curriculum vitae stefania piersanti personal information education

CURRICULUM VITAE
STEFANIA PIERSANTI
PERSONAL INFORMATION
Name: Stefania Piersanti
Nationality: Italian
Languages: Italian, English
Work address: Department of Biology and Biotechnology "C. Darwin", University of Rome “La Sapienza”, ex Institute
of Genetics, p.le Aldo Moro, 5, 00185, Rome, Italy
Work Phone number: +390649912472
E-mail: [email protected]
EDUCATION AND TRAINING
2005: PhD in Genetics and Molecular Biology, Department of Genetics and Molecular Biology, University of Rome “La
Sapienza”. PhD Title of thesis: ”Adenovirus-integrin interaction. Molecular characterization and functional exploitation
for the production of a new gene transfer vector”. Group Leader: Prof. Isabella Saggio
2000: Master Degree in Biology . Score 110/110 cum laude
Dept. of Genetics and Molecular Biology, University “La Sapienza”, Rome. Title of thesis: “ Library of mutants of
Adenoviral Penton base. Construction, characterization and applications”. Group Leader: Prof. Isabella Saggio
PROFESSIONAL, TEACHING EXPERIENCES AND AWARDS
2013 SEPT-OCT.: Post-doctoral Marie Curie fellow, Industry-Academia Partnerships and Pathways (IAPP), Marie Curie
Actions (People, FP7)
2012: Meta-Analysis course, 4-8 June 2012, Cesenatico (FC)
2010-today: Temporary research contracts and post-doc activity in the field of gene therapy and viral vectors at the
University of Rome La Sapienza, Department of Biology and Biotechnology.
2010: Visiting scientist in IBET, Oeiras, Lisbon, Portugal. Reasearch activity in the field of 3D neuronal progenitor cells.
2010: Travelling grant award of Journal of Cell Science and The Company of Biologists Limited
2009: Visiting scientist in CNRS, Eric Kremer lab, Montpellier, France. Research activity in the field of canine
adenovectors
2006: Registeredin the National Council of Biologists (Albo professionale dei biologi), Section A.
2006:ECTS Young Investigator Award at the 33rd European Symposium on Calcified Tissues, 10-14 May 2006, Prague,
Czech Republic, based on the submission of the abstract “Transfer and silencing of a disease gene (R201C-GNAS) in
human Skeletal Stem Cells (SSCs)”
2005-2008: Temporary professor in Genetics at the University of Urbino “Carlo Bo”.
2005-2009: Telethon research contract and post-doc activity at the Scientific Park of Rome “San Raffaele”, Institute of
Tissue engineering and cellular biology, Rome. Group leaders, Prof Isabella Saggio and Prof Paolo Bianco
2003: Stage in Flow Cytometry techniques at the “Center of Excellence in Molecular Medicine (MolMed)”, Lodz
(Poland) and research activity in the field of apoptosis of tumoral cells in hematological diseases. Group leaders Dr.
Piotr Smolewski and Prof. Czeslaw Cierniewski.
2001: Research fellowship of Pasteur Institute- Fondazione Cenci Bolognetti of Rome, in the field of virus-cell
interaction. Group leader Prof I. Saggio.
2000: Post-lauream training at the Department of Genetics and Molecular Biology, University of Rome La Sapienza, in
the field of phage display technology.
TECHNICAL SKILLS AND COMPETENCES
Protein Extraction , Western Blotting- Lentiviral vector preparation, titration and transduction– Adenoviral vectors
preparation, titration and transduction- M13/Lambda Phage display technology -Cell cultures of primary, stem cells
and cell lines. Differentiation of stem cells-Cell Transfection -Bacterial Transformation -Microbioly Tecniques - RT-PCR Real Time PCR - RNA extraction from cells and tissues and retrotranscription - Cloning Techniques - Plasmid DNA
Purification (Maxiprep, Midiprep, Miniprep) -Genomic DNA isolation from cells and tissues -Cytological Preparation
and analysis- Immunoflurescence- Cytofluorimetry analysis- ELISA- enzyme assays.
COMPUTER KNOWLEDGE -Microsoft Office package - GeneAmp7300 - Adobe Photoshop CS4- WinMDI- Cell Quest- Scion
Image- DNAMan- Bioconductor microarray package- Ingenuity Pathway Analysis software- TM4-MeV-Gene-E- Smart
Draw- MiniTab 13- Sigma Plot- Cytoscape 2.8.3-MetaMorph image analysis software-EndNote9- GenoPro- EASEPrimer Express
PUBLICATIONS (IN EXTENSO)
D. Simão, C. Pinto, S. Piersanti, A. Weston, L. Collinson, S. Salinas, M. Serra, A. P. Teixeira, I. Saggio, E. J. Kremer, G.
Schiavo, C. Brito, P. M. Alves. Modeling human neural functionality in vitro: 3D culture for dopaminergic
differentiation, subitted to Nature Methods.
Piersanti S, Tagliafico E, Saggio I. DNA microarray to analyse Adenovirus-host interactions. Methods in Molecular
Biology , 2014; 1089:89-104.
Piersanti S, Astrologo L, Licursi V, CostaR, Roncaglia E, Gennetier A, Ibanes S, Chillon M, Negri R, Tagliafico, Kremer EJ,
Saggio I. Differentiated neuroprogenitor cells incubated with human or canine adenovirus, or lentiviral vectors
have distinct transcriptome profiles. PLoS One. 2013 Jul 26;8(7):e69808. doi: 10.1371
Piersanti S, Remoli C, Saggio I, Funari A, Michienzi S, et al. Transfer, Analysis, and Reversion of the Fibrous Dysplasia
Cellular Phenotype in Human Skeletal Progenitors. Journal of Bone and Mineral Research, 2010, 25: 1103-1116.
Caruso P, Burla R, Piersanti S, Cherubini G, Remoli C, et al. Prion expression is activated by Adenovirus 5 infection and
affects the adenoviral cycle in human cells. Virology, 2009, 385: 343-350.
Sacchetti B, Funari A, Michienzi S, Di Cesare S, PiersantiS, et al. Self-renewing osteoprogenitors in bone marrow
sinusoids can organize a hematopoietic microenvironment. Cell, 2007,131: 324-336.
Martina Y, Avitabile D, Piersanti S, Cherubini G, Saggio I. Different modulation of cellular transcription by adenovirus 5,
Delta E1/E3 adenovirus and helper-dependent vectors. Virus Research 2007, 130: 71-84.
Piersanti S, Sacchetti B, Funari A, Di Cesare S, Bonci D, et al. Lentiviral transduction of human postnatal skeletal
(stromal, mesenchymal) stem cells: In vivo transplantation and gene silencing. Calcified Tissue International 2006,
78: 372-384.
Cherubini G, Petouchoff T, Grossi M, Piersanti S, Cundari E, et al. E1B55K-deleted adenovirus (ONYX-015) overrides
G(1)/S and G(2)/M checkpoints and causes mitotic catastrophe and endoreduplication in p53-proficient normal
cells. Cell Cycle, 2006, 5: 2244-2252.
Piersanti S, Cherubini G, Martina Y, Salone B, Avitabile D, et al. Mammalian cell transduction and internalization
properties of lambda phages displaying the full-length adenoviral penton base or its central domain. Journal of
Molecular Medicine-Jmm, 2004, 82: 467-476.
Piersanti S, Martina Y, Cherubini G, Avitabile D, Saggio I. Use of DNA microarrays to monitor host response to virus
and virus-derived gene therapy vectors. Am J Pharmacogenomics, 2004, 4: 345-356.
Salone B, Martina Y, Piersanti S, Cundari E, Cherubini G, et al. Integrin alpha 3 beta 1 is an alternative cellular receptor
for adenovirus serotype 5. Journal of Virology, 2003, 77: 13448-13454.
CONGRESS PROCEEDINGS
S. Piersanti; L. Astrologo; V. Licursi; R. Negri; E. Roncaglia; E. Tagliafico; E.J. Kremer; I. Saggio (2012), [511797],Canine
adenovirus (CAV-2) vectors induce an innate immune response and the modulation of cell cycle genes in
dopaminergic differentiated human midbrain neuronal precursors, Human Gene Therapy,Mary Ann Liebert inc,
140 Huguenot Street, 3rd FL, New Rochelle, NY 10801 UMilan, Italy
Piersanti S, Astrologo L, Schwarz SC, Schwarz J, Chillon M, Kremer E, Saggio I (2010), [462111], "Highthroughput
transcriptional analysis of gene therapy viral vectors effects on brain cells.Human Gene Therapy, 21(10): 14261426
Michienzi S, Piersanti S, Funari A, Remoli C, Cersosimo S, Costa R, Saggio I, Bianco P, Riminucci M (2009). [462147],
"Phosphodiesterase-mediated adaptation to Gs-alpha mutations is developmentally regulated in embryonic
and post-natal stem cells." Bone, 44: 435
Martina Y, Avitabile D, Piersanti S, Cherubini G, Salone B, Saggio I (2003). [462171]."Gene expression is altered by
infection with adenovirus and adeno-derived vectors. Global monitoring with oligonucleotide arrays."
Molecular Therapy, 7(5): 11.
ARTICLES IN BOOKS
Piersanti S, “RNA: altro che messaggero!”, Annual book, Updated supplement of the “Grande Enciclopedia Medica
Curcio”2009- Curcio Editore
Piersanti S,“Cellule staminali ringiovanite”, Annual book, Updated supplement of the “Grande Enciclopedia Medica
Curcio”2010, Curcio Editore
Piersanti S, “Cellule sintetiche:create o copiate?”, Annual book, Updated supplement of the “Grande Enciclopedia
Medica Curcio”2011, Curcio Editore
Piersanti S, “Virus nella terapia del cancro”, Annual book, Updated supplement of the “Grande Enciclopedia Medica
Curcio”2012, Curcio Editore
Piersanti S, “Terapia genica per le malattie del cervello”, Annual book, Updated supplement of the “Grande
Enciclopedia Medica Curcio”2013, Curcio Editore
CONGRESSPARTICIPATION
-M.Di Giovine, B. Salone, Y. Martina, V. Amati, S. Piersanti, V.Lanza,C.M. Failla, I.Saggio. Studio della base pentonica di
o
adenovirus mediante l'impiego della tecnologia fagica. Presentazione poster, 1 Convegno FISV, Riva del Garda (TN),
October 1999.
-B.Salone, K. Benihoud,S. Esselin, M.Di Giovine, Y. Martina, S. Piersanti, I.Saggio. Studio sull'inibizione delle citochine
infiammatorie per il miglioramento del trasferimento genico mediante vettori adenovirali. Presentazione poster, 1
o
Convegno FISV, Riva del Garda (TN), Ottobre 1999.
-Salone B.; Martina Y.; Di Giovine M.; Piersanti S.; Cherubini G.; Di Zenzo G.; Failla C.M.; Saggio I. Recombinant
bacteriophages as gene delivery vectors for mammalian cells. Oral Comunication CSH, Gene Therapy day 2001
-S. Piersanti, Y. Martina, E. Cundari, B. Salone, G. Cherubini, D. Avitabile, F. Grosso, G. Di Zenzo and I. Saggio.
Engineered lambda bacteriophage for efficient transduction of mammalian cells. Poster presentation, 4° Convegno
FISV, September 2002
-S. Piersanti, Y. Martina, E. Cundari, B. Salone, G. Cherubini, D. Avitabile, F. Grosso, G. Di Zenzo and I. Saggio.
Engineered lambda bacteriophage for efficient transduction of mammalian cells. Oral presentation and poster, VI
Congresso Nazionale Biotecnologie, June 2003
-Stefania Piersanti, Benedetto Sacchetti, Vanessa Velotta, Simonetta Morese, Alessia Funari, Silvia Di Cesare, Desiré
Bonci, Cesare Peschle, Mara Riminucci, Paolo Bianco and Isabella Saggio. Lentiviral mediated transduction of human
skeletal (stromal, mesenchymal) stem cells. Poster FISV, September 2004
-P. Bianco, A. Funari, S. Piersanti, S. Michienzi, I.Saggio, M. Riminucci
Fibrous Dysplasia: models of disease, models of intervention, XIII Convegno Telethon, Poster, March 2005.
-S. Piersanti, B. Sacchetti, A. Funari, C. Remoli, S. Michienzi, M. Riminucci, I. Saggio, P. Bianco. Transfer and silencing of
a disease gene (R201C-GNAS) in human skeletal stem cells (sscs), XXXIII European Symposium on Calcified Tissues
(Prague), Poster and oral communication, May 2006
-S. Michienzi, A. Funari, B. Sacchetti, S. Cersosimo, S. Piersanti, C. Remoli, I. saggio, M. Riminucci and P. Bianco. FGF-2
and TGFβ-1 have opposite effects on the mural cell phenotype of CD146+ BMSCs.Genostem, 3nd Workshop on
Mesenchymal Stem Cells Engineering for Connective Tissue Disorders Poster, Barcellona (Spain) il 7-9 February 2007.
-B. Sacchetti, A. Funari, S. Michienzi, G. Giannicola, S. Cersosimo, S. Piersanti, C. Remoli, I. Saggio, P. Robey, M.
Riminucci and P. Bianco. Prospective isolation of MCAM/CD146-expressing, clonogenic cells endowed with high
spontaneous myogenic activity from the microvasculature of human adult skeletal muscle.Genostem, 3nd Workshop
on Mesenchymal Stem Cells Engineering for Connective Tissue Disorders, Poster, Barcellona (Spain) il 7-9 February
2007.
-A. Funari, S. Michienzi, B. Sacchetti, S. Cersosimo, S. Piersanti, C. Remoli, I. saggio, M. Riminucci and P. Bianco. The
potential role of CD146+ stromal cells in the assembly and remodeling of pseudovascular structures and in the
establishment of HSC niche.Genostem, 3nd Workshop on Mesenchymal Stem Cells Engineering for Connective
Tissue Disorders, Poster, Barcellona (Spain) il 7-9 February 2007.
-P. Bianco, A. Funari, S. Piersanti, S. Michienzi, I. Saggio, M. Riminucci. Fibrous dysplasia, models of disease, models of
intervention. XIV Convention Scientifica Telethon, Poster, Salsomaggiore Terme – Palazzo dei Congressi, 12-14 March
2007.
-Isabella Saggio, Stefania Piersanti, Alessia Funari, Cristina Remoli, Stefania Cersosimo, Benedetto Sacchetti, Stefano
Michienzi, Pamela Gehron Robey, Mara Riminucci, Paolo Bianco. Modeling and Correcting Fibrous Dysplasia with
Lentiviral Vectors. Poster, ASGT 11th Annual Meeting, May 28–June 1, 2008, Boston, Massachusettes (USA).
-S. Piersanti, I. Saggio, A. Funari, C. Remoli, S. Cersosimo, P.G. Robey, M. Riminucci, P. Bianco.Modeling and Correcting
Fibrous Dysplasia with Lentiviral Vectors. FISV 2008, Poster e Oral communication, Riva del Garda, 24-27 September
2008.
-M. Riminucci, I. Saggio, S. Michienzi, C. Remoli, S. Cersosimo, S. Piersanti, A. Di Consiglio, A. Funari, P. Bianco. Fibrous
dysplasia: models of disease, models of intervention.Poster and Oral comunication, XV CONVENTION SCIENTIFICA
TELETHON, Palazzo dei Congressi, Riva del Garda (Trento) 9-11 march 2009
-S.Piersanti, L. Astrologo, S.C. Schwarz, J. Schwarz, M. Chillon, E. Kremer, I. Saggio . High-throughput transcriptional
analysis of gene therapy viral vectors effects on brain cells. Poster, European Society of Gene and Cell Therapy 2010,
(ESGCT) Milano, Italy, 22-25 October 2010.
-Piersanti S., Astrologo L., Licursi V., Negri R., Roncaglia E., Tagliafico E., Schwarz S., Schwarz J., Kremer E.J., Saggio I.
Canine adenovirus (CAV-2) vectors induce an innate immune response and a modulation of cell cycle genes in human
dopaminergic neurons. Poster, 10thInternational Adenovirus Meeting. Umeå, Sweden (June 2012)
-Astrologo L., Piersanti S., Remoli C., Costa R., Tagliafico E., Roncaglia E., Funari A., SacchettiB., Riminucci M., Saggio I.,
Bianco P. High-throughput analysis of downstream effects of activating Gs alpha mutations in skeletal progenitor/stem
cells. Oral communication, FISV 2012, 12th Congress. Rome, Italy (September 2012)
-Piersanti S., Astrologo L., Licursi V., Negri R., Roncaglia E., Tagliafico E., Schwarz S., SchwarzJ., Kremer E.J., Saggio I.
Canine adenovirus (CAV-2) vectors induce an innate immune response and a modulation of cell cycle genes in
dopaminergic differentiated human midbrain neuronal progenitors. Poster, FISV 2012, 12th Congress. Rome, Italy
(September 2012)
-S. Piersanti, L. Astrologo, V. Licursi, R. Negri, E. Roncaglia, E. Tagliafico, E. Kremer and I. Saggio
Canine adenovirus (CAV-2) vectors induce an innate immune response and a modulation of cell cycle genes in
dopaminergic differentiated human midbrain neuronal progenitors. Poster, European Society of Gene and Cell
Therapy 2012, (ESGCT) Versailles, France, 25-29 October 2012.