Strongest Pill Ever - Ipertrofia Prostatica Cura Con Cialis

Enhancement of high mass sensitivity on the Bruker UltraFlextreme
with FlashDetector technology using the CovalX HM2.
Introduction
Traditionally, MALDI-TOF instrument used microchannel plates (MCP) for ion detection. MCP’s must first
generate secondary electrons which are then amplified and their sensitivity therefore decreases with
increasing ion mass. Often MCP’s will also saturate when measuring complex samples. In order to reliably
measure ions at these higher masses, a more sensitive detector is required. The Bruker UltraFlextreme has
recently been developed featuring a FlashDetector. This type of detector does not rely upon MCP detection
and therefore can be used to measure higher mass ions. Additionally a high mass detection system for high
mass detection is now commercially available. In this poster, these two new ion detectors were evaluated
using identical conditions and the effects of sensitivity and saturation were evaluated.
Ryan Wenzel1; Young-Ho Chung2; Alexis Nazabal3
1CovalX
2Korea
Insulin
150μM
Methods
A Bruker UltraFlextreme MALDI–TOF/TOF instrument (Bremen, Germany) was
retrofitted with a novel high mass detection system (HM2; CovalX AG, Zurich,
Switzerland) installed directly after the reflectron in linear mode. The HM2 is a
small vacuum chamber which can mechanically move the high mass detector
under vacuum in-line of the ion flight path directly in front of the
FlashDetector. The CovalX high mass detector is designed using a high voltage
collision dynode surface positioned in front of a secondary electron multiplier
(CD-SEM). This setup allows for easy evaluation of CD-SEM to the
FlashDetector within a matter of seconds on the exact same sample inside the
same instrumental conditions.
Comparison Analysis
Bruker UltraFlextreme
retrofitted with a CovalX HM2
High-Mass detector system
Four different proteins covering a broad mass range (5.7kDa to 1.1MDa)
were analyzed at relatively high concentrations. Multimer peaks from
each are shown as examples of MALDI “plume-adducts” and are used to
show the high mass sensitivity.
+
UltraFlextreme
FlashDetector
CovalX HM2
Insulin (5.7kDa)
68kDa (12-mer)
136kDa (24-mer)
BSA (66kDa)
256kDa (4-mer)
462kDa (7-mer)
Immunoglobulin G (150kDa)
450kDa (3-mer)
850kDa (5-mer)
Immunoglobulin M (1.1MDa)
barely 570kDa
easily 1.1MDa
Bovine Serum Albumin
20 μM
UltraFlextreme
Flash Detector
12Insulin
UltraFlextreme
Flash Detector
+
4BSA
CovalX HM2
CovalX HM2
+
24Insulin
7BSA
Immunoglobulin G
9μM
+
3IgG
All analysis are preformed on the exact same sample spots, using the
exact same experimental conditions (voltages, laser, # shots). The ONLY
change is that the CovalX detector position is moved in/out of the flight
path. The spectra shown were acquired within minutes (sometimes
seconds) of one another.
Shown spectra have no smoothing or data processing applied. However,
the FlashDetector spectra were recalibrated to show correct masses
because of the differences in flight times between the two detectors
Instruments Incorporated, Saugus, MA
Basic Science Institute, Daejeon, SOUTH KOREA
3CovalX AG, Zürich, SWITZERLAND
www.covallx.com [email protected]
Results/Spectra
Immunoglobulin M
0.6μM
UltraFlextreme
FlashDetector
CovalX HM2
5IgG
+
+
IgM
+2
UltraFlextreme
Flash Detector
CovalX HM2
IgM
+
Conclusions
While the latest FlashDetector technology utilized in the Bruker UltraFlex system provides noticeable
improvement over the MCP technology utilized in previous Bruker instruments; there is still a significant
difference in detection efficiency when compared to the CovalX High Mass Systems.
Approximately twice as large of ion masses are detected under identical conditions when using the CovalX
high mass systems. There is also a noticeable increase in total ion intensity measured and signal-to-noise
with the CovalX system
These detection differences become more noticeable the higher in mass that ions are measured or the
more complex the sample signal is.
Acknowledgments
The authors acknowledge SunMoon Min from SMAnalytical and the researchers at KBSI for coordinating this work. The
authors also acknowledge Jan Streller and the rest of the Bruker team which made this installation such a smooth process.