CURRICULUM VITAE, aggiornato novembre 2012 Cognome: BIAGI

CURRICULUM VITAE
Family Name: BIAGI
Jan 2017
First Name: ETTORE
Place and date of birth: Lecco, April 22nd 1970 (Italy).
Citizenship: Italian.
Current work address:
Centro di Ricerca, Fondazione M. Tettamanti,
Ospedale San Gerardo Monza, Via Donizetti 106,
Monza (MI).
Phone: 039-2332232
e-mail: [email protected]
Current home address:
Via Bachelet 15, Oggiono (Lecco)
Education
- since September 2014: Associate Professor in Pediatrics.
- since November 2012: Medical Director of the Laboratory of Cell and Gene
Therapy “Stefano Verri”, San Gerardo Hospital, Monza.
- since July 2005: Technical Director (Qualified person) of the Laboratory of Cell
and Gene Therapy “Stefano Verri”, San Gerardo Hospital, Monza.
- since January 2005: Academic Researcher at Pediatric Department
(University of Milan-Bicocca).
- since January 2005: Medical Assistant at the Pediatric Department, San
Gerardo Hospital, Monza.
- November 2000: Research Doctorate in Pediatric Hematology and Oncology
(University of Milan-Bicocca). From July 2001 to December 2004: postdoctoral
fellow at “Center for Cell and Gene Therapy”, Texas Children Hospital, Baylor
College of Medicine, Houston (Texas) – Director: Prof. MK Brenner.
- November 2000: Specialty in Pediatrics.
- July 1995: Degree in Medicine, Faculty of Medicine at "Università di Milano"
(110/110 cum laude: with Distinction). Degree-thesis: "Valuation of mechanical
and infective complications regarding the use of two different types of Central
Venous Catheters (Hickman and Groshong) in children with hematological
malignancies".
- July 1989: graduation at Liceo Classico "A. Manzoni", Lecco (specializing in
classics; final score: 58/60).
Hospital training
-
Ospedale S.Gerardo-Monza: Clinica Pediatrica-Università di Milano,
Prof. G.Masera and Prof. A.Biondi, from 1993 until July 2001, with
special attention to children with hematological malignancies receiving
polychemotherapy
treatments
and
Bone
Marrow Transplantation.
(frequency at the Day Hospital of Pediatric Hematology and department
of Hematology / Bone Marrow Transplant) and of pediatrics (activities
Paediatric First Aid, day and night wards).
-
Responsible for planning of the training of pediatric tutorial teaching in
the Faculty of Medicine at the University of Milan Bicocca (fifth and sixth
year of course).
-
Responsible for planning of the training of pediatric practitioners of
General Medicine at the Hospital San Gerardo Hospital in Monza.
-
Responsible for planning of the training of pediatric practitioners of
General Medicine at the Hospital San Gerardo Hospital in Monza.
-
Responsible for teaching courses in Biotechnology Medical / Therapy for
Molecular Biotechnology and of General Pediatrics at the Faculty of
Dentistry,
Technical
Laboratory,
Dental
Hygienists,
Technical
Radiologists, Nurses training course.
-
Scientific Responsible for the area of Pediatric Molecular Biology of the
Italian Association of Cancer Research (SIRP), http://www.sirped.it.
Publications
1. Acute Myeloid Leukemia Targeting by Chimeric Antigen Receptor T Cells:
Bridging the Gap from Preclinical Modeling to Human Studies. Rotiroti MC,
Arcangeli S, Casucci M, Perriello V, Bondanza A, Biondi A, Tettamanti S, Biagi
E. Hum Gene Ther. 2016 Dec 1. doi: 10.1089/hum.2016.092. [Epub ahead of
print]
2. Magnani CF, Turazzi N, Benedicenti F, Calabria A, Tenderini E, Tettamanti S,
Giordano Attianese GM, Cooper LJ, Aiuti A, Montini E, Biondi A, Biagi E.
Immunotherapy of acute leukemia by chimeric antigen receptor-modified
lymphocytes using an improved Sleeping Beauty transposon platform.
Oncotarget. 2016 Jun 13. doi: 10.18632/oncotarget.9955. [Epub ahead of print]
3. Gaipa G, Introna M, Golay J, Nolli ML, Vallanti G, Parati E, Giordano R,
Romagnoli L, Melazzini M, Biondi A, Biagi E. Development of advanced
2
therapies in Italy: Management models and sustainability in six Italian cell
factories.Cytotherapy. 2016 Apr;18(4):481-6. doi: 10.1016/j.jcyt.2016.01.002.
4. D, Gaipa G, Bassetti B, Cabiati B, Spaltro G, Biagi E, Parma M, Biondi A,
Cavallotti L, Gambini E, Pompilio G. Full GMP-Compliant Validation of Bone
Marrow-Derived Human CD133 (+) Cells as Advanced Therapy Medicinal
Product for Refractory Ischemic Cardiomyopathy. Belotti Biomed Res Int.
2015;2015:473159.
5. Magnani CF, Biondi A, Biagi E. Donor-derived CD19-targeted T cells in
allogeneic transplants. Curr Opin Hematol. 2015 Nov;22(6):497-502.
6. Taddio A, Tommasini A, Valencic E, Biagi E, Decorti G, De Iudicibus S,
Cuzzoni E, Gaipa G, Badolato R, Prandini A, Biondi A, Ventura A.Failure of
interferon-γ pre-treated mesenchymal stem cell treatment in a patient with
Crohn's disease. World J Gastroenterol. 2015 Apr 14;21(14):4379-84.
7. Tettamanti S, Biondi A, Biagi E, Bonnet D.CD123 AML targeting by chimeric
antigen
receptors:
A
novel
magic
bullet
for
AML
therapeutics?
Oncoimmunology. 2014 May 14;3:e28835
8. Rambaldi A, Biagi E, Bonini C, Biondi A, Introna M. Cell based strategies to
manage
leukemia
relapse:
efficacy
and
feasibility
of
immunotherapy
approaches. Leukemia. 2014 Jun 12. doi: 10.1038/leu.2014.189. [Epub ahead
of print] PMID: 24919807 [PubMed - as supplied by publisher]
9. Pizzitola I, Anjos-Afonso F, Rouault-Pierre K, Lassailly F, Tettamanti S,Spinelli
O,
Biondi
A,
Biagi
E,
Bonnet
D.
Chimeric
Antigen
Receptors
againstCD33/CD123 antigens efficiently target primary Acute Myeloid Leukemia
cells invivo. Leukemia. 2014 Feb 7. doi: 10.1038/leu.2014.62. [Epub ahead of
print] PubMed PMID: 24504024.
10. Valencic E, Loganes C, Cesana S, Piscianz E, Gaipa G, Biagi E, Tommasini A.
Inhibition of mesenchymal stromal cells by pre-activated lymphocytes and
theirculture media. Stem Cell Res Ther. 2014 Jan 9;5(1):3. [Epub ahead of
print] PubMed PMID: 24405828.
11. Introna M, Lucchini G, Dander E, Galimberti S, Rovelli A, Balduzzi A, Longoni
D, Pavan F, Masciocchi F, Algarotti A, Micò C, Grassi A, Deola S, Cavattoni I,
Gaipa G, Belotti D, Perseghin P, Parma M, Pogliani E, Golay J, Pedrini O,
Capelli C, Cortelazzo S, D'Amico G, Biondi A, Rambaldi A, Biagi E. Treatment
of Graft versus Host Disease with Mesenchymal Stromal Cells: A Phase I Study
on 40 Adult and Pediatric Patients. Biol Blood Marrow Transplant. 2013 Dec 7.
pii: S1083-8791(13)00570-3. doi: 10.1016/j.bbmt.2013.11.033. [Epub ahead of
print] PubMed PMID: 24321746.
3
12. Pischiutta F, D'Amico G, Dander E, Biondi A, Biagi E, Citerio G, De Simoni MG,
Zanier ER. Immunosuppression does not affect human bone marrow
mesenchymal stromal cell efficacy after transplantation in traumatized mice
brain.
Neuropharmacology.
2013
Nov
15;79C:119-126.
doi:
10.1016/j.neuropharm.2013.11.001. [Epub ahead of print] PubMed PMID:
24246661.
13. Belingheri M, Lazzari L, Parazzi V, Groppali E, Biagi E, Gaipa G, Giordano R,
Rastaldi MP, Croci D, Biondi A, Rebulla P, Edefonti A, Ghio L. Allogeneic
mesenchymal stem cell infusion for the stabilization of focal segmental
glomerulosclerosis. Biologicals. 2013 Oct 14.
14. Tettamanti S, Magnani CF, Biondi A, Biagi E. Acute myeloid leukemia and
novel biological treatments: Monoclonal antibodies and cell-based genemodified immune effectors. Immunol Lett. 2013 Sep 25.
15. Magnani CF, Tettamanti S, Maltese F, Turazzi N, Biondi A, Biagi E. Advanced
targeted, cell and gene-therapy approaches for pediatric hematological
malignancies: results and future perspectives. Front Oncol. 2013 Apr 30;3:106.
16. Laurin D, Marin V, Biagi E, Pizzitola I, Agostoni V, Gallot G, Vié H, Christine
Jacob M, Chaperot L, Aspord C, Plumas J. Upregulation of Adhesion Molecules
on Leukemia Targets Improves the Efficacy of Cytotoxic T Cells Transduced
With Chimeric Anti-CD19 Receptor. J Immunother. 2013 Apr;36(3):181-9.
17. Tettamanti S, Marin V, Pizzitola I, Magnani CF, Giordano Attianese GM, Cribioli
E, Maltese F, Galimberti S, Lopez AF, Biondi A, Bonnet D, Biagi E. Targeting of
acute myeloid leukaemia by cytokine-induced killer cells redirected with a novel
CD123-specific
chimeric
antigen
receptor.
Br
J
Haematol.
2013
May;161(3):389-401.
18. de Girolamo L, Lucarelli E, Alessandri G, Avanzini MA, Bernardo ME, Biagi E,
Brini AT, D'Amico G, Fagioli F, Ferrero I, Locatelli F, Maccario R, Marazzi M,
Parolini O, Pessina A, Torre ML. Mesenchymal stem/stromal cells: a new "cells
as drugs" paradigm. Efficacy and critical aspects in cell therapy. Curr Pharm
Des. 2012 Dec 26. [Epub ahead of print]
19. Marin V, Cribioli E, Philip B, Tettamanti S, Pizzitola I, Biondi A, Biagi E, Pule M.
Comparison of different suicide gene strategies for the safety improvement of
genetically manipulated T cells. Hum Gene Ther Methods. 2012 Nov 27. [Epub
ahead of print] PubMed PMID: 23186165.
20. Kuçi S, Henschler R, Müller I, Biagi E, Meisel R. Basic biology and clinical
application of multipotent mesenchymal stromal cells: from bench to bedside.
Stem Cells Int. 2012;2012:185943. Epub 2012 Aug 28. PubMed PMID:
4
22969811; PubMed Central PMCID: PMC3434412.
21. Taddio A, Biondi A, Piscianz E, Valencic E, Biagi E, Badolato R. From Bone
Marrow Transplantation to Cellular Therapies: Possible therapeutic strategies in
managing autoimmune disorders. Curr Pharm Des. 2012 Jun 21. [Epub ahead
of print] PubMed PMID: 22726117..
22. Lucchini G, Dander E, Pavan F, Di Ceglie I, Balduzzi A, Perseghin P, Gaipa G,
Algarotti A, Introna M, Rambaldi A, Rovelli A, Biondi A, Biagi E, D'Amico G.
Mesenchymal stromal cells do not increase the risk of viral reactivation nor the
severity of viral events in recipients of allogeneic stem cell transplantation.Stem
Cells Int. 2012;2012:690236. Epub 2012 May 30. PubMed PMID: 22701127;
PubMed Central PMCID: PMC3369546.
23. Dander E, Lucchini G, Vinci P, Introna M, Masciocchi F, Perseghin P, Balduzzi
A, Bonanomi S, Longoni D, Gaipa G, Belotti D, Parma M, Algarotti A, Capelli
C,Golay J, Rovelli A, Rambaldi A, Biondi A, Biagi E, D'Amico G. Mesenchymal
stromal cells for the treatment of graft-versus-host disease: understanding the
in vivo biological effect through patient immune monitoring. Leukemia. 2012
Jul;26(7):1681-4. doi: 10.1038/leu.2011.384. Epub 2012 Jan 13. PubMed
PMID: 22289986.
24. Dutour A, Marin V, Pizzitola I, Valsesia-Wittmann S, Lee D, Yvon E, Finney H,
Lawson A, Brenner M, Biondi A, Biagi E, Rousseau R. In Vitro and In Vivo
Antitumor Effect of Anti-CD33 Chimeric Receptor-Expressing EBV-CTL against
CD33 Acute Myeloid Leukemia. Adv Hematol. 2012;2012:683065. Epub 2012
Jan 5. PubMed PMID: 22272203; PubMed Central PMCID: PMC3261457.
25. Biagi E, Marin V, Attianese GM, Pizzitola I, Tettamanti S, Cribioli E, Biondi A.
New advances in leukaemia immunotherapy by the use of Chimeric Artificial
Antigen Receptors (CARs): state of the art and perspectives for the near future.
Ital J Pediatr. 2011 Sep 22;37:46. Review. PubMed PMID: 21939556; PubMed
Central PMCID: PMC3195094.
26. Okur FV, Yvon E, Biagi E, Dotti G, Carrum G, Heslop H, Mims MP, Fratantoni
JC, Peshwa MV, Li L, Brenner MK. Comparison of two CD40-ligand/interleukin2 vaccines in patients with chronic lymphocytic leukemia. Cytotherapy. 2011
Oct;13(9):1128-39. Epub 2011 Jul 12. PubMed PMID: 21745159.
27. Pizzitola I, Agostoni V, Cribioli E, Pule M, Rousseau R, Finney H, Lawson A,
Biondi A, Biagi E, Marin V. In vitro comparison of three different chimeric
receptor-modified effector T-cell populations for leukemia cell therapy. J
Immunother. 2011 Jul-Aug;34(6):469-79. PubMed PMID: 21654519.
28. Giordano Attianese GM, Marin V, Hoyos V, Savoldo B, Pizzitola I, Tettamanti S,
5
Agostoni V, Parma M, Ponzoni M, Bertilaccio MT, Ghia P, Biondi A, Dotti G,
Biagi E. In vitro and in vivo model of a novel immunotherapy approach for
chronic lymphocytic leukemia by anti-CD23 chimeric antigen receptor. Blood.
2011 May5;117(18):4736-45. Epub 2011 Mar 15. PubMed PMID: 21406718;
PubMed Central PMCID: PMC3100686.
29. Balduzzi A, Lucchini G, Hirsch HH, Basso S, Cioni M, Rovelli A, Zincone A,
Grimaldi M, Corti P, Bonanomi S, Biondi A, Locatelli F, Biagi E, Comoli P.
Polyomavirus
JC-targeted
T-cell
therapy
for
progressive
multiple
leukoencephalopathy in a hematopoietic cell transplantation recipient. Bone
Marrow Transplant. 2011 Jul;46(7):987-92. doi: 10.1038/bmt.2010.221. Epub
2010 Oct 4. PubMed PMID: 20921942.
30. Marin V, Pizzitola I, Agostoni V, Attianese GM, Finney H, Lawson A, Pule M,
Rousseau R, Biondi A, Biagi E. Cytokine-induced killer cells for cell therapy of
acute myeloid leukemia: improvement of their immune activity by expression of
CD33-specific chimeric receptors. Haematologica. 2010 Dec;95(12):2144-52.
Epub 2010 Aug 16. PubMed PMID: 20713459; PubMed Central PMCID:
PMC2995574.
31. Laurin D, Marin V, Biagi E, Pizzitola I, Agostoni V, Gallot G, Vié H, Jacob MC,
Chaperot L, Aspord C, Plumas J. Exploration of the lysis mechanisms of
leukaemic blasts by chimaeric T-cells. J Biomed Biotechnol. 2010;2010:234540.
Epub 2010 Jun 13. PubMed PMID: 20617141; PubMed Central PMCID:
PMC2896659.
32. Lucchini G, Introna M, Dander E, Rovelli A, Balduzzi A, Bonanomi S, Salvadè
A, Capelli C, Belotti D, Gaipa G, Perseghin P, Vinci P, Lanino E, Chiusolo P,
Orofino MG, Marktel S, Golay J, Rambaldi A, Biondi A, D'Amico G, Biagi E.
Platelet-lysate-expanded mesenchymal stromal cells as a salvage therapy for
severe resistant graft-versus-host disease in a pediatric population. Biol Blood
Marrow Transplant. 2010 Sep;16(9):1293-301. Epub 2010 Mar 27. PubMed
PMID: 20350611.
33. Foster AE, Okur FV, Biagi E, Lu A, Dotti G, Yvon E, Savoldo B, Carrum G,
Goodell MA, Heslop HE, Brenner MK. Selective elimination of a chemoresistant
side population of B-CLL cells by cytotoxic T lymphocytes in subjects receiving
an autologous hCD40L/IL-2 tumor vaccine. Leukemia. 2010 Mar;24(3):563-72.
Epub 2010 Jan 14. PubMed PMID: 20072155; PubMed Central PMCID:
PMC2836398.
34. Dander E, Balduzzi A, Zappa G, Lucchini G, Perseghin P, Andrè V, Todisco E,
Rahal D, Migliavacca M, Longoni D, Solinas G, Villa A, Berti E, Mina PD, Parma
6
M, Allavena P, Biagi E, Rovelli A, Biondi A, D'Amico G. Interleukin-17producing T-helper cells as new potential player mediating graft-versus-host
disease
in
patients
undergoing
allogeneic
stem-cell
transplantation.
Transplantation. 2009 Dec 15;88(11):1261-72. PubMed PMID: 19996925.
35. Foster AE, Okur FV, Biagi E, Lu A, Dotti G, Yvon E, Savoldo B, Carrum G,
Andreeff M, Goodell MA, Heslop HE, Brenner MK. Selective depletion of a
minor subpopulation of B-chronic lymphocytic leukemia cells is followed by a
delayed but progressive loss of bulk tumor cells and disease regression. Mol
Cancer. 2009 Nov 18;8:106. PubMed PMID: 19922650; PubMed Central
PMCID: PMC2784756.
36. Beretta C, Leoni V, Rossi MR, Jankovic M, Patroniti N, Foti G, Biagi E.
Prolonged extracorporeal membrane oxygenation therapy for severe acute
respiratory distress syndrome in a child affected by rituximab-resistant
autoimmune hemolytic anemia: a case report. J Med Case Rep. 2009 Apr
1;3:6443. PubMed PMID: 19830103; PubMed Central PMCID: PMC2726476.
37. Gaipa G, Tilenni M, Straino S, Burba I, Zaccagnini G, Belotti D, Biagi E,
Valentini M, Perseghin P, Parma M, Campli CD, Biondi A, Capogrossi MC,
Pompilio G, Pesce M. GMP-based CD133(+) cells isolation maintains
progenitor
angiogenic
properties
and
enhances
standardization
in
cardiovascular cell therapy. J Cell Mol Med. 2010 Jun;14(6B):1619-34. Epub
2009 Jul 20. PubMed PMID: 19627397.
38. Salvadè A, Della Mina P, Gaddi D, Gatto F, Villa A, Bigoni M, Perseghin P,
Serafini M, Zatti G, Biondi A, Biagi E. Characterization of platelet lysate
cultured mesenchymal stromal cells and their potential use in tissue-engineered
osteogenic devices for the treatment of bone defects. Tissue Eng Part C
Methods. 2010 Apr;16(2):201-14. PubMed PMID: 19469694.
39. Capelli C, Salvade A, Pedrini O, Barbui V, Gotti E, Borleri G, Cabiati B, Belotti
D, Perseghin P, Bellavita P, Biondi A, Biagi E, Rambaldi A, Golay J, Introna M.
The washouts of discarded bone marrow collection bags and filters are a very
abundant source of hMSCs. Cytotherapy. 2009;11(4):403-13. PubMed
PMID:19462317.
40. Di Biaso I, Di Maio L, Bugarin C, Gaipa G, Dander E, Balduzzi A, Parma M,
D'Amico G, Perseghin P, Biondi A, Biagi E. Regulatory T cells and
extracorporeal photochemotherapy: correlation with clinical response and
decreased frequency of proinflammatory T cells. Transplantation. 2009 May
15;87(9):1422-5. PubMed PMID: 19424046.
41. Lucchini G, Masera N, Foti G, Assali G, Perseghin P, Biagi E. Alife-threatening
7
paediatric case of acute autoimmune haemolytic anaemia (AIHA) successfully
cured by plasma-exchange and combined immunosuppressive treatment.
Transfus Apher Sci. 2009 Apr;40(2):115-8. Epub 2009 Feb 23. PubMed PMID:
19237316.
42. Biagi E, Col M, Migliavacca M, Dell'Oro M, Silvestri D, Montanelli A, Peri G,
Mantovani A, Biondi A, Rossi MR. PTX3 as a potential novel tool for the
diagnosis and monitoring of pulmonary fungal infections in immunocompromised
pediatric
patients.
J
Pediatr
Hematol
Oncol.
2008
Dec;30(12):881-5. PubMed PMID: 19131771.
43. Russell HV, Strother D, Mei Z, Rill D, Popek E, Biagi E, Yvon E, Brenner M,
Rousseau R. A phase 1/2 study of autologous neuroblastoma tumor cells
genetically modified to secrete IL-2 in patients with high-risk neuroblastoma. J
Immunother. 2008 Nov-Dec;31(9):812-9. PubMed PMID: 18833006.
44. Perseghin P, D'Amico G, Dander E, Gaipa G, Dassi M, Biagi E, Biondi A.
Isolation of monocytes from leukapheretic products for large-scale GMPgradegeneration of cytomegalovirus-specific T-cell lines by means of an
automated elutriation device. Transfusion. 2008 Aug;48(8):1644-9. Epub 2008
May 29. PubMed PMID: 18513258.
45. Dander E, Li Pira G, Biagi E, Perseghin P, Renoldi G, Gaipa G, Introna M,
Marin V, Manca F, Biondi A, D'Amico G. Characterization of migratory activity
and cytokine profile of helper and cytotoxic CMV-specific T-cell lines expanded
by a selective peptide library. Exp Hematol. 2008 Apr;36(4):473-85. Epub 2008
Feb 8. PubMed PMID: 18261836.
46. Salvadè A, Belotti D, Donzelli E, D'Amico G, Gaipa G, Renoldi G, Carini F,
Baldoni M, Pogliani EM, Tredici G, Biondi A, Biagi E. GMP-grade preparation of
biomimetic scaffolds with osteo-differentiated autologous mesenchymal stromal
cells for the treatment of alveolar bone resorption in periodontal disease.
Cytotherapy. 2007;9(5):427-38. PubMed PMID: 17786604.
47. Biagi E, Di Biaso I, Leoni V, Gaipa G, Rossi V, Bugarin C, Renoldi G, Parma M,
Balduzzi A, Perseghin P, Biondi A. Extracorporeal photochemotherapy is
accompanied
by
increasing
levels
of
circulating
CD4+CD25+GITR+Foxp3+CD62L+ functional regulatory T-cells in patients with
graft-versus-host disease. Transplantation. 2007 Jul 15;84(1):31-9. PubMed
PMID: 17627234.
48. Introna M, Borleri G, Conti E, Franceschetti M, Barbui AM, Broady R, Dander E,
Gaipa G, D'Amico G, Biagi E, Parma M, Pogliani EM, Spinelli O, Baronciani D,
Grassi A, Golay J, Barbui T, Biondi A, Rambaldi A. Repeated infusions of
8
donor-derived cytokine-induced killer cells in patients relapsing after allogeneic
stem cell transplantation: a phase I study. Haematologica. 2007 Jul;92(7):9529. PubMed PMID: 17606446.
49. Russell HV, Strother D, Mei Z, Rill D, Popek E, Biagi E, Yvon E, Brenner M,
Rousseau R. Phase I trial of vaccination with autologous neuroblastoma tumor
cells genetically modified to secrete IL-2 and lymphotactin. J Immunother. 2007
Feb-Mar;30(2):227-33. PubMed PMID: 17471169.
50. Biagi E, Marin V, Giordano Attianese GM, Dander E, D'Amico G, Biondi A.
Chimeric T-cell receptors: new challenges for targeted immunotherapy in
hematologic malignancies. Haematologica. 2007 Mar;92(3):381-8. Review.
PubMed PMID: 17339188.
51. D'Amico G, Bonamino M, Dander E, Marin V, Basso G, Balduzzi A, Biagi E,
Biondi A. T cells stimulated by CD40L positive leukemic blasts-pulsed dendritic
cells meet optimal functional requirements for adoptive T-cell therapy.
Leukemia. 2006 Nov;20(11):2015-24. Epub 2006 Sep 21. PubMed PMID:
16990769.
52. Marin V, Dander E, Biagi E, Introna M, Fazio G, Biondi A, D'Amico G.
Characterization of in vitro migratory properties of anti-CD19 chimeric receptorredirected CIK cells for their potential use in B-ALL immunotherapy. Exp
Hematol. 2006 Sep;34(9):1219-29. PubMed PMID: 16939815.
53. Vera J, Savoldo B, Vigouroux S, Biagi E, Pule M, Rossig C, Wu J, Heslop HE,
Rooney CM, Brenner MK, Dotti G. T lymphocytes redirected against the kappa
light chain of human immunoglobulin efficiently kill mature B lymphocytederived malignant cells. Blood. 2006 Dec 1;108(12):3890-7. Epub 2006 Aug 22.
PubMed PMID: 16926291; PubMed Central PMCID: PMC1895462.
54. Rousseau RF, Biagi E, Dutour A, Yvon ES, Brown MP, Lin T, Mei Z, Grilley B,
Popek E, Heslop HE, Gee AP, Krance RA, Popat U, Carrum G, Margolin JF,
Brenner MK. Immunotherapy of high-risk acute leukemia with a recipient
(autologous) vaccine expressing transgenic human CD40L and IL-2 after
chemotherapy and allogeneic stem cell transplantation. Blood. 2006 Feb
15;107(4):1332-41. Epub 2005 Oct 25. PubMed PMID: 16249392; PubMed
Central PMCID: PMC1895421.
55. Biagi E, Rousseau R, Yvon E, Schwartz M, Dotti G, Foster A, Havlik-Cooper D,
Grilley B, Gee A, Baker K, Carrum G, Rice L, Andreeff M, Popat U, Brenner M.
Responses to human CD40 ligand/human interleukin-2 autologous cell vaccine
in patients with B-cell chronic lymphocytic leukemia. Clin Cancer Res. 2005 Oct
1;11(19 Pt 1):6916-23. PubMed PMID: 16203783.
9
56. Li LH, Biagi E, Allen C, Shivakumar R, Weiss JM, Feller S, Yvon E, Fratantoni
JC, Liu LN. Rapid and efficient nonviral gene delivery of CD154 to primary
chronic lymphocytic leukemia cells. Cancer Gene Ther. 2006 Feb;13(2):215-24.
PubMed PMID: 16082377.
57. Dotti G, Savoldo B, Pule M, Straathof KC, Biagi E, Yvon E, Vigouroux S,
Brenner MK, Rooney CM. Human cytotoxic T lymphocytes with reduced
sensitivity to Fas-induced apoptosis. Blood. 2005 Jun 15;105(12):4677-84.
Epub 2005 Feb 15. PubMed PMID: 15713795; PubMed Central PMCID:
PMC1895003.
58. Biagi E, Dotti G, Yvon E, Lee E, Pule M, Vigouroux S, Gottschalk S, Popat U,
Rousseau R, Brenner M. Molecular transfer of CD40 and OX40 ligands to
leukemic human B cells induces expansion of autologous tumor-reactive
cytotoxic T lymphocytes. Blood. 2005 Mar 15;105(6):2436-42. Epub 2004 Nov
9. PubMed PMID: 15536147.
59. Vigouroux S, Yvon E, Biagi E, Brenner MK. Antigen-induced regulatory T cells.
Blood. 2004 Jul 1;104(1):26-33. Epub 2004 Mar 16. Review. PubMed PMID:
15026316.
60. D'Amico G, Vulcano M, Bugarin C, Bianchi G, Pirovano G, Bonamino M, Marin
V, Allavena P, Biagi E, Biondi A. CD40 activation of BCP-ALL cells generates
IL-10-producing, IL-12-defective APCs that induce allogeneic T-cell anergy.
Blood. 2004 Aug 1;104(3):744-51. Epub 2004 Mar 4. PubMed PMID: 15001471.
61. Vigouroux S, Yvon E, Wagner HJ, Biagi E, Dotti G, Sili U, Lira C, Rooney CM,
Brenner MK. Induction of antigen-specific regulatory T cells following
overexpression of a Notch ligand by human B lymphocytes. J Virol. 2003
Oct;77(20):10872-80. PubMed PMID: 14512537; PubMed Central PMCID:
PMC224961.
62. Yvon ES, Vigouroux S, Rousseau RF, Biagi E, Amrolia P, Dotti G, Wagner HJ,
Brenner MK. Overexpression of the Notch ligand, Jagged-1, induces
alloantigen-specific
human
regulatory
T
cells.
Blood.
2003
Nov
15;102(10):3815-21. Epub 2003 Jul 3. PubMed PMID: 12842995.
63. Rousseau R, Biagi E, Bollard C, Brenner M. [Gene therapy of childhood
cancers: current status and perspectives]. Bull Cancer. 2003 Mar;90(3):227-38.
Review. French. PubMed PMID: 12801825.
64. Biagi E, Yvon E, Dotti G, Amrolia PJ, Takahashi S, Popat U, Marini F, Andreeff
M, Brenner MK, Rousseau RF. Bystander transfer of functional human CD40
ligand from gene-modified fibroblasts to B-chronic lymphocytic leukemia cells.
Hum Gene Ther. 2003 Apr 10;14(6):545-59. PubMed PMID: 12718765.
10
65. Biagi E, Bollard C, Rousseau R, Brenner M. Gene Therapy for Pediatric
Cancer:State of the Art and Future Perspectives. J Biomed Biotechnol.
2003;2003(1):13-24. PubMed PMID: 12686719; PubMed Central PMCID:
PMC179759.
66. Biagi E, Rousseau RF, Yvon E, Vigouroux S, Dotti G, Brenner MK. Cancer
vaccines: dream, reality, or nightmare? Clin Exp Med. 2002 Nov;2(3):109-18.
Review. PubMed PMID: 12463196.
67. Todisco E, Gaipa G, Biagi E, Bonamino M, Gramigna R, Introna M, Biondi A.
CD40 ligand-stimulated B cell precursor leukemic cells elicit interferon-gamma
production by autologous bone marrow T cells in childhood acute lymphoblastic
leukemia. Leukemia. 2002 Oct;16(10):2046-54. PubMed PMID: 12357356.
68. Bonanomi S, Balduzzi A, Tagliabue A, Biagi E, Rovelli A, Corti P, Crippa D,
Uderzo C. Bath PUVA therapy in pediatric patients with drug-resistant
cutaneous
graft-versus-host
disease.
Bone
Marrow
Transplant.
2001
Sep;28(6):631-2. PubMed PMID: 11607781.
69. Biagi E, Rovelli A, De Lorenzo P, Uderzo C. Autologous hematopoietic stem
cell transplantation (AHSCT) as consolidation therapy for childhood acute
myelogenous leukemia in 1st complete remission. Pediatr Hematol Oncol. 2001
Jul-Aug;18(5):359-62. PubMed PMID: 11452409.
70. Ruggiero A, Conter V, Milani M, Biagi E, Lazzareschi I, Sparano P, Riccardi R.
Intrathecal chemotherapy with antineoplastic agents in children. Paediatr Drugs.
2001;3(4):237-46. Review. PubMed PMID: 11354696.
71. Biagi E, Rovelli A, Balduzzi A, De Lorenzo P, Tagliabue A, Uderzo C. TBI,
etoposide and cyclophosphamide as a promising conditioning regimen for BMT
in childhood ALL in second remission. Bone Marrow Transplant. 2000
Dec;26(11):1260-2. PubMed PMID: 11149746.
72. Biagi E, Bambacioni F, Gaipa G, Casati C, Golay J, Biondi A, Introna M.
Efficient lentiviral transduction of primary human acute myelogenous and
lymphoblastic leukemia cells. Haematologica. 2001 Jan;86(1):13-6. PubMed
PMID: 11146564.
73. Uderzo C, Biagi E, Rovelli A, Balduzzi A, Schirò R, Longoni D, Arrigo C,
Nicolini B, Placa L, Da Prada A, Mascaretti L, Giltri G, Galimberti S, Valsecchi
MG, Locasciulli A, Masera G. Bone marrow transplantation for childhood
hematological disorders: a global pediatric approach in a twelve year single
center experience. Pediatr Med Chir. 2000 Jul-Aug;21(4):157-63. PubMed
PMID: 10767974.
74. Biagi E, Perseghin P, Buscemi F, Dassi M, Rovelli A, Balduzzi A. Effectiveness
11
of extracorporeal photochemotherapy in treating refractory chronic graft-versushost
disease.
Haematologica.
2000
Mar;85(3):329-30.
PubMed
PMID:
10702832.
75. Biagi E, Assali G, Rossi F, Jankovic M, Nicolini B, Balduzzi A. A persistent
severe autoimmune hemolytic anemia despite apparent direct antiglobulin test
negativization. Haematologica. 1999 Nov;84(11):1043-5. PubMed PMID:
10553166.
76. Uderzo C, Fraschini D, Balduzzi A, Galimberti S, Arrigo C, Biagi E, Pignanelli
M, Nicolini B, Rovelli A. Long-term effects of bone marrow transplantation on
dental status in children with leukaemia. Bone Marrow Transplant. 1997
Nov;20(10):865-9. PubMed PMID: 9404928.
77. Marchi P, Uderzo C, Riva A, Rovelli A, Biagi E, Arrigo C, Marraro G, Masera G.
Role
of
early
thromboembolism
diagnosis
in
for
leukemic
a
noninvasive
children.
treatment
Support
Care
of
pulmonary
Cancer.
1997
Sep;5(5):417-20. PubMed PMID: 9322356.
78. Dell'Orto M, Rovelli A, Barzaghi A, Valsecchi MG, Silvestri D, Giltri G, Balduzzi
A, Biagi E, Arrigo C, Rossi MR, Masera G, Uderzo C. Febbrile complications in
the first 100 days after bone marrow transplantation in children: a single
center's experience. Pediatr Hematol Oncol. 1997 Jul-Aug;14(4):335-47.
PubMed PMID: 9211538.
79. Biagi E, Arrigo C, Dell'Orto MG, Balduzzi A, Pezzini C, Rovelli A, Masera G,
Silvestri D, Uderzo C. Mechanical and infective central venous catheter-related
complications: a prospective non-randomized study using Hickman and
Groshong catheters in children with hematological malignancies. Support Care
Cancer. 1997 May;5(3):228-33. PubMed PMID: 9176970.
80. Uderzo C, Valsecchi MG, Balduzzi A, Rovelli A, Dini G, Miniero R, Locatelli F,
Rondelli R, Arcese W, Andolina M, Messina C, Polchi P, Biagi E, Arrigo C,
Silvestri D, Masera G, Bacigalupo A. Treatment of childhood acute
lymphoblastic leukemia in first remission with allogeneic bone marrow
transplantation or with intensive chemotherapy: a cooperative Italian study. The
AIEOP (Associazione Italiana Ematologia ed Oncologia Pediatrica) and GITMO
(Gruppo Italiano Trapianto di Midollo Osseo), Italy. Bone Marrow Transplant.
1996 Nov;18 Suppl 2:25-7. PubMed PMID: 8932793.
12
Research experience
- November 1999 - May 2000: “Centro di Ricerche Farmacologiche M.Negri”
(Milan), Laboratory of Immunohematology (Dr. M.Introna).
Project: gene transfer protocol in freshly isolated human AML and B-precursor
ALL cells, using a defective lentivirus derived from the HIV-1 backbone and
carrying the EGFP (enhanced green fluorescent protein) gene (Naldini L,
Nature Biotech 15: 871, 1997).
- November 2000 - June 2001: “Centro di Ricerca M.Tettamanti”, S.Gerardo
Hospital, Monza (Milan), Prof. A.Biondi.
Project: evaluation of the capacity of soluble trimeric CD40 ligand of inducing
the maturation of ALL blasts into mature dendritic cells during culture on human
bone marrow stroma; gene transduction of ALL blasts by means of defective
lentivirus (derived from the HIV-1 backbone) with the gene encoding for the
human CD40 ligand molecule.
- July 2001- December 2004: postdoctoral fellow at “Center for Cell and Gene
Therapy”, Texas Children Hospital, Baylor College of Medicine, Houston
(Texas) – Director: Prof. MK Brenner.
Project: protocol of immunotherapy of patients affected by Chronic Lymphocytic
Leukemia by means of injection of autologous manipulated leukemic cells,
expressing high levels of endogenous CD40 ligand and genetically manipulated
to secrete recombinant human IL-2.
Society membership:
- Associazione Italiana di Emato-Oncologia Pediatrica (AIEOP)
- Societa` Italiana di Pediatria (SIP)
- Società Italiana di Ematologia Sperimentale (SIES)
- Società Italiana di Ricerca Pediatrica (SIRP)
- Società Europea di Ematologia (ESH)
- American Society of Cancer Research (AACR)
- American Society of Hematology (ASH)
Main Grants:
13
-
Progetto
Staminali
Regione
Lombardia
(2005-2007):
“Use
of
mesenchymal stem cells for bone regeneration”.
-
STREP 2006 (6th framework): “Chimaeric T cells for the treatment of
paediatric cancers (Childhope).” See: www.childhope.eu.
-
Progetto Integrato Oncologia-Ministero della Salute 2006: “New tools of
immunotherapy for pediatric cancers”.
-
AIRC 2007: “The use of chimeric T-cell receptors (ChTCRs) for the
therapy of haematological high-risk diseases”.
-
Progetto Sangue regione Lombardia (2007-2008): “Regulatory T cells as
mediators for the effect of extracorporeal photochemotherapy for the therapy of
graft-versus-host-disease”.
-
Progetto
Sangue
regione
Lombardia
(2008-2009):
“PRP
and
mesenchymal stem cells combined with synthetic scaffolds for the cure of bone
degenerative diseases”.
-
Personal prize (Regione Lombardia), December 2008: best young
researcher for innovative treatment by the establishment of international
networks (“Progetto Ricercatissimi”).
-
Grant
JUST
“Libera
le
ali”
(2011):
http://www.fondazionejustitalia.org/progetti/libera-le-ali.aspx.
-
Progetto
mesenchymal
Staminali
stem
cells
Regione
for
Lombardia
GvHD
(2011-2012):
treatment
after
bone
“Use
of
marrow
transplantation”.
-
AIRC 2011 Molecular clinical oncology 5 per mille, "Innate immunity in
cancer. Molecular targeting and cellular therapy".
-
IG AIRC grant 2015: Novel leukemia treatment by the use of Chimeric
Antigen Receptors (CARs). Project Code: 17248
14