Cell Biology - The Protein Factory

Unit
Laboratory Website
Cell Biology
http://www.dbsm.uninsubria.it/biocellu/
Leaders
Members
Giovanni Bernardini (Full professor of Citology
Federica Rossi, Laboratory technician
and Histology)
([email protected])
[email protected].
Cristina Pirrone, Research Fellow
Phone +39 0332 421 316
([email protected])
Rosalba Gornati (Associated professor of
Riccardo Balzaretti, PhD student
Citology and Histology)
([email protected])
[email protected].
Silvia Palombella, PhD student
Phone +39 0332 421 314
([email protected])
Ilaria Armenia, PhD student
([email protected])
Academic Structure
Keywords
Dept. of Biotechnology and Life Sciences
1. Nanoparticles
Via J.H. Dunant 3 – 21100 Varese
2. Gene expression
3. Molecular biomarkers
4. Tossicology
Research Topics
Expertise
1. Nanotechnology with particular interest in the 1. Magnetic nanoparticle-system preparation and
development of conjugated nano-systems.
application.
2. Nanotoxicology and nanoparticle cell uptake.
2. Optical and TEM microscopy sample preparation and
3. Search and evaluation of molecular biomarkers analysis.
with particular attention to those involved in cell 3. Molecular Biology basic procedures: DNA, RNA
differentiation.
extraction and purification, PCR and qPCR, image
acquisition, gene cloning.
4. Basic procedures of protein analysis: extraction,
purification, gel electrophoresis and immunoblot.
Recent Publications
1. Elena Bossi, Daniele Zanella, Rosalba Gornati, Giovanni Bernardini. Cobalt oxide nanoparticles can
enter inside the cells by crossing plasma membranes. Scientific Reports 6:22254, 1-9 DOI:
10.1038/srep22254 (2016).
2. Gornati R, Longo A, Rossi F, Maisano M, Sabatino G, Mauceri A, Bernardini G, Fasulo S. Effects of
titanium dioxide nanoparticle exposure in Mytilus galloprovincialis gills and digestive gland.
Nanotoxicology 5:1-11 (2016).
3. Gornati R, Pedretti E, Rossi F, Cappellini F, Zanella M, Olivato I, Sabbioni E, Bernardini G. Zerovalent
Fe, Co and Ni nanoparticle toxicity evaluated on SKOV-3 and U87 cell lines. J Appl Toxicol. 2016. 36:385393 (2016).
4. Sabbioni E, Fortaner S, Farina M, Del Torchio R, Olivato I, Petrarca C, Bernardini G, Mariani-Costantini
R, Perconti S, Di Giampaolo L, Gornati R, Di Gioacchino M. Cytotoxicity and morphological transforming
potential of cobalt nanoparticles, microparticles and ions in Balb/3T3 mouse fibroblasts: an in vitro model.
Nanotoxicology 8:455-464 (2014).
5. Sabbioni E, Fortaner S, Farina M, Del Torchio R, Petrarca C, Bernardini G, Mariani-Costantini R,
Perconti S, Di Giampaolo L, Gornati R, Di Gioacchino M. Interaction with culture medium components,
cellular uptake and intracellular distribution of cobalt nanoparticles, microparticles and ions in Balb/3T3
mouse fibroblasts. Nanotoxicology 8:88-99 (2014).