MAE -2013).
2002: Responsible of the Project “The use of real-time PCR to monitoring Biscogniauxia mediterranea on
Quercus cerris in water stress conditions”. Project funded by University of Florence.
Participant in local, national and international research projects:
2011-2013: ISEFOR – “Increasing Sustainability of European Forests: Modelling for Security Against Invasive
Pests and Pathogens under Climate Change”.
2012: CON.MAL.VI.- “Specie invasive e cambiamento climatico: due minacce per il vivaismo pistoiese che
richiedono nuove strategie per il controllo delle malattie delle piante”. Funded by Cassa di Risparmio di Pistoia
e Pescia.
2009-2011: PRIN - “Ecological impact, epidemiology and management of new introduced species of
Heterobasidion in Italian pinewoods”. Funded by Italian Ministry (MIUR).
2009: PINITALY – “The restart of the Pine Nuts production in Italy by new pest control strategies”. Supported
by the Italian Ministry of Agricultural, Food and Forestry.
2006-2009: DIGESFAM – “Protection and management of Mediterranean beech forests”. Funded by the
Italian Ministry for Agriculture and Forestry (MIPAF).
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TEACHING
2007-2008: ‘Monitoring in forest ecosystems‘ (integrated course of M.Sc. Degree course in Science and
Phytopathological Techniques), and ‘ Monitoring systems in forest ’ (integrated course in M.Sc. degree in
Forest Pathology) Faculty of Agriculture, University of Florence.
Since 2003 he is Co-supervisor of M.Sc. Degree thesis, Forest Pathology, Faculty of Agriculture, University of
Florence.
External examiner in Master Thesis (University of Pretoria, South Africa).
Lecturing on phytopathological courses ‘Regione Toscana’ (ARSIA-META, 2008), ‘Provincia di Firenze’
(FLORA, 2004; QUALITAS – ARETUSA,2006) and ‘Plant nurseries’ (CIPA-AT and COLDIRETTI, Pistoia
2006).
INVITED LECTURER
2014: "Real-time PCR; a useful tool for early detection of invasive alien pest and pathogens" lecturer in a
Training School 'Identification and Molecular Detection of lnvasive Alien Species (lAS) in Forest Ecosystems’
(Pathway Evaluation and pest Risk Management In Transport (PERMIT) - Forests, their Products and Services
(FPS) – Action COST FP1002, 8-10th July 2014, Forest Research lnstitute (lBL) in Sekocin Stary, Poland.
2014: “Early detection of forest pathogens by using real-time PCR” lectuter in the workshop "Quantitative PCR
– applicable and efficient tool for phytopathology" 3rd to 5th September 2014 Faculty of Forestry and Wood
technology, Mendel University, Brno, Czech Republic.
2008: Invited speaker: “The real –time quantitative PCR assay: from its detection of fungi pathogenic on trees
to its use detecting AMF”. COST Action 870 “Mycorrhiza application in sustainable agricolture and natural
systems”, WG 2 e 4. 17-19 September 2008. University of Thessaloniki, Thessaloniki, Greece.
REFEREE
He is referee on International and National journals: PLoS ONE, Phytopathologia Mediterranea, Micologia
Italiana, Forest@, Forest Pathology, Australasian Plant Pathology, Molecular Ecology Resources, iForest,
FEMS Microbiology Letters, European Journal of Plant Pathology.
PUBBLICATIONS AND CONGRESS
He published 94 national and international papers, book chapters and Proceedings. He also published 24
papers on ISI journals with impact factor. He attended National and International Congress in Plant Pathology
and Forest Pathology (included IUFRO and COST meetings), and Biotechnology with oral presentations and
posters.
Results of the research were presented to the following national and international meetings and Congress:
-Convegno Nazionale SIPAV;
-Convegno Nazionale Micologia;
-Congresso Nazionale SISEF;
-IUFRO WP 7.03.10, Methodology of Forest Insect and disease survey in central Europe;
-IUFRO WP 7.02.02, Foliage, shoot and stem disease of Forest trees;
-IUFRO Unit 6.14.00, Urban Forestry;
-IUFRO WP 7.02.01, Root and Butt Rots of Forest Trees;
-COST Action in IE0601, Wood Science for conservation of cultural heritage;
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-COST Action 870, Mycorrhizal application in sustainable agriculture and natural systems;
-COST Action FP0801, Established and Emerging Phytophthora: Increasing Threats to -Woodland and Forest
Ecosystems in Europe;
-International Workshop on grapevine trunk Disease;
-Mediterranean Phytopathological Union;
-NEOBIOTA, European Conference on Invasive alien species.
PUBBLICATIONS LIST
Papers in International Journals
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Pollastrini M., Luchi N., Michelozzi M., Gerosa G., Marzuoli R., Bussotti F., Capretti P. (2015). Early
physiological responses of Pinus pinea L. seedlings infected by Heterobasidion sp.pl. in an ozone
enriched atmospheric environment. Tree Physiology (in press).
Luchi N., Oliveira Longa C.M., Danti R., Capretti P., Maresi G. (2014). Diplodia sapinea: the main fungal
species involved in the colonization of pine shoots in Italy. Forest Pathology. doi: 10.1111/efp.12109
Gonthier P., Anselmi N., Capretti P., Bussotti F., Feducci M., Giordano L., Honorati T., Lione G., Luchi
N., Michelozzi M., Paparatti B., Sillo F., Garbelotto M (2014). An Integrated Approach to Monitor and
Control the Exotic Forest Pathogen Heterobasidion irregulare in Europe. Forestry, 84, 471-481.
Luchi N., Ghelardini L., Belbahri L., Quartier M., Santini A. (2013). Rapid detection of Ceratocystis platani
inoculum by quantitative real-time PCR. Applied and Environmental Microbiology. 79 (17), 5394-5404.
Mancini V., Dapporto L., Baracchi D, Luchi N., Turillazzi S, Capretti P. (2013) Phenotypic
characterization of cryptic Diplodia species by MALDI-TOF MS and the bias of mycelium age. Forest
Pathology DOI: 10.1111/efp.12054.
Ceccherini MT, Luchi N., Pantani OL , Ascher J, Capretti P., Pietramellara G. (2013). Upward the
movement of Verticillium dahliae from soil to olive plants detected by real-time PCR. World Journal of
Microbiology and Biotechnology. 29: 1961-1967.
Luchi N., Mancini V., Feducci M., Santini A., Capretti P. (2012). Leptoglossus occidentalis and Diplodia
pinea: a new insect-fungus association in Mediterranean forests. Forest Pathology, 42: 246-251.
Luchi N., Capretti P., Fossdal C.G.,Pazzagli M., Pinzani P. (2012). Laser microdissection on Norway
Spruce bark tissue: a suitable protocol for subsequent real-time PCR analysis. Plant Biosystems, Vol.
146, 1, 92-98.
Luchi N., Paffetti D., Korhonen K., Hantula J., Capretti P. (2011). Genetic variation of Heterobasidion
abietinum in southern Europe and the Mediterranean Basin. Forest Pathology,41: 270-280.
Luchi N., Pratesi N., Simi L., Pazzagli M., Capretti P., Scala A., Slippers B., Pinzani P. (2011). HighResolution Melting Analysis: a new molecular approach for the early detection of Diplodia pinea in
Austrian pine. Fungal Biology , 115:715-723.
Luchi N., Pepori A., Capretti P., Santini A. (2011). Sarcodontia pachyodon: a Canker and White-rot Agent
of plane-trees. Journal of Phytopathology. 159,2,117-119.
De Vita P., Serrano M.S., Luchi N., Capretti P., Trapero A., Sánchez M.E. (2010). Susceptibility of Abies
pinsapo and its tree cohort species to Heterobasidion abietinum. Forest Pathology, 40: 129–132.
Comparini C., Carresi L., Pagni E., Sbrana F., Sebastiani F., Luchi N., Santini A., Capretti P., Tiribilli B.,
Pazzagli L., Cappugi G., Scala A. (2009). New proteins orthologous to cerato-platanin in various
Ceratocystis species and the purification and characterization of cerato-populin from Ceratocystis
populicola. Applied Microbiology and Biotechnology, 84:309-322.
Luchi N., Sanchez M.E., Capretti P. (2008). Preliminary notes on the population structure of
Heterobasidion abietinum from Abies pinsapo in Spain. Phytopathologia Mediterranea 47,277-281.
Bonello P., Capretti P., Luchi N., Martini V., Michelozzi M. (2008). Systemic effects of Heterobasidion
annosum s.s. infection on severity of Diplodia pinea tip blight and terpenoid metabolism in Italian stone
pine (Pinus pinea L.). Tree Physiology 28, 1653-1660.
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16. Luchi N., Capretti P., Bonello P. (2007) Production of Diplodia scrobiculata and D. pinea pycnidia on
ground Austrian pine needle agar medium. Phytopathologia Mediterranea 46, 230-235.
17. D’Amico L., Motta E., Annesi T., Scirè M., Luchi N., Hantula J., Korhonen K., Capretti P. (2007) The North
American P group of Heterobasidion annosum s.l. is widely distributed in Pinus pinea forests of the
western coast of central Italy. Forest Pathology 37, 303-320.
18. Sanchez M.E., Luchi N., Jiménez J.J., de Vita P., Sanchez J.E., Trapero A., Capretti P. (2007) An isolated
population of Heterobasidion abietinum on Abies pinsapo in Spain. Forest Pathology 37, 348-356.
19. Sanchez M.E., Luchi N., Sanchez J. E., Jimenez J. J., Trapero A., Capretti P. (2007) La podredumbre
radical del pinsapo I: Identificación específica del agente causal. Bol. San. Veg. Plagas, 33, 529-537.
20. Maresi G., Luchi N., Pinzani P., Pazzagli M., Capretti P. (2007) Detection of Diplodia pinea in
asymptomatic pine shoots and its relation to the Normalized Insolation index. Forest Pathology 37, 272280.
21. Luchi N., Capretti P., Vettraino A.M., Vannini A., Pinzani P., Pazzagli M. (2006). Early detection of
Biscogniauxia nummularia in symptomless European beech (Fagus sylvatica L.) by TaqMan™ real-time
PCR. Letters in Applied Microbiology 43, 33-38.
22. Luchi N., Capretti P., Pinzani P., Orlando C., Pazzagli M. (2005). Real-time PCR detection of
Biscogniauxia mediterranea in symptomless oak tissue. Letters in Applied Microbiology 41, 61-68.
23. Luchi N., Ma R., Capretti P., Bonello P. (2005) Systemic induction of traumatic resin ducts and resin flow
in Austrian pine by wounding and inoculation with Sphaeropsis sapinea and Diplodia scrobiculata. Planta
221, 75-84.
24. Luchi N., Capretti P., Surico G., Orlando C., Pazzagli M., Pinzani P. (2005). A real-time quantitative PCR
assay for the detection of Sphaeropsis sapinea from inoculated Pinus nigra shoots. Journal of
Phytopathology 153, 37-42.
Papers in National Journals
1. Luchi N., Korhonen K., Hantula J., Capretti P. (2009). Variabilità di Heterobasidion abietinum in Europa e
nel bacino del Mediterraneo. Micologia Italiana XXXVIII (2), 40-44.
2. Luchi N., Vannuccini M., Panzavolta T., Tiberi R., Feducci M., Salbitano F., Giachini M., Zocco Pisana L.,
Capretti P. (2008). Censimento e indicazioni gestionali contro le avversità delle alberature dell’Opera delle
Mura di Lucca. Forest@ 5, 253-261.
3. Luchi N., Mazza G.L., Feducci M., Capretti P. (2007). I funghi lignicoli del faggio. Micologia Italiana,
XXXVI (2):44-48.
4. Luchi N., Barzanti G.P., Emanueli A., Stella I., Capretti P. (2005). Disseccamenti del cedro causati da
Sphaeropsis sapinea. Informatore Fitopatologico 5, 43-46.
5. Wolynski A., Maresi G., Ambrosi P., Luchi N., Capretti P. (2004). Sphaeropsis sapinea e rimboschimenti
di pino nero in Trentino. Sherwood 102, 13-17.
6. Capretti P., Menguzzato G., Maresi G., Luchi N., Moriondo F. (2003). Fenomeni di deperimento e di moria
in popolamenti artificiali misti di latifoglie e conifere. Annali Accademia Italiana di Scienze Forestali, Vol.
LII, 3-30.
7. Luchi N. (2001). Alcune osservazioni sugli ascomiceti deboli parassiti colonizzatori del legno di faggio.
Monti e Boschi 2, 14-16.
Book Chapters
1. Moricca S., Luchi N. (2009). Diagnostica di laboratorio. In: Elementi di Patologia Forestale. Ed. Capretti
P., Ragazzi A. Patron Editore, Bologna, pp. 58-75. ISBN 978-88-555-3042-2.
2. Capretti P., Luchi N., Ragazzi A., Turchetti T. (2002). Principali agenti patogeni nei soprassuoli governati
a ceduo in Italia. In : Il bosco ceduo in Italia. Ed. Ciancio O., Nocentini S. Accademia Italiana di Scienze
Forestali, Firenze, pp. 343-364. ISBN 88-87553-04-1.
3. Luchi N., Capretti P., Pazzagli M., Pinzani P. (2004). Real-time quantitative PCR detection of the
endophytic occurrence of Biscogniauxia mediterranea in oaks. In: Endophytism in forest trees. Ed.
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Ragazzi A., Moricca S., Dellavalle I. Accademia Italiana di Scienze Forestali, Firenze, pp.169-179. ISBN
88-87553-07-6.
Presentations and Meetings
1. Migliorini D., Tondini E., Luchi N., Ghelardini L., Capretti P., Santini A. (2014). Detection of Phytophthora
species on different woody species in nurseries. In: Phytophthora in Forest and Natural ecosystems. 7th
meeting IUFRO, working party 7.02.09. 10th-1th November, Esquel, Chubut, Patagonia Argentina. (Poster)
2. Pepori A.L., Michelozzi M., Santini A., Cencetti G., Luchi N. (2014): Comparative impact of alien and native
fungal tree pathogens on Pinus pinea. 8th International Conference on Biological Invasions (NEOBIOTA
2014). Biological Invasions: From understanding to action. 3-8 November 2014, Antalya, Turkey. (Poster)
3. Luchi N., Ghelardini L., Pepori A.L., Migliorini D., Santini A. (2014). Detection and epidemiology of invasive
fungal pathogens by real time quantitative PCR. XXIV IUFRO WORLD CONGRESS. Sat Lake City, Utah,
5-11 October 2014. (Poster)
4. Feducci M., Ghelardini L., Luchi N., Pepori A., Santini A., Capretti P. (2014) Monitoring, detection and
management of Ceratocystis platani in urban contest. European Conference of Arboricolture. Turin, May
26-28 2014 (Poster)
5. Capretti P., Luchi N., Migliorini D. (2013) Diplodia pinea and D. scrobiculata diffusion on Douglas fir in
Tuscany (Italy) Proceedings: Biosecurity in natural forests and plantations, genomics and biotechnology for
biosecurity in forestry. IUFRO WP 7.02.02. 20th – 25th May, 2013, Brno and Cerna Hora, Czech Republic.
(Poster)
6. Luchi N., Carrari E., Capretti P., Feducci M., Santini A. (2013). Chalara fraxinea in Mediterranean
environment. FRAXBACK Malmo (‘Frontiers in ask dieback’) COST Action FP1103 FRAXBACK 4th MC
Meeting & Workshop “Frontiers in ash dieback research”, 4-6th of September 2013, Sankt Gertrud
Konferens, Malmö, Sweden (oral presentation).
7. Capretti P., Carrari E., Feducci M., Luchi N., Santini A. (2013). Relationship with Chalara fraxinea and
Mediterranean environment. XIX Convegno Nazionale SIPaV, 23-25 Settembre 2013, Padova. (Poster).
Journal of Plant Pathology, 95, (4, Supplement), S4.37.
8. Gonthier P., Luchi N., Petrucco Toffolo E., Balestrini R., Colazza S., Faccoli M., Garbelotto M., Giorcelli A.,
Giordano L., Guarino S., Lione G., Loreto F., Mello A., Michelozzi M., Pepori A.L., Santini A., Sillo F.,
Vizzini A. and Zampieri E. (2013). DEFINE: a multidisciplinary research project to investigate the effects of
exotic plant pathogenic fungi and insects on native ecosystems (2013). XIX Convegno Nazionale SIPaV ,
23-25 Settembre 2013, Padova. (Poster). Journal of Plant Pathology, 95, (4, Supplement), S4.46.
9. P. Gonthier, N. Luchi, E. Petrucco Toffolo, R. Balestrini, S. Colazza, M. Faccoli, M. Garbelotto, S. Guarino,
L. Giordano, F. Guglielmo, G. Lione, F. Loreto, A. Mello, M. Michelozzi, A.L. Pepori, A. Santini, F. Sillo, A.
Vizzini and E. Zampieri (2013) Investigating the effects of invasive fungal plant pathogens and
phytophagous insects on native plants, pathogens, phytophagous insects and symbionts: toward an holistic
understanding of biological invasions. 10th International Congress of Plant Pathology (ICPP 2013), Beijing,
China, August 25-30, 2013. Acta Phytopathologica Sinica 43 (Suppl.), 264-265. (Poster).
10. Santini A., Pepori A.L., Luchi N. (2012). Air-borne spread of Ceratocystis platani by saw dust. NEOBIOTA
2012 - Halting Biological Invasions in Europe: from Data to Decisions. 12th-14th September 2012,
Pontevedra, Spain. Pg. 255 (Poster).
11. Luchi N., Migliorini D., Capretti P., Santini A. 2012. Early Phytophthora spp. detection by qPCR. The Sixth
IUFRO Working Party 7.02.09 “Phytophthora in Forests and Natural Ecosystems”. 9th - 14th Cordoba
(Spain) September 2012. Pg.94.
12. Gonthier P., Anselmi N., Capretti P, Bussotti F., Feducci M., Garbelotto M., Giordano L.,Guglielmo F,
Honorati T, Lione G., Luchi N., Mancini V., Michelotti S., Michelozzi M.,Nicolotti G., Paparatti B., Pollastrini
M., Vettraino A.M., 2011: A comprehensive approach to investigate the invasion by Heterobasidion
irregulare in Italy and its interaction with H. annosum. In: Proceedings of XIII Conference "Root and Butt
Rot of Forest Trees" IUFRO Working Party 7.02.01 September 4th – 10th 2011 Firenze – S. Martino di
Castrozza, Italy. Pg.23.
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13. Luchi N., Paffetti D., Santini A., Capretti P., 2011: Heterobasidion in conifer forests: genetic diversity
across the Italian peninsula. In: Proceedings of XIII Conference "Root and Butt Rot of Forest Trees" IUFRO
Working Party 7.02.01 September 4th – 10th 2011 Firenze – S. Martino di Castrozza, Italy.Pg.27.
14. Luchi N., Paffetti D., Korhonen K., Hantula J., Capretti P., 2010: Genetic variation of the Heterobasidion
abietinum populations: diversification across the South Europe and Mediterranean basin. 13th Congress of
the Mediterranean Phytopathological Union. Rome, 20-25 June 2010. Petria 283-284. (oral presentation).
15. Luchi N., Capretti P., Ceccarelli B., Vettraino A. M., Vannini A., 2010: Detection of Biscogniauxia
nummularia in asymptomatic beech trees sampled in Apennine Mountains. 13th Congress of the
Mediterranean Phytopathological Union. Rome, 20-25 June 2010. Petria 209-210. (Poster).
16. Feducci M., Moriondo M., Dibari C., Nocentini A., N. Luchi, Capretti P., 2010: Influence of climatic
parameters on the spreading of the hypovirulent form of chesnut blight. 13th Congress of the
Mediterranean Phytopathological Union. Rome, 20-25 June 2010. Petria 139-140. (Poster).
17. Luchi N., Mancini V., Capretti P., 2010: Real-time PCR assay to detect Phytophtora species from plant,
soil and water. 13th Congress of the Mediterranean Phytopathological Union. Rome, 20-25 June 2010.
Petria 211-212. (Poster).
18. Luchi N., Capretti P., Mora Sala B., Abad-Campos P., Pérez Sierra A., 2010: STSM: Phytophthora disease
in ornamental nurseries after an experience in Valencia Region. In Proceedings of : COST Action FP0801
“Established and Emerging Phytophthora: Increasing threats to woodland and forest ecosystems in
Europe”. 5th Meeting Viterbo 28-30 June 2010. pg 19 (Poster).
19. Luchi N., Paffetti D., Korhonen K., Hantula J., Sanchez M.E., Do mu Lehtijärvi T., Lehtijärvi A., P. Capretti,
2010: Heterobasidion abietinum population genetic structure in respect to the European Abies population.
XVI Congresso SIPaV. Firenze, 14-17 Settembre 2010. Journal of Plant Pathology, (Poster).
20. Mancini V., Luchi N., Capretti P., 2010: Shoot and tip blight by Diplodia pinea and D. scrobiculata detected
by molecular methods on Pseudotsuga menziesii in central Italy. XVI Congresso SIPaV. Firenze, 14-17
Settembre 2010. Journal of Plant Pathology, (Poster).
21. Mancini V., Luchi N., Feducci M., Capretti P., 2010: Diplodia pinea detection on Leptoglossus occidentalis
(insect vector) by Real time-PCR. XVI Congresso SIPaV. Firenze, 14-17 Settembre 2010. Journal of Plant
Pathology (Poster).
22. Mancini V., Luchi N., Capretti P., 2010: Detection of Phytophthora cambivora in soil particles by real time
quantitative assay. XVI Congresso SIPaV. Firenze, 14-17 Settembre 2010. Journal of Plant Pathology
(Poster).
23. Luchi N., Ceccarelli B., Vettraino A.M., Vannini A., Capretti P., 2010: Molecular detection of Biscogniauxia
nummularia in asymptomatic beech trees in Apennine Mountains. XVI Congresso SIPaV. Firenze, 14-17
Settembre 2010. Journal of Plant Pathology (Poster).
24. Feducci M., Luchi N., Mancini V., Capretti P., 2010: Real time PCR detection of Diplodia pinea from
asymptomatic Pinus nigra trees related to environmental parameters. XVI Congresso SIPaV. Firenze, 1417 Settembre 2010. Journal of Plant Pathology (Poster).
25. Feducci M., Luchi N., Mancini V., Capretti P., 2010:Quantitative molecular detection of Biscogniauxia
mediterranea on Quercus cerris growing in sites predisposed to drought stress. XVI Congresso SIPaV.
Firenze, 14-17 Settembre 2010. Journal of Plant Pathology (Poster).
26. Luchi N., Mancini V., Capretti P., 2009: Detection and quantification of Phytophthora species by real-time
PCR. In Proceedings of the: Established and Emerging Phytophthora: Increasing Threats to Woodland and
Forest Ecosystems in Europe. COST - Action FP0801 Working Groups 3 & 4 Meeting. Universidade do
Algarve, Faro, Portugal, 10-12 September 2009, p.18 (Poster)
27. Luchi N., Pinzani P., Pazzagli M., Capretti P., 2009: Detection of Botryospaheriaceae species by real time
PCR. 6 th International Workshop on grapevine trunk Disease. 1-3 September 2008, Florence, Italy.
Phytopathologia Mediterranea 48, (1):163. (Poster)
28. Luchi N., Korhonen K., Hantula J., Capretti P., 2008: Variabilità di Heterobasidion abietinum in Europa e
nel bacino del Mediterraneo. In: libro degli abstract XVII Convegno nazionale di Micologia. Pavia 10-12
Novembre 2008, p. 22 (oral presentation).
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29. De Vita P., Luchi N., Capretti P., Trapero A., Sánchez M.E., 2008: Susceptibilidad de especies arboreas
acompanantes del Pinsapo a la infeccion por Heterobasidion abietinum. Panel. XIV Congreso Nacional de
la Sociedad Española de Fitopatología. 15-19 Septiember 2008. Lugo, Spain. (Poster).
30. Luchi N., Capretti P., 2008: The real –time quantitative PCR assay: from its detection of fungi pathogenic
on trees to its use detecting AMF. In: Proceedings of the COST Action 870 Mycorrhiza application in
sustainable agricolture and natural systems. Working Groups 2 and 4. University of Thessaloniki,
Thessaloniki, Greece. 17-19 September 2008, pp. 51-54. (Invited speaker).
31. Luchi N., Villari C., Capretti P., 2008: Real-time PCR in monitoring of forest tree pathogens. In: Libro degli
abstract A Universal language: decoding the communication among living organisms to develop new
biotechnological control strategies for disease. Firenze 12 Marzo 2008, p.13. (oral presentation).
32. Luchi N., Capretti P., Scala A., 2007: Detection and early identification of wood decay fungi: from standing
trees to wood substrates. In: Proceedings of the International Conference held by COST Action IE0601
Wood Science for conservation of cultural heritage. Ed. Uzielli L., Firenze, 8-10 November 2007. Firenze
University Press, 2009. Pp. 24-27. ISBN 978-88-8453-382-1(Poster).
33. Luchi N., Sánchez M.E., Capretti P., 2007: Genetic variability of Heterobasidion abietinum isolates
collected from Abies alba and A. pinsapo in Mediterranean Region. XIV Congresso SIPaV. Perugia, 18-21
Settembre 2007. Journal of Plant Pathology 89 (3): 46. (Poster).
34. Capretti P., Luchi N., Mazza G., 2007: Root rot disease by Heterobasidion annosum on Fagus Sylvatica
stands in Southern Italy. XIV Congresso SIPaV. Perugia, 18-21 Settembre 2007. Journal of Plant
Pathology 89, (3):34. (Poster).
35. Luchi N., Vannuccini M., Panzavolta T., Tiberi R., Salbitano F., Zocco Pisana L., Feducci M., Capretti P.,
2007: Avversità delle alberature delle mura di Lucca: censimento e gestione programmata. In : Libro degli
abstract VI Congresso Nazionale SISEF. La gestione delle foreste tra cambiamenti globali e azioni locali.
C.R.A. (Centro di Ricerca per la Selvicoltura), Arezzo, 25-27 Settembre 2007, p. 55 (Poster).
36. Luchi N., Sánchez M.E., Trapero A., Capretti P., 2007: Specific identification of Heterobasidion isolates
infecting Abies pinsapo in Spain. In: Proceedings of the IUFRO WP 7.02.01. Root and Butt Rots of Forest
Trees. 12th International Conference on Root and Butt Rots. Eds. M. Garbelotto, P. Gonthier. 12-19 August
2007. Berkeley, California - Medford, Oregon (USA), pp. 163-164 (oral presentation).
37. Capretti P., Luchi N., Michelozzi M., Bonello P., 2007: Are terpenes involved in cross induction of systemic
susceptibility between Heterobasidion annosum and Diplodia pinea in Italian stone pine? In: Proceedings of
the IUFRO WP 7.02.01. Root and Butt Rots of Forest Trees. 12th International Conference on Root and
Butt Rots. Ed. M. Garbelotto, P. Gonthier. Berkeley, California - Medford, Oregon (USA), 12-19 August
2007, pp. 37-39.
38. Luchi N., Capretti P., Maresi G., 2007: Detection of Diplodia pinea in asymptomatic pine shoots and cones.
In: Foliage, shoot and stem disease of Forest trees. IUFRO WP 7.02.02. Sopron (Hungary), 21-26 May
2007. Acta Silvatica & Lignaria Hungarica, 111-114. (oral presentation)
39. Capretti P., Feducci M., Luchi N., 2007: Environmental condition and Cypress canker disease. In: Foliage,
shoot and stem disease of Forest trees. IUFRO WP 7.02.02. Sopron (Hungary), 21-26 May 2007. Acta
Silvatica & Lignaria Hungarica, 155-158.
40. Luchi N., Mazza G.L., Feducci M., Capretti P., 2006: I funghi lignicoli del faggio. In: libro degli abstract XVI
Convegno Nazionale di Micologia. Firenze, 4-6 Dicembre 2006, p. 39 (oral presentation).
41. Capretti P., Luchi N., Feducci M., Mazza G., Villari C., 2006: Funghi patogeni di piante forestali: dalle
segnalazioni al monitoraggio fitopatologico. In: libro degli abstract XVI Convegno nazionale di Micologia.
Firenze, 4-6 Dicembre 2006, p. 36.
42. Luchi N., Capretti P., Pazzagli M., Pinzani P., 2006: Diagnosi precoce di malattie in piante arboree
ornamentali e forestali. O.R.B.I.T. (Osservatorio Regionale delle biotecnologie per l’innovazione
tecnologica, Regione Toscana). Borsa della Ricerca e dell’Innovazione. Festival della Creatività. Fortezza
da Basso, Firenze, 30 Novembre- 1 Dicembre 2006 (Poster).
43. Luchi N., Capretti P., Bonello P.,2006: Systemic induction of traumatic resin ducts (TRDs) in Austrian
pine by wounding and inoculation with Sphaeropsis sapinea and Diplodia scrobiculata. In: Conifer
defence (CON.DEF.) annual meeting, Firenze, 1-4 October 2006 (oral presentation).
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44. Luchi N., Capretti P., Pinzani P., Pazzagli M., 2006: Real-time PCR an useful tool for disease survey of
forest trees pathogens. In. Proceedings of the IUFRO Working Party 7.03.10 Methodology of Forest
Insect and disease survey in central Europe. Ed. Tomiczek C., Krehan H.. Gmunden (Austria), 11-14
September 2006, pp.154-157. (oral presentation).
45. Luchi N., Capretti P., Pinzani P., Orlando C., Pazzagli M., 2006: Early detection of fungal tree pathogens
by real-time PCR. In: Proceedings of the IUFRO Unit 6.14.00 Urban Forestry 9th European Forum on
Urban Forestry, Ed. Ferrini F. Salbitano F., Sanesi G., Firenze, 22-27 Maggio 2006, pp. 102-103. (oral
presentation).
46. Scala A., Comparini C., Carresi L., Luchi N., Sebastiani F., Patrocini I., Pazzagli L., Pantera B., Zoppi C.,
Cappugi G., Spisni A., Pertinhez T.A., Bernardi R., Fontana F., Salvini M., Durante M., Pane C., Scala F.,
2005: A multidisciplinary approach to study the role of Ceratoplatanin, a small protein from Ceratocystis
fimbriata f.s. platani, in the pathogenesis of the plane canker stain. XII Convegno Nazionale S.I.Pa.V.,
Villa S. Giovanni (RC), 29 Settembre- 1 Ottobre 2005. Journal of Plant Pathology 87 (4):282. (Poster).
47. Luchi N., Capretti P., Pinzani P., Orlando C., Pazzagli M., Vettraino A.M., Vannini A., 2005: Real - time
PCR: a molecular tool for early detection of fungal in forest trees. XII Convegno Nazionale S.I.Pa.V. Villa
S. Giovanni (RC), 29 Settembre - 1 Ottobre 2005. Journal of Plant Pathology 87, (4):278-279. (oral
presentation).
48. Luchi N., Capretti P., Pazzagli M., Pinzani P., Orlando C., 2004: Latent phase of Biscogniauxia
mediterranea quantification on Quercus spp. with real-time PCR by TaqManTM chemistry. XI Convegno
Nazionale S.I.Pa.V. Milano 29 Settembre- 1 Ottobre 2004. Journal of Plant Pathology 86, (4):208
(Presentazione orale)
49. Capretti P., Luchi N., Pazzagli M., Pinzani P., 2003: Application of real-time PCR to monitor the
occurence of Biscogniauxia mediterranea in oaks. In: Abstract of the Joint Workshop on Molecular
Diagnostic of Plant Pathogens. Lamezia Terme (VV), 20-23 Novembre 2003. Phytopathologia
Mediterranea 42, (3):298. (oral presentation)
50. Luchi N., Capretti P., Pazzagli M., Pinzani P., 2002: Individuazione di Sphaeropsis sapinea in tessuti
asintomatici di pino nero mediante real-time PCR. In: Libro degli Abstract V Workshop italiano di PCR
Quantitativa. Ed. Applied Biosystems. Firenze, 19 Dicembre 2002, p. 39 (Poster).
51. Luchi N., Capretti P., Pazzagli M., Pinzani P., 2002: Detection of Sphaeropsis sapinea in symptomless
pine trees by real-time PCR. In: Advanced of Plant Pathology (SIPaV), Roma 1-2 Ottobre 2002. Journal
of Plant Pathology 84, (3):186. (oral presentation)
52. Luchi N., Capretti P., Pazzagli M., Pinzani P., 2002: Individuazione di Sphaeropsis sapinea in fase
endofitica mediante l’impiego della real-time PCR. In: Atti del Convegno Nazionale L’endofitismo di funghi
e batteri patogeni in piante arboree e arbustive. Ed. A. Franceschini, F. Marras. Sassari, 19-21 Maggio
2002, pp.163-168.
53. Luchi N., Capretti P., Pazzagli M., Orlando C., 2001: Utilizzo della real-time PCR per quantificare il DNA
di Sphaeropsis sapinea in tessuti vegetali asintomatici di pino nero (Pinus nigra). In: Libro degli Abstract
IV Workshop italiano di PCR quantitativa. Applied Biosystems. Firenze, 21 Novembre 2001, p. 25
(Poster).
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