Curriculum Vitae

Curriculum Vitae
Dr. Francesca Sacco, PhD
Francesca Sacco
PERSONAL INFORMATION
Implerstrasse 72, 81371 Munich (Germany)
+49 176 87603155
[email protected]
https://www.researchgate.net/profile/Francesca_Sacco2
POSITION
Postdoctoral research fellow
WORK EXPERIENCE
Nov 2012–Present
Postdoctoral research fellow in dissecting signaling pathways by
mass spectrometry-based proteomics
Advisor: Matthias Mann
Department of Biochemistry, Max Plank Institute of Biochemistry,
Martinsried (Munich), Germany
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In-depth knowledge of state-of-the-art mass spectrometry (MS),
liquid chromatography (LC) instrumentations and theory related to
this technology.
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Skilled in problem-solve and troubleshoot both MS and LC
instrumentations and sample preparation processes.
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Application of my in-depth knowledge and expertise in MS to
address different long-standing questions in the signal transduction
field and to improve the way to extract biological meaning from MSderived datasets. Specifically:
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I performed a deep proteomic analysis of pancreatic islets
of different genetic diabetic mouse models to characterize
mechanisms leading to beta cells dysfunction and identify
new diabetes markers (collaboration with Sanofi-Aventis).
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I characterized new mechanisms controlling insulin
secretion in pancreatic beta cells (collaboration with
Sanofi-Aventis).
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I identified the molecular mechanisms underlying the anticancer activity of metformin, one of most widely prescribed
diabetic drugs.
I identified the molecular mechanisms leading to
chemotherapeutic resistance in acute myeloid leukemia
cells, in collaboration with the Prof. Thomas Fisher group
(University Hospital of Madgeburg, Magdeburg).
o I designed a novel bioinformatics strategy to map
proteomics and phosphoproteomics dataset onto
literature-derived signaling pathways.
Successfully trained and supervised a full-time technician on all the
technical aspects concerning the MS-sample preparation and raw
data analysis.
Oral presentation of my findings in national and international
conferences/workshops.
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Nov 2011–Oct 2012
Postdoctoral research fellow in elucidating the role of PP2A and
SHP2 phosphatases in cancer pathways by MS-based proteomics.
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Curriculum Vitae
Dr. Francesca Sacco, PhD
Advisor: Gianni Cesareni
Department of Cellular and Molecular biology, University of Rome Tor
Vergata, Rome (Italy)
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Nov 2008–Dec 2011
I identified new substrates and interactors of different members of
the phosphatase family by MS-based proteomics
• I contributed to elucidate the pathways modulated by the oncogene
SHP2 by MS-based phosphoproteomics
PhD student. Advisor: Gianni Cesareni
Department of Cellular and Molecular biology, University of Rome Tor
Vergata, Rome (Italy)
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Aug 2006– Oct 2006
I conceived a novel experimental strategy that enables the mapping
of gene products onto cell specific pathways.
I elucidated the regulatory role of the human phosphatase family
members in pro-proliferative pathways.
I identified a new substrate of the DEP1 oncosoppressor
phosphatase.
I contributed to identify new interactors of SH2 domains containing
proteins.
Visiting research fellow. Advisor: Gustav Ammerer
Max F. Perutz Laboratories, Wien, Austria
EDUCATION AND
TRAINING
15 Dec 2011
15 Jul 2008
15 Jul 2006
Doctor of Philosophy (PhD) in Cellular and Molecular biology
University of Rome Tor Vergata, Rome (Italy)
Master of Science in Cellular and Molecular biology (with honors)
University of Rome Tor Vergata, Rome (Italy)
Bachelor of Science in Cellular and Molecular biology (with honors)
University of Rome Tor Vergata, Rome (Italy)
AREA OF EXPERTISE
• Mass spectrometry-based proteomics,
phosphoproteomics and interactomics
• Systems biology
• Large scale statistical data analysis
• Signal Transduction
• Cancer pathways
• Metabolism
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Beta cells biology
Phosphorylation
Protein-protein interaction
Molecular genetics
Project planning and budgeting
Problem solving
Collaboration establishment
PERSONAL SKILLS
Mother tongue(s)
Other language(s)
English
Italian
UNDERSTANDING
SPEAKING
WRITING
C2
C2
C2
Levels: C2: Proficient user. Common European Framework of
Reference for Languages
ADDITIONAL
INFORMATION
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Curriculum Vitae
Honors and awards
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Dr. Francesca Sacco, PhD
Awarded with the EMBO Long-Term Fellowship from the European
Molecular Biology Organization.
Ad hoc peer reviewer for manuscripts submitted to: Molecular &
Cellular Proteomics, Scientific Reports, FEBS Journal, FEBS
Letters, PLOS one, Oncotarget, Methods, Journal of Biological
chemistry, Frontiers Genetics.
Manuscript selected for the cover image of the March issue 2016 of
the Cell Systems journal.
Poster prize at the “Translating Diabetes” meeting at the EMBL,
Heidelberg, 2014.
Qualification as a Professional Biologist.
Awarded with 5000 euro prize for the highest exam scores obtained
during the bachelor and master degrees.
Conferences
Abstracts selected in 9 international conferences for oral presentations
and 2 international conferences for poster presentations.
Publications
13 scientific publications in peer-reviewed journals
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Curriculum Vitae
Dr. Francesca Sacco, PhD
14. Sacco, F., Humphrey S., Cox, J., Mischnik, M., Klabunde, T., Schafer, M. and Mann, M. Glucoseregulated and drug perturbed beta-cell phosphoproteome reveals molecular mechanisms controlling
insulin secretion. Nature Communications, under review*
I identified new mechanisms controlling insulin secretion by applying MS-based proteomics and
phosphoproteomics.
Submission delayed due to the internal approval procedure of Sanofi-Aventis.
13. Sacco, F., Silvestri, A., Posca, D., Pirrò, S., Gherardini, Pier F., Castagnoli, L., Mann, M., and
Cesareni, G. (2016) Deep Proteomics of Breast Cancer Cells Reveals that Metformin Rewires Signaling
Networks Away from a Pro-growth State. Cell Systems 2, 2, 1-13.
I characterized the mechanisms underlying the metformin action’s by combining MS-based
proteomic datasets with a literature-derived signaling network.
12. Mischnik, M., Sacco, F., Cox, J., Schneider, H.C., Schafer, M., Hendlich, M., Crowther, D., Mann,
M., and Klabunde, T. (2016). IKAP: A heuristic framework for inference of kinase activities from
Phosphoproteomics data. Bioinformatics 32, 424-431.
I contributed to develop a new method to infere kinase activities from phosphoproteomics data.
11. Corallino, S., Iwai, L.K., Payne, L.S., Huang, P.H., Sacco, F., Cesareni, G., and Castagnoli, L.
(2015). Alterations in the phosphoproteomic profile of cells expressing a non-functional form of the
SHP2 phosphatase. New biotechnology.
10. Sacco, F., Boldt, K., Calderone, A., Panni, S., Paoluzi, S., Castagnoli, L., Ueffing, M., and Cesareni,
G. (2014). Combining affinity proteomics and network context to identify new phosphatase substrates
and adapters in growth pathways. Frontiers in genetics 5, 115.
I analyzed MS-based interactomics data to identify new phosphatase substrates and interactors.
9. Tinti, M., Kiemer, L., Costa, S., Miller, M.L., Sacco, F., Olsen, J.V., Carducci, M., Paoluzi, S.,
Langone, F., Workman, C.T., Blom, N., Machida, K., Thompson, C.M., Schutkowski, M., Brunak, S.,
Mann, M., Mayer, B.J., Castagnoli, L., and Cesareni, G. (2013). The SH2 domain interaction landscape.
Cell reports 3, 1293-1305.
8. Liberti, S., Sacco, F., Calderone, A., Perfetto, L., Iannuccelli, M., Panni, S., Santonico, E., Palma, A.,
Nardozza, A.P., Castagnoli, L., and Cesareni, G. (2013). HuPho: the human phosphatase portal. The
FEBS journal 280, 379-387.
7. Sacco, F., Gherardini, P.F., Paoluzi, S., Saez-Rodriguez, J., Helmer-Citterich, M., Ragnini-Wilson, A.,
Castagnoli, L., and Cesareni, G. (2012a). Mapping the human phosphatome on growth pathways.
Molecular systems biology 8, 603.
6. Sacco, F., Perfetto, L., Castagnoli, L., and Cesareni, G. (2012b). The human phosphatase
interactome: An intricate family portrait. FEBS letters 586, 2732-2739.
5. Licata, L., Briganti, L., Peluso, D., Perfetto, L., Iannuccelli, M., Galeota, E., Sacco, F., Palma, A.,
Nardozza, A.P., Santonico, E., Castagnoli, L., and Cesareni, G. (2012). MINT, the molecular interaction
database: 2012 update. Nucleic acids research 40, D857-861.
4. Tinti, M., Nardozza, A.P., Ferrari, E., Sacco, F., Corallino, S., Castagnoli, L., and Cesareni, G. (2012).
The 4G10, pY20 and p-TYR-100 antibody specificity: profiling by peptide microarrays. New
biotechnology 29, 571-577.
3. Sacco, F., Corallino, S., Castagnoli, L. (2011) PTPRJ (protein tyrosine phosphatase, receptor type,
J). AGCOH J Oct; 10: 867-872.
2. Sacco, F., Tinti, M., Palma, A., Ferrari, E., Nardozza, A.P., Hooft van Huijsduijnen, R., Takahashi, T.,
Castagnoli, L., and Cesareni, G. (2009). Tumor suppressor density-enhanced phosphatase-1 (DEP-1)
inhibits the RAS pathway by direct dephosphorylation of ERK1/2 kinases. The Journal of biological
chemistry 284, 22048-22058.
1. Chatr-aryamontri, A., Ceol, A., Peluso, D., Nardozza, A., Panni, S., Sacco, F., Tinti, M., Smolyar, A.,
Castagnoli, L., Vidal, M., Cusick, M.E., and Cesareni, G. (2009). VirusMINT: a viral protein interaction
database. Nucleic acids research 37, D669-673.
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