abstract prima parte 2016_11_16 - BtBsDay
Transcript
abstract prima parte 2016_11_16 - BtBsDay
BtBsDay 2016 1-52 GLYCO-NANOTECNOLOGY LAB 4014: Glyconanoparticles and Glyco-Molecular tools for targeting and as modulators-inhibitors-drugs Research Area: 1 - Nanobiotechnologies for preclinical studies Research Team: PI: Prof. Barbara La Ferla (1) Alice Paiotta, PhD student (1), Rana Edwards, PhD student (1), Alessandro Palmioli, Post Doc (1) Marta Rosati, master student (1) (1) Dep. Biotechnology and Bioscience, Piazza della Scienza 2, I-20126, Milan, Italy [email protected] Abstract Multivalent carbohydrate-carbohydrate and carbohydrate-protein interactions are at the base of a plethora of biological (physiological/pathological) process, including virus/bacterial adhesion, cancer cell proliferation and metastases, immune response, etc. In this contest we design and synthesize multivalent glycosylated scaffolds, including sugar decorated PLGA-NP and glycodendrons, to study cellular interactions and respons. Preliminary studies using mannitol decorated NP showed their ability to increase permeability of the blood brain barrier (BBB), opening the way for a controlled delivery of drugs to the brain. Another topic of our lab deals with glycol-based NPs, the so called cellulose nanocrystals (CNCs). Despite there are examples of drug loaded CNCs in the literature, there is no study of in-vivo pharmacokinetic study of such NPs. We are addressing this topic and preliminary data show an important tropism towards bone tissue, promising fact for the treatment of bone related pathologies. CNCs are also being studied as saccharidic polymers able to inhibit bacterial adhesion. Glyco-Molecular tools: in this context we are developing selective inhibitors/modulators of the hexosamine biosynthetic pathway, as potential antitumoral drugs and as chemical tools for the treatment of related pathologies. We are also synthesizing a library of peptidomimetics based on a glycidic scaffold, for the specific targeting of the gastrin releasing peptide receptor (GRPR) overexpressed in a plethora of tumor cells, and with potential antitumor activity. Biosensors: The selective detection of biomolecules is a key feature for the development of novel and high-throughput diagnostic tools. In collaboration with the CNR institute we are developing a carbohydrate functionalized electrode able to selectively detect the presence of lectines in solution, with a very high sensitivity. Collaborations: UNIMIB - Prof. Marco Orlandi / Dr. Luca Zoia , Dr. Giulio Sancini, Dr.ssa Cristina Airoldi, Dr. Ferdinando Chiaradonna, Dr. Patrizia Di Gennaro CNR - Dr.ssa Barbara Vercelli Mario Negri Institute - Dr. Paolo Bigini Acknowledgments: This project has received funding from the European Union’s HORIZON 2020 Programme for research, technological development and demonstration under grant agreement no. 642028 H2020-MSCA-ITN-2014