Relazione-ScientificaAprile2013

Transcript

Scuola di dottorato di ricerca in Nanotecnologie
Resoconto dell’attività 2012
Sommario
1. Relazione scientifica riassuntiva .................................................................................................. 3 2. Elenco dottorandi, supervisori e titolo delle tesi .......................................................................... 6 3. Matrice delal attività di Ricerca della scuola di nanotecnologie ............................................... 11 4. Schede attività dottorandi........................................................................................................... 14 Dottorandi del 24 e 25 ciclo: • ............................................................................................................ 15 Dottorandi del 26 ciclo:....................................................................................................................... 52 Dottorandi del 27 ciclo ........................................................................................................................ 87 Progetti Dottorandi del 28 ciclo: ......................................................................................................... 88 5. Elenco dei componenti del collegio docenti ............................................................................ 164 6. Produzione scientifica del collegio docenti 2007-2012 ........................................................... 167 Personale di ruolo nelle università italiane e INAF .......................................................................... 167 Personale non di ruolo nelle università o dipendenti di altri enti...................................................... 197 7. Criteri di valuatazione delle scuole di dottorato ...................................................................... 204 8. Giudizi dei referee esterni per dottorandi ammessi all’esame finale aprile 2013 .................... 207 9. Presentazioni dei dottorandi al congesso del gennaio 2013 ..................................................... 209 Attività della scuola: situazione ad aprile 2013.
Questo documento contiene il dettaglio dell’attività didattico scientifica della scuola di dottorato in
nanotecnologie svolto durante l’anno 2011 fino ad aprile del 2012. Il documento si compone delle
seguenti parti:
1. Relazione scientifica riassuntiva (Executive summary)
2. Elenco dei dottorandi, supervisori e titolo tesi in corso.
3. Schede di attività dei dottorandi che hanno svolto attività continuativa nell’anno 2011. Questa
attività è stata presentata nel congresso di gennaio 2012 (vedi slides su CD allegato) ed è
riassunto per ogni dottorando nelle schede riportate di seguito. Le schede si riferiscono ai
dottorandi in corso dei cicli 24, 25 e 26, non esssendoci dottorandi in proroga da cicli
precedenti.
4. Progetti dei dottorandi nuovi entrati nella scuola (27 ciclo). Questa attività non è stata presentata
in gennaio 2012 poiché non ancora definiti gli accoppiamenti dottorando – tema di ricerca.
5. Riassuntivo delle pubblicazioni dei dottorandi.
6. Giudizi della commissione d’esame finale 2012.
7. Elenco dei componenti collegio docenti.
8. Pubblicazioni rappresentative del collegio docenti.
9. Criteri di valutazione delle scuole di dottorato.
10. Valutazione della scuola da parte del nucleo di valutazione riassunta in una tabella e rimandi ad
allegati.
11. Giudizi dei reviewer esterni per i dottorandi ammessi all’esame finale (allegati).
12. Presentazioni dei dottorandi al congresso gennaio 2012 (allegati).
1. Relazione scientifica riassuntiva
La missione della scuola di dottorato è di migliorare le conoscenze ed educare studenti nel campo delle
nanotecnologie al fine di formare scienziati e tecnici per il 21° secolo. La caratteristica della Scuola è
l’interdisciplinarità: sugli argomenti di ricerca attivi lavorano in sinergia fisici, chimici, biologi,
ingegneri, medici, farmacologi, odontoiatri, biotecnologi e laureati in Agraria, mantenendo e
rafforzando la specificità della cultura di provenienza ed acquisendo la capacità di sviluppare la propria
ricerca in un quadro più ampio.
L’obiettivo principale della Scuola è di formare Ricercatori che sappiano progettare, costruire,
utilizzare e sottoporre a prove di funzionalità strumenti e dispositivi nano tecnologici, in grado di
rispondere alle crescenti e diversificate esigenze delle applicazioni. L'allievo “dottorato” di questa
Scuola sarà un professionista della ricerca e dello sviluppo tecnologico che sappia applicare le proprie
conoscenze, con capacità di valutazione critica, allo sviluppo di metodi di progettazione, produzione e
valutazione di nuovi materiali e al miglioramento di quelli esistenti. Questo anche mirato ad una
produzione industriale più efficace, economica e sostenibile dal punto di vista delle risorse e
dell'ambiente. La scuola è stata fondata nel 2006 a partire da un dottorato in nanotecnologie.
Gli obiettivi delle ricerche sono i seguenti:
•Sviluppo di nuove tecniche sperimentali per lo studio, la lavorazione, la manipolazione e la
visualizzazione su scala nanometrica di materiali nanostrutturati.
•Sviluppo di tecniche spettroscopiche di rivelazione di singola molecola su substrati
nanostrutturati.
•Studio delle relazioni tra la microstruttura e le proprietà dei materiali e ingegnerizzazione di
materiali nanostrutturati.
•Sintesi di nanostrutture.
•Applicazioni delle nanotecniche e nanostrutture a ricerche di interesse biomedico ed energetico.
•Modellizzazione molecolare multiscala di materiali e di fenomeni di interesse attraverso tecniche
di simulazione computazionale.
•Salute umana con particolare attenzione allo studio ed al trattamento di tumori e malattie
degenerative.
•Applicazione delle nanotecnologie nei settori medico, farmacologico, biomedico ed agroalimentare.
Questi obiettivi sono perseguiti avvalendosi delle attrezzature d'avanguardia disponibili nei laboratori
dell'Università di Trieste e degli Enti di ricerca pubblici e privati convenzionati con l’Università di
Trieste, come da seguente tabella:
n.
1.
2.
3.
4.
5.
6.
7.
8.
Tipologia del soggetto
Istituto/Ente di Ricerca non
(compreso IRCCS)
(compreso IRCCS)
(compreso IRCCS)
(compreso IRCCS)
(compreso IRCCS)
(compreso IRCCS)
Privato non di ricerca
(compreso IRCCS)
accademici
Pubblico/Privato
PUBBLICO
Denominazione del soggetto
Sincrotrone Trieste S.C.p.A.
accademici
PUBBLICO
Laboratorio Nazionale TASC-IOM CNR
accademici
PUBBLICO
accademici
PUBBLICO
CNR - Institute of Chemistry of OrganoMetallic
Compounds
IRCSS Burlo Garofolo - Trieste
accademici
PUBBLICO
CRO Aviano
accademici
PUBBLICO
accademici
PRIVATO
PUBBLICO
Istituto Nazionale dei tumori di Milano Fondazione
IRCSS
Indistrie Bracco
Fondazione Callerio - Trieste
Gli studenti di dottorato nel periodo in esame hanno svolto stage presso aziende ed enti di ricerca. I più
significativi sono i segnueti:
STAGE IN ITALIA
n.
1.
2.
3.
4.
5.
6.
Pubblico/Privato
PUBBLICO
PUBBLICO
PUBBLICO
PUBBLICO
MISTO
PUBBLICO
CNR-IOM TASC Laboratorio Trieste
Elettra Sincrotrone Trieste
IRCCS Burlo Garofalo
CRO Aviano
Centro Biomedicina Molecolare (CBM)
Università di Udine
STAGE ALL'ESTERO
n.
1.
2.
3.
4.
5.
6.
7.
8.
9.
Max Plank Institute - Halle
CNRS - Marsiglia
University Poznan - Center for nanotechnology
Ohio University, Athens (OH)
Technische Universität di Monaco di Baviera
Soft Matter Physics Division - Leipzig
Università di San Pietroburgo
Technische Universitaet - Institut fuer Chemie
Max-Volmer-Laboratorium für Biophysikalische
Chemie
Università della Svizzera Italiana
Paese
Germania
Francia
Polonia
Stati Uniti d'America
Germania
Germania
Russai
Germania
Svizzera
Di seguito alcuni numeri rappresentativi dell’attività della scuola:
• Numero di studenti (ad aprile 2013): 70
• Numero di diplomati nel 2013: 10
• Membri del collegio docenti: 44
• Enti di ricerca coinvolti nel dottorato:
oDipartimenti dell’Università di Trieste: 5 (in diminuzione per accorpamenti)
oEnti di ricerca esterni: 10
oAltre Università: 4
• Numero di pubblicazioni degli studenti:
oAnno 2007: 67
oAnno 2008: 68
oAnno 2009: 72
oAnno 2010: 105
oAnno 2011: 95
oAnno 2012: 98
• Numero di pubblicazioni di supervisori e tutors (2007-12): oltre 1000
• Risorse finanziarie (per anno): 700.000 euro
• Ultima valutazione complessiva del nucleo dell’Università di Trieste: A+ (Ottimo)
I criteri usati per la valutazione interna sono stati quelli suggeriti dal Ministero, accompagnati da criteri
interni all’Ateneo. Questi criteri sono descritti di seguito in questo documento in modo dettagliato. I
principali indicatori utilizzati dal nucleo che hanno permesso la valutazione in classe A+ sono (vedi
capitolo 10 per maggiori dettagli):
• produzione scientifica del Collegio Docenti;
• congruo numero di docenti dell’Ateneo coinvolti nel collegio;
• rapporto del dottorato con il mondo del lavoro;
• attrattività del dottorato nelle precedenti edizioni;
• livello di soddisfazione dei dottorandi;
• produzione scientifica dei dottorandi ed iniziative esistenti di pubblicizzazione e referaggio
delle tesi di dottorato;
• adeguata formulazione delle tematiche scientifiche e degli obiettivi formativi,
• riduzione della frammentazione dei corsi di dottorato;
• accuratezza delle informazioni fornite per la valutazione;
• presenza di iscritti stranieri;
• sito web del dottorato.
2.Elenco dottorandi, supervisori e titolo delle tesi
La situazione riportata in tabella è quella ad aprile 2013, quindi include i dottorandi del 28 ciclo e non
quelli del 25 ciclo diplomati ad aprile.
Dottorando
Ciclo Supervisore
ABDOLLAHZADEH Iman
AMODIO Alessia
27 SCOLES Giacinto
28 TOFFOLI Giuseppe
ANGELONI Valeria
27 BRESCHI Lorenzo
BORIN Daniele
27 LAZZARINO Marco
28 PASCOLO Lorella
CAMMISULI Francesca
SSD
NomeDip
FIS/03
Department of Physics ‐ DF
Nanotechnology approaches for the detection of circulating tumor cells
BIO/14
Quantitative analysis of tumor suppressor and ancogene proteins in tumor micro‐sample
MED/28 Clinical Department Role of collagen cross‐linkers on the of Medical Science, stability of bonded interface
Surgery and Health ‐ DCSMCS
FIS/03 Department of Micro mechanical oscillators for Physics ‐ DF
biochemical applications
BIO/14 Clinical Department Effetti dei nano materiali sulle of Medical Science, barriere biologiche fetali e post‐natali Surgery and Health ‐ e valutazioni della tossicità epigenica
DCSMCS
FIS/03 Department of Capacitance immunosensors for the Physics ‐ DF
early detection of circulating cancer biomarkers
CAPALDO Pietro
28 CASALIS Loredana
CAPOLLA Sara
27 MACOR Paolo MED/04 Department of Life Science ‐ DLS
CASSESE Damiano
26 LAZZARINO Marco
CECCHINI Paolo
27 TOGNETTO Daniele
COCEANO Giovanna
CORAL Lucia
27 COJOC Dan
CORVAGLIA Stefania
DAGOSTINO Ilenia
28 TOFFOLI Giuseppe
26 CASALIS Loredana
28 GAMINI Amelia
Titolo Tesi
Use of chemotherapic‐loaded nanoparticles in the treatment of cancer
FIS/03 Department of Design and realization of Physics ‐ DF
nanoelectromechanical and plasmonic devices for Raman spectro‐
microscopy
MED/30 Clinical Department Bio‐Materials in oculist micro surgery
of Medical Science, Surgery and Health ‐ DCSMCS
FIS/03
BIO/14 Department of Physics ‐ DF
FIS/03 Department of Physics ‐ DF
CHIM/04 Department of Life Science ‐ DLS
Characterization of the mechanical properties of cancer cells
Detection of tumor cell surface biomarkers in microsamples
Nanoscale platform to study unstructured proteins interactions
Valorizzazione di matrici alimentari della dieta mediterranea con l’arricchimento di nutrienti
Dottorando
Ciclo Supervisore
SSD
DAL COL Valentina
26 PRICL Sabrina ING‐
IND/24
DE BIASI Matteo
28 ANGERAME Daniele
DEL BEN Fabio
28 SCOLES Giacinto
DIOLOSA‘ Marina
27 CADENARO Milena
ELISEI Elena
FORNASARO Stefano
NomeDip
Department of Engineering and Architecture ‐ DIA
MED/28 Clinical Department of Medical Science, Surgery and Health ‐ DCSMCS
FIS/01 Department of Physics ‐ DF
27 CESARO CHIM/04 Department of Life Attilio
Science ‐ DLS
27 PASSAMONTI BIO/10 Department of Life Sabina
Science ‐ DLS
Titolo Tesi
In silicio prediction of drug resistance: from cancer targeted therapy to cancer targeted prevention
Materiali nano‐strutturati per l’odontoiatria
Development of nanodevice to explore cell network in cancer
Development of a new nano‐
engineered biopolymer‐based dental adhesive system
Nanostructured biomolecular glasses
Targeting natural antioxidant compounds to the brain: a metabolomic assessment.
MED/28 Clinical Department Nanostructural analysis of the of Medical Science, adhesive interface in dentistry
Surgery and Health ‐ DCSMCS
FIS/03 Department of Development of quantum well Physics ‐ DF
structures for multi band photon detection
FIS/03 Department of Syntesis of ordered semiconductor Physics ‐ DF
nanostructures by directed self‐
assembly for photonic applications
MED/28 Clinical Department The influence of resin‐based dental of Medical Science, materials upon oral biofilms Surgery and Health ‐ development.
DCSMCS
FRASSETTO Andrea
26 CADENARO Milena
GANBOLD Tamiraa
27 BIASIOL Giorgio
HUSSAIN Sajid
26 BIASIOL Giorgio
IONESCU Andrei Cristian
27 CADENARO Milena
LOVAT Giacomo
27 MORGANTE Alberto
MARSON Domenico
27 PRICL Sabrina ING‐
IND/24
MINIUSSI Elisa
26 BARALDI Alessandro
FIS/03
MITRI Elisa
26 TORMEN Massimo
FIS/03
FIS/03
Study of charge transfer processes at interfaces for photovoltaic applications
Computer‐assisted design of nanovectors for gene therapy
Interaction of metal nanoclusters with graphene and low dimensional systems
Fabrication of microfluidic devices for studying living cells, responding to external stimuli, by vibration spectroscopies
Dottorando
MOHAMMED Khalid Amna
Ciclo Supervisore
SSD
28 ONESTI Silvia FIS/03
NomeDip
Titolo Tesi
Atomic force microscopy investigation of the structural properties of DNA replication origins in tumor cells
High sensitivity detection of DNA/miRNA targets in AFM nanografted arrays
NKOUA NGAVOUKA Maryse Dadina
27 CASALIS Loredana
FIS/03
OMICIUOLO Luca
28 BARALDI Alessandro
FIS/03
OTTAVIANI Giulia
27 ZACCHIGNA Serena
Use of nanotechnology for the evaluation of the effect of laser therapy on neo‐angiogenesis tumor in vivo and in vitro
PALMA Giuseppina
26 FRALEONI Alessandro
FIS/03
Nanostructured dye‐sensitized solar cells
PANIGHEL Mirco
27 MORGANTE Alberto
FIS/03
Organic Molecules and Carbon Nanotubes Assembled on Metal Surfaces:Properties and Applications in Molecular Electronics and Photovoltaics
PATERA Laerte Luigi
PELLIZZONI Elena
28 AFRICH Cristina
28 TOFFOLI Giuseppe
27 LAZZARINO Marco
FIS/03
Studio in situ e inoperando di processi catalitici su superfici metalliche
Interactomics in tumor cells studied with a nanotechnology approach
Plasmonic ruler and the applications to the DNA origami
27 CESARO Attilio
28 PAOLETTI Sergio
BIO/10 Department of Life Science ‐ DLS
RADIVO Andrea
26 TORMEN Massimo
FIS/03
ROMEO Michele
26 FRONZONI Giovanna
PIANTANIDA Luca
PITTIA Paola
PORRELLI Davide
ROMERO Ocana Ismael
SACCO Pasquale
BIO/14
FIS/03
CHIM/02 Department of Chemical and Pharmaceutical Science ‐ DSCF
28 FORNASIERO CHIM/03 Department of Paolo
Chemical and Pharmaceutical Science ‐ DSCF
28 PAOLETTI BIO/10 Department of Life Study of the growth processes and of the physical and chemical properties of graphene‐based low dimensional systems
Nanotecnology for food science
Nanocostruttori polimerici a matrice polisaccaridica quali biomateriali bioattivi per impieghi in chirurgia
Experimental study of the physics of nanostructured organic photovoltaic devices.
Development and applications of methodologies TDDFT for the simulation of core spectrum for condensed matter.
Combustibili solari
Nuovi biomateriali per terapie Dottorando
Ciclo Supervisore
Sergio
SANTESE Francesca
26 FERMEGLIA Maurizio
SCOGNAMIGLIO Francesca
SSD
NomeDip
Science ‐ DLS
Titolo Tesi
innovative nel trattamento delle ferite difficili
ING‐
IND/24
Multiscale molecular modeling for nanostructured multifunctional materials and coatings
28 PAOLETTI Sergio
BIO/10
Department of Life Science ‐ DLS
Sviluppo di un sistema adesivo per applicazioni odontoiatriche in grado di formare legami chimici sia con il collagene della dentina che con la resina riparativa
SCOTTI Nicola
27 BRESCHI Lorenzo
MED/28 Clinical Department Laboratory evaluation of several of Medical Science, nanofilled dental resin composites Surgery and Health ‐ mechanical and chemical properties
DCSMCS
STOPAR Alex
27 TOFFOLI Giuseppe
FIS/03
SVETINA Cristian
28 ZANGRANDO FIS/03
Marco
TARUSHA Lorena
27 PAOLETTI Sergio
BIO/10
Department of Life Science ‐ DLS
TAVAGNACCO Letizia
26 CESARO Attilio
VAIDYA Shital
26 GOTTER Roberto
FIS/03
VEGA Marienette
27 SERGO Valter ING‐
IND/22
VELARI Simone
28 DE VITA Alessandro
ING‐
IND/22
VENTURELLI Leonardo
27 SCOLES Giacinto
FIS/03
VIRGILIO Francesca
26 TORMEN Massimo
FIS/03
WANG Lianqin
27 FORNASIERO CHIM/03 Department of Department of Life Science ‐ DLS
Innovative Tools for Pharmacogenetics and Pharmacogenomics: Protein‐Nucleic Acid Interactions Within Self‐
Assembled Nanosystems
Photon beam studies and characterization for EUV/Soft X‐ray coherent imaging of nano‐structures using coherent FEL radiation
Novel nanostructured biomaterials for biomedical applications
How do water molecules probe and control bionanostructures and food functionalities
Low‐dimensional magnetic materials: the electronic structure and correlation effects
Raman and fluorescence spectroscopy of biomedical nanomaterials
Modelling the atomic scale properties of simple biomolecules at surfaces: from the role of solvent to small structured peptides.
Microfluidic Devices for Circulating Tumor Cells Counting
Development of electrochemical sensors by nanofabrication techniques for biological and medical diagnostics
Design, synthesis and characterization Dottorando
Ciclo Supervisore
Paolo
SSD
YOUSAFZAI Muhammad Sulaiman
28 COJOC Dan
FIS/03
ZANNIER Valentina
27 RUBINI Silvia FIS/03
NomeDip
Chemical and Pharmaceutical Science ‐ DSCF
Titolo Tesi
of nanostructured materials for electrocatalysis
Synthesis and characterization of semiconductor nanowires
Study of mechanotransduction signaling in tumoral and metastatic cells
3.Matrice della attività di Ricerca della scuola di nanotecnologie
N. Ph.D. student
N. Ph.D. student
1 ABDOLLAHZADEH Iman
29 PERONIO Angelo 2 ANGELONI Valeria
30 SAMMITO Davide 3 BORIN Daniele 31 TARUSHA Lorena
4 CAPOLLA Sara
32 ZANNIER Valentina
5 CECCHINI Paolo
33 VENTURELLI Leonardo
6 GANBOLD Tamiraa
34 WANG Lianqin 7 DIOLOSA‘ Marina
35 VEGA Marienette
8 ELISEI Elena 36 PITTIA Paola
9 FORNASARO Stefano 37 STOKELJ Tina
10 COCEANO Giovanna
38 PALMA Giuseppina
11 IONESCU Andrei Cristian
39 CORVAGLIA Stefania
12 LOVAT Giacomo 40 HUSSAIN Sajid
13 MARSON Domenico
41 MINIUSSI Elisa
14 NKOUA NGAVOUKA Maryse Dadina
42 MITRI Elisa
15 OTTAVIANI Giulia
43 CASSESE Damiano 16 PANIGHEL Mirco
44 VAIDYA Shital
17 PIANTANIDA Luca 45 ROMEO Michele
18 SALGADO 46 GANAU Mario Simâo Pedro
47 ZANUSSO Chiara 19 SCOTTI Nicola
48 MARSICH Lucia 20 STOPAR Alex
49 LUCAFO’ Marianna 21 VIRGILIO Francesca
50 CHEN Yan Xin 22 SANTESE Francesca
51 FRASSETTO Andrea 23 TAVAGNACCO Letizia 24 RADIVO Andrea
25 DAL COL Valentina
26 AMBROSINI Stefano 27 BIDOGGIA Silvia 28 GIORGIS Valentina BERNARDES PEREIRA Applications of Nanotechnology
Nano
character
ization
Nano fabrication
Methods and Techniques
Micro, Nano Advanced
Pharmaceutics Medicine
& Agro Food (E)
and
Opto Materials
& drug
Life Sciences
electronics (A) biomaterials
development
(D)
(B)
and
drug
delivery (C)
1.1.Nanofabrication bottom 16,26,32,38,40
up
,41,43
1.2.Nanofabrication top
6,28,30,40,43
down
1.3.Nanoparticles
fabrication and
characterization
1.4.Self assembly
3, 16,40,41
1.5.Development of
MEMS and NEMS
1.6.Development of new
nanofabrication
techniques /
instruments
1.7.Templated
growth/deposition
2.1.Single molecules
detection
2.2.Living cells
characterization
11,
20, 14
26,31,38,41
20,
17,31,42,46
Energy
&
Environment
(F)
16,24,26,32,38
,50
24,30,50
7,
17, 27,49
27,31,35,41,51
4,
9,27,31,35,48
17, 20, 27,41
20, 27,39,46
14, 27
3
36
34
5, 43
36
38,50
2,3, 17
23
29
3, 43
6,38
38
16,28,40
41
3
2
14
11,31
42
12
1,4,9,10,11,15,
31,33,42
Nano
modelingtheory and
2.3.Characterization
techniques/ instruments
3,38
11,19,31,35,38
47
1,3,9,11,17,19,
31,33,35,39,43
2.4.Imaging & diagnostics
3,40
8,2,11
8
2,3,4,5,8,10,11
,15,17,20,21,4
2,46,47
2.5.Toxicity
11,31
9,42,47,49
11, 15,31
2.6.Investigations of
dynamical processes
2.7.Novel characterization
techniques
2.8.Nano characterization
of materials
2.9.Low-dimensional
systems
2.10.Electronic and
geometric structure of
solid surfaces
3.1.Multiscale molecular
modeling
3.2.Ab initio molecular
modeling
3.3.Development of new
theory / methods
41
42,47
9, 17
34
20,39,43
38
38,44
27,38,44
12,32,40,41
8,27,35,41
16,32,40,41,44
41,44
16,41
41
28,30
18,22
12,45
45
45
45
8,27
8
9
29
34,38
37
20
12,29,34
29,34
29,34
13, 25
25
23,37
18
12,29
47
Number refers to the Ph.D. students. In Red the main them (one per student).
13
4.Schede attività dottorandi
Questo allegato contiene le schede dei dottorandi della scuola di nanotecnologia così come
sono pervenute al 31 gennaio 2013. Sono quindi incluse le schede dei dottorandi dei cicli 25,
26 e 27.
• Dottorandi del 24 cilco: ZANUSSO Chiara (estensione di un anno)
• Dottorandi del 25 ciclo: AMBROSINI Stefano, BIDOGGIA Silvia, CHEN Yan Xin,
GANAU Mario, GIORGIS Valentina, LUCAFO' Marianna, MARSICH Lucia,
PERONIO Angelo, SAMMITO Davide
• Dottorandi del 26 ciclo: CASSESE Damiano, CORVAGLIA Stefania, DAL COL
Valentina. FRASSETTO Andrea, MINIUSSI Elisa, MITRI Elisa, PALMA
Giuseppina, RADIVO Andrea, ROMEO Michele, SAJID Hussain, SANTESE
Francesca, TAVAGNACCO Letizia, VAIDYA Shital, VIRGILIO Francesca.
• Dottorandi del 27 ciclo: ABDOLLAHZADEH Iman, ANGELONI Valeria, BORIN
Daniele, CAPOLLA Sara, CECCHINI Paolo, COCEANO Giovanna, DIOLOSA‘
Marina, ELISEI Elena, FORNASARO Stefano, GANBOLD Tamiraa, IONESCU
Andrei Cristian, LOVAT Giacomo, MARSON Domenico, NKOUA Ngavouka
Maryse Dadina, OTTAVIANI Giulia, PANIGHEL Mirco, PIANTANIDA Luca,
PITTIA Paola, SALGADO Bernardes Pereira Simâo Pedro, SCOTTI Nicola,
STOKELJ Tina, STOPAR Alex, TARUSHA Lorena, VEGA Marienette,
VENTURELLI Leonardo, WANG Lianqui, ZANNIER Valentina.
14
Dottorandi del 24 e 25 ciclo: •
STEFANO AMBROSINI
Title of the thesis: Growth and characterization of nanostructures
Supervisor: Silvia Rubini
RESEARCH ACTIVITY
The aim of my Ph.D. was the synthesis and characterization of nanostructures. One of the
most sought after topics of the nanoscience in the recent years are semiconductor nanowires,
of which the host research group had experience, especially regarding GaAs. Bearing this aim
in mind and given the rising interest into non-Au-related protocols to synthesize
semiconductor nanowires (accounting the well known incompatibility of Au with Si-based
technology), the first period of my Ph.D. was spent on the study and engineering of a suitable
substrate to obtain the self catalyzed (Au free) nanowire growth. We found that by depositing
Si onto epiready GaAs wafers, or onto thermally deoxidized wafers afterwards exposed to air,
a thin silicon oxide layer was formed. Exposing such substrates (Si-treated substrates) at Ga
and As fluxes resulted in epitaxially oriented, full zincblende, Ga catalyzed GaAs nanowires.
We eventually found the dependences of the nanowire yield on the growth temperature,
material fluxes, Si layer thickness and substrate crystal orientation.
GaAs material suffers from crystal polytypism given by the hexagonal wurtzite structure and
the cubic zincblende, the cohesive energy difference among them being few tens of meV.
Such polytypism was a crucial problem affecting both Au- and self catalyzed GaAs
nanowires. Therefore a big part of my Ph.D. was dedicated to the study of the dependence of
such polytypism on growth conditions and post growth processes (more on this later). The
dependence on the growth conditions were investigated and found by means of MBE in
Trieste. In particular we found that when the material ratio leans towards As-rich conditions,
the Ga catalyst nanoparticle shrinks and consequently the most favoured crystal structure
becomes hexagonal, over the default cubic stacking. Eventually, this study led to hints of
GaAs nanowire growth in absence of any catalyst at all. Deeper studies though resulted into
bizarre and not fully understandable results, the main problem being a too wide dispersion of
the experimental data, that made any trend not reilable.
In collaboration with dott. Giacomo Priante at TASC IOM CNR (Trieste, Italy), self catalyzed
GaAs nanowire were later on found to resume the catalyzed growth after the Ga catalyst
consumption in As flux, in the correct (Ga rich) conditions. Electron microscopy studies
(performed in Saint Petersburg State university) unveiled details of the Ga catalyst renucleation, gave the trends for the axial re-growth and the lateral over-growth and helped the
formulation of a theory model to explain such a behaviour, not previosly remarked in
15
literature, the last point thanks to the collaboration with prof. Vladimir Dubrovskii from Saint
Petersburg Accademic University. In particular, such growth model states that GaAs
nanowires under our conditions grow in net Ga-rich conditions with negligible adatom
diffusion on the nanowire sidewalls.
Post growth annealing step were thought to be a solution to the GaAs polytypism. By giving
thermal energy to the structures, the metastable hexagonal structure, (common in Au
catalyzed nanowires
), was thought to change to the default bulk cubic structure, whose energy is lower also for
nanowires with diameter on the scale of interest – 100 nm).
This thesis was checked by means of TEM at DTU Nanotech and DTU CEN (Copenhagen,
Denmark). Some of the experiments confirmed the theses but a too low statistics led to the
conclusion that TEM was not the best technique to assess such physics. Therefore, in
collaboration with dott. Damiano Cassese at TASC IOM CNR (Trieste, Italy) we approached
the same task by means of Raman spectroscopy. In particular we compared the intensity of
the optical phonons of the hexagonal and cubic structure, that exhibit a different and clearly
distinguishable energy and found that after 100 hours at 600 °C in As atmosphere bundles of
Au catalyzed hexagonal nanowires were nearly completely transformed to cubic structure.
This demonstrated the hypotheses, although only on nanowire bundles measurements. In
single nanowire analyses, in fact, no such a clear result was found, and the same data
dispersion found by means of TEM was encountered.
The last part of my Ph.D. was spent in Russia, at Saint Petersburg State and Academic
Universities, studying nanowire applications and devices, MBE growth and TEM microscopy.
The main results so far have been the synthesis of Au catalyzed GaAs nanowires on Si (111)
capable of intense THz emission when stimulated by a femtosecond pulsed green laser, and
the synthesis of self catalyzed GaAs/AlGaAs core-shell nanowires, responsible of intense,
linearly polarized photoluminescence. Electron microscopy characterization activity covered
the study of several types of nanostructures (synthesized in Trieste and or in Saint
Petersburg).
A brief visit to Durham Science Site at Durham, UK, allowed me to be trained onto FIB
usage, in order to pattern by top down means silicon substrates, with the aim of spatially
localize the later grown nanowires. No satisfactory result regarding such part of my project
are yet available.
The collateral, concomitant synchrotron activity along the 3 years has been dedicated to
contactless conductivity measurements by means of spatially resolved X-ray photoemission
spectroscopy on GaAs nanowires at ELETTRA synchrotron facility, Trieste, Italy. By
continuing the research line of the previous Ph.D. student, Fauzia Jabeen, we found that Ga
catalyzed nanowires showed a much lower electrical conductivity as opposed to the Au grown
16
wires. We explained this fact invoking the higher purity of the self catalyzed wires. We also
compared the conductivity of catalytically grown wires with the non catalyzed: Si doping of
these wires resulted in p doped structures in the first case (catalyzed wires) and n type in the
second (non catalyzed). We explained this fact observing that the non catalyzed growth
occours in As rich conditions, thus forcing the amphoteric Si to occupy the Ga sites in GaAs,
resulting then in n doping. Recently, a collaboration with the canadian group of prof. LaPierre
started, devoted to model the surface depletion layer of our nanowires, and obtain quantitative
information on the doping of the nanostructures form the photoemission data.
Publications on scientific journals (printed or in press)
- S. Ambrosini, M. Fanetti, V. Grillo, A. Franciosi and S. Rubini, Journal of Applied Physics
109, 094306 (2011)
- S. Ambrosini, M. Fanetti, V. Grillo, A. Franciosi and S. Rubini, AIP ADVANCES 1,
042142 (2011), selezionato per la rivista Virtual Journal of Nanoscale Science & Technology.
- V.V. Danilov, A.S. Panfutova, A.I. Khrebtov, S.Ambrosini, and D.A. Videnichev, Optics
Letters, Vol. 37, Issue 19, pp. 3948-3950 (2012)
Publications/abstracts in conferences/congresses (national or international)
- Self catalyzed GaAs nanowires on Si-treated epiready (100) GaAs wafers by MBE,
S. Ambrosini, M. Fanetti, V. Grillo, A. Franciosi and S. Rubini, III-V Nanowire workshop,
Bad Honnef (Germany), 02/2011
VAPOR-LIQUID-SOLID
AND
VAPOR-SOLID
GROWTH
PROCESSES:
STRUCTURAL IMPLICATIONS OF CATALIST CONSUMPTION DURING SELFCATALYSED GROWTH OF GaAs NANOWIRES, Stefano Ambrosini, Mattia Fanetti,
Vincenzo Grillo, Alfonso Franciosi and Silvia Rubini, 40esimo congresso della associazione
italiana cristallografia (AIC), Siena, settembre 2011.
- “vapor solid nanowire growth”, NGW12 Saint Petersburg Russia
- “study of the final stages of GaAs nanowire growth by MBE”, SPFKC November 2012
Yekaterinburg Russia
- “Vapor solid and vapor liquid solid growth of GaAs nanowires by MBE”, STRANN
October 2012, Saint Petersburg Russia
Participation to conferences (as speaker)
- Nano2011, Yekaterinburg, 06/11
- NWGW (nanowire growth workshop) June 2012, Saint Petersburg, Russia
- STRANN (New research lines in nanotechnology and nanobiotechnology) September 2012,
Saint Petersburg, Russia
17
- SPFKC (winter school about problems of solid state physics) November 2012 Yekaterinburg
Russia
EDUCATIONAL ACTIVITY
Periods abroad (date and place)
24/01/11 – 24/05/11: DTU Copenhagen, DK
19/06/11 – 01/07/11: Yekaterinburg, Russia
10/12/11 – 20/12/11: Saint Petersburg, Russia
29/04/2012 – 28/12/2012: Saint Petersburg Accademic and State University, Saint Petersburg,
Russia
10/09/2012 – 25/09/2012: Durham Science Site, Durham, UK
Classes followed (date, course, professor, type of course)
02/02/11 – 05/05/11, Transmission Electron Microscopy, Marco Beleggia, Ph.D student class,
DTU CEN, Copenhagen (DK)
Conferences, seminars, advanced courses and other didactic activities
Nanotechnology graduate Summer School, Udine, Italia, july 2012.
Winter school on problems of solid state physics, SPFKC, Yekaterinburg, Russia, November
2012
Support educational activity and teaching
- Atomic Force Microscopy, to nanotechnology master degree students, DTU Nanotech,
Copenhagen (DK) with Zachary Davis, ordinary professor at DTU Nanotech, Copenhagen
(DK).
- 10 hours in russian about semiconductor nanowire growth through molecular beam epitaxy
at Saint Petersburg Academic University, october 2012.
- 4 hours in russian about transmission electron microscopy and epitaxial growth of
semiconductor nanowires at Ural Federal State University, Yekaterinburg, November 2012
18
SILVIA BIDOGGIA
Title of the thesis: Mixed-monolayers protected gold nanoparticles for applications in
medicine.
Supervisor: Prof. L. Pasquato
RESEARCH ACTIVITY
The object of my research project deals with the design and synthesis of organic monolayer
protected gold nanoparticles (AuNPs) with an innovative degree of complexity and
functionality and the evaluation of their application in the biomedical field for the transport
and release of biomolecules and drugs. During the last two years many efforts has been spent
for the design, synthesis and characterization of water soluble NPs coated by hydrogenated
charged ligands (H-ligands) and perfluorinated ligands (F-ligands) with the desired
composition of the monolayer. Over this year, preliminary confocal fluorescence microscopy
studies of uptake in living cells has been performed on these nanoparticles. In particular we
are interested to investigate the ability of NPs to pass cell membrane. The behavior of these
NPs in cell is compared with that of NPs coated by H- and F-ligands ending with PEG
moieties (synthesized during the last two years) that we expect don’t pass cell membrane. All
samples of nanoparticles were labeled with FITC as dye. Absorbance and florescence
experiments have been carried out to calculate the number of fluorescent chains per
nanoparticle. In that contest quenching effect of the gold core and of the fluorinated regions
were analyzed. Preliminary experiments on cells have been performed by Dr. F. Sousa from
Besta in Milan and have shown that both charged nanoparticles and pegylated ones are taken
up by endocytosis. This unexpected behavior seems to be due to -stacking interaction between
FITC moieties of different nanoparticles that is responsible of aggregates formation and the
consequent cellular uptake by the well-known EPR effect. To avoid aggregates formation, the
nature of the fluorescent and the loading have been changed. NPs functionalized with Bodipy,
instead of FITC, have been synthesized and cellular experiments are now in progress.
The organization of H-and F-ligands on the surface of nanoparticles may influence the ability
to pass cellular barrier, beside many other properties. During the first two years we have
investigated the morphology of mixed H- and F- ligands with multiscale molecular
simulations that has given us information about the size and the shape of hydrogenated and
perfluorurated domains present on NPs surface. For this reason in order to investigate the
morphology of monolayers formed by H- and F-ligands by STM, mixed monolayer gold NPs
coated by different ratios of commercially available dodecanthiol and tridecafluoro-1octanethiol have been synthesized and characterized. STM experiments are still in progress.
Publications on scientific journals (printed or in press):
•
C. Gentilini, A. Pace, S. Bidoggia, P. Posocco, P. Franchi, M. Lucarini, S. Pricl and L.
Pasquato “Self-organization of mixtures of fluorocarbon- and hydrocarbon amphiplic
thiolates on the surface of gold nanoparticles: a combined experimental and multiscale
molecular modeling study”, ACS Nano 2012, 6 (8), 7243–7253.
19
Publications/abstracts in conferences/congresses (national or international):
•
•
•
•
•
S. Bidoggia; L. Pasquato; “European Winter School on Physical Organic Chemistry”;
Poster Presentation: “Perfluorinated monolayer-protected Au nanoparticles for
membrane permeation”; Bressanone, Italy, January 30 – February 4, 2011.
S. Bidoggia, P. Posocco, M. Fermeglia, S. Pricl, and L. Pasquato; “Summer School on
Nanotechnology”; Poster presentation: “Control of the morphology of mixed
monolayers protecting gold nanoparticles using perfluoro ligands”; Trieste, September
20-23, 2011.
S. Bidoggia, M. Boccalon, D. Porrelli, L. Pasquato; “Summer School on
Nanotechnology”; Poster presentation: “Perfluorinated monolayer-protected Au
nanoparticles for biological applications”; Udine, July 2-5, 2012.
S. Bidoggia, L. Pasquato; “Spanish Italian Symposium on Organic Chemistry”; poster
presentation: “Self-organization of Mixtures of Fluorocarbon- and Hydrocarbon
Thiolates on the Surface of Gold Nanoparticles”; Tenerife, Spain, February 10-14,
2012.
L. Pasquato, S. Bidoggia, M. Boccalon, D. Porrelli; “Nanomedicine: from molecules to
diagnosis and therapy”; Oral communication: “Toward a new generation of
nanoparticles for therapy and diagnosis”; CNR Rome, October 1-3, 2012.
Participation to conferences (as speaker):
•
•
•
L. Pasquato, S. Bidoggia; Summer workshop on “Supramolecular functional materials”;
oral communication: "Morphology of mixed-monolayers protecting gold nanoparticles",
Bibione 20 - 22 May 2010.
“PhD school of Nanotechnology, Annual Meeting 2011”; University of di Trieste,
January 17-19, 2011.
January 16-18, 2012.
Classes followed (date, course, professor, type of course):
•
Prof. A. Franciosi; “Proprietà fisiche dei materiali” (48 h); Corso di laurea magistrale in
Chimica; February-June 2010.
•
Prof. L. Casalis, prof. A. Morgante, prof. L. Pasquato; “Molecular self-assembling and
nanostructures” (16 h);PhD School of Nanotechnology; June-July 2010.
•
Prof. L. Pasquato; “Materiali organici” (32 h); Corso di laurea magistrale in Chimica;
October-December 2010.
•
•
“PhD school of Nanotechnology, Annual Meeting 2010”; University of Trieste, January
18-20, 2010.
Prof. Hanna Mamzer, Sociology Department of Adam Mickiewicz University in Poznan
(Poland); Seminar “How to present scientific results in public”; University of Trieste,
January 22, 2010.
20
•
Summer workshop on “Supramolecular functional materials”; Bibione, May 20-22,
2010.
•
Veneto Nanotech; Seminar “NanoChallenge and PolymerChallenge”; University of
Trieste, June 15, 2010.
•
Prof. Miguel Nobrega of the University of Minho in Portugal; seminar “Development
of On-Line Monitoring devices for the extrusion process”; University of Trieste, June
15, 2010.
•
Prof. Maurizio Fermeglia; seminar “Use of VPN and U-GOV”; University of Trieste,
June 15, 2010.
•
Scientific meeting “I giovani e la chimica in Friuli Venezia Giulia”; Dipartimento di
Scienze e Tecnologie Chimiche, Udine, September 24, 2010.
•
Presentations of the last year students of the PhD school in Nanotechnology; University
of Trieste, November 23, 2010.
•
Dr. Sangeeta Kale, Associate Professor Defence Institute of Advanced Technology
(India); seminar “Metal oxide nanomaterials in sensors and biomedicine”; University of
Trieste, November 23, 2010.
•
January 17-19, 2011.
“European Winter School on Physical Organic Chemistry”; Bressanone, January 30 –
February 4, 2011.
Prof. Maurizio Prato from the University of Trieste; seminar: ”Biomaterials and
Nanomedicine”; Savoia Excelsior Palace, Trieste, March 30, 2011.
Prof. János Kristóf and Prof. Erzsébet Horváth of the University of Pannonia, Institute
of Environmental Engineering in Hungary; seminar: “Synthesis and structure
elucidation of kaolinite organo-complexes”; University of Trieste, May 3, 2011.
Prof. Maurizio Fermeglia; seminar “Use of VPN and U-GOV”; University of Trieste,
May 3, 2011.
Dr. Hicham Hamoudi of the Université-Paris Sud; seminar: “ Self assembled
monolayers on gold the challenge”; University of Trieste, July 1, 2011.
Dr. Matteo Castronovo; seminar “The effect of confinement on enzymes diffusion and
reactions inside DNA nanostructures”; University of Trieste, July 29, 2011.
“Summer School on Nanotechnology”; Trieste, September 20-23, 2011.
Dr. Cristina Gentilini from the Imperial College of London; seminar: “Biomimetic
scaffold for tissue engineering”; University of Trieste, September 22, 2011.
Presentations of the last year students of the PhD school in Nanotechnology; University
of Trieste, November 29, 2011.
•
•
•
•
•
•
•
•
•
•
•
January 16-18, 2012.
Prof. Norberto Masciocchi and Prof Antonietta Guagliardi of Università dell’Insubria,
“Spring course on Diffraction Methods for Nanostructured Materials“, University of
Trieste, March 15, 2012
21
•
“Summer School on Nanotechnology”; Udine, July 2-5, 2012.
•
•
•
•
•
Support educational activity as tutor of the course Organic Chemistry I with lab, Corso
di Laurea di primo livello in Chimica, first year, University of Trieste, 40 hours, April –
June 2010.
June 2011.
June 2012.
Tutor of a dissertation degree of Corso di Laurea di primo livello in Chimica, Title:
Studi per la sintesi di nanoparticelle di oro perfluorurate solubili in mezzi acquosi.
February – May 2011.
Tutor of a dissertation degree of Corso di Laurea di primo livello in Chimica, Title:
Nanoparticelle di oro protette da un monostrato misto di leganti idrogenati/perfluorurati:
sintesi e caratterizzazione. March – July 2012.
22
YAN-XIN CHEN
Title of the thesis: Nanostructured Titanium Dioxide Based Materials for Photocatalysis
and Photoelectrocatalysis
Supervisor: Paolo Fornasiero
Tutors:Claudio Bianchini, Alessandro Lavacchi, Francesco Vizza
RESEARCH ACTIVITY
Titania Nanotubes arrays (TNTAs) are excellent candidates as support materials for
engineering metal catalyzed materials, and has been widely used in the Fuel Cells and also in
the solar production of hydrogen from aqueous solutions of renewable alcohols, due to its
high active surface area; good stability in both acidic and alkaline solutions and cooperative
effects between the TiO2 substrate and the support metal-based catalysts (such as Palladium).
During the whole 3 years PhD study, 4 parts of works have been done.
(1) TiO2 NTs over large flat samples (Ti disk), which with controllable tube length and
width, had been synthesized through the anodization method.
(2) A novel method, ElectroChemical Milling and Faceting (ECMF), for the modification
of Pd Nanoparticles. By which the catalytic activity of supported Pd NPs was huge enhanced
by an order of magnitude for the ethanol electrooxidation.
(3) In order to overcome those major drawbacks for carbon black based electrodes such as
mechanical stability and mass transport limitation, we propose the use of a titanium web with
an ordered anatase nanotube array on top (TNTA-web) as a support for nanostructured Pd
electrocatalyst for the Direct Ethanol Fuel Cells (DEFCs). And compared to the carbon black
based electrodes, TNTA-web based electrodes exhibit much better stability and power density
during the high current density discharge process.
(4) TNTA-web also is introduced in the solar hydrogen production from water, which
shows a significant improvement of H2 photoproduction from suitable aqueous solutions.
(1) “Electrochemical Milling and Faceting: Size Reduction and Catalytic Activation of
Palladium Nanoparticles”, Angew. Chem. Int. Ed., Vol. 51, Issue 34, (2012) 8500-8504.
Yan-Xin Chen, Alessandro Lavacchi, Sheng-Pei Chen, Francesco di Benedetto, Manuela
Bevilacqua, Claudio Bianchini, Paolo Fornasiero, Massimo Innocenti, Marcello Marelli,
Werner Oberhauser, Shi-Gang Sun, Francesco Vizza
(2) “Energy Efficiency Enhancement of Ethanol Electrooxidation on Pd-CeO2/C in Passive
and Active Polymer Electrolyte-Membrane Fuel Cells”, ChemSusChem., Vol. 5, Issue 7,
(2012) 1266-1273. Valentina Bambagioni, Claudio Bianchini, YanXin Chen, Jonathan
23
Filippi, Paolo Fornasiero, Massimo Innocenti, Alessandro Lavacchi, Andrea Marchionni,
Werner Oberhauser, Francesco Vizza
(3) “Electrooxidation in Alkaline Media of Ethylene Glycol and Glycerol on Pd-(Ni-Zn)/C
Anodes in Direct Alcohol Fuel Cells”, (submitted to ChemSusChem in October, 2012 ) .
Andrea Marchionni, Manuela Bevilacqua, Claudio Bianchini, Yan-Xin Chen, Jonathan
Filippi, Paolo Fornasiero, Alessandro Lavacchi, Hamish Miller, Lianqin Wang, Francesco
Vizza ,
(4) “Titania Nanotueb Ordered Arrays Anodes Boost Platinum Free Direct Alcohol Fuel Cell
Performance and Stability”, (manuscript in preparation) .YanXin Chen, et.al
(1) Y.X. Chen, S.P. Chen, Z.Y. Zhou, P. Fornasiero, C. Bianchini, S.G. Sun, “ShapeControlled Synthesis of Fe Micro flowers and their Catalytic Properties for Nitrite
Reduction”, ISE2010, s07-P-063, Nice, France, 2010
(2) Y.X. Chen, P. Fornasiero, A. Lavacchi, C. Bianchini, F. Vizza, S.P. Chen, S.G. Sun,
“Electrochemical Milling and Faceting (ECMF): a New Method for Size Reduction and
Catalytic Activation of Palladium Nanoparticles”, ISACS9, P-099, Xiamen, China, 2012
(1) “Electrochemically shape-controlled synthesis of Fe nanoparticles, their structural
characterization and properties”, oral presentation, ICCOM-CNR Florence, Sesto Fiorentino,
24 March 2010
14/08/2012-19/09/2012 xiamen university, xiamen of China
(1). Nanotechnology Summer School 2011 – September 20-23, 2011, Trieste, Italy.
(2). PhD program in “Chemistry” International Course “New Concepts in Catalysis” – 24-29
June, 2012, Pavia, Italy.
(1). Annual meeting PhD School in Nanotechnology, 18-20 January 2010, total 25 hours.
(4). The 61st Annual Meeting of the International Society of Electrochemistry, September
26th - October 1st, 2010, Nice, France
24
(5). 9° Congresso del Gruppo Interdivisionale di Chimica Organometallica della Società
Chimica Italiana ( Co. G.I.C.O), 8-11 June 2010, Firenze, Italy.
(6). 9th International Symposium on Carbanion Chemistry, 20-24, July 2010, Firenze, Italy.
(7). The China-Italy Regional Cooperation Forum on Technology and Innovation. 10-12
November, 2010, Firenze, Italy
(8). “Carbohydrate recognition: Chemistry issues and applications”, Prof. Bing-He Wang.
17/05/ 2011, Firenze, Italy.
(9). “Form Metal to Metal-Free Catalysts”, Dr. Cuong Phan- Huu & Dr. Francois Garin,
29/06/2011, Firenze, Italy.
(10). “Stability diagram of dipolar Bose Einstein condensate in an optical lattice”, Dr. Alves
Emmauel De Lima Henn, 13/10/2011, Firenze, Italy.
(11). “High resolution structure and stability of gold nanoparticles/protein complex”, Dr.
Luigi Calzolai, 18/10/2011, Firenze, Italy.
(12). “Computer simulation of biomolecules”, Draio A. Estrin, 26/10/2011, Firenze, Italy.
(13). Challenges in Nanoscience, the International Symposia on Advancing the Chemical
Sciences (ISACS9), Xiamen, China, 2012– 31 Auguest - 3 September.
- Read the book《TiO2 Nanotube Arrays-Synthesis, Properties, and ApplicationsSPRINGER 2009》, 10-20 March, 2010, total 40 hours
- Read the book《Electrochemical Methods Fundamentals and Applications John Wiley
& Sons, Inc,1980》, 5-20 Auguest, 2010, total 60 hours
- Read the book《Scanning Microscopy for Nanotechnology》, 2-21 May, 2011, total 30
hours
- Read the book《Characterization of Nanophase Materials》, 10-20 Auguest, 2011, total
40 hours
- Read the book《Renewable Resources and Renewable Energy: A Global Challenge,
Second Edition》, 5-20 October, 2011, total 60 hours
- Read the book《Handbook of Electrochemistry by Cynthia G. Zoski》, 1-20 November,
2011, total 80 hours
- Read the book《 Solar Hydrogen Generation》, 2-21 June, 2012, total 70 hours
- Read the book《Fuel Cell fundamentals》, 1-20 September, 2012, total 80 hours
25
MARIO GANAU
Title of the thesis: Nanotechnology applications in quantitative neuroscience: proteomic
analyses of malignant gliomas
Supervisor: Prof Giacinto Scoles
Tutors: Dr Loredana Casalis
RESEARCH ACTIVITY
The main focus of this PhD thesis is oriented toward the proteomic analyses of astrocytes in
neuro-oncogenesis processes. A systematic review of multiple independent proteomic
analyses of gliomas has demonstrated alterations of almost 100 different proteins; the current
limit of knowledge advancement is related to the high sensitivity required to accurately
monitor protein-protein interaction to follow changes in cellular pathways due to different
kinds of external perturbations. To address this problem we have chosen to develop a
quantitative approach based on nanotechnology to eventually enable precise, high throughput
and low cost analysis of few glial cells with potential capability of real-time pathological
screening and subtyping of brain tumors. Towards this goal, we decided to develop and
characterize micro-fabricated wells capable to sort and host living cells and to optimize
immuno nano-arrays to study, at high sensitivity, the cells secretome or proteome.
The experimental activity was devoted to the fabrication and optimization of nanostructures
for protein arrays. The biomarker chosen was: Glial Fibrillary Acidic Protein (GFAP), which
belongs to the family of intermediate filaments and is crucial in cell’s differentiation. For the
fabrication of nanoarrays, meant to immobilize antibodies specific for the abovementioned
protein, an approach consisting in DNA-directed-immobilization (DDI) of biotinilated
antibodies, a nanografting technique based on Atomic Force Microscopy (AFM) has been
applied. Nanosized patches of thiol modified single strand DNA (ssDNA) were prepared by
AFM-based nanografting inside a matrix of self assembled monolayers (SAM) of alkenthiolmodified gold surfaces. Subsequently a complementary DNA strand (cDNA) conjugated to
streptavidine (STV) was allowed to covalently bind to the patch by sequence specific DNA
hybridization. Finally the biotin binding sites of STV (4 binding sites per tetramer of STV)
was exploited to immobilize biotinilated monoclonal GFAP Ab (alreafy in use for ELISA
analyses) on the top of those nanopatches. The efficiency of those nano-immuno arrays was
tested by successfully obtaining the immobilization of purified recombinant GFAP protein at
a concentration of 40 nM firstly in standard conditions then in a multicells’ proteome. The
immobilization was detected by means of AFM measuring step by step the increases in the
26
height of the patches and excluding modification of the signal to noise ratio and of the
roughness of both the SAM and the nanopatches after incubation with the proteome.
•Ganau M, Bosco A, Palma A, Beltrami AP, Cesselli D, Casalis L, Scoles G. Label-free
nano-immuno-detection of GFAP in multicells’ lysate. In preparation
•M.Ganau, P.Parisse, S.Corvaglia, L.Ianeselli, D.Scaini, C.Sanavio, L.Casalis, G.Scoles.
Proteomic analyses of malignant gliomas. Regional Summer School of
Nanotechnology 2011
•S.Corvaglia, M.Ganau, Fruk L, Sanavio B, Ianeselli L, Cesselli D, Beltrami AP, Scoles
G, Scaini D, Casalis L. Combination of novel protein nanoarrays and microwells
devices for single cell protein profiling. Gordon Research Conference Proceedings
2011
•April 29, 2010: Toffoli Giuseppe, “Pharmacokinetics and Pharmacogenomics” Facoltà di
Medicina UniTs
•Fall-Spring 2010: Giacinto Scoles, Lectures of Nanotechnology and Biochemistry
•January 11-14, 2011: Statistics Course for Medical Residents c/o UniTs
•April 11, 2011: Prof Livesey, Seminar “Stem cell models of human cerebral cortex
development and disease” c/o UniTs
•June 24, 2011: Prof Legname (SISSA), Prof Zanusso (UniVr), Prof Geschwind (UCSF)
Seminar: Molecular Biology and clinical aspects of prion diseases c/o UniTs
•July 29, 2011: Dr Castronovo (Temple University): The Effect of Confinement on
Enzymes Diffusion and Reactions Inside DNA Nanostructures c/o UniTs
•October 31-November 01, 2011: Lectures on Quantitative approaches to Biological
Problems c/o ICTP
•The experimental activity was conducted along the course of the 3 academic years at the
NanoInnovation Lab Elettra Sincrotrone Trieste
Conferences, seminars, advanced courses and other didactic activities /
•Annual Graduate School of Nanotechnology Annual Meeting c/o UniTs
•Annual Regional Summer School of Nanotechnology c/o UniT
•(2010) Hanna Mamzer, Seminars “Autopresentation and preparing presentations” and
“Comunication in cross cultural environment” c/o UniTs
27
•(2010) Giacinto Scoles, Seminar “Sapendo fare “single Cell Proteomics” come la
vendiamo a medici e biologi?” c/o UniTs
•(2010) Conference “Il ruolo emergente delle Nanotecnologie nelle Scienze della Vita”
Area Science Park
•(2010) Lucia Pasquato, Alberto Morgante and Loredana Casalis, Seminars “Molecular
self-assembling and nanostructures” c/o UniTs
•(2010) Sangeeta Kale, Seminar “ Metal oxide nanomaterials in sensors and biomedicine”
c/o UniTs
•(2011) Seminar BioMol c/o Elettra
•(2011) Seminar Brain Awareness Week c/o Centro Interdipartimentale per le
Neuroscienze Brain
•(2011) Joint ICTP-KFAS conference on Nanotechnology for Biological and Biomedical
Applications (Nano-Bio-Med) c/o ICTP
•(2011) Conference on System Biology and New Sequencing Techniques c/o ICTP
28
VALENTINA GIORGIS
Title of the thesis: Design, fabrication and characterization of metamaterials inspired
plasmonic structures for sensing application
Supervisor: Filippo Romanato

RESEARCH ACTIVITY
My research is focused on design, fabrication and characterization of metamaterials inspired
plasmonic structures, expecially on Split Ring Resonator (SRR) geometry. The finite element
analysis software COMSOL is used to design the metamaterials, with the support of LaNN
group theoreticians (Padua). Samples are fabricated with X-Ray Lithography (XRL)
technique and Electron Beam Lithography (EBL), then grown in gold using electochemical
bath. To support samole fabrication other techniques are used: wet and dry etching
(membrane production for X-Ray mask), metal evaporation deposition (base plating for a
non-conductive substrate), sputtering deposition (substrate fabrication). Positive and negative
resist has been used in order to reply mask pattern on the substrate with XRL. The final goal
is the fabrication of structures to study as bio-chemical sensor. During the last year we design
and fabricate new X-ray mask geometries and the litographic processes have been optimized.
At the moment large area samples for microfluidic analysis have been fabricated on a
transparent substrate (ITO on glass) to achieve transmission measurement. Sample
characterization has shown a red-shif of the plasmon resonance peack when the structure is
functionalized with thiols.
Beside the thesis project, I collaborate to various research project in my group, fabricating
samples (electochemical growt, evaporation deposition) and designing and building new
facilities. I collaborate, also, to external universities and companies projects.
•
Sammito, G. Zacco, P. Zilio, V. Giorgis, A. Martucci, J. Janusonis, F. Romanato, “Design and
fabrication of a light trapping method for photovoltaic devices based on plasmonic gratings”,
Microelectronic Engineering 98 , pp. 440-443
F. Romanato, M. Massari, T. Ongarello, M. Carli, G. Pirruccio, D. Sammito, V. Giorgis, D.
Garoli, R. Bozio, R. Pilot, R. Signorini, P. Schiavuta, F. Marinello, “Design, Fabrication and
characterization of Plasmonic Gratings for SERS”, Microelectronic Engineering 88 (8), pp.
2717-2720
D. Garoli, M. Natali, G. Parisi, T. Ongarello, E. Sovernigo, M. Massari, V. Giorgis, G.
Ruffato, S. De Zuani, F. Romanato “Fabrication of metamaterials in the optical spectral
range”, Microelectronic Engineering 88 (8), pp. 1951-1954
29
•
Publications/abstracts in conferences/congresses (national or
international)
V. Giorgis, M. Massari, P. Zilio, G. Parisi, G. Ruffato, G. Grenci, F. Romanato, “Large area
array of split ring resonators for sensing applications ”, Metamaterials 2012
V. Giorgis, F. Romanato, M. Massari, G. Parisi, M. Carli, D. Sammito, E. Sovernigo,
“Design, Fabrication and Characterization of Split Ring Resonators using X-Ray
Lithography”, MNE 2011
G. Bovo, D. Sammito, D. De Salvador, G. Biasiol, M. Gaio, T. Ongarello, V. Giorgis, “Design
of a plasmonic structure integrated on a GaAs HEMT photodetector for biosensing
applications”, MNE 2011
D. Sammito, G. Zacco, P. Zilio, V. Giorgis, A. Martucci, J. Janusonis, “Design and fabrication
of plasmonic gratings for bulk Silicon solar cell light harvesting enhancement”, MNE 2011
S. De Zuani, M. Natali, D. Garoli, V. Giorgis, M. Massari, G. Parisi, “Ferroelectric
metamaterials: towards a refractive index control”, Metamaterials 2011
•
Other publications
Sammito, P. Zilio, G. Zacco, V. Giorgis, F. Romanato, “Nanotrutture plasmoniche per la
raccolta della luce, PV Technology, 4/4/2010, p,46

•
Metamaterials 2011, St Petersburb, Russia, 17/09/2012-20/09/2012
Nanotechnology Summer School 2012, Udine, Italia, 2/07/2011-5/07/2011
2012 Winter College on Optics, Trieste, Italia, 6/2/2012-17/2/2012
Nanotechnology Summer School 2011, Trieste, Italia, 20/09/2011-23/09/2011
Metamaterials 2011, Barcellona, Spagna, 10/10/2011-13/10/2011
Metamaterials Doctoral School, Barcellona, Spagna, 14/10/2011-15/10/2011
30
MARIANNA LUCAFÒ
Title of the thesis: CARBON NANOSTRUCTURES AS NANOCARRIERS FOR
ANTITUMORAL DRUGS
Supervisor: Prof. Gianni Sava
Tutors: Dr. Sabrina Pacor, Dr. Tatiana Da Ros, Prof. Sonia Zorzet
RESEARCH ACTIVITY
The study of carbon nanostructures, such as fullerene, is of strong interest in biomedical field
also thanks to the possibility of using them as vectors in drug delivery. During these three
years, in vitro studies were carried out on two fullerene derivatives (F2 and F3) using
different cell lines with the purpose to evaluate whether cytotoxicity of these compounds
could change depending on the cell lines used. The studies have allowed to spot one fullerene,
which has shown a very limited toxicity (F2) and another with high toxicity (F3).
Unfortunately many of these studies show contradictory and ambiguous results, pointing out
that the molecular mechanisms underlying the cytotoxicity of these nanomaterials are not yet
completely understood. On the basis of this knowledge and with the aim to address the cell
toxicity of these compounds, we performed a whole genome expression analysis, by high
throughput RNA sequencing, on a human adenocarcinoma cell line exposed to two fullerenes,
using the Illumina technology. Our results show that the treatment with the two fullerenes
induces similar gene expression changes in a time dependent fashion. These changes include a
general depression of processes related to transcription and protein synthesis, determining a
slowdown of the cell cycle progression. The intensity of the alterations observed in response
to F3 was always higher than for F2, confirming the previously data. Therefore F2 was
chosen to continue the study as a suitable nanocarrier. Uptake tests were made on MCF7 cell
line up to 72 hours, time in which the fluorescence value of F2 seems to remain stable.
Fluorescence microscopy techniques have demonstrated an absence of co-localization both in
mitochondria and lysosomes after treatment with F2. Moreover, it was seen that F2 did not
co-localized inside the nuclei; these data are important as they exclude the possibility of direct
interaction between F2 and DNA. Experiments were made on conjugated F2DOXORUBICIN above all to verify if the activity of the drug is retained or enhanced when
linked to the fullerene. Furthermore we studied the cellular uptake of F2-DOXO in both
MCF7 and MCF/ADR lines. Cytotoxicity tests have shown that F2-DOXO has an irrelevant
activity compared to free drug because doxorubicin cannot get into the nucleus to perform its
activity as it remains linked to F2. Nevertheless, the internalization of F2-DOXO is higher in
MCF7/ADR compared to the free drug. So F2 could be a suitable vector to avoid the “Multidrug resistance” phenomenon caused by P-gp extrusion pump.
31
Lucafò M., Gerdol M., Pallavicini A., Pacor S., Zorzet S., Da Ros T., Prato M., Sava G.
“Profiling the molecular mechanism of fullerene cytotoxicity on tumor cells by RNA-seq”
ACS NANO (Submitted);
Lucafò M., Pacor S., Fabbro C., Da Ros T., Zorzet S., Prato M. and Sava G. “Study of a
potential drug delivery system based on carbon nanoparticles: effects of fullerene derivatives
in MCF7 mammary carcinoma cells” Journal of Nanoparticle Research, vol. 14, pp. 830-842
(ISSN 1388-0764), 2012
16-19 September 2012, Rimini – 16° Seminario Nazionale SIF Dottorandi ed Assegnisti di
Ricerca
02-05 July 2012 -Udine- Nanotechnology Summer School (Poster)
27-30 March 2011, Trieste - 5° Meeting Nuove Prospettive in Chimica Farmaceutica
(Abstract and Poster);
14-17 September 2011 – Bologna - 35° Congresso Nazionale Della Società Italiana di
Farmacologia (Abstract and poster)
20-23 September 2010 - Certosa di Pontignano (Siena) - “XIV Seminario nazionale per
dottorandi in Farmacologia e Scienze affini” (Abstract and Poster)
Conference 20-22 October 2010 - Mestre (VE) - NanotechItaly 2010 (Abstract and poster)
Congress 24 November 2010 – Milano - Nanotecnologie e veicolazione di Farmaci (AICC)
(poster co-author)
16-19 September 2012, Rimini – 16° Seminario Nazionale SIF Dottorandi ed Assegnisti di
Ricerca
27\10\2010 al 29\10\2010, (30 h) Lectures on Pharmacology, given by Prof. Gianni Sava, for
the students of the degree in Chemistry and pharmaceutics technology in the Faculty of
Pharmacy;
Lectures on the use of flow cytometer by Dr. Sabrina Pacor since February 2010 (about 40
hrs).
10-14 September 2012 – Udine- Biology, Computation and Information Summer School
20-23 September 2011 -Trieste- Nanotechnology Summer School 2011
32
20-23 September 2010 -Certosa di Pontignano (Siena)- XIV Seminario nazionale per
dottorandi in Farmacologia e Scienze affini
Tutor for the theses in the Faculty of Pharmacy, titled:
- “Confronto della citotossicità di fullereni funzionalizzati quali potenziali vettori per
farmaci” (Supervisor Dr. Sabrina Pacor)
- “Valutazione di biocompatibilità di nanoparticelle (SPION, GNPs) in cellule tumorali”
(Supervisor Dr. Sabrina Pacor)
- “Nanovettori per farmaci antitumorali: studio di un fullerene funzionalizzato ed analisi
preliminare del coniugato con la doxorubicina” (Supervisor Dr. Sabrina Pacor)
- “Studio di fullereni funzionalizzati come vettori per farmaci” (Supervisor Dr. Sabrina Pacor)
-“Studio pilota su un modello di linfoma umano dell’attività antitumorale di nanoparticelle
veicolate specificatamente” (Supervisor Prof. Sonia Zorzet);
I worked as a tutor during lessons for high school lab (LLC).
LUCIA MARSICH
Title of the thesis: “Design and synthesis of functionalized metal nanoparticles for bioanalysis with Surface-Enhanced Raman Scattering (SERS)”
Supervisor: Valter Sergo
Tutors: Alois Bonifacio
RESEARCH ACTIVITY
During the first year as a PhD student, I developed a SERS-active substrate made of citrate
reduced silver nanoparticles coated with poly-L-lysine (PLL-AgNPs). This substrate was
applied for measuring free bilirubin. During my second year a calibration curve was done,
confidence bands, limit of quantitation and of detection were calculated with the purpose of
quantifying bilirubin in samples with unknown concentration.
To apply the PLL-AgNPs for quantifying bilirubin uptake of cellular cultures exposed to
nanomolar concentration of bilirubin a new buffer solution, suitable for cell growth and SERS
measurement, were prepared. In particular the osmolarity and the pH were chosen considering
the cell growth. With this new buffer, the bilirubin spectrum intensity is lower, because of the
different protonation state of the PLL. In order to substitute the PLL coating, two polymer
with quaternary nitrogen atom were chosen.
The coated nanoparticles absorption maximum shown a red shift of the plasmonic frequency
indicating that the coating succeeded. The relatively small red shift rules out the formation of
large nanoparticles aggregates. The nanoparticles coated with this two polymer were applied
to detect nanomolar concentration of bilirubin.
33
The interaction between the PLL-AgNPs and the albumin was studied. TEM images of
albumin added to the PLL-AgNPs showed the protein bonded on the PLL layer around NPs.
Since the bilirubin is itself hydrophobic, the NPs were coated with an hydrophobic and redisperse in hexane.
During the period at the Technical University in Berlin (prof. Hildebrandt’s group) I have
developed chitosan coated silver nanoparticles to detect the cytocrome-c attached on a gold
surface and silica coated silver nanoparticles to detect cytochrome-b5 attached on a gold
surface.
•Marsich, L., Moimas, L., Sergo, V., Schmid, C.; “Raman spectroscopic study of
bioactive silica-based glasses: The role of the alkali/alkali earth ratio on the NonBridging Oxygen/Bridging Oxygen (NBO/BO) ratio”; Spectroscopy 23, 2009; pp 227232
•Marsich L, Bonifacio A, Mandal S, Krol S, Beleites C, and Sergo V, “Poly-L-lysine
coated silver nanoparticles as positively charged substrates for Surface Enhanced
Raman Scattering”, Langmuir, 2012, 28, 13166−13171.
•In preparation: Bonifacio A, Barbone M, Marsich L, Lughi V, Sergo V, “SurfaceEnhanced Raman Effect in Hybrid Metal-Semiconductor Nanoparticle Assemblies”
Poster
•“Polylysine coated silver nanoparticles as sensors for bilirubin quantification using
Surface Enhanced Raman Spectroscopy (SERS)”, L. Marsich, A. Bonifacio, V. Sergo;
Yellow Retreat; Trieste, 06 – 07 June 2011 (Congress about bilirubin studies
organized by Centro Studi Fegato)
•“Coated Silver Nanoparticles to Detect Heme Proteins on Gold Electrodes”, L. Marsich,
A. Bonifacio, I. Weidinger, P. Hildebrandt, V. Sergo, Regional summer school of
Nanotechnology, Udine, 2-5 July 2012
Since the 24th of October 2011 till 19th of Aprile 2012 in Berlin (Germany) at “Technische
Universitaet”, in the “Institut fuer Chemie Max-Volmer-Laboratorium für Biophysikalische
Chemie”.
Classes followed (substituted by personal study)
Books:
•R. Aroca, “Surface-Enhanced Vibrational Spectroscopy” 2006 John Wiley & Sons, Ltd
34
•“Bile pigments and Jaudice , Edited by J. Donald Ostrow. New York: Marcel Dekker,
Inc., 1986.
•D. Harvey, “Modern Analytical Chemistry”, 2000 McGraw-Hill Higher Education
•F. Siebert and P. Hildebrant, “Vibrational spectroscopy in Life Science”, 2008, WILEYVCH
Reviews:
•Hyunhyub Ko, Srikanth Singamaneni, and Vladimir V. Tsukruk, ”Nanostructured
Surfaces and Assemblies as SERS Media”, Small 2008, 4, No. 10, 1576–1599
•Richard J. C. Brown, Martin J. T. Milton, “Nanostructures and nanostructured substrates
for surface-enhanced Raman scattering (SERS)”, J. Raman Spectrosc. 2008; 39:
1313–1326
•Xiu-Mei Lin, Yan Cui, Yan-Hui Xu, Bin Ren, Zhong-Qun Tian, “Surface-enhanced
Raman spectroscopy: substrate-related issues”, Anal Bioanal Chem, DOI
10.1007/s00216-009-2761-5
•J. Millstone et al. “Colloidal gold and silver triangular nanoprisms.”, Small, 2009, Vol 5,
Issue 6, Pages 646-664
•A. Guerrero Martinez “Nanostars shine bright for you: Colloidal synthesis, properties
and applications of branched metallic nanoparticles” Current Opinion in Colloid &
Interface Science, April 2011, Volume 16, Issue 2, Pages 118-127.
Final Conference 2009 – Trieste,18 - 20 January 2010 (PhD School in Nanotechnology)
How to present scientific results, Trieste 22 January 2010
Yellow Retreat – Trieste, 08 – 09 March 2010 Yellow Reatreat – Trieste, 08 – 09 March 2010
(Congress about bilirubin studies organized by Centro Studi Fegato)
Intensive course about "Molecular self-assembling and nanostructures” , Trieste 23 June - 13
July 2010
Tour of SENIL laboratories (Elettra) - Trieste, 20 July 2010
XVI Scuola Nazionale di Scienza dei Materiali – Bressanone (BZ), 27 September- 02 October
2010
“High-Resolution Integrated Micro Electrode Arrays (MEAs) for Imaging
Neurophysiological Signaling” – Trieste, 25 October 2010
“Introduzione alla tecnologia MEMS”, Dr Ing Cristina Bertoni – Trieste, 12 April
“Synthesis and structure elucidation of kaolinite organo-complexes”, János Kristóf and
Erzsébet Horváth – Trieste, 3 May 2011
Yellow Retreat – Trieste, 06 – 07 June 2011 (Congress about bilirubin studies organized by
Centro Studi Fegato)
“Self assembled monolayers on gold the challenge”, Dr. Hicham Hmamoudi - 1 July 2011
35
“The Effect of Confinement on Enzymes Diffusion and Reactions Inside DNA
Nanostructures”, Matteo Castronovo – 18 July 2011
Regional summer school of Nanotechnology – Trieste, 19 – 23 September 2011
“Functionalized nanoparticles for bioanalysis”, Arumugam Sivanesan, Berlin, 13 January
2012
“Influence of the protein environment on the Raman spectra of cyanobacterial phytochrome”
Grazia Daminelli, Berlin, 20 January 2012.
“The investigation of rubber by vibrational spectra”, Katharina Haider, Berlin, 9 March 2012
“SERS for protein and estrogen detection”, XiaoXia Han, Berlin, 20 April 2012
Regional summer school of Nanotechnology, Udine, 2-5 July 2012
“Robust Imaging and Delivery Systems: Targeted Nanoparticles”, dr. Luis Nunez, Universtiy
of Chicago and BioTarget Inc, Trieste, 17 September 2012
20 h following three students in the Raman laboratory.
Safety training of undergraduate students in chemical laboratory.
Co-advisor of a master thesis in material engineering with the title: “Sintesi e
caratterizzazione di nanoparticelle ibride semiconduttore-metallo come marcatori ottici per
applicazioni biomediche”.
Co-advisor of a bachelor thesis in industrial engineering with the title: “Nanostrutture
metalliche per applicazioni SERS (Surface Enhanced Raman Spectroscopy) ottenute per
deposizione di oro”.
Co-advisor of a bachelor thesis in industrial engineering with the title: “Valutazione dei rischi
e pianificazione del sistema di prevenzione e protezione: applicazione ad un laboratorio
chimico universitario a scopo didattico e di ricerca”.
36
ANGELO PERONIO
Title of the thesis: Single-molecule heterogeneous catalysis
Supervisor: prof. Giovanni Comelli
Tutors: prof. Carlo Dri
RESEARCH ACTIVITY
My research topic is the study of model systems for heterogeneous catalysis at the singlemolecule level by means of sub-molecularly resolved imaging and spectroscopy with a lowtemperature scanning tunnelling microscope (LT-STM).
In these years I studied a coadsorption complex formed by ammonia and nitrogen monoxide
on the (111) surface of platinum. This is a model system to understand the selectivity in the
catalytic reduction of nitrogen monoxide by ammonia (SCR). I determined the microscopic
structure of the complex and the nature of the interaction between NH3 and NO, using a
combined experimental (STM) and theoretical (DFT) approach.
A side topic was the hydrogenation of CO2 to methanol catalyzed by nickel and copper alloys.
In particular I characterized by STM the structural properties of CO2 chemisorbed on Ni(110).
This species is particularly reactive, due to a strong charge transfer from the surface, and has
thus a central role in the hydrogenation process.
On the instrumental side. I introduced in our laboratory the inelastic electron tunneling
spectroscopy (STM-IETS), which allows to measure the vibrational spectrum of single
molecules adsorbed on a surface. Since our group lacked previous expertise in STM-based
spectroscopies, it was necessary to understand in detail the role of the various experimental
parameters. This characterization led to the formulation of a set of protocols to measure the
performance of the various components of a STM system, and to an innovative detection
scheme, increasing the energy-resolution of the IETS spectra.
•C. Dri, A. Peronio, E. Vesselli, C. Africh, M. Rizzi, A. Baldereschi, M. Peressi, and G.
Comelli, Imaging and characterization of activated CO2 species on Ni(110)
Phys. Rev. B 82, 165403 (1-6) (2010), doi: 10.1103/PhysRevB.82.165403
•M. Rizzi, S. Furlan, M. Peressi, A. Baldereschi, C. Dri, A. Peronio, C. Africh, P. Lacovig, E.
Vesselli, and G. Comelli Tailoring bimetallic alloy surface properties by kinetic control of
self-diffusion processes at the nano-scale
J. Am. Chem. Soc. 134 (2012) 16827, doi: 10.1021/ja307294p
37
•Poster: CO2 activation and hydrogenation on Ni model catalysts: an atomic-scale investigation
by spectroscopy and microscopy measurements and ab-initio calculations; E. Vesselli, M.
Rizzi, C. Dri, A. Peronio, C. Africh, X. Ding, A. Baraldi, A.Baldereschi, M. Peressi, and G.
Comelli, presented at the workshop of the Istituto Officina dei Materiali del CNR, 30
settembre – Padriciano (TS), October 1, 2010,
•Poster STM-based tools for the investigation of chemical processes at the atomic level: FastSTM
and single molecule vibrational spectroscopies; C.Dri, A.Peronio, C.Africh, F.Esch and
G.Comelli, presented at the workshop of the Istituto Officina dei Materiali del CNR, 30
settembre – Padriciano (TS), October 1, 2010
•Talk Catalysis at the single molecule level at the annual meeting of the School in
Nanotechnology, University of Trieste, January 17-19, 2011
•Poster A NH3‒NO coadsorption complex on Pt(111), A. Peronio, A. Cepellotti, S. Marchini, N.
Abdurakhmanova, C. Dri, C. Africh, F. Esch, M. Peressi e G. Comelli, presented at the 1st
joint Summer School on Nanotechnology, University of Trieste, September 20-23 2011; and at
the Slonano 2011 conference, Institut Jožef Stefan, Ljubljana (SLO), October 27, 2011
•Talk Catalysis at the single molecule level at the annual meeting of the School in
Nanotechnology, University of Trieste, January 16-18, 2012
•Poster Ideas towards an optimized detection scheme for STM-IETS, A. Peronio, C. Dri e G.
Comelli, presented at the 2nd joint summer school on Nanotechnology, Udine, July 2-5 2012
•Talk Optimized detection scheme for STM-IETS at the 14a Vibrations At Surfaces conference,
Kobe (JP), September 24-28, 2012
•Annual meeting of the Graduate school of Nanotechnology, University of Trieste, January 17-19,
2011. Talk Catalysis at the single molecule level
•Annual meeting of the Graduate school of Nanotechnology, University of Trieste, January 16-18,
2012. Talk Catalysis at the single molecule level
a
•14 Vibrations At Surfaces conference, Kobe (JP), September 24-28, 2012. Talk Optimized
detection scheme for STM-IETS
November 7, 2011 – December 22, 2011: visiting scientist at prof. Saw-Wai Hla group, Ohio
University, Athens (OH), USA
38
•Heterogeneous catalysis, module of M.Sc. programme in Chemistry at Trieste University, taught
by prof. Paolo Fornasiero, 16 hours, March 2010.
•Advanced statistics for data analysis, course of
M.Sc. programme in Physics at Trieste
University, taught by prof. Edoardo Milotti, 48 hours, ottobre-dicembre 2010.
•Photoemission spectroscopies and spectromicroscopies, course of PhD programme in Physics at
Trieste University, taught by proff. Alessandro Baraldi, Andrea Goldoni and Andrea Locatelli,
25 hours, November-December 2010
•Molecular self-assembling and nanostructures, course of the graduate school in Nanotechnology
of the University of Trieste, taught by dr. Loredana Casalis, prof. Alberto Morgante, and prof.
Lucia Pasquato, 10 hours followed, June 21, 2011 – July 15, 2011
•Laboratory: almost all of my research activity was performed at the SSR-STM laboratory of
IOM-CNR laboratorio Tasc.
•School Synchrotron and free-electron-laser sources and their multidisciplinary applications,
Abdus Salam International Centre for Theoretical Physics, Trieste, about 70 hours, April 26 –
May 7 2010
•Spring college on Computational nanoscience, Abdus Salam International Centre for Theoretical
Physics, Trieste, about 70 hours, May 17 - 28 2010.
•Workshop of the Istituto Officina dei Materiali of CNR, Padriciano (TS), about 12 ore,
September 30 – October 1 2010
•Seminar Synthesis and structure elucidation of kaolinite organo-complexes by prof.
János Kristóf and Erzsébet Horváth of the Universiy of Pannonia, University of
Trieste, May 3 2011
•Seminar Self assembled monolayers on gold the challenge by dr. Hicham Hamoudi of the
Université-Paris Sud, University of Trieste, July 1, 2011
a
•1 joint Summer School on Nanotechnology, University of Trieste, September 20-23,
2011
•Slonano 2011 conference, Institut Jožef Stefan, Ljubljana (SLO), October 27, 2011
•Spring course on Diffraction Methods for Nanostructured Materials. Taught by prof. Norberto
Masciocchi from Unversity of Insubria and Antonietta Guagliardi from Istituto di
Cristallografia of CNR and University of Insubria. University of Trieste, March 15, 2012.
•Seminar How to perform a literature search using the resources available at UNITS, and how to
connect to the UNITS network form outside the campus, by prof. Maurizio Fermeglia of the
University of Trieste. University of Trieste, June 6, 2012.
•Seminar Polymer-based nanocomposites, by dr. Galder Kortaberria from Escuela Universitaria
Politécnica of Donostia (ES). University of Trieste, June 6, 2012.
39
•2a joint summer school on Nanotechnology, July 2-5 2012, Udine.
•14a Vibrations At Surfaces conference, September 24-28 2012, Kobe (JP).
•Tutor for a B.Sc thesis in Physics, Vibrational properties of single molecules of ammonia
adsorbed on Pt(111) by low-temperature scanning tunneling microscopy, University of
Trieste, defended on September 24, 2010.
•April 4 2011, January 23 2012 and February 15 2012: hands-on seminar for high school students
“The physics of hydrogen fuel cells” at the department of Physics of the University of Trieste,
within the Progetto Lauree Scientifiche of the University of Trieste. 1st place at the Italian
national contest “Piano Lauree Scientifiche” for the best popular work in Chemistry for high
school students
•Tutor for a M.Sc thesis in Physics, Structural and electronic properties of cysteamine adsorbed
on gold surface by low-temperature scanning tunneling microscopy, University of Milano, to
be defended.
40
DAVIDE SAMMITO
Title of the thesis: Integration of plasmonic gratings into optoelectronic devices
Supervisor: prof. Filippo Romanato
RESEARCH ACTIVITY
My research activity is focused on the integration of nanostructured metallic gratings on
optoelectronic devices with the aim to exploit plasmonic effects to control light absorption
properties. I have been working on three different applications of plasmonic crystals to thin
film organic solar cells, to wafer-based conventional silicon solar cells and to phototransistor
made of GaAs/AlGaAs. In particular during the last year I have been focusing on the last two
applications.
For what concerns photovoltaic devices, I wrote three papers. I have compared, in thin film
and wafer-based solar cells, the light trapping properties of gratings constituted by silver
nanowires. I described the nanofabrication technique developed, that is based on laser
interference lithography and is suited to the large surface area of solar cells. I highlighted by
simulations that the designed gratings allow a global conversion efficiency enhancement in
thin film devices. On the other hand in wafer-based cells only an improvement of internal
quantum efficiency can be obtained and it is due to the redistribution of light absorption
profile in the semiconductor. These findings are supported by comparison of modeling results
with external quantum efficiency and front surface reflectance measurements.
For what concerns phototransistors, I have studied the integration of gold plasmonic gratings
with V-groove shape on the gate. The aim is to detect, by variations in conductivity of the
devices, modulations of light intensity transmitted toward the 2D electron gas due to the
coating of the gate surface with a thin layer of bioanalytes. I have optimized the epitaxial
multilayer measuring the electro-optical response and fabricated devices by lithographic
techniques. In a first batch of samples, the trend of conductivity versus incidence angle
curves, before and after functionalization, has shown a good agreement with simulation and it
is possible to estimate that a batch integrating optimized nanostructures could give a
resolution of the order of 10-5 in terms of effective refractive index.
•Sammito D., Zilio P., Zacco G., Janusonis J., Romanato F., “Light trapping properties of
metallic gratings on wafer-based silicon solar cells” (2012) Nano Energy,
http://dx.doi.org/10.1016/j.nanoen.2012.10.008
41
• Sammito D. , Zacco G. , Zilio P. ,Giorgis V. , Martucci A. , Janusonis J. , Romanato F.,
“Design and fabrication of a light trapping method for photovoltaic devices based on
plasmonic gratings” (2012) Microelectronic Engineering, 98, pp. 440–443
• Zilio P., Sammito D., Zacco G., Mazzeo M., Gigli G., Romanato F., “Light absorption
enhancement in heterostructure organic solar cells through the integration of 1-D
plasmonic gratings” (2012) Optics Express, 20(14), pp. A476-A488
• Garoli D., Romanato F., Zilio P., Natali M., Marinello F., Ongarello T., Sammito D., De
Salvador D., “Fabrication of "nano-rocket-tips" for plasmonic nanofocusing” (2011)
Microelectronic Engineering, 88 (8), pp. 2530-2532.
• Romanato F., Pilot R., Massari M., Ongarello T., Pirruccio G., Zilio P., Ruffato G., Carli
M., Sammito D., Giorgis V., Garoli D., Signorini R., Schiavuta P., Bozio R., “Design,
fabrication and characterization of plasmonic gratings for SERS” (2011)
Microelectronic Engineering, 88 (8), pp. 2717-2720.
• Zacco G., Romanato F., Sonato A., Sammito D., Ruffato G., Morpurgo M., Silvestri D.,
Carli M., Schiavuta P., Brusatin G., “Sinusoidal plasmonic crystals for bio-detection
sensors” (2011) Microelectronic Engineering, 88 (8), pp. 1898-1901.
• Zilio P., Sammito D., Zacco G., Romanato F., “Absorption profile modulation by means
of 1D digital plasmonic gratings” (2010) Optics Express, 18 (19), pp. 19558-19565.
•Poster: Sammito D., De Salvador D., Biasiol G., Bovo G., Zilio P., Ongarello T., Massari
M. and Romanato F., “Design of a AlGaAs/GaAs HEMT photodetector integrating
plasmonic nanostructures for biosensing applications”, NanotechItaly 2012, Venice
21-22 November 2012
•Poster: Zilio P., Sammito D., Zacco G., Mazzeo M., Gigli G. and Romanato F.,
“Analysis of absorption enhancement mechanisms in organic solar cells by means of
plasmonic crystals”, NanotechItaly 2012, Venice 21-22 November 2012
•Poster: Sammito D., Zacco G., Zilio P., Sovernigo E., Dai Prè M., Martucci A.,
Janusonis J. and Romanato F., “Integration of plasmonic gratings on flat wafer-based
silicon solar cells for light trapping purpose”, E-MRS spring 2012, Strasbourg 14-18
May 2012
•Poster: D. Sammito, P. Zilio, G. Zacco, A. Martucci, M. Dai Prè, J. Janusonis, J. Ulbikas,
S. Padovani, F. Rasello, S. Sinesi and F. Romanato, “The ORION project:
nanotechnology applied to bulk Silicon solar cells”, NanotechItaly 2011, Venice 23-25
November 2011
•Poster and Conference Proceedings: G. Zacco, D. Sammito, P. Zilio, G. Melcarne, G.
Gigli, M. Mazzeo, F. Romanato, “Light harvesting enhancement in organic solar cells
42
through the integration of plasmonic crystals”, 26th European Photovoltaic Solar
Energy Conference and Exhibition, Hamburg 5-9 September 2011
•Poster: G. Bovo, D. Sammito, D. De Salvador, G. Biasiol, M. Gaio, V. Giorgis, P. Zilio,
T. Ongarello and F. Romanato, “Design of a plasmonic structure integrated on a GaAs
HEMT photodetector for biosensing applications”, 37th International Conference on
Micro and Nano Engineering, Berlin 19-23 September 2011
•Poster: V. Giorgis, F. Romanato, M. Massari, G. Parisi, M. Carli, D. Sammito, E.
Sovernigo, “Design, Fabrication and Characterization of Split Ring Resonators using
X-Ray Lithography”, 37th International Conference on Micro and Nano Engineering,
Berlin 19-23 September 2011
• Poster: P. Zilio, D. Sammito, G. Zacco, and F. Romanato, “Role of Resonances of
Digital Plasmonic Gratings in Absorption Profile Remodulation in Silicon Solar
Cells”, OSA Optical Nanostructures for Photovoltaics, Karlsruhe 2010, and CNRIOM Workshop, Trieste 2010
• Poster e conference proceedings: “Absorption Profile Remodulation in Silicon Solar
Cells by Means of Plasmonic Gratings”, D. Sammito, P. Zilio, G. Zacco, and F.
Romanato, 25th EUPVSEC - WCPEC-5, Valencia 2010
Other publications
•D. Sammito, P. Zilio, G. Zacco, V. Giorgis, F. Romanato, “Nanostrutture plasmoniche
per la raccolta della luce”, PV Technology, 4/4 2010, p. 46
•“Design and fabrication of plasmonic gratings for bulk Silicon solar cell light harvesting
enhancement”, 37th International Conference on Micro and Nano Engineering, Berlin
19-23 September 2011
o“Materiali nanostrutturati”, prof. V. Lughi, “corso di laurea specialistica in ingegneria
dei materiali”, second semester 2009/2010
o“Laboratorio di materiali elettrici e fotovoltaici”, prof. A. Massi Pavan, “corso di laurea
specialistica in ingegneria dei materiali”, second semester 2009/2010
•Nanotechnology summer school, University of Udine, 2-5 July 2012
•Winter College on Optics: Advances in Nano-Optics and Plasmonics, ICTP Trieste, 6-17
February 2012
•Nanotechnology school annual meeting, University of Trieste, 16-18 January 2012
43
•Congress: “Nanotechnology school congress”, Università di Trieste 17-19 January 2011
•Course on Labview programming environment at Sincrotrone Trieste April 2011
•Course: “Molecular self-assembling and nanostructures”, Università di Trieste June-July
2011
•Seminar: “Self assembled monolayers on gold the challenge”, Hicham Hmamoudi at
Università di Trieste 1 July 2011
•Seminar: “The Effect of Confinement on Enzymes Diffusion and Reactions Inside DNA
Nanostructures”, Matteo Castronovo at Università di Trieste 29 July 2011
•Summer school: “International Summer School on Photovoltaics and New Concepts of
Quantum Solar Energy Conversion”, organized by Helmholtz Zentrum Berlin at
Hirschegg (Austria) 11-18 September 2011
•Workshop of the School of Nanotechnology: January 18th-20th 2010
•Seminar: ‘How to present scientific results in public’, dr. Hanna Mamzer of Adam
Mickiewicz University in Poznan (Poland), January 22nd 2010
•Workshop: Electron Beam Lithography with Jeol JBX-6300FS, January 25th-29th 2010 at
Nanofab, Venezia – Marghera
•Workshop: Comsol Multiphysics, 4/3/10 Trieste
•Summer school: International school of quantum electronics, 47th course advances on
nanophotonics III: plasmonics and energy efficiency, 11-18/07/10 Erice, Ettore
Majorana Foundation and Centre for Scientific Culture
•Seminar: “Development of On-line Monitoring devices for the extrusion process”, prof.
Miguel Nobrega of the University of Minho, 15/06/10
•Seminar: “Use of VPN and UGOV”, 15/06/10
•Workshop IOM, 30/09 – 1/10/2010
•Course: “Fundamentals of electron microscopy and analytical techniques”, Dr. R.
Ciancio, 12-13/10/10 Laboratorio TASC
•Seminar: “Metal oxide nanomaterials in sensors and biomedicine”, Dr. Kale of the
Defence Institute of Advanced Technology (DIAT) India, 23/11/10
•Books reading:
o “Plasmonics – fundamentals and applications”, S. Maier
o “Handbook of photovoltaic science and engineering”, A. Luque, S. Hegedus
•Tutor of Gianluca Bovo in his experimental activity finalized with the master thesis in
Material Science at University of Padua having as title “Multilayer electro-plasmonic
nanostructures for biosensing architectures”
44
CHIARA ZANUSSO
Title of the thesis: “Nanotechnologies and oncology: pharmacokinetics and
pharmacogenomics to optimize the antitumoral therapies”
Supervisor: dr. Giuseppe Toffoli
Tutors: prof. Tullio Giraldi
RESEARCH ACTIVITY
1° year: This study purpose is to characterize pharmacogenetic markers above all the
neurological type involved in the response and toxicity to the pharmacologic treatment with
oxaliplatin in patients with colorectal cancer. At first literature search has been done to define
the genetic polymorphisms of possible prognostic/predictive significance. Polymorphisms of
glutathione S-transferase (GST) has been identified, transmembrane transporters (ATPBinding Cassette) that are responsible for the extrusion of the drug from the cell, and
polymorphisms concerning DNA repair genes that codify for proteins involved in the repair
of the damage exerted on nucleic acid from mutagens as alkylating drugs.
154 patients were enrolled with colorectal cancer (CCR) treated with adjuvant FOLFOX-4
from the National Cancer Institute of Aviano (CRO) and other centres of Veneto region. At
first genomic DNA was extracted from peripheral blood samples and then the development of
analytical methods was performed. Specifically an innovative use of nanotechnologies in gene
sequencing has been implemented, such as Pyrosequencing, a technique of recent
development, that allows to identify simply and quickly mutations of punctiform type (SNP).
Finally statistical analysis of data was performed to evaluate the relative risk (OR) of
developing colorectal cancer by means of case-control studies and the role of individual gene
variations in making prone the patient to the development of toxic reactions after
chemotherapy with oxaliplatin.
2° year: The aim of this study is to assess the impact of certain genetic variants
(polymorphisms) involved in the process of carcinogenesis and tumor progression. Prostate
cancer (CA) is the third most common cause of cancer deaths in men of all ages and is a
major health problem worldwide. Incidence and mortality rates of prostate cancer vary
substantially worldwide, suggesting the importance of environmental/lifestyle risk factors and
perhaps their combination with genetic variants across racial/ethnic populations. The genetic
characteristics of the patient are one of the determinants in the inter-subject variation observed
in the outcome of anti-tumor therapy, and pharmacogenetics aims to validate and use in
clinical practice certain genetic markers in order to customise and optimize therapy.
45
Pharmacogenetics aims to validate and then use some genetic markers in clinical practice in
order to customize and optimize therapy. At first literature search has been done to define the
genetic polymorphisms of possible prognostic/predictive significance. Polymorphisms of
glutathione S-transferase (GST) has been identified, transmembrane transporters (ATPBinding Cassette) that are responsible for the extrusion of the drug from the cell, and
polymorphisms concerning DNA repair genes. 917 patients were enrolled with prostate
cancer from the National Cancer Institute of Aviano (CRO) and 1342 healthy controls of
Friuli Venezia-Giulia region. The analytical methods used for assessing polymorphisms
examined are based on amplification of the portion of the gene of interest by PCR and
subsequent analysis with TaqMan method, which relies on a direct and quantitative
measurement of the amplified DNA using a probe fluorescent, or with the Pyrosequencing
technology, a nanotechnology of recent development, that allows to identify simply and
quickly mutations of punctiform type (SNP). Finally statistical analysis of data was performed
using Fisher's Exact test, has been calculated odds ratios (OR) and corresponding 95%
confidence interval (95%CI) to evaluate the relative risk (OR) of developing prostate cancer.
3° year: Radical radiotherapy, employed alone or in combination with chemotherapy, is one
of the major modalities used in the treatment of cancer. Although several clinicopathologic
indicators, such as prostate-specific antigen level (PSA), Gleason score, pathologic stage, and
surgical margin status, are currently used to predict therapy outcome, there is a need to find
new biomarkers to improve the prediction of disease recurrence. There is evidence that in
addition to patient-related factors such as age and lifestyle, the heterogeneity in response to
radiotherapy in prostate cancer is attributable to a genetic basis. The aim of this study is to
assess the impact of certain genetic variants (polymorphisms) as genes impacting DNA repair,
oxidative stress, phase I and II metabolism and apoptosis, on tumor response (risk to disease
recurrence) to radiotherapy in prostate cancer.
924 prostate cancer patients treated with radiation therapy (adjuvant or radical) in association
or not with surgery and/or hormone therapy were enrolled at the National Cancer Institute of
Aviano (CRO), but only 864 resulted eligible for the evaluation of the risk of biochemical
recurrence. Patients with post-radiotherapy PSA level ≥ 0.2 ng/ml (almost 3 months since the
end of radiotherapy) have been considered to show biochemical PSA recurrence. Genomic
DNA of each patient has been extracted from whole blood. After PCR amplification, genetic
tests have been performed by Pyrosequencing, a nanotechnology that allows to identify
mutations of punctiform type (SNP), TaqMan allelic discrimination, and automated fragment
analysis technologies. Statistical analysis has been done by SAS software (version 9.2) (SAS
Institute Inc., Milan, Italy).
46
1. “NUCLEOTIDE EXCISION REPAIR GENE VARIANTS AND ASSOCIATION
WITH SURVIVAL IN OSTEOSARCOMA PATIENTS TREATED WITH
NEOADJUVANT CHEMOTHERAPY”
P Biason, CM Hattinger, F Innocenti, R Talamini, M Alberghini, K Scotlandi, C
Zanusso, M
Serra and G Toffoli
The Pharmacogenomics Journal (2011), 1-8
2. “A PROSPECTIVE VALIDATION PHARMACOGENOMIC STUDY IN THE
ADJUVANT SETTING OF COLORECTAL CANCER PATIENTS TREATED WITH
THE 5-FLUOROURACIL/LEUCOVORIN/OXALIPLATIN (folfox4) REGIMEN”
E Cecchin, M D’Andrea, S Lonardi, C Zanusso, N Pella, D Errante, E De Mattia, J
Polesel, F Innocenti, G Toffoli
The Pharmacogenomics Journal (2012), 1-7
1. “FARMACOGENETICA DELLA CARDIOTOSSICITÀ DA ANTRACICLINE”
C Zanusso and G Toffoli,
(long abstract for the conference entitled “Complicanze cardiovascolari in oncologia:
ieri ed
oggi. La gestione delle problematiche”, Napoli, March 25-26, 2009)
2. “NANOTECHNOLOGIES AND ONCOLOGY: PHARMACOKINETICS AND
PHARMACOGENOMICS TO OPTIMIZE THE ANTITUMOR THERAPIES”
C Zanusso, E Cecchin, P Biason, E De Mattia, F Sartor and G Toffoli
(Poster presented at the 2nd Phd workshop 10th/11 September 2009, Wittenberg)
(Abstract presented at the “Congresso Nazionale della Società Italiana di
Chemioterapia”, Udine, October 14, 2009)
3. “RADIOGENETICS AND THE OUTCOME OF RADIATION-TREATED
PROSTATE CANCER PATIENTS”
C Zanusso, E Cecchin, R Bortolus, P Biason, E De Mattia, S Boffo, MG Trovò, and G
Toffoli
(Abstract presented at “35° Congresso Nazionale della Società Italiana di
Farmacologia”
Bologna, 14-17 September 2011)
4. “GENETIC POLYMORPHISMS IN THE EARLY ONSET OF OXALIPLATIN
NEUROPATHY AFTER ADJUVANT FOLFOX“
E Cecchin, C Zanusso, M D'Andrea, N Pella, D Errante, S Bonura, M Bari, M Medici,
A
Buonadonna, P Biason, E De Mattia and G Toffoli
47
(Abstract presentato al “35° Congresso Nazionale della Società Italiana di
Farmacologia”
Bologna , 14-17 September 2011)
5. “PHARMACOGENETICS IN OSTEOSARCOMA: THE ROLE OF NUCLEOTIDE
EXCISION REPAIR GENE VARIANTS IN SURVIVAL AFTER NEOADJUVANT
CHEMOTHERAPY”
P Biason, C Zanusso, S Boffo, E De Mattia, E Cecchin, CM Hattinger, M Serra, G
Toffoli
(Abstract presentato al III° Convegno monotematico Società Italiana di Farmacologia
“Farmacogenomica e cancro: dal laboratorio alla clinica”; Grado (GO), 8 October
2011)
6. “FROM BENCH TO BEDSIDE: A GENOTYPE-GUIDED PHASE I STUDY OF
FOLFIRI AND BEVACIZUMAB IN ADVANCED COLON-RECTAL CANCER
PATIENTS”
S Boffo, E Marangon, E Cecchin, E De Mattia, F Innocenti, S Frustaci, A Buonadonna,
GM Miolo, AM Colussi, E Turchet, P Biason, C Zanusso, E Mazzega, P Giusti, G
Toffoli
(Abstract presentato al III° Convegno monotematico Società Italiana di Farmacologia
“Farmacogenomica e cancro: dal laboratorio alla clinica”; Grado (GO), 8 October
2011)
1. “RUOLO DELLA FARMACOGENETICA NEL CARCINOMA PROSTATICO
DELL’ANZIANO”
Convegno GUONE “Il tumore della prostata nell’anziano”, Adria (RO), 23 November
2012
•
•
•
•
•
•
700 hours of study and deepening of the topics covered by books and review-type paper
and / or electronic from January 1, 2009;
700 laboratory hours at the "Experimental and Clinical Pharmacology", CRO Aviano
from January , 2009
and / or electronic from January 1, 2010;
from January 1, 2010
and / or electronic from April 16, 2012;
from April 16, 2012
48
“Congresso annuale delle presentazioni dell’attività scientifica dei dottorandi in
corso”, University of Trieste, January 13-14-15, 2009.
“Seminario sull'uso della VPN e del Portale”, University of Trieste, March 20, 2009.
“Esame Finale 2009”, University of Trieste, April 8, 2009.
“Modeling reactions at the nanoparticle scale” (Prof. Phillip R. Westmoreland Dept of
Chemical Engineering, University of Massachusetts Amherst), University of Trieste, May
8, 2009.
“Chimica fisica delle interfasi in sistemi nanostrutturati” (Dr. Italo Colombo),
University of Trieste, May 15, 2009.
“Incontri informativi per i dottorandi: orientamento all’utilizzo delle risorse e dei
servizi documentali forniti dal Sistema Bibliotecario di Ateneo; panoramicadelle
nuove modalità di comunicazione scientifica: l’archivio istituzionale di Ateneo
OpenstarTs; l’autoarchiviazione delle tesi di dottorato e cenni sull’accesso aperto alla
letteratura di ricerca; procedure di conferimento al sito login miur e all’anagrafe di
Ateneo (Saperi), University of Trieste, June 30, 2009.
“Joint workshop 10th/11 September”, Wittenberg, 2009.
“How to write (and manage) a research project”, (Lecturers: Cecilia Blasetti (Elettra),
Valter Sergo (UNITS), Maurizio Fermeglia (UNITS)), University of Trieste, October 16,
2009.
“Nanotechnology School Annual Meeting 2010”, University of Trieste, January 18, 19
and 20, 2010.
Seminar on 'How to present scientific results' (dr. Hanna Mamzer), University of
Trieste, January 22, 2010.
“Presentation of Nanochallenge and Polymerchallenge by Veneto Nanotech”,
University of Trieste, June 15, 2010.
“Development of On-line Monitoring devices for the extrusion process” (prof. Miguel
Nobrega of the University of Minho in Portugal), University of Trieste, June 15, 2010..
“Use of VPN and UGOV” (prof. Maurizio Fermeglia), University of Trieste, June 15,
2010.
“New Approches in Cancer Therapy” (Prof. Konrad Misiura), University of Trieste,
May 12, 2011.
Seminars (at CRO Aviano):
“Dal Focus 2009: aggiornamenti sul carcinoma mammario”, March 25, 2009.
“Roles of small RNAs in genome defense”, May 7, 2009.
“Seminari in Oncologia al C.R.O. di Aviano”, May 28 and June 4-11-18, 2009.
49
“Matrix revolutions: Perlecan domain V as a novel cancer and stroke therapy” (G.
Bix - Texas A&M College of Medicine- USA), September 14, 2009.
“Invasion and Metastasis in Breast Cancer”, November 23, 2009.
“Bone marrow modelling: new insights regulation of hemopoiesis”, January 13, 2010.
“Young investigators event: la due-giorni dei giovani ricercatori dell’IRCCS CRO”,
March 10 and 11, 2010.
“Targeting tyrosine kinase receptors in solid tumors: the lesson of GISTs”, March 12,
2010.
“Connecting the Dots between Tumor Cell Metabolism and Statins as Anti-Cancer
Agents”, May 3, 2010.
“Recenti progressi in nanomedicina clinica visti da una prospettiva chimico-fisica”,
June 8, 2010.
“Mechanisms controlling the integrity of replicating chromosomes” (dr. Foiani), June
11, 2010.
“Strutturare un protocollo di ricerca” (dr. Foiani), June 11, 2010.
“Multidisciplinarità in UroOncologia”, September 10, 2010.
“The future of technologies (an overview on research technology)”, October 6, 2010.
“Infertilità, gravidanza e tumori”, October 20, 2010.
“Seminari in Oncologia: diagnostica, clinica e ricerca”, October 21, 2010, November
4, 2010, November 11, 2010, November 18, 2010, and November 25, 2010.
“Incontri finalizzati all’esposizione e alla discussione dei progressi ottenuti
nell’ambito dei progetti di ricerca della SOC di FSC” from January 1, 2010 to
December 31, 2010.
“Mitocondri, calcio e morte cellulare per apoptosi e autofagia”, January 27, 2011.
“Introduzione alle nanotecnologie mediche e le loro possibili applicazioni alla
medicina clinica” (prof. G. Scoles), February 2, 2011.
“Introduzione alla Nanobiologia” (prof. G. Scoles) on February 2, 2011.
“Ruolo del complemento nel controllo dello sviluppo tumorale”, February 4, 2011.
“Le associazioni per pazienti oncologici in Europa: il CRO incontra Jan Geissler”,
February 16, 2011.
“Regulation and dysregulation of the p53 network and its role in breast cancer”,
May 31, 2011.
“Targeting cancer stem cells the NGAL: MMP9 connection”, June 8, 2011.
“Nuovi approcci molecolari farmacogenetici, immunogenetica, proteomici e
metabolomici nella ricerca farmacologica”, from January 1, 2011 to June 30, 2011.
“Role of the Hippo pathway effectors YAP and TAZ in mechanotransduction, cell
polarity and cancer stem cells”, May 15, 2012.
50
“Reversal of glucocorticoid resistance by AKT inhibition in T-ALL”, May 24, 2012.
“Aberrant PI3K signalling in lung cancer: from man to mouse and back again”, July
12, 2012.
Conferences
“Congresso Nazionale della Società Italiana di Chemioterapia”, Udine, October 14, 2009.
“Trasferimento Tecnologico. Dinamiche spiegate tramite Case Study, la
Brevettazione in Biomedicina e Biotecnologie”, April 19, 2011.
“DAL FOCUS 2011 SUL CARCINOMA MAMMARIO: assistenza e ricerca clinica
in oncologia”, April 29, 2011.
“Young Investigators Event II: la due-giorni dei giovani ricercatori dell'IRCCS
CRO”, May 3-4, 2011.
“Carcinoma del retto: approccio multidisciplinare, terapie integrate” on May 27,
2011
Courses (at CRO of Aviano):
“Corso di lingua inglese” (Shenker Institute), Pordenone, from January to July 2009.
“RefWorks: creare DataBase Bibliografici personali”, May 7, 2009.
“REFERENCE MANAGER: uno strumento straordinario per ottimizzare la
gestione delle bibliografie”, May 19 and 26, 2009.
“BLSD - Corso teorico pratico di Basic Life Support and Defibrillation”, September
22, 2009.
“L'informazione scientifica in un IRCCS oncologico: formazione all'uso delle risorse
informative rese disponibili dalla Biblioteca del CRO, Bibliosan”, September 29,
2009.
“L'operatore sanitario e la comunicazione in sanità: Power Point come strumento
informatico per presentazioni efficaci”, February 3 and 9, 2010.
“Corso di inglese scientifico livello avanzato”, May 6, 13, 20, 27, June 3, 10, 17, 24,
September 30, October 7, 14, 21, 28, November 4, 11, 2010.
“L’operatore sanitario e la gestione dei dati alfa-numerici: Excel come strumento
per elaborare e visualizzare efficacemente le informazioni”, November 24, December
1 and 7 2010.
•
two hours of class teaching of Pharmacology Oncology of the degree course in
“Biotecnologie Mediche”, University of Trieste, January 15, 2009:
“Gli inibitori della farnesiltransferasi” e “Il proteasoma: ubiquitina e Bortezomib”
51
Dottorandi del 26 ciclo:
DAMIANO CASSESE
Title of the thesis:Design and realization of nanoelectromechanical and plasmonic devices
for Raman spectro-microscopy
Supervisor: Marco Lazzarino
Tutors (if any):
Research Activity
During the first year, the following research activities were carried:
• Design: FEM simulation were performed with COMSOL software to study the
development of a cantilever chip capable of high temperature heating (>400oC) in a
small area (<2 micrometers), to localize the nanowires growth. Following the result
obtained, an optical mask for the chip fabrication was designed with Ledit software.
• Fabrication: the chip fabrication was begun, even though further analysis of structure
compensation in wet etching is needed. Nanowires growth has been achieved on
silicon oxide in PECVD; some tests of growth with MBE are underway.
• Raman spectroscopy: the Raman spectroscopy setup was optimized and the optical
components aligned. Some spectra of various samples of interest (graphene, AlGaAS
nanowires, carbon nanotubes) were taken along with Raman maps aat the micro-scale
using an Andor Technologies spectrograph coupled with JPK AFM movement stage
and a ZEISS inverted microscope.
Objectives for the following year (if applicable)
In the following year we aim to produce the chips with the cantilevers integrated; following
the optimization of the nanowires growth process, the complete devices will be produced.
The chips will be tested, to confirm their TERS potentiality and their behaviour as AFM
cantilevers.
We will also focus on raman spectroscopy and maps of samples, both using micro-Raman and
TERS,
Educational Activity
z Intense course on “Molecular self-assembling and nanostructures”, held by A.
Morgante, L. Casalis, L. Pasquato, 21/06/2011 – 18/07/2011
z Winter collage in Optics: advances in nano-optics and plasmonics (and preparatory
school) at ICTP, to be held from 30th of January till 17th of February
• Massimiliano Di Ventra (Department of Physics University of California, San Diego),
“Fast DNA sequencing: a physicist’s perspective”, seminar at SISSA – Condensed
Matter department
• Workshop on "Circulating Tumor Cells", 7th November 2011, Udine
52
•
•
•
•
Conference Monalisa’s Quidproquo “Nanotechnology Meets Clinical Medicine",
October 6-8, 2011, Aviano/Udine
Summer school of Nanotechnology, Trieste, September 20-23
Konrad Misiura, ‘New Approches in Cancer Therapy’, Trieste
János Kristóf and Erzsébet Horváth, “Synthesis and structure elucidation of kaolinite
organo-complexes”, May 12 2011
53
STEFANIA CORVAGLIA
Titolo della tesi: Modellare substrati su scala nanometrica per la caratterizzazione di singole
cellule
Supervisore: dr. Loredana Casalis, dr. Denis Scaini
Tutori (eventuali):
Attività di ricerca
La ricerca in ambito biomedico dei meccanismi molecolari di patologie come il cancro ha
rivelato come i tessuti malati provenienti da biopsie presentino una distribuzione spaziale
molto eterogenea di popolazioni di cellule tumorali all’interno dello stesso campione che
lasciano supporre differenze sostanziali anche tra singole cellule. Le tecniche attuali riescono
al meglio ad estrapolare dati mediando su campioni di qualche centinaio di cellule e sono
perciò insensibili alle variazioni a livello di singola cellula. Per questo, abbiamo come
obiettivo lo sviluppo di una nuovo metodologia che ci permetta di isolare e caratterizzare
decine di singole cellule dal punto di vista del contenuto proteico (secretoma/proteoma) e di
farne un’analisi statistica. Abbiamo sviluppato un array di micropozzetti delle dimensioni di
una singola cellula, con lo scopo di immobilizzare le cellule in un volume piccolo che
aumenti la concentrazione di proteine rare. Per la detection della proteine stiamo lavorando
allo sviluppo di un nano/microarray di anticorpi specifici, prodotto utilizzando una litografia
AFM-mediata (Nanografting), a chiusura del pozzetto per l’analisi di specifiche proteine,
biomarkers significativi dello stato della cellula. In questo primo anno di dottorato ho lavorato
alla realizzazione degli array di micropozzetti, con particolare attenzione alla scelta del
materiale più adatto alla crescita di cellule, allo studio dell’influenza delle proprietà
meccaniche del materiale sull’adesione e la crescita, all’intrappolamento all’interno del
pozzetto di singole/poche cellule e alla chiusura del pozzetto per l’analisi. Sto testando il
sistema utilizzando un modello cellulare semplice e facilmente reperibile (linea tumorale
HeLa) che faciliti l’applicazione futura a sistemi d’interesse medico.
Pubblicazioni/abstracts in conferenze/congressi (nazionali o internazionali)
- SIBBM Frontiers in molecular Biology, Trieste, Italy- poster abstract_26-28 maggio
2011;
- EBSA 8th European Biophysics congress, Budapest, Ungheria- poster abstract, 23-27
agosto 2011;
- CFN Summer School on Nano-Biology, Karlsruhe, Germany- poster abstract, 7-10
settembre 2011;
- Summer school of Nanotechnology, Trieste, Italy- poster abstract, 20-23 settembre
2011;
- ICTP Conference on Nanotechnology for Biological and Biomedical Applications
(Nano-Bio-Med), Trieste, Italy- poster abstract, 10-14 ottobre 2011
54
Attività formativa
Esami sostenuti, ore di lezione e di laboratorio seguite (data, corso, docente, tipo di corso
di laurea)
- 21 giugno 2011- 15 luglio 2011, corso "Molecular self-assembling and nanostructures"
Lucia Pasquato, Alberto Morgante and Loredana Casalis
Congressi, seminari, corsi avanzati e altre attività didattiche passive
-17-19 gennaio 2011, congressino nanotecnologie, Scuola di dottorato in Nanotecnolgie,
università di Trieste, Trieste, Italy ;
-2 febbraio 2011 workshop biomol @ ELETTRA
Seminari:
-10 febbraio Zehra Sayers, “Structural and functional studies on plant metallothioneins
and heteromeric G-proteins”;
-1 aprile Filippo Lutisani, “Single molecule fluorescence microscopy: new insights”;
-7 aprile Ario de Marco “Application opportunities for single domain antibodies”;
- 3 maggio
-11 maggio Alessandro Laio “Colloquium on condensed matter physics: Exploring the
universe of protein structures beyond the protein data bank”.
-12 maggio Konrad Misiura, “New approaches in cancer therapy”
-13 maggio Giacinto Scoles, “The importance of numbers (with units) and common sense:
the inevitability of solar energy for the resolution of energy need”.
-21 giugno Massimiliano di Ventra, “Fast DNA sequencing: a physicist’s perspective”;
-1 luglio Hlcham Hmamoudi “Self assembled on gold the challenge”
-6 luglio Flavio Maran “Superefficient electron transfer trought 310 –helical peptides”
55
VALENTINA DAL COL
Titolo della tesi: In silico prediction of drug resistance: from cancer targeted therapy to cancer
targeted prevention
Supervisore: Prof.ssa Sabrina Pricl
Tutori (eventuali): Prof. Maurizio Fermeglia
Le recenti scoperte sulla natura dei processi genetici, implicati nelle trasformazioni
neoplastiche, hanno permesso di identificare le lesioni cellulari che promuovono il cancro,
individuando target selettivi per la progettazione di nuovi agenti terapeutici efficaci. Durante
questo primo anno il mio studio si è focalizzato su due diversi sistemi recettoriali: il recettore
tirosin chinasico c-Kit e il recettore σ1. Per il primo sistema si è andati a studiare, attraverso
tecniche di simulazione molecolare, i cambiamenti strutturali che coinvolgono il dominio
JXM (juxtamembrane) del c-Kit in seguito a due differenti mutazioni geniche: Δ559-560 e
V560G. Queste modificazioni portano ad una diversa interazione tra il farmaco d’elezione
(Imatinib) e la tasca di binding recettoriale. Si è visto che la mutazione di delezione (Δ559560) conduce ad uno shifting dell’equilibrio verso la forma attiva della chinasi mentre la
missense (V560G), andando ad alterare la conformazione del dominio JXM, permette una
migliore interazione dell’Imatinib con il recettore nella sua conformazione chiusa.
Si è inoltre sviluppato un modello predittivo 3D, per omologia, del recettore σ1, per il quale è
mancante una struttura cristallizzata. E’ stata utilizzata una procedura multistep: sviluppo e
ottimizzazione della struttura 3D, identificazione del possibile sito di binding, docking di una
serie di ligandi, conseguente calcolo dell’energia libera di binding così da confrontare i
risultati con i valori sperimentali e, alla fine, verifica della capacità predittiva utilizzandolo
per il design di una nuova classe di composti. L’obiettivo finale è renderlo, dopo ulteriori
validazioni, uno strumento per il design di ligandi altamente selettivi e per comprendere
meglio le interazioni che il recettore instaura con altri sistemi biologici.
Pubblicazioni su riviste scientifiche (pubblicate oppure in stampa),
•“2-Difluoromethylene-4-methylenepentanoic acid, a paradoxical probe able to mimic the
signaling role of 2-oxoglutaric acid in Cyanobacteria” Liu X, Laurini E, Dal Col V,
Posocco P, Ziarelli F, Fermeglia M, Zhang CC, Pricl S, Peng L Organic Letters, Jun
3; 13(11):2924-7
•“Homology model and docking-based virtual screening for ligands of the σ1 receptor”
Laurini E, Dal Col V, Mamolo MG, Zampieri D, Posocco P, Fermeglia M, Vio L,
Pricl S ACS Med Chem Lett 2011, 2: 834-839
•“Activate and respond. A molecular rationale for c-kit activation and drug response by
juxtamembrane mutations in GISTs” Dal Col V et al. Mol Cancer Ther 2011
submitted
56
•“Activity vs. toxicity of acridine compounds as anti-BVDV agents: a molecular
modeling study” Dal Col V et al. Antiviral Res 2011 submitted
•Comunicazione a VNPCF Trieste 2011 28-30 marzo “The long and winding road of the
c-Kit JXM domain” Dal Col V et al.
•Comunicazione a VNPCF Trieste 2011 28-30 marzo “Overview on σ1 receptors: a 3D
homology model to solve a part of the enigma” Dal Col V et al.
•Comunicazione a CDDD L’Aquila 2011 21-23 novembre “Nanozapped! DNA, siRNA,
and their dendritic nanovectors: a combined in silico/in vivo/in vitro approach” Dal
Col V et al.
•Comunicazione a CDDD L’Aquila 2011 23-25 novembre “The Sigma-Enigma. A
multistep homology modeling of σ1 receptors” Dal Col V et al.
Comunicazione a Nanotechitaly 23-25 novembre 2011 “Trekking across GISTs: the clinical
journey of KIT, PDGFRA, and the in silico prediction of drug resistance in cancer targeted
therapy” Dal Col V et al.
Attività formativa
di laurea)
60 ore laboratorio MOSE
Scuole
AMBER school 3-6 maggio 2011 Barcellona
Summer school on Nanotechnology 20-23 settembre Trieste
COST Training school 2011 (Dendrimers as composites of advanced drug delivery nano
systems) Atene 3-7 ottobre 2011
Congressi
VNPCF (Vcongresso nuove prospettive in chimica farmaceutica) Trieste 28-30 marzo 2011
CDDD (Computationally driven drug discovery) L’Aquila 21-23 novembre 2011
Seminari
“Brached Peptides as Therapeutics” Prof. Bracci 21 marzo 2011
“Challenging the mistery of marine toxins” Prof. Yasumoto 15 aprile 2011
“New approaches in cancer therapy” Prof. Misiura 12 maggio 2011
“Nanosistemi per il drug e il gene delivery a base di derivati dell’acido aspartico” Prof.
Cavallaro 1 giugno 2011
“Self assembled monolayers the Challenge” Dr. Hicham Hamoudi 1 luglio 2011
“The effect of confinement on enzyme diffusion and reaction inside DNA nanostructures” Dr.
Castronovo 29 luglio 2011
57
Corsi
“Molecular self- assembling and nanostructures”, Prof. Morgante, Casalis, Pasquato
Periodo 21/06-29/07/2011
Altre attività didattiche passive
Congresso dottorandi Scuola di Nanotecnologie 17-19 gennaio 2011
Presentazione delle attività scientifiche dei gruppi di ricerca del dipartimento DI3 25 febbraio
2011
58
ANDREA FRASSETTO
Title of the thesis: Nanostructural analysis of the adhesive interface in dentistry
Supervisor: Prof. Milena Cadenaro
Tutor: Prof. Lorenzo Breschi
Research Activity
The first part of the program was focused on the literature review of the adhesive layer
degradation and nanoleakage development and on laboratory training, including an accurate
analysis at SEM of the interaction of different adhesive systems with the dentin substrate, by
the application of adhesive agents on human dentin surfaces, then stored in appropriate in
vitro conditions to simulate the oral environment for different time intervals in order to assess
nanoleakage expression. Despite acceptable immediate bonding effectiveness of dental
adhesives, the durability of resin bonded interface on dentin created by several bonding
systems remains questionable. All the commercial adhesives tested presented nanoleakage
phenomena after 6 months of aging in artificial saliva. Thus the intrinsic degradation
mechanisms originated from beneath dentine hybrid layer by the slow action of matrix
metalloproteinases (MMPs) was investigated. The release and activation of these endogenous
enzymes during bonding to dentin are thought to be responsible for the in vitro and in vivo
manifestation of thinning and disappearance of collagen fibrils from incompletely infiltrated
hybrid layers in aged, bonded dentine. Collagen degradation generates several collagen
fragments; one of them (ICTP) was quantified by means of commercialized immunoassays.
Moreover the loss of mechanical properties of dentinal collagen was correlated, in particular
the biaxial flexural modulus of elasticity (EBF). Evaluation of both collagen denaturation and
E-modulus could lead to a more clear understanding of the durability of adhesive systems and
nanoleakage development.
• Analysis of new inhibitors of the enzymatic activity of dentin endogenous matrix
metalloproteinases (MMPs)
•
Influence of collagen cross-linkers in the inhibition of MMPs’ activity and subsequent
increase of mechanical properties of the adhesive layer
•
Frassetto A, Navarra CO, Marchesi G, Turco G, Di Lenarda R, Breschi L,
Ferracane JL, Cadenaro M. Contraction stress and degree of conversion of selfadhesive resin cements. Dental Materials, submitted.
•Stress of polymerization of a new self-adhesive vs a conventional dual-cured cement.
XVII National Meeting of the College of Dentistry Teachers, Siena.
59
•
Stress of polymerization and degree of conversion of two self-adhesive vs a
conventional dual-cured cement. 21st European Dental Materials Conference;
Turku (FI).
•
Microtensile bond strength test of four self-etch commercial adhesives. 21st
European Dental Materials Conference; Turku (FI).
•
Influence of chewing simulation on bond strength of cemented composite-disks.
Dent Mater 2011; 27s: e8.
•
Effects of 6-month water storage on micro-tensile bond strength of self-etch
adhesives. Dent Mater 2011; 27s: e10.
•
Influence of chewing simulation on bond strength of cemented ceramicdisks. Dent Mater 2011; 27s: e24.
•
Firenze-Siena 14-16 April 2011; XVII National Meeting of the College of
Dentistry Teachers. Poster Presentation, Stress of polymerization of a new selfadhesive vs a conventional dual-cured cement.
•
San Diego (USA) 16-19 March 2011
•
Augusta (USA) 20-22 March 2011
•
Seili-Turku (FI) 22-26 August 2011
•
Salvador (BR) 13-15 October 2011
•
Course of Material Sciences, CLSOPD II Semester, Prof. L. Breschi
•
Course of Adhesive Dentistry, CLSOPD II Semester, Prof. L. Breschi
•
Trieste 17-20 January 2011; Annual Meeting of the PhD School in
Nanotechnology
•
San Diego (USA) 16-19 March 2011; Annual Meeting of the International
Association of Dental Research
•
Augusta (USA) 21-22 March 2011; Research Stage at Medical College of Georgia
(Prof. D. Pashley)
•
Trieste 8 April 2011; PhD Final Dissertations of School in Nanotechnology
60
•
Firenze-Siena 14-16 April 2011; XVII National Meeting of the College of
Dentistry Teachers
•
Trieste 12 May 2011; Seminar of Prof. K. Misiura: New Approaches in Cancer
Therapy
•
Trieste 1 July 2011; Seminar of Dr. H. Hamoudi: Self assembled monolayers on
gold the challenge
•
Trieste 4-8 July 2011; FVG Summer School/Workshop on Structural
Bioinformatics
•
Trieste 12 July 2011; Seminar of Prof. S.-W. Hla: Nanoscience at Work: Imaging
and Manipulation at Atomic and Molecular Scale
•
Seili (FI) 22-24 August 2011; Dental Materials Summer School
•
Turku (FI) 24-26 August 2011; European Dental Materials Conference
•
Trieste 15 September 2011; Seminar of Dr. G. Forte: Adult Stem Cell and
Thermo-responsive Polymers for Cardiac Muscle Tissue Engineering
•
Trieste 20-23 September 2011; Summer School of the PhD School in
Nanotechnology
•
Salvador (BR) 13-15 October 2011; Annual Meeting of Academy of Dental
Materials
•
Bologna 18-19 November 2011; Annual Meeting of Italian Academy of Prosthetic
Dentistry
•
Milano 25-26 November 2011; Annual Meeting Expo Dental
61
ELISA MINIUSSI
Titolo della tesi: Interaction of metal nanoclusters with graphene and low dimensional
systems
Supervisore: Dr. Alessandro Baraldi
Tutori (eventuali):
Il mio progetto di Dottorato verte sulla produzione e caratterizzazione di nanocluster metallici
depositati su opportune superfici solide, in modo particolare sul grafene cresciuto
epitassialmente su diversi substrati. La mia attività si articola dunque essenzialmente su due
versanti: la crescita epitassiale del grafene e lo sviluppo di una macchina per la produzione e
deposizione di nanocluster selezionati in massa.
Sul primo fronte, un risultato di rilievo è stato recentemente ottenuto con la pubblicazione su
Physical Review Letters di uno studio, iniziato durante la mia tesi specialistica, sul grafene
cresciuto epitassialmente su un nuovo substrato, un cristallo singolo di Re(0001). È noto che
la forza di interazione tra il grafene e il substrato determina la corrugazione del layer di
carbonio, che a sua volta è responsabile delle proprietà di trasporto sia elettronico che termico
di quest’ultimo. Le nostre ricerche hanno evidenziato una diretta correlazione tra la forte
corrugazione del grafene su questo substrato e la sua instabilità ad alte temperature.
È stato inoltre intrapreso lo studio di un nuovo sistema, il grafene cresciuto epitassialmente su
una lega di superficie PtRu, finalizzato a comprendere come variando la concentrazione di Pt
nel primo layer sia possibile modificare selettivamente l’accoppiamento grafene-substrato, e
di riflesso le proprietà morfologiche ed elettroniche del sistema.
In parallelo, sono iniziati i lavori di assemblaggio della sorgente di nanocluster presso il
laboratorio di Fisica delle Superfici (Dipartimento di Fisica dell’Università di Trieste e
Laboratorio TASC (IOM-CNR)). Nell’ambito di questo progetto ho trascorso quattro mesi
con una borsa Erasmus Placement presso il Dipartimento di Chimica Fisica della Technische
Universität di Monaco di Baviera (TUM), dove questa tecnologia è stata sviluppata allo stato
dell’arte nel corso degli ultimi anni. In questo modo ho potuto apprendere i principi di
funzionamento e le modalità operative delle sorgenti e della strumentazione scientifica
utilizzata in combinazione con esse. Ho successivamente sfruttato le conoscenze così
acquisite per portare a termine la fase preliminare di assemblaggio della macchina.
Pubblicazioni su riviste scientifiche (pubblicate oppure in stampa)
E. Miniussi, M. Pozzo, A. Baraldi, E. Vesselli, R. R. Zhan, G. Comelli, T. O. Menteş¸ M. A.
Niño, A. Locatelli, S. Lizzit, and D. Alfè, “Thermal Stability of Corrugated Epitaxial
Graphene Grown on Re(0001)”, Phys. Rev. Lett. 106, 216101 (2011).
Altre pubblicazioni
62
E. Miniussi, M. Pozzo, A. Baraldi, E. Vesselli, R. R. Zhan, G. Comelli, T. O. Menteş¸ M. A.
Niño, A. Locatelli, S. Lizzit, and D. Alfè, “A link between corrugation and thermal stability
of epitaxial graphene”, Elettra Highlights 2010-2011, pagg. 66-67.
Partecipazione a congressi (come relatore)
Presentazione orale del seminario intitolato: “A link between corrugation and thermal
stability of epitaxial graphene”, in occasione dell’assegnazione del premio Fonda-Fasella
2011 conferitomi durante il workshop “Nanoenergetics: theoretical and experimental
approaches” (Trieste, 15-16 Novembre 2011).
Attività formativa
Periodi di permanenza all’estero (data e Sede)
Borsista Erasmus Placement dal 1 aprile 2011 al 31 luglio 2011 presso il Dipartimento di
Chimica Fisica della Technische Universität di Monaco di Baviera (TUM) (tutori presso
l’istituzione ospitante: Prof. U. Heiz e Dr. F. Esch).
di laurea)
“Physikalische Chemie von Nanoteilchen und Oberflächen”
(Chimica Fisica delle
Nanoparticelle e Superfici) http://www.pc.ch.tum.de/index.php?id=193 - Docenti: Dr. F.Esch,
Prof. U. Heiz - Numero di ore: 40
Contenuti: Introduzione alla Chimica Fisica dei cluster: transizione dagli oggetti di
dimensioni atomiche ai solidi di bulk; fabbricazione dei cluster, loro proprietà fondamentali e
caratterizzazione sperimentale (diffrazione, spettroscopia, microscopia); cluster elementari ed
eterogenei, cluster supportati e loro proprietà catalitiche.
Seminar at Elettra: Optical and Dynamical Properties of Hydrogen Bonded Systems
Nadja Doslic, Department of Physical Chemistry, University of Zagreb
10 Febbraio 2011, Seminar Room (Elettra)
Seminar at Elettra: X-Ray absorption spectroscopy and science at extreme conditions:
previous results and new opportunities for the XAFS beamline at Elettra
Giuliana Aquilanti, Sincrotrone Trieste
14 Marzo 2011, Seminar Room (Elettra)
Seminar at Elettra: Images of excited states
Giovanni Piani, Laboratoire Francis Perrin, CEA Saclay, France.
Seminar at Elettra: Electronic Properties of Functionalized Quasi-Free-Standing
Graphene and Monolayer Boron Nitride
Danny Haberer, IFW Dresden, Dresden, Germany
63
Joint Workshop on Energy and Sustainability: Materials and Processes
Chemistry Department, Northwestern University, and Catalysis Research Center, Technische
Universität München
Institute for Advanced Study (IAS) Technische Universität München, Lichtenbergstr. 2a,
85748 Garching, Germany
13-14 Maggio 2011
4. Joint Nanoworkshop of TU/e, DTU and TUM
TUM Institute for Advanced Study (IAS)
TUM Campus Garching
1 Giugno 2011
DFH/UFA Photokat Workshop 2011: Photokatalytische Eigenschaften von
Nanostrukturen / Propriétés photocatalytiques des structures nanométriques
IAS Building, TUM Campus
14-15 Luglio 2011
XI School on Synchrotron Radiation: Fundamentals, Methods and Applications
Duino Castle, Trieste, Italy
5-16 Settembre 2011
First Joint Summer School on Nanotechnology
Università di Trieste, Campus di Piazzale Europa
20-23 Settembre 2011
Seminar at Elettra: Adventures in Catalytic Nanospace: resolving catalytic phenomena at
the atomic scale
Michael Bowker, Wolfson Nanoscience Laboratory and Cardiff Catalysis Institute, School of
Chemistry, Cardiff University, Wales, UK.
2 Novembre 2011, Seminar Room (Elettra)
Workshop on Nanoenergetics: theoretical and experimental approaches
ICTP, Adriatico Guesthouse, Trieste, Italy
15-16 Novembre 2011
Seminar at Elettra: Probing ultrafast symmetry changes with light
Simon Wall, Department of Physical Chemistry, FHI (Berlin)
25 Novembre 2011, Seminar Room (Elettra)
Seminar at Elettra: XAFS at Elettra: recent achievements and future projects
Giuliana Aquilanti, Sincrotrone Trieste
1 Dicembre 2011, Seminar Room (Elettra)
Attività didattica di supporto e attività didattica attiva
Lettura di articoli e review su argomenti attinenti alla mia attività di ricerca, in particolare:
studio sperimentale e teorico delle proprietà del grafene (in generale), crescita del grafene su
64
diversi substrati solidi e sua caratterizzazione, nano cluster metallici e investigazione della
loro morfologia, proprietà elettroniche e reattività.
65
ELISA MITRI
Titolo della tesi: Fabbricazione di dispositivi microfluidici per lo studio della risposta
biologica di cellule vive sottoposte a stimoli chimico-fisici mediante tecniche di
microspettroscopie vibrazionali
Supervisore: Massimo Tormen
Tutori: Gianluca Grenci, Lisa Vaccari
La microspettroscopia infrarossa (MIRS) è considerata tra i più promettenti metodi per lo
screening medico e diagnostico, grazie alle informazioni strutturali e conformazionali,
contenute in uno spettro infrarosso. Lo studio di cellule vive tramite MIRS richiede l'utilizzo
di un set-up microfluidico trasparente all'IR, capace di mantenere le condizioni necessarie alla
vita delle cellule. Negli anni scorsi presso il mio gruppo è stata messa a punto una piattaforma
microfluidica su finestre di CaF2 (materiale trasparente all'IR). Il dispositivo è stato testato
con successo presso la beamline SISSI (Elettra, Trieste). Nel mio primo anno di dottorato ho
lavorato all'ottimizzazione del set-up con l'intento di risolvere i problemi emersi nei
precedenti esperimenti, tra cui:
• Scarsa aderenza del resist durante il processo fabbricativo dovuto alla bassa
energia superficiale del CaF2 (30-50 mJ/m2).
• Effetti sconosciuti del CaF2 sulle linee cellulari (es neuroni)
• Protocollo di chiusura del dispositivo
In riferimento ai punti 1 e 2, abbiamo modificato le proprietà superficiali del CaF2 tramite
sputtering di un sottile strato di Silicio. Questo approccio porta i seguenti vantaggi:
• Possibilità si operare il processo litografico su un substrato Si-like
Assenza di contatto tra cellule e CaF2
•
Per migliorare il protocollo di incollaggio si propone un nuovo metodo che sfrutta la quantità
di solvente residuo nel resist per promuovere l'adesione e la protezione dell'ambiente in cui si
confineranno le cellule. Per dimostrare la validità del nostro approccio abbiamo condotto una
serie di studi, monitorando diverse linee cellulari (MCF-7, HCT116) all'interno del
dispositivo per intervalli di tempo fino a 48 ore.
Pubblicazioni/abstracts in conferenze/congressi
“Optimization of Microfluidic Systems for IRMS real Time Monitoring of Living Cells”
Gianluca Grenci1, Giovanni Birarda1, Elisa Mitri1, Luca Businaro2, Sabrina Pacor3, Lisa
Vaccari4, Massimo Tormen1
1CNR-IOM, Laboratorio TASC – Lilit beam line, Basovizza/Italy, 2 CNR ISTITUTO DI FOTONICA E, ROMA/Italy, 3 Life Science
Dept., Trieste University, Trieste/Italy, 4 Elettra Synchrotron Light Laboratory, SISSI beam line, Basovizza/Italy
“Microfluidic devices for real-time infrared imaging of living cells”
G. Birarda1, G. Grenci2, L. Businaro3, E. Mitri2, M. Tormen2, S. Pacor4 and L. Vaccari1
66
1 Elettra Synchrotron Light Laboratory, ITALY, 2 IOM - CNR, ITALY, 3 Istituto di Fotonica e Nanotecnologie, ITALY, and 4 Trieste
University, ITALY
Attività formativa
Esami sostenuti, ore di lezione e di laboratorio seguite
“Nanotecnologie e nanomicroscopie” (60 h 7,5 CFU laurea specialistica in biotecnologie
mediche) Carlo Dri - Gianluca Grenci
“Biologia Cellulare Animale” (48 h, 5 CFU laurea magistrale in chimica e tecnologie
farmaceutiche) Sabrina Pacor
Seminari
"Synthesis and structure evaluation on kaolinite Organo-Complexes" Prof. Janos Kristof – Dr.
Elisabeth Horvath 03/05/11
“Self assembled monolayers on gold the challenge” Dr. Hicham Hamoudi
“” Dr. Matteo Castronovo
Congressi
Congresso annuale della scuola 17-19 gennaio 2011, Trieste
Conferenze
Wirms 2011 - 6th International Workshop on Infrared Spectroscopy and Microscopy with
Accelerator-Based Sources – 4-9 settembre 2011, Trieste
MNE 2011- 37th International Conference on Micro and Nano Engineerin – 19-23 Settembre
2011 Berlino
67
GIUSEPPINA PALMA
Titolo della tesi: Celle solari dye-sensitized nanostrutturate
Supervisore: Alessandro Fraleoni Morgera
Tutori (eventuali):
Le attività di ricerca del primo anno di dottorato possono essere riassunte in tre punti
fondamentali: 1) Comprensione generale dello stato dell’arte relativo alla fabbricazione ed
ottimizzazione di celle solari a colorante organico; 2) Messa a punto del set up di
caratterizzazione che consiste in un sistema di misura di curve I/V e uno di efficienza
quantica; 3) fabbricazione di dispositivi come da letteratura e studio preliminare di metodi per
lo sviluppo di uno strato semiconduttore altamente poroso da impiegare nelle celle suddette
per aumentarne l’efficienza.
Il punto 1) è stato perseguito mediante uno screening approfondito della letteratura del settore
e la frequenza di una scuola internazionale dedicata al settore fotovoltaico. Il punto 2) è stato
sviluppato dapprima attraverso la frequenza di un corso base di programmazione in Labview,
strumento utilizzato per realizzare i software di guida di un set up di caratterizzazione I/V,
costituito da un simulatore solare accoppiato ad un multimetro, e di un sistema di
determinazione di efficienza quantica. Il punto 3) è stato perseguito cercando di riprodurre le
prestazioni delle DSSC riportate in letteratura, che raggiungono l’11% di efficienza.
Attualmente la realizzazione di dispositivi altrettanto validi non è stata ancora raggiunta, dato
che il lavoro effettivo sui dispositivi è iniziato a partire da Settembre 2011. In particolare, ad
oggi i parametri di fill factor e Voc delle celle realizzate sono in linea con i valori dei
dispositivi stato dell’arte, mentre la corrente estraibile dal circuito è ancora scarsa. Questo
problema verrà affrontato introducendo uno strato compatto di TiO2 tra l’anodo e lo strato
semiconduttore mesoporoso, così da reprimere il forte processo di ricombinazione locale;
inoltre verrà caricata una maggiore quantità di colorante sullo strato mesoporoso attraverso un
processo di multidipping. Inoltre ottimizzazioni dello spessore dello strato nano cristallino e
della struttura generale dei dispositivi (contro-elettrodo, elettrolita, isolamento dall’ambiente
esterno, ecc) dovrebbero portare ad aumentare l’efficienza globale dei dispositivi, attualmente
ferma allo 0.7%.
Studi preliminari sull’utilizzo della metodologia ASB-SANS (Auxiliary Solvent-Based
Sublimation-Aided NanoStructuring) per incrementare l’efficienza delle celle, attraverso
l’aumento di porosità dello strato nanocristallino di TiO2, sono in corso.
• 19-23 Settembre 2011: Nanotech Summer School; Trieste, poster session
• 17-21 Ottobre, 2011: Workshop on New Materials for Renewable Energies, ICTP,
Trieste, poster session
68
Attività formativa
Periodi di permanenza all’estero (data e Sede)
• 11-18 Settebre 2011: Quantsol Summer School; Hirschegg (Austria)
di laurea)
• 7 Aprile- 5 Maggio 2011: Corso base di Labview, G. Cautero, corso di formazione
presso Elettra (16h)
• 21 Giugno- 15 Luglio 2011: Summer course on Molecular self-assembling and
nanostructures (docenti: L. Casalis (8h), A. Morgante (6h), L. Pasquato (6h))
• 17-19 Gennaio 2011: Workshop sulle nanotecnologie (Università di Trieste)
• 03 Maggio 2011 ore 10: Seminario Nanotech: Synthesis and structure elucidation of
kaolinite organo-complexes (János Kristóf and Erzsébet Horváth, University of
Pannonia, Institute of Environmental Engineering, Hungary)
• 03 Maggio 2011 ore 14: Seminario Elettra: The unusual physics of Dirac fermions in
graphene (Alessandra Lanzara, University California, Berkeley)
• 13 Maggio 2011 ore 15: Seminario Elettra: The importance of numbers (with units) and
common sense: the inevitability of solar energy for the resolution of energy needs
(Giacinto Scoles, University of Udine, Faculty of Medicine, Department of Medical
and Biological Sciences, Ospedale di S. Maria della Misericordia in Udine, Italy.
• 27 Maggio 2011: Celle solari polimeriche e dye-sensitized (Alessandro Fraleoni
Morgera, Sincrotrone Trieste)
• 29 Luglio 2011: ‘The Effect of Confinement on Enzymes Diffusion and Reactions
Inside DNA Nanostructures’ (Matteo Castronovo)
• 1 Luglio 2011 ‘Self assembled monolayers on gold the challenge’ Dr. HIcham
Hmamoudi (Université-Paris Sud)
• 19-23 Settembre 2011: Nanotech Summer School; Trieste (Italia)
• 17-21 Ottobre 2011: Workshop on New Materials for Renewable Energies, ICTP,
Trieste, Italy
69
ANDREA RADIVO
Titolo della tesi: Studio sperimentale della fisica di dispositivi fotovoltaici organici
nano strutturati.
Supervisore: Massimo Tormen
Tutori : Simone Dal Zilio, Enrico Sovernigo
In questo primo anno ho concentrato la prima parte del mio lavoro sull’acquisizione delle
conoscenze necessarie al progetto e sulla messa a punto di procedure di preparazione e
caratterizzazione di celle fotovoltaiche organiche. A tal fine, ho quindi prodotto e
caratterizzato celle fotovoltaiche aventi un'interfaccia planare tra strati donore ed accettore,
utilizzando diverse combinazioni di materiali. In una seconda parte del mio lavoro, ho
elaborato un processo per produrre celle fotovoltaiche organiche aventi un'interfaccia nano
strutturata con una struttura interdigitata di materiali donore ed accettore, conformazione che
viene considerata in letteratura come ottimale al fine di incrementarne il rendimento. Ho
scelto, come materiali attivi diverse coppie di piccole molecole organiche e fullereni
ampiamente trattate in letteratura, e come tecnica di nano strutturazione a costo contenuto, il
nanoimprinting. La categoria di materiali in esame offre un’ampia gamma di possibili coppie
di materiali dalle diverse proprietà optoelettroniche, tuttavia pochi di questi sono risultati
adatti al nanoimprinting diretto. Ho quindi scelto un approccio indiretto: nanoimprinting del
substrato PEDOTT-PPS, utilizzato come conduttore/collettore di lacune e facilmente
stampabile, ottenendo una struttura a righe con diversi periodi e larghezze; deposizione di uno
strato conformale di materiale donore per evaporazione; riempimento delle strutture rimanenti
mediate deposizione da soluzione o evaporazione del materiale accettore; evaporazione del
contatto in alluminio per terminare la cella. Ho applicato questa procedura a diversi materiali,
ed i migliori risultati sono stati ottenuti con la coppia Pentacene-PCBM. E’ stato infatti
possibile depositare il pentacene in modo quasi conformale sul substrato nanostrutturato in
PEDOT:PPS e riempire in modo apparentemente completo e con poche porosità le strutture,
con PCBM depositato da soluzione, ottenendo così una struttura interdigitata dei due
materiali.
Obbiettivi da raggiungere per l’anno successivo:
Gli obbiettivi per il prossimo anno di lavoro si possono articolare in:
• Affinare i processi di produzione per avvicinarsi allo stato dell’arte nella qualità e
rendimento delle celle planari;
• Proseguire con lo sviluppo delle celle nano strutturate in pentacene PCBM e trovare altri
materiali per cui il medesimo approccio sia possibile;
• Sviluppare nuovi sistemi di nano strutturazione delle celle fotovoltaiche organiche;
70
• Caratterizzare approfonditamente le celle prodotte per determinare l’effetto della
variazione di processi, strutture e materiali, sul rendimento e le proprietà
optoelettroniche della cella;
•Poster alla Summer school di nanotecnologie. Titolo del poster: Experimental Study of
the physics of nanostructured organic photovoltaic devices.
Attività formativa
Esami sostenuti, ore di lezione e di laboratorio seguite:
Formazione in laboratorio all’utilizzo di strumenti di caratterizzazione e fabbricazione celle
fotovoltaiche presso laboratorio TASC e Elettra. In particolare:
•Caratterizzazione: formazione per l’utilizzo autonomo di AFM, SEM, Simulatore solare,
diffrattometria RX profilometria e fotoluminescenza.
•Fabbricazione: formazione su macchinari e nozioni necessarie a deposizione film per
spin coating, evaporazione termica a vuoto, sputtering, deposizione elettrochimica;
formazione utilizzo pressa per il nanoimpriting, tecniche per il patterning di substrati
e celle (attacco chimico e fotolitografia).
• Trattamenti substrati e celle: formazione per l’utilizzo di forno ossidativo, RIEE,
tecniche di pulizia e funzionalizzazione substrati.
Attività organizzate dalla scuola di dottorato:
•Summer school of nanotechnology dal 20 al 23 settembre.
•Seminario: Matteo Castronovo. The Effect of Confinement on Enzymes Diffusion and
Reactions Inside DNA Nanostructures. July 18 2011
•Seminario: János Kristóf and Erzsébet Horváth; synthesis and STRUCTURE elucidation
of kaolinite organo-complexes
•Seminar: Dr. Hicham Hmamoudi ‘Self assembled monolayers on gold the challenge’
July 1, 2011
Attività al di fuori della scuola di dottorato:
•Workshop on New Materials for Renewable Energy 17 - 21 ottobre 2011 (ICTP,
Miramare, Trieste, Italy). (36h di seminari, poster session, con certificato di
partecipazione).
•Presentazione: Michael Felsmann, da Gatan, presentazione sulle tecniche EELS, TEM e
SAM ed I recenti avanzamenti in queste tecnologie. (1h)
•Seminario: Serdar Sariciftci on organic photovoltaic at ICTP 13 Aprile (1h)
71
Studio di articoli scientifici e rewiews riguardanti le celle fotovoltaiche organiche o
l’elettronica organica in generale.
72
MICHELE ROMEO
Titolo della tesi: “Sviluppo e applicazione di metodologie DFT e TDDFT per la
descrizione di osservabili spettroscopici in sistemi condensati”
Supervisore: Prof. GIOVANNA FRONZONI (DSCF)
Tutori (eventuali):
L'adsorbimento di molecole organiche su superfici semiconduttrici ha attratto un'attenzione
crescente per la sua importanza nelle tecnologie emergenti.
La spettroscopia NEXAFS viene largamente utilizzata per caratterizzare strutture adsorbite su
superfici dal momento che permette di investigare i vari modi di adsorbimento così come
l'estensione dell'interazione adsorbato-substrato. Computazioni quantistico-chimiche sono
importanti per acquisire la maggior parte di informazione dagli esperimenti che sono spesso
di difficile interpretazione e razionalizzazione. L'obiettivo principale del progetto è lo
sviluppo di uno schema computazionale utile per la simulazione di spettri NEXAFS di
molecole adsorbite su superfici nel contesto del design di modelli molecola/superficie ed in
quello del calcolo ed interpretazione dei risultati spettroscopici. Durante il primo anno di
dottorato è stata condotta una simulazione di spettri NEXAFS di etilene adsorbita su una
superficie regolare di Silicio, nella fattispecie Si(100), considerando diverse geometrie di
adsorbimento. Un primo step ha riguardato l'implementazione di un codice di interfaccia con
ADF (Amsterdam Density Functional code) per il calcolo si spettri NEXAFS risolti
angolarmente per date direzioni di polarizzazione della luce incidente secondo l'espressione
canonica di calcolo. In un secondo step sono stati ottimizzati modelli di superfici adsorbenti
con e senza le molecole di etilene per mezzo di computazioni DFT periodiche su opportuni
geometrizzazioni di slab. Sono state considerate entrambe le geometrie di adsorbimento ontop e bridge. Dalle strutture risultanti dal calcolo periodico sono stati estratti razionalmente
cluster finiti utili alla successiva computazione degli spettri NEXAFS dell'etilene a livello
C1s. Il confronto fra gli spettri risolti angolarmente calcolati e quelli sperimentali è risultato
soddisfacente e mette in luce l'evidente potenziale della tecnica computazionale nello studio
delle configurazioni di adsorbimento di molecole su superfici.
“C K-edge NEXAFS Spectra of Model Systems for C2H4 on Si(100): a DFT Simulation”,
contenuti riportati negli “Atti del XXIV Congresso Nazionale della Società Chimica
Italiana” per la Divisione Computazionale
Attività formativa
di laurea)
z Marzo - Giugno 2011, Chimica Quantistica, Prof. Mauro Stener, corso specialistico
73
Marzo - Giugno, 2011, Applicazioni Chimiche della Simmetria Molecolare, Prof.
Piero Decleva, corso specialistico
• Serie di seminari su Molecular Self-assembling and Nanostructures, 21 Giugno – 15
Luglio, 2011, Trieste
• Scuola Estiva del FVG su Bioinformatica Strutturale, 4-6 Luglio 2011, SISSA, Trieste
• Scuola Estiva su Tecniche di Simulazione Atomistiche, 11- 29 Luglio 2011, SISSA,
Trieste, (affiliazione CECAM-SISSA)
• Scuola Estiva di Nanotecnologia 2011, 20-23 Settembre 2011, Scuola di Dottorato in
Nanotecnologie, Trieste
• Workshop su Nanoenergetica, 15-16 Novembre, 2011, ICTP, Trieste
• Analisi Matematica, Fisica ed attività di esercitazione per studenti del 1o anno dei
corsi fondamentali di Fisica
• Attività di esercitazione intensiva per studenti del 1o anno dei corsi di STAN e
z
Scienze Geologiche
74
SAJID HUSSAIN
Title of the thesis: Synthesis of Ordered Semiconductor Nanostructures by Directed SelfAssembly for Photonic Applications
Supervisor: GIORGIO BIASIOL
Research Activity
Silicon mold with 45 - 50nm diameter pillars, with a height of 90nm & period of 300nm have
been fabricated using nanoimprint lithography. These molds are used in a second nanoimprint
lithography step to pattern the GaAs substrates used for QDs growth. Different nanoimprint &
optical resist have been tried, but good surface quality (i.e. RMS surface roughness ≥ 0.2nm)
of GaAs substrate after the removal of the resist have been achieved using mr-I 7010E. After
hot plate pressing of the mold and resist coated GaAs wafer, there is a very thin residual layer
of resist inside the holes underneath of the pillars. This residual layer is etched using oxygen
plasma in Inductively Coupled Plasma (ICP) system. These GaAs patterned substrates are
chemically etched to get 50-60nm dia holes with a 5 – 10nm depth. Different solutions (i.e.
HCl:H2O2:H2O & NH4OH:H2O2:H2O) with different dilution have been tried to fabricate
these nanopores. Best results have been achieved with HCl:H2O2:H2O solution with a ratio of
1:1:9. Substrate is cleaned using wet chemistry & oxygen plasma in Reactive Ion Etching
(RIE) system. Then oxide layer from the patterned GaAs substrate is thinned chemically
before its introduction into the Molecular Beam Epitaxy (MBE) System. Silicon molds and
patterned GaAs substrates have been analysed using Scanning Electron Microscopy &
Atomic Force Microscopy at different stages of the process. Then patterned GaAs substrate is
loaded in MBE system for QDs growth. Since thermal removal of the oxide before growth
would lead to a disappearance of the holes and to a roughening of the surface. We are testing
a method to desorb chemically the oxide through exposure to atomic Ga beams, followed by
annealing in As4 and deposition of a thin (<10nm) GaAs buffer before deposition of InAs
QDs. Understanding & optimization of the growth kinetics and parameters of QDs growth
using MBE System is in process.
Kinetics and parameters of QDs growth using Molecular Beam Epitaxy System will be
studied. All parameters of QDs growth (i.e. Oxide layer removal using Ga flux, annealing,
buffer layer thickness, InAs QDs growth) will be optimized using Molecular Beam Epitaxy
System.
Currently, nanopores on GaAs substrate are being fabricated using wet etching. As an
alternative to reduce hole size, dry etching will be used to fabricate these nanopores using
RIE/ICP system.
Optical characterization of the QDs samples will be done using photoluminescence & microphotoluminescence spectroscopy.
75
Seminar of Dr. HIcham Hmamoudi from Université-Paris Sud on ‘Self assembled monolayers
on gold the challenge’ at University of Trieste on July 1, 2011.
Seminar of Matteo Castronovo on ‘The Effect of Confinement on Enzymes Diffusion and
Reactions Inside DNA on July 18 2011.
The regional Summer school on Nanotechnology 2011 at University of Trieste on 20 -23
September 2011
Literature study/survey for material selection for QDs fabrication.
Research papers/thesis/articles have been studied to carry out the research work.
76
FRANCESCA SANTESE
Titolo della tesi: Modellistica molecolare per materiali e rivestimenti multifunzionali
nanostrutturati
Supervisore: Prof. Maurizio Fermeglia
Tutori (eventuali): Prof.ssa Sabrina Pricl
Durante il mio primo anno di dottorato ho concentrato la mia attività di ricerca su due progetti
principali. Il primo è un progetto europeo, Multhybrids, il cui obiettivo primario era lo
sviluppo di una tecnologia innovativa per la preparazione di componenti con nanomateriali
multifunzionali; il secondo è un progetto nazionale, Nanostrata, che ha come scopo lo
sviluppo di nuovi rivestimenti nanostruttuati per diverse applicazioni industriali. In
Multhybrids abbiamo caratterizzato 13 sistemi con nanofillers di diversa natura e forma, puri
o modificati con diversi compatibilizzanti, in diverse matrici polimeriche, utilizzando una
procedura di modellistica molecolare multiscala sviluppata dal nostro gruppo di ricerca. I
risultati ottenuti sono incoraggianti vista l’ottima concordanza dei valori delle proprietà
termofisiche calcolate con i corrispetivi dati sperimentali. Inoltre, si è visto che nei sistemi
esfoliati le caratteristiche chimico/fisiche dei modificatori non influiscono sulle proprietà
macroscopiche, mentre le dimensioni e la forma dei nanofillers hanno un grande impatto sulle
proprietà del materiale finale. In Nanostrata abbiamo svolto uno studio sulla bagnabilità di
superfici polimeriche con liquidi di diversa natura (acqua, olio, miscela di acqua e sapone) per
sviluppare una metodologia che consenta di predire alcune importanti proprietà
dell’interfaccia, quali l’angolo di contatto, la tensione superficiale e il lavoro di adesione. La
concordanza dei risultati ottenuti con i valori sperimentali disponibili in letteratura ha
permesso di convalidare la procedura sviluppata. Si potrà quindi procedere con la
progettazione di nuovi rivestimenti andando a modificare le matrici polimeriche con
l’aggiunta di nanoparticelle di diversa natura.
“Size and shape matter! A multiscale molecular simulation approach to polymer
nanocomposites” R. Toth, F. Santese, S. P. Pereira, D. R. Nieto, S. Pricl, M. Fermeglia and P.
Posocco, Journal of Material Chemistry, 2011 sottomesso
“Contact angles of water and oil on polymer surfaces by MD simulations” F. Santese, D. R.
Nieto, P. Posocco, R. Toth, S. Pricl, M. Fermeglia, Chemical Communications, 2011,
sottomesso
“Water, oil, and surfactant solution on polymer surfaces: converging simulation methods for
contact angle determination” F. Santese, D. R. Nieto, P. Posocco, R. Toth, S. Pricl, M.
Fermeglia, Journal of Physical Chemistry C, 2011 sottomesso
77
AIChE Annual Meeting 2011, comunicazione: “The Factory of the Future: Integrating
Multiscale Modeling and Experiments to Produce New, Better Nanocomposite Materials” M.
Fermeglia, P. Posocco, R. Toth, D. R. Nieto, F. Santese and S. Pricl, 24 ottobre 2011
Minneapolis
Eurofillers 2011 comunicazione: “Chemistry and shape effects in polymer based
nanocomposites: a multiscale modeling study.” M.Fermeglia, P. Posocco, R. Toth, D.
Romero, F. Santese, S. Pricl 21-22 agosto 2011 Dresden, Germany
NanotechItaly, comunicazione: “Nanoparticles at large. A multiscale molecular modeling
protocol to predict/explain structure-property relationships in nanocomposite systems” P.
Posocco, F. Santese, D. R. Nieto, E. Laurini, M. Fermeglia, J. W. Handgraaf, H. G.E.M.
Fraaije, M. Lisal, S. Pricl, 23-25 novembre 2011, Venezia
NanotechItaly 2011, comunicazione: “Interfacial wettability of polymeric surfaces by oil,
water and surfactant/water nanodroplets. A molecular dynamic study” F. Santese, D. R.
Nieto P. Posocco, R. Toth, M. Fermeglia, S. Pricl, 23-25 novembre 2011, Venezia
Attività formativa
di laurea)
60 ore laboratorio MOSE
Scuole
AMBER school 3-6 maggio 2011, Barcellona
Summer school on Nanotechnology 20-23 settembre, Trieste
Congressi
VNPCF (V congresso nazionale di chimica farmaceutica) Poster: “The long and winding
road of the c-Kit juxtamembrane domain”, 28-30 marzo 2011, Trieste
Seminari
“Brached Peptides as Therapeutics” Prof. Bracci 21 marzo 2011
“Seminario GPU@UniTS - Processori Grafici & Calcolo Intensivo” 18 febbraio 2011
“New approaches in cancer therapy” Prof. Misiura 12 maggio 2011
“Nanosistemi per il drug e il gene delivery a base di derivati dell’acido aspartico” Prof.
Cavallaro 1 giugno 2011
“Self assembled monolayers the challenge” Dr. Hicham Hamoudi 1 luglio 2011
“The effect of confinement on enzyme diffusion and reaction inside DNA nanostructures” Dr.
Castronovo 29 luglio 2011
78
Corsi
“Molecular self- assembling and nanostructures”, Prof. Morgante, Casalis, Pasquato Periodo
21 giugno-29 luglio 2011
Altre attività didattiche passive
Congresso dottorandi Scuola di Nanotecnologie 17-19 gennaio 2011
Presentazione delle attività scientifiche dei gruppi di ricerca del dipartimento DI3, 25 febbraio
2011
79
LETIZIA TAVAGNACCO
Titolo della tesi: Ruolo dell'acqua nel riconoscimento molecolare di bionanostrutture in
sistemi alimentari.
Supervisore: Prof. Attilio Cesàro
Tutori (eventuali): Prof. John W. Brady
Il tema di questo progetto di dottorato è rivolto alla identificazione di possibili parametri
molecolari che controllano la sicurezza e la qualità degli alimenti. Particolare attenzione è
rivolta verso l'acqua negli alimenti ed il suo ruolo fondamentale all'interfaccia di superfici
biomolecolari. L'obbiettivo generale è ottenere informazioni sulla strutturazione dell'acqua in
alimenti tradizionali attraverso l'utilizzo di una varietà di tecniche sperimentali a diversa
risoluzione.
In questo primo anno di dottorato, si è considerato il sistema caffè, e in particolare la caffeina
è stata identificata come molecola modello di studio in quanto costituita da una superficie
idrofobica ma solubile in acqua grazie alla presenza di gruppi funzionali capaci di formare
legami a idrogeno. Attraverso simulazioni di dinamica molecolare è stato possibile
caratterizzare il dettaglio molecolare della strutturazione dell’acqua attorno alla caffeina e
l’associazione del soluto in soluzione. In particolare la caffeina si è rivelata un buon sistema
perché ha reso possibile il confronto tra risultati computazionali e misure sperimentali e
recenti teorie sull’idratazione di soluti idrofobici. Sono inoltre stati condotti esperimenti
SAXS su soluzioni acquose di caffeina e preliminari misure AFM.
Attraverso simulazioni di dinamica molecolare è stata inoltre valutata l’associazione di
biomolecole in soluzione acquosa e in particolare l’interazione della caffeina con zuccheri
quali glucosio e saccarosio.
Obbiettivi da raggiungere per l’anno successivo (se applicabile)
I principali obbiettivi per il seguente anno di dottorato riguardano innanzitutto il
completamento degli esperimenti di SAXS e AFM. Successivamente un sistema modello di
estesa superficie idrofobica sarà caratterizzato attraverso l’uso di diverse tecniche
sperimentali e computazionali.
L. Tavagnacco, P. E. Mason, U. Schnupf, F. Pitici, L. Zhong, M. E. Himmel, M.
Crowley, A. Cesàro, J. W. Brady, “Sugar-binding sites on the surface of the
carbohydrate-binding module of CBH I from Trichoderma reesei” Carbohydr. Res., 2011,
345(6), 839-846.
L. Tavagnacco, U. Schnupf, P. E. Mason, M-L. Saboungi, A. Cesàro, J. W. Brady,
“Molecular dynamics simulation studies of caffeine aggregation in aqueous solution”, J.
Phys. Chem. B, 2011, 115(37), 10957-10966.
80
J. W. Brady, L. Tavagnacco, L. Ehrlich, M. Chen, U. Schnupf, M. E. Himmel, M-L.
Saboungi, A. Ceasàro, “Weakly-hydrated surfaces and the binding interactions of small
biological solutes”
Eur. BioPhys. J. DOI 10.1007/s00249-011-0776-2 (in stampa).
L. Tavagnacco, A. Cesàro, J. W. Brady, “Thermodynamics of aqueous caffeine selfassociation: a revisitation”, “10th Mediterranean Conference on Calorimetry and Thermal
Analysis - MEDICTA 2011”, 24 - 27 Luglio 2011, Porto, Portogallo.
Partecipazione a congressi (come relatore)
L. Tavagnacco, A. Cesàro, U. Schnupf, P. E. Mason, J. W. Brady, “Hydration and
association of biomolecules in aqueous solution”, Studium Conference “Cosmetics and
Pharmaceutics: New Trends in Biophysical Approaches”, 14-15 Febbraio 2011, CNRSOrleans, Francia (presentazione orale).
L. Tavagnacco, A. Cesàro, J. W. Brady, “Thermodynamics of aqueous caffeine selfassociation: a revisitation”, “10th Mediterranean Conference on Calorimetry and Thermal
Analysis MEDICTA 2011”, 24 - 27 Luglio 2011, Porto, Portogallo (presentazione orale).
L. Tavagnacco, M. Borgogna, J. W. Brady. A. Cesàro, “ How do water molecules probe
and control bionanostructures and food functionalities”, Nanotechnology Summer School
2011, 20 – 23 settembre 2011, Trieste (presentazione poster).
L. Tavagnacco, M. Borgogna, J. W. Brady. A. Cesàro, “ How do water molecules probe
and control bionanostructures: caffeine and sugars”, Studium Conference “Water in
biological systems”, 5 – 6 Dicembre 2011, CNRS-Orleans, Francia (presentazione poster).
Attività formativa
Esami sostenuti, ore di lezione e di laboratorio seguite (data, corso, docente, tipo di
corso di laurea)
- “Meccanica Statistica”, Prof. C. Micheletti, 8 novembre 2011 – 28 febbraio 2012, Phd
course, Sissa, Trieste.
- Laboratorio SAXS, Dr. H. Amenitsch, Elettra, Trieste, marzo – giugno 2011.
- Laboratorio AFM, Dr. D. Scaini, Nanolab, Elettra, Trieste, settembre – novembre 2011.
Nanotechnology School Congress, 17-19 gennaio 2011, Trieste.
Seminario "Synthesis and structure elucidation of kaolinite organo-complexes”, János
Kristóf e Erzsébet Horváth, Trieste.
Seminario “The Effect of Confinement on Enzymes Diffusion and Reactions Inside DNA
Nanostructures”, Matteo Castronovo, Trieste.
81
Corso intensivo "Molecular self-assembling and nanostructures", organizzato dalla Scuola
di Nanotecnologie, Trieste.
Giornate didattiche 2011 SISN, “Introduzione alle tecniche neutroniche per lo studio
microscopico della materia, con applicazioni alla Fisica, Chimica, Biologia e Geologia”,
25 giugno – 5 luglio 2011, Valle Aurina (Bz) – Grenoble (Francia).
XI Scuola di Radiazione di sincrotrone , “Radiazione di Sincrotrone: fondamenti, metodi e
applicazioni”, 5 – 16 settembre 2011, Castello di Duino, Trieste.
Nanotechnology Summer School 2011, 20 – 23 settembre 2011, Trieste.
Laboratorio di Chimica delle Macromolecole II, corso di laurea magistrale in Chimica,
Prof. A. Cesàro (attività didattica di supporto).
82
SHITAL VAIDYA
Title of the thesis: Study of magnetic properties in low dimentionality systems using
synchrotron radiation
Supervisor: Dr. Roberto Gotter
Tutors (if any):
Research Activity
Measurement of magnetic properties of NiO/Ag antiferomagnetic films by means of x-ray
magnetic linear dichroism (XMLD) at the ALOISA beam line of ELETTRA. For the
estimation of the dichroism, an accurate set of parameters have been chosen for fitting the Ni
L2 edge and then calculating areas under the peaks corresponding to Ni L2 edge. Neel (TN)
transition temperature has identified by fitting the dichroism as a function of the temperature
by means of power law exponent function for two dimensional systems.
Angle resolved Auger photoelectron coincidence spectroscopy (AR-APECS), which is able to
disentangle high spin and low spin contribution to the Auger intensity, has been performed
below and above TN in order to deepen the sensitivity of the techniques to the local magnetic
order. The data analysis for data as acquired by the new experimental set up is under
development. In the mean time a subsequent experiment on a prototypical ferromagnetic
system, Ni/Cu(001), is going to be prepared with preliminary characterizations for in-situ
sample synthesis.
Theoretical study has been done by collaborating groups. On one side AR-APECS for
ferromagnetic systems has been explained by convoluting DFT ab-initio calculated DOS of
majority and minority sub-bands and on another side AR-APECS has been explained by
means of atomic-like high-spin and low-spin multiplet terms, for antiferromagnetic systems.
On progress experiments aim to join the two findings in a single modelisation, by exploiting
the Cini-Sawatzky theory, describing the trend of the Auger spectra from band-like to atomiclike behaviors depending upon the degree of electron correlation of the system (material,
dimensionality, size) and to understand why dichroism in AR-APECS, which is related to
local electronic structure, disappears above the magnetic transition temperature (indeed it is
believed that only long range magnetic order disappear above them).
Date
Course
Professor
24/06/2011
Molecular self-assembling Loredana
and nanostructures
Casalis
Corso per dottorato
27/06/2011
Molecular self- assembling Alberto
and nanostructures
Morgante
Corso per dottorato
83
Type of course
28/06/2011
Molecular self-assembling Alberto
and nanostructures
Morgante
Corso per dottorato
5/07/2011
and nanostructures
Casalis
Corso per dottorato
7/07/2011
and nanostructures
Casalis
Corso per dottorato
11/07/2011
Molecular self-assembling Lucia
and nanostructures
Pasquato
Corso per dottorato
Molecular self-assembling Lucia
and nanostructures
Pasquato
Corso per dottorato
13/07/2011
1) XI School on synchrotron radiation: Fundamentals, methods and applications. 5-16
September 2011.
2) Summer school of nanotechnology. 20-23 September 2011.
84
VIRGILIO FRANCESCA
Titolo della tesi: “Sviluppo di sensori elettrochimici mediante processi nanotecnologici
per impieghi diagnostici biologici e medici”
Supervisore: Massimo Tormena, Paolo Ugob
Tutori (eventuali): Mauro Prasciolua
a
b
CNR-IOM, laboratorio TASC, Basovizza SS14 km 163.5, 34149 Trieste;
Dipartimento di Scienze Molecolari e Nanosistemi, Unversità Ca’ Foscari di Venezia, Santa
Marta 2137, 30123 Venezia.
La fabbricazione degli array di nanoelettrodi è stata eseguita attraverso la deposizione di un
film sottile di polimero (strato isolante) su uno strato di materiale conduttore. Il film
polimerico è stato successivamente “patternato”, attraverso litografia elettronica, in modo tale
da esporre delle aree, di forma e geometria definita, dello strato conduttore sottostante. Nella
prima parte dell’attività di ricerca sono stati quindi valutati i materiali da utilizzare per questo
processo di fabbricazione ed è stato svolto il training per l’utilizzo dell’electron beam
litography (EBL), presso il laboratorio di microfabbricazione del TASC1. Quindi sono stati
ottimizzati i parametri litografici per i materiali selezionati e sono stati ottenuti i primi array
di nanoelettrodi. Nell’ultima parte dell’anno gli array ottenuti sono stati caratterizzati
elettrochimicamente presso il Laboratorio di sensori per elettroanalisi (LSE)2 attraverso
misure di voltammetria ciclica e testati con il label luminoforo Ru(bpy)32+ per valutare
l’applicabilità di una strategia di rivelabilità di tipo elettrochemiluminescente. Gli studi
preliminari hanno dimostrato che l’ampia finestra di potenziale di questi sistemi può
consentire di utilizzare il Ru(bpy)32+ come strategia di rilevamento. La bassa corrente di fondo
del BDD (Boron Doped Diamond, materiale conduttore selezionato per la fabbricazione)
aggiunto alle proprietà dei NEA e l’elettrocatalisi del sistema Ru(bpy)32+/ammina indicano
che questo sistema può essere applicato per lo sviluppo di sensori ad alta sensibilità. Nella
fase successiva si prevede di funzionalizzare opportunamente la superficie del polimero/resist
(policarbonato) con molecole biologiche per verificare l’applicabilità nel campo di
riconoscimento molecolare.
Altre pubblicazioni
• Presentazione Poster durante la Summer School on Nanotechnology
1
Laboratorio Nazionale CNR-IOM, S.S. 14 Km 163.5 Basovizza - 34012 Trieste.
2
Dipartimento di Scienze Molecolari e Nanosistemi, Unversità Ca’ Foscari di Venezia, Santa Marta 2137, 30123 Venezia.
85
Attività formativa
di laurea).
Lezioni:
20h Processi elettrodici, primo semestre 05/05/2011–25/05/2011,
Prof. C. Tavagnacco, Corso di Laurea Specialistica in Chimica.
Trainings:
¾ Scanning Electron Microscopy, dott. Regina Ciancio, Researcher
CNR-IOM, TASC Laboratory;
¾ Electron Beam Lithography, dott. Mauro Prasciolu, Researcher
CNR-IOM, TASC Laboratory.
Libri:
• Yamazaki K. “Electron Beam Direct writing” Nanofabrication
Fundamentals and applications Ed. Ampere A. Tseng, Ch.10, 341376.
• Moretto L.M. et al. “Templated Ensembles of Nanoelectrodes”
Handbook of Electrochemical Nanotechnology vol.1, Ed. Yuehe Lin
and Hari Singh Nalwa, Ch.4, 87-105.
Reviews:
o Chen Y., Pepìn A. “Nanofabrication: Conventional and
nonconventional methods” Electrophoresis 22, 2001, 187-207;
o Arrigan D.W.M. “Nanoelectrodes, nanoelectrode arrays and their
applications” Analyst 129, 2004, 1157-1165;
o Richter M.M. “Electrochemiluminescence (ECL)” Chem. Rev. 104,
2004, 3003-3036.
• “Syntesis and structure elucidation of kaolinite organo-complexes” J. Kistof and E.
Horvath; “Virtual private network” M. Fermeglia; 03.05.2001
• “Novel Studies in Photoelectrochemistry” H.B. Yildiz, 24.05.2011
• “Self assembled Monolayer the challenge” H. Hamoudi, 01.07.2011
• FVG Summer School on structural bioinformatics 04-06.07.2011
• “The effect of confinement on enzyme diffusion and reaction inside DNA
nanostructures” M. Castronovo, 29.07.2011
• XI School on Synchrotron Radiation: Fundamentals, Methods and Applications.
05-16.09.2011
• Summer School on Nanotechnology. 20-23.09.2011
86
Dottorandi del 27 ciclo
ABDOLLAHZADEH Iman, ANGELONI Valeria, BORIN Daniele, CAPOLLA Sara,
CECCHINI Paolo, COCEANO Giovanna, DIOLOSA‘ Marina, ELISEI Elena, FORNASARO
Stefano, GANBOLD Tamiraa, IONESCU Andrei Cristian, LOVAT Giacomo, MARSON
Domenico, NKOUA Ngavouka Maryse Dadina, OTTAVIANI Giulia, PANIGHEL Mirco,
PIANTANIDA Luca, PITTIA Paola, SALGADO Bernardes Pereira Simâo Pedro, SCOTTI
Nicola, STOKELJ Tina, STOPAR Alex, TARUSHA Lorena, VEGA Marienette,
VENTURELLI Leonardo, WANG Lianqui, ZANNIER Valentina.
87
Progetti Dottorandi del 28 ciclo:
Sono inclusi in questa parte del documento i progetti scientifici dei seguenti dottorandi del 28
cilco:
AMNA ABDALLA MOHAMMED KHALID
Email: [email protected]
Title of the thesis: Atomic force microscopi investigation of the structural properties of
DNA replication origins in tumor cells
Laboratory: NanoInnovation Laboratory- Sincrotrone Trieste
Supervisor: Dr. Loredana Casalis & Dr. Silvia Onesti
Tutors (if any):
Research Activity foreseen
State of the art and motivation
Basic cellular processes as DNA replication are crucial to cell life. Understanding at the
molecular level the mechanisms that govern DNA replication in proliferating cells is
fundamental to understand disease connected to genomic instabilities, as genetic disease and
cancer.
The process of replication, in human cells begins specific sites on the gnome called
“replication origins”, whose activity is finely rregulated during the cellular cycle, and
involves several proteins which recognize the origins and recuit other protein or protein
complexes responsible for DNA unwinding and replication. From preliminary studies, it
seems evident that DNA topology plays a fundamental role in the replication process, and that
protein factors should be responsible for the correct assembling of replication complexes.
Our idea is to use Atomic Force Microscopy (AFM) to study topology features on genomic
DNA associable to replication origins, in the presence different protein factors. Also, we plan
to highlight the unwinding mechanism of MCM (mini-chromosome maintenance) helicase
complex. AFM can be used to detect the morphology, the stiffness and the frictional forces of
any kind of material, in almost any kind of environment.
88
During my ICTP Diploma thesis project, I started working with AFM to studythe interaction
of DNA with MCM protein complexes, which are supposed to form a ring around one strand
and unzip the DNA by moving along, using energy from ATP hydrolysis.
Objectives for the three years
The main goal of the project is to identify topological properties of DNA replication origins,
to understand the mechanism of DNA replication with hope to learn how to control the
replication process in tumor cells. Moreover, since MCM is expressed only in proliferating
cells it could be considered as a good candidate for novel cancer biomarkers, and could be
tested in my lab (NanoInnovation lab) using the nanoarry and the microfluidic immunosensor
technologies developed there.
Objectives for the first year
¾To learn different biochemical and single molecule techniques to be used during the
research project.
¾To concentrate our work on the optimization of the mica surface properties in order to
bind DNA molecules as well the DNA-protein complexes, which is a necessary step
for AFM imaging, without disturbing DNA free conformation, to study DNA
topology.
¾Final step for the first year, will be to perform AFM images in air and liquid with high
resolution.
Research project
The structural properties of the DNA origin can be understood through the topographic
imaging of the sample with nanometer resolution via AFM (Atomic Force Microscopy) where
the topography maps depend on the tip-sample interaction. In order to achieve this goal first
we need to optimize the surface to obtain clear AFM images at high resolution. Then we will
make different experiments in air and liquid to study the interaction of DNA with protein
complexes, including MCM complex. Next we plan to perform AFM measurements at
different temperature, to confirm the presence of an origin: origins in fact share some
89
common characteristic for example A-T richness that causes a structural instability of the
DNA by increasing the temperature. The A-T bonds will in fact break earlier than G-C (two
hydrogen bonds instead of three). We also expect conformational changes that can be
determined by structural studies using AFM.
After getting interesting results for DNA-protein complex interaction we will try to study
more precisely cancer cells and understand more about the replication process in these cells.
Educational Activity foreseen
¾Molecular self-assembling and nanostructures, given by Lucia Pasquato, Alberto
Morgante and Loredana Casalis.
¾Other seminars and courses at UniTs, SISSA and ICTP.
90
ALESSIA AMODIO
Title of the thesis: Quantitative Analysis of Tumor Suppressor and Oncogene Proteins in
Tumor Micro-Samples
Laboratory: Laboratorio di Nanomedicina, S.O.C. di Farmacologia Sperimentale e Clinica,
Centro di Riferimento Oncologico (CRO), Aviano; Laboratorio di Chimica Analitica Università Tor Vergata di Roma
Supervisor: Dr. Giuseppe Toffoli
Tutors: Dr. Matteo Castronovo, Dr. Francesco Ricci
To develop novel, effective treatments for cancer patients, the experimental clinical research
is certainly bound to the ability to detect, characterize and understand the molecular basis of
cancer development and progression. The cellular heterogeneity in tumour samples, patients’
diversity and their different response to therapies are among the several factors that
unpredictably bias experimental results found in different laboratories. For instance, laser
micro-dissection (LCM) seems to properly address the issue of tissue heterogeneity and
represents the state-of-the-art in reducing biological noise that bias biomarkers identification
[Cadron I. et al., Gynec. Onc., 2009]. High-throughput evaluation of biomarkers in tumour
micro-samples, however, is particularly hampered by the lack of sensitive analytical tools
[Nan Y. et al., Med. Oncol., 2009].
For instance, currently available methods to analyze protein-nucleic acid interactions within
biological samples mostly rely on one or more purification/separation steps that require
>0.1mL sample amounts, and, in addition do not allow detection of biomolecular complexes
under equilibrium conditions (as they might form in the cell). These critical aspects reduce
our capability to quantify protein-nucleic interactions in biological micro-samples (i.e. in µL
amounts) such as those that can be obtained from early tumours or LCM samples. Basic
research has identified a number tumour suppressor or oncogene proteins among transcription
factors [Darnell J.E. Jr, Nat Rev Cancer, 2002] that are sequence-specific, DNA-binding
proteins that regulate gene expression [Latchman D.S., Int. J. Bio. Chem. Cell. Bio., 1997].
Nanotechnology overcomes the aforementioned analytical limitations with the possibility of
measuring protein amounts down to a hundred molecules and sub-nanomolar concentrations,
and in nanoliter to picoliter volumes [Chiu D.T. et al., Acc. Chem. Res., 2009], that are
compatible with the aforementioned tumour micro-samples. Furthermore, electrochemical
analysis is one of the most sensitive methods for detecting biologic substances [Li J. et al.,
91
Electroanalysis, 2012] and can be used to detect cancer biomarker [Wang J., Biosens.
Bioelectron, 2006; Chikkaaveeraiah B.V., ACS Nano, 2012].
Recent years have seen the development of a broad class of electrochemical biosensors that
employ a redox-tagged DNA probe that is covalently attached to an interrogative electrode
(hereinafter E-DNA). The detection signal is produced by a binding-induced change of the
efficiency by which an attached redox tag approaches the electrode surface. Such sensors
perform well in complex media, as e.g. crude cellular extracts and undiluted blood serum,
because the inherent binding-induced changes are generally not mimicked by the nonspecific
adsorption of interferants to the electrode surface, and also electroactive contaminants are rare
in biological samples.
For these reasons this kind of biosensor has the potential to provide fast, highly sensitive and
economic detection, to be used in medical diagnostics.
The objective of this project is to develop and apply an easy-to-use, portable sensor for the
functional analysis and detection of tumour suppressor and oncogene proteins in tumour
micro-samples with label-free, electrochemistry-based devices. Also, in this project I plan to
develop specific protocols for analyzing biological samples with increasingly reduced volume
with the proposed approach.
The aim of the first year is the development of useful “signal-off”3 and “signal-on”4 probes.
To test the feasibility of the sensing systems, control experiments will be performed under
different conditions (e.g., pH, ionic strength, frequency), these experiments will also allow to
optimize the experimental parameters in order to achieve the best sensing performance.
The aim will be reached through these steps:
•targets identification and selection;
3
“Signal-off” E-DNA. In the absence of a target protein, the probe-attached, redox tag is free to collide with the electrode
surface and, in turn, a large faradaic current is produced at the redox potential corresponding to the redox tag. In the presence
of a target protein, the faradic current is suppressed upon recognition between target and probe. The latter leads to a “signaloff” behaviour, which allows target detection [Ricci F. et al., Anal. Chem., 2009].
4
“Signal-on” E-DNA. The inherent, novel sensor relies on a probe structure-switching mechanism. Specifically, the probe is
an electrode-bound, redox-tagged DNA forming two, stable, and rapidly interconverting conformations (nanoswitch)
[Bonham A. et al., J. Am. Chem. Soc., 2012]. In one state, a redox-active reporter is located adjacent to the electrode, thereby
leading to an efficient electron transfer. In the other state (the nonbonding state), the redox label is located far away from the
electrode, thereby reducing the electron transfer efficiency. In absence of the target, the nonsignalling, nonbinding state is
thermodynamically favoured. On the contrarily, in the presence of the target, the conformational equilibrium is shifted,
thereby favouring its target binding-competent state. The inherent, structural remodelling of the E-DNA probe corresponds to
a large increase of faradaic current through the electrode, which allows the “signal-on”-based, target detection.
92
•probes design;
•electrochemical analysis with different experimental conditions;
•electrochemical analysis with different nanoswitches;
•Atomic Force Microscopy experiments with experimental conditions;
Research project
To achieve the objective of my PhD project both “signal-off” and “signal-on” E-DNA probes
will be implemented. In order to develop E-DNA, targets will be selected and probes will be
designed (a nucleic acid folding and hybridization prediction program, such as Mfold, will be
used in order to construct sequences and to have information about several features of the
probes such as free energy estimation of specific conformations of the probes).
In order to demonstrate the sensing principles, I will first use conventional macro-electrodes.
To select the best experimental parameters and evaluate the analytical performance of the
molecular switches, I will perform a series of electrochemical analysis (using an Autolab) of
purified solutions modifying the experimental parameters, using different nanoswitches,
redox labels and novel nanoelctrode materials.
For each trial probe, calculated and experimentally derived values of Ks will be
systematically compared. To determine the switching equilibrium constant of each variant, I
will use urea denaturation.
AFM will be used to investigate the mechanochemical properties of the surface-bound
biomolecules, and their influence on the behaviour of the electrochemical sensors and to
monitor the formation of probe-target molecular complexes in a label-free manner, and
directly on the surface of the electrode (without requiring its operation) as a function of the
aforementioned surface properties.
The E-DNA sensors thus developed and optimized will be adapted to a multi-array platform.
We will couple these arrays with portable instrumentation (such as PalmSens) and low-cost
gold screen-printed electrodes.
Results obtained with electrochemical DNA-based sensors will be calibrated by means of
currently available technologies (e.g. ELISA, Western-blot experiments). This calibration step
will be also undertaken to demonstrate the advantages and the limitations of our approach.
A tentative project Gantt diagram is shown below.
93
Activity
Task
1
1. Development of “signal-on” and “signal-off” probes
2
3
4
5
First Year
Second Year
Month
Month
6
1.1 targets selection
1.2 probes design
2. Best experimental parameters selection and
analytical performance of the molecular switches or
Sensing systems testing, evaluation and improvement
2.1 electrochemical analysis of purified solutions with different
experimental parameters
nanoswitches
redox labels
2.4 electrochemical measurements with
novel nanoelectrode materials
3. Dissociation constants (Ks) determination
3.1 Binding curves
4. Switching equilibrium constants determination
4.1 Urea denaturation experiments
5. Effect of surface confinement
5.1 AFM experiments
6. Analysis of complex biological samples
6.1 Procedures development
6.2 Electrochemical measurements
7. Electrochemical analysis of
complex biological microsamples
7.1 Screen-printed electrodes fabrication
7.2 E-DNA sensors adaptation to to a multi-array platform
7.3 Microsamples preparation
7.4 Electrochemical measurements
8. Calibration of the obtained results by means
of currently available technologies
8.4 ELISA experiments
8.5 Western-blot experiments
Figure 1 – Gantt diagram
94
7
8
9
10 11 12 13 14 15
14
17
18
19 20 21 22 23 24 25
Third Year
Month
26
27
28 29 30 31 32 33 34
35
36
I will improve my knowledge in the field of E-DNA by studying literature about their
development, application and characterization and by taking part to meetings, seminars and
courses.
At the moment I plan to participate to:
-Interdisciplinary spring school (7-8 March 2013, Udine)
-Summer school on nanotechnology (usually first week of July, Trieste)
Until now I participated to the Annual Meeting of PhD School in Nanotechnology (9-11
January 2013) and I’m attending an English course at UPTER- Rome.
95
FRANCESCA CAMMISULI
Title of the thesis: Effects of nanomaterials on biological barriers fetal and post-natal and
toxicity evaluation epigenetic.
Laboratory: Laboratorio integrato di immunopatologia - I.R.C.C.S. Materno-infantile «Burlo
Garofolo» di Trieste
Supervisor: Pascolo Lorella
Nanoscience is a result of scientific progress in the twenty-first century. The diffusion of the
studies on nanomaterials and nanotechnology appears to be growing. Nanotechnology, in fact,
represents a great opportunity for development. Nanomaterials represent a significant
opportunity for innovation that is covering across many areas ranging from medicine,
electronics, energy production. To date, this science has provided remarkable results. The
utility of nanostructures and nanomaterials is linked to their particular flexibility.
Beyond the merits and the substantial opportunities offered by nanotechnology research,
however, it is important to focus on another aspect of the research: that is the potentially
associated risk for health. There is a great need of studies aimed at understanding the possible
harmful effects of nanomaterials. People are exposed to nanomaterials from many sources
from final products at manufacturing processes. In some working environments, certain
nanoparticles can be inhaled by workers and there is a risk that they can act such as asbestos.
Many scientists argue that nanomaterials could put the health of people at risk, causing
inflammation and possibly cancer.
From the actual definition, nanoparticles (NPs) are structures of various shapes and different
composition with the size ranging 1 and 100 nm. They are divided in NPs of natural origin
(produced by combustion as in volcanoes), NPs of anthropogenic origin (produced by diesel
engines or industrial incinerators) and artificial NPs (obtained through nanotechnology).
These structures possess unique physical and chemical properties, which depend on their
nanoscale dimensions and especially on the high ratio surface area/volume, that give to the
NPs peculiar chemical reactivity, optical, magnetic, catalytic and electrochemical properties.
96
In the last decades, these characteristics have made the new NPs of considerable interest in
technological development and are widely used in medicine and diagnostics, in biotechnology
and in cosmetics, food and materials. However, the increasing exposure to nanoscale particles
requires studies in order to characterize the properties and potential toxic effects. Although
they are applied in a vast number of fields that seem to be destined to increase, the effect of
exposure in humans remains undetermined. From the first studies, it seems that some NPs
may lead to in vitro alteration of gene expression and cell death and that they are able to
induce DNA damage both directly and indirectly, causing oxidative stress and inflammatory
responses. Although many suppositions have been made about the possible harmful effects of
nanoparticles on the body, it is not clear yet what is the exact mechanism by which these
nanostructures interact with cells and subcellular structures.
My Ph.D. work is part of the project that aims to unravel the possible effects and the toxicity
induced by nanomaterials. In particular my project will deal with the effects of exposure
during fetal and post natal life. The work will concentrate on the ability of the placenta and
other biological barriers to prevent nanoparticle diffusion into the body. At the same time
both short term effects (at barrier level) and long term effects (DNA damage) will be
investigated. Appropriate cell models will allow to investigate the relationship between
physico-chemical properties and toxicity. I’ll particularly concentrate on the possibility to use
unconventional approaches to reveal unknown features of effects of nanomaterials in
biological systems.
1.Evaluation of the toxicity at tissue level in human placental and other physiological
barriers (lung, brain):
Collection and classification of human tissues (human at term placentas from
donors, other tissues from archives and biobanks) of patients exposed to
nanoparticles.
Investigation for the possible presence, histological localization and chemical
characterization of nanomaterials on biological barriers (mainly placenta and
lung) trough conventional and innovative microscopic techniques based on the
synchrotron radiation.
97
Correlation of multiple approaches and techniques (conventional and advanced
techniques, molecular and physico-chemical analyses) to evaluate the toxicity
of nanomaterials on biological barriers.
Use of molecular techniques to evaluate the toxicity due to epigenetic effects of
nanomaterials at the DNA.
2.Evaluation of the toxicity in models of biological barriers (placenta/lung/brain) by in
vitro studies with cell lines of human chorion carcinoma (BeWo,Jar,JEg-3) and others.
Identification of cellular targets, and sub-cellular and molecular mechanisms of
toxicity of nanomaterials using conventional and innovative microscopic
techniques.
Investigate if the chemical-physical characteristics of nanomaterials and their
reactivity are translated into special effects and biological mechanisms.
3.Search of epigenetic effects due to the toxicity of nanomaterials:
Assessment of molecular and innovative microscopy to evaluate the toxicity due
to epigenetic effects of nanomaterials on the DNA.
4.Potential validation of new protocols for the study of toxicity of
nanomaterials.
Assessment of epigenetic toxicity evaluating modifications connected to the microenvironment that alter the degree of activity of genes without modifying the gene
sequence. One of the epigenetic mechanisms from exposure to environmental agents is
DNA methylation.
Assessment of microscopic techniques of vibrational (IR-FTIR/ IR-RAMAN) to
detect macromolecular changes in biological systems.
Set up of molecular techniques to detect the methylation at the DNA by
bisulphate sequencing.
Assessment of
molecular techniques to evaluate the alteration of gene
expression by DNA microarrays technology.
5. Integration of different data and risk assessment of the exposure to nanomaterials
(mainly ambiental) during pregnancy and in post-natal life.
Collection and classification of human tissues (human at term placentas from donors,
other tissues from archives and biobanks) of patients exposed to nanoparticles.
98
Evaluation of the toxicity in models of biological barriers (placenta/lung/brain) by in
vitro studies with cell lines of human chorion carcinoma (BeWo,Jar,JEg-3),
identifying the cytotoxic and genotoxic effects.
Development of multiple approaches and techniques (conventional and advanced
techniques, molecular and physico-chemical analyses) for the accurate evaluation of
the characteristics of toxicity nanomaterials on biological barriers fetal and post-natal.
Utilization of microscopic innovative techniques based on the synchrotron radiation
(XRM-XRF-XRD) to assess the presence and morphology of nanomaterials in
biological systems model.
Development of protocols with microscopic techniques of vibrational (IR-FTIR) to
detect changes in biological systems at the macromolecular model.
Development of protocols with molecular techniques to evaluate the toxicity due to
epigenetic effects of nanomaterials at the DNA (ie. methylation)
Development of protocols with microscopic techniques of vibrational (IR-Raman) to
analyze macromolecular changes at the DNA (ie. methylation).
Validation of these new techniques for the study of toxicity.
Research project
The project implies the following activities:
1.Sample selection and identification.
Collection of tissue samples (biological barriers fetal and post-natal) from
persons exposed in areas of interest and classification according to the hazard
of exposure.
Extraction of DNA from tissues and classification according to the hazard of
exposure of the subject to nanoparticulates.
Identification and selection of control samples: DNA control / control tissues
(other biological barriers, for example the lungs or the blood-brain).
2.Sample preparation: cellular models.
Models of toxicity (placenta/ lung / brain), with particular reference to the
chorion carcinoma cell lines of human (BeWo, JAr and JEg-3).
99
Study of the cellular localization of nanomaterials and on effects of exposure to
various concentrations of nanomaterials model by conventional and innovative
microscopy.
−Conventional microscopy: optical microscopy based on color "vital" and
"non-vital" to reveal morphological details otherwise not visible or
indicate the metabolic state of the cell, thus determining cell vitality.
−Fluorescence microscopy: for evaluation of whether the nanoparticles
induce cell death by apoptosis; the cell lines will be analyzed by
fluorescence microscopy after fixation with the nuclear dye DAPI to
highlight the nuclear morphology and the presence of apoptotic bodies.
−Innovative microscopy:
xX-ray microscopy to study the morphology of the sample.
xX-ray fluorescence to identify and quantify the chemical elements
present in the sample.
xX-ray diffraction for determining concentration profiles, film
thicknesses, the atomic arrangement in amorphous materials and
multilayer.
3.Microscopic analysis of tissue.
Conventional microscopy: histochemical stains to localize the presence of
transition metals (i.e Fe) and discriminate the oxidation state in regions of
tissue that incorporated nanoparticles.
− Hematoxylin and eosin staining to analyze the morphology of the tissue
and the presence of any nanoparticles.
− Perls/ Lillie/ Schmorl staining to discriminate the different oxidation
states of iron ion.
4.Analysis of histological samples processed by optical microscopy.
Innovative microscopy analysis exploiting the synchrotron radiation: XRM-XRF
analysis of histological samples to study with better resolution the morphology
of the tissue sample, and simultaneously to identify and quantify the chemical
elements present in the sample; the data will be analyzed by appropriate
software (ie. PyMCA).
100
5.Analysis of epigenetic changes.
Microscopic techniques of vibrational (IR-FTIR) to detect changes in biological
systems at the macromolecular model.
Microscopic techniques of vibrational (IR-Raman) to analyze macromolecular
changes at the DNA (ie. methylation or phosphorylation).
Molecular techniques to evaluate the toxicity due to epigenetic effects of
nanomaterials at the DNA (ie. methylation).
−With the technology of DNA microarrays it is possible to measure the
level of expression of thousands of genes simultaneously, using the
basic principles of hybridization of nucleic acids: microarray
experiments will allow to understand which genes are modulated by
methylation as the effect of nanomaterial presence.
−Analysis of DNA methylation using bisulphite sequencing: The method is
based on the deamination of cytosine residues to uracils in the presence
of NaOH and sodium bisulfite. Since methylcytosine is not converted
under these conditions, the original methylation state of the DNA can
be analyzed by sequencing of the converted DNA. After the conversion
reaction, the DNA sequence under investigation is amplified by
polymerase chain reaction (PCR) with primers specific for one strand
of the bisulfite-converted DNA. The PCR product is cloned and
individual clones are sequenced.
6.Integration of data obtained with different microscopy and molecular techniques .
101
Tasks
1.
Sample
selection
and
identification
2. Sample preparation: cellular
models
3. Microscopy analysis of tissue
4. Analysis of epigenetic changes
5. Integration of the data obtained
Start
month
Duration
(months)
End month
mar-13
12
mar-14
apr-13
giu-13
mag-13
dic-15
21
32
38
10
gen-15
mag-16
lug-16
set-16
For the first year I plan to follow one or two courses at University:
xOptical spectroscopies applied and analysis of images
xIntroduction to organic spectroscopy.
102
CANDIDATE PIETRO CAPALDO
Title of the thesis: “Capacitance Immunosensors for the early detection of circulating cancer
biomarkers”
Laboratory: NanoInnovation Laboratory at Elettra-Sincrotrone Trieste
Supervisor: Dot.ssa Loredana Casalis
Medical diagnosis requires point-of-care biosensor arrays at the patient's bed. This
requirement is due to new emerging demands for personalized therapies because therapeutic
agents amount in tissue and blood serum is different on a patient-by-patient basis. Therefore,
the development of low-cost, point-of-care technologies for array biochips is a necessary step
to introduce personalized therapies in clinical practice and the miniaturization, developed in
recent years, of biosensors and their integration in microarrays and functional biochips will
enable massive parallel detection of analytes, diseases and disease susceptibilities, as well as
identification of personalized drug response profiles. The concept of a “Micro Total Analysis
System” (mTAS), where attempts are made to completely integrate (bio)chemical systems on
silicon or glass substrates, has been envisioned as a new concept for analytical devices,
because it is expected that miniaturization will improve the speed and reliability of the
measurements and will dramatically reduce the sample volume and the system costs.
The gold standard for single protein measurements is immunoassay, which exploits the
specificity and sensitivity of antibody-antigen recognition, like in the Enzyme-Linked
Immuno-Sorbent Assay (ELISA), which has a well-established specification for measurement
of single proteins in a solution, with a lower limit of reliable quantitation of ~1 pg/ml and
dynamic range of 3 logs. Such devices can assay up to 16 different sample in some
commercial platform, like Pierce Biotechnology, in few hours [S. Kingsmore, Nat. Rev. Drug
Disc., 5, 310 (2006)]. Also, protein array technology has evolved and found its way into
quantitative proteomics through the construction of what is called a “protein
microarray”.[K.D. Kumble, Anal. Bioanal. Chem., 377, 812 (2003)] based on optical
detection, such as fluorescence [B.B. Haab et al., Genome Biol., 2, RESEARCH0004 (2001);
H. Zhu et al., Science, 293, 2101 (2001)], or SPR (Surface Plasmon resonance) [C.T.
Campbell and G. Kim, Biomaterials, 28, 2380 (2007); J.M. McDonnell, Curr. In such optical
sensors the target molecules can be labeled with fluorescent tags such as dyes and the
fluorescence is detected in presence of the targeted molecule. This allows for extremely
sensitive detection down to a single molecule. However, the process is laborious and may also
affect the function of the biomolecules.
In label-free techniques, proposed as alternative to fluorescence and chemi-luminescence, the
targeted molecules are detected in their natural form. These molecules are attached to the
optical sensor surface using probe molecules and the attachment is detected by measuring the
change in optical properties for the sensor. In particular, sensors based on silicon-on-insulator
(SOI) photonic wire waveguides are important because of their compatibility with CMOS
fabrication and possibility of integrating detection and decision on the same chip leading to
“laboratories on a chip”. Further, guided-wave sensors allow for integration of multiple
sensors on a single chip. But, the considered micro-array technology based on optical
103
detection and molecular labeling is costly and time consuming. Thus, it is not adapted for
applications to personalized therapy in hospital or at home.
The aim of the present research project is to develop a successful design for a point-of-care
immuno-sensor, optimized in terms of simplicity, reproducibility, sensitivity, and cost. Our
idea is to perform electrochemical impedance measurements in microfluidic channels, where
the electrodes are properly functionalized with biomarker-specific capture proteins to allow
for a label-free detection of fM concentration of biomarkers in small biosample volumes
The final goal of my PhD is to create a microfluidic immunosensor based on the electrical
detection on array of a series of parallel microfluidic channels (four or five), in other words a
multiplexing system, where each channel is characterized by a series of bio-functionalized
microelectrodes, thus providing the possibility to recognize 4 or 5 biomarkers simultaneously.
¾The first year will be, almost entirely, spent in learning different techniques of microfabrication and (bio)functionalization of the electrodes so constructed. In particular,
talking of micro-fabrication, I should learn to use the basic equipment for the
manufacture of electrodes. Below is a list instruments that should be used and its
function:
Spin coater: It is used to cover a sample with a uniform thin film. An excess amount of
resist is placed on the slide which is then rotated at high speed in order to spread
the fluid by centrifugal force.
Mask Aligner: It uses light to transfer a geometric pattern from a photo-mask to
a light-sensitive chemical photoresist, or simply "resist," on the substrate.
Electron Beam Evaporator: is a form of physical vapor evaporation in which a target
(in our case Ti and Au) anode is bombarded with an electron beam produced from
a hot tungsten-filament under high vacuum. The electron beam causes atoms from
the target to transform into the gaseous phase. These atoms then precipitate into
solid form, coating our samples in the chamber with a thin layer of the anode
material.
Atomic Force Microscopy (AFM) to check and optimize the biofunctionalization
protocols.
Set-up of the read-out system, based on a HEKA Potentiostat/ Galvanostat PG 340
usb, for the detection of 1 reference biomarker.
Calibration of the capacitance read-out of 1 single channel device, compared to
standard ELISA and nano-arrays (developed in our laboratory) outputs.
Research project
We plan to develop a new class of immune-sensors where antigen-antibody interactions are
detected via impedimetric detection as for Electrochemical Impedance Spectroscopy (EIS). In
other words, the signal in this type of sensors depends on the impedance change produced by
104
the measured quantity in the geometry of an electrical equivalent circuits (EECs) [Figure 1]
related to electrochemical cell constant, and thus the change can be used for the sensing
mechanism of biological species. To better understand the application of a EIS microfluidic
chip, try to define its structure. In particular, we discuss the alternating current (AC)
frequency characteristics of these sensors as parameters for sensor design, such as
interelectrode spacing and electrode area.
Consider a glass device [Figure 2 (c)], which consisted of a capillary channel and coplanar
impedance sensors. This latter feature is important because coplanar electrodes can be easily
patterned at very small dimensions which allows to build miniaturized and low cost devices.
Design parameters are as shown in Figure 2 (d).
In this context, Rsol represents the properties of the electrolyte solution and the diffusion of the
redox probe. If two electrodes of equal area S [m2] are placed in parallel at a perpendicular
distance D [m] from each other, the solution resistance Rsol [W m], measured by driving the
electrodes with an AC voltage v [V] and by measuring the resulting current [A], is related to
the solution resistivity rsol [W m]. A parasitic resistance, RPara, is present in series with the
sensor and it resulted from the resistance in the connecting cables and mainly Au (or Pt) thin
film electrodes.
Direct capacitive coupling between electrodes is represented by the cell capacitance, CCell,
whose value is determined by the dielectric constant of the electrolyte and the geometry of the
electrodes.
The coplanar electrodes are usually embedded in dielectric substrates (glass) and the only
small area of electrodes contacts with biomolecule solutions for sensing and so, the parasitic
capacitance due to dielectric substrates cannot be ignored. Thus, the parasitic capacitance
must be reduced to enhance the sensitivity. Additionally, a metal electrode in contact with an
electrolyte possesses a natural charge density due to an excess or a deficiency of electrons at
electrode-electrolyte interface, in order to maintain electrical neutrality. This accumulation of
ions forms an electrical double layer that consists of an adsorbed fixed layer, which is
independent of electrolytic (ionic)concentration, and a diffusive mobile layer, which is a
function of the ionic concentration. This electrostatic phenomenon is electrically represented
by a double-layer capacitance, CDL .
The interfacial capacitance (of the level nF to mF) can be used as a sensing mechanism
combined with specific surface reactions. When antibodies bind to antigens, the change in the
electric properties results in a change of impedance, enabling the measurement of direct
electrical signal, such as magnitude and phase angle and the surface modification may be
accompanied to improve the sensitivity and selectivity.
105
Fig.1.
(a) An electrified interface in which the
electrode is negatively charged. (b): An
idealized electrical equivalent circuit for the
interface.
Abbreviations:
IHP
inner
Helmholtz plane; OHP outer Helmholtz
plane.
Fig.2.
Schematic diagram of a glass-based
micro-channel chip with coplanar
impedance sensors:
(c) layout of a glass device and (d)
rectangular planar electrodes.
(e) Schematic diagram of total
Impedance frequency plots divided into
dominant components.
An EIS microfluidic chip so constructed and with a particular detection technique allows the
identification of various biomarkers of interest within a fluid/serum such as may be human
blood. So, the next step, in the chip development, will be characterize the electrode of the
chip, i.e. design and develop innovative biosensors for the routine detection of extremely
small amounts of genetic material. In this context, a novel approach for the functionalization
of the electrode of the EEC, which takes advantage of AFM-based nanografting, is the SelfAssembled Monolayers (SAMs) of organic molecules. And, it is thanks to this nanografting
technique that I can characterize the electrode in my EEC and therefore can make
measurements of electrochemical impedances very sensitive to certain biomarkers.
106
•Molecular selfassembling and nanostructures, given by Lucia Pasquato, Alberto
Morgante and Loredana Casalis.
•A course focused on learning of system design platform LabView, given by Dr. Cautero at
Elettra-Sincrotrone.
•Other seminars-conferences held in Trieste, such as the training course, given by the
Prof. Scoles, on intermolecular forces.
107
LUCIA CORAL
Title of the thesis: Detection of tumor cell surface biomarkers in microsamples
Laboratory: Centro di Riferimento Oncologico di Aviano
Supervisor: Giuseppe Toffoli
Tutors (if any): Matteo Castronovo, Giacinto Scoles
Cancer research requires a truly accurate evaluation of biomarkers that yet have not been met
due to several factors that unpredictably bias experimental results found in different
laboratories such as the different developmental stages of tumor and the cellular heterogeneity
in tumor samples beside patients’ diversity and their different response to therapies.
Nanotechnology-based approaches to protein detection can be more sensitive than ELISA
(see for instance Nanosphere – Gold Nanoparticle technology). Several ELISA variant have
been developed and 0.01 pg/mL sensitivity have been demonstrated (see High Sensitivity
ELISA Kits commercialized by eBioscience). ELISA, however, is based on enzymatic
reactions that have a limited application to the analysis of solid samples, and in vivo. For
instance, fluorescence activated cell sorting (FACS) analysis of cells marked with
fluorescently labeled antibodies is one of the most commonly used methods for analyzing cell
surface protein biomarkers. However, this technique can be used only for studying highly and
moderately expressed biomarkers (102-104 proteins per cell are required) (Joensson H.N. et al.
Angewandte Chemie Int. Ed. 2009). Chemiluminescence is the detection methods of choice
for Western-blot analysis. This approach however provides semi-quantitative results and is
based on enzymatic reactions. Furthermore, in general, quantitative gel analysis is performed
by using the less safe immuno-radioactive labeling approach.
In the last five years, DNA self-assembly has been used to generate molecular nanodevices
where biomolecules are confined with controlled molecular packing, composition and
chemical functions, and allowed the implementation of unprecedented detection approaches
for analyzing biomolecules in small sample-volumes (potentially down to single cell
amounts). Water-soluble, dense, DNA nanostructures (also called ‘DNA-origami’) were
introduced by P.Rothemund in 2006, who provided a software tool to design planar shapes
that form via Watson-Crick hybridization of several, short single strand DNA (ssDNA)
sequences (the staples) with a long circular ssDNA molecule (the scaffold) (Rothemund P.
Nature 2006). Since then, several papers demonstrated the functionalization of DNA-origami
with different probes among which antibodies for immuno-detection (Douglas S.M. et al.,
Science 2012). DNA self-assembly allows precise spatial positioning of attached probes:
108
P.Tinnefeld and co-workers demonstrated that the precise localization of fluorophores on a
two-dimensional DNA-origami breadboard allows for alternative energy-transfer pathways
dependent on the incorporation of a “jumper” dye at specific positions (Stein I.H. et al.,
JACS, 2011). S.Lindsay and co-workers demonstrated that DNA-origami display enhanced
resistance to enzymatic digestion in complex biological mixtures such as cell lysate at
different incubation times, temperature and concentrations, with respect to unfolded scaffolds
(Mei Q. et al., Nano Letters 2011).
A major limit to the widespread application of DNA-origami in biomedical research is
provided by their relatively high cost. M-13 ssDNA-based DNA-origami can be conveniently
amplified by e.g. phage display, and also other scaffolds can be used (Castro C.E. et al.,
Nature Methods 2011; Högberg B. et al., JACS Communications 2009). In addition, ssDNA
engineered staples could be amplified after being incorporated in DNA vectors, and extracted
from these latter via restriction enzymes processing.
The major objective of this short-range project is to develop low-cost, programmable probes
for the highly sensitive immunodetection of cell surface, tumour biomarkers in microsamples, based on fluorescence optical imaging. The goal is also to limit sample preparation
as to reduce sample degradation. In the long range, this approach will allow quantify amounts
of candidate, innovative biomarkers in heterogeneous tumour samples, where they are
scarcely expressed. My work will comprise:
-development of low-cost, water-soluble DNA nanostructures, functionalized with 10-100
copies of a fluorescent molecules for signal amplification;
-linkage of these nanostructures to probes (e.g. DNA aptamer, or monoclonal antibody)
that are specific to the pre-selected, cell surface biomarkers;
-calibrating this novel quantitative approach with simplified samples such as decreasing
volumes of standard solutions containing the target protein or cultured tumour cell
lysates, for comparison with ELISA assay, Western Blot assay and FACS;
-quantification of amounts of cells-surface biomarkers by means of fluorescence optical
imaging within different type of samples. These include, in particular, ex-vivo,
unprocessed, solid tumour tissues with sizes ranging from a few cm (corresponding to
volumes of a few mL) down to a few hundred μm (corresponding to volumes of a few
μL);
-developing some simple protocols for reducing the cost of synthesis of these DNA
nanoassemblies and enabling high throughput applications.
For the first year I plan:
-to synthesize and purify already known DNA nanostructure;
109
-to label DNA nanostructure starting from published shapes with several copies of a
fluorescent dye (to obtain so called ‘OriFluo’ complexes);
-to label OriFluo with antibodies directed against a tumour cell surface biomarker (to
obtain so called ‘Ab-OriFluo’ complexes);
-to perform exploratory test of Ab-OriFluo in calibration samples;
-to amplify M-13 ssDNA scaffold by cultured cells transfection.
Research project
Synthesis of Fluorescently labelled DNA-origami linked to an antibody (Ab-OriFluo).
DNA-origami will be designed by using the online-available software called CadNano 2.
Folding of DNA-origami will be obtained by hybridizing the scaffold (e.g. M-13 ssDNA, 130 nM) and ss-oligonucleotide staples in a 5x excess during a thermal ramp (typically:
T=95°C for 15min, followed by cooling at -1°/min, up until T=20 °C is reached). Different
buffers will be used. Typically M-13 based DNA-origami properly folds in 10mM Tris, 12.5
mM MgCl2, pH 8.0. Agarose gel electrophoresis analysis will be used to monitor the weight
of the obtained DNA-origami as to select best synthesis conditions.
DNA-Ab likage. Solulink provides a kit to directly link an antibody to an oligonucleotide
through two organic linkers (sulfo-S-4FB e S-HyNic) without needing HPLC purification.
The extraction of the complex is made by conjugate adsorption on magnetic affinity beads.
DNA-fluorophore linkage. Staples modified with organic fluorophore can be purchased
from almost any manufacturer (e.g. Integrated DNA Technologies – IDT). Otherwise, (thiol /
amine) modified fluorophores can be linked to (amine / thiol) modified staples via sulfoSMCC etherobifunctional crosslinker (Pierce). In general each copy of a DNA-origami based
on the M-13 ssDNA scaffold contains about 200 staples. Therefore, compared to
commercially available, fluorescently labelled antibodies, I expect Ab-OriFluo to provide
fluorescence intensities two order of magnitude higher.
Analysis of Ab-OriFluo. I plan to use AFM to analyse the structure of obtained DNA
nanoassemblies
adsorbed on a mica surface (Rothemund P. Nature 2006). Also, side-by-side, AFM
topographic images will be used to detect antibodies linked to the DNA structure as their
presence produces a significant height increase. Fluorescence, optical imaging of the obtained
DNA-origami adsorbed on a fused-silica support will be carried out to determine the
functionality of the DNA-attached fluorophores. A transmission electron microscopy-based
analysis will be needed to provide structural characterization of the DNA-origami. Dr.
Castronovo and I are working to develop specific collaborations with researches in EU or
USA for this.
110
Purification of Ab-OriFluo. Samples need to be purified as to remove the unbound
fluorescently labelled staples. Different technique will be tested in order to obtain high
recovery yield and low degradation of the structures.
Development of low-cost amplification method of ssDNA scaffold for Ab-OriFluo
synthesis. In contrast to most other bacteriophages, which assemble inside the cytoplasm, and
are released by cell lysis, the
morphogenesis of filamentous bacteriophage (M-13) is
simultaneous to virus extrusion, without inducing cell lysis. Infected cells continue their
growth and divide while extruding progeny phage particles into the medium. In turn, I plan to
produce high amounts of M-13 in cell cultures by standard precipitation by adding PEG to
culture medium.
Development of low-cost amplification method of ssDNA staples for Ab-OriFluo
synthesis. I plan to engineer a phage vector that contains several staples, which are connected
by spacers, each one containing a restriction site.
111
GANTT DIAGRAM
Year 1 I trim. II trim. III trim. IV trim.
Year 2 I trim. II trim. III trim. IV trim.
Year 3 I trim. II trim. III trim. IV trim. Synthesis of Fluorescently labelled DNA‐origami linked to an antibody (Ab‐OriFluo) Developing a method for the synthesis and purification of DNA‐origami with known or novel structures Labelling of the DNA‐origami with antibodies directed against a cell surface tumour biomarker and caractherization of the obtained structure (Ab‐Ori) Linkage of several copies of a fluorescent dye to the DNA‐origami and characterization of the obtained structure (OriFluo) Labelling of the OriFluo with antibodies directed against a cell surface tumour biomarker and caractherization of the obtained structure (Ab‐OriFluo) Exploratory test of Ab‐OriFluo in calibration samples Calibrating Ab‐OriFluo with standard solutions containing the target protein and sensitivity comparison with ELISA and Western Blot assays Calibrating Ab‐OriFluo with lysates of tumor cell cultures of decreasing volumes and sensitivity comparison with ELISA and Western Blot assays Application of Ab‐OriFluo in ex‐vivo samples Application of Ab‐OriFluo in cultured tumour cells and target tumour marker quantification Application of Ab‐OriFluo in solid tumour tissues and target tumour marker quantification Development of low‐cost Ab‐OriFluo Amplification of M‐13 ssDNA scaffold by cultured cells transfection Development of staples amplification methods Analysis of Ab‐OriFluo performances Structural characterization, for enabling data analysis Application of Ab‐OriFluo to micro‐samples of solid tumours 112
I will improve my knowledge in the field of DNA nanostructures by studying literature about their
synthesis and biomedical application and by taking part to meetings, seminars and courses.
At the moment I plan to participate to:
-Interdisciplinary spring school (7-8 March 2013, Udine)
-Summer school on nanotechnology (usually first week of July, Trieste)
-One of the following International conferences:
o“Self-Assembly & Supramolecular Chemistry” (Gordon Research Conferences), May
5-10 2013, Les Diablerets-Switzerland;
o“BioPhotonics & Imaging Conference BioPIC 2013” (The National Biophotonics and
Imaging Platform, Ireland), March 25-27 2013, Dublin-Ireland;
o“Nanomedicine 2013 (Select Bioscience), April 11-12 2013, Barcellona-Spain;
o“NanoBioeurope 2013 Conference” (NanoBioeurope), June 10-12 2013, ToulouseFrance.
So far I participated to the Annual Meeting of PhD School in Nanotechnology (9-11 January 2013)
and I’m taking an English course provided from CRO – Aviano.
CANDIDATE ILENIA D’AGOSTINO
Email: ILENIA.D’[email protected]
Title of the thesis: “Biopolymer based matrices for encapsulation and delivery of nutrients
typical
of the mediterranean diet.”
Laboratory: 244
Supervisor: Dott.ssa A. Gamini
Tutors (if any):
An increasingly important determinant in food choice is the growing consumer concern about
nutrition and health. This focusing on consumer interest on the food supply, and also extensive
research and technological developments in food science will provide further opportunities for new
products developments. The Food-based Dietary Guidelines of the World Health Organisation and
European Union legislation on health claims play an important role in regulating information about
a wholesome diet [1]. In food field there is an increasing attention towards use of specific
biopolymers to optimize the release of special nutritions. Food grate biopolymer such as protein and
polysaccharides can be used to create a diverse range of delivery systems suitable for encapsulating,
protecting, and delivering lipophilic functional components such as omega-3 rich oils, conjugated
linoleic acid (CLA), oil soluble vitamins, flavors, colors, and nutraceuticals. There are many
different approaches that can be used to create structured delivery systems based on biopolymers
including molecular complexation, coacervation, thermodynamic incompatibility, moulding and
extrusion method [2].
The research project is aimed to the preparation and the study of supramolecular systems made by
polymers, polymer particles or vesicles able to release proteins or other nutrients typical of the
Mediterranean diet. The apparent ability of the Mediterranean diet to reduce the risk of
development of cardiovascular disease, cancer and degenerative disease has been attributed in part
to the content of nutrients as α-tocopherol, ascorbic acid, retinol, β-carotene. The constant presence
of fruits and vegetables, virgin oil, red wine, aromatic herbs and garlic, onion and chilli, ensures the
intake of phenols, polyphenols, flavonoids, isoflavonoids, phytosterols, terpenes, monounsaturated
and polyunsaturated fatty acid. But there are any conditions in which there is a difficult to provide
an adequate nutrition as in case of intolerance or allergy towards a compound, in other situation of
stress or in particular case as in oldness in which there is a natural vitamins deficiency and a high
proteins-calories supply is necessary, or in specific disease in childhood. Particular attention will be
given to the use of biopolymer as matrices suitable for encapsulation of nutrients to improve their
absorption, bio-availability and environmental stability.
The first steps of the project will be addressed to the characterization of several types of
biopolymers like alginate, scleroglucan, chitosan or starch able to exhibit properties suitable for
nutrients loading and release. We will study the biopolymer-biopolymer interaction to check
Scuola di dottorato di ricerca in Nanotecnologie: attività 2013
114
thermodynamic compatibility since low mixing entropy of large size molecules is responsible for
their incompatibility in common solvents. The general condition for compatibility is an exotermic
mixing process i.e. the formation of soluble biopolymer-biopolymer complex [3]. There are many
numbers of factors that must be considered when selecting a suitable polysaccharide or a
combination of polysaccharides to fabricate a biopolymer-based delivery system. It is important to
establish the environmental and medium conditions favoring supramolecular structures formation.
This usually requires knowledge of physicochemical properties of the polysaccharides involved,
such as helix coil transition temperatures, electrolyte properties, sensitivity to specific monovalent
or multivalent ions or susceptibility to enzyme or chemical reactions [4].
In some cases is possible that a mixture of polymers causes a change on the behavior of the
components. This modification can active a process of sequestering essential components from the
solution in which polymers are immersed as we have observed in a mixture of chitosan and alginate
immersed in milk.
In fact an important aspect is the study of the specific interaction between polymers and active
molecules (nutrients) as proteins, peptides, vitamins and so on leading to stable polymer-nutrient
complex. Biopolymers in biological environment have to satisfy important requirements such as
biocompatibility and biodegradability as well as to be neither toxic nor immunogenic. Bioactive
compounds have different characteristics in terms of molecular structure, molecular weight and
polarity, ranging from polar to amphiphilic to non polar species.
Therefore the delivery system has a vital role in preventing and/or increasing the bioavailability of
an added bioactive compound. A delivery system must than protect the functional ingredient from
biological or chemical degradation that may occur during processing, storage, and consumption
(oxidation, hydrolysis); must as well control the release of the nutrient at the target site of the body
(pH, ionic strength, temperature) and be compatibility with the bioactive compound.
References:
1) Nehir El S. and Simsek S. Food Technological Applications for optimal nutrition:an overview of opportunities for the food
industry, Comprehensive Reviews in Food Science and Food Safety, Vol. 11, 2012
2) Matalanis A, Griffith Jones O. and McClements D. J. Structured biopolymer-based delivery systems for encapsulation,
protection, and release of lipophilic compounds, Food Hydrocolloids 25 (2011) 1865e1880
3) Tolstoguzov V. Why were polysaccharides necessary Nestlè research center
4) BeMiller, J. N., & Whistler, R. L. (1996). Carbohydrates. In O. Fennema (Ed.), Food chemistry. New York, NY: Marcel
Dekker, Inc.
The project is aiming to the development of suitable biopolymers based matrices for the up-take and
the delivery of specific nutraceuticals.
• Test and optimize the procedures to develop nano-gels, emulsions, polymer particles and
solid matrices exploiting polymer-polymer and polymer-nutraceutical interactions.
115
•
Characterize the systems in terms of size, structural inhomogeneities and physicochemical
properties.
• Study of the stability and the delivery properties of the developed systems as a function of
environmental conditions like temperature, pH or enzymes.
The first year is dedicated to a theoretical approach to the nanosystem and their application in food
field and a practical approach to the learning of the principal procedures and techniques for the
preparation and the characterization of the systems. The main objective is to select the biopolymerbioactive compound systems that show the most promising features in terms of stability,
compatibility and releasing ability.
Research project
The Project is based on the study of biopolymers behavior and their capacity to release nutrients.
The programme can be summarized in:
I.Characterization of the biopolymers and of their functionalized derivatives (DSC, viscometry,
spectroscopic techniques)
II.Study of the polymer-polymer and polymer-bioactive compound interactions. Study the thermal
behavior of polymers and their compatibility with nutrients (DSC, Surface Plasmon
Resonance, spectroscopic techniques, SAXS, NMR).
III.Development of biopolymer based nano- and micro-systems.
IV.Study of the physicochemical properties of the biopolymer-based matrices as their morphology,
physical properties, size and charge (as a function of pH, ionic strength, temperature)
because these factors will determine how the particles may impact stability and functional
characteristics (SEM, TEM, IR, NanoTracking).
V.Rheological characterization to study of the viscoelastic properties of the components.
VI.Study of the nutrient diffusion in the polymer network (spectroscopic techniques, NMR).
VII.Test of the different structures of the final products to select those that show good performances
in terms of environmental stability. The possible structures include polymer films (also
multilayer), scaffolds, polymeric particles (self assembled) and vesicles (or liposomes).
VIII. The study of the terms and conditions for the release of bioactive compounds (spectroscopic
techniques, HPLC, capillary electrophoresis.
116
Years1
Activities
3
6
Years2 Years3 9 12 15 18 21 24 27 30 33 36 LITERATURE RESEARCH
CHARACTERIZATION OF BIOPOLYMERS AND
NUTRIENTS
PREPARATION OF BIOPOLYMER MATRICES
RHEOLOGICAL CHARACTERIZATION OF
BIOPOLYMER MATRICES
FUNCTIONALISATION OF BIOPOLYMER
MATRICES
STUDY OF BIOPOLYMER-NUTRIENTS
INTERACTION
INSTRUMENTAL CHARACTERIZATION WITH
DSC, DLS, SAXS,SURFACE PLASMON
RESONANCE, NMR, RHEOLGY,
NANOTRACKING, IR, HPLC, UV-vis, CD
STUDY OF THE RELEASE OF BIOACTIVE
COMPOUNDS
PAPER WRITING
Courses :
•Chimica delle sostanze organiche naturali (Forzato)
•Biopolimeri (Rizzo)
Schools :
•Interdisciplinary Phd Spring School (7-8 March 2013)
•X Scuola di Reologia (8-12 September 2013)
117
CANDIDATE: MATTEO DE BIASI
Title of the thesis: Study of nanostructured dental materials and nanometric relationship between
dental materials and dental tissues.
Laboratory: Research Laboratory of the Dental Clinic, Dental Clinic, University Department of
Medical, Surgical and Health Sciences, Piazza Ospedale 1
Supervisor: Prof. Daniele Angerame
Nanotechnology is one of the most recent and innovative field of study in dental research (1). In
particular, the development of nanostructured dental materials and the study of the nanostructure of
already available dental materials have started promising lines of investigation.
In dentistry, composite resins constituted by inorganic fillers (e.g. glass, quartz or ceramic particles)
embedded in an organic resin matrix consisting of a mixture of dimethacrylates, are used for tooth
restoration. Composite resins were introduced in dentistry during the Nineteen Sixties and
underwent a substantive evolution during the following decades. One of their major changes
concerns the reduction of the filler particle size, so that nanofilled composites, with a filler size
ranging from 5 to 100 nm, have been nowadays developed and introduced in the dental practice
(2,3). Some authors advocate their use because of improved aesthetical and mechanical properties
compared to conventional packable composites (2,4,5). The nanofilled composites category is
heterogeneous and filler particle size is not the only significant factor in determining the material’s
mechanical performance (2). The resistance to surface wear and degradation plays a key role in the
success of restorative dentistry, which aims to avoid recurrent caries and other complications. For
these reasons, the identification of the best materials among those available on the market has
relevant clinical implications.
In implant dentistry the modification of the implant surface to promote the osseointegration
represents a remarkable matter of study and debate. There is general agreement that micro-rough
implants are more effective than smooth ones in terms of bone-to-implant contact rate and retention
into the bone (6); clinical data confirm this trend (7). Surface topography modifications at a nanoscale level constitute a recent frontier and the information about their possible benefits and
limitations appears to be still insufficient. Consequently, this issue needs further laboratory and
clinical investigations.
A very recent article about the physicochemical properties of nanomodified mineral trioxide
aggregate reports reduced setting time and increased microhardness even at lower pH values after
hydration (8), thus opening new research possibilities also in endodontics.
1.Ozak ST, Ozkan P. Nanotechnology and dentistry. Eur J Dent 2013; 7: 145-51.
2.Papadogiannis DY, Lakes RS, Papadogiannis Y, et al. The effect of temperature on the
viscoelastic properties of nano-hybrid composites. Dent Mater 2008; 24: 257-66.
118
3.Moszner N, Klapdohr S. Nanotechnology for dental composites. Int J Nanotechnol 2004; 1: 13056.
4.Mitra SB, Wu D, Holmes BN. An application of nanotechnology in advanced dental materials. J
Am Dent Assoc 2003; 134: 1382-90.
5.Beun S, Glorieux T, Devaux J, et al. Characterization of nanofilled compared to universal and
microfilled composites. Dent Mater 2007; 23: 51-9.
6.Shalabi MM, Gortemaker A, Van't Hof MA, et al. Implant surface roughness and bone healing: a
systematic review. J Dent Res 2006; 85: 496-500.
7.Balshe AA, Assad DA, Eckert SE, et al. A retrospective study of the survival of smooth- and
rough-surface dental implants. Int J Oral Maxillofac Implants 2009; 24: 1113-8.
8.Saghiri MA, Asgar K, Lotfi M, Garcia-Godoy F. Nanomodification of mineral trioxide aggregate
for enhanced physiochemical properties. Int Endod J 2012; 45: 979-88.
The research activity will be focused on the characterization of nanostructured dental materials by
direct laboratory and/or clinical research: (i) to study the surface characteristics and degradation of
dental restorative materials, such as nanofilled and/or nanohybrid composite resins and ceramics;
(ii) to define the clinical implications of the use of dental implants with nanostructured surface
topography.
A further objective might be the nanometric analysis of dental materials, e.g. mineral trioxide
aggregate or its nanomodified counterpart, under particular experimental condition, such as in case
of pH variations.
The first year will be mainly devoted to deepen the knowledge in nanostructured dental materials
and surfaces, with particular attention to restorative, prosthetic and implant dentistry. Ongoing in
vitro and in vivo experimentations about the surface degradation of a nanohybrid flowable
composite resin will be monitored and preliminary data will be gathered.
The possibility to apply systematic reviews and meta-analyses to the field of dental nanotechnology
will also be taken into account.
Research project
The surface deterioration of composite resins will be evaluated by profilometric analysis and
scanning electron microscope observation of positive resin replicas of specimens either aged in
vitro or under clinical conditions (i.e. after being applied to the tooth surfaces of volunteer subjects).
The use of other instruments (transmission electron microscope, small-angle X-ray scattering, and
atomic force microscope) to evaluate further characteristics of nanostructured dental materials or
conventional materials at a nano-scale level will be discussed with the Supervisor and, if needed,
included in the research project.
The Gantt chart is given as attachment.
During the first year I am going to perform extensive study of the literature concerning nanostructured dental materials in the fields of restorative, prosthetic, implant dentistry and
endodontology.
119
Furthermore, I have been to/I plan to attend the following national and international
meetings/courses on dental materials and related topics:
1.Metodologia della ricerca e preparazione di un lavoro scientifico in ortodonzia: corso
intensivo teorico-pratico, Florence, 28th and 29th January
2.17° Congresso Nazionale della Società Italiana di Odontoiatria Conservatrice, Rome, 15th17th February
3.Corso di alta formazione – La letteratura in ambito di ricerca scientifica: come leggerla,
interpretarla, selezionarla e proporla, Due Carrare (PD), 2nd March
4.20° Congresso Nazionale dei Docenti di Odontoiatria, Rome, 18th-20th April
5.46th Meeting of the Continental European Division of the International Association for Dental
Research with the Scandinavian Division, Florence, 4th-7th September
6.16th European Society of Endodontology Biennial Congress, Lisbon, 12th-14th September
7.32° Congresso Nazionale della Società Italiana di Endodonzia, Bologna, 8th-10th November
120
FABIO DEL BEN
Title of the thesis: A bridge between the electrical and mechanical signature of cancer.
Laboratory: Nanomedicine - Udine
Supervisor: Giacinto Scoles (Uniud)
Tutors (if any): Scaini Denis (Units)
The field of bioelectrical signaling is focused on the link between cell biology and electrical events.
One of the main values of bioelectrical signals the electrical potential difference across plasma
membranes (Vmem)1. Vmem is the result of the complex activity of a lot of different trans-membrane
ion channels (Na+, K+, Cl-) and its values change from cell to cell, maintaining a general pattern of
high values in non-proliferating cells (e.g.: muscle and neurons) and low values in highproliferating, stem cells and cancer cells; it also displays fluctuations through the cell cycle. There
is evidence that changes in Vmem value can affect cell proliferation and differentiation1,2. It’s worth
noting that the biological effect is not linked to the particular ion channel affected, but to the final
change in Vmem value1.
Nowadays methods applied to change Vmem are modulation of ion channels through different
approaches (pharmacological, knockout genes, siRNA) and patch-clamp. Since there are a lot of
feedback loops between ion channels and cell functions, we don’t know precisely the Vmem value
obtained by inhibiting a channel; furthermore, we don’t know if the channel is part of a signaling
cascade: we could disrupt important pathways knocking it out. Besides this, ion channel expression
and Vmem are not necessarily linked: there can be the same Vmem value in different patterns of ion
channels, and there are different Vmem with the same expression of channels. Patch-clamp approach
is a single-cell technique, challenging and most likely leaving the cell damaged and dying in few
hours.
For these reasons we think that the best way for our purposes is to modify directly Vmem in a cell
population, without modifying ion channel expression, keeping the cells viable long-term: a recent
solution is using micro-electrode arrays (MEAs) coupled to nanostructures allowing for direct
intracellular electrical stimulation. Nanostructured that gave the best results are gold mushroomshaped microelectrodes (gMµEs) or vertical nanowire electrode arrays (VNEA). In particular,
gMµEs functionalized with RGD-based peptide provide multisite, simultaneous, intracellular
recording and stimulation for periods of (at least) days without damaging the cells3.
We know that cancer cells are softer than normal cells4, and our aim is to assess whether the
electrical and mechanical properties of cell membranes are linked, using atomic-force microscope
(AFM) to monitor the stiffness while we keep Vmem value close to the healthy one. If the membrane
stiffness can be reverted to normal values, we want to evaluate if other malignant features can be
reverted as well with this technique (E.g.: morphology, proliferation, gene expression).
121
1.
2.
3.
4.
Levin, M. & Stevenson, C.G. Regulation of cell behavior and tissue patterning by
bioelectrical signals: challenges and opportunities for biomedical engineering. Annual
review of biomedical engineering 14, 295-323 (2012).
Blackiston, D.J., McLaughlin, K.A. & Levin, M. Bioelectric controls of cell proliferation:
ion channels, membrane voltage and the cell cycle. Cell cycle 8, 3519-3528 (2009).
Spira, M.E. & Hai, A. Multi-electrode array technologies for neuroscience and cardiology.
Nature nanotechnology 8, 83-94 (2013).
Cross, S.E., Jin, Y.S., Rao, J. & Gimzewski, J.K. Nanomechanical analysis of cells from
cancer patients. Nature nanotechnology 2, 780-783 (2007).
-To establish a robust technique able to detect and control Vmem, using nanostructures and
MEAs.
-To define precise stimulation patterns able to:
oChange stiffness of cells
oArrest cancer cells at different cell cycle checkpoints
-Additional tasks (regenerative medicine)
oIncrease cell proliferation, especially of cells not normally cycling (neurons,
cardiomyocytes)
oA guide to stem cell differentiation.
-To establish the best method to stimulate the cells.
-To learn to use AFM
-To learn to use MEAs and stimulation/recording techniques
Research project
Activities:
-Isolation of cells from tumoral tissues and cell culture
-Electrophysiology
-AFM microscopy
-Optical/fluorescence microscopy
-Cell cycle labeling methods (e.g.: FUCCI method)
-Gene expression (RT-PCR, immunostainings)
-Single cell analytical techniques
Tasks:
-To culture cells on MEAs with nanostructures
-To monitor and modulate Vmem
-To track cell stiffness in response to Vmem changes.
-To track cell cycle/proliferation in response to Vmem changes.
122
Linz, 15-18 Feb 2013 – XV Annual Winter Workshop, Advances in Single-Molecule Research for
Biology and Nanoscience (12-14 Feb 2013 – Hands on Winter school)
Cycle of lectures on intermolecular forces held by prof. Giacinto Scoles.
Experiences in other labs.
123
ISMAEL ROMERO OCAÑA
Title of the thesis: “Nanomaterials for Solar Fuels”
Laboratory: “Department of Chemical and Pharmaceutical Sciences”
Supervisor: Prof. Paolo Fornasiero
Tutors (if any): Dr. T.Montini , Dr. J.J. Delgado
It is unquestionable that one of the most important challenges of our society is the development of
new energy strategies to tackle global warming and the exhaustion of fossil fuels. Therefore, the
design of innovative low-cost and environmentally friendly energy storage and conversion systems
is crucial for stable economic growth in a world whose energy needs are continuously increasing. In
this context, catalysis has been proven as a critical enabling science for developing the use of
alternative feedstocks, such as biomass or hydrogen, and increasing energy production efficiency.
Sunlight is one of the most abundant renewable and clean energy sources in the world. For this
reason, the research community has intensely studied how to use it for developing sustainable
energy production technologies. Unfortunately, conventional photovoltaic cells have the
inconvenience of their high production costs and daylight-dependency. In this context,
Photocatalytic fuel production has recently emerged as a promising approach to face the sustainable
energy production and more importantly its storage. Since the discovery of photocatalytic splitting
of water to produce hydrogen by Fujishima and Honda in 1972, [1] the approach have been
extended to the bio-alcohols photo-reforming and carbon dioxide reduction to carbon fuels. The last
option, so called synthetic photosynthesis, has the advantage of CO2 carbon capture and
sequestration. In addition to the production of carbon-neutral fuels, they can be directly used in
actual energy production systems in the residential, industrial and transportation sectors.
It should be pointed out that although the efficiency of water splitting and CO2 photoreduction
using solar radiation is poor, it is one of the most challenging tasks of environmental catalysis and
the US Department of Energy (DOE) has identified it as one of their priority research directions.
The DOE have suggested that efficiency higher than 10% should be reach to guarantee the
successful implementation of photocatalysis devices. At the present time, the most active
photocatalyst show a very poor efficiency of 1-5%. In this sense, traditional photocatlysts, such as
TiO2, commonly requires Ultraviolet light, so using only around 4% of the sunlight. Therefore, it is
desirable to develop active catalysts that harvest a wide spectrum of the solar light irradiation,
improving the catalyst efficiency.
The main aim of this project is to design new plasmonic TiO2-based photocatalysts for harvesting
visible light, which covers 43% of the sunlight. In this sense, it is well-known that noble metal
nanoparticles (Au, Ag, Pt, etc) can strongly absorb visible light [2] due to their unique Surface
Plasmon Resonance (SPR) properties. These noble-metal nanoparticles are able to transfer
124
photogenerated electrons to a metal-semiconductor and promote the generation rate of e-/h+ pairs in
the semicoductor. This configuration is obviously a promising photocatalyst that can efficiently
operate under visible light. The optical properties of some metal nanoparticles, such as Au and Ag,
(AuNPs) are dominated by Surface Plasmon Resonance (SPR) effects, which can be defined as the
collective oscillation of the conduction electrons induced by light
irradiation [3,4]. The synergy effect of the metal NP-semiconductor
is illustrated in figure 1 for the Ag/AgBr photo-catalyst.
Figure 1. The synergy effect of metal NP-semiconductors [adapted from 8]
The main objective of this project is the evaluation of the effect of different Au and Ag nanoparticle
architectures on the absorption properties of a number of TiO2 semiconductors with different
morphology (core-shells, cubes, rods, pyramids, etc.) and sizes. In the case of the TiO2
semiconductors I would pay especial attention to bi-dimensional nanoparticles to decrease the e-/h+
recombination rate.
Another of the central goals of this project is the development of supra-structured systems where
the most active metal-semiconductor configurations will be immobilized. This step is crucial before
real application. The work will be focused on the use of carbonaceous materials due to their unique
and controllable morphology and structural-electrical properties [9]
First year
9Synthesis of morphologically controlled TiO2 nanoparticles, paying special attention to one
and two dimensional nanoparticles with high crystallinity degree to reduce e-/h+
recombination. I will take advance of my knowledge in the synthesis of nanostructured
catalysts by solvothermal methods to successfully achieve this task.
9Synthesis of morphologically controlled Ag, Au and Ag-Au nanoparticles with enhanced
SPR. I will pay special attention to those system previously reported in the literature such as
metal-dielectric-metal (MDM) structures and double concentric nanoshells (DCNs).
9Sub-nanometric characterization of nanoparticles by mean of Advanced Electron Microscopy
Techniques, such as SEM, HRTEM, HAADF-STEM using associated XEDS and EELS
detector for chemical analysis.
Research project. The methodology proposed for achieving the previously describe reach objective
are based on the following four pillars:
125
1) Synthesis of novel nanomaterials, including the preparation of morphologically controlled
nanoparticles and hierarchically ordered nanomaterials taking advanced of the SPR properties of
noble metal nanoparticles.
2) Catalytic evaluation of water splitting and CO2 reduction under realistic conditions to evaluate
the deactivation process and the effect of the reaction parameter in both activity and selectivity.
3) Advanced characterization of the catalysts, preferentially, under operation conditions using TEM,
IR and light absorption techniques. Structural, morphological and compositional study of the newly
developed materials at subnanometric scale will be studied by both High Resolution Transmission
(HRTEM) and Scanning Transmission (STEM) Electron Microscopy. A number of analytical
techniques, such as XEDS and EELS, will be used to provide information about the composition
and electronic structure of the samples, including the plasmon properties of the metal nanoparticles.
Importantly, these techniques will allow us to determine the interactions between all the elements of
the complex multicomponent photo-catalysts. In addition, a model of the nanoparticles will be
obtained by combining experimental STEM-HAADF and HRTEM images with nano-structural
modeling and images simulation techniques. This model will be useful for theoretical calculations.
4) Use of advanced theoretical and computational methods to fully understand the catalytic
behavior and propose new formulations. This will be carried out in collaboration with other
international lab. In particular, the intensified electric field at the interface between the metal
particles and the subdomain on TiO2 would be studied by Finite Element Method (FEM)
electromagnetic simulation.
My research plan has the aim of solving real problem existing in the implementation of
Photocatalysis for sustainable energy production. For this reason, patenting results will be a
priority. Therefore, research results will only be published after an initial review of their potential
for patenting and commercial exploitation by the inventors, the local patent and technology units
and eventual industrial partners. In order to reinforce this objective, I will be trained in knowledge
transfer skills, which would facilitate the identification of patentable results. Presentation of oral
and poster communications in conferences, and publication of scientific papers in high impact
journals will also be one of my priorities
Sub-nanometric characterization of nanoparticles by mean of Advanced Electron Microscopy
Techniques, such as SEM, HRTEM, HAADF-STEM using associated XEDS and EELS detector for
chemical analysis will carry out at the University of Cadiz (Spain) sunder the supervision of Dr.
J.J. Delgado at the “Structure and Chemistry of Nanomaterials” group. Plasmon characterization
of metal NPs will be carried out by EELS technique. Activities of the PhD School. Conferences.
Clasification
126
1.- B1; B3; B4; F1; F3; F4; F15; F16; F17
2.- F (Energy& Environment)
References
1. A. Fujishima and K. Honda, Nature, 1972, 238, 37-38.
2. Peng Wang, Baibiao Huang, Ying Dai and Myung-Hwan Whangbo, Phys. Chem. Chem. Phys., 2012, 14,
9813-9825.
3. A. Merlen, V. Gadenne, J. Romann, V. Chevallier, L. Patrone and J. C. Valmalette, Nanotechnology,
2009, 20, 1-7.
4. D. J. Wu, X. D. Xu, X. Liu, J. Solid State Commun. 148, 163-167, 2008.
5. P. Wang, B. B. Huang, X. Y. Qin, X. Y. Zhang, Y. Dai, J. Y. Wei and M.-H. Whangbo, Angew. Chem.
Int. Ed., 2008, 47, 7931–7933.
6. H. Zhang, X. F. Fan, X. Quan, S. Chen and H. T. Yu, Environ. Sci. Technol., 2011, 45, 5731–5736.
7. W. Liu, W. B. Hou, P. Pavaskar, M. Aykol and S. B. Cronin, Nano Lett., 2011, 11, 1111–1116.
8. Jing Jiang, H. Li and L. Zhang, Chemistry A European Journal, 2012, 18(20), 6360–6369.
9. R. Leary and A. Westwood, Carbon, 2011, 49, 741-772.
127

Graduate student LUCA OMICIUOLO
Title of the thesis: Study of the growth processes and of the physical and chemical properties of
graphene-based low dimensional systems
Laboratory: Surface Science Laboratory (Physics Department – University of Trieste, Elettra
Sincrotrone Trieste) and SuperESCA beamline at Elettra.
Supervisor: Prof. Alessandro Baraldi
Co-supervisor: Dr. Silvano Lizzit
•
Since its first isolation in 2004 [K.S. Novoselov et al., Science 306, 666 (2004)] graphene, a
single layer of carbon atoms arranged in a honeycomb lattice, has been the subject of a number of
scientific publications. This staggering interest is motivated by the outstanding properties of this
material. In particular, the valence and conduction bands of graphene show a unique conical shape
at the high-symmetry points of the first Brillouin zone, leading to a linear E(k) energy dispersion
for electrons near the Fermi energy [K.S. Novoselov et al., Nature 438, 197 (2005)]. This linear
dispersion is qualitatively identical to that of ideal massless fermions and is responsible for a series
of unusual electronic transport properties, making graphene extremely interesting for application in
nano-electronic devices [A.K. Geim et al., Nature Materials 6, 183 (2007)]. This is why reliable
methods for large-scale, high-quality graphene synthesis are needed.
A promising way for mass production of high quality carbon layers is the epitaxial growth of
graphene on transition metal surfaces [X. Li et al., Science 324, 1312 (2009)]. The problem, in this
case, is that graphene grown on metal surfaces generally interacts strongly with the substrate, so
that most of its peculiar properties are lost [A.B. Preobrajenski et al., Phys. Rev. B 78, 073401
(2008)]. One way to overcome this drawback is to remove the substrate by chemical etching, once
the layer is formed, but this method poses a series of difficulties, and leads to low quality graphene
layers due to the high concentration of point and extended defects, such as vacancies, grain
boundaries and multiple domains.
Figure 1: Synthesis of SiO2 under epitaxial graphene on Ru(0001). Each process step is schematically
illustrated. [S. Lizzit et al., Nano Lett. 12, 9 (2012)]
A valid alternative to this procedure consists in the epitaxial growth of graphene on metal
surfaces, and subsequent oxidation of the substrate below the carbon layer. As a matter of fact, a
number of studies have shown the possibility to intercalate various atomic species underneath
graphene layers. In the recent years, our research group has developed a new method to synthesize
128
graphene on SiO2 [S. Lizzit et al., Nano Lett. 12, 9 (2012), see also the comments on Research
Highlights, Nature Nanotech 7, 613 (2012)]. After intercalation of Si atoms under a well-ordered
graphene layer grown on ruthenium substrate, the Silica layer below graphene was obtained by
direct oxidation of the Si interface.
The development of new efficient procedures to grow high quality, large-scale, graphene
layers directly on dielectric material with tunable interaction, would be extremely useful for
fundamental studies on quasi free-standing graphene, and it would a primary requirement for
fabrication of graphene based nano-electronic devices.
The main objectives of my PhD research program are the comprehension of the physical and
chemical properties of epitaxial graphene grown on different monometallic and bimetallic surfaces,
and the development of techniques to decouple it from the substrate, by means of atomic
intercalation and/or subsequent selective oxidation. Beside that I will also be involved in the other
research activities carried out in our laboratory, with the goal to obtain graphene functionalization
by means of metal nanoclusters deposition, using a mass-selected nanoclusters source which is
currently under commissioning. Here is a list of the main issues I'm going to deal with:
. structural and electronic characterization of epitaxially grown graphene on transition
metals and bi-metallic surface alloys;
. carbon layers decoupling from metal substrates by intercalation of various atomic species,
or by direct substrate oxidation;
. graphene functionalization by means of mass-selected nanoclusters ;
. investigation of the structural, electronic and chemical properties of nanoclusters supported
on epitaxial graphene.
During the first year of my PhD I'm going perform a part of the activities listed above, in
particular:
. characterization of epitaxial graphene growth on the Ni3Al(111) bi-metallic alloy surface
and selective Al oxidation by intercalation of oxygen. The GR/Al2O3/Ni3Al properties will be
investigated by means of diffraction techniques (LEED), photoelectron spectroscopy (XPS)
and angle-resolved photoelectron spectroscopy (ARPES).
. oxidation of transition and noble metal clusters supported on epitaxial graphene.
Research project
129
As already anticipated in the previous paragraphs, my work will be aimed to two main issues:
epitaxial growth of graphene on transition metal and bi-metallic alloy surfaces, and selective
oxidation of the substrate below the carbon layer.
More precisely, due to their heavy use in electronics, Fe, Si, Al, Zr and Ti oxides are very
interesting substrates, and it would be extremely useful to produce high quality graphene layers
grown on these oxides. Unfortunately it is not possible to grow graphene directly on oxide surfaces
by using chemical vapour deposition. One way to overcome this obstacle is to use, as a substrate,
transition metal surface alloys containing the mentioned atomic species, such as, for example, NiAl
and FeAl surface alloys. As a matter of fact, transition metal surfaces are known for their catalytic
properties for hydrocarbons dissociation, which can be exploited to grow extended high quality
carbon layers. Therefore, our goal will be to characterize graphene grown on such bi-metallic
surface alloys and then to selectively oxidize the atomic species at issue by means of oxygen
intercalation under the carbon layer.
Another strategy that will be followed to obtain graphene supported on oxide thin layers,
consists in intercalation of various atomic species underneath the carbon layer followed by selective
oxidation of these species.
Moreover I will join my laboratory group in the setup of a mass-selected nanoclusters source
which, once completed, will be a unique experimental apparatus in the national research landscape.
This machine will allow us to study physical and chemical properties of transition and noble metals
nanoclusters as a function of their dimensions.
Finally I will spend few months with the research group headed by Prof. Ph. Hofmann at the
Aarhus University in Denmark. This laboratory is somehow complementary to ours, being
specialized in valence band photoelectron spectroscopy, while both the Surface Science Laboratory
and the SuperESCA beamline are focused in core level photoelectron spectroscopy and
photoelectron diffraction.
To deeply investigate all these system I will rely on state-of-the-art experimental techniques,
in particular: X-ray Photoelectron Spectroscopy (XPS) and X-ray Photoelectron Diffraction (XPD),
both from conventional sources and from synchrotron radiation, Low Energy Electron Diffraction
(LEED) and Angle Resolved Photoelectron Spectroscopy (ARPES).
Beside that, our research group has a productive collaboration partnership with the group of
Prof. Dario Alfè at the University College of London, who will provide Density Functional Theory
(DFT) calculation to support our experimental work.
130
1st year
SCHEDULED ACTIVITIES
2nd year
3rd year
1st term 2nd term 1st term 2nd term 1st term 2nd term
Growth and properties of epitaxial graphene
Graphene on NiAl alloys
Graphene on Ti-based alloys
Graphene on FeAl alloys
Graphene supported nanoclusters
Chemical reactivity of transition metal nanoclusters supported on epitaxial graphene
Chemical reactivity of noble metal nanoclusters supported on graphene
•
During the first year I will attend schools, seminars and courses focused on increasing my
knowledge, both at the theoretical and experimental level, on topics related to my research
activities. Both ELETTRA and the IOM-CNR TASC Laboratory regularly hold seminars on this
topic, moreover several theoretical seminars on graphene and low-dimensional systems periodically
take place also at the ICTP and at SISSA and I'm going to attend them.
Lecture activities will be complemented by the reading of subject-specific papers and reviews,
to keep myself up to date in the fields related to my research project. Moreover I'm going to attend
several courses on data acquisition and analysis software, such as LabView, Igor Pro and Cad, held
at the Elettra synchrotron radiation laboratory.
Finally, during the period at the Aarhus University, I expect to attend some subject-specific
lectures on electronic structure in condensed matter.
131
LAERTE LUIGI PATERA
Title of the thesis: “An in-situ and in-operando study of graphene growth and properties on metal
surfaces”
Laboratory: IOM-CNR, TASC Laboratory
Supervisor: Dr. Cristina Africh
Write a description of the state of the art and the motivations for the research project, justifying
particularly all aspects related to nanotechnology. Please keep the description in one page
maximum, including graphs, literature and tables. Please do note modify characters and paragraphs
settings of the present document.
Graphene1, a single layer of graphite, has recently focalized the attention of physicists, chemists and
engineers for its electronic and structural properties2,3. Electrons in graphene obey a linear
dispersion relation and behave like Dirac massless relativistic particles, leading to peculiar
electronic properties such as the quantum Hall effect, ambipolar electric field effect, good optical
transparency. Due to its huge carrier mobilities (200.000 cm2/Vs), high thermal and electrical
conductivity, graphene based devices can potentially change semiconductor electronics for
nanoelectronics applications4 and in the gas sensors field5. One of the most promising techniques
for producing high-quality single layers of graphene is Chemical Vapour Deposition (CVD), using
hydrocarbons as C feedstock and the surfaces of transition metals as catalyst for its decomposition,
like Cu(111), Ru(0001), Ir(111), Ni(111), Pt(111), Co(0001) and Rh(111)6. At the moment, the
state of art to fabricate low cost / mass production suitable graphene is reached with catalytic
copper surfaces, because of the low C solubility and the small mismatch between graphene and
Cu(111) lattices (≈4%), that lead to large areas of high quality monolayer graphene7. However, the
high temperatures needed to dissociate hydrocarbons (≈1000°C), close to the melting point of
copper, cause a sublimation of the substrate and a surface faceting. For these reasons, other
substrates such as bimetallic alloys8 and nickel surfaces9 are been attracting attention because of the
possibility to grow graphene at lower temperature (400-600°C). Despite this, graphene uniformity
and layer control over large areas remain very difficult to achieve on this kind of substrates and a
clear comprehension of the growth mechanisms and of the influence of parameters as temperature,
hydrocarbon pressure and C concentration into the sample is missing at the moment. So, further
efforts to shed light on how to control graphene quality through the CVD parameters are needed.
In addition, the possibility to grow graphene with tunable chemical activity, through defects
engineering, is very promising for the heterogeneous catalysis field, but, a deeper understanding of
the correlation at the atomic scale between defects and chemical properties is still needed.
(1)
Geim, A.; Novoselov, K. Nature materials 2007, 183–191.
132
(2)
Chen, Z.; Lin, Y.; Rooks, M.; Avouris, P. Physica E: Low-dimensional … 2007, 40, 228–232.
(3)
Schedin, F.; Geim, A. K.; Morozov, S. V; Hill, E. W.; Blake, P.; Katsnelson, M. I.; Novoselov, K. S.
Nature materials 2007, 6, 652–5.
(4)
Schwierz, F. Nature nanotechnology 2010, 5, 487–496.
(5)
Hill, E. W.; Vijayaragahvan, A.; Novoselov, K.; Exfoliation, A. M. 2011, 11, 3161–3170.
(6)
Batzill, M. Surface Science Reports 2012, 67, 83–115.
(7)
Li, X.; Cai, W.; An, J.; Kim, S.; Nah, J.; Yang, D. Science 2009, 324, 1312–1314.
(8)
Weatherup, R.; Bayer, B.; Blume, R. Nano Letters 2011, 11, 4154–4160.
(9)
Kim, K.; Zhao, Y.; Jang, H.; Lee, S.; Kim, J. Nature 2009, 1–5.
Write a description of the objectives to be reached in three years. Few lines and a bulleted list is
sufficient.
I am going to study graphene growth and properties on metal surfaces. Starting from model systems
(single-crystal substrates, UHV), I intend to achieve a comprehension also in conditions typical of
the industrial reactors for graphene and carbon nanotube CVD (poly-crystalline catalysts, higher
hydrocarbons pressure), aiming to bridge both material and pressure gaps. In addition I will
characterize different kinds of graphene grown on metal substrates, particularly focusing on
electronic properties and chemical activity.
The main steps are:
•First year: study of graphene growth dynamics on single crystal surfaces by CVD, during
exposure at low pressures of hydrocarbons (10-7 mbar range) and characterization of the
graphene properties, like electronic structure and chemical properties.
•Second year: extension of the understanding reached with model system to “real” conditions:
graphene growth on poly-crystal surfaces.
•Third year: investigation of graphene growth at higher hydrocarbon pressure (10-5-10-3 mbar
range).
Write a description of the objectives to be reached in the first year. Few lines and a bulleted list is
sufficient.
During this first year I will focus on the study of graphene growth on Ni substrates, starting from a
model system such as a Ni(111) single crystal surface, trying to reach a control on graphene
structural quality, through different growth mechanisms and CVD parameters.
133
•Study of the correlation between growth parameters (temperature, hydrocarbon pressure, level
of C contamination into the sample), growth mechanisms and graphene quality (defect
density and structure, domain size).
•Identification of reproducible recipes to obtain different kinds of graphene on Ni(111), by
changing the CVD parameters, and characterization of the electronic and structural
properties of the grown surfaces.
•Investigation of the chemical properties of CVD graphene via controlled formation of defects
and surface morphologies.
Research project
Write a description of the research program divided into activities and tasks, describing also the
tools to be used. Include also a project Gantt diagram
During my PhD, I aim at investigating graphene on metallic substrates in different CVD conditions,
focusing on growth dynamics and electronic structure, and characterizing the physical/chemical
properties induced by defects in the honeycomb lattice. The main steps are:
1.to follow the graphene growth by CVD under in-situ and in-operando conditions on metal
surfaces, both for model systems (well oriented single crystals) and more “realistic”
substrates (thin poly-crystalline films).
2.to study of the correlation between growth parameters, growth mechanisms and graphene
quality.
3.to achieve recipes to obtain different kinds of graphene, changing the CVD parameters.
4.to understand how to control particular defect structures formation and to exploit their
chemical properties, with particular attention to possible applications in heterogeneous
catalysis.
5.to extend the expertise reached for low pressure CVD (10-7 mbar range) to higher pressure
conditions (10-5-10-3 mbar range) trying to bridge the so-called “pressure gap”.
In order to reach these goals, I will use some of the standard surface science techniques to
investigate under in-situ and in-operando conditions the graphene formation, structures and
properties. The main instrument that I will use during the PhD is a Variable Temperature Scanning
Tunneling Microscope (VT-STM), which allows us to follow surface dynamics at high temperature
and during gas exposure with atomic resolution. In addition, also electronic spectroscopies will be
used, allowing for complementary information, especially X-ray Photoelectron Spectroscopy
(XPS), Ultraviolet Photoelectron Spectroscopy (UPS) and Angle Resolved Photo-Electron
Spectroscopy (ARPES).
The activities that will be performed are the following:
134
1.Real time in-situ and in-operando measurements of graphene formation on Ni substrates
during hydrocarbon exposure with both STM and XPS.
2.Characterization of the as-grown graphene morphology at the sub-nanometer scale with STM.
3.Photoelectron spectroscopy measurements to investigate the electronic band structures of the
graphene layers grown by CVD.
4.High pressure CVD growth studies with in-situ and in-operando XPS measurements
(available at “Bessy” synchrotron) and post-growth STM data.
5.STM characterization of the different defects morphologies on CVD-graphene.
6.STM and XPS investigations of the interactions between these defective structures and
different adsorbates (e.g. CO, CO2, O2, water, etc…).
7.Creation of recipes to obtain graphene layers with a specific chemical activity.
Write a proposal of the educational activity to be carried out during the first year.
During the first year I will attend a LabView course. The motivation is that this software integrates
a lot of tools that engineers and scientists need to build a wide range of applications like data
acquisition and instrument control.
I will also take part to several seminars, e.g. the ones organized at Elettra, at TASC laboratory and
at the University , concerning topics useful to my project.
135
ELENA PELLIZZONI
Title of the thesis: Interatomics in tumor cells studied with a nanotechnology approach
Laboratory: 330-333, dept. of chemical and pharmaceutical science
Supervisor: Giuseppe Toffoli
Tutors (if any): Federico Berti, Flavio Rizzolio
The aim of this project is the identification, preparation and characterization of artificial or
biological binder molecules to be used as sensing elements for the development of innovative
nanobiosensors to apply in cancer therapies. The target are three anticancer drugs: paclitaxel 1a,
irinotecan 2a and sunitinib 3a.
3b: R = CH2CH 2CH 2CH2NEt2 , R' =
N N
N
4
3c: R' = H, R = -(CH2 )5NH 2
O
Scheme 1
The narrow therapeutic indices and the inter-individual pharmacokinetic variability of anticancer
drugs, cause many sides effects leading poor patient compliance and decreasing the efficiency of
the therapy. In this frame, Therapeutic Drug Monitoring (TDM) may represent a valuable
tool for the clinician to quantify drug level in biological fluids during the therapy and verify the
drug effectiveness and the tolerability of the therapy. Therefore TDM is useful to establish an
individual dosing regimen of drug [Alnaim,2007]. High-performance liquid chromatography or gaschromatography are the analytical methods usually implemented for determining drug plasma
136
concentrations [Schoemaker, 2003], however these methods are laborious and not always practical
for routine implementation [De Jonge, 2005].
So the ultimate goal of this project is the development of biosensors for irinotecan, paclitaxel and
sunitinib
that will be used for the design of new point of care devices or test kits useful for
clinicians or patients to quantify blood drug levels.
Artificial receptors /binders with high specificity for the target molecules are useful to this purpose.
Such
binders interact with the target through the molecular recognition mechanisms that play a
key role in biology, where enzymes, antibodies and drug receptors catalyze biochemical
reactions, neutralize
exogenous antigens and bind agonists and antagonists. The high specificity and catalytic activity of
these
macromolecules are due to binding sites in which multiple interactions can be established with the
target
molecule [Pavan, 2011].
Binder elements from biological sources (such as antibodies), or
developed by artificial, bio-inspired design (such as designed peptides [Hong, 2012] and imprinted
polymeric materials), are widely used in drug delivery, drug targeting, sensing and diagnostic field.
For example, antibody-based immunoassays have been developed for the detection of paclitaxel in
Taxus tissues and in human serum during clinical trials [Grothaus, 1995; Svojanovsky, 1999].
Anti-irinotecan and anti-SN-38 antibodies have been exploited in the development of ELISA assays
[Saita, 2000].
Molecularly imprinted polymers, instead, are micro- and nano-structured materials with three
dimensional cavities that complements the shape, structure and functional groups of the target
molecule.
Molecularly imprinted polymers are characterized by long-term stability and by the ability to
perform well under harsh conditions, such as high temperature, extreme pH values and organic
solvents. Therefore MIPs have found much interest in the production of sensing elements, of
stationary phases for chromatographic separations and affinity solid-phase extraction, of polymeric
nanoparticles for drug delivery, and of catalysts by enzyme mimicking approaches [Flavin, 2008].
Finally, electrochemical sensors with MIP are very useful for the production of point of care
devices [Sharma, 2012].
References
Alnaim L.(2007) Therapeutic drug monitoring of cancer chemotherapy. J Oncol Pharm Practice 13: 207-221
137
De Jonge M.E., Huitema A.D.R., Schellens J.H.M., Rodenhuis S., Beijnen J.H.(2005)Individualized cancer chemotherapy. Strategies
and performance of prospective studies on therapeutic drug monitoring with dose adapttion. Clin.Pharmacokinet. 44(2):147-173
Flavin K., Resmini M.(2009) Imprinted nanomaterials: a new class of synthetic receptors. Anal Bioanal Chem 393: 437-444
Grothaus P.G., Bignami G.S., O’Malley S., Harada K.E., Byrnes J.B., Waller D.F., Raybould T.J.G.(1995) Taxane-specific
monoclonal antibodies: measurement of taxol, baccatin III, and”total toxanes” in taxus brevifolia extracts by enzyme immunoassay.
Journal of natural products. 58(7): 1003-1014
Hong Enriquez R., Pavan S., Benedetti F., Tossi A., Savoini A., Berti F., Laio A.(2012) Designing short peptides with high affinity
for organic molecules: a combined docking, molecular dynamics and Monte Carlo approach. J. Chem. Theory Comput. DOI:
10.1021/ct200873y
Pavan S., Berti F.(2011) Short peptides as biosensor transducers. Anal Bioanal Chem 402(10): 3055-70
Saita T., FujitoH.,Mori M.(2000) Development of ELISAs for irinotecan and its active metabolite SN-38. Biol.Pharm.Bull. 23(8):
911-916
Schoemaker N., Rosing H., Jansen S., Schellens J., Beijnen J.H. (2003) High-Performance Liquid Chromatographic analysis of the
anticancer drug irinotecan (CPT-11) and its active metabolite SN-38 in human plasma. Therapeutic drug monitoring. 25:120-124
Sharma P.S., D’Souza F., Kutner W.(2012) Molecular imprinting for selective chemical sensing of hazardous compounds and drugs
of abuse. Trends of Anal Chem 34: 59-77
Svojanovsky S.R., Egodage K.L., Wu J., Slavik M., Wilson G.S. (1999) High sensitivity ELISA determination of taxol in various
human biological fluids. J. Pharm. Biomed. Anal. 20: 549-555
•Chemical modification of target molecules for either immobilization on gold or polymeric
surfaces, bioconjugates synthesis, and reference compounds labeling, in order to develop
both MIPs and antibody based technologies.
•Synthesis of molecularly imprinted polymers with irinotecan, paclitaxel and sunitinib as
template
and study of MIPs binding capacity.
•Immunogenic bioconjugates will be also prepared with the modified drugs, for the production
of
antibodies and the preparation of drugs labelled with enzymes (HRP), for the development
of enzymatic assays.
•Setup and development of ELISA with the best antibodies.
•Development of nanosensors based on the artificial receptors.
•Irinotecan, paclitaxel and sunitinib will be chemically modified with linkers for
immobilization over solid surfaces for the screening of receptors libraries and for the
measure of binding affinity on standard biosensors like surface plasmon resonance (SPR).
•Different functional monomers will be chosen and synthetized or modified, for the synthesis
of molecularly imprinted polymers.
Research project
138
1) Paclitaxel, irinotecan and sunitinib chemistry
Paclitaxel will be acylated with succinic anidride to obtain the hemisuccinyl-derived 1b, however,
the carbonyl group at position 9 can also be modified to obtain the carboxymethyl oxime 1c
(scheme1).
Irinotecan will be modified on aromatic ring by diazotization to obtain the maleimide derivate
2b, while lactone ring opening leads a free carboxylic group that can be conjugated or modified in
the same way of paclitaxel. Finally the only one hydroxyl group of the drug will be also considered
in order to obtain the hemisuccinate 2c. Diazotization of the indole ring may be possible also on
sunitinib to obtain the maleimide derivate 3b. Moreover, the diethylaminoethyl chain can be
replaced with a chain containing a primary amine group, like in 3c, to allow direct conjugation with
biotin and proteins.
2) Synthesis of molecularly imprinted polymers
In this project MIPs with specific binding sites for irinotecan, sunitinib and paclitaxel will be
synthesized.
Small peptides or single amino-acids as functional monomers will be chosen. Short peptides will be
designed by computational studies with the collaboration of prof. Laio (SISSA) and will be
produced by automated solid phase peptide synthesis. The N-terminus of peptides or amino-acids
will be acryloylated with acryloyl chloride to allow the monomer incorporation into the polymeric
matrix of MIP.
Functional monomers will be characterized by nuclear magnetic resonance (NMR)
spectroscopy and
electrospray ionization (ESI)-mass spectrometry, while the conformation of peptides in solution will
be
investigated with circular dichroism. Binding affinity of peptides for the target molecule will be
studied
by fluorescence spectroscopy and isothermal calorimetry. The size of MIP nanoparticles will be
characterized by dynamic laser light scattering. Rebinding tests will be performed to assess
the MIP
capability to capture target molecules.
3) Development of antibodies
Modified drugs will be conjugated with cationized bovine serum albumin or keyhole limpet
hemocyanin
to obtain immunogens for the immunization of rabbits or mice, to raise polyclonal antisera or
monoclonal full chain antibodies. Phage libraries of single chain Fv antibodies will be also screened
for
anti-target globulins. The antibodies will be purified by affinity chromatography, and their binding
capability will be characterized by immunoassays and surface plasmon resonance analyses.
Horseradish peroxidase conjugates will act as enzyme-labelled references for the developing of
139
competitive ELISA assays, which will be validated on spiked and real samples in both serum and
plasma, by statistical comparison with standard liquid chromatography-mass spectrometry (LC-MS)
titrations.
4) Development of nano-sensors
Atomic force microscopy (AFM) will be employed to develop a novel approach to detect drugs and
metabolites able to quantify drug levels in a very small volume of plasma, blood or tissue. This
device will be based on a competitive assay between the drug linked on a gold surface and the free
drug in the sample for the binding to the antibody.
AFM technique will be also employed to test molecularly imprinted polymers because they acts as
receptors for a specific drug.
Finally the new developed sensors and ELISA assays will be tested to optimize the working
conditions,
and define the selectivity and sensitivity limits. Also matrix effects and the sensors applicability of
plasma samples will be evaluated.
Activity
month
Î
1
Irinotecan modifications
1
Sunitinib modifications
1
Paclitaxel modfications
2
Synthesis of monomers
2
Synthesis of MIPs
2
Characterization of MIPs
3
Immunization
3
Antibody selection
3
ELISAs setup
4
5
6
3
6
9
12
15
18
21
24
27
30
33
36
Nanosensors setup for
TDM
TDM in purified solutions
and cultured cells
TDM in complex samples
(serum-plasma-blood)
Courses:
•“Hybrid organic-inorganic nanoparticles” (Prof. Lucia Pasquato)
•“Experimental design and optimization” (Prof. Cynthia Ebert)
140
•“Introduzione alla sintesi organica” (Prof. Federico Berti)
Schools:
•Interdisciplinary Phd Spring School (7-8 March 2013)
141
DAVIDE PORRELLI
Title of the thesis: “Nanocomposites biomaterials based on polysaccharides and carbon
nanostructures for biomedical applications”
Laboratory: Department of Life Science, building Q, lab. 113
Supervisor: Prof. Sergio PAOLETTI
Tutors: Massimiliano BORGOGNA, Ivan DONATI
This project is aimed at designing innovative biomaterials composed by natural biopolymers
(alginate, hyaluronic acid, chitosan) and Carbon Nanostructures (CNSs) for biomedical
applications; in particular, these materials will be developed for the treatment of Spinal Cord Injury
(SCI) and for bone tissue regeneration.
Carbon Nanostructures (CNSs) have been receiving increasing attention during the last years for
their unique physical and chemical characteristics that made these structures good candidate for
their use for neurological and tissue engineering applications[Nano Lett. 2010, 10, 3223-30].
Although the current scientific data have shown conflicting results about potential nano-toxicity of
CNSs, many studies are pointing out the biocompatibility of several forms of CNSs (especially in
functionalized form) and their ability to support growth and proliferation of cells like neurons and
osteoblasts [J. Tiss. Eng. 2012, 2, 1, 674287; Chem. Neurosci. 2012, 3, 611-8]. The use of CNSs in
combination with bioactive biopolymers aims at the development of novel biocompatible
nanocomposites in which biopolymers are implemented by physical and biological properties of
CNSs [Life Sciences 2010, 87, 215-22]. The advancement of these materials with respect to the
state of the art is briefly tackled in the following paragraphs.
Bone tissue regeneration: possible limitations of the polymers currently used for bone regeneration
include lack of bioactivity and poor mechanical properties; an ideal scaffold for bone tissue
regeneration should be able to promote osteoblasts proliferation and migration, and should have
mechanical properties similar to that of bone. Moreover it should have a porous structure with
interconnected pores and controlled size in order to allow an excellent osteointegration [Ann. of
Biom. Eng. 2012, 40, 1628-40; Adv. Mater. 2012, 24, 4995-5013]. The use of CNSs in combination
with biopolymers could implement scaffold properties by modulating mechanical strength and
osteoinductivity [Int. J. Biol. Macromol. 2010, 46, 281-3].
Spinal Cord Injury treatment: SCI is a devastating clinical condition that significantly impacts the
ability of affected individuals to produce functional movements and often results in paraplegia or
quadriplegia [Nat. Rev. Neurosci. 2006, 7, 644-53]. Currently there are no therapies able to repair
the damage of the spinal cord; recent strategies have involved the study of a plethora of curative
interventions towards prevention of cell death, or towards stimulation of axonal re-growth,
142
enhancement of axonal transmission or amelioration of secondary damage [Nat. Rev. Neurosci.
2006, 7, 644-53; Spinal Cord 2005, 43, 134-61; Nat. Rev. Neurosci. 2006, 7, 628-43; Exp. Neurol.
2002, 174, 125-36; Exp. Neurol. 2012, 235, 100-9]. The use of bioactive biomaterials as synthetic
bridging implants able to transmit electrical signals for SCI treatment has not yet been transferred
into clinical trials: the ongoing SCI clinical trials continue to rely mainly on the pharmacological
approach, with the only few exceptions of the stem cell-based therapies, which, however, have not
provided so far convincing evidence of clinical efficacy. Applications of nanotechnology with
potential clinical impact include the use of nano-engineered functional scaffold systems for
promoting neural regeneration following both acute and chronic injury. These approaches attempt
to create chemical, physical and biological environments that support/promote the function or
regeneration of central axons. The polymeric materials employed for scaffold design provide a pure
biomimetic environment: thus, the regeneration process is not based on the implementation of
electrical signals. Hyaluronan-based materials have been tested in models of peripheral nerve and
SCIs. The presence of a high molecular weight form of hyaluronic acid has been linked to scar-free
wound healing, leading to a reduced glial scar response [Neurosci. Lett. 2012, 519, 103-14]. The
rationale behind incorporating CNSs into a novel injectable/implantable biomaterial is that of
overcoming the limits of conventional polymer-based scaffolds, such as the lack of electrical
conductivity: it has been proposed that one of the main issues in neural tissue regeneration is the
electrical conductivity of the biomaterial which would favor neural transmission and can improve
mechanical and rheological properties of ECM-like structures [Nat. Nanotech. 2011, 6, 13-22].
CNSs moreover can affect cell behavior and promote attachment, growth, differentiation and long
term survival of neurons [ACS Chem. Neurosci. 2012, 3, 611-18].
The main objective of the project is the preparation of biocompatible nanocomposites in the form of
scaffolds or injectable matrices based on natural-based biopolymers (such as alginate, hyaluronic
acid, chitosan and derivatives) and CNSs. This goal will be pursued through the following
intermediate objectives:
• optimization of the preparation of the nanocomposite systems; • physical‐chemical and mechanical characterization; • evaluation of in vitro biocompatibility and bioactivity. In case of promising in vitro results, in vivo tests will be considered to test the materials on suitable animal models. Objectives for the first year:
143
• literature survey on the most suitable components and solutions for the applications targeted by this project; • selection of the components among various polysaccharides and CNSs; • preparation and characterization of homogenous solutions and chemical/physical and rheological characterization; • preparation and characterization of homogenous hydrogels and scaffolds with desired chemical and physical features. Research project
The research project foresees the production of different nanocomposites materials in form of
injectable fillers or 3D scaffolds composed of a polysaccharide matrix and CNSs. Morphological,
mechanical and physico-chemical characterizations will be performed. In addition, the effects on
eukaryotic cells will be studied in vitro and a final in vivo study will be considered in case of
encouraging in vitro results.
The activities and tasks of this project are listed below:
1.Materials selection and analysis: • analysis and evaluation of physical‐chemical properties of the selected biopolymers (Viscosimetry, Nuclear Magnetic Resonance); • analysis of functionalized CNSs (TGA, SEM, TEM). 2.Design and characterization of the nanocomposite formulations: • preparation of the nanocomposite formulations; • characterization of the materials in the form of solutions, hydrogels and freeze‐dried scaffolds (rheological tests, mechanical tests, SEM, TEM, µ‐CT) 3.In vitro biological characterization: • biocompatibility and cytotoxicity assays of the materials (LDH, MTT); • evaluation of the material ability to promote axonal regeneration and synaptogenesis; • evaluation of the material ability to stimulate osteoblasts activity and inhibition of osteoclasts activity (Alamar Blue, Alizarin Red assays, Confocal Microscopy, SEM); 1st year
2nd year
3rd year
144
Materials selection and analysis
Design and characterization of the nanocomposite formulations Biological characterization Educational Activity foreseen
University courses:
• Biomaterials, artificial organs and prostheses (prof. Sbaizero Orfeo; Master Degree in Process
and Materials Engineering, 6 CFU)
• Introduction to organic spectroscopy (prof. Felluga Fulvia; Bachelor Degree in Chemistry, 4
CFU)
I will attend laboratory activities to improve my knowledge on:
• in vitro and in vivo tests of biomaterials and nanocomposites; • Physical/chemical and mechanical characterization of nanocomposites; • preparation and characterization of nanostructured materials. I will attend the Interdisciplinary PhD Spring School (Udine, March 7 – 8, 2013) and other school and seminars. Scuola di dottorato di ricerca in Nanotecnologie: attività 2013
145
CANDIDATE PASQUALE SACCO
Title of the thesis: Novel biomaterials for innovative therapies in the severe wounds treatment
Laboratory: Department of Life Science, Ed. Q, First Floor, Room 105
Tutors: Dr.ssa Eleonora MARSICH, Dr. Massimiliano BORGOGNA
Nonhealing wounds (such us diabetic and pressure ulcers) are wounds which have not undergone
the normal process of healing because the three phases of healing (the inflammatory, proliferative
and remodeling phases) have been prolonged or did not progress in an orderly fashion.
Non-healing skin lesions are a fertile ground for bacteria proliferation that contribute to make the
lesion worse, causing a massive and persistent inflammatory response [5]. Proper treatments of
wounds require the employment of pro-regenerative and anti-inflammatory factors associated to
antimicrobial agents such as antibiotics and antiseptics [6]. However, antibiotics topically applied
often lead to the development of bacterial resistance and sensitization. In this direction, employment
of alternative wide-spectrum antibacterial agents is preferred to antibiotics. Several metals including
Silver (Ag) have shown efficacy for the treatment of infections by inhibiting Gram+ and Gramgrowth at very low concentrations [7]. Silver nitrate solutions [8] and silver sulfadiazine [9] are
commonly used in the treatment of burns. Both of these solutions have drawbacks: Ag nitrate is
caustic, thus its concentration should be very low in solution whilst sulfadiazine induces
sensitization and bacterial resistance. Moreover both nitrate and sulfadiazine affect the healing
process [10].
Nanotechnology has provided new chemical forms of silver, such as nanocrystalline silver, for the
employment in biological systems. These formulations can be used to prepare creams, impregnated
and advanced Ag-based medications. The employment of metals such as silver represents the future
in the design of advanced medical systems with antiseptic activity.
[5] Approccio terapeutico alle lesioni cutanee: la gestione della carica batterica - www.riparazionetissutale.it
[6] Angela Peghetti, Matilde Mantovani, Giuliana Canova, Loretta Ferri “Le medicazioni avanzate per il trattamento delle ferite
acute e croniche - Dalle evidenze della letteratura alla pratica quotidiana” – pagg. 9 e seguenti. Servizio Sanitario Regione Emilia
Romagna – Febbraio 2012
[7] Andrea Travan et al “Non-cytotoxic Silver Nanoparticle-Polysaccharide Nanocomposites with Antimicrobial activity”
Biomacromolecules 2009, 10, 1429–1435 1429
[8] Mayer C. Treatment of large human burns with 0.5% silver nitrate solution. Arch Surg 1965:90; 8120-820
[9] Fox C. silver sulfadiazine: a new topical therapy for Pseudomonas in burns. Arch Surg 1968:96; 1840-187
[10] Demling R, DeSanti L. Effect of silver on wound management. Wounds 2001:13
146
This project aims to provide nano-structured biomaterials containing either biologically active ionic
metal or nanostructured forms of silver embedded inside natural polymeric matrices in order to
obtain an absolutely innovative multifunctional system activity.
The aim of this project is to develop novel Ag-based systems suitable for the employment in
advanced dressings for infected non-healing skin wounds. The main objectives for the three years
are listed below:
1. Synthesis of nano-engineered polysaccharides conjugated with biological molecules to
increase their bioactivity and biocompatibility
2. Physical-chemical characterization of conjugated compounds
3. Biological characterization of conjugated compounds
4. Development of polysaccharide-based system synthesized at step 1 containing silver ions and
silver nanoparticles
5. Combinations of aforementioned conjugates with biologically active polysaccharide matrices,
such as hyaluronic acid or other glycosaminoglycans to obtain three-dimensional networks
6. Study of antibacterial and tissue regeneration property of biomaterials developed from step 1
to 4
These novel matrices will constitute a new generation of devices in which they will combine
synergistically the advantages of the individual components, namely:
- Biological activity of molecules, such as lipoic acid and butyrate
- Antiseptic activity of Silver
- Skin regenerative activity of the polysaccharide matrices
• Selection and synthesis of the components of polysaccharide derivatives
• Preliminary biological studies on primary cells using derivates at different degrees of
substitution and concentration
• Set up of experimental procedures for the development of nano-engineered systems containing
silver ions and silver nanoparticles.
Research project
Task1
Synthesis of nano-engineered polysaccharides conjugates
147
Natural polysaccharides such as hyaluronic acid will be nano-engineered by covalent grafting of
bioactive molecules (butyrate and lipoate) with the aim to introduce additional bio-functional
molecules and signals able to increase / modify the bioactivity of the polymer.
Task 2
Physical-chemical characterization of conjugated compounds
Functionalized conjugates will be characterized in order to define degree of substitution, purity,
rheological properties, solubility and miscibility.
Task 3
Biological characterization of conjugated compounds
Primary human cells such as dermal fibroblasts and keratinocytes will be used to study the effects
of the compounds. Cytotoxicity, proliferation, migration, expression of phenotypic markers and of
extracellular matrix components will be considered. Moreover, the influence of the polymers on the
expression and release of pro- and anti-inflammatory cytokines from monocytes and macrophages
will be evaluated in order to clarify their effects on the inflammatory response.
Task 4
Development of polysaccharide-based systems synthesized at step 1 containing silver ions and
The polymers produced in Task 1 will be used as soluble support matrices for silver ions and/or for
silver nanoparticles in order to develop a system endowed with antibacterial activity. Particular
attention will be devoted to characterize dimensions, dispersion and stability of the nanoparticles in
the polymeric colloidal solutions.
Task 5
Combinations of conjugates with biologically active polysaccharide matrices, such as hyaluronic
acid or other glycosaminoglycans to obtain three-dimensional networks
Three dimensional matrices will be obtained combining bioactive/biocompatible polymers with
gelling properties with the silver-containing polymers.
Task 6
Study of antibacterial properties and cytotoxicity of biomaterials
Antibacterial tests will be performed both on silver-containing polymeric solutions and on threedimensional matrices using bacterial strains directly involved in skin infections (Staphylococcus
aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa). The effect will be evaluated
148
both on bacterial planctonic forms and on bacterial biofilms. Particular attention will be provided to
study the possible cytotoxic effects by silver nanoparticles.
1st year
2nd year
3rd year
Synthesis of nano-engineered polysaccharides conjugates
Physical-chemical characterization of conjugated compounds
Biological characterization of conjugated compounds
Development of polysaccharide-based systems containing silver ions and
Combinations of conjugates with biologically active polysaccharide
matrices
Study of antibacterial properties of biomaterials
In the first year my educational activity will regard:
Participation to seminars, conferences
Study of scientific literature
“Biomateriali e Ingegneria Tissutale”, course of the Master Degree in Medical Biotechnology,
Dr. Gianluca Turco (56 hours)
Other courses or school activities
149
FRANCESCA SCOGNAMIGLIO
Title of the thesis: “Nano-engineered adhesive biomaterials for biomedical applications”
Laboratory: 113, building Q
Tutors: Andrea TRAVAN, Ivan DONATI.
Polysaccharide-based adhesive systems
Adhesive systems are largely investigated for several biomedical applications; several innovative
approaches are based on molecular strategies engineered at the nano-scale. An ideal adhesive
system for medical use should allow a proper adhesion between the biomaterial and the biological
tissue avoiding both sliding effects on wet surfaces and adverse reactions. Adhesion can be
achieved through the establish of covalent bonds between the material and a biological surface, or
by exploiting hydrophobic/hydrophilic features of both of them. In general, given the different
composition of the two interacting surfaces, several applications require the of glues and sealants
that allow the proper adhesion. Polysaccharide molecules represent an ideal substrate for the
achievement of biological glues, since they can be easily modified through the addition of chemical
reactive groups. For instance, chitosan, alginate and dextran are prone to be modified at the
nanoscale level for the development of nano-engineered biocompatible adhesive systems.
Biopolymeric membranes for colorectal applicartions
During this research project, biopolymeric membranes will be developed and characterised as a new
biomaterial for anastomotic leakage prevention, in cases of colorectal cancer (CRC) resection.
Colorectal cancer (CRC) is the second most common form of cancer in Europe and its treatment is
mainly based on surgical intervention, with the resection of the affected bowel and the suture of the
extremities to restore the normal bowel transit1. The site at which the bowel continuity is restored is
called anastomosis. The most frequent post-operative complication is the Anastomotic Leakage
(AL) which occurs when no proper and rapid regeneration of the intestinal tissue occurs at the site
of the anastomosis2. Both systemic and surgical factors contribute to the onset of AL leading to
clinical consequences such as generalized peritonitis (requiring abdominal reoperation), increasing
in local recurrence of cancer and decreasing in long term patient survival3.
For these raisons, it has been proposed the use of an external reinforcement that wraps the
anastomosis and trigs the tissue healing4. In this regard, this project aim to develop a material that
wraps the anastomosis and promote the physiological process of tissue regeneration. This material
will be applied by surgeons to the staple line as an external reinforcement. According to
150
laparoscopic techniques, the material should be produce in form of an adhesive patch,
biocompatible, water swellable, easy to handle, and it should not lead to adverse reactions, such as
inflammation, citotoxicity and infection, or causing adhesion between viscera and abdominal walls.
The introduction of nano-fillers and reinforcement will be studied in order to modulate the
mechanical properties of the patches. An additional feature of such material would be its
antibacterial activity.
Polysaccharide-based adhesive for restorative dentistry
Polysaccharide-based adhesive systems are suitable to be employed in dental field. Several
materials based on polysaccharides are investigated for this medical application. Given the high
incidence of oral diseases such dental caries, there is a strong need to restore the dental structure,
thus preventing the onset of secondary ones. At present, materials used for dental restoration are
based on resin composites that are bound to the collagen dentin through an adhesive system. Such
adhesive systems form a hybrid layer on the dental surface that ensures a micromechanical
interlocking. However, the creation of a stable bond between dentin collagen and restorative
material is challenging, given the action of degradation phenomena5 and the lack of chemical bond
between collagen fibrils and resin. On these basis, this research project aims also to develop a new
adhesive system that allows proper interaction between the two surfaces. The adhesion strategy will
be based on the modification at nanoscale level of chitosan, a polysaccharide that has intrinsic
bioadhesive features.
The final goal of this PhD is to develop new nano-engineered adhesive solutions based on natural
polysaccharides for two different applications: one for internal surgery and the second one for
dentistry applications.
The first research line will aim at the development of an engineered bioresorbable biomaterial that
will be used for the prevention of the AL. The biomaterial will be based on natural polysaccharides
such as alginate and Hyaluronic acid (HA), polymers already used in several medical applications.
Moreover, HA conjugated with butyric acid (HABut) will be inserted into the formulation, since
butyric acid has been reported to reduce the AL on rat models7.
The second research line points at the development of an adhesive system able to interact with both
dentin collagen and the restorative material used mainly for dental disease. To achieve this goal,
chitosan will be modified in order to obtain a nano-engineered polymer with a double
functionalisation. In this regard, we aim at modifying chitosan through the introduction of both
methacrylate groups and glutamine residues, able to interact with both the dentin collagen and the
resin-based composites, thus creating an hybrid layer that resist to degradation phenomena.
151
The final goals will be reached through the following intermediate objectives:
¾ Mechanical and physico-chemical characterization of the developed materials;
¾ Definition of adhesive strategies to allow a proper tissue-material adhesion;
¾ In vitro tests for the evaluation of biocompatibility, cell vitality and viability,
proliferation, adhesion and other biological effects;
¾ In vivo studies of the biomaterials on animal models (for the intestinal membrane
research line).
The objectives to be achieved during the first year are the following:
¾ Literature survey on the state of the art about the research topics proposed;
¾ As to the first research line, definition of adhesive strategies to allow a proper tissuematerial bondage. The strategies will be based on the analysis of the adhesive systems
already used in surgical intervention, as well as the development on new ones, based on
the modification at nanometric scale and functionalisation of the polysaccharide matrix,
in order to allow the conjugation with other chemical compounds. Sealants already
available for medical applications will be tested, as well as the use of nano-engineered
polysaccharides;
¾ As to the second research line, a suitable functionalization of the polysaccharide
(chitosan) will be tackled in order to endow the polymer with adhesive properties
towards both restorative resin and collagen fibers;
¾ Phisical, chemical and mechanical characterisation of the biomaterials;
¾ In vitro biological tests in order to investigate the biological activity of the patches.
¾ In vitro biological tests to evaluate the biocompatibility of the functionalized-chitosan
adhesive system.
Research project
Line 1: Development of nano-structured adhesive patches for colorectals applications
The biomaterial that will be developed will be based on a mixture of polysaccharides, such as
alginate and hyaluronic acid, polymers that are already use in several medical applications. In
addition, butyrate will be added in a conjugated form with HA (hyaluronic acid butyric ester), to
confer additional properties to the material. Each polymer exerts a specific biological function and
152
represent an alternative to synthetic polymers or to proteins such as collagen which can be
associated to a severe risk of biological contamination.
The patches will be obtained through the freeze drying procedure and designed to adhere to the
intestinal serosa and to remain in situ for a sufficient period to achieve the cicatrisation and
remodelling of the anastomosis (approximately two weeks). The proper adhesion of the patch to the
intestinal epithelium is required in order to trigger the biological healing processes. The absence of
a proper adhesion may lead to poor biological response. Thus, the development of an efficient
strategy to ensure a proper adhesion is a fundamental aspect for this biomedical applications.
In order to improve the adhesion of the patch to the external intestine walls, several strategies will
be pursued. Furthermore, this material should possess adequate mechanical properties (which can
be tailored upon addition of nanofillers and micro/nano fibres) and should not cause adverse
reactions.
1. Development of adhesion strategies that allow the proper adhesion of the patch to the
intestinal epithelium. In order to improve the adhesion of the patch to the external intestine
walls, several strategies will be pursued.
•
Development of an adhesive system based on molecular strategies that ensure a
proper tissue-material bondage at the nanoscale level. Various approaches will be
pursed, including the possibility to obtain covalent bonding between the
polysaccharide matrix and the tissue. The polysaccharide matrix will be
functionalised to allow the conjugation with adhesive molecules such as dopamine;
•
A combination of already existing adhesion strategies, such as fibrin glue, gelatine
based tissue adhesive, chitosan sealant will be tested;
•
The adhesivness of the different formulations will be tested on intestinal pig
ephitelium.
2. Polysaccharide chemical functionalization. Polymers used to develop the patches will be
chemically modified in order to modulate the adhesive properties of the biomaterial. These
molecules will be functionalised through the introduction of specific pendent groups that
will be chemically bound on polysaccharide backbones, thus creating polymers modified at
nano-scale level. This strategy aims to the formation of side chains that will be able to bind
covalently to tissues.
153
3. Mechanical and physical-chemical characterisation. The patches should posses adequate
mechanical properties in order to withstand handling and positioning stress, to withstand the
peristaltic movements and should not cause stenosis at the anastomosis site upon material
stiffening. These aspects will be characterised through mechanical tests considering ultimate
strength, Young’s Modulus, deformation at break of patches in both wet and dried
conditions.
4. Introduction of a structural reinforcement in the polysaccharide matrix.
• Various options for the reinforcement of the patches will be investigated, in order to
improve its mechanical performances in physiological conditions. The introduction of
nanofiller and nanofibers capable of increasing mechanical properties will be studied.
5. In vitro biological tests will assay:
• Cytocompatibility of the components by LDH and MTT assays.
• Cell viability and activity after incubation of cells with the patch (AlamarBlue, MTT
assays);
• Biocompatibility of the patches by exposing human monocytes to the sterile patch for a
predeterminated length of time and evaluation of inflammation response through the
detection of cytokines into the medium by MultiPlex beads;
• Cell growth, apoptosis, differentiation, synthesis of extracellular matrix quantified through RT-
PCR and western blot analysis.
6. In vivo studies of patches on animal models. These tests will be performed by a research partner of
the EU project AnastomoSEAL (University of Maastricht); results will be shared and anlyzed by the
whole project consortium, allowing to gather information about the behaviour of the developed
membranes in an animal model (pig).
The Gantt chart of this research line is sketched in the following table.
1st year
2nd year
3rd year
Survey literature on adhesive systems
Material selection
Material development (chemical, mechanical and structural
characterisation)
In vitro biological tests
In vivo tests
154
Line 2: Development of nano-structured adhesive patches for restorative dentistry
In order to improve the adhesion between the dental surface and the restorative material we aim to
develop a nano-engineered dental adhesive system capable to form covalent chemical bonds with
both the dentin collagen and the resin. This system will be based on a natural polysaccharide
(chitosan) that will be modified in order to bear both methacrylate groups and glutamine residues.
Methacrylate groups guarantee the bound between the adhesive and the restorative material by a
photopolymerisation process, while glutamine residues are able to bind the exposed dentin collagen
through crossilinking reaction catalysed by transglutaminase enzymes.
1.
Synthesis of the adhesive system and mechanical, physical and chemical characterisation.
•
Introduction of flanking methacrylate and glutamine residues on the polysaccharide
chain;
•
Morphological characterisation through the use of microscopy techniques (SEM),
and infrared spectroscopy (ATR-FTIR);
•
2.
Viscosity and solubility chracterization of the adhesive system;
•
Biocompatibility (LDH, MTT) using fibroblasts cell lines as well as primary cultures
of gingival and pulp fibroblast and odontoblasts;
•
Analysis of the effect on metalloproteinase activity.
The Gantt chart of this research line is sketched in the following table.
1st year
2nd year
3rd year
Synthesis of the adhesive and physical-chemical
characterization
Material development
(mechanical and structural
characterisation of the adhesive interface)
Nanobiotechnology ( Modulus B course of the degree in Medical Biotechnology)
Interdisciplinary PhD Spring School on nanotechnology
155
Other schools and courses
Laboratory activities aimed to improve the knownledge in the field of chemistry,
engineereing and biology field.
Reference list
1
Rajput Ashwani and Bullard Dunn Kelli, "Surgical Management of Rectal Cancer,"in
34
ed.2007), pp.241-249.
2
Y. H. Ho and M. A. Ashour, "Techniques for colorectal anastomosis," World J Gastroenterol.
16(13), 1610 (2010). Ref Type: Journal
3
C. S. McArdle, D. C. McMillan, and D. J. Hole, "Impact of anastomotic leakage on long-term
survival of patients undergoing curative resection for colorectal cancer," Br. J Surg 92(9), 1150
(2005). Ref Type: Journal
4
J. Hoeppner, et al., "Small intestinal submucosa for reinforcement of colonic anastomosis," Int J
Colorectal Dis 24(5), 543 (2009). Ref Type: Journal
5
L. Breschi et all. “Dental adhesion review: aging and stability of the bonded interface”. Dent
Mater 2008 24 (1), 90-101
156
Candidate CRISTIAN SVETINA
Title of the thesis: Photon beam studies and characterization for EUV/Soft
X-ray coherent imaging of nano-structures using coherent FEL radiation
Laboratory: ELETTRA-Sincrotrone Trieste
Supervisor: Dr. Marco Zangrando
Tutors (if any):
Fermi@Elettra is the first seeded Free Electron Laser in the EUV/Soft Xray energy range. Based on a High Gain Harmonic Generation (HGHG)
scheme, this FEL generates Fourier transform limited sub-picosecond
pulses, with a high photon flux (up to 1014 photon/pulse), and variable
polarization [1].
This unique radiation source will be used for time-domain spectroscopy
and coherent imaging experiments, therefore paving the way for studying
the dynamics of systems out of equilibrium or for the microscopy of nanoobjects by coherent imaging techniques using variable polarization fully
coherent soft X-ray ultrashort pulses. The Diffraction and Projection
Imaging (DiProI) beamline is one of the four end-stations operating with
the FERMI@Elettra. In particular, DiProI is devoted to the imaging of
both fixed and free-standing nano-objects of various nature [2]. In the first
operating stage of FERMI, DiProI will perform Coherent Diffraction
Imaging (CDI), in-line and Fourier transform holography of nanostructures, while projection imaging and stereo imaging are planned to be
implemented in the next future. One of the DiProI main goals is to perform
resonant CDI, with dichroic contrast obtained by varying the FEL
wavelength and polarization, suitable for magnetism studies. In CDI the
real- space information of the specimen is extracted from the far field
diffraction pattern by an iterative phase retrieval algorithm.
The main advantage of using FEL pulses, instead of a synchrotron source,
arises from the possibility of acquiring instantaneously the information of
the local electron density in the sample or its magnetic images (using
variable polarization) with a very high spatial resolution (~1μm) during the
time duration of the FEL pulses (~100 fs). The detection of the pulses will
be provide by a technique called Command Detector Interface that has
been validated by A. Barty and coworkers [3] in an experiment at the
157
FLASH FEL of DESY. Furthermore, in a recent work C. Gutt and
coworkers have performed
x-ray magnetic scattering on a Co/Pt multilayer film by tuning the FEL
wavelength radiation across the Co absorption edge [4]. In order to be able
to achieve these goals, DiProI needs to focus the FEL Gaussian photon
beam below 10 μm (FWHM), with high spatial coherence and fluence. For
reaching such a behavior two solutions could be adopted: implement a
focusing lens (i.e., a Fresnel zone plate) or use focusing mirrors with
suitable profiles. In the first case the resolution is mainly imposed by the
limited quality of the used optical elements in full field and scanning X-ray
microscopy. Moreover, the lenses can be easily damaged by the high
fluence emitted by the FEL. The lensless CDI by means of focusing
mirrors, on the other hand, has been demonstrated to be a powerful
microscopy method [5] and, nowadays, has become the commonly used
solution for FEL sources, allowing to resolve nano-structures with high
resolution [6]. For these reasons the DiProI focusing system consists of a
set of Kirkpatrick- Baez (KB) active mirrors [7] capable of modifying the
focused beam dimensions, the focal length and, in principle, the wavefront
quality, providing high resolution CDI. The diagnostics of focused photon
beams is of great interest in the FEL community since they are essential
for reaching the needed spot qualities and it is the core of the proposed
project. Moreover, the optimization of the spot properties can be done by
exactly knowing the effects of the focusing mirrors on the radiation. For
this reason an accurate metrology, ex-situ and in-situ, is very important in
general, and for DiProI in particular, for optimizing the light spot on the
sample.
References
[1] E. Allaria et al., “Highly coherent and stable pulses from the FERMI
seeded free-electron laser in the extreme ultraviolet”, Nature Photonics 6,
699–704 (2012)
[2] E. Pedersoli et al., “Multipurpose modular experimental station for the
DiProI beamline ofFermi@Elettra free electron laser”, Rev. Sci. Instrum.
82, 043711 (2011)
[3] A. Barty et al., “Ultrafast single-shot diffraction imaging of nanoscale
dynamics”, Nature Photonics 2 415–19 (2008)
[4] C. Gutt, et al., “Single-pulse resonant magnetic scattering using a soft
x-ray free-electron laser”, Physical Review B 81 100401 (2010)
[5] J. Miao et al., ”Extending the methodology, of X-ray crystallography to
158
allow imaging of micrometre-sized non-crystalline specimens,”, Nature
400, 342-344 (1999)
[6] H. N. Chapman et al., “Femtosecond Diffractive Imaging with a SoftX-ray Free-Electron Laser”, Nature Physics 2, 839-843 (2006)
[7] L. Raimondi et al., “Microfocusing of the FERMI@Elettra FEL beam
with a K-B active optics system: spot size predictions by application of the
WISE code”, NIM-A special issue IWXM, Article in Press (2012)
We plan to investigate the active KB system performances in terms of
metrology measurements, focusing simulations, and actual measurements
using the FERMI FEL radiation. The metrology part will be divided into
ex-situ and in-situ metrology. The former will be carried out at the Elettra
metrology laboratory by means of a Long Trace Profiler (LTP) and an
interferometer, while the latter will be pursued by applying the pencil
beam and the lateral shearing imaging techniques, using the FEL radiation.
The results obtained during the metrological investigations will be used for
predicting the KB performances at the DiProI beamline. These simulations
will be done using ray tracing and wavefront propagation codes.
During the measurements with the FEL radiation we plan to apply
different techniques that allow the determination of the focused spot
dimensions and the wavefront, like the Hartmann sensor, some phase
retrieval methods, the ablation of some suitable samples (like PMMA and
Silicon), and the direct imaging by means of YAG crystals and
phosphorous screens.
Finally, a significant part of the project work will be dedicated to the first
experiments performed by the advanced optical devices described above,
characterized and optimized by the proponent of this thesis.
We plan to perform the first ex-situ metrological investigation of the active
KB mirrors in order to measure the best reachable longitudinal profiles.
We plan to simulate the optical performances of the active KB system
using ray tracing and wavefront propagation codes.
We will begin to characterize the FEL radiation focused with the active KB
by means of a wavefront sensor and PMMA/Si ablation.
Research project
The research activities will be divided into two different categories:
159
active KB mirrors figure optimization
measurement of the focused radiation in terms of spot size and
shape
The figure optimization will be performed by means of metrology
measurement techniques, both involving ex-situ and in-situ metrology:
ex-situ metrology will be carried out in the Elettra metrology
laboratory
in-situ metrology will performed using the FERMI FEL radiation
The FEL focused spot will be characterized in the DiProI end-station by
means of the following techniques:
Wavefront Hartmann sensor detector
direct imaging of YAG and Phosphorous screens
PMMA and Silicon ablation
Phase retrieval methods
Attend the annual meetings and seminars organized by the School
of nanotechnology
Attend some seminars related to the FEL optics and diagnostics
Support at the ELETTRA metrology laboratory
Study of several FEL optics books and review articles
Attend some International conferences about FEL optics and
diagnostics
160
SIMONE VELARI
Title of the thesis: “Modeling the atomic scale properties of simple biomolecules at surfaces: from
the role of solvent to small structured peptides”
Laboratory: /
Supervisor: Alessandro De Vita
Tutors (if any): /
The control of a large number of processes in living organisms is based on the recognition of small
organic molecules by large molecules (such as proteins, enzymes or antibodies) through a specific
interaction site. For this reason, the design of short, peptide-based artificial receptors capable of
highly specific recognition is one of the goals of biotechnology. For instance, small synthetic
peptides have been recently designed as biosensor transducers, and their functionality is intimately
related to their conformation, in particular their secondary structure (such as α-helix or β-sheet),
since it is known to allow for intermolecular self-organization of the sensing peptides over the
sensor surface.
In the perspective of an atomic scale investigation of biomolecules by SPM, it is crucial, although
not trivial, to be able to deposit the molecules on a surface while preserving their secondary
structure, as evident from the above discussion. A simple deposition method, albeit poorly tested up
to now with respect to the preservation of the secondary structure, could be the deposition from
solvent drop in ambient conditions. However, regardless of the chosen method, the role of the
solvent upon interaction with the surface cannot be neglected, and must be thoroughly investigated.
Preliminary STM experiments at liquid helium temperature, carried out within the group of Prof.
Giovanni Comelli by Dr. Carlo Dri at the IOM-CNR TASC Laboratory in Basovizza, show
evidence that solvent molecules create complex self-assembly structures upon adsorption, that in
some cases even mimic the structural properties of small peptides.
Theoretical modeling using the ab-initio computational approach is crucial in order to successfully
address these questions, to provide insight not experimentally available due to the fundamental
limitation of STM, i.e. the lack of chemical resolution. The aim of this project is to provide a
theoretical model that could help rationalise the experimental data.
To formulate a well-defined adsorption model for different solvent molecules (e.g. DMSO, TFE,
acetonitrile, phosphate buffer, etc.), all the interactions between surface and solvent, and the
possible inter-molecular bonds have to be explored. In particular, the relative importance of
hydrogen bonding and dispersive interactions for the self-assembly have to be assessed. Moreover,
the role and the presence of water molecules must be evaluated, that could indeed be stabilized by
161
coadsorpion within the observed structures. Such a complex pattern of chemical and physical
interactions can be accounted for only by means of quantum mechanical computational techniques,
which are able to correctly model the characteristics of inter-atomic bonds and electrostatic
interactions. Density-functional theory (DFT) therefore represents the ideal choice when dealing
with systems of such size and complexity. The Quantum Espresso code based on DFT, plane waves
basis sets and pseudopotentials will be used for these ab-initio calculations.
As a second step, the adsorption of simple, small synthetic peptides at surfaces with well-defined
secondary structure (e.g. α-helices or β-sheets) will also be modeled with different and larger scale
computational techniques, such as classical Molecular Dynamics or Kinetic Monte Carlo Methods.
The adsorption of different aminoacid sequences will be explored, aiming at highlighting the effects
of peptide size, charge distribution within the peptide structure and chemical affinity of specific
residues with the substrate.
For each step of the project, theoretical simulation of the STM images will be carried out, within
suitable frameworks and approximations, on tAhe basis of the obtained computational results, in
order to directly compare the theoretical models with the experimental data.
The long-term goal of this project will be the study of the interactions between our prototypical
biomolecules (starting from solvents, amino acids up to small peptides) and a variety of other
surfaces with different physical and chemical properties, e.g., insulating thin films grown on metal
substrates such as NaCl @Cu(111), to explore the range of functionalities achievable by our target
peptide-based nanostructures in view of their possible use in bio-nanotechnology.
•
Define the problem, study the literature and understand the theoretical background of the
STM experiment.
•
Choose the more useful computational techniques to understand the experimental data.
•
Model DMSO molecule, find through some tests the more suitable parameters and
pseudopotentials. Try to model the DMSO - Water interaction.
•
Optimize the lattice constant of Gold, comparing the results of different pseudopotentials;
create an Au (111) surface, optimize all the simulation parameters.
•
Create and study a DMSO - Au(111) adsorption model exploring different configurations
and simulation parameters.
•
Try to replicate the self - assembled structures observed in STM images.
•
Evaluate the possible presence of water at the interface through classical and quantum
methods.
•
Simulate STM images to compare the theoretical model to the experimental data.
Research project
1) Interact with the experimental group
2) Optimize the metal surface
162
•Optimize the lattice constant
•Create a slab and optimize the simulation parameters
3) Model the DMSO adsorption on metal surface
•Compare different pseudopotenzials and optimize the simulation parameters
•Model DMSO
•Create an ab-initio adsorption model for a DMSO molecule
•Create an ab-initio model for DMSO self-assembly
•Simulate STM images of the system
•Perform a QM/MM Dynamics on the Gold-DMSO system
4) Model the TFE adsorption on metal surface
•Compare different pseudopotenzials and optimize the simulation parameters
•Model TFE
•Create an ab-initio adsorption model for a TFE molecule
•Create an ab-initio model for TFE self-assembly
•Simulate STM images of the system
•Perform a QM/MM Dynamics on the Gold-TFE system
5) Model the adsorption of amino-acids on metal surface
6) Model small peptides adsorption on metal surface
7) Model other surfaces with different chemical properties
• Participate in a Winter School of QM/MM Molecular Dynamics held at ICTP in Grignano.
• Learn QM/MM codes like AMBER and LAMMPS.
• Attend a course on Intermolecular forces and potentials, organized by Prof. Scoles.
• Attend a course on electron microscopy techniques.
163
5.Elenco dei componenti del collegio docenti
Componente
Ente
AFRICH Cristina IOM‐CNR
Dipartimento
Not assigned
BARALDI University Trieste Department of Physics ‐ DF
Alessandro
BERTI Federico University Trieste Department of Chemical and Pharmaceutical Science ‐ DSCF
BIASIOL Giorgio IOM‐CNR
Advanced Technology and Nanoscience ‐ TASC INFM
BONIN Serena University Trieste Clinical Department of Medical Science, Surgery and Health ‐ DCSMCS
BRESCHI University Trieste Clinical Department of Medical Lorenzo
Science, Surgery and Health ‐ DCSMCS
CADENARO University Trieste Clinical Department of Medical Milena
CASALIS Elettra Not assigned
Loredana
Synchrotron Spa
CESARO Attilio University Trieste Department of Life Science ‐ DLS
COJOC Dan
University Trieste Department of Physics ‐ DF
SSD
FIS/03
ASS
FIS/03
RU
CHIM/06 RU
RIC
MED/34 RU
Ruolo Collegio
External expert
Exclusive Member
Member
Member non academic
Exclusive Member
MED/28 PA
Exclusive Member
MED/28 PA
Exclusive Member
RIC
CHIM/04 PO
IOM‐CNR
COMELLI Giovanni
DA ROS Tatiana University Trieste Department of Chemical and Pharmaceutical Science ‐ DSCF
DE VITA University Trieste Department of Engineering and Alessandro
Architecture ‐ DIA
DI LENARDA University Trieste Clinical Department of Medical Roberto
FASSINA University Padova Not assigned
Ambrogio
FERMEGLIA University Trieste Department of Engineering and Maurizio
Architecture ‐ DIA
FIRRAO University Udine Department of Biology and Plant Giuseppe
Protection ‐ DBPP (UD)
FORNASIERO University Trieste Department of Chemical and Paolo
Pharmaceutical Science ‐ DSCF
FRALEONI Elettra Not assigned
Alessandro
Synchrotron Spa
FRONZONI University Trieste Department of Chemical and Giovanna
Qualifica
RIC
FIS/03
PO
CHIM/08 RU
ING‐
PA
IND/22
MED/28 PO
Member non academic
Exclusive Member
Member non academic
Exclusive Member
Exclusive Member
Exclusive Member
Exclusive Member
MED/08 PO
Member
ING‐
IND/24
AGR/12
PO
Director
PA
Member
CHIM/03 PA
RIC
CHIM/02 PA
Exclusive Member
Member non academic
Exclusive Member
164
Componente
Ente
Dipartimento
SSD
Qualifica
GAMINI Amelia University Trieste Department of Life Science ‐ DLS
CHIM/04 RU
GIRALDI Tullio
University Trieste Department of Life Science ‐ DLS
BIO/14
KISKINOVA Maya
LAZZARINO Marco
LUGHI Vanni
Elettra Synchrotron Spa
IOM‐CNR
MACOR Paolo
PO
Not assigned
RIC
University Trieste Department of Engineering and Architecture ‐ DIA
University Trieste Department of Life Science ‐ DLS
RIC
MARSI Stefano University Trieste Department of Engineering and Architecture ‐ DIA
MARZARI University Trieste Department of Life Science ‐ DLS
Roberto
MORGANTE University Trieste Department of Physics ‐ DF
Alberto
ONESTI Silvia
Elettra Not assigned
Synchrotron Spa
PASQUATO University Trieste Department of Chemical and Lucia
PASSAMONTI University Trieste Department of Life Science ‐ DLS
Sabina
PRATI Ubaldo Fund. Tommaso Not assigned
Campanella
PRICL Sabrina University Trieste Department of Engineering and Architecture ‐ DIA
RUBINI Silvia
IOM‐CNR
SBAIZERO Orfeo University Trieste Department of Engineering and Architecture ‐ DIA
SCAINI Denis
Elettra Not assigned
Synchrotron Spa
SCHMID Chiara University Trieste Department of Engineering and Architecture ‐ DIA
SERGO Valter
University Trieste Department of Engineering and Architecture ‐ DIA
STANTA Giorgio University Trieste Clinical Department of Medical Science, Surgery and Health ‐ DCSMCS
TOFFOLI CRO Aviano
Not assigned
Giuseppe
TORMEN IOM‐CNR
Advanced Technology and Massimo
Nanoscience ‐ TASC INFM
ING‐
RIC
IND/22
MED/04 RIC
ING‐
INF/01
BIO/06
RU
FIS/01
PA
PO
RIC
CHIM/06 PA
BIO/10
RIC
P.Osp
ING‐
IND/24
PA
RIC
ING‐
IND/22
PO
RIC
ING‐
PA
IND/22
ING‐
PO
IND/22
MED/08 PA
P.Osp
RIC
Ruolo Collegio
Exclusive Member
Member non academic
Member non academic
Member non academic
Exclusive Member
Exclusive Member
Exclusive Member
Exclusive Member
Vice Director
Member non academic
Exclusive Member
Exclusive Member
Member non academic
Exclusive Member
Member non academic
Exclusive Member
Member non academic
Exclusive Member
Exclusive Member
Member
Member non academic
Member non academic
165
Componente
UGO Paolo
VACCARI Lisa
Ente
University Venezia
Elettra Synchrotron Spa
Dipartimento
SSD
Qualifica
Not assigned
CHIM/01 PA
Not assigned
RIC
Ruolo Collegio
Exclusive Member
Member non academic
166
6. Produzione scientifica del collegio docenti 2007-2012
Personale di ruolo nelle università italiane e INAF
1. BARALDI Alessandro
• SAVIO L, GERBI A, VATTUONE L, BARALDI A., COMELLI G, ROCCA M (2006). Monitoring super- and subsurface
oxygen on Ag(210) by high energy resolution X-ray photoelectron spectroscopy: Subsurface diffusion and
segregation. JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES
& BIOPHYSICAL, vol. 110; p. 942-, ISSN: 1520-6106
• WESTSTRATE C.J, BAKKER J.W, RIENKS E.D.L, VINOD C.P, LIZZIT S, PETACCIA L, BARALDI A., NIEUWENHUYS
B.E (2006). Synchrotron XPS and desorption study of the NO chemistry on a stepped Pt surface. SURFACE
SCIENCE, vol. 600; p. 1991, ISSN: 0039-6028
• BIANCHETTIN L, BARALDI A., DE GIRONCOLI S, LIZZIT S, PETACCIA L, VESSELLI E., COMELLI G, ROSEI R
(2006). Geometric structure of the N/Rh(100) system by core-level photoelctron spectroscopy: Experiment and
theory. PHYSICAL REVIEW. B, CONDENSED MATTER AND MATERIALS PHYSICS, vol. 74; p. 045430-, ISSN: 10980121, doi: 10.1103/PhysRevB.74.045430
• F. BUATIER DE MONGEOT, A. TOMA, A. MOLLE, S. LIZZIT, L. PETACCIA, BARALDI A. (2006). Carbon monoxide
dissociation on Rh nanopyramids. PHYSICAL REVIEW LETTERS, vol. 97; p. 56103, ISSN: 0031-9007
• BIANCHETTIN L, BARALDI A., VESSELLI E, DE GIRONCOLI S, LIZZIT S, PETACCIA L, COMELLI G., ROSEI R
(2007). Experimental and Theoretical Surface Core Level Shift Study of the S-Rh(100) Local Environment.
JOURNAL OF PHYSICAL CHEMISTRY. C, NANOMATERIALS AND INTERFACES, vol. 111; p. 4003-, ISSN: 1932-7447
• BARALDI A., BIANCHETTIN L, VESSELLI E, DE GIRONCOLI S, LIZZIT S, PETACCIA L, ZAMPIERI G, COMELLI G.,
ROSEI R (2007). Highly under-Coordinated Atoms at Rh Surfaces: Interplay of Strain and Coordination Effects on
Core Level Shift. NEW JOURNAL OF PHYSICS, vol. 9; p. 143-, ISSN: 1367-2630
• BUATIER DE MONGEOT F, TOMA A, MOLLE A, LIZZIT S, PETACCIA L, BARALDI A. (2007). Self-organised
synthesis of Rh nanostructures with tunable chemical reactivity. NANOSCALE RESEARCH LETTERS, vol. 2; p. 251,
ISSN: 1931-7573
• BARALDI A., VESSELLI E., BIANCHETTIN L, COMELLI G, LIZZIT S, PETACCIA L, DE GIRCONCOLI S, LOCATELLI
A, MENTES O.T, ABALLE L, WEISSENRIEDER J, ANDERSEN J.N (2007). The (1x1)-> hexagonal phase transition on
Pt(100) studied by high resolution core level photoemission. THE JOURNAL OF CHEMICAL PHYSICS, vol. 127; p.
164702-, ISSN: 0021-9606, doi: 10.1063/1.2794344
• DING X, DE ROGATIS L, VESSELLI E, BARALDI A., COMELLI G, ROSEI R, SAVIO L, VATTUONE L, ROCCA M,
FORNASIERO P, ANCILOTTO F, BALDERESCHI A, PERESSI M. (2007). Interaction of carbon dioxide with Ni(110): a
combined experimental and theoretical study. PHYSICAL REVIEW. B, CONDENSED MATTER AND MATERIALS
PHYSICS, vol. 76; p. 195425-(1-12), ISSN: 1098-0121, doi: 10.1103/PhysRevB.76.195425
• TAIT S.L, WANG Y, LIN N, COSTANTINI G, BARALDI A., ESCH F, LIZZIT S, PETACCIA L, KERN K (2008). MetalOrganic coordination interactions in Fe-Teraphthalic acid networks on Cu(100). JOURNAL OF THE AMERICAN
CHEMICAL SOCIETY, vol. 130; p. 2108-2113, ISSN: 0002-7863
• BARALDI A. (2008). Structure and chemical reactivity of transition metal surfaces as probed by synchrotron
radiation core-level photoelectron spectroscopy. JOURNAL OF PHYSICS. CONDENSED MATTER, vol. 20; p. 93001,
ISSN: 0953-8984
• L. BIANCHETTIN, BARALDI A., S. DE GIRONCOLI, E. VESSELLI, S. LIZZIT, L. PETACCIA, G. COMELLI, R. ROSEI
(2008). Core level shifts of under-coordinated Pt atoms. THE JOURNAL OF CHEMICAL PHYSICS, vol. 128; p.
114706-, ISSN: 0021-9606
• E. VESSELLI, L. BIANCHETTIN, BARALDI A., A. SALA, G. COMELLI, L. PETACCIA, S. LIZZIT, S. DE GIRONCOLI
(2008). The Ni3Al(111) surface structure: experiment and theory. JOURNAL OF PHYSICS. CONDENSED MATTER,
vol. 20; p. 195223-, ISSN: 0953-8984
• T. STAUDT, A. DESIKUSUMASTUTI, M. HAPPEL, E. VESSELLI, BARALDI A., S. GARDONIO, S. LIZZIT, F. ROHR
AND J. LIBUDA (2008). Modelling NOx storage meterials: a high-resolution photoelectron spectroscopy study on
the interaction of NO2 with Al2O3/NiAl(110) and BaO/Al2O3/NiAl(110). JOURNAL OF PHYSICAL CHEMISTRY. C,
NANOMATERIALS AND INTERFACES, vol. 112; p. 9835-, ISSN: 1932-7447
• VESSELLI E, CAMPANIELLO M, BARALDI A., BIANCHETTIN L, AFRICH C, ESCH F, LIZZIT S, COMELLI G. (2008). A
Surface Core Level Shift Study of Hydrogen-Induced Ordered Structures on Rh(110). JOURNAL OF PHYSICAL
CHEMISTRY. C, NANOMATERIALS AND INTERFACES, vol. 112; p. 14475-, ISSN: 1932-7447
• VESSELLI E, DE ROGATIS L, DING X, BARALDI A., SAVIO L, VATTUONE L, ROCCA M, FORNASIERO P, PERESSI
M., BALDERESCHI A, ROSEI R, COMELLI G (2008). Carbon Dioxide Hydrogenation on Ni(110). JOURNAL OF THE
AMERICAN CHEMICAL SOCIETY, vol. 130(34); p. 11417-11422, ISSN: 0002-7863, doi: 10.1021/JA802554G
• S. LIZZIT, Y. ZHANG, K.L. KOSTOV, L. PETACCIA, BARALDI A., R. LARCIPRETE, D. MENZEL, K. REUTER (2009).
O- and H- induced surface core level shifts on Ru(0001): prevalence of the additivity rule. JOURNAL OF PHYSICS.
CONDENSED MATTER, vol. 21; p. 134009, ISSN: 0953-8984
• M. BIANCHI, D. CASSESE, A. CAVALLIN, R. COMIN, F. ORLANDO, L. POSTREGNA, E. GOLFETTO, S. LIZZIT,
BARALDI A. (2009). Clean and oxygen induced surface core level shift on Ir(111). NEW JOURNAL OF PHYSICS, vol.
11; p. 063002, ISSN: 1367-2630
• BIANCHETTIN L, BARALDI A., DE GIRONCOLI S, VESSELLI E, LIZZIT S, COMELLI G., ROSEI R (2009). Surface
Core Level Shift: High Sensitive Probe to Oxygen-Induced Reconstruction of Rh(100). JOURNAL OF PHYSICAL
• S. LIZZIT, BARALDI A., CH. GRÜTTER, J.H. BILGRAM, PH. HOFMANN (2009). The surface phase transition and
low temperature phase of α-Ga(010) studied by SPA-LEED. SURFACE SCIENCE, vol. 603; p. 3222, ISSN: 00396028
167
• P. LACOVIG, M. POZZO, D. ALFÈ, P. VILMERCATI, BARALDI A., S. LIZZIT (2009). Growth of dome-shaped carbon
nanoislands on Ir(111): the intermediate between carbidic clusters and quasi free-standing graphene. PHYSICAL
REVIEW LETTERS, vol. 103; p. 166101, ISSN: 0031-9007
• DE ROGATIS L, VESSELLI E, BARALDI A., CASULA M.F, MONTINI T, COMELLI G, GRAZIANI M, FORNASIERO P.
(2009). Charge Redistribution at the Embedded Rh-Alumina Interface. JOURNAL OF PHYSICAL CHEMISTRY. C,
NANOMATERIALS AND INTERFACES, vol. 113(42); p. 18069-18074, ISSN: 1932-7447, doi: 10.1021/JP905736Q
• GOLFETTO E., BARALDI A., POZZO M., ALFÈ D., SALA A., LACOVIG P., VESSELLI E., LIZZIT S., COMELLI G.,
ROSEI R. (2010). Determining the chemical reactivity trends of Pd/Ru(0001) pseudomorphic overlayers: core level
shift measurements and DFT calculations. JOURNAL OF PHYSICAL CHEMISTRY. C, NANOMATERIALS AND
INTERFACES, vol. 114 (2010); p. 436--, ISSN: 1932-7447
• BALOG R., JORGENSEN B., NILSSON L., ANDERSEN M., RIENKS E., BIANCHI M., FANETTI M., LAESGAARD E.,
BARALDI A., LIZZIT S., SLJIVANCANIN Z., BESENBACHER F., HAMMER B., PEDERSEN T.G. HOFMANN PH.,
HORNEKAER L. (2010). Band Gap Opening in Graphene Induced by Patterned Hydrogen Adsorption. NATURE
MATERIALS, vol. 9 (2010); p. 315-319, ISSN: 1476-1122
• VESSELLI E, RIZZI M, DE ROGATIS L, DING X, BARALDI A., COMELLI G, SAVIO L, VATTUONE L, ROCCA M, P.
FORNASIERO, BALDERESCHI A, PERESSI M (2010). Hydrogen-assisted transformation of CO2 on nickel: the role of
formate and carbon monoxide. THE JOURNAL OF PHYSICAL CHEMISTRY LETTERS, vol. 1; p. 402-406, ISSN: 19487185, doi: 10.1021/jz900221c.
• LIZZIT S., ZAMPIERI G., PETACCIA L., LARCIPRETE R., LACOVIG P., RIENKS E.D.L., BIHLMAYER G., BARALDI A.,
HOFMANN PH. (2010). Band dispersion in the deep 1s core levels of graphene. NATURE PHYSICS, vol. 6; p. 345349, ISSN: 1745-2473, doi: 10.1038/nphys1615
• LIZZIT S., BARALDI A. (2010). High resolution fast x-ray photoelectron spectroscopy study of ethylene
interaction with Ir(111): from chemisorption to dissociation and graphene formation. CATALYSIS TODAY, vol. 154;
p. 68-74, ISSN: 0920-5861, doi: 10.1016/j.cattod.2010.05.028
• VESSELLI E., BARALDI A., LIZZIT S., COMELLI G. (2010). Large Interlayer Relaxation at a Metal-Oxide
Interface:
The Case of a Supported Ultrathin Alumina Film. PHYSICAL REVIEW LETTERS, vol. 105; p. 046102-046105, ISSN:
0031-9007, doi: 10.1103/PhysRevLett.105.046102
• POZZO M., ALFE`D., LACOVIG P., HOFMANN PH., LIZZIT S., BARALDI A. (2011). Thermal Expansion of
Supported and Freestanding Graphene:
Lattice Constant versus Interatomic Distance. PHYSICAL REVIEW LETTERS, vol. 106; p. 135501-135504, ISSN:
0031-9007, doi: 10.1103/PhysRevLett.106.135501
• MINIUSSI E., POZZO M., BARALDI A., VESSELLI E., ZHAN R.R., COMELLI G., MENTES T.O., NINO M.A.,
LOCATELLI A., LIZZIT S., ALFE' D. (2011). Thermal Stability of Corrugated Epitaxial Graphene Grown on Re(0001).
PHYSICAL REVIEW LETTERS, vol. 106/2011; p. 216101-216104, ISSN: 0031-9007, doi:
10.1103/PhysRevLett.106.216101
2. BONIN Serena
• TOTHOVA SM, BONIN S., TREVISAN G., STANTA G. (2006). Mycosis fungoides: is it a Borrelia burgdorferiassociated disease?. BRITISH JOURNAL OF CANCER, vol. 94; p. 879-883, ISSN: 0007-0920
• BONIN S., BRUNETTI D, BENEDETTI E, GORJI N, STANTA G. (2006). Expression of cyclin-dependent kinases and
CDC25a phosphatase is related with recurrences and survival in women with peri- and post-menopausal breast
cancer. VIRCHOWS ARCHIV, vol. 448; p. 539-544, ISSN: 0945-6317
• STANTA G., POZZI MUCELLI S, PETRERA F, BONIN S., BUSSOLATI G (2006). A Novel Fixative Improves
Opportunities of Nucleic Acids and Proteomic Analysis in Human Archive's Tissues. DIAGNOSTIC MOLECULAR
PATHOLOGY, vol. 15(2); p. 115-123, ISSN: 1052-9551
• SCAGGIANTE B., BONIN S., CRISTIANO L, SIRACUSANO S, STANTA G, DAPAS B, GIANSANTE C, FIOTTI N,
GRASSI G (2008). Prostate Tumour-Inducing gene-1 analysis in human prostate cancer cells and tissue in relation
to Mycoplasma infection. CANCER INVESTIGATION, vol. 26(8); p. 800-808, ISSN: 0735-7907
• BONIN S., BRUNETTI D, BENEDETTI E, DOTTI I, GORJI N, STANTA G (2008). Molecular characterisation of breast
cancer patients at high and low recurrence risk. VIRCHOWS ARCHIV, vol. 452; p. 241-250, ISSN: 0945-6317, doi:
10.1007/s00428-007-0570-9
• STANTA G, CESCATO A, BONIN S., BARBAZZA R (2008). BIOETHICS CONSIDERATIONS FOR MEDICAL
RESEARCH IN HUMAN ARCHIVE TISSUES: THE POINT OF VIEW OF THE RESEARCHER. VIRCHOWS ARCHIV, vol.
453; p. 117-119, ISSN: 0945-6317
• TREVISAN G, PADOVAN C, SCAINI MT, CINCO M, FLORIS R, BONIN S. (2008). Anetoderma Associated with
Lyme Disease: A Case Report. ACTA DERMATO-VENEREOLOGICA, vol. 88(5); p. 536-538, ISSN: 0001-5555, doi:
10.2340/00015555-0513
• NARDON E, DONADA M, BONIN S., DOTTI I, STANTA G (2009). Higher random oligo concentration improves
reverse transcription yield of cDNA from bioptic tissues and quantitative RT-PCR reliability. EXPERIMENTAL AND
MOLECULAR PATHOLOGY, vol. 87; p. 146-151, ISSN: 0014-4800, doi: 10.1016/j.yexmp.2009.07.005
• DOTTI I., BONIN S., BASILI G., NARDON E., BALANI A., SIRACUSANO S., ZANCONATI F., PALMISANO S., DE
MANZINI N., STANTA G. (2010). Effects of formalin, methacarn, and fineFIX fixatives on RNA preservation.
DIAGNOSTIC MOLECULAR PATHOLOGY, vol. 19; p. 112-122, ISSN: 1052-9551
• BONIN S., MIERTUSOVA TOTHOTOVA S., BARBAZZA R., BRUNETTI D., STANTA G., TREVISAN G. (2010).
Evidence of Multiple Infectious Agents in Mycosis Fungoides Lesions. EXPERIMENTAL AND MOLECULAR
PATHOLOGY, vol. 89; p. 46-50, ISSN: 0014-4800, doi: 10.1016/j.yexmp.2010.05.001
• BONIN S., HLUBEK F., BENHATTAR J., DENKERT K., DIETEL M., FERNANDEZ P.L., HÖFLER G., KOTHMAIER H.,
KRUSLIN B., MAZZANTI C.M., PERREN A, POPPER H., SCARPA A., SOARES P., STANTA G., GROENEN P.J.T.A.
(2010). Multicentre validation study of nucleic acids extraction
from FFPE tissues. VIRCHOWS ARCHIV, vol. 457; p. 309-317, ISSN: 0945-6317, doi: 10.1007/s00428-010-0917-5
• DONADA M., BONIN S., NARDON E., DE PELLEGRIN A., DECORTI G., STANTA G. (2011). Thymidilate synthase
expression predicts longer survival in patients with stage II colon cancer treated with 5-flurouracil independently of
168
microsatellite instability. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, vol. 137; p. 201-210, ISSN:
0171-5216, doi: 10.1007/s00432-010-0872-1
• BONIN S., FILON LARESE F., TREVISAN G., AVIAN A., RUI F., STANTA G., BOVENZI M. (2011). Gene expression
changes in peripheral blood mononuclear cells in occupational exposure to nickel. EXPERIMENTAL DERMATOLOGY,
vol. 20; p. 147-148, ISSN: 0906-6705, doi: 10.1111/j.1600-0625.2010.01162.x
• DOTTI I., BONIN S., NARDON E. (2011). Reverse Transcription. In: Stanta G.. Guidelines for Molecular Analysis
in Archive Tissues. p. 93-97, Berlin Heidelberg: Springer-Verlag, ISBN/ISSN: 9783642178894, doi: 10.1007/9783-642-17890-0_19
• DOTTI I., NARDON E., PRACELLA D., BONIN S. (2011). Quantitative Real-Time RT-PCR. In: Stanta G.. Guidelines
for Molecular Analysis in Archive Tissues. p. 121-132, Berlin Heidelberg: Springer-Verlag, ISBN/ISSN:
9783642178894, doi: 10.1007/978-3-642-17890-0_25
• BASILI G., BONIN S., DOTTI I. (2011). DNA Sequencing from PCR Products. In: Stanta G.. Guidelines for
Molecular Analysis in Archive Tissues. p. 135-141, Berlin Heidelberg: Springer-Verlag, ISBN/ISSN:
9783642178894, doi: 10.1007/978-3-642-17890-0_26
• BONIN S., DOTTI I. (2011). Nested-PCR. In: Stanta G.. Guidelines for Molecular Analysis in Archive Tissues. p.
111-114, Berlin Heidelberg: Springer-Verlag, ISBN/ISSN: 9783642178894, doi: 10.1007/978-3-642-17890-0_22
• BONIN S., DOTTI I. (2011). General Protocol for End-Point PCR. In: Stanta G.. Guidelines for Molecular Analysis
in Archive Tissues. p. 101-103, Berlin Heidelberg: Springer-Verlag, ISBN/ISSN: 9783642178894, doi:
10.1007/978-3-642-17890-0_20
• DOTTI I, BONIN S. (2011). DNase Treatment of RNA. In: Stanta G.. Guidelines for Molecular Analysis in Archive
Tissues. p. 87-90, Berlin Heidelberg: Springer-Verlag, ISBN/ISSN: 9783642178894, doi: 10.1007/978-3-64217890-0_18
• BONIN S., DOTTI I. (2011). Quantitative End-Point PCR. In: Stanta G.. Guidelines for Molecular Analysis in
Archive Tissues. p. 105-109, Berlin Heidelberg: Springer-Verlag, ISBN/ISSN: 9783642178894, doi: 10.1007/9783-642-17890-0_21
• DOTTI I., BONIN S. (2011). Quantification of Nucleic Acids. In: Stanta G.. Guidelines for Molecular Analysis in
Archive Tissues. p. 75-79, Berlin Heidelberg: Springer-Verlag, ISBN/ISSN: 9783642178894, doi: 10.1007/978-3642-17890-0_16
• DOTTI I., BONIN S. (2011). Integrity Assessment of Nucleic Acids. In: Stanta G.. Guidelines for Molecular
Analysis in Archive Tissues. p. 81-85, Berlin Heidelberg: Springer-Verlag, ISBN/ISSN: 9783642178894, doi:
10.1007/978-3-642-17890-0_17
• BONIN S., STANTA G. (2011). RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues. In: Stanta G..
Guidelines for Molecular Analysis in Archive Tissues. p. 57-61, Berlin Heidelberg: Springer-Verlag, ISBN/ISSN:
9783642178894, doi: 10.1007/978-3-642-17890-0_12
• BONIN S., STANTA G. (2011). RNA Temperature Demodification. In: Stanta G.. Guidelines for Molecular Analysis
in Archive Tissues. p. 67-69, Berlin Heidelberg: Springer-Verlag, ISBN/ISSN: 9783642178894, doi: 10.1007/9783-642-17890-0_14
• BONIN S., TAVANO F. (2011). Restoration and Reconstruction of DNA Length. In: Stanta G.. Guidelines for
Molecular Analysis in Archive Tissues. p. 55-56, Berlin Heidelberg: Springer-Verlag Berlin Heidelberg, ISBN/ISSN:
9783642178894, doi: 10.1007/978-3-642-17890-0_11
• DOTTI I., BONIN S., BASILI G., FAORO V. (2011). Formalin-Free Fixatives. In: Stanta G.. Guidelines for
Molecular Analysis in Archive Tissues. p. 13-18, Berlin Heidelberg: Springer-Verlag, ISBN/ISSN: 9783642178894,
doi: 10.1007/978-3-642-17890-0_3
• BONIN S., GROENEN P.J.T.A., HALBWEDL I., POPPER H. (2011). DNA Extraction from Formalin-Fixed ParaffinEmbedded (FFPE) Tissues. In: Stanta G.. Guidelines for Molecular Analysis in Archive Tissues. p. 33-36, Berlin
Heidelberg: Springer-Verlag, ISBN/ISSN: 9783642178894, doi: 10.1007/978-3-642-17890-0_7
• BONIN S., GROENEN P.J.T.A., HALBWEDL I., POPPER H. (2011). DNA Extraction from Formalin-Fixed ParaffinEmbedded Tissues (FFPE) (from Small Fragments of Tissues or Microdissected Cells). In: Stanta G.. Guidelines for
Molecular Analysis in Archive Tissues. p. 37-39, Berlin Heidelberg: Springer-Verlag, ISBN/ISSN: 9783642178894,
doi: 10.1007/978-3-642-17890-0_8
• STANTA G., BONIN S., MACHADO I., LLOMBART-BOSCH A. (2011). Models of Biobanking and Tissue
Preservation: RNA Quality in Archival Samples in Pathology Laboratories and “In Vivo Biobanking” by Tumor
Xenografts in Nude Mice—Two Models of Quality Assurance in Pathology. BIOPRESERVATION AND BIOBANKING,
vol. 9; p. 149-155, ISSN: 1947-5543
3. BRESCHI Lorenzo
• SUPPA P, RUGGERI A JR, TAY FR, PRATI C, BIASOTTO M, FALCONI M, PASHLEY DH, BRESCHI L. (2006).
Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin. JOURNAL OF DENTAL RESEARCH, vol.
85; p. 133-137, ISSN: 0022-0345
• TAY FR, MAZZONI A, PASHLEY DH, DAY TE, NGOH EC, BRESCHI L. (2006). Potential iatrogenic tetracycline
staining of endodontically treated teeth via NaOCl/MTAD irrigation – a preliminary report. JOURNAL OF
ENDODONTICS, vol. 32; p. 354-358, ISSN: 0099-2399
• YIU CKY, HIRAISHI N, CHERSONI S, BRESCHI L., FERRARI M, PRATI C, KING NNM, PASHLEY DH, TAY FR (2006).
Single-bottle adhesives behave as permeable membranes after polymerisation. II. Differential permeability
reduction with oxalate desensitiser. JOURNAL OF DENTISTRY, vol. 34; p. 106-116, ISSN: 0300-5712
• SAURO S, WATSON TF, TAY FR, CHERSONI S, BRESCHI L., BERNARDI F, PRATI C (2006). Water uptake of
bonding systems applied on root dentin surfaces: SEM and confocal microscopic study. DENTAL MATERIALS, vol.
22; p. 671-680, ISSN: 0109-5641
• VENTURI M, DI LENARDA R., BRESCHI L. (2006). AN EX VIVO COMPARISON OF THREE DIFFERENT GUTTAPERCHA CONES WHEN COMPACTED AT DIFFERENT TEMPERATURES: RHEOLOGICAL CONSIDERATIONS IN
RELATION TO THE FILLING OF LATERAL CANALS. INTERNATIONAL ENDODONTIC JOURNAL, vol. 39(8); p. 648656, ISSN: 0143-2885, doi: 10.1111/J.1365-2591.2006.01133.X
• MAZZONI A, PASHLEY DH, NISHITANI Y, BRESCHI L., TJADERHANE L, TOLEDANO M, PASHLEY EL, TAY FR
169
(2006). Reactivation of quenched endogenous proteolytic activities in phosphoric acid-etched dentine by etch-andrinse adhesives. BIOMATERIALS, vol. 27; p. 4470-4476, ISSN: 0142-9612
• CADENARO M., BRESCHI L., ANTONIOLLI F, MAZZONI A, DI LENARDA R (2006). Influence of whitening on the
degree of conversion of dental adhesives on dentin. EUROPEAN JOURNAL OF ORAL SCIENCES, vol. 114(3); p. 257262, ISSN: 0909-8836
• NISHITANI Y, YOSHIYAMA M, WADGAONKAR B, BRESCHI L., MANNELLO N, MAZZONI A, CARVALHO R,
TJADERHANE L, TAY FR, PASHLEY DH (2006). Activation of gelatinolytic/collagenolytic activity in dentin by selfetching adhesives. EUROPEAN JOURNAL OF ORAL SCIENCES, vol. 114; p. 160-166, ISSN: 0909-8836
• PASQUANTONIO G, TAY FR, MAZZONI A, SUPPA P, RUGGERI A JR, FALCONI M, DI LENARDA R, BRESCHI L.
(2007). Electric device improves bonds of simplified etch-and-rinse adhesives. DENTAL MATERIALS, vol. 23; p.
513-518, ISSN: 0109-5641
• BRESCHI L., MAZZONI A, PASHLEY DH, PASQUANTONIO G, RUGGERI A, SUPPA P, MAZZOTTI G, DI LENARDA R.,
TAY FR (2006). Electric-current-assisted Application of Self-etch Adhesives to Dentin. JOURNAL OF DENTAL
RESEARCH, vol. 85; p. 1092-1096, ISSN: 0022-0345
• HIRAISHI N, BRESCHI L., PRATI C, FERRARI M, TAGAMI J, KING NM (2007). Technique sensitivity associated
with air-drying of HEMA-free, single-bottle, one-step self-etch adhesives. DENTAL MATERIALS, vol. 23; p. 498-505,
ISSN: 0109-5641
• RUGGERI A JR, PRATI C, MAZZONI A, NUCCI C, DI LENARDA R., MAZZOTTI G, BRESCHI L., L (2007). Effects of
citric acid and EDTA conditioning on exposed root dentin: An
immunohistochemical analysis of collagen and proteoglycans. ARCHIVES OF ORAL BIOLOGY, vol. 52; p. 1-8, ISSN:
0003-9969
• VENTURI M, BRESCHI L. (2007). A comparison between two electronic apex locators: an ex-vivo investigation.
INTERNATIONAL ENDODONTIC JOURNAL, vol. 40; p. 362-373, ISSN: 0143-2885
• BRESCHI L., CADENARO M., ANTONIOLLI F, SAURO S, BIASOTTO M, PRATI C, TAY FR, DI LENARDA R (2007).
Polymerization kinetics of dental adhesives cured with LED: correlation between extent of conversion and
permeability. DENTAL MATERIALS, vol. 23(9); p. 1066-1072, ISSN: 0109-5641
• FALCONI M, TETI G, ZAGO M, PELOTTI S, GOBBI P, BRESCHI L., MAZZOTTI G (2007). Effect of fixative on
chromatin structure and DNA detection. MICROSCOPY RESEARCH AND TECHNIQUE, vol. 70; p. 599-606, ISSN:
1059-910X
• BRESCHI L., MAZZONI A, RUGGERI A, CADENARO M, DI LENARDA R., DE STEFANO DORIGO E (2008). Dental
adhesion review: Aging and stability of the bonded interface. DENTAL MATERIALS, vol. 24; p. 90-101, ISSN: 01095641
• MAZZONI A, MANNELLO F, TAY FR, TONTI GAM, PAPA S, MAZZOTTI G, DI LENARDA R, PASHLEY DH, BRESCHI L.
(2007). Zymographic analysis and characterization of MMP-2 and -9 forms in human sound dentin. JOURNAL OF
DENTAL RESEARCH, vol. 86; p. 436-440, ISSN: 0022-0345
• FALCONI M, TETI G, ZAGO M, PELOTTI S, BRESCHI L., MAZZOTTI G (2007). Effects of HEMA on type I collagen
protein in human gingival fibroblasts. CELL BIOLOGY AND TOXICOLOGY, vol. 23; p. 313-322, ISSN: 0742-2091
• ORSINI G, RUGGERI A JR, MAZZONI A, PAPA V, MAZZOTTI G, DI LENARDA R., BRESCHI L. (2007).
Immunohistochemical identification of decorin and biglycan in human dentin: a correlative field emission scanning
electron microscopy/transmission electron microscopy study. CALCIFIED TISSUE INTERNATIONAL, vol. 81; p. 3945, ISSN: 0171-967X
• CARRILHO MRO, GERALDELI S, TAY FR, DE GOES M, CARVALHO RM, TJDERHANE L, REIS AF, HEBLING J,
MAZZONI A, BRESCHI L., PASHLEY DH (2007). In Vivo Preservation of Hybrid Layer by Chlorhexidine. JOURNAL OF
• BERTACCI A, BARONI C, BRESCHI L., VENTURI M, PRATI C (2007). The influence of smear layer in lateral
channels filling. CLINICAL ORAL INVESTIGATIONS, vol. 11; p. 353-359, ISSN: 1432-6981
• ORSINI G, RUGGERI A JR, MAZZONI A, PAPA V, PICCIRILLI M, FALCONI M, DI LENARDA R, BRESCHI L. (2007).
Immunohistochemical identification of type I and type III collagen and chondroitin sulphate in human predentine: a
correlative FEI-SEM/TEM study. INTERNATIONAL ENDODONTIC JOURNAL, vol. 40; p. 669-678, ISSN: 0143-2885
• BRESCHI L., CADENARO M., ANTONIOLLI F, VISINTINI E, TOLEDANO M, DI LENARDA R (2007). Extent of
polymerization of dental bonding systems on bleached enamel. AMERICAN JOURNAL OF DENTISTRY, vol. 20(4); p.
275-280, ISSN: 0894-8275
• FERRARI M, CONIGLIO I, MAGNI E, CAGIDIACO MC, GALLINA G, PRATI C, BRESCHI L. (2008). How can droplets
formation occur in endodontically treated teeth during bonding procedures?. JOURNAL OF ADHESIVE DENTISTRY,
vol. 10; p. 211-218, ISSN: 1461-5185
• CADENARO M., BRESCHI L., NUCCI C, ANTONIOLLI F, VISINTINI E, PRATI C, MATIS BA, DI LENARDA R (2008).
Effect of two in-office whitening agents on enamel surface in vivo: a morphological and non contact profilometric
study. OPERATIVE DENTISTRY, vol. 33; p. 123-130, ISSN: 0361-7734
• CONTARDO L, DE LUCA M, BEVILACQUA L., BRESCHI L., DI LENARDA R (2007). Influence of calcium hydroxide
debris on the quality of endodontic apical seal. MINERVA STOMATOLOGICA, vol. 56(10); p. 509-517, ISSN: 00264970
• CADENARO M, BIASOTTO M., SCUOR N, BRESCHI L., DAVIDSON CL, DI LENARDA R (2008). Assessment of
polymerization contraction stress of three composite resins. DENTAL MATERIALS, vol. 24 (5); p. 681-685, ISSN:
0109-5641, doi: 10.1016/J.DENTAL.2007.06.031
• CADENARO M., BRESCHI L., ANTONIOLLI F, NAVARRA CO, MAZZONI A, TAY FR, DI LENARDA R, PASHLEY DH
(2008). Degree of conversion of resin blends in relation to ethanol content and hydrophilicity. DENTAL MATERIALS,
vol. 24; p. 1194-1200, ISSN: 0109-5641
• MAZZONI A, PASHLEY DH, TAY FR, GOBBI P, ORSINI G, RUGGERI A JR, CARRILHO M, TJÄDERHANE L, DI
LENARDA R., BRESCHI L. (2009). Immunohistochemical identification of MMP-2 and MMP-9 in human dentin:
Correlative FEI-SEM/TEM analysis. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A, vol. 88; p. 697-703,
ISSN: 1549-3296
• ZAGO M, TETI G, MAZZOTTI G, RUGGERI A JR, BRESCHI L., PELOTTI S, ORTOLANI M, FALCONI M (2008).
Expression of procollagen α1 type I and tenascin proteins induced by HEMA in human pulp fibroblasts.
170
TOXICOLOGY IN VITRO, vol. 22; p. 1153-1159, ISSN: 0887-2333
• STACCHI C, ORSINI G, DI IORIO D, BRESCHI L., DI LENARDA R (2008). Clinical, histologic and
histomorphometric analyses of regenerated bone in maxillary sinus augmentation using fresh frozen human bone
allografts. JOURNAL OF PERIODONTOLOGY, vol. 79; p. 1789-1796, ISSN: 0022-3492
• SAVARINO L, GRECO M, BALDINI N, GIUNTI A, PISTONE M, MARCHIONNI S, BRESCHI L., PRATI C (2008).
Evaluation of restorative materials with a new perfusion system. JOURNAL OF ADHESIVE DENTISTRY, vol. 10; p.
269-275, ISSN: 1461-5185
• VISINTINI E, MAZZONI A, VITA F, PASQUANTONIO G, CADENARO M., DI LENARDA R, BRESCHI L. (2008).
Effects of thermocycling and use of ElectroBond on microtensile strength and nanoleakage using commercial onestep self-etch adhesives. EUROPEAN JOURNAL OF ORAL SCIENCES, vol. 116(6); p. 564-570, ISSN: 0909-8836,
doi: DOI: 10.1111/J.1600-0722.2008.00576.X
• ORSINI G, RUGGERI A JR, MAZZONI A, NATO A, FALCONI M, DI LENARDA R., NANCI A, BRESCHI L. (2008).
Immunohistochemical Localization of DMP1 in Human Dentin. EUROPEAN JOURNAL OF HISTOCHEMISTRY, vol. 52;
p. 215-220, ISSN: 1121-760X
• SABOIA VPA, NATO F, MAZZONI A, ORSINI G, PUTIGNANO A, GIANNINI M, BRESCHI L. (2008). Adhesion of a
two-step etch-and-rinse adhesive on collagen-depleted dentin. JOURNAL OF ADHESIVE DENTISTRY, vol. 10; p.
419-422, ISSN: 1461-5185
• TETI G, MAZZOTTI G, ZAGO M, ORTOLANI M, BRESCHI L., PELOTTI S, RUGGERI A, FALCONI M (2009). HEMA
down-regulates procollagen α1 type I in human gingival fibroblasts. JOURNAL OF BIOMEDICAL MATERIALS
RESEARCH. PART A, vol. 90; p. 256-262, ISSN: 1549-3296
• CADENARO M, BRESCHI L., RUEGGEBERG FA, AGEE K, DI LENARDA R., CARRILHO M, TAY FR, PASHLEY DH
(2009). Effect of adhesive hydrophilicity and curing-time on the permeability of resins bonded to water vs. ethanolsaturated acid-etched dentin. DENTAL MATERIALS, vol. 25; p. 39-47, ISSN: 0109-5641
• CARRILHO MR, TAY FR, DONNELLY AD, AGEE KA, TJÄDERHANE L, MAZZONI A, BRESCHI L., FOULGER S,
PASHLEY DH (2009). Host-derived loss of dentin matrix stiffness associated with solubilization of collagen.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART B, APPLIED BIOMATERIALS, vol. 90; p. 373-380, ISSN:
1552-4981
• CARVALHO CA, CANTORO A, MAZZONI A, GORACCI C, BRESCHI L., FERRARI M (2009). Effect of the ethanol
application on post luting to intraradicular dentin. INTERNATIONAL ENDODONTIC JOURNAL, vol. 42; p. 129-135,
ISSN: 0143-2885
• MAZZONI A, VISINTINI E, VITA F, PASQUANTONIO G, SABOIA VP, RUGGERI A JR, DI LENARDA R, DE STEFANO
DORIGO E., BRESCHI L. (2009). ElectroBond improves immediate dentin microtensile bond strength of two etchand-rinse adhesives. JOURNAL OF ADHESIVE DENTISTRY, vol. 11(1); p. 27-33, ISSN: 1461-5185, doi:
DOI:10.3290/J.JAD.A14729
• BRESCHI L., CAMMELLI F, VISINTINI E, MAZZONI A, VITA F, CARRILHO M, CADENARO M., FOULGER S,
MAZZOTI G, TAY FR, DI LENARDA R, PASHLEY D (2009). Influence of chlorhexidine concentration on the durability
of etch-and-rinse dentin bonds: a 12-month in vitro study. JOURNAL OF ADHESIVE DENTISTRY, vol. 11; p. 191198, ISSN: 1461-5185
• BRESCHI L., MAZZONI A, DE STEFANO DORIGO E, FERRARI M (2009). Adhesion to intraradicular dentin: a
review. JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY, vol. 23; p. 1053-1083, ISSN: 0169-4243
• ERHARDT ACG, OSORIO R, PROENÇA JP, AGUILERA FA, OSORIO E, BRESCHI L., TOLEDANO M (2009). Influence
of multiple adhesive coatings and extended etching time on the bonding effectiveness of self-etch adhesives to
dentin. OPERATIVE DENTISTRY, ISSN: 0361-7734
• SANTOS J, CARRILHO MR, TERVAHARTIALA T, SORSA T, FERRAZ C, BRESCHI L., MAZZONI A, PASHLEY DH,
TJÄDERHANE L (2009). Determination of matrix metalloproteinases in human radicular dentin. JOURNAL OF
ENDODONTICS, vol. 35; p. 686-689, ISSN: 0099-2399
• CADENARO M., BRESCHI L., RUEGGEBERG FA, SUCHKO M, GRODIN E, AGEE K, DI LENARDA R, TAY FR,
PASHLEY DH (2009). Effects of residual ethanol on the rate and degree of conversion of five experimental resins.
DENTAL MATERIALS, vol. 25(5); p. 621-628, ISSN: 0109-5641, doi: 10.1016/J.DENTAL.2008.11.005
• CADENARO M., MARCHESI G, ANTONIOLLI F, DAVIDSON C, DE STEFANO DORIGO E, BRESCHI L. (2009).
Flowability of composites is no guarantee for contraction stress reduction. DENTAL MATERIALS, vol. 25(5); p. 649654, ISSN: 0109-5641, doi: 10.1016/J.DENTAL.2008.11.010
• CARVALHO CA, MONTICELLI F, CANTORO A, BRESCHI L., FERRARI M (2009). Push-out bond strength of fiber
posts luted with unfilled resin cement. JOURNAL OF ADHESIVE DENTISTRY, vol. 11; p. 65-70, ISSN: 1461-5185
• KOMORI PC, PASHLEY DH, TJADERHANE L, BRESCHI L., MAZZONI A, DE GOES MF, WANG L, CARRILHO MR
(2009). Effect of 2% chlorhexidine digluconate on the bond strength to normal vs caries-affected dentin.
OPERATIVE DENTISTRY, vol. 43; p. 157-165, ISSN: 0361-7734
• FALCONI M, TETI G, ZAGO M, GALANZI A, BRESCHI L., PELOTTI S, RUGGERI A, MAZZOTTI G (2009). Influence
of a commercial tattoo ink on protein production in human fibroblasts. ARCHIVES OF DERMATOLOGICAL
• MAI S, KIM YK, TOLEDANO M, BRESCHI L., LING JQ, PASHLEY DH, TAY FR (2009). Phosphoric acid esters cannot
replace polyvinylphosphonic acid as phosphoprotein analogs in biomimetic remineralization of resin-bonded dentin.
DENTAL MATERIALS, vol. 25; p. 1230-1239, ISSN: 0109-5641
• CADENARO M, PASHLEY DH, MARCHESI G, CARRILHO M, ANTONIOLLI F, MAZZONI A, TAY FR, DI LENARDA R,
BRESCHI L. (2009). Influence of chlorhexidine on the degree of conversion and E-modulus of experimental
adhesive blends. DENTAL MATERIALS, vol. 25(10); p. 1269-1274, ISSN: 0109-5641, doi:
10.1016/J.DENTAL.2009.05.008
• NAVARRA C.O, CADENARO M, ARMSTRONG S.R, JESSOP J, ANTONIOLLI F, SERGO V., DI LENARDA R, BRESCHI
L. (2009). Degree of conversion of Filtek Silorane Adhesive System and Clearfil SE Bond within the hybrid and
adhesive layer: An in situ Raman analysis. DENTAL MATERIALS, vol. 25(9); p. 1178-1185, ISSN: 0109-5641, doi:
10.1016/J.DENTAL.2009.05.009
• MALACARNE-ZANON J, PASHLEY DH, AGEE KA, FOULGER S, ALVES MC, BRESCHI L., CADENARO M, GARCIA FP,
CARRILHO MR (2009). Effects of ethanol addition on the water sorption/solubility and percent conversion of
171
comonomers in model dental adhesives. DENTAL MATERIALS, vol. 25(10); p. 1275-1284, ISSN: 0109-5641, doi:
10.1016/J.DENTAL.2009.03.015
• MAZZONI A, MARCHESI G, CADENARO M, MAZZOTTI G, DI LENARDA R, FERRARI M, BRESCHI L. (2009). Pushout stress for fibre posts luted using different adhesive strategies. EUROPEAN JOURNAL OF ORAL SCIENCES, vol.
117; p. 447-453, ISSN: 0909-8836
• NAVARRA C.O, CADENARO M, CODAN B, MAZZONI A, SERGO V., DE STEFANO DORIGO E, BRESCHI L. (2009).
Degree of conversion and interfacial nanoleakage expression of three one-step self-etch adhesives. EUROPEAN
JOURNAL OF ORAL SCIENCES, vol. 117; p. 463-469, ISSN: 0909-8836, doi: 10.1111/j.1600-0722.2009.00654.x
• CADENARO M., DELISE C, ANTONIOLLI F, NAVARRA CO, DI LENARDA R, BRESCHI L. (2009). Enamel and dentin
bond strength following gaseous ozone application. JOURNAL OF ADHESIVE DENTISTRY, vol. 11; p. 287-292,
ISSN: 1461-5185
• SABOIA VP, SILVA FC, NATO F, MAZZONI A, CADENARO M., MAZZOTTI G, GIANNINI M, BRESCHI L. (2009).
Analysis of differential artificial ageing of the adhesive interface produced by a two-step etch-and-rinse adhesive.
EUROPEAN JOURNAL OF ORAL SCIENCES, vol. 117; p. 618-624, ISSN: 0909-8836
• FERRARI M, CARVALHO CA, GORACCI C, ANTONIOLLI F, MAZZONI A, MAZZOTTI G, CADENARO M, BRESCHI L.
(2009). Influence of luting material filler content on post cementation. JOURNAL OF DENTAL RESEARCH, vol. 88; p.
951-956, ISSN: 0022-0345
• RUGGERI A, ORSINI G, MAZZONI A, NATO F, PAPA V, PICCIRILLI M, PUTIGNANO A, MAZZOTTI G, DE STEFANO
DORIGO E, BRESCHI L. (2009). Immunohistochemical and biochemical assay of versican in human sound
predentine/dentine matrix. EUROPEAN JOURNAL OF HISTOCHEMISTRY, vol. 53(3); p. 125-133, ISSN: 1121-760X
• MAZZONI A, PASHLEY DH, RUGGERI A JR, VITA F, FALCONI M, DI LENARDA R, BRESCHI L. (2008). Adhesion to
chondroitinase ABC treated dentin. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART B, APPLIED
BIOMATERIALS, vol. 86B; p. 228-236, ISSN: 1552-4981
• PARAGLIOLA R., FRANCO V., FABIANI C., MAZZONI A., NATO F., TAY F.R., BRESCHI L., GRANDINI S. (2010).
Final rinse optimization: influence of different agitation protocols. JOURNAL OF ENDODONTICS, vol. 36; p. 282285, ISSN: 0099-2399
• SADEK F.T., MAZZONI A., BRESCHI L., TAY F.R., BRAGA R.R. (2010). Six-month evaluation of adhesives
interface created by a hydrophobic adhesive to acid-etched ethanol-wet bonded dentine with simplified dehydration
protocols. JOURNAL OF DENTISTRY, vol. 38; p. 276-283, ISSN: 0300-5712
• BRESCHI L., MAZZONI A., NATO F., CARRILHO M., VISINTINI E., TJÄDERHANE L., RUGGERI A. JR, TAY F.R., DE
STEFANO DORIGO E., PASHLEY D.H. (2010). Chlorhexidine stabilizes the adhesive interface: a 2 year in vitro
study. DENTAL MATERIALS, vol. 26; p. 320-325, ISSN: 0109-5641
• KIM J., GU L., BRESCHI L., TJÄDERHANE L., CHOI K.K., PASHLEY D.H., TAY F.R. (2010). Implication of Ethanol
Wet-bonding in Hybrid Layer Remineralization. JOURNAL OF DENTAL RESEARCH, vol. 89; p. 575-580, ISSN: 00220345
• TASCHNER M, NATO F, MAZZONI A, FRANKENBERGER R, KRÄMER N, DI LENARDA R, PETSCHELT A, BRESCHI L.
(2010). Role of preliminary etching for one-step self-etch adhesives. EUROPEAN JOURNAL OF ORAL SCIENCES, vol.
118; p. 517-524, ISSN: 0909-8836, doi: 10.1111/j.1600-0722.2010.00769.x.
• KIM J, UCHIYAMA T, CARRILHO M, AGEE KA, MAZZONI A, BRESCHI L., CARVALHO RM, TJÄDERHANE L, LOONEY
S, WIMMER C, TEZVERGIL-MUTLUAY A, TAY FR, PASHLEY DH. (2010). Chlorhexidine binding to mineralized versus
demineralized dentin powder. DENTAL MATERIALS, vol. 26; p. 771-778, ISSN: 0109-5641
• CARRILHO MR, CARVALHO RM, SOUSA EN, NICOLAU J, BRESCHI L., MAZZONI A, TJÄDERHANE L, TAY FR, AGEE
K, PASHLEY DH. (2010). Substantivity of chlorhexidine to human dentin. DENTAL MATERIALS, vol. 26; p. 779-785,
ISSN: 0109-5641
• MARCHESI G, BRESCHI L., ANTONIOLLI F, DI LENARDA R, FERRACANE J, CADENARO M. (2010). Contraction
stress of low-shrinkage composite materials assessed with different testing systems. DENTAL MATERIALS, vol. 26;
p. 947-953, ISSN: 0109-5641
• KIM YK, GU LS, BRYAN TE, KIM JR, CHEN L, LIU Y, YOON JC, BRESCHI L., PASHLEY DH, TAY FR (2010).
Mineralisation of reconstituted collagen using polyvinylphosphonic acid/polyacrylic acid templating matrix protein
analogues in the presence of calcium, phosphate and hydroxyl ions. BIOMATERIALS, vol. 31; p. 6618-6627, ISSN:
0142-9612
• TEZVERGIL-MUTLUAY A, AGEE KA, HOSHIKA T, CARRILHO M, BRESCHI L., TJÄDERHANE L, NISHITANI Y,
CARVALHO RM, LOONEY S, TAY FR, PASHLEY DH. (2010). The requirement of zinc and calcium ions for functional
MMP activity in demineralized dentin matrices. DENTAL MATERIALS, vol. 26; p. 1059-1067, ISSN: 0109-5641
4. CADENARO Milena
• CADENARO M., BRESCHI L, ANTONIOLLI F, VISINTINI E, DORIGO E, DI LENARDA R. (2006). Effetto delle
lampade LED sulla microdurezza degli adesivi smalto-dentinali. GIORNALE ITALIANO DI CONSERVATIVA, ISSN:
1724-2908
• BRESCHI L, CADENARO M., ANTONIOLLI F, VISINTINI E, TOLEDANO M, DI LENARDA R (2007). Extent of
275-280, ISSN: 0894-8275
• CADENARO M., BIASOTTO M., SCUOR N, BRESCHI L, DAVIDSON CL, DI LENARDA R (2008). Assessment of
0109-5641, doi: 10.1016/J.DENTAL.2007.06.031
• BEVILACQUA L, CADENARO M., SOSSI A, BIASOTTO M, DI LENARDA R (2007). Influence of air abrasion and
etching on enamel and adaptation of a dental sealant. EUROPEAN JOURNAL OF PAEDIATRIC DENTISTRY, vol. 8(1);
p. 25-30, ISSN: 1591-996X
• BRESCHI L, CADENARO M., ANTONIOLLI F, SAURO S, BIASOTTO M, PRATI C, TAY FR, DI LENARDA R (2007).
• CADENARO M., BRESCHI L, ANTONIOLLI F, MAZZONI A, DI LENARDA R (2006). Influence of whitening on the
degree of conversion of dental adhesives on dentin. EUROPEAN JOURNAL OF ORAL SCIENCES, vol. 114(3); p. 257-
172
262, ISSN: 0909-8836
• CADENARO M., BIASOTTO M, CONTARDO L, CHIESA R, DI LENARDA R, DORIGO E (2006). Surface roughness of
three resin restorative materials after finishing and polishing. MINERVA STOMATOLOGICA, vol. 55(4); p. 179-187,
ISSN: 0026-4970
• BEVILACQUA L., SOSSI A, CADENARO M., DI LENARDA R (2007). Comparative evaluation of the microhardness
of 4 dental sealants. EUROPEAN JOURNAL OF PAEDIATRIC DENTISTRY, vol. 8 (4); p. 179-182, ISSN: 1591-996X
• CADENARO M., BRESCHI L, NUCCI C, ANTONIOLLI F, VISINTINI E, PRATI C, MATIS BA, DI LENARDA R (2008).
• BRESCHI L, MAZZONI A, RUGGERI A, CADENARO M., DI LENARDA R., DE STEFANO DORIGO E (2008). Dental
• VISINTINI E, MAZZONI A, VITA F, PASQUANTONIO G, CADENARO M., DI LENARDA R, BRESCHI L (2008). Effects
of thermocycling and use of ElectroBond on microtensile strength and nanoleakage using commercial one-step selfetch adhesives. EUROPEAN JOURNAL OF ORAL SCIENCES, vol. 116(6); p. 564-570, ISSN: 0909-8836, doi: DOI:
10.1111/J.1600-0722.2008.00576.X
• CADENARO M., BRESCHI L, RUEGGEBERG FA, AGEE K, DI LENARDA R., CARRILHO M, TAY FR, PASHLEY DH
• CADENARO M., BRESCHI L, ANTONIOLLI F, NAVARRA CO, MAZZONI A, TAY FR, DI LENARDA R, PASHLEY DH
vol. 24; p. 1194-1200, ISSN: 0109-5641
• CADENARO M., MARCHESI G, ANTONIOLLI F, DAVIDSON C, DE STEFANO DORIGO E, BRESCHI L (2009).
Flowability of composites is no guarantee for contraction stress reduction. DENTAL MATERIALS, vol. 25(5); p. 649654, ISSN: 0109-5641, doi: 10.1016/J.DENTAL.2008.11.010
• CADENARO M., BRESCHI L, RUEGGEBERG FA, SUCHKO M, GRODIN E, AGEE K, DI LENARDA R, TAY FR, PASHLEY
DH (2009). Effects of residual ethanol on the rate and degree of conversion of five experimental resins. DENTAL
MATERIALS, vol. 25(5); p. 621-628, ISSN: 0109-5641, doi: 10.1016/J.DENTAL.2008.11.005
• CADENARO M., DELISE C, ANTONIOLLI F, NAVARRA CO, DI LENARDA R, BRESCHI L (2009). Enamel and dentin
ISSN: 1461-5185
• SABOIA VP, SILVA FC, NATO F, MAZZONI A, CADENARO M., MAZZOTTI G, GIANNINI M, BRESCHI L (2009).
Analysis of differential artificial ageing of the adhesive interface produced by a two-step etch-and-rinse adhesive.
• FERRARI M, CARVALHO CA, GORACCI C, ANTONIOLLI F, MAZZONI A, MAZZOTTI G, CADENARO M., BRESCHI L.
(2009). Influence of luting material filler content on post cementation. JOURNAL OF DENTAL RESEARCH, vol. 88; p.
951-956, ISSN: 0022-0345
• NAVARRA C.O, CADENARO M., CODAN B, MAZZONI A, SERGO V., DE STEFANO DORIGO E, BRESCHI L (2009).
• MAZZONI A, MARCHESI G, CADENARO M., MAZZOTTI G, DI LENARDA R, FERRARI M, BRESCHI L. (2009). Pushout stress for fibre posts luted using different adhesive strategies. EUROPEAN JOURNAL OF ORAL SCIENCES, vol.
117; p. 447-453, ISSN: 0909-8836
• BRESCHI L, CAMMELLI F, VISINTINI E, MAZZONI A, VITA F, CARRILHO M, CADENARO M., FOULGER S, MAZZOTI
G, TAY FR, DI LENARDA R, PASHLEY D (2009). Influence of chlorhexidine concentration on the durability of etchand-rinse dentin bonds: a 12-month in vitro study. JOURNAL OF ADHESIVE DENTISTRY, vol. 11; p. 191-198,
ISSN: 1461-5185
• MALACARNE-ZANON J, PASHLEY DH, AGEE KA, FOULGER S, ALVES MC, BRESCHI L., CADENARO M., GARCIA FP,
CARRILHO MR (2009). Effects of ethanol addition on the water sorption/solubility and percent conversion of
comonomers in model dental adhesives. DENTAL MATERIALS, vol. 25(10); p. 1275-1284, ISSN: 0109-5641, doi:
10.1016/J.DENTAL.2009.03.015
• NAVARRA C.O, CADENARO M., ARMSTRONG S.R, JESSOP J, ANTONIOLLI F, SERGO V., DI LENARDA R, BRESCHI
L (2009). Degree of conversion of Filtek Silorane Adhesive System and Clearfil SE Bond within the hybrid and
10.1016/J.DENTAL.2009.05.009
• CADENARO M., PASHLEY DH, MARCHESI G, CARRILHO M, ANTONIOLLI F, MAZZONI A, TAY FR, DI LENARDA R,
10.1016/J.DENTAL.2009.05.008
• BRESCHI L, CADENARO M., MAZZONI L, NAVARRA CO, MARCHESI G, DI LENARDA R, DE STEFANO DORIGO E
(2009). La durata del legame adesivo: la nuova sfida dell’odontoiatria restaurativa. IL DENTISTA MODERNO, vol.
4; p. 41-52, ISSN: 1827-3726
• CADENARO M., NAVARRA C.O., ANTONIOLLI F., MAZZONI A., DI LENARDA R., RUEGGEBERG F.A., BRESCHI L.
(2010). The effect of curing mode on extent of polymerization and microhardness of dual-cured, self-adhesive resin
cements. AMERICAN JOURNAL OF DENTISTRY, vol. 23; p. 14-18, ISSN: 0894-8275
• CADENARO M., NAVARRA C.O., MAZZONI A., NUCCI C., MATIS B.A., DI LENARDA R., BRESCHI L. (2010). An in
vivo study of the effect of a 38 percent hydrogen peroxide in-office whitening agent on enamel. THE JOURNAL OF
THE AMERICAN DENTAL ASSOCIATION, vol. 141; p. 449-454, ISSN: 0002-8177
• BRESCHI L., MARTIN P., MAZZONI A., NATO F., CARRILHO M., TJÄDERHANE L., VISINTINI E., CADENARO M.,
TAY F.R., DORIGO E.D., PASHLEY D.H. (2010). Use of a specific MMP-inhibitor (galardin) for preservation of hybrid
layer. DENTAL MATERIALS, vol. 26; p. 571-578, ISSN: 0109-5641
• CADENARO M., ANTONIOLLI F., CODAN B., AGEE K., TAY F.R., DE STEFANO DORIGO E., PASHLEY D.H.,
BRESCHI L. (2010). Influence of different initiators on the degree of conversion of experimental adhesive blends in
173
relation to their hydrophilicity and solvent content. DENTAL MATERIALS, vol. 26; p. 288-294, ISSN: 0109-5641
• ZHANG K, KIM Y.K., CADENARO M., BRYAN T.E., SIDOW S.J., LOUSHINE R.J., LING J.Q., PASHLEY D.H., TAY
F.R. (2010). Effects of different exposure times and concentrations of NaOCl/EDTA on the structural integrity of
mineralized dentin. JOURNAL OF ENDODONTICS; p. 105-109, ISSN: 0099-2399
• MARCHESI G, BRESCHI L, ANTONIOLLI F, DI LENARDA R, FERRACANE J, CADENARO M. (2010). Contraction
p. 947-953, ISSN: 0109-5641
• TEZVERGIL-MUTLUAY A, AGEE KA, UCHIYAMA T, IMAZATO S, MUTLUAY MM, CADENARO M., BRESCHI L,
NISHITANI Y, TAY FR, PASHLEY DH. (2011). The Inhibitory Effects of Quaternary Ammonium Methacrylates on
Soluble and Matrix-bound MMPs. JOURNAL OF DENTAL RESEARCH, vol. 90; p. 535-540, ISSN: 0022-0345
• CADENARO M., CODAN B., NAVARRA C.O., MARCHESI G., TURCO G., DI LENARDA R., BRESCHI L. (2011).
Contraction stress, elastic modulus, and degree of conversion of three flowable composites. EUROPEAN JOURNAL
OF ORAL SCIENCES, vol. 119; p. 241-245, ISSN: 0909-8836
5. CESARO Attilio
• DONATI I, CESARO A., PAOLETTI S. (2006). Specific Interactions versus Counterion Condensation. 1. Nongelling
Ions/Polyuronate Systems. BIOMACROMOLECULES, vol. 7(1); p. 281-287, ISSN: 1525-7797, doi:
10.1021/BM050646P
• LEFORT R, BORDAT P, CESARO A., DESCAMPS M (2007). Exploring conformational energy landscape of glassy
disaccharides by cross polarization magic angle spinning 13C NMR and numerical simulations. I. Methodological
aspects. THE JOURNAL OF CHEMICAL PHYSICS, vol. 126; p. 014510-1-014510-9, ISSN: 0021-9606
• LEFORT R, BORDAT P, CESARO A., DESCAMPS M (2007). CESARO A. (2007). Exploring conformational energy
landscape of glassy disaccharides by cross polarization magic angle spinning 13C NMR and numerical simulations.
II. Enhanced molecular flexibility in amorphous trehalose. THE JOURNAL OF CHEMICAL PHYSICS, vol. 126; p.
014511-1-014511-9, ISSN: 0021-9606
• CESARO A. (2006). Carbohydrates - All dried up. NATURE MATERIALS, vol. 5; p. 593-594, ISSN: 1476-1122
• DE GIACOMO O, CESARO A., QUARONI L (2006). IR MICROSCOPY OF BIOPOLYMERS UNDERGOING
PHASE SEPARATION AT THE SISSI BEAMLINE. In: BILLE E., ET AL. Elettra Research Highlight. p. 31-33, TRIESTE:
Elettra
• DONATI I, BENEGAS JC, CESARO A., PAOLETTI S. (2006). Specific interactions versus counterion condensation.
2. Theoretical treatment within the counterion condensation theory. BIOMACROMOLECULES, vol. 7 (5); p. 15871596, ISSN: 1525-7797, doi: 10.1021/bm050981d
• CESARO A., GAMINI A, DE GIACOMO O, MASCIOVECCHIO C, GESSINI A, DI FONZO S (2007). Study of
longitudinal dynamics of trehalose-water solutions by inelastic ultraviolet scattering. PHILOSOPHICAL MAGAZINE,
vol. 87; p. 623-630, ISSN: 1478-6435
• DONATI I., BORGOGNA M, TURELLO E, CESARO A., PAOLETTI S (2007). Tuning supramolecular structuring at
the nanoscale level : Nonstoichiometric soluble complexes in dilute mixed solutions of alginate and lactosemodified chitosan (chitlac). BIOMACROMOLECULES, vol. 8(5); p. 1471-1479, ISSN: 1525-7797, doi:
10.1021/BM0610828
• DI FONZO S, MASCIOVECCHIO C, BENCIVENGA F, GESSINI A, FIORETTO D, COMEZ L, MORRESI A, GALLINA
M.E, DE GIACOMO O, CESARO A. (2007). Concentration-temperature dependencies of structural relaxation time in
trehalose-water solutions by Brillouin Inelastic UV Scattering. CHEMTRACTS. ANALYTICAL, PHYSICAL, AND
INORGANIC CHEMISTRY, vol. 111; p. 12577-12583, ISSN: 1048-7840
• CESARO A., DE GIACOMO O, SUSSICH F (2008). Water interplay in trehalose polymorphism. FOOD CHEMISTRY,
vol. 106; p. 1318-1328, ISSN: 0308-8146
• DE GIACOMO O, CESARO A., QUARONI L (2008). Synchrotron based FTIR spectromicroscopy of biopolymer
blends undergoing phase separation. FOOD BIOPHYSICS, vol. 3; p. 77-86, ISSN: 1557-1858
• CESARO A., SUSSICH F (2008). TREHALOSE AMORPHIZATION AND CRYSTALLIZATION. CARBOHYDRATE
• M. BORGOGNA, B. BELLICH, L. ZORZIN, R. LAPASIN, CESARO A. (2009). Food microencapsulation of bioactive
compounds: Rheological and thermal characterisation of non-conventional gelling system. FOOD CHEMISTRY, vol.
In Press, Corrected Proof; p. ---, ISSN: 0308-8146, doi: DOI: 10.1016/j.foodchem.2009.07.043
• A. LERBRET, P. MASON, R. VENABLE, CESARO A., M. SABOUNGI, R. PASTOR, J. BRADY (2009). Molecular
dynamics studies of the conformation of sorbitol. CARBOHYDRATE RESEARCH, vol. 344; p. 2229-2235, ISSN:
0008-6215, doi: DOI: 10.1016/j.carres.2009.08.003
• F. SUSSICH, C. E. SKOPEC, J. W. BRADY, CESARO A. (2009). Water mobility in the dehydration of crystalline
trehalose. FOOD CHEMISTRY, vol. In Press, Corrected Proof; p. ---, ISSN: 0308-8146, doi: DOI:
10.1016/j.foodchem.2009.08.014
• CESARO A. (2006). Bioprotection: All dried up. NATURE MATERIALS, vol. 5(8); p. 593-594, ISSN: 1476-1122
• LERBRET A., MASON P., VENABLE R., CESARO A., SABOUNGI M., PASTOR R., BRADY J. (2009). Molecular
dynamics studies of the conformation of sorbitol. CARBOHYDRATE RESEARCH, vol. 344; p. 2229-2235, ISSN:
0008-6215, doi: 10.1016/j.carres.2009.08.003
• GIANNINI G., CUPPO F., FONTANIVE L., D’, AMELIO N., CESARO A., MAIOCCHI A., UGGERI F. (2011).
Interactions in iodinated contrast media solutions
Part 1. A thermodynamic study. JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY, vol. 103; p. 89-94, ISSN:
1388-6150
• CESARO A., VECCHIO S. (2011). Preface Italian National Conference of Calorimetry and Thermal Analysis.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY, vol. 103; p. 13-14, ISSN: 1388-6150
• BELLICH BARBARA, BORGOGNA MASSIMILIANO, COK MICHELA, CESARO A. (2011). Water evaporation from gel
beads
A calorimetric approach to hydrogel matrix release properties. JOURNAL OF THERMAL ANALYSIS AND
CALORIMETRY; p. 81-88, ISSN: 1388-6150
• FABIANA SUSSICH, CATHERINE E. SKOPEC, JOHN W. BRADY, CESARO A. (2010). Water mobility in the
174
dehydration of crystalline trehalose. FOOD CHEMISTRY; p. 388-393, ISSN: 0308-8146
• BARBARA BELLICH, MASSIMILIANO BORGOGNA, DAMIANO CARNIO, CESARO A. (2009). Thermal behavior of
water in micro-particles based on alginate gel. JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY, vol. 97; p.
871-878, ISSN: 1388-6150
• GALLINA M. E., COMEZ L., PERTICAROLI S., MORRESI A., CESARO A., DE GIACOMO O., DI FONZO S., GESSINI
A., MASCIOVECCHIO C., PALMIERI L., PAOLANTONI M., SASSI P., SCARPONI F., FIORETTO D. (2006). Density
fluctuations of water-glucose mixtures studied by inelastic ultra-violet scattering. PHILOSOPHICAL MAGAZINE, vol.
88; p. 3991-3998, ISSN: 1478-6435
• M. BORGOGNA, B. BELLICH, L. ZORZIN, R. LAPASIN, CESARO A. (2010). Food microencapsulation of bioactive
compounds: Rheological and thermal characterisation of non-conventional gelling system. FOOD CHEMISTRY, vol.
122; p. 416-423, ISSN: 0308-8146, doi: 10.1016/j.foodchem.2009.07.043
• FABIANA SUSSICH, CATHERINE E. SKOPEC, JOHN W. BRADY, CESARO A. (2010). Water mobility in the
dehydration of crystalline trehalose. FOOD CHEMISTRY, vol. 122; p. 388-393, ISSN: 0308-8146
• TAVAGNACCO LETIZIA, MASON PHILIP E., SCHNUPF UDO, PITICI FELICIA, ZHONG LINGHAO, HIMMEL MICHAEL
E., CROWLEY MICHAEL, CESARO A., BRADY JOHN W. (2011). Sugar-binding sites on the surface of the
carbohydrate-binding module of CBH I from Trichoderma reesei. CARBOHYDRATE RESEARCH, vol. 2011; p. "-"-"-",
ISSN: 0008-6215, doi: 10.1016/j.carres.2011.01.019
6. COMELLI Giovanni
• GUNTHER S., HOYER R., MARBACH H., IMBIHL R., ESCH F., AFRICH C., COMELLI G., KISKINOVA M. (2006). K
and Mixed K+O Adlayers on Rh(110). THE JOURNAL OF CHEMICAL PHYSICS, vol. 124; p. 014706-, ISSN: 00219606
• SAVIO L, GERBI A, VATTUONE L, BARALDI A., COMELLI G., ROCCA M (2006). Monitoring super- and subsurface
oxygen on Ag(210) by high energy resolution X-ray photoelectron spectroscopy: Subsurface diffusion and
segregation. JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES
& BIOPHYSICAL, vol. 110; p. 942-, ISSN: 1520-6106
• AFRICH C., COMELLI G. (2006). Scanning Tunneling Microscopy Investigations of Simple Surface Reactions on
Rh(110). JOURNAL OF PHYSICS. CONDENSED MATTER, vol. 18; p. R387-R416, ISSN: 0953-8984, doi:
10.1088/0953-8984/18/22/R01
• BIANCHETTIN L, BARALDI A, DE GIRONCOLI S, LIZZIT S, PETACCIA L, VESSELLI E., COMELLI G., ROSEI R
(2006). Geometric structure of the N/Rh(100) system by core-level photoelctron spectroscopy: Experiment and
theory. PHYSICAL REVIEW. B, CONDENSED MATTER AND MATERIALS PHYSICS, vol. 74; p. 045430-, ISSN: 10980121, doi: 10.1103/PhysRevB.74.045430
• DRI C, AFRICH C., ESCH F, COMELLI G., DUBAY O, KOEHLER L, MITTENDORFER F, KRESSE G, DUDIN P AND
KISKINOVA M (2006). Initial oxidation of the Rh(110) surface: Ordered adsorption and surface oxide structures.
THE JOURNAL OF CHEMICAL PHYSICS, vol. 125; p. 094701-, ISSN: 0021-9606, doi: 10.1063/1.2345058
• BIANCHETTIN L, BARALDI A, VESSELLI E, DE GIRONCOLI S, LIZZIT S, PETACCIA L, COMELLI G., ROSEI R
(2007). Experimental and Theoretical Surface Core Level Shift Study of the S-Rh(100) Local Environment.
JOURNAL OF PHYSICAL CHEMISTRY. C, NANOMATERIALS AND INTERFACES, vol. 111; p. 4003-, ISSN: 1932-7447
• BARALDI A, BIANCHETTIN L, VESSELLI E, DE GIRONCOLI S, LIZZIT S, PETACCIA L, ZAMPIERI G, COMELLI G.,
ROSEI R (2007). Highly under-Coordinated Atoms at Rh Surfaces: Interplay of Strain and Coordination Effects on
Core Level Shift. NEW JOURNAL OF PHYSICS, vol. 9; p. 143-, ISSN: 1367-2630
• BARALDI A, VESSELLI E., BIANCHETTIN L, COMELLI G., LIZZIT S, PETACCIA L, DE GIRCONCOLI S, LOCATELLI
A, MENTES O.T, ABALLE L, WEISSENRIEDER J, ANDERSEN J.N (2007). The (1x1)-> hexagonal phase transition on
Pt(100) studied by high resolution core level photoemission. THE JOURNAL OF CHEMICAL PHYSICS, vol. 127; p.
164702-, ISSN: 0021-9606, doi: 10.1063/1.2794344
• DING X, DE ROGATIS L, VESSELLI E, BARALDI A, COMELLI G., ROSEI R, SAVIO L, VATTUONE L, ROCCA M,
FORNASIERO P, ANCILOTTO F, BALDERESCHI A, PERESSI M. (2007). Interaction of carbon dioxide with Ni(110): a
• L. BIANCHETTIN, BARALDI A., S. DE GIRONCOLI, E. VESSELLI, S. LIZZIT, L. PETACCIA, COMELLI G., R. ROSEI
(2008). Core level shifts of under-coordinated Pt atoms. THE JOURNAL OF CHEMICAL PHYSICS, vol. 128; p.
114706-, ISSN: 0021-9606
• E. VESSELLI, L. BIANCHETTIN, BARALDI A., A. SALA, COMELLI G., L. PETACCIA, S. LIZZIT, S. DE GIRONCOLI
(2008). The Ni3Al(111) surface structure: experiment and theory. JOURNAL OF PHYSICS. CONDENSED MATTER,
vol. 20; p. 195223-, ISSN: 0953-8984
• VESSELLI E, DE ROGATIS L, DING X, BARALDI A, SAVIO L, VATTUONE L, ROCCA M, FORNASIERO P, PERESSI
M., BALDERESCHI A, ROSEI R, COMELLI G. (2008). Carbon Dioxide Hydrogenation on Ni(110). JOURNAL OF THE
AMERICAN CHEMICAL SOCIETY, vol. 130(34); p. 11417-11422, ISSN: 0002-7863, doi: 10.1021/JA802554G
• VESSELLI E, CAMPANIELLO M, BARALDI A, BIANCHETTIN L, AFRICH C, ESCH F, LIZZIT S, COMELLI G. (2008). A
Surface Core Level Shift Study of Hydrogen-Induced Ordered Structures on Rh(110). JOURNAL OF PHYSICAL
• AFRICH C, KÖHLER L, ESCH F, CORSO M, DRI C, BUCKO T, KRESSE G, COMELLI G. (2009). Effects of the Lattice
Expansion on the Reactivity of a 1D Oxide. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 131; p. 3253,
ISSN: 0002-7863
• BIANCHETTIN L, BARALDI A, DE GIRONCOLI S, VESSELLI E, LIZZIT S, COMELLI G., ROSEI R (2009). Surface
Core Level Shift: High Sensitive Probe to Oxygen-Induced Reconstruction of Rh(100). JOURNAL OF PHYSICAL
• DE ROGATIS L, VESSELLI E, BARALDI A, CASULA M.F, MONTINI T, COMELLI G., GRAZIANI M, FORNASIERO P.
• VESSELLI E, RIZZI M, DE ROGATIS L, DING X, BARALDI A, COMELLI G., SAVIO L, VATTUONE L, ROCCA M, P.
FORNASIERO, BALDERESCHI A, PERESSI M (2010). Hydrogen-assisted transformation of CO2 on nickel: the role of
175
formate and carbon monoxide. THE JOURNAL OF PHYSICAL CHEMISTRY LETTERS, vol. 1; p. 402-406, ISSN: 19487185, doi: 10.1021/jz900221c.
• DRI C., PERONIO A., VESSELLI E., AFRICH C., RIZZI M., BALDERESCHI A., PERESSI M., COMELLI G. (2010).
Imaging and characterization of activated CO2 species on Ni(110). PHYSICAL REVIEW. B, CONDENSED MATTER
AND MATERIALS PHYSICS, vol. 82; p. 165403--(1-6), ISSN: 1098-0121, doi: 10.1103/PhysRevB.82.165403
• COLUSSI S., TROVARELLI A., VESSELLI E., BARALDI A., COMELLI G., GROPPI G., LLORCA J. (2010). Structure
and morphology of Pd/Al2O3 and Pd/CeO2/Al2O3 combustion catalysts in Pd–PdO transformation hysteresis.
APPLIED CATALYSIS A: GENERAL, vol. 390; p. 1-10, ISSN: 0926-860X, doi: 10.1016/j.apcata.2010.09.033
• BARALDI A., BIANCHETTIN L., DE GIRONCOLI S., VESSELLI E., LIZZIT S., PETACCIA L., COMELLI G., ROSEI R.
(2011). Enhanced Chemical Reactivity of Under-Coordinated Atoms at Pt-Rh
Bimetallic Surfaces: A Spectroscopic Characterization. JOURNAL OF PHYSICAL CHEMISTRY. C, NANOMATERIALS
AND INTERFACES, vol. 115; p. 3378-3384, ISSN: 1932-7447, doi: 10.1021/jp110329w
7. DA ROS Tatiana
• CECCHET F, RAPINO S, MARGOTTI M, DA ROS T., PRATO M, PAOLUCCI F, RUDOLF P (2006). Structural and
electrochemical characterization of fullerene-based surfaces of C60 mono- or bis-adducts grafted onto selfassembled monolayers. CARBON, vol. 44; p. 3014-3021, ISSN: 0008-6223
• CUSAN C., ALTINIER G., SOSA S., SIBILLA F., BUCAR F., TUBARO A., PRATO M., SPALLUTO G., DA ROS T.
(2006). Anti-inflammatory and anti-oxidant activity of a new class of phenyl-pyrazolone derivatives. CURRENT
DRUG DISCOVERY TECHNOLOGIES, vol. 3; p. 67-73, ISSN: 1570-1638
• PASTORIN G, DA ROS T., BOLCATO C, MONTOPOLI C, MORO S, CACCIARI B, BARALDI P.G, VARANI K, BOREA
P.A, SPALLUTO G (2006). Synthesis and Biological Studies of a New Series of 5Heteroarylcarbamoylaminopyrazolo[4,3-e]1,2,4-triazolo[1,5-c]pyrimidines as Human A3 Adenosine Receptor
Antagonists. Influence of the Heteroaryl Substituent on Binding Affinity and Molecular Modeling Investigations.
JOURNAL OF MEDICINAL CHEMISTRY, vol. 49; p. 1720-1729, ISSN: 0022-2623
• PASTORIN G, MARCHESAN S, HOEBEKE J, DA ROS T., EHRET-SABATIER L, BRIAND J.-P, PRATO M, BIANCO A
(2006). Design and Activity of Cationic Fullerene Derivatives as Inhibitors of Acetylcholinesterase. ORGANIC &
BIOMOLECULAR CHEMISTRY, vol. 4; p. 2556-2562, ISSN: 1477-0520
• ROSS M.F, DA ROS T., BLAIKIE F.H, PRIME T.A, PORTEOUS C.M, SEVERINA I.I, SKULACHEV V.P, KJAERGAARD
H.G, SMITH R.A.J, MURPHY M.P (2006). Accumulation of lipophilic dications by mitochondria and cells.
BIOCHEMICAL JOURNAL, vol. 400; p. 199-208, ISSN: 0264-6021
• KLUMPP C, LACERDA L, CHALOIN O, DA ROS T., KOSTARELOS K, PRATO M, BIANCO A (2007).
Multifunctionalised cationic fullerene adducts for gene transfer: design, synthesis and DNA complexation.
CHEMICAL COMMUNICATIONS; p. 3762-3764, ISSN: 1359-7345
• CATALDO F, DA ROS T. (a cura di) (2008). Medicinal Chemistry and Pharmacological Potential of Fullerenes and
Carbon Nanotubes. Springer, vol. 1, ISBN: 9781402068447
• HERRERO MA, TOMA FM, AL-JAMAL KT, KOSTARELOS K, BIANCO A, DA ROS T., BANO F, CASALIS L, SCOLES G,
PRATO M (2009). Synthesis and Characterization of a Carbon Nanotube−Dendron Series for Efficient siRNA
Delivery. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 131; p. 9843-9848, ISSN: 0002-7863, doi:
10.1021/ja903316z
• C. MÉNARD-MOYON, E. VENTURELLI, C. FABBRO, C. SAMORÌ, DA ROS T., K. KOSTARELOS, M. PRATO, A.
BIANCO (2010). The alluring potential of functionalized carbon nanotubes in drug discovery. EXPERT OPINION ON
DRUG DISCOVERY, vol. 5; p. 691-707, ISSN: 1746-0441
• GURYANOV I., TOMA F.M., MONTELLANO LÓPEZ A., CARRARO M., DA ROS T., ANGELINI G., D’, AURIZIO E.,
FONTANA A., MAGGINI M., PRATO M., BONCHIO M. (2009). Microwave-assisted functionalization of carbon
nanostructures in ionic liquids. CHEMISTRY-A EUROPEAN JOURNAL; p. 12837-12845, ISSN: 0947-6539
• C. SAMORÌ, H. ALI-BOUCETTA, R. SAINZ, C. GUO, F. M. TOMA, C. FABBRO, DA ROS T., M. PRATO, K.
KOSTARELOS, A. BIANCO (2010). Enhanced anticancer activity of multi-walled carbon nanotube-methotrexate
conjugates using cleavable linkersw. CHEMICAL COMMUNICATIONS, vol. 46; p. 1494-1496, ISSN: 1359-7345
• GURYANOV I., MONTELLANO LÓPEZ A., CARRARO M., DA ROS T., SCORRANO G., MAGGINI M., PRATO M.,
BONCHIO M. (2009). Metal-free, retro-cycloaddition of fulleropyrrolidines in ionic liquids under microwave
irradiation. CHEMICAL COMMUNICATIONS; p. 3940-3942, ISSN: 1359-7345
• TOMA F.M., SARTOREL A., IURLO M., CARRARO M., PARISSE P., MACCATO C., RAPINO S., RODRIGUEZ
GONZALEZ B., AMENITSCH H., DA ROS T., CASALIS L., GOLDON A., MARCACCIO M., SCORRANO G., SCOLES G.,
PAOLUCCI F., PRATO M., BONCHIO M. (2010). Efficient water oxidation at carbon nanotube–polyoxometalate
electrocatalytic interfaces. NATURE CHEMISTRY; p. 826-831, ISSN: 1755-4330
8. DE VITA Alessandro
• G.MORAS, G.CSANYI, M.C.PAYNE, A. DE VITA (2006). A novel molecular dynamics approach to large
semiconductor systems. PHYSICA. B, CONDENSED MATTER, vol. 376-377; p. 936-939, ISSN: 0921-4526
• M.RUBEN, D.PAYER, A.LANDA, A.COMISSO, C.GATTINONI, N.LIN, J.-P.COLLIN, J.-P. SAUVAGE, A. DE VITA ,
K.KERN (2006). 2D Supramolecular Assemblies of Benzene 1,3,5-tri-yl Tribenzoic Acid: Temperature-Induced
Phase Transformations and Hierarchical Organization with Macrocyclic Molecules. JOURNAL OF THE AMERICAN
CHEMICAL SOCIETY, vol. 128; p. 15644, ISSN: 0002-7863
• D. PAYER, A. COMISSO, A. DMITRIEV, T. STRUNSKUS, N. LIN, C. WLL, A. DE VITA , J. V. BARTH, K. KERN
(2007). Ionic Hydrogen Bonds Controlling Two-Dimensional Supramolecular Systems at a Metal Surface.
CHEMISTRY-A EUROPEAN JOURNAL, vol. 13; p. 3900, ISSN: 0947-6539
• M.LINGENFELDER, G.TOMBA, G.COSTANTINI, L.COLOMBI CIACCHI, A. DE VITA , KLAUS KERN (2007). Tracking
the Chiral Recognition of Adsorbed Dipeptides at the Single-Molecule Level. ANGEWANDTE CHEMIE.
INTERNATIONAL EDITION, vol. 46; p. 1, ISSN: 1433-7851
• F.KLAPPENBERGER, M.E. CAAS-VENTURA, S.CLAIR, S.PONS, U.SCHLICKUM, Z.-R. QU, H.BRUNE, K. KERN, T.
STRUNSKUS, C. WLL, A.COMISSO, A. DE VITA , M. RUBEN, J.V. BARTH (2007). Conformational adaptation in
supramolecular assembly on surfaces. CHEMPHYSCHEM, vol. 8; p. 1782, ISSN: 1439-4235
• S.PISCANEC, F.ZULIANI, L.C.CIACCHI, G.LEVITA, O.SBAIZERO, A. DE VITA (2007). Density Functional-based
176
Atomistic Modelling of Ceramic Materials and Interfaces. INTERNATIONAL JOURNAL OF MATERIALS & PRODUCT
TECHNOLOGY, vol. ?; p. ?, ISSN: 0268-1900
• J.R. KERMODE, T. ALBARET, D. SHERMAN, N. BERNSTEIN, P. GUMBSCH, M.C. PAYNE, G. CSANYI, A. DE VITA
(2008). Low speed fracture instabilities in a brittle crystal. NATURE, vol. 455; p. 1224, ISSN: 0028-0836
• F.ZULIANI, R.CHOUDHURY, O.SBAIZERO, A. DE VITA (2008). Dipole formation due to ion-exchange processes at
the metallic-ceramic Al/MgAl2O4 (001) interface. ACTA MATERIALIA, vol. 55; p. 5813, ISSN: 1359-6454
• G.TOMBA, M.LINGENFELDER, G.COSTANTINI, K.KERN, F.KLAPPENBERGER, J.V. BARTH, L. COLOMBI CIACCHI, A.
DE VITA (2007). Structure and energetics of diphenylalanine self-assembling on Cu(110). JOURNAL OF PHYSICAL
CHEMISTRY. A, MOLECULES, SPECTROSCOPY, KINETICS, ENVIRONMENT, & GENERAL THEORY, vol. 111; p. 12740,
ISSN: 1089-5639
• G.MORAS, L. COLOMBI CIACCHI, C.CSANYI, A. DE VITA (2007). Modeling (100) hydrogen-induced platelets in
silicon: a
multiscale molecular dynamics approach. PHYSICA. B, CONDENSED MATTER, vol. 401; p. 16, ISSN: 0921-4526
• G.TOMBA, L.COLOMBI CIACCHI, A. DE VITA (2009). Atomic-Level Studies of Molecular Self-Assembly on Metallic
Surfaces. ADVANCED MATERIALS, vol. 21; p. 1-12, ISSN: 0935-9648
• F.ZULIANI, L.C.CIACCHI, G.LEVITA, O.SBAIZERO, A. DE VITA (2009). DFT modelling of ceramic materials and
interfaces. INTERNATIONAL JOURNAL OF MATERIALS & PRODUCT TECHNOLOGY, vol. 35; p. 271, ISSN: 0268-1900
• G. LEVITA, L.PETACCIA, A.COMISSO, S.LIZZIT, R.LARCIPRETE, A.GOLDONI, A. DE VITA (2008). A spectroscopic
and ab initio study of the formation of graphite and carbon nanotubes from thermal decomposition of silicon
carbide. NANO LETTERS, vol. 8; p. 4335, ISSN: 1530-6984
• VITALI L, LEVITA G, OHMANN R, COMISSO A, A. DE VITA , KERN K. (2010). Portrait of the potential barrier at
metal-organic nanocontacts. NATURE MATERIALS, vol. 9/2010; p. 320-323, ISSN: 1476-1122
• MORAS, G, CIACCHI, LC, ELSASSER, C, GUMBSCH P, A. DE VITA , A. (2010). Atomically Smooth StressCorrosion Cleavage of a Hydrogen-Implanted Crystal. PHYSICAL REVIEW LETTERS, vol. 105/2010; p. 075502--,
ISSN: 0031-9007
9. DI LENARDA Roberto
• CADENARO M., BRESCHI L, ANTONIOLLI F, MAZZONI A, DI LENARDA R. (2006). Influence of whitening on the
degree of conversion of dental adhesives on dentin. EUROPEAN JOURNAL OF ORAL SCIENCES, vol. 114(3); p. 257262, ISSN: 0909-8836
• VENTURI M, DI LENARDA R., BRESCHI L (2006). AN EX VIVO COMPARISON OF THREE DIFFERENT GUTTAPERCHA CONES WHEN COMPACTED AT DIFFERENT TEMPERATURES: RHEOLOGICAL CONSIDERATIONS IN
RELATION TO THE FILLING OF LATERAL CANALS. INTERNATIONAL ENDODONTIC JOURNAL, vol. 39(8); p. 648656, ISSN: 0143-2885, doi: 10.1111/J.1365-2591.2006.01133.X
• PASQUANTONIO G, TAY FR, MAZZONI A, SUPPA P, RUGGERI A JR, FALCONI M, DI LENARDA R., BRESCHI L.
(2007). Electric device improves bonds of simplified etch-and-rinse adhesives. DENTAL MATERIALS, vol. 23; p.
513-518, ISSN: 0109-5641
• CADENARO M., BIASOTTO M, CONTARDO L, CHIESA R, DI LENARDA R., DORIGO E (2006). Surface roughness of
three resin restorative materials after finishing and polishing. MINERVA STOMATOLOGICA, vol. 55(4); p. 179-187,
ISSN: 0026-4970
• VISINTINI E., RIZZARDI C, CHIANDUSSI S, BIASOTTO M, MELATO M, DI LENARDA R. (2006). Xantoma
verruciforme della mucosa orale. Descrizione di un caso clinico. MINERVA STOMATOLOGICA, vol. 55; p. 639-645,
ISSN: 0026-4970
• BIASOTTO M, CHIANDUSSI S, DORE F, RINALDI A, RIZZARDI C, CAVALLI F, DI LENARDA R. (2006). Clinical
aspects and management of bisphosphonates-associated osteonecrosis of the jaws. ACTA ODONTOLOGICA
SCANDINAVICA, vol. 64; p. 348-354, ISSN: 0001-6357
• BRESCHI L, MAZZONI A, PASHLEY DH, PASQUANTONIO G, RUGGERI A, SUPPA P, MAZZOTTI G, DI LENARDA R.,
TAY FR (2006). Electric-current-assisted Application of Self-etch Adhesives to Dentin. JOURNAL OF DENTAL
• RUGGERI A JR, PRATI C, MAZZONI A, NUCCI C, DI LENARDA R., MAZZOTTI G, BRESCHI, L (2007). Effects of
citric acid and EDTA conditioning on exposed root dentin: An
immunohistochemical analysis of collagen and proteoglycans. ARCHIVES OF ORAL BIOLOGY, vol. 52; p. 1-8, ISSN:
0003-9969
• CHIANDUSSI S, BIASOTTO M, DORE F, CAVALLI F, COVA MA, DI LENARDA R. (2006). Clinical and diagnostic
imaging of bisphosphonate-associated osteonecrosis of
the jaws. DENTOMAXILLOFACIAL RADIOLOGY, vol. 35; p. 236-243, ISSN: 0250-832X
• L. MOIMAS, M. BIASOTTO, DI LENARDA R., A. OLIVO, SCHMID C. (2006). Rabbit pilot study on the resorbability
of three-dimensional bioactive glass fibre scaffolds. ACTA BIOMATERIALIA, vol. 2; p. 191-199, ISSN: 1742-7061
• BRESCHI L, MAZZONI A, RUGGERI A, CADENARO M, DI LENARDA R., DE STEFANO DORIGO E (2008). Dental
• MAZZONI A, MANNELLO F, TAY FR, TONTI GAM, PAPA S, MAZZOTTI G, DI LENARDA R., PASHLEY DH, BRESCHI
L. (2007). Zymographic analysis and characterization of MMP-2 and -9 forms in human sound dentin. JOURNAL OF
• ORSINI G, RUGGERI A JR, MAZZONI A, PAPA V, MAZZOTTI G, DI LENARDA R., BRESCHI L (2007).
Immunohistochemical identification of decorin and biglycan in human dentin: a correlative field emission scanning
electron microscopy/transmission electron microscopy study. CALCIFIED TISSUE INTERNATIONAL, vol. 81; p. 3945, ISSN: 0171-967X
• ORSINI G, RUGGERI A JR, MAZZONI A, PAPA V, PICCIRILLI M, FALCONI M, DI LENARDA R., BRESCHI L. (2007).
Immunohistochemical identification of type I and type III collagen and chondroitin sulphate in human predentine: a
correlative FEI-SEM/TEM study. INTERNATIONAL ENDODONTIC JOURNAL, vol. 40; p. 669-678, ISSN: 0143-2885
• BRESCHI L, CADENARO M., ANTONIOLLI F, VISINTINI E, TOLEDANO M, DI LENARDA R. (2007). Extent of
177
275-280, ISSN: 0894-8275
• CONTARDO L, DE LUCA M, BEVILACQUA L., BRESCHI L, DI LENARDA R. (2007). Influence of calcium hydroxide
debris on the quality of endodontic apical seal. MINERVA STOMATOLOGICA, vol. 56(10); p. 509-517, ISSN: 00264970
• BEVILACQUA L., SOSSI A, CADENARO M, DI LENARDA R. (2007). Comparative evaluation of the microhardness
of 4 dental sealants. EUROPEAN JOURNAL OF PAEDIATRIC DENTISTRY, vol. 8 (4); p. 179-182, ISSN: 1591-996X
• BEVILACQUA L, CADENARO M., SOSSI A, BIASOTTO M, DI LENARDA R. (2007). Influence of air abrasion and
etching on enamel and adaptation of a dental sealant. EUROPEAN JOURNAL OF PAEDIATRIC DENTISTRY, vol. 8(1);
p. 25-30, ISSN: 1591-996X
• VISINTINI E, MAZZONI A, VITA F, PASQUANTONIO G, CADENARO M., DI LENARDA R., BRESCHI L (2008).
Effects of thermocycling and use of ElectroBond on microtensile strength and nanoleakage using commercial onestep self-etch adhesives. EUROPEAN JOURNAL OF ORAL SCIENCES, vol. 116(6); p. 564-570, ISSN: 0909-8836,
doi: DOI: 10.1111/J.1600-0722.2008.00576.X
• CADENARO M, BRESCHI L, RUEGGEBERG FA, AGEE K, DI LENARDA R., CARRILHO M, TAY FR, PASHLEY DH
• MAZZONI A, PASHLEY DH, TAY FR, GOBBI P, ORSINI G, RUGGERI A JR, CARRILHO M, TJÄDERHANE L, DI
LENARDA R., BRESCHI L (2009). Immunohistochemical identification of MMP-2 and MMP-9 in human dentin:
Correlative FEI-SEM/TEM analysis. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A, vol. 88; p. 697-703,
ISSN: 1549-3296
• CADENARO M, BIASOTTO M., SCUOR N, BRESCHI L, DAVIDSON CL, DI LENARDA R. (2008). Assessment of
0109-5641, doi: 10.1016/J.DENTAL.2007.06.031
• CADENARO M., BRESCHI L, ANTONIOLLI F, NAVARRA CO, MAZZONI A, TAY FR, DI LENARDA R., PASHLEY DH
vol. 24; p. 1194-1200, ISSN: 0109-5641
• CADENARO M., BRESCHI L, NUCCI C, ANTONIOLLI F, VISINTINI E, PRATI C, MATIS BA, DI LENARDA R. (2008).
• MAZZONI A, PASHLEY DH, RUGGERI A JR, VITA F, FALCONI M, DI LENARDA R., BRESCHI L. (2008). Adhesion to
chondroitinase ABC treated dentin. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART B, APPLIED
BIOMATERIALS, vol. 86B; p. 228-236, ISSN: 1552-4981
• ORSINI G, RUGGERI A JR, MAZZONI A, NATO A, FALCONI M, DI LENARDA R., NANCI A, BRESCHI L (2008).
Immunohistochemical Localization of DMP1 in Human Dentin. EUROPEAN JOURNAL OF HISTOCHEMISTRY, vol. 52;
p. 215-220, ISSN: 1121-760X
• STACCHI C, ORSINI G, DI IORIO D, BRESCHI L., DI LENARDA R. (2008). Clinical, histologic and
histomorphometric analyses of regenerated bone in maxillary sinus augmentation using fresh frozen human bone
allografts. JOURNAL OF PERIODONTOLOGY, vol. 79; p. 1789-1796, ISSN: 0022-3492
• BRESCHI L, CADENARO M., ANTONIOLLI F, SAURO S, BIASOTTO M, PRATI C, TAY FR, DI LENARDA R. (2007).
• SUPPA P, BRESCHI L, BIASOTTO M, MAZZONI A, VISINTINI E., RUGGERI A, DI LENARDA R. (2007). Efficacia di
differenti sistemi di cementazione intracanalare mediante test di microtensile. GIORNALE ITALIANO DI
CONSERVATIVA, vol. 5; p. 168-172, ISSN: 1724-2908
• CONTARDO L., CESCHI M, CASTALDO A, DENOTTI G, DI LENARDA R. (2008). Differences in skeletal Class II
diagnosis using various cephalometric analyses. JOURNAL OF CLINICAL ORTHODONTICS, vol. 42; p. 389-392,
ISSN: 0022-3875
• STACCHI C, COSTANTINIDES F, BIASOTTO M., DI LENARDA R. (2008). Relocation of a malpositioned maxillary
implant with piezoelectric osteotomies: a case report. THE INTERNATIONAL JOURNAL OF PERIODONTICS &
RESTORATIVE DENTISTRY, vol. 28(5); p. 489-495, ISSN: 0198-7569
• DE IUDICIBUS S, CASTRONOVO G, GIGANTE A, STOCCO G, DECORTI G, DI LENARDA R., BARTOLI F (2008).
Role of MDR1 gene polymorphisms in gingival overgrowth induced by cyclosporine in transplant patients. JOURNAL
OF PERIODONTAL RESEARCH, vol. 43; p. 665-672, ISSN: 0022-3484
• DORE F, FILIPPI L, BIASOTTO M., CHIANDUSSI S, CAVALLI F, DI LENARDA R. (2009). Bone Scintigraphy and
SPECT/CT of Bisphosphonate-Induced Osteonecrosis of the Jaw. THE JOURNAL OF NUCLEAR MEDICINE, vol. 50(1);
p. 30-35, ISSN: 0161-5505
• CADENARO M., BRESCHI L, RUEGGEBERG FA, SUCHKO M, GRODIN E, AGEE K, DI LENARDA R., TAY FR,
PASHLEY DH (2009). Effects of residual ethanol on the rate and degree of conversion of five experimental resins.
DENTAL MATERIALS, vol. 25(5); p. 621-628, ISSN: 0109-5641, doi: 10.1016/J.DENTAL.2008.11.005
• COSTANTINIDES F, BIASOTTO M., GREGORI D, MAGLIONE M, DI LENARDA R. (2009). "Abscess" as a
perioperative risk factor for paresthesia after third molar extraction under general anesthesia. ORAL SURGERY
ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS, vol. 107(2); p. 8-13, ISSN: 1079-2104
• TIRELLI G, BIASOTTO M, CHIANDUSSI S, DORE F, DE NARDI E, DI LENARDA R. (2009). Bisphosphonateassociated osteonecrosis of the jaws: the limits of a conservative approach. HEAD & NECK, vol. 31(9); p. 12491254, ISSN: 1043-3074, doi: 10.1002/HED.21019
• MAZZONI A, VISINTINI E, VITA F, PASQUANTONIO G, SABOIA VP, RUGGERI A JR, DI LENARDA R., DE STEFANO
DORIGO E., BRESCHI L (2009). ElectroBond improves immediate dentin microtensile bond strength of two etchand-rinse adhesives. JOURNAL OF ADHESIVE DENTISTRY, vol. 11(1); p. 27-33, ISSN: 1461-5185, doi:
DOI:10.3290/J.JAD.A14729
• CADENARO M, PASHLEY DH, MARCHESI G, CARRILHO M, ANTONIOLLI F, MAZZONI A, TAY FR, DI LENARDA R.,
10.1016/J.DENTAL.2009.05.008
178
• NAVARRA C.O, CADENARO M, ARMSTRONG S.R, JESSOP J, ANTONIOLLI F, SERGO V., DI LENARDA R., BRESCHI
10.1016/J.DENTAL.2009.05.009
• BRESCHI L, CAMMELLI F, VISINTINI E, MAZZONI A, VITA F, CARRILHO M, CADENARO M., FOULGER S, MAZZOTI
G, TAY FR, DI LENARDA R., PASHLEY D (2009). Influence of chlorhexidine concentration on the durability of etchand-rinse dentin bonds: a 12-month in vitro study. JOURNAL OF ADHESIVE DENTISTRY, vol. 11; p. 191-198,
ISSN: 1461-5185
• MAZZONI A, MARCHESI G, CADENARO M, MAZZOTTI G, DI LENARDA R., FERRARI M, BRESCHI L. (2009). Pushout stress for fibre posts luted using different adhesive strategies. EUROPEAN JOURNAL OF ORAL SCIENCES, vol.
117; p. 447-453, ISSN: 0909-8836
• CADENARO M., DELISE C, ANTONIOLLI F, NAVARRA CO, DI LENARDA R., BRESCHI L (2009). Enamel and dentin
ISSN: 1461-5185
• CHIANDUSSI S., LUZZATI R., TIRELLI G., DI LENARDA R., BIASOTTO M. (2009). Cancrum oris in developed
Countries. AGING CLINICAL AND EXPERIMENTAL RESEARCH, vol. 21 (6)/2009; p. 475-477, ISSN: 1594-0667
• BORELLI V, MARCHIOLI A, DI TARANTO R, ROMANO M, CHIANDUSSI S, DI LENARDA R., BIASOTTO M,
ZABUCCHI G. (2010). Neuropeptides in saliva of subjects with burning mouth syndrome: a pilot study. ORAL
DISEASES, vol. 16(4); p. 365-374, ISSN: 1354-523X, doi: 10.1111/j.1601-0825.2009.01648.x
• TIRELLI G, RIZZO R, BIASOTTO M, DI LENARDA R., ARGENTI B, GATTO A, BULLO F. (2010). Obturator
prostheses following palatal resection: clinical cases. ACTA OTORHINOLARYNGOLOGICA ITALICA, vol. 30/1; p. 3339, ISSN: 0392-100X
• TASCHNER M, NATO F, MAZZONI A, FRANKENBERGER R, KRÄMER N, DI LENARDA R., PETSCHELT A, BRESCHI L.
(2010). Role of preliminary etching for one-step self-etch adhesives. EUROPEAN JOURNAL OF ORAL SCIENCES, vol.
118; p. 517-524, ISSN: 0909-8836, doi: 10.1111/j.1600-0722.2010.00769.x.
• MARCHESI G, BRESCHI L, ANTONIOLLI F, DI LENARDA R., FERRACANE J, CADENARO M. (2010). Contraction
p. 947-953, ISSN: 0109-5641
• BIASOTTO M, CHIANDUSSI S, ZACCHIGNA S, MOIMAS S, DORE F, POZZATO G, CAVALLI F, ZANCONATI F,
CONTARDO L, GIACCA M, DI LENARDA R. (2010). A novel animal model to study non-spontaneous
bisphosphonates osteonecrosis of jaw. JOURNAL OF ORAL PATHOLOGY & MEDICINE, vol. 39; p. 390-396, ISSN:
0904-2512
10. FASSINA Ambrogio
11. FERMEGLIA Maurizio
• TOTH R, FERRONE M., MIERTUS S, CHIELLINI E, FERMEGLIA M., PRICL S (2006). Structure and energetics of
biocompatible polymer nanocomposite sistems: a molecular dynamics study. BIOMACROMOLECULES, vol. 7; p.
1714-1719, ISSN: 1525-7797
• FERMEGLIA M., COSOLI P, FERRONE M., PICCAROLO S, MENSITIERI G, PRICL S (2006). PET/PEN Blends of
Industrial Interest as Barrier Materials. Part I. Many-Scale Molecular Modeling of PET/PEN Blends. POLYMER, vol.
47; p. 5979-5989, ISSN: 0032-3861
• FERMEGLIA M., PRICL S (2006). Self-Assembly of Nanoparticle Mixtures in Lamellar Diblock Copolymers:
multiscale molecular modeling. In: AIZ Workshop 2006, Innovative Applications of Layered Materials: from
Catalysis to Nanotechnology. Alessandria, 1.9.2006, ALESSANDRIA: Leonardo Marchese, vol. 1, p. 1-10
• SCOCCHI G, POSOCCO P, FERMEGLIA M., PRICL S. (2007). Polymer-Clay Nanocomposites: A Multiscale
Molecular Modeling Approach. JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS,
SURFACES, INTERFACES & BIOPHYSICAL, vol. 111; p. 2143-2151, ISSN: 1520-6106
• POSOCCO P, FERRONE M, FERMEGLIA M., PRICL S. (2007). Binding at the core. Computational study of
structural and ligand binding properties of naphthiridine based dendrimers. MACROMOLECULES, vol. 40; p. 22572266, ISSN: 0024-9297
• FERMEGLIA M., PRICL S (2007). Multiscale modeling for polymer systems of industrial interest. PROGRESS IN
ORGANIC COATINGS, vol. 58; p. 18-199, ISSN: 0300-9440
• SCOCCHI G, POSOCCO P, PRICL S, FERMEGLIA M. (2007). To the nanoscale and beyond! Multiscale molecular
modelling of polymer-clay nanocomposites. FLUID PHASE EQUILIBRIA, vol. 261; p. 366-374, ISSN: 0378-3812
• CARTA A, LORIGA M, PAGLIETTI G, FERRONE M, FERMEGLIA M., PRICL S., SANNA T, IBBA C, LA COLLA P,
LODDO R (2007). Design, synthesis and preliminary in vitro and in silico antiviral activity of [4,7]phenantrolines
and 1-oxo-1,4-dihydro-[4,7]phenantrolines against a single-stranded positive sense RNA genome viruses.
BIOORGANIC & MEDICINAL CHEMISTRY, vol. 15; p. 1914-1927, ISSN: 0968-0896
• COSOLI P, FERRONE M, PRICL S, FERMEGLIA M. (2007). Grand canonical Monte Carlo simulations for VOCs
adsorption in non-polar zeolites. INTERNATIONAL JOURNAL OF ENVIRONMENTAL TECHNOLOGY AND
MANAGEMENT, vol. 7; p. 228-243, ISSN: 1466-2132
• PRICL S, FERRONE M, FERMEGLIA M., AMATO F, COSENTINO C, CHENG M.M.C, WALCZAK R, FERRARI M (2006).
Multiscale modeling of protein transport in silicon membrane nanochannels. Part 1. Derivation of molecular
parameters from computer simulations. BIOMEDICAL MICRODEVICES, vol. 8(4); p. 277-290, ISSN: 1387-2176,
doi: 10.1007/S10544-006-0031-2
• PRICL S, FERRONE M, FERMEGLIA M., AMATO F, COSENTINO C, CHENG M.M.C, WALCZAK R, FERRARI M (2006).
Multiscale modeling of protein transport in silicon membrane nanochannels. Part 2. From molecular parameters to
a predictive continuum diffusion model. BIOMEDICAL MICRODEVICES, vol. 8; p. 291-298, ISSN: 1387-2176
• FERMEGLIA M., BRAIUCA P, GARDOSSI L., PRICL S, HALLING PJ (2006). In silico prediction of medium effects on
esterification equilibrium using the COSMO-RS method. BIOTECHNOLOGY PROGRESS, vol. 22; p. 1146-1152,
ISSN: 8756-7938
• BANFI E., SCIALINO G., ZAMPIERI D., MAMOLO M.G., VIO L., FERRONE M., FERMEGLIA M., PANENI M.S., PRICL
179
S. (2006). Antifungal and antimycobacterial activity of new imidazole and triazole derivatives. A combined
experimental and computational approach. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, vol. 58; p. 76-84,
ISSN: 0305-7453
• DONOLO G, DE SIMON G, FERMEGLIA M. (2006). Steady state dimulation of energy production from biomass by
molten carbonate fuel cells. JOURNAL OF POWER SOURCES, vol. 158; p. 1282-1289, ISSN: 0378-7753
• CARTA A, LORIGA G, PIRASA S, PAGLIETTI G, LA COLLA P, COLLU G, PIANO M.A, LODDO R, FERRONE M,
FERMEGLIA M., PRICL S. (2006). Synthesis and in vitro evaluation of the anti-viral activity of N-[4-(1H(2H)benzotriazol-1(2)-yl)phenyl]alkylcarboxamides. MEDICINAL CHEMISTRY, vol. 2; p. 577-589, ISSN: 1573-4064
• COSOLI P, SCOCCHI G, PRICL S., FERMEGLIA M. (2008). Many-scale molecular simulation for ABS–MMT
nanocomposites: Upgrading of industrial scraps. MICROPOROUS AND MESOPOROUS MATERIALS, vol. 107; p. 169179, ISSN: 1387-1811
• FERRONE M., PERRONE F, TAMBORINI E, PANENI M.S, FERMEGLIA M., SUARDI S, PASTORE E, DELIA D,
PIEROTTI M.A, PRICL S, PILOTTI S (2006). Functional analysis and molecular modelling show a preserved wild type
activity of p53 C238Y. MOLECULAR CANCER THERAPEUTICS, vol. 5; p. 1467-1473, ISSN: 1535-7163
• TAMBORINI E, PRICL S., NEGRI T, LAGONIGRO M.S, MISELLI F, GRECO A, GRONCHI A, CASALI P, FERRONE M,
FERMEGLIA M., PIEROTTI M.A, PILOTTI S (2006). Functional analyses and Molecular modeling of two c-Kit
mutations responsible for Imatinib secondary resistance in GIST patients. ONCOGENE, vol. 25; p. 6140-6146,
ISSN: 0950-9232
• MENSITIERI G, LAROBINA D, GUERRA G, VENDITTO V, FERMEGLIA M., PRICL S (2008). Chloroform sorption in
nanoporous crystalline and amorphous phases of syndiotactic polystyrene. JOURNAL OF POLYMER SCIENCE. PART
B, POLYMER PHYSICS, vol. 46; p. 8-15, ISSN: 0887-6266
• ZAMPIERI D, MAMOLO M.G, VIO L, BANFI E, SCIALINO G, FERMEGLIA M., FERRONE M, PRICL S. (2007).
Synthesis, antifungal and antimycobacterial activities of new bis-imidazole derivatives, and prediction of their
binding to P45014DM by molecular docking and MM/PBSA method. BIOORGANIC & MEDICINAL CHEMISTRY, vol.
15; p. 7444-7458, ISSN: 0968-0896
• MAZZEI M, NIEDDU E, MIELEA M, BALBIA, A, FERRONE M, FERMEGLIA M., MAZZEI M.T, PRICL S., LACOLLA P,
MARONGIU F, IBBA C, LODDO R (2008). Activity of Mannich bases of 7-hydroxycoumarin against Flaviviridae.
• FERMEGLIA M., LONGO G, TOMA L (2008). COWAR: A CAPE OPEN Software Module for the Evaluation of Process
Sustainability. ENVIRONMENTAL PROGRESS, vol. 27; p. 373-383, ISSN: 0278-4491
• MALY M, POSOCCO P, PRICL S, FERMEGLIA M. (2008). Self-Assembly of Nanoparticle Mixtures in Diblock
Copolymers: Multiscale Molecular Modeling. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, vol. 47; p.
5023-5038, ISSN: 0888-5885
• MALY M, POSOCCO P, FERMEGLIA M., PRICL S. (2008). Scripting approach in hybrid organic-inorganic
condensation simulation: the GPTMS proof-of-concept. MOLECULAR SIMULATION, vol. 34; p. 1215-1236, ISSN:
0892-7022
• COSOLI P, FERMEGLIA M., FERRONE M (2008). GCMC Simulations in Zeolite MFI and Activated Carbon for
Benzene Removal from Exhaust Gaseous Streams. MOLECULAR SIMULATION, vol. 34; p. 1321-1327, ISSN: 08927022
• PRICL S, PAVAN G.M, NEGRI T, FERMEGLIA M., PIEROTTI M.A, PILOTTI S, TAMBORINI E (2008). The long and
winding road of the KIT juxtamembrane (JXM) domain. ONCOGENE, ISSN: 0950-9232
• COSOLI P, FERRONE M, PRICL S., FERMEGLIA M. (2008). Hydrogen sulphide removal from biogas by zeolite
adsorption: Part I. GCMC molecular simulations. CHEMICAL ENGINEERING JOURNAL, vol. 145; p. 86-92, ISSN:
1385-8947
• COSOLI P, FERRONE M, PRICL S., FERMEGLIA M. (2008). Hydrogen sulphide removal from biogas by zeolite
adsorption-part II: a MD simulation. CHEMICAL ENGINEERING JOURNAL, vol. 145; p. 93-99, ISSN: 1385-8947
• MARTIN L, KORTABERRIA G, VAZQUEZ A, FERMEGLIA M., MARTINELLI L, SINESI S, JIMENO A, DE LA CABA K,
MONDRAGON I (2008). A comparative study of nanocomposites based on a recycled poly(methyl methacrylate)
matrix containing several nanoclays. POLYMER COMPOSITES, vol. 29; p. 782-790, ISSN: 0272-8397
• FERMEGLIA M. (2008). Hydrogen Technology: Environmental Scope. In: ALINE LEON. Hydrogen Technology",
edited by , , Berlin, D (2008). vol. 1, p. 637-654, BERLIN: Springer-Verlag, ISBN/ISSN: 3540790276
• FERMEGLIA M., LONGO G, TOMA L (2009). Computer aided design for sustainable industrial processes: specific
tools and applications. AICHE JOURNAL, vol. 55; p. 1065-1078, ISSN: 0001-1541
• ZAMPIERI D, MAMOLO MG, LAURINI E, FERMEGLIA M., POSOCCO P, PRICL S, BANFI E, SCIALINO G, VIO L
(2009). Antimycobacterial activity of new 3,5-disubstituted 1,3,4-oxadiazol-2(3H)-one derivatives. Molecular
modeling investigations. BIOORGANIC & MEDICINAL CHEMISTRY, vol. 17; p. 4693-4707, ISSN: 0968-0896
• TONELLI M, VAZZANA I, TASSO B, BOIDO V, SPARATORE F, FERMEGLIA M., PANENI M.S, POSOCCO P, PRICL S.,
LA COLLA P, IBBA C, SECCI B, COLLU G, LODDO R (2009). Antiviral and cytotoxic activities of aminoarylazo
compounds
and aryltriazene derivatives. BIOORGANIC & MEDICINAL CHEMISTRY, vol. 17; p. 4425-4440, ISSN: 0968-0896
• SCOCCHI G, POSOCCO P, HANDGRAAF J.W, FRAAIJE J.G.E.M, FERMEGLIA M., PRICL, S (2009). A complete
multiscale modelling approach for polymer-clay nanocomposites. CHEMISTRY-A EUROPEAN JOURNAL, vol. 15; p.
7586-7592, ISSN: 0947-6539
• ZAMPIERI D., M.G. MAMOLO, E. LAURINI, C. FLORIO, C. ZANETTE, FERMEGLIA M., P. POSOCCO, M.S. PANENI,
S. PRICL, L. VIO (2009). Synthesis, biological evaluation, and three-dimensional in silico pharmacophore model for
sigma1 receptor ligand based on a series of benzo[d]oxazol-2(3H)-one derivatives. JOURNAL OF MEDICINAL
CHEMISTRY, vol. 52; p. 5380-5393, ISSN: 0022-2623
• NEGRI T, PAVAN G.M, VIRDIS E, GRECO A, FERMEGLIA M., PRICL S, PIEROTTI M.A, PILOTTI S, TAMBORINI E
(2009). T670X KIT mutations in gastrointestinal stromal tumors: making sense of missense. JOURNAL OF THE
NATIONAL CANCER INSTITUTE, vol. 101; p. 194-204, ISSN: 0027-8874
• FERMEGLIA M., PRICL S (2009). Multiscale molecular modeling in nanostructured material design and process
system engineering. COMPUTERS & CHEMICAL ENGINEERING, vol. 33; p. 1701-1710, ISSN: 0098-1354
• MICHELE TONELLI, VITO BOIDO, CATERINA CANU, SPARATORE A, SPARATORE F, PANENI M.S, FERMEGLIA M.,
180
PRICL S., LA COLLA P, CASULA L, IBBA C, COLLU D, LODDO R (2008). Antimicrobial and cytotoxic
arylazoenamines. Part III: Antiviral activity of selected classes of arylazoenamines. BIOORGANIC & MEDICINAL
• ANGUSTI A, MANFREDINI S, DURINI E, CILIBERTI N, VERTUANI S, SOLAROLI N, PRICL S, FERRONE M,
FERMEGLIA M., LODDO R, SECCI B, VISIOLI A, SANNA T, COLLU G, PEZZULLO M, LA COLLA P (2008). Design,
synthesis and anti flaviviridae activity of N6-, -O- and 5 O-substituted adenine nucleoside analogs. CHEMICAL &
PHARMACEUTICAL BULLETIN, vol. 56; p. 423-432, ISSN: 0009-2363
• CARTA A, LORIGA M, PIRAS S, PAGLIETTI G, FERRONE M, FERMEGLIA M., PRICL S., COLLU G, SANNA T, LODDO
R (2007). SYNTHESIS AND ANTI-PICORNAVIRIDAE IN VITRO ACTIVITY OF A NEW CLASS OF HELICASE
INHIBITORS THE N,N’-BIS-[4-1H(2H)-BENZOTRIAZOL-1(2)-YL)PHENYL] ALKYLDICARBOXAMIDES. MEDICINAL
• E. LAURINI, D. ZAMPIERI, M.G. MAMOLO, L. VIO, C. ZANETTE, C. FLORIO, P. POSOCCO, FERMEGLIA M., S.
PRICL (2010). A 3D-Pharmacophore Model for σ2 Receptors Based on a Series of Substituted Benzo[d]oxazol2(3H)-one Derivatives. BIOORGANIC AND MEDICINAL CHEMISTRY LETTERS; p. 2954-2957, ISSN: 1464-3405
• P. COSOLI, FERMEGLIA M., M. FERRONE (2010). Molecular Simulation of Atrazine Adhesion and Diffusion in a
Saturated Sand Model. SOIL & SEDIMENT CONTAMINATION, vol. 19; p. 72-87, ISSN: 1532-0383, doi:
10.1080/15320380903390505
• CARTA A., PRICL S., PIRAS S., FERMEGLIA M., LA COLLA P., LODDO R. (2009). Activity and molecular modeling
of a new small molecule active against NNRTI-resistant HIV-1 mutants. EUROPEAN JOURNAL OF MEDICINAL
• TONELLI M., BOIDO V., LA COLLA P.L., LODDO R., POSOCCO P., PANENI M.S., FERMEGLIA M., PRICL S. (2010).
Pharmacophore modeling, resistant mutant isolation, docking, and MM-PBSA analysis: Combined
experimental/computer-assisted approaches to identify new inhibitors of the bovine viral diarrhea virus (BVDV).
• FERMEGLIA M., MALY M, POSOCCO P, PRICL S. (2009). Multiscale Molecular Modeling of Hybrid OrganicInorganic Nanocomposites of Type I and II. In: VINCENZINI P., D'ARRIGO, G.. Advances in Science and
Technology: Smart Materials & Micro/Nanosystems. vol. 54, p. 265-269, BASEL: Trans Tech Publications,
ISBN/ISSN: 3-908158-20-6
• POSOCCO P, PAVAN G, SCOCCHI G, HANDGRAAF J.W, MALEK A, MALY M, FERMEGLIA M., FRAAIJE J, CATAPANO
V, DANANI A, PRICL S. (2009). Base Invaders. Coupling Experiments and Multiscale Modeling of Dendrimer-Based
siRNA Delivery Agents. In: VINCENZINI P., DE ROSSI D.. Advances in Science and Technology: Smart Materials &
Micro/Nanosystems. vol. 57, p. 154-159, BASEL: Trans Tech Publications, ISBN/ISSN: 3-908158-20-6
• T. J. STEMKE-HALE, K. LAZAR A.J., PRICL S., PAVAN G.M., FERMEGLIA M., GOPAL Y.N.V., YANG D., PODOLOFF
D.A., IVAN D., KIM K.B., PAPADOPOULOS N., HWU P., MILLS G.B., DAVIES M.A., WOODMAN, S.E. (2009). Activity
of dasatinib against L576P KIT mutant melanoma: Molecular, cellular, and clinical correlates. MOLECULAR CANCER
THERAPEUTICS, vol. 8; p. 2079-2085, ISSN: 1535-7163
• CONCA, E., NEGRI T., GRONCHI A., FUMAGALLI E., TAMBORINI E., PAVAN G.M., FERMEGLIA M., PIEROTTI M.A.,
PRICL S., PILOTTI S. (2009). Activate and resist: L576P-KIT in GIST. MOLECULAR CANCER THERAPEUTICS, vol. 8;
p. 2491-2495, ISSN: 1535-7163
• TOTH R., VOORN D., HANDGRAAF J.-W., FRAAIJE J.G.E.M., FERMEGLIA M., PRICL S., POSOCCO P. (2009).
Multiscale computer simulation studies of water-based montmorillonite/ poly(ethylene oxide) nanocomposites.
MACROMOLECULES, vol. 42; p. 8260-8270, ISSN: 0024-9297
• MARONGIU A., LODDO R., TONELLI M., BOIDO V., LAURINI E., POSOCCO P., FERMEGLIA M., PRICL S.,
GILIBERTI G. (2010). Synergistic experimental/computational studies on arylazoenamine derivatives that target
the bovine viral diarrhea virus RNA-dependent RNA polymerase. BIOORGANIC & MEDICINAL CHEMISTRY, vol. 18;
p. 6055-6068, ISSN: 1464-3391
• TOTH R., FERMEGLIA M., PRICL S., MENSITIERI G., LAVORGNA M., PISCITELLI F. (2010). Sodium
montmorillonite silylation: Unexpected effect of the aminosilane chain length. JOURNAL OF COLLOID AND
INTERFACE SCIENCE, vol. 351; p. 108-115, ISSN: 0021-9797
• P. POSOCCO, Z. POSEL, FERMEGLIA M., M. LISAL, S. PRICL (2010). A molecular simulation approach to the
prediction of the morphology of self-assembled nanoparticles in diblock copolymers. JOURNAL OF MATERIALS
CHEMISTRY, vol. 20; p. 10511-10520, ISSN: 0959-9428, doi: 10.1039/c0jm01561j
• POSOCCO P, FERMEGLIA M., PRICL S (2010). Morphology prediction of block copolymers for drug delivery by
mesoscale simulations. JOURNAL OF MATERIALS CHEMISTRY, vol. 20; p. 7742-7753, ISSN: 0959-9428
12. FIRRAO Giuseppe
• BENEDETTI R, NAZZI F, LOCCI R, FIRRAO G. (2006). Degradation of fumonisin B1 by a bacterial strain isolated
from soil. BIODEGRADATION, vol. 17; p. 31-38, ISSN: 0923-9820
• TORELLI E, FIRRAO G., LOCCI R, GOBBI E (2006). Ochratoxin A-producing strains of Penicillium spp. isolated
from grapes used for the production of "passito" wines. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, vol.
106; p. 307-312, ISSN: 0168-1605
• FIRRAO G., GARCIA-CHAPA M, MARZACHI C (2007). Phytoplasmas: genetics, diagnosis and relationships with
the plant and insect host. FRONTIERS IN BIOSCIENCE, vol. 12; p. 1353-1375, ISSN: 1093-4715
• FIRRAO G., CONCI L, LOCCI R (2007). Molecular Identification and Diversity of Phytoplasmas. In: PUNJA Z.K.,
DE BOER S.H., SANFACON H.. Biotechnology and Plant Disease Management. p. 250-276, WALLINGFORD: CABI,
ISBN/ISSN: 9781845932886
• CETTUL E, REKAB D, LOCCI R, FIRRAO G. (2008). Evolutionary analysis of endopolygalacturonase encoding
genes of Botrytis cinerea. MOLECULAR PLANT PATHOLOGY, vol. 9; p. 675-685, ISSN: 1464-6722
• MORETTI M, DI FABRIZIO E, CABRINI S, MUSETTI R, DE ANGELIS F, FIRRAO G. (2008). An ON/OFF biosensor
based on blockade of ionic current passing through a solid state nanopore. BIOSENSORS & BIOELECTRONICS, vol.
24; p. 141-147, ISSN: 0956-5663
• TORELLI E, GUBIANI R, FIRRAO G., CIVIDINO S, LOCCI R, GOBBI E (2010). Air analysis in the assessment of
fumonisin contamination risk in maize. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, vol. 90; p. 641-
181
649, ISSN: 0022-5142
• REKAB D, PIRAJNO G, CETTUL E, DE SALVADOR F. R, FIRRAO G. (2010). On the apple proliferation symptom
display and the canopy colonization pattern of “Candidatus Phytoplasma mali” in apple trees. EUROPEAN JOURNAL
OF PLANT PATHOLOGY, vol. 127; p. 7-12, ISSN: 0929-1873
• FIRRAO G., TORELLI E, GOBBI E, RARANCIUC S, BIANCHI G, LOCCI R (2010). Prediction of milled maize
fumonisin contamination by multispectral image analysis. JOURNAL OF CEREAL SCIENCE, vol. 52; p. 327-330,
ISSN: 0733-5210
• BULL C.T, DE BOER S.H, DENNY T.P, FIRRAO G., M FISCHER-LE SAUX, M, SADDLER G.S, SCORTICHINI M,
STEAD D.E, TAKIKAWA Y (2010). Comprehensive List of Names of Plant Pathogenic Bacteria, 1980-2007. JOURNAL
OF PLANT PATHOLOGY, ISSN: 1125-4653
• PANE C, REKAB D, FIRRAO G., RUOCCO M, SCALA F (2008). A novel gene coding for an ABC transporter in
Botrytis cinerea (Botryotinia fuckeliana) is involved in resistance to H2O2. JOURNAL OF PLANT PATHOLOGY, vol.
90; p. 453-462, ISSN: 1125-4653
• BULL C.T, DE BOER, S.H, DENNY T.P, FIRRAO G., FISCHER-LE SAUX M, SADDLER G.S, SCORTICHINI M, STEAD
D.E, TAKIKAWA Y (2008). Demystifying the nomenclature of bacterial plant pathogens. JOURNAL OF PLANT
PATHOLOGY, vol. 90; p. 403-417, ISSN: 1125-4653
13. FORNASIERO Paolo
• ALESSANDRI I, BANARES M.A, DEPERO L.E, FERRONI M, FORNASIERO P., GENNARI F.C, HICKEY N., MARTINEZHUERTA M.V, MONTINI T (2006). Structural investigation of Ce2Zr2O8 after redox treatments which lead to low
temperature reduction. TOPICS IN CATALYSIS, vol. 41 (1-4); p. 35-42, ISSN: 1022-5528, doi: 10.1007/S11244006-0092-8
• MONTINI T, CONDO' AM, HICKEY N, LOVEY F.C, DE ROGATIS L, FORNASIERO P., GRAZIANI M (2007).
Embedded Rh(1wt %)@Al2O3: Effects of high temperature and prolonged aging under methane partial oxidation
conditions. APPLIED CATALYSIS. B, ENVIRONMENTAL, vol. 73; p. 84-97, ISSN: 0926-3373, doi:
10.1016/j.apcatb.2006.06.008
• GENNARI F.C, MONTINI T, HICKEY N, FORNASIERO P., GRAZIANI M (2006). IR investigation of the interaction of
deuterium with Ce0.6Zr0.4O2 and Cl-doped Ce0.6Zr0.4O2. APPLIED SURFACE SCIENCE, vol. 252; p. 8456-8465,
ISSN: 0169-4332, doi: 10.1016/J.APSUSC.2005.11.062
• GENNARI F.C, NEYERTZ C, MAYER G, MONTINI T, FORNASIERO P. (2006). Hydrogen adsorption kinetics on
Pd/Ce0.8Zr0.2O2. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, vol. 8; p. 1-11, ISSN: 1463-9076
• SHAH P.R, KIM T, ZHOU G, FORNASIERO P., GORTE R.J (2006). Evidence for entropy effects in the reduction of
ceria-zirconia solutions. CHEMISTRY OF MATERIALS, vol. 18; p. 5363-5369, ISSN: 0897-4756
• BEVILACCQUA M, MONTINI T, TAVAGNACCO C, VICARIO G, FORNASIERO P., GRAZIANI M (2006). Influence of
synthesis route on morphology and electrical properties of LaNi0.6Fe0.4O3. SOLID STATE IONICS, vol. 177; p.
2957-2965, ISSN: 0167-2738
• MONTINI T, DE ROGATIS L, GOMBAC V, FORNASIERO P., GRAZIANI M (2007). Rh(1%)@CexZr1-xO2-Al2O3
nanocomposites: active and stable catalysts for Ethanol Steam Reforming. APPLIED CATALYSIS. B,
ENVIRONMENTAL, vol. 71; p. 125-134, ISSN: 0926-3373
• HICKEY N, FORNASIERO P., GRAZIANI M (2006). Hydrogen based technologies for mobile applications.
Renewable Resources and Renewable Energy: A Global Challenge. p. 225-272, NEW YORK: CRC Taylor and Francis,
ISBN/ISSN: 978-0-8493-9689-2
• ZHOU G, SHAH P.R, KIM T, FORNASIERO P., GORTE R.J (2007). Oxidation entropies and enthalpies of ceria–
zirconia solid solutions. CATALYSIS TODAY, vol. 123; p. 86-93, ISSN: 0920-5861
• HICKEY N, LAROCHETTE P. A, GENTILINI C, SORDELLI L, OLIVI L, POLIZZI S, MONTINI T, FORNASIERO P.,
PASQUATO L., GRAZIANI M (2007). Monolayer protected gold nanoparticles on ceria for an efficient CO oxidation
catalyst. CHEMISTRY OF MATERIALS, vol. 19; p. 650-651, ISSN: 0897-4756
• GOMBAC V, DE ROGATIS L, GASPAROTTO A, VICARIO G, MONTINI T, BARRECA D, BALDUCCI G, FORNASIERO
P., TONDELLO E, GRAZIANI M. (2007). TiO2 nanopowders doped with boron and nitrogen for photocatalytic
applications. CHEMICAL PHYSICS, vol. 339; p. 111-123, ISSN: 0301-0104
• BETTINELLI M, DALLACASA V, FALCOMER D, FORNASIERO P., GOMBAC V, MONTINI T, ROMAN L, SPEGHINI A
(2007). Photocatalytic activity of TiO2 doped with boron and vanadium. JOURNAL OF HAZARDOUS MATERIALS, vol.
146; p. 529-534, ISSN: 0304-3894, doi: 10.1016/J.JHAZMAT.2007.04.053
• DE ROGATIS L, MONTINI T, CASULA MF, FORNASIERO P. (2008). Design of [email protected]
nanocomposite for ethanol steam reforming. JOURNAL OF ALLOYS AND COMPOUNDS, vol. 451; p. 516-520, ISSN:
0925-8388, doi: 10.1016/j.jallcom.2007.04.231
• MONTINI T, SPEGHINI A, FORNASIERO P., BETTINELLI M, GRAZIANI M (2008). Effect of the thermal
pretreatments on ceria – zirconia redox properties: an Eu3+ luminescence study. JOURNAL OF ALLOYS AND
COMPOUNDS, vol. 451; p. 617-620, ISSN: 0925-8388, doi: 10.1016/j.jallcom.2007.04.074
• ZHOU G, SHAH P.R, MONTINI T, FORNASIERO P., GORTE R.J (2007). Oxidation Enthalpies for Reduction of Ceria
Surfaces. SURFACE SCIENCE, vol. 601; p. 2512-2519, ISSN: 0039-6028
• FORNASIERO P., MONTINI T, GRAZIANI M, ZILLIO S, SUCCI M (2008). Development of Catalytic Nanoceramicloaded Fe-Al-Cr Alloy Fiber Media for Exhaust Gas Purification. CATALYSIS TODAY, vol. 137; p. 475-482, ISSN:
0920-5861, doi: 10.1016/j.cattod.2007.12.097.
• FORNASIERO P., MONTINI T, DE ROGATIS L (2007). Catalysts Design for Hydrogen Production: Embedded
Rhodium Nanoparticles. Diffusion and Reactivity of Solids. vol. -, p. 65-109, NEW YORK: Nova Science Publisher,
ISBN/ISSN: 1-60021-890-3
• ZINOVIEV S, FORNASIERO P., LODOLO A, MIERTUS S (2007). Non-combustion thechnologies for POPs
destruction: review and evaluation. TRIESTE: ICS-UNIDO
• FAHMY Y.M, FORNASIERO P., ZINOVIEV S, MIERTUS S (2007). Air Pollution Control Thechnologies:
Compendium. TRIESTE: ICS-UNIDO
• BEVILACQUA M, MONTINI T, TAVAGNACCO C, FONDA E, FORNASIERO P., GRAZIANI M (2007). Preparation,
characterization and electrochemical properties of pure and composite LaNi0.6Fe0.4O3 – based cathodes for IT-
182
SOFC. CHEMISTRY OF MATERIALS, vol. 19; p. 5926-5936, ISSN: 0897-4756, doi: 10.1021/cm071622n
• DING X, DE ROGATIS L, VESSELLI E, BARALDI A, COMELLI G, ROSEI R, SAVIO L, VATTUONE L, ROCCA M,
FORNASIERO P., ANCILOTTO F, BALDERESCHI A, PERESSI M. (2007). Interaction of carbon dioxide with Ni(110): a
• VESSELLI E, DE ROGATIS L, DING X, BARALDI A, SAVIO L, VATTUONE L, ROCCA M, FORNASIERO P., PERESSI
M, BALDERESCHI A, ROSEI R, AND COMELLI G (2008). Hydrogen-assisted Carbon Dioxide Activation on Ni(110).
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 130; p. 11417-11422, ISSN: 0002-7863, doi:
10.1021/ja802554g.
• NATILE M.M, POLETTO F, GALENDA A, GLISENTI A, MONTINI T, DE ROGATIS T, FORNASIERO P. (2008).
La0.6Sr0.4Co1-yFeyO3-d perovskites: influence of the Co/Fe atomic ratio on properties and catalytic activity
towards alcohol steam-reforming. CHEMISTRY OF MATERIALS, vol. 20; p. 2314-2327, ISSN: 0897-4756, doi:
10.1021/cm703329k.
• GENNARI F.C, MONTINI M, FORNASIERO P., ANDRADE GAMBOA J.J (2008). REDUCTION BEHAVIOR OF
NANOPARTICLES OF Ce0.8Zr0.2O2 PRODUCED BY DIFFERENT APPROACHES. INTERNATIONAL JOURNAL OF
HYDROGEN ENERGY, vol. 33; p. 3549-3554, ISSN: 0360-3199, doi: 10.1016/j.ijhydene.2007.12.006.
• FITTIPALDI M, GOMBAC V, MONTINI T, FORNASIERO P., GRAZIANI M (2008). A High-Frequency (95 GHz)
Electron Paramagnetic Resonance Study of B-doped TiO2 photocatalysts. INORGANICA CHIMICA ACTA, vol. 361; p.
3980-3987, ISSN: 0020-1693, doi: 10.1016/j.ica.2008.03.052
• BERT P, CATANORCHI S, FORNASIERO P., GRAZIANI M, LORENZUT B, MILLER H, MONTINI T, RAGNOLI M,
SCAFFIDI A, TAMPUCCI A (2007). Catalizzatori per la produzione di gas di sintesi da reforming di
idrocarburi o alcoli comprendenti un supporto in ZnO e loro uso. FI2007A0000179. Acta spa
• DE ROGATIS L, MONTINI T, COGNIGNI A, OLIVI L, FORNASIERO P. (2009). Methane Partial Oxidation on NiCubased catalysts. CATALYSIS TODAY, vol. 145; p. 176-185, ISSN: 0920-5861, doi: 10.1016/j.cattod.2008.04.019
• ROSSIN A, IENCO A, COSTANTINO F, MONTINI, T, DI CREDICO B, CAPORALI M, GONSALVI L, FORNASIERO P.,
PERUZZINI M (2008). Crystal-to-crystal phase transition and CO2 adsorption properties of polymeric magnesium
formate. CRYSTAL GROWTH & DESIGN, vol. 8; p. 3302-3308, ISSN: 1528-7483
• DE ROGATIS L, MONTINI T, GOMBAC V, FORNASIERO P. (2008). Stabilized metal nanoparticles embedded into
porous oxides: a challenging approach for robust catalysts. In: WESLEY V. PRESCOTT AND ARNOLD I. SCHWARTZ
EDITORS. Nanorods, Nanotubes and Nanomaterials Research Progress. vol. -, p. 71-123, NEW YORK: Nova Science
Publisher, ISBN/ISSN: 978-1-60456-122-7
• DE ROGATIS L, MONTINI T, LORENZUT B, FORNASIERO P. (2008). NixCuy/Al2O3 based catalysts for hydrogen
production. ENERGY & ENVIRONMENTAL SCIENCE, vol. 1; p. 509-509, ISSN: 1754-5692, doi: 10.1039/B805426F
• HAMEED A, MONTINI T, GOMBAC V, FORNASIERO P. (2008). Surface Phases and Photocatalytic Activity
Correlation of Bi2O3/Bi2O4-x Nanocomposite. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 130; p. 96589659, ISSN: 0002-7863
• HAMEED A, MONTINI T, GOMBAC. V, FORNASIERO P. (2009). Photocatalytic decolourization of dyes on NiO–ZnO
nano-composites. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, vol. 8; p. 677-682, ISSN: 1474-905X, doi:
10.1039/b817396f
• HAMEED A, GOMBAC V, MONTINI T, GRAZIANI M, FORNASIERO P. (2009). Synthesis, Characterization and
Photocatalytic Activity of NiO-Bi2O3 Nanocomposites. CHEMICAL PHYSICS LETTERS, vol. 472; p. 212-216, ISSN:
0009-2614, doi: 10.1016/j.cplett.2009.03.017
• BARRECA D, FORNASIERO P., GASPAROTTO A, GOMBAC V, MACCATO C, MONTINI T, TONDELLO E (2009). The
Potential of Supported Cu2O and CuO Nanosystems in Photocatalytic H2 Production. CHEMSUSCHEM, vol. 2; p.
230-233, ISSN: 1864-5631, doi: 10.1002/cssc.200900032
• GOMBAC V, MONTINI T, POLIZZI S, DELGADO- JAÉN J.J, HAMEED A, FORNASIERO P. (2009). Photocatalytic
Production of Hydrogen over Tailored Cu-Embedded TiO2. NANOSCIENCE AND NANOTECHNOLOGY LETTERS, vol.
1; p. 128-133, ISSN: 1941-4900, doi: 10.1166/nnl.2009.1017
• MONTINI T, BEVILACQUA M, FONDA E, CASULA M.F, LEE S, TAVAGNACCO C, GORTE R.J, FORNASIERO P.
(2009). Relationship between electrical behavior and structural characteristics in Sr-doped LaNi0.6Fe0.4O3-d
mixed oxides. CHEMISTRY OF MATERIALS, vol. 21; p. 1768-1774, ISSN: 0897-4756, doi: 10.1021/cm900467c
• SIVASAMY A, CHEAH K.Y, FORNASIERO P., KEMAUSUOR F, ZINOVIEV S, MIERTUS S (2009). Catalytic
Applications in Biodiesel Production from Vegetable Oils: A Review. CHEMSUSCHEM, vol. 2; p. 278-300, ISSN:
1864-5631, doi: 10.1002/cssc.200800253
• LEE S, BEVILACQUA M, FORNASIERO P., VOHS J. M, R.J. GORTE (2009). SOFC Cathodes Prepared by Infiltration
of LNF and LSNF in Porous YSZ. JOURNAL OF POWER SOURCES, vol. 193; p. 747-753, ISSN: 0378-7753, doi:
10.1016/j.jpowsour.2009.04.046
• GASPAROTTO A, BARRECA D, FORNASIERO P., GOMBAC V, LEBEDEV O.I, MACCATO C, MONTINI T, TONDELLO
E, VAN TENDELOO G, COMINI E, SBERVEGLIERI G (2009). Multi-functional copper oxide nanosystems for H2
sustainable production and sensing. ECS TRANSACTIONS, vol. 25; p. 1169-1176, ISSN: 1938-5862, doi:
10.1149/1.3207721
• DE ROGATIS L, FORNASIERO P. (2009). Catalysts design for reforming of oxygenates. In: BARBARO P,
BIANCHINI C. EDITORS. Catalysis for Sustainable Energy Production. p. 173-233, WEINHEIM: WILEY-VCH,
ISBN/ISSN: 978-3-52732095-0
• MONTINI T, SPEGHINI A, DE ROGATIS L, LORENZUT B, BETTINELLI M, GRAZIANI M, FORNASIERO P. (2009).
The identification of the structural phases of CexZr1-xO2 by Eu(III) luminescence studies. JOURNAL OF THE
AMERICAN CHEMICAL SOCIETY, vol. 131; p. 13155-13160, ISSN: 0002-7863, doi: 10.1021/ja905158p
• DE ROGATIS L, VESSELLI E, BARALDI A, CASULA M.F, MONTINI T, COMELLI G, GRAZIANI M, FORNASIERO P.
• VESSELLI E, RIZZI M, DE ROGATIS L, DING X, BARALDI A, COMELLI G, SAVIO L, VATTUONE L, ROCCA M,
FORNASIERO P., BALDERESCHI A, PERESSI M (2010). Hydrogen-assisted transformation of CO2 on nickel: the role
of formate and carbon monoxide. THE JOURNAL OF PHYSICAL CHEMISTRY LETTERS, vol. 1; p. 402-406, ISSN:
183
1948-7185, doi: 10.1021/jz900221c.
• CARGNELLO M, WIEDER N. L, MONTINI T, GORTE R.J, FORNASIERO P. (2010). Synthesis of dispersible
Pd@CeO2 nanostructures by self-assembly. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 132; p. 14021409, ISSN: 0002-7863, doi: 10.1021/JA909131K
• STEFANIA SPECCHIA, LUIGI D. VELLA, BARBARA LORENZUT, TIZIANO MONTINI, VITO SPECCHIA, FORNASIERO
P. (2010). Effect of the catalyst load on syngas production in SCT-CPO reactors. INDUSTRIAL & ENGINEERING
CHEMISTRY RESEARCH, vol. 49; p. 1010-1017, ISSN: 0888-5885, doi: 10.1021/ie900695j
• VALENTINA GOMBAC, LAURA SORDELLI, TIZIANO MONTINI, JUAN J. DELGADO, ADRZEJ ADAMSKI, GIANPIERO
ADAMI, MATTEO CARGNELLO, SERAFIN BERNAL, FORNASIERO P. (2010). CuOx−TiO2 Photocatalysts for H2
Production from Ethanol and Glycerol Solutions. JOURNAL OF PHYSICAL CHEMISTRY. A, MOLECULES,
SPECTROSCOPY, KINETICS, ENVIRONMENT, & GENERAL THEORY, vol. 114; p. 3916-3925, ISSN: 1089-5639, doi:
10.1021/jp907242q
• MATTEO CARGNELLO, TIZIANO MONTINI, STEFANO POLIZZI, NOAH L. WIEDER, RAYMOND J. GORTE, MAURO
GRAZIANI, FORNASIERO P. (2010). Novel embedded Pd@CeO2 catalysts: a way to active and stable catalysts.
DALTON TRANSACTIONS, vol. 39; p. 2122-2127, ISSN: 1477-9234, doi: 10.1039/b916035c
• DE ROGATIS L, CARGNELLO M, GOMBAC V, LORENZUT B, MONTINI T., FORNASIERO P. (2010). Embedded
phases: a way to active and stable catalysts. CHEMSUSCHEM, vol. 3; p. 24-42, ISSN: 1864-5631, doi:
10.1002/cssc.200900151
• LORENZUT B., MONTINI T., PAVEL C.C., COMOTTI M., VIZZA F., BIANCHINI C., FORNASIERO P. (2010).
Embedded Ru@ZrO2 catalysts for H2 production by ammonia decomposition. CHEMCATCHEM, vol. 2; p. 10961106, ISSN: 1867-3880, doi: 10.1002/cctc.201000097
• SPECCHIA S., VELLA L. D., DE ROGATIS L., MONTINI T., SPECCHIA V., FORNASIERO P. (2010). Rh-based
Catalysts for Syngas Production via SCT-CPO Reactors. CATALYSIS TODAY, vol. 155; p. 101-107, ISSN: 09205861, doi: 10.1016/j.cattod.2009.04.008
• MONTINI T., GOMBAC V., HAMEED A., FELISARI L., ADAMI G., FORNASIERO P. (2010). Synthesis,
characterization and photocatalytic performance of transition metal tungstates. CHEMICAL PHYSICS LETTERS, vol.
498; p. 113-119, ISSN: 0009-2614, doi: 10.1016/j.cplett.2010.08.026
• ZINOVIEV S., MULLER-LANGER F., DAS P., BERTERO N., FORNASIERO P., KALTSCHMITT M., CENTI G., MIERTUS
S. (2010). Next generation biofuels: survey of emerging technologies and sustainability issues. CHEMSUSCHEM,
vol. 3; p. 1106-1133, ISSN: 1864-5631, doi: 10.1002/cssc.201000052
• LORENZUT B., MONTINI T., DE ROGATIS L., CANTON P., BENEDETTI A., FORNASIERO P. (2011). Hydrogen
production through alcohol steam reforming on Cu/ZnO-based catalysts. APPLIED CATALYSIS. B,
ENVIRONMENTAL, vol. 101; p. 397-408, ISSN: 0926-3373, doi: 10.1016/j.apcatb.2010.10.009
• BARRECA D., FORNASIERO P., GASPAROTTO A., GOMBAC V., MACCATO C., POZZA A., TONDELLO E. (2010).
CVD Co3O4 nanopyramids: a nano-platform for the photo-assisted H2 production. CHEMICAL VAPOR DEPOSITION,
vol. 16; p. 296-300, ISSN: 0948-1907, doi: 10.1002/cvde.201004289
• WIEDER N.L., CARGNELLO M., BAKHMUTSKY K., MONTINI T., FORNASIERO P., GORTEAND R.J. (2011). A study
of the water-gas-shift reaction over Pd@CeO2/Al2O3 core-shell catalysts. JOURNAL OF PHYSICAL CHEMISTRY. C,
NANOMATERIALS AND INTERFACES, vol. 115; p. 915-919, ISSN: 1932-7447
• MONTINI T., GOMBAC V., SORDELLI L., DELGADO J.J., CHEN X., ADAMI G., FORNASIERO P. (2011).
Nanostructured Cu/TiO2 photocatalysts for H2 production from ethanol and glycerol aqueous solutions.
CHEMCATCHEM, vol. 3; p. 574-577, ISSN: 1867-3880, doi: 10.1002/cctc.201000289
• KIM J.S., WIEDER N.L., ABRAHAM A.J., CARGNELLO M., FORNASIERO P., GORTE R.J., VOHS J.M. (2011). Highly
Active and Thermally Stable Core-Shell Catalysts for Solid Oxide Fuel Cells. JOURNAL OF THE ELECTROCHEMICAL
SOCIETY, vol. 158; p. B596-B600, ISSN: 0013-4651, doi: 10.1149/1.3571039
• GIAMBASTIANI G., CICCHI S., ZOPPI M., GIANNASI A., LUCONI L., ROSSIN A., BIANCHINI C., MERCURI F.,
BRANDI A., MELUCCI M., GHINI G., STAGNARO P., CONZATTI L., PASSAGLIA E., MONTINI T., FORNASIERO P.
(2011). Functionalization of Multiwalled Carbon Nanotubes with Cyclic Nitrones for Materials and Composites:
Addressing the Role of CNT Sidewall Defects. CHEMISTRY OF MATERIALS, vol. 23; p. 1923-1938, ISSN: 08974756, doi: 10.1021/cm103655y
• D. VELLA L., MONTINI T., SPECCHIA S, FORNASIERO P. (2011). Fixed beds of Rh/Al2O3-based catalysts for
syngas production in methane SCT-CPO reactors. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, vol. 36; p.
7776-7784, ISSN: 0360-3199, doi: 10.1016/j.ijhydene.2011.01.183
• GENNARI F.C., RAMOS A.C., CONDÓ A., MONTINI T., BENGIÓ S., CORTESI A., ANDRADE-GAMBOA J.J.,
FORNASIERO P. (2011). Hydrogen Interaction with Pd/Ce0.8Zr0.2O2 Nanocomposites Prepared by Microemulsion,
Coprecipitation and Supercritical CO2 Treatment. APPLIED CATALYSIS A: GENERAL, vol. 398; p. 123-133, ISSN:
0926-860X, doi: 10.1016/j.apcata.2011.03.022
• BARRECA D., CARRARO G., GOMBAC V., GASPAROTTO A., MACCATO C., FORNASIERO P., TONDELLO E. (2011).
Supported metal oxide nanosystems for H2 photo-generation: quo vadis?. ADVANCED FUNCTIONAL MATERIALS,
vol. 21; p. 2611-2623, ISSN: 1616-301X, doi: 10.1002/adfm.201100242
14. FRONZONI Giovanna
• FRONZONI G., DE FRANCESCO R, STENER M. (2007). TDDFT calculations of NEXAFS spectra of model systems
for SO2 adsorbed on the MgO (100) surface. JOURNAL OF PHYSICAL CHEMISTRY. C, NANOMATERIALS AND
INTERFACES, vol. 111; p. 13554-13563, ISSN: 1932-7447
• M. STENER, A. NARDELLI, R. DE FRANCESCO, FRONZONI G. (2007). Optical excitations of gold nanoparticles: a
quantum chemical scalar relativistic Time Dependent Density Functional study. JOURNAL OF PHYSICAL
CHEMISTRY. C, NANOMATERIALS AND INTERFACES, vol. 111; p. 11862-11871, ISSN: 1932-7447, doi:
10.1021/jp072712i
• M. STENER, D. TOFFOLI, FRONZONI G., P. DECLEVA (2007). Recent advances in molecular photoionization by
density functional theory based approaches. THEORETICAL CHEMISTRY ACCOUNTS, vol. 117; p. 943-956, ISSN:
1432-881X
• FRONZONI G., DE FRANCESCO R, STENER M., DECLEVA P (2007). Spin-Orbit Relativistic calculations of the core
184
excitation spectra of SO2. THE JOURNAL OF CHEMICAL PHYSICS, vol. 126; p. 134308 1-10, ISSN: 0021-9606
• J. DURAND, E. ZANGRANDO, M. STENER, FRONZONI G., C. CARFAGNA, B. BINOTTI, P. C. J. KAMER, C. MULLER,
M. CAPORALI, P. W. N. M. VAN LEEUWEN, D. VOGT, B. MILANI (2006). Long-Lived Palladium Catalysts for CO/Vinyl
Arene Polyketones Synthesis: A Solution to Deactivation Problems. CHEMISTRY-A EUROPEAN JOURNAL, vol. 12; p.
7639-7651, ISSN: 0947-6539
• R. DE FRANCESCO, M. STENER, M. CAUSÀ, D. TOFFOLI, FRONZONI G. (2006). Time Dependent Density
Functional investigation of the near-edge absorption spectra of V2O5. PHYSICAL CHEMISTRY CHEMICAL PHYSICS,
vol. 8; p. 4300-4310, ISSN: 1463-9076
• D. TOFFOLI, M. STENER, FRONZONI G., DECLEVA P. (2006). Photoionization Cross Section and angular
Distribution Calculations on Carbon Tetrafluoride. THE JOURNAL OF CHEMICAL PHYSICS, vol. 124; p. 214313-,
ISSN: 0021-9606
• FRONZONI G., DE FRANCESCO R, STENER M., CAUSA' M (2006). X-Ray absorption spectroscopy of titanium
oxide by Time Dependent Density Functional calculations. JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED
MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL, vol. 110; p. 9899-9907, ISSN: 1520-6106
• D. DI TOMMASO, M. STENER, FRONZONI G., DECLEVA P. (2006). Conformational effects on Circular Dichroism in
the photoelectron angular distribution. CHEMPHYSCHEM, vol. 7; p. 924-, ISSN: 1439-4235
• STENER M., TOFFOLI D, FRONZONI G., DECLEVA P (2006). Time Dependent Density Functional study of the
photoionization dynamics of SF6. THE JOURNAL OF CHEMICAL PHYSICS, vol. 124; p. 114306 1-13, ISSN: 00219606
• STENER M., DI TOMMASO D., FRONZONI G., DECLEVA P., POWIS I. (2006). Theoretical study on the circular
dichroism in core and valence photoelectron angular distributions of camphor enantiomers. THE JOURNAL OF
CHEMICAL PHYSICS, vol. 124; p. 024326 1-10, ISSN: 0021-9606, doi: 10.1063/1.2150438
• M. STENER, A. NARDELLI, FRONZONI G. (2008). Theoretical study on the photoabsorption of MAul2- (M = V, Nb,
Ta). CHEMICAL PHYSICS LETTERS, vol. 462; p. 358-364, ISSN: 0009-2614
• A. KIVIMÄKI, J. ÁLVAREZ RUIZ, M. CORENO, M. STANKIEWICZ, FRONZONI G., DECLEVA P. (2008).
Photoelectron spectroscopy of sulfur L levels in the SF5CF3 molecule. CHEMICAL PHYSICS, vol. 353; p. 202-,
ISSN: 0301-0104
• DE FRANCESCO R, STENER M., FRONZONI G. (2009). S K-edge NEXAFS Spectra of Model Systems for SO2 on
TiO2(110) : a TDDFT Simulation. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, vol. 11; p. 1146-1151, ISSN: 14639076, doi: 10.1039/b811925b
• CAUSÀ M, BARONE V, STENER M., FRONZONI G. (2008). Electrostatic effects on cluster simulation of ionic
crystals and surfaces. JOURNAL OF PHYSICS. CONFERENCE SERIES, vol. 117; p. 012009 1-8, ISSN: 1742-6596
• M. STENER, A. NARDELLI, FRONZONI G. (2008). Spin-orbit effects in the photoabsorption of WAu12 and
MoAu12: a relativistic Time Dependent Density Functional study. THE JOURNAL OF CHEMICAL PHYSICS, vol. 128;
p. 134307-, ISSN: 0021-9606
• FRONZONI G., STENER M., DECLEVA P., DE SIMONE M, CORENO M, FRANCESCHI P, FURLANI C, PRINCE K. C
(2009). X-Ray absorption spectroscopy of VOCl3, CrO2Cl2 and MnO3Cl: an experimental and theoretical study.
JOURNAL OF PHYSICAL CHEMISTRY. A, MOLECULES, SPECTROSCOPY, KINETICS, ENVIRONMENT, & GENERAL
THEORY, vol. 113; p. 2914-2925, ISSN: 1089-5639, doi: 10.1021/jp808720z
• DECLEVA P., FRONZONI G., KIVIMÄKI A, ÁLVAREZ RUIZ J, SVENSSON S (2009). Shake-up transitions in S 2p, S
2s and F 1s photoionization of the SF6 molecule. JOURNAL OF PHYSICS. B, ATOMIC MOLECULAR AND OPTICAL
PHYSICS, vol. 42; p. 055102-, ISSN: 0953-4075
• STENER M., FRONZONI G., DECLEVA P. (2009). Time Dependent Density Functional Theory description of giant
resonances in transition metal complexes: the photoionization dynamics of Cr(CO)6. CHEMICAL PHYSICS, vol. 361;
p. 49-60, ISSN: 0301-0104, doi: 10.1016/j.chemphys.2009.05.006
• KIVIMÄKI A., ÁLVAREZ RUIZ J., CORENO M., STANKIEWICZ M., FRONZONI G., STENER M., DECLEVA P. (2010).
S 2p photoabsorption of the SF5CF3 molecule: experiment, theory and comparison
with SF6. CHEMICAL PHYSICS, vol. 375; p. 101-109, ISSN: 0301-0104, doi: 10.1016/j.chemphys.2010.07.031
• KORICA S., REINKÖSTER A., BRAUNE M., VIEFHAUS J., ROLLES D., LANGER B., FRONZONI G., TOFFOLI D.,
STENER M., DECLEVA P., AL-DOSSARY O. M., BECKER U. (2010). Partial photoionization cross sections of C60 and
C70: a gas versus adsorbed phase comparison. SURFACE SCIENCE, vol. 604; p. 1940-1944, ISSN: 0039-6028,
doi: 10.1016/j.susc.2010.07.031
• NARDELLI A., FRONZONI G., STENER M. (2009). Theoretical study on the X-Ray absorption at the sulphur Kedge in gold nanoparticles protected by thiolates. JOURNAL OF PHYSICAL CHEMISTRY. C, NANOMATERIALS AND
INTERFACES, vol. 113; p. 14844-14851, ISSN: 1932-7447
• NARDELLI A., FRONZONI G., STENER M. (2011). Theoretical study of sulphur L-edge XANES of thiol protected
gold nanoparticles. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, vol. 13; p. 480-487, ISSN: 1463-9076, doi:
10.1039/c0cp00708k
• DE FRANCESCO R., STENER M., FRONZONI G. (2011). Computational investigation of the L2,3-edge spectra of
bulk and (110) surface of rutile TiO2. SURFACE SCIENCE, vol. 605; p. 500-506, ISSN: 0039-6028, doi:
10.1016/j.susc.2010.12.006
• STENER M., BOLOGNESI P., CORENO M., O'KEEFFE P., FEYER V., FRONZONI G., DECLEVA P., AVALDI L.,
KIVIMÄKI A. (2011). Photoabsorption and S 2p photoionization of the SF6 molecule: resonances in the excitation
energy range of 200-280 eV. THE JOURNAL OF CHEMICAL PHYSICS, vol. 134; p. 174311 1-174311 9, ISSN: 00219606, doi: 10.1063/1.3583815
15. GAMINI Amelia
• ROSSI M, CAMPA C, GAMINI A., COSLOVI A, DONATI I, VETERE A, PAOLETTI S. (2006). Separation of O- and Callyl glycoside anomeric mixtures by capillary electrophoresis and high-performance liquid chromatography.
JOURNAL OF CHROMATOGRAPHY A, vol. 1110; p. 125-132, ISSN: 0021-9673
• GAMINI A., A. COSLOVI, I. RUSTIGHI, C. CAMPA, A. VETERE, S. PAOLETTI (2008). Use of capillary
electrophoresis for polysaccharide studies and applications. In: P. SCHMITT-KOPLIN ED., HUMANA PRESS INC..
Capillary electrophoresis. Methods and Protocols
185
Series: Methods in Molecular Biology ,. vol. 384, p. 357-400, NEUHERBERG/MÜNCHEN: P. SCHMITT-KOPLIN,
ISBN/ISSN: 978-1-58829-539-2, doi: 10.1007/978-1-59745-376-9_13
• CESARO A, GAMINI A., DE GIACOMO O, MASCIOVECCHIO C, GESSINI A, DI FONZO S (2007). Study of
longitudinal dynamics of trehalose-water solutions by inelastic ultraviolet scattering. PHILOSOPHICAL MAGAZINE,
vol. 87; p. 623-630, ISSN: 1478-6435
• RUSTIGHI I., CAMPA C., ROSSI M., SEMERARO S., VETERE A., GAMINI A. (2009). Analysis of Nacetylaminosugars by CE: A Comparative Derivatization Study. ELECTROPHORESIS, vol. 30; p. 2632-2639, ISSN:
0173-0835
16. GIRALDI Tullio
• STOCCO G, MARTELOSSI S, SARTOR F, TOFFOLI G, LIONETTI P, BARABINO A, FONTANA M, DECORTI G.,
BARTOLI F, GIRALDI T., VENTURA A (2006). Prevalence of methylenetetrahydrofolate reductase polymorphisms in
young patients with inflammatory bowel disease. DIGESTIVE DISEASES AND SCIENCES, vol. 51; p. 474-479,
ISSN: 0163-2116
• GIRALDI T., DE VANNA M., MALAGOLI M, TUVERI G, SUTTO K, SCHILLANI G, GRASSI L (2007). Mental
adaptation to cancer: depression and blood platelet monoamine oxidase activity in breast cancer patients.
ANTICANCER RESEARCH, vol. 27(3b); p. 1715-1719, ISSN: 0250-7005
• GIULIA SCHILLANI, MARIA ANNA CAPOZZO, EUGENIO AGUGLIA, MAURIZIO DE VANNA, LUIGI GRASSI, MARIA
ANNA CONTE, GIRALDI T. (2008). 5-HTTLPR POLYMORPHISM OF SEROTONIN TRANSPORTER AND EFFECTS OF
SERTRALINE IN TERMINALLY ILL CANCER PATIENTS: REPORT OF ELEVEN CASES. TUMORI, vol. 94; p. 563-567,
ISSN: 0300-8916
• CAPOZZO MA, SCHILLANI G, AGUGLIA E., DE VANNA M, GRASSI L, CONTE MA, GIRALDI T. (2009). Serotonin
transporter 5-HTTLPR polymorphism and response to citalopram in terminally ill cancer patients: report of twentyone cases. TUMORI, vol. 95; p. 479-483, ISSN: 0300-8916
• SCHILLANI G, GOLJEVSCEK S., CARLINO D., DE VANNA M., AGUGLIA E., GIRALDI T. (2009). Repeated suicidal
behaviour: Stressful life events and 5-HTTLPR genetic
polymorphism. INTERNATIONAL JOURNAL OF PSYCHIATRY IN CLINICAL PRACTICE, vol. 13(3); p. 229-232, ISSN:
1365-1501, doi: 10.1080/13651500902785652
• CAPOZZO MA, MARTINIS E, PELLIS G, GIRALDI T. (2009). An early structured psychoeducational intervention in
patients with breast cancer. CANCER NURSING, ISSN: 0162-220X
• L. GRASSI, E. ROSSI, M. COBIANCHI, L. AGUIARI, M. CAPOZZO, E. MARTINIS, M. G. NANNI, G. LELLI, G.
SCHILLANI, B. BIANCOSINO, GIRALDI T. (2010). Depression and serotonin transporter (5-HTTLPR) polymorphism
in breast cancer patients. JOURNAL OF AFFECTIVE DISORDERS, vol. 124; p. 346-350, ISSN: 0165-0327, doi:
10.1016/j.jad.2009.12.022
• GIULIA SCHILLANI, MARIA ANNA CAPOZZO, DANIEL ERA, MAURIZIO DE VANNA, LUIGI GRASSI, MARIA ANNA
CONTE, GIRALDI T. (in stampa). PHARMACOGENETIC OF ESCITALOPRAM AND MENTAL ADAPTATION TO CANCER
IN PALLIATIVE CARE: REPORT OF 18 CASES. TUMORI; p. XX-XX, ISSN: 0300-8916
• SCHILLANI G, MARTINIS E, CAPOZZO MA, ERA D, CRISTANTE T, MUSTACCHI G, CONTE MA, DE VANNA M,
GRASSI L, GIRALDI T. (2010). Psychological Response to Cancer: Role of 5-HTTLPR Genetic Polymorphism of
Serotonin Transporter. ANTICANCER RESEARCH; p. 3823-3826, ISSN: 0250-7005
17. LUGHI Vanni
• LUGHI V., CLARKE DR (2007). Low-temperature transformation kinetics of electron-beam deposited 5 wt.%
yttria-stabilized zirconia. ACTA MATERIALIA, vol. 55; p. 2049, ISSN: 1359-6454
• LUGHI V., MASSI PAVAN A, QUAIA S, SULLIGOI G (2008). Economical analysis and innovative solutions for grid
connected PV plants. In: SPEEDAM 2008 - International Symposium on Power Electronics, Electrical Drives,
Automation and Motion, p. 211
• LUGHI V., FERLUGA A, SERGO V, ROITTI S (2008). Crescita per bagno chimico e caratterizzazione di film sottili
di CdS per applicazioni fotovoltaiche. In: Atti del 9° convegno nazionale AIMAT, p. 425
• LUGHI V., CLARKE DR (2007). Temperature dependence of the yttria-stabilized zirconia Raman spectrum.
JOURNAL OF APPLIED PHYSICS, vol. 101; p. 053524, ISSN: 0021-8979
• LUGHI V., CLARKE DR (2007). Defect and stress characterization of AlN films by Raman spectroscopy. APPLIED
PHYSICS LETTERS, vol. 89; p. 241911, ISSN: 0003-6951
• CALLAGHAN LA, LUGHI V., MACDONALD NC, CLARKE DR (2006). Beam-supported AlN thin film bulk acoustic
resonators. IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL, vol. 53; p.
1001, ISSN: 0885-3010
• GENTLEMAN MM, LUGHI V., NYCHKA JA, CLARKE DR (2006). Noncontact methods for measuring thermal barrier
coating temperatures. INTERNATIONAL JOURNAL OF APPLIED CERAMIC TECHNOLOGY, vol. 3; p. 105, ISSN: 1546542X
• LUGHI V. (2009). Fisica della conversione fotovoltaica. Manuale di Energia Solare. Tecniche Nuove
• FAULHABER S, KRAEMER S, LUGHI V., CLARKE DR, LEVI CG, HUTCHINSON JW, EVANS AG (2007). Mechanisms
of Delamination in
Thermal Barrier Systems Subject to Calcium-Magnesium-Alumino-Silicate (CMAS) Penetration. In: Proceedings of
the 31st international
conference on advanced ceramics and composites. Daytona Beach FL
• LUGHI V. (2008). Method for the manufacturing of photovoltaic material. PCT/IB2008/054783
• QUAIA S., LUGHI V., MASSI PAVAN A, SULLIGOI G (2008). Economical analysis and innovative solutions for grid
connected PV plants. In: a. Ischia (Italy), 11-13 June, 2008, C: b, p. 211-216, ISBN/ISSN: 978-1-4244-1664-6
• SLEJKO E., RADIVO A., ANTONIOLLI F., LUGHI V. (2010). Equilibrium size maps and novel aspects of the growth
kinetics of CdSe nanocrystals, p. --• COZZARINI L., DELBEN M., RADIVO A., ANTONIOLLI F., LUGHI V. (2010). Optical and structural properties of
CdSe/ZnSe core-shell nanocrystals for novel colloidal materials, p. --• LUGHI V. (in stampa). Optical and electronic properties of nanostructures and nanomaterials. In: Bharat
Bhushan. Encyclopedia of Nanotechnology. p. ---, Springer Verlag, ISBN/ISSN: 0000000000
186
• LUGHI V. (in stampa). Surface Energy and Chemical Potential at the Nanoscale. In: Bharat Bhushan.
Encyclopedia of Nanotechnology. p. ---, Springer Verlag, ISBN/ISSN: 0000000000
18. MARSI Stefano
• GUARNIERI G., MARSI S., GIOVANNI RAMPONI (2006). Fast bilateral filter for edge-preserving smoothing.
ELECTRONICS LETTERS, vol. 42(7); p. 396-397, ISSN: 0013-5194
• S. SAPONARA, L. FANUCCI, MARSI S., G. RAMPONI, M. WITTE, D. KAMMLER (2006). Design of Application
Specific Instruction-set Processor for Image and Video Filtering. In: EUSIPCO-06, 14th European Signal Processing
Conf. Florence, Italy, 4-8 Sept.
• MARSI S., RAFFIN MARIO IT, CARRAI PAOLA IN (2007). A FILTER FOR RETRACTING A LOCAL ADAPTATION
LUMINANCE. WO2007026304. KONINKL PHILIPS ELECTRONICS NV (NL); MARSI STEFANO (IT); RAFFIN MARIO
(IT); CARRAI PAOLA (IN)
• MARSI S., IMPOCO G, UKOVICH A, CARRATO S, RAMPONI G (2007). Video Enhancement and Dynamic range
Control of HDR Sequences for Automotive Applications. EURASIP JOURNAL ON ADVANCES IN SIGNAL
PROCESSING, vol. Volume 2007; p. 80971-1-80971-9, ISSN: 1687-6172, doi: 10.1155/2007/80971
• SAPONARA S, FANUCCI L, MARSI S., RAMPONI G, KAMMLER D, WITTE EM (2007). Application-Specific
Instruction-Set Processor for Retinex-Like Image and Video Processing. IEEE TRANSACTIONS ON CIRCUITS AND
SYSTEMS. II, EXPRESS BRIEFS, vol. 54(7); p. 596-600, ISSN: 1549-7747
• S. SAPONARA, L. FANUCCI, MARSI S., RAMPONI G. (2007). Algorithmic and Architectural Design for Real-time
and Power-efficient Retinex Image/Video Processing. JOURNAL OF REAL-TIME IMAGE PROCESSING, vol. 1(4); p.
267-283, ISSN: 1861-8200, doi: 10.1007/S11554-007-0027-Z
• MARSI S., IMPOCO G, UKOVICH A, RAMPONI G, CARRATO S (2008). Using a Recursive Rational Filter to Enhance
Color Images. IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT, vol. 57(6); p. 1230-1236, ISSN:
0018-9456, doi: 10.1109/TIM.2007.915141
• MARSI S., SAPONARA S. (2010). Integrated video motion estimator with Retinex-like pre-processing for robust
motion analysis in automotive scenarios: algorithmic and real-time architecture design. JOURNAL OF REAL-TIME
IMAGE PROCESSING, vol. 2010; p. 275-290, ISSN: 1861-8200
• GUARNIERI G., MARSI S., RAMPONI G. (2011). High dynamic range image display with halo and clipping
prevention. IEEE TRANSACTIONS ON IMAGE PROCESSING, vol. 20(5); p. 1351-1362, ISSN: 1057-7149, doi:
10.1109/TIP.2010.2092436
19. MARZARI Roberto
• SBLATTERO D., VENTURA A., TOMMASINI, A., CATTIN L., MARTELOSSI, S., FLORIAN F., MARZARI R., R.,
BRADBURY A., NOT. T. (2006). Cryptic gluten intolerance in type 1 diabetes: identifying suitable candidates for
gluten-free diet. GUT, vol. 55; p. 133-134, ISSN: 0017-5749
• MARZARI R., SBLATTERO D (2006). "Recombinant antibodies against human type ii transglutaminase and uses
thereof". WO2006100679. Quark Biotech Inc.
• FISCHETTI F, DURIGUTTO P, MACOR P, MARZARI R., CARRETTA R, TEDESCO F. (2007). Selective therapeutic
control of C5a and the terminal complement complex by anti-C5 single-chain Fv in an experimental model of
antigen-induced arthritis in rats. ARTHRITIS AND RHEUMATISM, vol. 56(4); p. 1187-1197, ISSN: 0004-3591
• BARONE, M. V, I. CAPUTO, M. T. RIBECCO, M. MAGLIO, MARZARI R., D. SBLATTERO, R. TRONCONE, S.
AURICCHIO, AND C. ESPOSITO (2007). Humoral immune response to tissue transglutaminase is related to
epithelial cell proliferation in celiac disease. GASTROENTEROLOGY, vol. 132; p. 1245-1253, ISSN: 0016-5085
• E. AZZONI, D. SBLATTERO, M. LICCIULLI, MARZARI R., EDOMI P. (2007). Using archaeal histones for precise
DNA fragmentation , 20: 267-271 (2007). PROTEIN ENGINEERING, DESIGN & SELECTION, vol. 20; p. 267-271,
ISSN: 1741-0126
• DI NIRO R, SBLATTERO D, FLORIAN F, STEBEL M, ZENTILIN L, GIACCA M, VILLANACCI V, GALLETTI A, NOT T.,
VENTURA A, MARZARI R. (2008). Anti-idiotypic response in mice expressing human autoantibodies. MOLECULAR
IMMUNOLOGY, vol. 45(6); p. 1782-1791, ISSN: 0161-5890
• BOSCOLO S, SARICH A, LORENZON A, PASSONI M, RUI V, STEBEL M, SBLATTERO D, MARZARI R., TONGIORGI
E. (2007). Gluten ataxia: passive transfer in a mouse model. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES,
vol. 1107; p. 319-328, ISSN: 0077-8923
• DI NIRO, R, F. ZILLER, F. FLORIAN, S. CROVELLA, M. STEBEL, M. BESTAGNO, O. BURRONE, A. R. BRADBURY, P.
SECCO, MARZARI R., AND D. SBLATTERO (2007). Construction of miniantibodies for the in vivo study of human
autoimmune diseases in animal models. BMC BIOTECHNOLOGY, vol. 7(1); p. 46-56, ISSN: 1472-6750
• NEMEC G, VENTURA A., MARTELOSSI S, DI LEO G, BALDAS V, TOMMASINI A, FERRARA F, TADDIO A, CITTA A,
SBLATTERO D, MARZARI R., NOT T (2006). Looking for celiac disease: diagnostic accuracy of two rapid commercial
assays. AMERICAN JOURNAL OF GASTROENTEROLOGY, vol. 101(7); p. 1597-1600, ISSN: 0002-9270
• MACOR P, TRIPODO C, ZORZET S., PIOVAN E, BOSSI F, MARZARI R., AMADORI A, TEDESCO F (2007). In vivo
targeting of human neutralizing antibodies against CD55 and CD59 to lymphoma cells increases the antitumor
activity of rituximab. CANCER RESEARCH, vol. 67; p. 10556-10563, ISSN: 0008-5472
• MARZARI R., SBLATTERO DANIELE, TEDESCO FRANCESCO (2008). RECOMBINANT ANTIBODIES AGAINST CD55
AND CD59 AND USES THEREOF. WO2006IL00521 20060501; US20050677752P 20050504; US20050699024P
20050713; US20050733950P 20051103. QUARK PHARMACEUTICALS INC (US)
• SECCO P, E. D'AGOSTINI, MARZARI R., M. LICCIULLI, R.DI NIRO, S. D'ANGELO, A.R. BRADBURY, U. DIANZANI,
C. SANTORO, D. SBLATTERO (2009). Antibody library selection by the {beta}-lactamase protein fragment
complementation assay. PROTEIN ENGINEERING, DESIGN & SELECTION, ISSN: 1741-0126
• SECCHIERO P, SBLATTERO D, CHIARUTTINI C, MELLONI E, MACOR P, ZORZET S, TRIPODO C, TEDESCO F,
MARZARI R., ZAULI G (2009). Selection and characterization of a novel agonistic human recombinant anti-TRAILR2 minibody with anti-leukemic activity. INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY,
vol. 22; p. 73-83, ISSN: 0394-6320
• MAGLIO M, FLORIAN F, VECCHIET M, AURICCHIO R, PAPARO F, SPADARO R, ZANZI D, RAPACCIUOLO L,
FRANZESE A, SBLATTERO D, MARZARI R., TRONCONE R (2009). Majority of children with type 1 diabetes produce
and deposit anti-tissue transglutaminase antibodies in the small intestine. DIABETES, vol. 58; p. 1758-1784, ISSN:
187
0012-1797
• DI NIRO R, DANGELO S, SECCO P, MARZARI R., SANTORO C, SBLATTERO D (2009). Profiling the Autoantibody
Repertoire by Screening Phage-Displayed Human cDNA Libraries. METHODS IN MOLECULAR BIOLOGY, vol. 570; p.
353-369, ISSN: 1064-3745
• CAJA, S., E. MYRSKY, I. R. KORPONAY-SZABO, C. NADALUTTI, A. M. SULIC, M. LAVRIC, D. SBLATTERO,
MARZARI R., R. COLLIGHAN, A. MONGEOT, M. GRIFFIN, M. MAKI, K. KAUKINEN, AND K. LINDFORS (2010).
Inhibition of transglutaminase 2 enzymatic activity ameliorates the anti-angiogenic effects of coeliac disease
autoantibodies. SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, vol. 45; p. 421-427, ISSN: 0036-5521
• R. DI NIRO, A. M. SULIC, F. MIGNONE, S. D'ANGELO, R. BORDONI, M. IACONO, MARZARI R., T. GAIOTTO, M.
LAVRIC, A. R. BRADBURY, L. BIANCONE, D. ZEVIN-SONKIN, G. DE BELLIS, C. SANTORO, AND D. SBLATTERO
(2010). Rapid interactome profiling by massive sequencing. NUCLEIC ACIDS RESEARCH; p. "-"-"-", ISSN: 03051048
• ZAULI G., CORALLINI F., ZORZET S., GRILL V., MARZARI R., SECCHIERO P. (2010). In vivo anti-lymphoma
activity of an agonistic human recombinant anti-TRAIL-R2 minibody. INVESTIGATIONAL NEW DRUGS; p. ---, ISSN:
0167-6997
• BOSCOLO S, LORENZON A, SBLATTERO D, FLORIAN F, STEBEL M, MARZARI R., NOT T, AESCHLIMANN D,
VENTURA A, HADJIVASSILIOU M, TONGIORGI E (2010). Anti transglutaminase antibodies cause ataxia in mice.
PLOS ONE, vol. 15;5(3); p. e9698-e9698, ISSN: 1932-6203
20. MORGANTE Alberto
• L. FLOREANO, A. COSSARO, D. CVETKO, G. BAVDEK, A. MORGANTE (2006). Phase diagram of pentacene growth
on Au(110)". JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES
& BIOPHYSICAL, vol. 110; p. 4908, ISSN: 1520-6106
• CUDIA CC, VILMERCATI P, LARCIPRETE R, CEPEK C, ZAMPIERI G, SANGALETTI L, PAGLIARA S, VERDINI A,
COSSARO A, FLOREANO L, A. MORGANTE, PETACCIA L, LIZZIT S, BATTOCCHIO C, POLZONETTI G, GOLDONI A
(2006). Electronic structure and molecular orientation of a Zn-tetra-phenyl porphyrin multilayer on Si(111).
SURFACE SCIENCE, vol. 600; p. 4013-4017, ISSN: 0039-6028
• VILMERCATI P, CUDIA CC, LARCIPRETE R, CEPEK C, ZAMPIERI G, SANGALETTI L, PAGLIARA S, VERDINI A,
COSSARO A, FLOREANO L, A. MORGANTE, PETACCIA L, LIZZIT S, BATTOCCHIO C, POLZONETTI G, GOLDONI A
(2006). Molecular orientations, electronic properties and charge transfer timescale in a Zn-porphyrin/C-70 donoracceptor complex for solar cells. SURFACE SCIENCE, vol. 600; p. 4018-4023, ISSN: 0039-6028
• VERDINI A, COSSARO A, FLOREANO L, A. MORGANTE, GOLDONI A, GHIDONI D, SEPE A, PAGLIARA S,
SANGALETTI L (2006). Surface and electronic properties of the Mn : Ge(111) interface at the early stages of
growth. SURFACE SCIENCE, vol. 600; p. 4369-4374, ISSN: 0039-6028
• R. MAZZARELLO, A. COSSARO, A. VERDINI, R. ROUSSEAU, L. CASALIS, M. F. DANISMAN, L. FLOREANO, S.
SCANDOLO, A. MORGANTE, AND G. SCOLES (2007). Structure of a CH3S Monolayer on Au(111) Solved by the
Interplay between Molecular Dynamics Calculations and Diffraction Measurements. PHYSICAL REVIEW LETTERS,
vol. 98; p. 016102, ISSN: 0031-9007
• ALBANO COSSARO, SILVANA TERRENI, ORNELLA CAVALLERI, MIRKO PRATO, DEAN CVETKO, A. MORGANTE,
LUCA FLOREANO, AND MAURIZIO CANEPA (2006). Electronic and Geometric Characterization of the L-Cysteine
Paired-Row Phase on Au(110). LANGMUIR, vol. 22; p. 11193-11198, ISSN: 0743-7463
• A. SCHIFFRIN, A. RIEMANN, W. AUWARTER, Y. PENNEC, A. WEBER-BARGIONI, D. CVETKO, A. COSSARO, A.
MORGANTE, JV. BARTH (2007). Zwitterionic self-assembly of L-methionine nanogratings on the Ag(111) surface.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 104; p.
5279, ISSN: 0027-8424
• P. KRUEGER, S. BOURGEOIS, B. DOMENICHINI, H. MAGNAN, D. CHANDESRIS, P. LE FEVRE, L. FLOREANO, A.
COSSARO, A. VERDINI, A. MORGANTE (2007). Defects at the TiO2 surface probed by resonant photoelectron
diffraction. SURFACE SCIENCE, vol. 601; p. 3952, ISSN: 0039-6028
• E. SALOMON, N. PAPAGEORGIOU, T. ANGOT, A. VERDINI, A. COSSARO, L. FLOREANO, A. MORGANTE, L.
GIOVANELLI, AND G. LE LAY (2007). Lead-phtalocyanine films studied by NearEdge X-ray Absorption Fine
Spectroscopy. JOURNAL OF PHYSICAL CHEMISTRY. C, NANOMATERIALS AND INTERFACES, vol. 111; p. 12467,
ISSN: 1932-7447
• G. BAVDEK, A. COSSARO, D. CVETKO, C. AFRICH, C. BLASETTI, F. ESCH, A. MORGANTE, L. FLOREANO (2008).
Pentacene nanorails on Au(110). LANGMUIR, vol. 24; p. 767, ISSN: 0743-7463
• A. VERDINI, A. COSSARO, L. FLOREANO, A. MORGANTE, A. GOLDONI, D. GHIDONI, A. SEPE, S. PAGLIARA, L.
SANGALETTI (2008). Local coordination of Mn atoms at the Mn:Ge(111) interface from photoelectron diffraction
experiments. PHYSICAL REVIEW. B, CONDENSED MATTER AND MATERIALS PHYSICS, vol. 77; p. 075405, ISSN:
1098-0121
• P. KRUEGER, S. BOURGEOIS, B. DOMENICHINI, H. MAGNAN, D. CHANDESRIS, P. LE FEVRE, A.M. FLANK, J.
JUPILLE, L. FLOREANO, A. COSSARO, A. VERDINI, A. MORGANTE (2008). Defect states at the TiO2(110) surface
probed by resonant photoelectron diffraction. PHYSICAL REVIEW LETTERS, vol. 100; p. 055501, ISSN: 0031-9007
• M. CHIODI, L. GAVIOLI, M. BECCARI, V. DI CASTRO, A. COSSARO, L. FLOREANO, A. MORGANTE, A. KANJILAL,
C. MARIANI, M.G.BETTI (2008). Interaction strength and molecular orientation of a single layer of pentacene in
organic-metal interface and organic-organic heterostructure. PHYSICAL REVIEW. B, CONDENSED MATTER AND
MATERIALS PHYSICS, vol. 77; p. 115321, ISSN: 1098-0121
• L. FLOREANO, A. COSSARO, R. GOTTER, A. VERDINI, G. BAVDEK, F. EVANGELISTA, A. RUOCCO, A. MORGANTE,
D. CVETKO (2008). Periodic arrays of Cu-Phthalocyanine chains on Au(110. JOURNAL OF PHYSICAL CHEMISTRY. C,
NANOMATERIALS AND INTERFACES, vol. 112; p. 10794, ISSN: 1932-7447
• A. COSSARO, R. MAZZARELLO, R. ROUSSEAU, L. CASALIS, A. VERDINI, A. KOHLMEYER, L. FLOREANO, S.
SCANDOLO, A. MORGANTE, M. L. KLEIN AND G. SCOLES (2008). X-ray Diffraction and Computation Yield Structure
of Alkanethiols on Au(111). SCIENCE, vol. 321; p. 943-946, ISSN: 0036-8075
• K. R. KNOX, S. WANG, A. MORGANTE, D. CVETKO, A. LOCATELLI, T. O. MENTES, M. A. NIÑO, P. KIM, AND R. M.
OSGOOD, JR (2008). Spectromicroscopy of single and multilayer graphene supported by a weakly interacting
188
substrate. PHYSICAL REVIEW. B, CONDENSED MATTER AND MATERIALS PHYSICS, vol. 78; p. 201408, ISSN:
1098-0121
• P. VILMERCATI, C. CASTELLARIN-CUDIA, R. GEBAUER, P. GHOSH, S. LIZZIT, L. PETACCIA, C. CEPEK, R.
LARCIPRETE, A. VERDINI, L. FLOREANO, A. MORGANTE, A. GOLDONI (2009). Mesoscopic Donor−Acceptor
Multilayer by Ultrahigh-Vacuum Codeposition of Zn-Tetraphenyl-Porphyrin and C70. JOURNAL OF THE AMERICAN
CHEMICAL SOCIETY, vol. 131; p. 644-652, ISSN: 0002-7863
• P. VILMERCATI, D. CVETKO, A. COSSARO, A. MORGANTE (2009). Heterostructured organic interfaces probed by
resonant photoemission. SURFACE SCIENCE, vol. 603; p. 1542-1556, ISSN: 0039-6028
• S.D. THORPE, F. ARCIPRETE, E. PLACIDI, F. PATELLA, M. FANFONI, A. BALZAROTTI, S. COLONNA, F. RONCI, A.
CRICENTI, A. VERDINI, L. FLOREANO, A. MORGANTE (2009). XPS and STM study of Mn incorporation on the
GaAs(001) surface. SUPERLATTICES AND MICROSTRUCTURES, vol. 46; p. 258-265, ISSN: 0749-6036
• A. COSSARO, L. FLOREANO, A. VERDINI, L. CASALIS, A. MORGANTE (2009). Comment on "local methylthiolate
adsorption geometry on au(111) from photoemission core-level shifts". PHYSICAL REVIEW LETTERS, vol. 103; p.
119601, ISSN: 0031-9007
• A. SCHIFFRIN, J. REICHERT, Y. PENNEC, W. AUWRTER, A. WEBER-BARGIONI, M. MARSCHALL, M. DELLANGELA,
D. CVETKO, G. BAVDEK, A. COSSARO, A. MORGANTE, J.V. BARTH (2009). Self-Assembly of L-Methionine on
Cu(111): steering chiral organization by substrate reactivity and thermal activation. JOURNAL OF PHYSICAL
CHEMISTRY. C, NANOMATERIALS AND INTERFACES, vol. 113; p. 12101-12108, ISSN: 1932-7447
• D. G. DE OTEYZA, J. M. GARCÍA-LASTRA, M. CORSO, B. P. DOYLE, L. FLOREANO, A. MORGANTE, Y. WAKAYAMA,
A. RUBIO, J. ENRIQUE ORTEGA (2009). Customized Electronic Coupling in Self-Assembled Donor-Acceptor
Nanostructures (p NA). ADVANCED FUNCTIONAL MATERIALS, ISSN: 1616-301X, doi: 10.1002/adfm.200901374
• F. EVANGELISTA, A. RUOCCO, R. GOTTER, A. COSSARO, L. FLOREANO, A. MORGANTE, F. CRISPOLDI, M.G.
BETTI, C. MARIANI (2009). Electronic states of CuPc chains on the Au(110) surface. THE JOURNAL OF CHEMICAL
PHYSICS, vol. 131; p. 174710, ISSN: 0021-9606
• H. MAGNAN, P. LE FÈVRE, D. CHANDESRIS, P. KRÜGER, S. BOURGEOIS, B. DOMENICHINI, A. VERDINI, L.
FLOREANO, A. MORGANTE (2010). Resonant photoelectron and photoelectron diffraction across the Fe L3 edge of
Fe3O4. PHYSICAL REVIEW. B, CONDENSED MATTER AND MATERIALS PHYSICS, vol. 81; p. 085121-085121, ISSN:
1098-0121
• JOACHIM REICHERT, AGUSTIN SCHIFFRIN, WILLI AUWRTER, ALEXANDER WEBER-BARGIONI, MATTHIAS
MARSCHALL, MARTINA DELL’, ANGELA, DEAN CVETKO, GREGOR BAVDEK, ALBANO COSSARO, A. MORGANTE,
JOHANNES V. BARTH (2010). l-Tyrosine on Ag(111): Universality of the Amino Acid 2D Zwitterionic Bonding
Scheme?. ACS NANO, vol. 2010, 4 (2); p. 1218-1226, ISSN: 1936-0851, doi: 10.1021/nn901669p
• SANGALETTI L., MOZZATI M. C., DRERA G., AGUEKIAN V., FLOREANO L., A. MORGANTE, GOLDONI A.,
KARCZEWSKI G. (2010). Local electronic properties and magnetism of (Cd,Mn)Te quantum wells. APPLIED
PHYSICS LETTERS, vol. 96; p. 142105-142105, ISSN: 0003-6951, doi: 10.1063/1.3378811
• AUWARTER W., SEUFERT K., KLAPPENBERGER F., REICHERT J., WEBER-BARGIONI A., VERDINI A., CVETKO D.,
DELL'ANGELA M., FLOREANO L., COSSARO A., BAVDEK G., A. MORGANTE, SEITSONEN A.P., BARTH J.V. (2010).
Site-specific electronic and geometric interface structure of Co-tetraphenyl-porphyrin layers on Ag(111). PHYSICAL
REVIEW. B, CONDENSED MATTER AND MATERIALS PHYSICS, vol. 81; p. 245403-245403, ISSN: 1098-0121, doi:
10.1103/PhysRevB.81.245403
• SANGALETTI L., VERDINI A., PAGLIARA S., DRERA G., FLOREANO L., GOLDONI A., A. MORGANTE (2010). Local
order and hybridization effects for Mn ions probed by resonant soft x-ray spectroscopies: The Mn: CdTe(110)
interface revisited. PHYSICAL REVIEW. B, CONDENSED MATTER AND MATERIALS PHYSICS, vol. 81; p. 245320245320, ISSN: 1098-0121, doi: 10.1103/PhysRevB.81.245320
• LOCATELLI A., KNOX K. R., CVETKO D., MENTES T.O., NINO M. A., WANG S., YILMAZ M. B., KIM P., OSGOOD R.
M. JR., A. MORGANTE (2010). Corrugation in Exfoliated Graphene: An Electron Microscopy and Diffraction Study.
ACS NANO, vol. 4; p. 4879-4889, ISSN: 1936-0851, doi: 10.1021/nn101116n
• DELL’, ANGELA M., KLADNIK G., COSSARO A., VERDINI A., KAMENETSKA M., TAMBLYN I., QUEK S. Y., J. B.
NEATON J. B., CVETKO D., A. MORGANTE, VENKATARAMAN L. (2010). Relating Energy Level Alignment and AmineLinked Single Molecule Junction Conductance. NANO LETTERS, vol. 10; p. 2470-2474, ISSN: 1530-6984, doi:
10.1021/nl100817h
• JIA Z., LEE V.W., KYMISSIS I., FLOREANO L., VERDINI A., COSSARO A., A. MORGANTE (2010). In situ study of
pentacene interaction with archetypal hybrid contacts: Fluorinated versus alkane thiols on gold. PHYSICAL REVIEW.
B, CONDENSED MATTER AND MATERIALS PHYSICS, vol. 82; p. 125457-125457, ISSN: 1098-0121
• CASTELLARIN-CUDIA C., BORGHETTI P., DI SANTO G., FANETTI M., LARCIPRETE R., CEPEK C., VILMERCATI P.,
SANGALETTI L., VERDINI A., COSSARO A., FLOREANO L., A. MORGANTE, GOLDONI A. (2010). Substrate Influence
for the Zn-tetraphenyl-porphyrin Adsorption Geometry and the Interface-Induced Electron Transfer.
CHEMPHYSCHEM, vol. 11; p. 2248-2255, ISSN: 1439-4235
21. MUSCIA Roberto
• MUSCIA R. (2008). An Analytical Numerical Procedure to Define Motion Equations From Experimental Simulation
Data. In: Proceedings of the TCNCAE 2008. Venice - Italy, 16-17 October 2008, TRENTO: EnginSoft, p. 1-8
• MUSCIA R., SPERANDIO A (2008). Cad/Cae Methodology for Drum Shaft Design of Industrial Washing Machines.
In: Proceedings of TCNCAE 2008. Venice - Italy, 16-17 October 2008, TRENTO: EnginSoft, p. 1-5
• MUSCIA R. (2008). A Methodology for Hybrid Modelizations of Intracoronary Stents. In: Proceedings of the
TCNCAE 2008. Venice - Italy, 16-17 October 2008, TRENTO: EnginSoft, p. 1-4
• MUSCIA R. (2008). Analytical Numerical Computation of Magnetostatic Fields Produced by Helicoidal Magnets.
In: Proceedings of TCNCAE 2008. Venice - Italy, 16-17 October 2008, TRENTO: EnginSoft, p. 1-4
• MUSCIA R. (2008). Equivalent magnetic charge in helicoidal magnets. JOURNAL OF APPLIED PHYSICS, vol. 104;
p. 1-24, ISSN: 0021-8979, doi: 10.1063/1.2975154
• MUSCIA R. (2009). Calcolo del campo magnetico generato da un serpentino torico magnetizzato. In: Atti del
Mathematica Italia User Group Meeting 2009. Università di Padova, 24-25 Settembre 2009, AREZZO: ADALTA, p.
1-14
189
• MUSCIA R., SCIUTO G (2009). Analisi dinamica di fattibilità concettuale di un dispositivo per la propulsione
navale senza uso di eliche. In: Atti del Mathematica Italia User Group Meeting 2009. Università di Padova, 24-25
Settembre 2009, AREZZO: ADALTA, p. 1-12
• MUSCIA R., SCIUTO G (2009). A Method for Performing Quality Comparisons of Rzeppa Joints. In: Proceedings
of the 13th International Research/Expert Conference "Trends in the Development of Machinery and Associated
Technology" (TMT2009). Hammamet, Tunisia, 16-21 October, ZENICA: Ekinovic S., Calvet J. V.,Yalcin S., vol. 1, p.
241-244, ISBN/ISSN: 1840-4944
• SCIUTO G, MUSCIA R. (2009). Multiobjective Optimizations of Intracoronary Stents by Genetic Algorithm. In:
Proceedings of the 13th International Research/Expert Conference "Trends in the Development of Machinery and
Associated Technology" (TMT2009). Hammamet, Tunisia, 16-21 October 2009, ZENICA: Ekinovic S., Calvet J.
V.,Yalcin S., vol. 1, p. 597-600, ISBN/ISSN: 1840-4944
• MUSCIA R., CAMPAGNOLO F, FRANCO F (2009). A Finite Elements Procedure for Evaluating Injectors Reliability
of Medium Speed Diesel Engines. In: Proceedings of the 13th International Research/Expert Conference "Trends in
the Development of Machinery and Associated Technology" (TMT2009). Hammamet, Tunisia, 16-21 October 2009,
ZENICA: Ekinovic S., Calvet J. V.,Yalcin S., vol. 1, p. 765-768, ISBN/ISSN: 1840-4944
• MUSCIA R., SCIUTO G. (2010). Analytic study of a new conceptual propulsion device for ships. INTERNATIONAL
JOURNAL OF NAVAL ARCHITECTURE AND OCEAN ENGINEERING, vol. Volume 2, NUmber 2, June 2010; p. 75-86,
ISSN: 2092-6782
• SCIUTO G., MUSCIA R. (2010). Innovative latent heat thermal storage elements design based on
nanotecnologies. In: Proceedings of Spring College on Computational Nanoscience 2010 - Trieste. Trieste, 17-28
May 2010, Trieste: The Abdus Salam International Centre for Theoretic, p. ---, ISBN/ISSN: 0000000000
22. PAOLETTI Sergio
• DONATI I, CESARO A, PAOLETTI S. (2006). Specific Interactions versus Counterion Condensation. 1. Nongelling
Ions/Polyuronate Systems. BIOMACROMOLECULES, vol. 7(1); p. 281-287, ISSN: 1525-7797, doi:
10.1021/BM050646P
• DONATI I, BENEGAS JC, CESARO A, PAOLETTI S. (2006). Specific interactions versus counterion condensation.
2. Theoretical treatment within the counterion condensation theory. BIOMACROMOLECULES, vol. 7 (5); p. 15871596, ISSN: 1525-7797, doi: 10.1021/bm050981d
• DONATI I, BENEGAS JC, PAOLETTI S. (2006). Polyelectrolyte study of the calcium-induced chain association of
pectate. BIOMACROMOLECULES, vol. 7 (12); p. 3439-3447, ISSN: 1525-7797, doi: 10.1021/bm060164t
• DELBEN F, PAOLETTI S., PORASSO RD, BENEGAS JC (2006). Potentiometric titrations of maleic acid copolymers
in dilute aqueous solution: Experimental results and theoretical interpretation. MACROMOLECULAR CHEMISTRY
AND PHYSICS, vol. 207 (24); p. 2299-2310, ISSN: 1022-1352, doi: 10.1002/macp.200600479
• ROSSI M, CAMPA C, GAMINI A, COSLOVI A, DONATI I, VETERE A, PAOLETTI S. (2006). Separation of O- and Callyl glycoside anomeric mixtures by capillary electrophoresis and high-performance liquid chromatography.
JOURNAL OF CHROMATOGRAPHY A, vol. 1110; p. 125-132, ISSN: 0021-9673
• ABBALLE F, TOPPAZZINI M, CAMPA C, UGGERI F, PAOLETTI S. (2007). Study of molar response of dextrans in
electrochemical detection. JOURNAL OF CHROMATOGRAPHY A, vol. 1149; p. 38-45, ISSN: 0021-9673, doi:
10.1016/j.molcatb.2007.01.005
• DONATI I, HAUG IJ, SCARPA T, BORGOGNA M, DRAGET KI, SKJAK-BRAEK G, PAOLETTI S. (2007). Synergistic
effects in semi-dilute mixed solutions of alginate and lactose-modified chitosan (chitlac). BIOMACROMOLECULES,
vol. 8; p. 957-962, ISSN: 1525-7797
• COSLOVI A, CAMPA C, FLAMIGNI A, ROSSI M, VETERE A, UGGERI F, PAOLETTI S. (2007). Enzymatic synthesis of
the Tn antigen. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, vol. 45; p. 128-134, ISSN: 1381-1177, doi:
10.1016/J.MOLCATB.2007.01.005
• MARSICH E, BORGOGNA M, DONATI I, MOZETIC P, STRAND BL, SALVADOR SG, VITTUR F, PAOLETTI S. (2008).
Alginate/lactose-modified chitosan hydrogels: a bioactive biomaterial for chondrocyte encapsulation. JOURNAL OF
BIOMEDICAL MATERIALS RESEARCH. PART A, vol. 84; p. 364-376, ISSN: 1549-3296, doi: 10.1002/JBM.A.31307
• DONATI I., BORGOGNA M, TURELLO E, CESÀRO A, PAOLETTI S. (2007). Tuning supramolecular structuring at
the nanoscale level : Nonstoichiometric soluble complexes in dilute mixed solutions of alginate and lactosemodified chitosan (chitlac). BIOMACROMOLECULES, vol. 8(5); p. 1471-1479, ISSN: 1525-7797, doi:
10.1021/BM0610828
• MARSICH E, MOZETIC P, ORTOLANI F, CONTIN M, MARCHINI M, VETERE A, PACOR S, SEMERARO S, VITTUR F,
PAOLETTI S. (2008). Galectin-1 in cartilage: expression, influence on chondrocyte growth and interaction with ECM
components. MATRIX BIOLOGY, vol. 27; p. 513-525, ISSN: 0945-053X, doi: 10.1016/j.matbio.2008.04.003
• GAMINI A., A. COSLOVI, I. RUSTIGHI, C. CAMPA, A. VETERE, PAOLETTI S. (2008). Use of capillary
electrophoresis for polysaccharide studies and applications. In: P. SCHMITT-KOPLIN ED., HUMANA PRESS INC..
Capillary electrophoresis. Methods and Protocols
Series: Methods in Molecular Biology ,. vol. 384, p. 357-400, NEUHERBERG/MÜNCHEN: P. SCHMITT-KOPLIN,
ISBN/ISSN: 978-1-58829-539-2, doi: 10.1007/978-1-59745-376-9_13
• JERMAN B, BREZNIK M, KOGEJ K, PAOLETTI S. (2007). Osmotic and volume properties of stereoregular
poly(methacrylic acids) in aqueous solution: role of intermolecular association. JOURNAL OF PHYSICAL
CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL, vol. 111; p. 84358443, ISSN: 1520-6106, doi: 10.1021/jp0676080
• TURCO G, MARSICH E, BELLOMO F, SEMERARO S, DONATI I, BRUN F, GRANDOLFO M, ACCARDO A, PAOLETTI S.
(2009). Alginate/Hydroxyapatite Biocomposite For Bone Ingrowth: A Trabecular Structure With High And Isotropic
Connectivity. BIOMACROMOLECULES, vol. 10(6); p. 1575-1583, ISSN: 1525-7797, doi: 10.1021/bm900154b
• DONATI I., TRAVAN A, PELILLO C, SCARPA T, COSLOVI A, BONIFACIO A, SERGO V, PAOLETTI S. (2009). Polyol
Synthesis of Silver Nanoparticles: Mechanism of Reduction by Alditol Bearing Polysaccharides.
BIOMACROMOLECULES, vol. 10(2); p. 210-213, ISSN: 1525-7797, doi: 10.1021/BM801253C
• TRAVAN A, PELILLO C, DONATI I, MARSICH E, BENINCASA M, SCARPA T, SEMERARO S, TURCO G, GENNARO R,
PAOLETTI S. (2009). Non-cytotoxic Silver Nanoparticle-Polysaccharide Nanocomposites with Antimicrobial Activity.
190
BIOMACROMOLECULES, vol. 10(6); p. 1429-1435, ISSN: 1525-7797, doi: 10.1021/bm900039x
• NYVALL COLLÉN P, LEMOINE M, DANIELLOU R, GUÉGAN JP, PAOLETTI S. (2009). Enzymatic degradation of κcarrageenan in aqueous solution. BIOMACROMOLECULES, vol. 10; p. 1757-1767, ISSN: 1525-7797, doi:
10.1021/bm9001766
• DANUSSI C, SPESSOTTO P, MUCIGNAT M.T, COSLOVI A, CAMPA C, UGGERI F, PAOLETTI S., COLOMBATTI A
(2009). A newly generated functional antibody identifies Tn antigen as a novel determinant in cancer cell-lymphatic
endothelium interaction. GLYCOBIOLOGY, vol. 19; p. 1056-1067, ISSN: 0959-6658, doi: 10.1093/glycob/cwp085
• DONATI I, ASARO F, PAOLETTI S. (2009). Experimental evidences of counterion affinity in alginates: the case of
nongelling ion Mg2+. JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES,
INTERFACES & BIOPHYSICAL, vol. 113; p. 12877-12886, ISSN: 1520-6106, doi: 10.1021/JP902912M
• DONATI I, MØRCH I, STRAND B, PAOLETTI S., SKJAAK-BRAEK G (2009). Effect of elongation of alternating
sequences on swelling behavior and large deformation properties of natural alginate gels. JOURNAL OF PHYSICAL
CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL, vol. 113; p. 1291612922, ISSN: 1520-6106, doi: 10.1021/jp905488u
23. PASQUATO Lucia
• GUARISE C, PASQUATO L., DE FILIPPIS V, SCRIMIN P (2006). Gold nanoparticles-based protease assay.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 103; p.
3978-3982, ISSN: 0027-8424
• PASQUATO L. (2006). La reazione di metatesi: una reazione da Nobel. AIM MAGAZINE, vol. 61; p. 48-54, ISSN:
1974-9635
• BELLANDA M, MAMMI S, GEREMIA S, DEMITRI N, RANDACCIO L, BROXTERMAN Q. B, KAPTEIN B, PENGO P,
PASQUATO L., SCRIMIN P (2007). Solvent polarity controls the Helical Conformation of Short Peptides Rich in Cαtetrasubstituted amino acids. CHEMISTRY-A EUROPEAN JOURNAL, vol. 13; p. 407-416, ISSN: 0947-6539
• PENGO P, BALTZER L, PASQUATO L., P. SCRIMIN (2007). Substrate modulation of the activity of an artificial
nanoesterase made of peptide-funtionalized gold nanoparticles. ANGEWANDTE CHEMIE. INTERNATIONAL EDITION,
vol. 45; p. 400-404, ISSN: 1433-7851
• HICKEY N, LAROCHETTE P. A, GENTILINI C, SORDELLI L, OLIVI L, POLIZZI S, MONTINI T, FORNASIERO P,
PASQUATO L., GRAZIANI M (2007). Monolayer protected gold nanoparticles on ceria for an efficient CO oxidation
catalyst. CHEMISTRY OF MATERIALS, vol. 19; p. 650-651, ISSN: 0897-4756
• GEORGAKILAS V, GOURNIS D, TZITZIOS V, PASQUATO L., GULDI D. M, PRATO M (2007). Decorating Carbon
Nanotubes with Metal or Semiconductor Nanoparticles. JOURNAL OF MATERIALS CHEMISTRY, vol. 17; p. 26792694, ISSN: 0959-9428
• MARTIN M, MANEA F, FIAMMENGO, R, PRINS L. J, PASQUATO L., SCRIMIN P (2007). Metallodendrimers as
transphosphorilation catalysts. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 129; p. 6982-6983, ISSN:
0002-7863
• GUARISE C, MANEA F, ZAUPA G, PASQUATO L., PRINS L. J, SCRIMIN P (2008). Cooperative Nanosystems.
JOURNAL OF PEPTIDE SCIENCE, vol. 14; p. 174-183, ISSN: 1075-2617, doi: 10.1002/psc.952
• MANEA F, PASQUATO L., PRINS L. J, SCRIMIN P (2007). Multivalent catalysts for the cleavage of nucleic acids
and their models. NUCLEIC ACIDS SYMPOSIUM SERIES, vol. 51; p. 67-68, ISSN: 0261-3166
• ZAMBON E, GIVANETTI, R, COTARCA L, PASQUATO L. (2008). Mechanistic investigation on 2-azaspiro[4,5]decan-3-one formation from 1-(aminomethyl)cyclohexylacetic acid (gabapentin). TETRAHEDRON, vol.
64; p. 6739-6743, ISSN: 0040-4020, doi: 10.1016/j.tet.2008.05.010
• GENTILINI C, BOCCALON M, PASQUATO L. (2008). Straightforward synthesis of fluorinated amphiphilic thiols.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY; p. 3308-3313, ISSN: 1434-193X, doi: 10.1002/ejoc.200800113
• GENTILINI C, EVANGELISTA F, RUDOLF P, FRANCHI P, LUCARINI M, PASQUATO L. (2008). Water-soluble gold
nanoparticles protected by fluorinated amphiphilic thiolates. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol.
130; p. 15678-15682, ISSN: 0002-7863, doi: 10.1021/ja8058364
• C. GENTILINI, P. FRANCHI, E. MILEO, S. POLIZZI, M. LUCARINI, PASQUATO L. (2009). Formation of patches in
3D monolayers driven by thiols with immiscible chains revealed by ESR spectroscopy. ANGEWANDTE CHEMIE.
INTERNATIONAL EDITION, vol. 47; p. 3060-3064, ISSN: 1433-7851, doi: 10.1002/anie.200805321
• GENTILINI C, PASQUATO L. (2010). Morphology of Mixed-Monolayers Protecting Metal Nanoparticles. JOURNAL
OF MATERIALS CHEMISTRY, vol. 20; p. 1403-1412, ISSN: 0959-9428, doi: 10.1039/b912759c
• LUCARINI M., PASQUATO L. (2010). ESR spectroscopy as a tool to investigate the properties of self-assembled
monolayers protecting metal nanoparticles. NANOSCALE, vol. 2; p. 668-676, ISSN: 2040-3364, doi:
10.1039/b9nr00384c
• CARGNELLO M., GENTILINI C., MONTINI T., FONDA E., MEHRAEEN S., CHI M., HERRERA-COLLADO M.,
BROWNING N.D., POLIZZI S., PASQUATO L., FORNASIERO P. (2010). Active and Stable Embedded Au@CeO2
Catalysts for Preferential Oxidation of CO. CHEMISTRY OF MATERIALS, vol. 22; p. 4335-4345, ISSN: 0897-4756,
doi: 10.1021/cm101499x
• PENGO P., PASQUATO L. (2010). Modified gold nanoparticles and surfaces. In: Rurack K., Martinez-Manez R..
Supramolecular chemistry of organic- inorganic-hybrid material. p. 113-154, New York: John Wiley & Sons,
ISBN/ISSN: 9780470376218
24. PRICL Sabrina
• FERMEGLIA M, COSOLI P, FERRONE M., PICCAROLO S, MENSITIERI G, PRICL S. (2006). PET/PEN Blends of
Industrial Interest as Barrier Materials. Part I. Many-Scale Molecular Modeling of PET/PEN Blends. POLYMER, vol.
47; p. 5979-5989, ISSN: 0032-3861
• TOTH R, FERRONE M., MIERTUS S, CHIELLINI E, FERMEGLIA M, PRICL S. (2006). Structure and energetics of
biocompatible polymer nanocomposite sistems: a molecular dynamics study. BIOMACROMOLECULES, vol. 7; p.
1714-1719, ISSN: 1525-7797
• TAMBORINI E, PRICL S., NEGRI T, LAGONIGRO M.S, MISELLI F, GRECO A, GRONCHI A, CASALI P, FERRONE M,
FERMEGLIA M, PIEROTTI M.A, PILOTTI S (2006). Functional analyses and Molecular modeling of two c-Kit
mutations responsible for Imatinib secondary resistance in GIST patients. ONCOGENE, vol. 25; p. 6140-6146,
191
ISSN: 0950-9232
• ZAMPIERI D, MAMOLO M.G, VIO L, BANFI E, SCIALINO G, FERMEGLIA M, FERRONE M, PRICL S. (2007).
Synthesis, antifungal and antimycobacterial activities of new bis-imidazole derivatives, and prediction of their
binding to P45014DM by molecular docking and MM/PBSA method. BIOORGANIC & MEDICINAL CHEMISTRY, vol.
15; p. 7444-7458, ISSN: 0968-0896
• SCOCCHI G, POSOCCO P, PRICL S., FERMEGLIA M. (2007). To the nanoscale and beyond! Multiscale molecular
modelling of polymer-clay nanocomposites. FLUID PHASE EQUILIBRIA, vol. 261; p. 366-374, ISSN: 0378-3812
• POSOCCO P, FERRONE M, FERMEGLIA M, PRICL S. (2007). Binding at the core. Computational study of structural
and ligand binding properties of naphthiridine based dendrimers. MACROMOLECULES, vol. 40; p. 2257-2266,
ISSN: 0024-9297
• SCOCCHI G, POSOCCO P, FERMEGLIA M, PRICL S. (2007). Polymer-Clay Nanocomposites: A Multiscale Molecular
Modeling Approach. JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES,
INTERFACES & BIOPHYSICAL, vol. 111; p. 2143-2151, ISSN: 1520-6106
• COSOLI P, SCOCCHI G, PRICL S., FERMEGLIA M (2008). Many-scale molecular simulation for ABS–MMT
nanocomposites: Upgrading of industrial scraps. MICROPOROUS AND MESOPOROUS MATERIALS, vol. 107; p. 169179, ISSN: 1387-1811
• FERMEGLIA M., PRICL S. (2007). Multiscale modeling for polymer systems of industrial interest. PROGRESS IN
ORGANIC COATINGS, vol. 58; p. 18-199, ISSN: 0300-9440
• CARTA A, LORIGA G, PIRASA S, PAGLIETTI G, LA COLLA P, COLLU G, PIANO M.A, LODDO R, FERRONE M,
FERMEGLIA M, PRICL S. (2006). Synthesis and in vitro evaluation of the anti-viral activity of N-[4-(1H(2H)benzotriazol-1(2)-yl)phenyl]alkylcarboxamides. MEDICINAL CHEMISTRY, vol. 2; p. 577-589, ISSN: 1573-4064
• PRICL S., TAMBORINI E, PIEROTTI M, PILOTTI S (2006). Response of a KIT-Positive Extra-abdominal
Fibromatosis to Imatinib Mesylate and KIT Genetic Analysis. JOURNAL OF THE NATIONAL CANCER INSTITUTE, vol.
98; p. 1583-1584, ISSN: 0027-8874
• CARTA A, LORIGA M, PAGLIETTI G, FERRONE M, FERMEGLIA M, PRICL S., SANNA T, IBBA C, LA COLLA P,
LODDO R (2007). Design, synthesis and preliminary in vitro and in silico antiviral activity of [4,7]phenantrolines
and 1-oxo-1,4-dihydro-[4,7]phenantrolines against a single-stranded positive sense RNA genome viruses.
• FERMEGLIA M, BRAIUCA P, GARDOSSI L., PRICL S., HALLING PJ (2006). In silico prediction of medium effects on
esterification equilibrium using the COSMO-RS method. BIOTECHNOLOGY PROGRESS, vol. 22; p. 1146-1152,
ISSN: 8756-7938
• MAZZEI M, NIEDDU E, MIELEA M, BALBIA, A, FERRONE M, FERMEGLIA M, MAZZEI M.T, PRICL S., LACOLLA P,
MARONGIU F, IBBA C, LODDO R (2008). Activity of Mannich bases of 7-hydroxycoumarin against Flaviviridae.
• MENSITIERI G, LAROBINA D, GUERRA G, VENDITTO V, FERMEGLIA M., PRICL S. (2008). Chloroform sorption in
nanoporous crystalline and amorphous phases of syndiotactic polystyrene. JOURNAL OF POLYMER SCIENCE. PART
B, POLYMER PHYSICS, vol. 46; p. 8-15, ISSN: 0887-6266
• CARTA A, LORIGA M, PIRAS S, PAGLIETTI G, FERRONE M, FERMEGLIA M, PRICL S., COLLU G, SANNA T, LODDO
R (2007). SYNTHESIS AND ANTI-PICORNAVIRIDAE IN VITRO ACTIVITY OF A NEW CLASS OF HELICASE
INHIBITORS THE N,N’-BIS-[4-1H(2H)-BENZOTRIAZOL-1(2)-YL)PHENYL] ALKYLDICARBOXAMIDES. MEDICINAL
• FERRONE M., PERRONE F, TAMBORINI E, PANENI M.S, FERMEGLIA M, SUARDI S, PASTORE E, DELIA D,
PIEROTTI M.A, PRICL S., PILOTTI S (2006). Functional analysis and molecular modelling show a preserved wild
type activity of p53 C238Y. MOLECULAR CANCER THERAPEUTICS, vol. 5; p. 1467-1473, ISSN: 1535-7163
• PRICL S., FERRONE M, FERMEGLIA M, AMATO F, COSENTINO C, CHENG M.M.C, WALCZAK R, FERRARI M (2006).
Multiscale modeling of protein transport in silicon membrane nanochannels. Part 1. Derivation of molecular
parameters from computer simulations. BIOMEDICAL MICRODEVICES, vol. 8; p. 277-290, ISSN: 1387-2176
• PRICL S., FERRONE M, FERMEGLIA M., AMATO F, COSENTINO C, CHENG M.M.C, WALCZAK R, FERRARI M
(2006). Multiscale modeling of protein transport in silicon membrane nanochannels. Part 1. Derivation of molecular
parameters from computer simulations. BIOMEDICAL MICRODEVICES, vol. 8(4); p. 277-290, ISSN: 1387-2176,
doi: 10.1007/S10544-006-0031-2
• COSOLI P, FERRONE M, PRICL S., FERMEGLIA M. (2007). Grand canonical Monte Carlo simulations for VOCs
adsorption in non-polar zeolites. INTERNATIONAL JOURNAL OF ENVIRONMENTAL TECHNOLOGY AND
MANAGEMENT, vol. 7; p. 228-243, ISSN: 1466-2132
• COSOLI P, FERRONE M, PRICL S., FERMEGLIA M (2008). Hydrogen sulphide removal from biogas by zeolite
adsorption-part II: a MD simulation. CHEMICAL ENGINEERING JOURNAL, vol. 145; p. 93-99, ISSN: 1385-8947
• COSOLI P, FERRONE M, PRICL S., FERMEGLIA M (2008). Hydrogen sulphide removal from biogas by zeolite
adsorption: Part I. GCMC molecular simulations. CHEMICAL ENGINEERING JOURNAL, vol. 145; p. 86-92, ISSN:
1385-8947
• MCAULIFFE J.C, WANG W.-L, PAVAN G.M, PRICL S., YANG D, CHEN S, LAZAR A.J.F, POLLOCK R.E, TRENT J.C
(2008). Unlucky number 13? Differential effects of KIT exon 13 mutation in gastrointestinal stromal tumors.
MOLECULAR ONCOLOGY, vol. 2; p. 161-163, ISSN: 1574-7891
• MICHELE TONELLI, VITO BOIDO, CATERINA CANU, SPARATORE A, SPARATORE F, PANENI M.S, FERMEGLIA M,
PRICL S., LA COLLA P, CASULA L, IBBA C, COLLU D, LODDO R (2008). Antimicrobial and cytotoxic
arylazoenamines. Part III: Antiviral activity of selected classes of arylazoenamines. BIOORGANIC & MEDICINAL
• MALY M, POSOCCO P, PRICL S., FERMEGLIA M. (2008). Self-Assembly of Nanoparticle Mixtures in Diblock
Copolymers: Multiscale Molecular Modeling. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, vol. 47; p.
5023-5038, ISSN: 0888-5885
• MALY M, POSOCCO P, FERMEGLIA M, PRICL S. (2008). Scripting approach in hybrid organic-inorganic
condensation simulation: the GPTMS proof-of-concept. MOLECULAR SIMULATION, vol. 34; p. 1215-1236, ISSN:
0892-7022
• NEGRI T, PAVAN G.M, VIRDIS E, GRECO A, FERMEGLIA M., PRICL S., PIEROTTI M.A, PILOTTI S, TAMBORINI E
192
(2009). T670X KIT mutations in gastrointestinal stromal tumors: making sense of missense. JOURNAL OF THE
NATIONAL CANCER INSTITUTE, vol. 101; p. 194-204, ISSN: 0027-8874
• CARTA A, PIRAS, S, PAGLIETTI G, PRICL S., LA COLLA P, BUSONERA B, LODDO R (2008). 2(3)-aryl-thio(oxy)methylquinoxaline derivatives: a new class of P-glycoprotein-mediated drug efflux inhibitor. MEDICINAL
• PRICL S. (2008). Quo vadis affinity? Clinical evidences
and computer-assisted simulations in the
Imatinib saga. EUROPEAN JOURNAL OF NANOMEDICINE, vol. 2; p. 21-31, ISSN: 1662-5986
• TONELLI M, VAZZANA I, TASSO B, BOIDO V, SPARATORE F, FERMEGLIA M, PANENI M.S, POSOCCO P, PRICL S.,
LA COLLA P, IBBA C, SECCI B, COLLU G, LODDO R (2009). Antiviral and cytotoxic activities of aminoarylazo
compounds
and aryltriazene derivatives. BIOORGANIC & MEDICINAL CHEMISTRY, vol. 17; p. 4425-4440, ISSN: 0968-0896
• FERMEGLIA M, PRICL S. (in stampa). Multiscale molecular modeling in nanostructured material design and
process
system engineering. COMPUTERS & CHEMICAL ENGINEERING, ISSN: 0098-1354, doi:
10.1016/j.compchemeng.2009.04.006
• ZAMPIERI D, MAMOLO MG, LAURINI E, FERMEGLIA M, POSOCCO P, PRICL S., BANFI E, SCIALINO G, VIO L
(2009). Antimycobacterial activity of new 3,5-disubstituted 1,3,4-oxadiazol-2(3H)-one derivatives. Molecular
modeling investigations. BIOORGANIC & MEDICINAL CHEMISTRY, vol. 17; p. 4693-4707, ISSN: 0968-0896
• ZAMPIERI D., M.G. MAMOLO, E. LAURINI, C. FLORIO, C. ZANETTE, M. FERMEGLIA, P. POSOCCO, M.S. PANENI,
PRICL S., L. VIO (2009). Synthesis, biological evaluation, and three-dimensional in silico pharmacophore model for
sigma1 receptor ligand based on a series of benzo[d]oxazol-2(3H)-one derivatives. JOURNAL OF MEDICINAL
• PAVAN G.M, DANANI A, PRICL S., SMITH, D.K (2009). Modeling the multivalent recognition between dendritic
molecules and DNA: Understanding how ligand "sacrifice" and screening can enhance binding. JOURNAL OF THE
AMERICAN CHEMICAL SOCIETY, vol. 131; p. 9686-9694, ISSN: 0002-7863
• POSOCCO P, PAVAN G, SCOCCHI G, HANDGRAAF J.W, MALEK A, MALY M, FERMEGLIA M, FRAAIJE J, CATAPANO
V, DANANI A, PRICL S. (2009). Base Invaders. Coupling Experiments and Multiscale Modeling of Dendrimer-Based
siRNA Delivery Agents. In: VINCENZINI P., DE ROSSI D.. Advances in Science and Technology: Smart Materials &
Micro/Nanosystems. vol. 57, p. 154-159, BASEL: Trans Tech Publications, ISBN/ISSN: 3-908158-20-6
• ANGUSTI A, MANFREDINI S, DURINI E, CILIBERTI N, VERTUANI S, SOLAROLI N, PRICL S., FERRONE M,
FERMEGLIA M., LODDO R, SECCI B, VISIOLI A, SANNA T, COLLU G, PEZZULLO M, LA COLLA P (2008). Design,
synthesis and anti flaviviridae activity of N6-, -O- and 5 O-substituted adenine nucleoside analogs. CHEMICAL &
PHARMACEUTICAL BULLETIN, vol. 56; p. 423-432, ISSN: 0009-2363
• SCOCCHI G, POSOCCO P, HANDGRAAF J.W, FRAAIJE J.G.E.M, FERMEGLIA M., PRICL S., S (2009). A complete
multiscale modelling approach for polymer-clay nanocomposites. CHEMISTRY-A EUROPEAN JOURNAL, vol. 15; p.
7586-7592, ISSN: 0947-6539
• FERMEGLIA M., PRICL S. (2009). Multiscale molecular modeling in nanostructured material design and process
system engineering. COMPUTERS & CHEMICAL ENGINEERING, vol. 33; p. 1701-1710, ISSN: 0098-1354
• FERMEGLIA M, MALY M, POSOCCO P, PRICL S. (2009). Multiscale Molecular Modeling of Hybrid Organic-Inorganic
Nanocomposites of Type I and II. In: VINCENZINI P., D'ARRIGO, G.. Advances in Science and Technology: Smart
Materials & Micro/Nanosystems. vol. 54, p. 265-269, BASEL: Trans Tech Publications, ISBN/ISSN: 3-908158-20-6
• E. LAURINI, D. ZAMPIERI, M.G. MAMOLO, L. VIO, C. ZANETTE, C. FLORIO, P. POSOCCO, M. FERMEGLIA, PRICL
S. (2010). A 3D-Pharmacophore Model for σ2 Receptors Based on a Series of Substituted Benzo[d]oxazol-2(3H)one Derivatives. BIOORGANIC AND MEDICINAL CHEMISTRY LETTERS; p. 2954-2957, ISSN: 1464-3405
• JONES S.P., PAVAN G.M., DANANI A., PRICL S., SMITH D.K. (2010). Quantifying the effect of surface ligands on
dendron-DNA interactions: Insights into multivalency through a combined experimental and theoretical approach.
CHEMISTRY-A EUROPEAN JOURNAL, vol. 16; p. 4519-4532, ISSN: 0947-6539
• P. POSOCCO, Z. POSEL, M. FERMEGLIA, M. LISAL, PRICL S. (2010). A molecular simulation approach to the
prediction of the morphology of self-assembled nanoparticles in diblock copolymers. JOURNAL OF MATERIALS
CHEMISTRY, vol. 20; p. 10511-10520, ISSN: 0959-9428, doi: 10.1039/c0jm01561j
• PIEROTTI M, TAMBORINI E., NEGRI T., PRICL S., PILOTTI S. (2011). Targeted therapy in GIST: in silico
modeling for prediction of resistance. NATURE REVIEWS. CLINICAL ONCOLOGY, vol. 8; p. 161-170, ISSN: 17594774
• DILEO P, PRICL S., TAMBORINI E, NEGRI T, STACCHIOTTI S, GRONCHI A, POSOCCO P, LAURINI E, COCO P,
FUMAGALLI E, CASALI PG, PILOTTI S. (2011). Imatinib response in two GIST patients carrying two hitherto
functionally uncharacterized PDGFRA mutations: an imaging, biochemical and molecular modeling study.
INTERNATIONAL JOURNAL OF CANCER, vol. 128; p. 983-990, ISSN: 0020-7136
• TONELLI M, SIMONE M, TASSO B, NOVELLI F, BOIDO V, SPARATORE F, PAGLIETTI G, PRICL S., GILIBERTI G,
BLOIS S, IBBA C, SANNA G, LODDO R, LA COLLA P. (2010). Antiviral activity of benzimidazole derivatives. II.
Antiviral activity of 2-phenylbenzimidazole derivatives. BIOORGANIC & MEDICINAL CHEMISTRY, vol. 18; p. 29372953, ISSN: 0968-0896
• POSOCCO P, PRICL S., JONES ., BARNARD A, SMITH DK (2010). Less is more: multiscale modelling of selfassembling multivalency and its impact on DNA binding and gene delivery. CHEMICAL SCIENCE, vol. 1; p. 393404, ISSN: 2041-6520
• PIEROTTI MA, NEGRI T, TAMBORINI E, PERRONE F, PRICL S., PILOTTI S. (2010). Targeted therapies: the rare
cancer paradigm. MOLECULAR ONCOLOGY, vol. 4; p. 19-37, ISSN: 1574-7891
• POSOCCO P, FERMEGLIA M, PRICL S. (2010). Morphology prediction of block copolymers for drug delivery by
mesoscale simulations. JOURNAL OF MATERIALS CHEMISTRY, vol. 20; p. 7742-7753, ISSN: 0959-9428
• PRICL S. (2009). Quo vadis, affinity? Clinical evidences and computer-assisted
simulations in the Imatinib saga. EUROPEAN JOURNAL OF NANOMEDICINE, vol. 2; p. 22-30, ISSN: 1662-5986
25. SBAIZERO Orfeo
193
• SCUOR N., GALLINA P, SBAIZERO O., PANCHAWAGH H.V, MAHAJAN R.L (2006). Dynamic characterization of
MEMS cantilevers in liquid environment using a low-cost optical system. MEASUREMENT SCIENCE & TECHNOLOGY,
vol. 17; p. 173-180, ISSN: 0957-0233
• SBAIZERO O., SUN-YOUNG CHANG. SAOUMA V (2006). Numerical simulation of thermal residual stress in Moand FeAl-toughened alumina. COMPOSITES. PART B, ENGINEERING; p. 550-555, ISSN: 1359-8368
• SCUOR N., GALLINA, P, PANCHAWAGH H.V, MAHAJAN R.L, SBAIZERO O., SERGO V (2006). Design of a novel
MEMS platform for the biaxial stimulation of living cells. BIOMEDICAL MICRODEVICES, vol. 8; p. 239-246, ISSN:
1387-2176
• ZULIANI F, SBAIZERO O., CHOUDHURY R, DE VITA A (2007). Dipole formation due to ion exchange processes at
the metal-ceramic Al/MgAl2O4 (001) interface. ACTA MATERIALIA, vol. 55; p. 5813-5821, ISSN: 1359-6454
• MARRAS L, SBAIZERO O. (2009). The Fragmentation Test applied to adhesion measurements and
microstructural characterisation in plasma pretreated metallized plastic webs. PACKAGING TECHNOLOGY AND
SCIENCE, vol. 22; p. 293-302, ISSN: 0894-3214
• COMIN-CHIARAMONTI L, CAVALLERI G, SBAIZERO O., COMIN-CHIARAMONTI P (2009). Crystallochemical
comparison between Portland cements and Mineral Trioxide Aggregate (MTA). JOURNAL OF APPLIED
BIOMATERIALS & BIOMECHANICS, vol. 7(3); p. 171-178, ISSN: 1722-6899
• PISCANEC S, ZULIANI F, COLOMBI CIACCHI L, LEVITA G, SBAIZERO O., DEVITA A (2009). DFT modelling of
ceramic materials and interfaces. INTERNATIONAL JOURNAL OF MATERIALS & PRODUCT TECHNOLOGY, vol. 35(34); p. 271-292, ISSN: 0268-1900
26. SCHMID Chiara
• L. MOIMAS, M. BIASOTTO, R. DI LENARDA, A. OLIVO, SCHMID C. (2006). Rabbit pilot study on the resorbability
of three-dimensional bioactive glass fibre scaffolds. ACTA BIOMATERIALIA, vol. 2; p. 191-199, ISSN: 1742-7061
• M. LAZZARINO, E. DE MARCHI, M. BRESSANUTTI, L. VACCARI, S.CABRINI, SCHMID C., R. POETES AND G.
SCOLES (2006). Twin cantilevers with a nanogap for single molecule experimentation. MICROELECTRONIC
ENGINEERING, vol. 83; p. 1309-1311, ISSN: 0167-9317
• L. MOIMAS, G. DE ROSA, V. SERGO, SCHMID C. (2006). Bioactive porous scaffolds for tissue engineering
applications: investigation on the degradation process by Raman spectroscopy and scanning electron microscopy.
JOURNAL OF APPLIED BIOMATERIALS & BIOMECHANICS, vol. 4; p. 102-109, ISSN: 1722-6899
• M.BIASOTTO, F. ANTONIOLLI, N. SCUOR, V.RET, SCHMID C., R.DI LENARDA (2006). Verifica sperimentale della
adesione tra una resina composite e quattro materiali metallici. TEAMWORK, vol. VIII(4); p. 76-82, ISSN: 18252214
• L. MOIMAS, V. SERGO, SCHMID C., E.PIRHONEN (2006). Bioactive porous scaffolds for tissue engineering
applications: composition and immersion time effects on the degradation process. In: 19thESB. Sorrento, Italy, 1115 sept. 2005
• L.MOIMAS, M.BIASOTTO, R. DI LENARDA, A. OLIVO, SCHMID C. (2006). In situ Tissue Engineering, effect of a
porous bioactive glass scaffold on bone healing: in vivo rabbit study. In: Ceramics, cells and Tissues (Periodical
Conference series) Materials for scaffolding of biologically engineered systems Interfaces and interactions on a
nanoscale. Faenza - Italy, 23-27 maggio 2006, FAENZA: A. Ravaglioli, A. Krayewski ISTEC-CNR, p. 247-254,
ISBN/ISSN: 88-8080-071-X
• E. ANDREOLI, O. DELLA GASPERA, G.DE ROSA, L. MOIMAS, SCHMID C. (2006). Surface characterization of
bioglass fibers for biodegradabile polymer-ceramic composite scaffolds. JOURNAL OF APPLIED BIOMATERIALS &
BIOMECHANICS, vol. 4; p. 57, ISSN: 1722-6899
• O. DELLA GASPERA, G. DE ROSA, L. MOIMAS, SCHMID C. (2006). Sintering process of bioactive glass fibres into
a metallic mould: a feasibility study. JOURNAL OF APPLIED BIOMATERIALS & BIOMECHANICS, vol. 4; p. 64, ISSN:
1722-6899
• V. RET, M. BIASOTTO, F. ANTONIOLLI, C. SALVI, SCHMID C., E. DE STEFANO DORIGO (2006). Effect of alloy
finishing on metal-ceramic bonding: profilometric and SEM analysis. In: congresso internazionale dell'Aiop
(Accademia italiana di odontoiatria protesica). Bologna 23-25 novembre 2006, 23-25 novembre 2006
• A. PORTA, L. MOIMAS, V. SERGO, SCHMID C. (2006). Devitrification phenomena of large working range
bioglasses. In: 8° convegno nazionale AIMAT. Palermo, Italia, Palermo (I), 27 giugno-1 luglio 2006
• L. COREN, L. MOIMAS, SCHMID C. (2007). Vetri bioattivi: devetrificazione e comportamento in vitro. In:
congresso nazionale SIB. Bologna, Bologna 28-29 maggio 2007
• S. MAGGIOLINO, SCHMID C. (2008). Corrosion resistance in FSW and in MIG welding techniques of AA6XXX.
JOURNAL OF MATERIALS PROCESSING TECHNOLOGY, vol. 197; p. 237-240, ISSN: 0924-0136, doi:
10.1016/j.jmatprotec.2007.06.034
• L. MARSICH, L. MOIMAS, SERGO V., SCHMID C. (2009). Raman spectroscopic study of bioactive silica based
glasses: The role of the alkali/alkali earth ratio on the non-bridging oxygen/bridging oxygen (NBO/BO) ratio.
SPECTROSCOPY, vol. 23; p. 227-232, ISSN: 0712-4813, doi: 10.3233/SPE-2009-0380
• L. COZZARINI, S. MAGGIOLINO, SCHMID C. (2009). Comportamento a corrosione in ambiente marino di AA
6082-T6: confronto tra saldatura MIG e FSW. In: Atti “Giornate Nazionali sulla Corrosione e Protezione”, Udine 2425-26 giugno 2009. Udine, 24-25-26 giugno 2009, MILANO: Associazione Italiana di Metallurgia, p. 1-12,
ISBN/ISSN: ISBN 88-85298-70-2
• HAIMI S, GORIANC G, MOIMAS L, LINDROOS B, HUHTALA H, RÄTY S, KUOKKANEN H, SÁNDOR GK, SCHMID C.,
MIETTINEN S, SUURONEN R (2009). Characterization of zinc-releasing three-dimensional bioactive glass scaffolds
and their effect on human adipose stem cell proliferation and osteogenic differentiation. ACTA BIOMATERIALIA, vol.
5; p. 3122-3131, ISSN: 1742-7061
27. SERGO Valter
• L. MOIMAS, G. DE ROSA, SERGO V., SCHMID C. (2006). Bioactive porous scaffolds for tissue engineering
applications: investigation on the degradation process by Raman spectroscopy and scanning electron microscopy.
JOURNAL OF APPLIED BIOMATERIALS & BIOMECHANICS, vol. 4; p. 102-109, ISSN: 1722-6899
• KRAFFT C, SERGO V. (2006). Biomedical applications of Raman and
infrared spectroscopy to diagnose tissues. SPECTROSCOPY, vol. 20; p. 195-218, ISSN: 0887-6703
194
• DE MARIA L, RINALDI C, LUPETIN P, SERGO V. (2006). Portable optical system for in-situ thermal barrier
assessment of service operated blades. In: Proceedings of the ASME Turbo Expo 2006. Barcellona, Spain, May, 611, 2006, NEW YORK: American Society Mechanical Engineers, vol. 2, p. 647-654
• L.DE MARIA, C.RINALDI, A.MARTINELLI, SERGO V. (2006). “Non-destructive Integrity Assessment studies of
TBC coatings”. In: Proceedings of TURBINE FORUM 2006. Nizza, France, April, 26-28, 2006, NIZZA: Turbine
Forum, vol. 1
• TITILAYO A.K, UMERI A, SCUOR N, SERGO V. (2008). Raman investigation of the Ageing of Ni-BaTiO3 Multilayer
Ceramic Capacitors. JOURNAL OF MATERIALS SCIENCE, vol. 43; p. 922-926, ISSN: 0022-2461, doi:
10.1007/s10853-007-2215-4
• KRAFFT C, CODRICH D, PELIZZO G, SERGO V. (2008). Raman and FTIR imaging of lung tissue: methodology for
control
samples. VIBRATIONAL SPECTROSCOPY, vol. 46; p. 141-149, ISSN: 0924-2031, doi:
10.1016/j.vibspec.2007.12.007
• KRAFFT C, CODRICH D, PELIZZO G, SERGO V. (2008). Raman and FTIR Imaging of lung tissue: congenital cystic
adenomatoid malformation. ANALYST, vol. 133; p. 361-371, ISSN: 0003-2654, doi: 10.1039/B712958K
• MASCHIO S, ANEGGI E, TROVARELLI A, SERGO V. (2008). Influence of erbia or europia doping on crystal
structure and microsctructure of ceria-zirconia (CZ) solid solutions. CERAMICS INTERNATIONAL, vol. 34; p. 13271333, ISSN: 0272-8842, doi: 10.1016/j.ceramint.2007.03.006
• KRAFFT C, CODRICH D, PELIZZO G, SERGO V. (2008). Raman and FTIR microscopic imaging of colon tissue: a
comparative study. JOURNAL OF BIOPHOTONICS, vol. 1; p. 154-169, ISSN: 1864-063X, doi:
10.1002/jbio.200710005
• CODAN B, VISINTIN S, DE MARIA L, SERGO V. (2006). A study on the effect of micro and nano-debris impact on
aerospace thermal barrier coating at high temperature. In: Proceedings AIAA 57th International Astronautical
Congress, IAC 2006. Valencia, Spain, October, 2-6 2006, NEW YORK: AIAA, vol. 8, p. 5652-5662, ISBN/ISSN:
978-160560039-0
• CODAN B, SERGO V. (2008). Implementation of maskless laser lithography using a Raman spectroscopy
microprobe. REVIEW OF SCIENTIFIC INSTRUMENTS, vol. 79, ISSN: 0034-6748, doi: 10.1063/1.2981701
• L. MARSICH, L. MOIMAS, SERGO V., C. SCHMID (2009). Raman spectroscopic study of bioactive silica based
glasses: The role of the alkali/alkali earth ratio on the non-bridging oxygen/bridging oxygen (NBO/BO) ratio.
SPECTROSCOPY, vol. 23; p. 227-232, ISSN: 0712-4813, doi: 10.3233/SPE-2009-0380
• BONIFACIO A., FINAURINI S, KRAFFT C, PARAPINI S, TARAMELLI D, SERGO V. (2008). Spatial distribution of
heme species in erythrocytes infected with Plasmodium falciparum by use of resonance Raman imaging and
multivariate analysis. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 392; p. 1277-1282, ISSN: 1618-2642,
doi: 10.1007/s00216-008-2414-0
• DONATI I., TRAVAN A, PELILLO C, SCARPA T, COSLOVI A, BONIFACIO A, SERGO V., PAOLETTI S (2009). Polyol
Synthesis of Silver Nanoparticles: Mechanism of Reduction by Alditol Bearing Polysaccharides.
BIOMACROMOLECULES, vol. 10(2); p. 210-213, ISSN: 1525-7797, doi: 10.1021/BM801253C
• B. CODAN, T. GAIOTTO, R. DI NIRO, R. MARZARI, SERGO V. (2009). Patterning of fibronectin using laser writer
for force measurement in cells. In: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VI,
71880P. San Jose (CA, U,S.A.), 24-29/01/09, SAN JOSE: Alexander N. Cartwright; Dan V. Nicolau, Editors,, vol.
71880P, p. 1178-1185, doi: 10.1117/12.809698
• B. CODAN, T. GAIOTTO, R. DI NIRO, R. MARZARI, SERGO V. (2008). Implementation of fibronectin patterning
with a Raman spectroscopy microprobe for focal adhesions studies in cells. In: Proceedings of International
Congress of Biomedica Engineering 2008. Singapore, 03/12/2008
• KRAFFT C, CODRICH D, PELIZZO G, SERGO V. (2009). Raman and FTIR imaging of lung tissue:
bronchopulmonary sequestration. JOURNAL OF RAMAN SPECTROSCOPY, vol. 40; p. 595-603, ISSN: 0377-0486
• NAVARRA C.O, CADENARO M, ARMSTRONG S.R, JESSOP J, ANTONIOLLI F, SERGO V., DI LENARDA R, BRESCHI
10.1016/J.DENTAL.2009.05.009
• NAVARRA C.O, CADENARO M, CODAN B, MAZZONI A, SERGO V., DE STEFANO DORIGO E, BRESCHI L (2009).
• AFFATATO S, TRAINA F, MAZZEGA-FABBRO C, SERGO V., VICECONTI, M (2009). Is ceramic-on-ceramic
squeaking phenomenon reproducible in vitro? A long-term simulator study under severe conditions. JOURNAL OF
BIOMEDICAL MATERIALS RESEARCH. PART B, APPLIED BIOMATERIALS., vol. 91; p. 264-271, ISSN: 1552-4973,
doi: 10.1002/jbm.b.31398
• MARCHESI G., NAVARRA C.O., CADENARO M., CARRILHO M.R., CODAN B., SERGO V., DI LENARDA R., BRESCHI
L. (2010). The effect of ageing on the elastic modulus and degree of conversion of two multistep adhesive systems.
• BONIFACIO A., SERGO V. (2010). Effects of sample orientation in Raman microspectroscopy of collagen fibers
and their impact on the interpretation of the amide III band. VIBRATIONAL SPECTROSCOPY, vol. 53; p. 314-317,
ISSN: 0924-2031, doi: doi:10.1016/j.vibspec.2010.04.004
• LUGHI V., SERGO V. (2010). Low temperature degradation -aging- of zirconia: a critical review of the relevant
aspects in dentistry. DENTAL MATERIALS, vol. 26; p. 807-820, ISSN: 0109-5641, doi:
10.1016/j.dental.2010.04.006
• MILLO D., BONIFACIO A., MONCELLI M.R., SERGO V., GOOIJER C., VAN DER ZWAN G. (2010). Characterization
of hybrid bilayer membranes on silver electrodes as biocompatible SERS substrates to study membrane-protein
interactions. COLLOIDS AND SURFACES. B, BIOINTERFACES, vol. 81 (1); p. 212-216, ISSN: 0927-7765
• BONIFACIO A., BELEITES C., VITTUR F., MARSICH E., SEMERARO S., PAOLETTI S., SERGO V. (2010). Chemical
imaging of articular cartilage sections with Raman mapping, employing uni- and multi-variate methods for data
analysis. ANALYST, vol. 135; p. 3193-3294, ISSN: 0003-2654
195
28. STANTA Giorgio
• STANTA G., POZZI MUCELLI S, PETRERA F, BONIN S, BUSSOLATI G (2006). A Novel Fixative Improves
Opportunities of Nucleic Acids and Proteomic Analysis in Human Archive's Tissues. DIAGNOSTIC MOLECULAR
PATHOLOGY, vol. 15(2); p. 115-123, ISSN: 1052-9551
• TOTHOVA SM, BONIN S, TREVISAN G., STANTA G. (2006). Mycosis fungoides: is it a Borrelia burgdorferiassociated disease?. BRITISH JOURNAL OF CANCER, vol. 94; p. 879-883, ISSN: 0007-0920
• POZZI MUCELLI S., ODREMAN F., GERARDI E., STANTA G., VINDIGNI A. (2006). Proteomic studies on the white
matter of human brain. JOURNAL OF CHROMATOGRAPHY. B, vol. 833(1); p. 80-90, ISSN: 1570-0232
• GARDIOL D, ZACCHI A, PETRERA F, STANTA G., BANKS L (2006). Human disc large and scrib are localized at the
same regions in colon mucosa and changes in their expression patterns are correlated with loss of tissues
architecture during malignant progression. INTERNATIONAL JOURNAL OF CANCER, vol. 119; p. 1285-1290, ISSN:
0020-7136
• SCAGGIANTE B., BONIN S, CRISTIANO L, SIRACUSANO S, STANTA G., DAPAS B, GIANSANTE C, FIOTTI N,
GRASSI G (2008). Prostate Tumour-Inducing gene-1 analysis in human prostate cancer cells and tissue in relation
to Mycoplasma infection. CANCER INVESTIGATION, vol. 26(8); p. 800-808, ISSN: 0735-7907
• BONIN S, BRUNETTI D, BENEDETTI E, DOTTI I, GORJI N, STANTA G. (2008). MOLECULAR CHARACTERISATION
OF BREAST CANCER PATIENTS AT HIGH AND LOW RECURRENCE RISK. VIRCHOWS ARCHIV, vol. 452; p. 241-250,
ISSN: 0945-6317
• STANTA G., CESCATO A, BONIN S., BARBAZZA R (2008). BIOETHICS CONSIDERATIONS FOR MEDICAL
RESEARCH IN HUMAN ARCHIVE TISSUES: THE POINT OF VIEW OF THE RESEARCHER. VIRCHOWS ARCHIV, vol.
453; p. 117-119, ISSN: 0945-6317
• MICHELUCCI A, DI CRISTOFANO C, LAMI A, COLLECCHI P, CALIGO A, DECARLI N, LEOPIZZI M, ARETINI P,
BERTACCA G, PROSPERI PORTA R, RICCI S, DELLA ROCCA C, STANTA G., BEVILACQUA G, CAVAZZANA A (2008).
PIK3CA IN BREAST CARCINOMA. A MUTATIONAL ANALYSIS OF SPORADIC AND HEREDITARY CASES. DIAGNOSTIC
MOLECULAR PATHOLOGY, ISSN: 1052-9551
• STANTA G., CESCATO A, BARBAZZA R (2008). BIOETHICAL CONSIDERATIONS ON MEDICAL RESEARCH USING
HUMAN TISSUES: THE RESEARCHER'S VIEWPOINT. PATHOLOGICA, vol. 100 (2); p. 67-75, ISSN: 0031-2983
• NARDON E, DONADA M, BONIN S., DOTTI I, STANTA G. (2009). Higher random oligo concentration improves
reverse transcription yield of cDNA from bioptic tissues and quantitative RT-PCR reliability. EXPERIMENTAL AND
MOLECULAR PATHOLOGY, vol. 87; p. 146-151, ISSN: 0014-4800, doi: 10.1016/j.yexmp.2009.07.005
• POZZI MUCELLI S, ZAMUNER M, TORMEN M, STANTA G., UGO P (2008). NANOELECTRODE ENSEMBLES AS
RECOGNITION PLATFORM FOR ELECTROCHEMICAL IMMUNOSENSORS. BIOSENSORS & BIOELECTRONICS, vol. 23
(12); p. 1900-1903, ISSN: 0956-5663
• BONIN S., BRUNETTI D, BENEDETTI E, DOTTI I, GORJI N, STANTA G. (2008). Molecular characterisation of
breast cancer patients at high and low recurrence risk. VIRCHOWS ARCHIV, vol. 452; p. 241-250, ISSN: 09456317, doi: 10.1007/s00428-007-0570-9
• DOTTI I, BONIN S, BASILI G, NARDON E, BALANI A, SIRACUSANO S, ZANCONATI F, PALMISANO S, DE MANZINI
N, STANTA G. (in stampa). EFFECTS OF FORMALIN, METHACARN AND FINEFIX FIXATIVES ON RNA
PRESERVATION. DIAGNOSTIC MOLECULAR PATHOLOGY; p. ---, ISSN: 1052-9551
29. UGO Paolo
• P.SCOPECE, L.M.MORETTO, S.POLIZZI, P.UGO (2006). Composite films of poly-(ester-sulphonated) and poly-(3methylthiophene) for ion-exchange voltammetry in acetonitrile solutions. ELECTROCHIMICA ACTA, vol. 51; p.
2153-2160, ISSN: 0013-4686
• P.SCOPECE, L.A.BAKER, P.UGO, C.R.MARTIN (2006). Conical nanopore membranes: solvent shaping of
nanopores. NANOTECHNOLOGY, vol. 17; p. 1-6, ISSN: 0957-4484, doi: 10.1088/0957-4484/17/15/057
• O.BURIEZ, L.M.MORETTO, P.UGO (2006). Ion-exchange volatmmetry of tris-(2,2'-bipyridine) nickel(II),
cobalt(II), and Co(salen)at polyestersulfonated ionomer coated electrodes in acetonitrile: Reactivity of the
electrogenerated low-valent complexes. ELECTROCHIMICA ACTA, vol. 52; p. 958-964, ISSN: 0013-4686, doi:
10.1016/j.electacta.2006.06.030
• PEREIRA F.C, BERGAMO E.P, BOLDRIN ZANONI M.V, MORETTO L.M., P.UGO (2006). Aplicações de nano
eletrodos como sensores na química analítica. QUIMICA NOVA, vol. 29; p. 1054-1060, ISSN: 0100-4042
• P.UGO (2006). Polymer based voltammetric sensors. In: C.A. GRIMES, E.C. DICKEY, M.V. PISHKO. Encyclopedia
of Sensors. vol. 8, p. 67-86, STEVENSON RANCH, CALIFORNIA: American Scientific Publisher, ISBN/ISSN: 158883-064-0
• F.C. PEREIRA, L.M. MORETTO, M. DE LEO, M.V. BODRIN ZANONI, P.UGO (2006). Gold nanoelectrode ensembles
for direct trace electroanalysis of iodide. ANALYTICA CHIMICA ACTA, vol. 575; p. 16-24, ISSN: 0003-2670
• M.DE LEO, A. KUHN, P.UGO (2007). 3D-Ensembles of gold nanowires: preparation, characterization and
electroanalytical prospects. ELECTROANALYSIS, vol. 19; p. 227-236, ISSN: 1040-0397, doi:
10.1002/elan.200603724
• P.UGO, L.M. MORETTO (2007). Template deposition of metals. In: C. ZOSKI. HANDBOOK OF
ELECTROCHEMISTRY. p. 678-709, AMSTERDAM: Elsevier
• DE LEO M, PEREIRA F.C, MORETTO L.M., SCOPECE P, POLIZZI S, P.UGO (2007). Towards a better understanding
of gold electroless deposition in track-etched templates. CHEMISTRY OF MATERIALS, vol. 19; p. 5955-5964, ISSN:
0897-4756
• PEREIRA F.C, BOLDRIN ZANONI M.V, MORETTO L.M., P.UGO (2007). OPTICAL AND MORPHOLOGICAL
CHARACTERISTICS OF GOLD NANOSTRUCTURES. QUIMICA NOVA, vol. 30; p. 1550-1554-, ISSN: 0100-4042
• S. POZZI MUCELLI, M. ZAMUNER, M. TORMEN, G. STANTA, P.UGO (2008). Nanoelectrode ensembles as
recognition platform for electrochemical immunosensors. BIOSENSORS & BIOELECTRONICS, vol. 23; p. 19001903, ISSN: 0956-5663, doi: 10.1016/j.bios.2008.02.027
• MORETTO L.M., KOHLS T, CHOVIN A, SOJIC N, P.UGO (2008). Epifluorescence imaging of electrochemically
Switchable Langmuir-Blodgett films of Nafion. LANGMUIR, vol. 24; p. 6367-6374, ISSN: 0743-7463
• L.M. MORETTO, S. PANERO, B. SCROSATI, P.UGO (2009). Templated ensembles of nanoelectrodes. In: YUEHE
196
LIN, HARI SINGH NALWA. Handbook of electrochemical nanotechnology. vol. 1, p. 87-105, STEVENSON RANCH:
American Scientific Publishers, ISBN/ISSN: 1-58883-121-3
• M. DE LEO, L. M. MORETTO, O. BURIEZ, P.UGO (2009). ELECTROCHEMICAL BEHAVIOR OF NANOELECTRODE
ENSEMBLES IN THE IONIC LIQUID [BMIM][BF4]. ELECTROANALYSIS, vol. 21; p. 392-398, ISSN: 1040-0397, doi:
10.1002/elan.200804414
• M.ZAMUNER, S. POZZI MUCELLI, M. TORMEN, G. STANTA, P.UGO (2008). Electrochemical nanobiosensors and
protein detection. EUROPEAN JOURNAL OF NANOMEDICINE, vol. 1; p. 33-36, ISSN: 1662-5986, doi:
10.3884/0001.8
• M.ZAMUNER, D. TALAGA, F. DEISS, V. GUIEU, A. KUHN, P.UGO, N. SOJIC (2009). Fabrication of a macroporous
microwell array for surface-enhanced raman scattering. ADVANCED FUNCTIONAL MATERIALS, vol. 19; p. 31293135, ISSN: 1616-301X, doi: 10.1002/adfm.200900752
• L.M. MORETTO, M. DE LEO, P.UGO (2009). Preliminary studies on the iodide determination in the marine
environment by nanoelectrode ensembles. BOLLETTINO DI GEOFISICA TEORICA E APPLICATA, vol. 50; p. 361368, ISSN: 0006-6729
• P.UGO, LIGIA M. MORETTO, MANUELA DE LEO, ANDREW P. DOHERTY, CHIARA VALLESE, SREEKANTH
PENTLAVALLI (2010). Diffusion regimes at nanoelectrode ensembles in different ionic liquids. ELECTROCHIMICA
ACTA, vol. 55; p. 2865-2872, ISSN: 0013-4686, doi: 10.1016/j.electacta.2009.12.059
• LIGIA M. MORETTO, THIAGO KOHLS, DENIS BADOCCO, PAOLO PASTORE, NESO SOJIC, P.UGO (2010).
Electrochemiluminescence of [Ru(bpy)3]2+ loaded in Nafion Langmuir–Blodgett films: Role of the interfacial
ultrathin film. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, vol. 640; p. 35-41, ISSN: 1572-6657, doi:
10.1016/j.jelechem.2009.12.029
• P.UGO, L.M.MORETTO, M.SILVESTRINI, F.C.PEREIRA (2010). Nanoelectrode ensembles for the direct
voltammetric determination of trace iodide in water. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL
CHEMISTRY, vol. 90; p. 747-759, ISSN: 0306-7319, doi: 10.1080/03067310902962569
• L.M. MORETTO, M. TORMEN, M. DE LEO, A. CARPENTIERO, P.UGO (2011). Polycarbonate-based ordered arrays
of electrochemical nanoelectrodes obtained by e-beam lithography. NANOTECHNOLOGY, vol. 22; p. 185305185312, ISSN: 1361-6528, doi: 10.1088/0957-4484/22/18/185305
• M.Silvestrini, P.Schiavuta, P. Scopece, G. Pecchielan, L.M. MOretto, P.UGO (2011). Modification of nanoelectrode
ensembles by thiols and disulfides to prevent non specific adsorption of proteins. ELECTROCHIMICA ACTA, vol. 56;
p. 7718-7724, ISSN: 0013-4686, doi: 10.1016/j.eleacta.2011.06.034
Personale non di ruolo nelle università o dipendenti di altri enti
1.
Cossaro, L. Floreano, A. Verdini, L. Casalis, A. Morgante, Comment on "Local Methylthiolate Adsorption
Geometry on Au(111) from Photoemission Core-Level Shifts'', Phys. Rev. Lett. 103 (11) 119601, 2009
2. M.A. Herrero, F. M.Toma, K.T. Al-Jamal, K. Kostarelos, A. Bianco, T. Da Ros, F. Bano, L. Casalis, G. Scoles, M.
Prato, “Synthesis and Characterization of a Carbon Nanotube-Dendron Series for Efficient siRNA Delivery”, J.
Am. Chem. Soc. 131 (28), pp 9843-9848 (2009)
3. F. Bano, L. Fruk, B. Sanavio, M. Glettemberg, L. Casalis, C.M. Nemeyer, G. Scoles, “Toward Multiprotein
Nanoarrays Using Nanografting and DNA Directed Immobilization of Proteins”, NanoLetters 9(7) pp 26142618 (2009)
4. G. Cellot, E. Cilia, S. Cipollone, V. Rancic, A. Sucapane, S. Giordani, L. Gambazzi, H. Markram, M. Grandolfo,
D. Scaini, F. Gelain, L. Casalis, M. Prato, M. Giugliano, L. Ballerini, “Carbon nanotubes direct interactions with
neuronal membranes ignite post spike excitability”, Nature Nanotechnology, 4, pp 126 - 133 (2008)
5. M. Castronovo, S. Radovic, C. Grunwald, L. Casalis, M. Morgante, G. Scoles, “Control of Steric Hindrance on
Restriction Enzyme Reactions with Surface-Bound DNA Nanostructures”, NanoLetters, 8 (12), pp 4140–4145
(2008)
6. E. Mirmomtaz, M. Castronovo, C. Grunwald, F. Bano, D. Scaini, A.A. Ensafi, G. Scoles, L. Casalis,
“Quantitative Study of the Effect of Coverage on the Hybridization Efficiency of Surface-Bound DNA
Nanostructures”, NanoLetters, 8 (12), pp 4134–4139 (2008)
7. Mazzarello, R. Rousseau, L. Casalis, A. Verdini, A. Kohlmeyer, L..Floreano, S. Scandolo, A. Morgante,
M.L..Klein, G. Scoles, “X-ray diffraction and computation yield the structure of alkanethiols on gold(111)”,
Science 321 (5891), pp. 943-94 (2008)
8. D. Scaini, M. Castronovo, L. Casalis, G. Scoles, “Electron transfer mediating properties of hydrocarbons as a
function of chain length: A differential scanning conductive tip atomic force microscopy investigation”, ACS
Nano 2 (3), pp. 507-515 (2008)
9. M. Pedio, B. Doyle, N. Mahne, A. Giglia, F. Borgatti, S. Nannarone, S.K.M. Henze, R. Temirov, F.S. Tautz, L.
Casalis, R. Hudej, M.F. Danisman and B.Nickel, “Growth of pentacene on Ag(111) surface: a NEXAFS study”,
Applied Surface Science 254 (1), pp. 103-107 (2007)
10. R. Mazzarello, A. Cossaro, A. Verdini, R. Rousseau, L. Casalis, M.F. Danisman, L. Floreano, S. Scandolo, A.
Morgante, G. Scoles, “Structure of a CH3S monolayer on Au(111) solved by the interplay between molecular
dynamics calculations and diffraction measurements”, Physical Review Letters 98 (1), 016102 (2007)
11. M. Castronovo, F. Bano, S. Raugei, D. Scaini, M. Dell'Angela, R. Hudej, L.Casalis, G. Scoles., “Mechanical
stabilization effect of water on a membrane-like system”, Journal of the American Chemical Society 129 (9),
pp. 2636-2641 (2007)
197
12. Corona G., Elia C., Casetta B., Da Ponte A., Del Pup L., Ottavian E., Toffoli G. Liquid Chromatography tandem
mass spectrometry assay for fast and sensitive quantification of estrone sulfate. Clin. Chim. Acta; 411: 574580, 2010.
13. Toffoli G., Cecchin E., Gasparini G., D’Andrea M., Azzarello G., Basso U., Mini E., Pessa S., De Mattia E., Lo Re
G., Buonadonna A., Nobili S., De Paoli P., Innocenti F. A genotype-driven phase I study of irinotecan
administered in combination with 5-fluorouracil/leucovorin (FOLFIRI) in metastatic colorectal cancer patients.
J. Clin. Oncol.; 28:866-71, 2010.
14. De Re V., Caggiari L., Monti G., Libra M., Spina M., Dolcetti R., De Zorzi M., Racanelli V., Crovatto M., Toffoli
G. HLA DR-DQ combination associated with the increate risk of developing HCV+ non-Hodgkin’s lymphoma is
related to the type-II mixed cryoglobulinemia. Tissue Antigens;.75:127-135, 2010.
15. De Re V., Cannizzaro R., Canzonieri V., Cecchin E., Caggiari L., De Mattia E., Pratesi C., De Paoli P., Toffoli G.
MTHFR polymorphisms on gastric cancer and on first-degree relatives of patients with gastric cancer. Tumor
Biol.; 31: 23-32, 2010.
16. Simula M.P., Cannizzaro R., Canzonieri V., Pavan A., Maiero S., Toffoli G., De Re V. PPAR signaling pathway
and cancer-related proteins are involved in celiac disease-associated tissue damage. Mol. Med.; 16: 199-209,
2010.
17. Visentin M., Biason P., Toffoli G. Drug interactions of the epidermal growth factor receptor inhibitors.
Pharmacol. and Therapeut.; 128: 82-90, 2010.
18. De Re V., Simula M.P., Notarpietro A., Canzonieri V., Cannizzaro R., Toffoli G. Is true that gliadin and tissue
transglutaminase mediate PPARg downregulation in intestinal cells of celiac disease patients? GUT; (epub
2010 Aug 30).
19. Gasparini G., D’Andrea M.R., Toffoli G. Irinotecan in the adjuvant treatment of colon cancer: is the story
finished of does personalized therapy open new opportunities? J. Clin. Oncol.; 28: e199, 2010.
20. Corona G., Elia C., Casetta B., Toffoli G. Fast liquid chromatography-tandem mass spectrometry method for
routine assessment of irinotecan metabolic phenotype. Ther. Drug Monit.; 32: 638-646, 2010.
21. Corona G., De Lorenzo E., Elia C., Simula M.P., Avellini C., Baccarani U., Lupo F., Tiribelli C., Colombatti A.,
Toffoli G. Differential proteomic analysis of hepatocellular carcinoma. Int. J. Oncol.; 36: 93-99, 2010. IF
2,447
22. De Re V., Simula M.P., Cannizzaro R., Pavan A., De Zorzi M., Toffoli G., Canzonieri V. Galectin-10,
eosinophils, and celiac disease. Ann. N. Y. Acad. Sci.; 1173: 357-364, 2009.
23. De Re V., Simula M.P., Pavan A., Garziera M., Marin D., Dolcetti R., De Vita S., Sansonno D., Geremia S.,
Toffoli G. Characterization of antibodies directed against the immunoglobulin light kappa chain variable chain
region (VK) of hepatitis C virus-related type-II mixed cryoglobulinemia and B-cell proliferations. Ann. N.Y.
Acad. Sci.; 1173:152-160, 2009.
24. Toffoli G., Biason P., Russo A., De Mattia E., Cecchin E., Hattinger C.M., Pasello M., Alberghini M., Ferrari C.,
Scotlandi K., Picci P., Serra M. Effect of TP53 Arg72Pro and MDM2 SNP309 polymorphisms on the risk of highgrade osteosarcoma development and serviva. Clin. Cancer Res.; 15(10): 3550-3556, 2009.
25. Cecchin E., Innocenti F., D’Andrea M., Corona G., De Mattia E., Biason P., Buonadanna A., Toffoli G. Predictive
role of the UGT1A1, UGT1A7 and UGT1A9 genetic variants and their hapltypes on the outcome of metastatic
colorectal cancer patients treated with fluorouracil, leucovorin, and irinotecan. J. Clin. Oncol.; 27(15):24572465, 2009.
26. Visentin M., Simula M.P., Sartor F., Petrucco A., De Re V., Toffoli G. Identification of proteins associated to
multi-drug resistance in LoVo human colon cancer cells. Int. J. Oncol.; 34(5): 1281-1289, 2009.
27. Simula M.P., Cannizzaro R., Marin M.D., Pavan A., Toffoli G., Canzonieri V., De Re V. Two-dimensional gel
proteome reference map of human small intestine. Proteome Sci; 19;(7):10, 2009.
28. Corona G., Elia C., Casetta B., Crivellari D., Rosalen S., Bari M., Toffoli G. A liquid chromatography-tandem
mass spectrometry method for the simultaneous determination of exemestane and its metabolite 17dihydroexemestane in human plasma. J. Mass Spectrom.; 44(6): 920-928, 2009.
29. De Mattia E., Toffoli G. C677T and A1298C MTHFR polymorphisms, a challenge for antifolate and
fluoropyrimidine-based therapy personalisation. Eur. J. Cancer; 45(8): 1333-1351, 2009.
30. Pavan A., Spina M., Canzonieri V., Sansonno S., Toffoli G., De Re V. Recent prognostic factors in diffuse large
B-Cell lymphoma indicate NF-KB pathway as a target for new therapeutic strategies. Leuk. Lymphoma;
49(11):2048-2058, 2008.
31. Toffoli G., De Mattia E. Pharmacogenetic relevance of MTHFR polymorphisms. Pharmacogenomics; 9(9):11951206, 2008.
32. De Re V., Simula M.P., Cannizzaro R., Toffoli G. Studio di proteomica nell’infezione da HCV: prospettive future
per l’analisi delle risposte cliniche ai nuovi farmaci. TUMORI ED INFEZIONI DA HIV. Linee guida nella terapia
di combinazione chemioterapia-HAART. GICAT. Editore: BIOMEDIA (Milano) 73- 82, 2008.
33. De Re V., De Vita S., Sansonno D., Toffoli G. Mixed cryoglobulinemia sindrome as ad additional autoimmune
disorder associated with risk for lymphoma development. Blood; 111(12):5760, 2008.
34. Biason P., Masier S., Toffoli G. UGT1A1*28 and other UGT1A polymorphisms as determinants of irinotecan
toxicity. J Chemother; 20(2):158-165, 2008.
35. Corona G., Casetta B., Sandron S., Vaccher E., Toffoli G. Rapid and sensitive analysis of vincristine in human
plasma using on-line extraction combined with liquid chromatography/tandem mass spectrometry. Rapid
Commun. Mass Spectrom.; 22(4):519-525, 2008.
36. Corona G., Vaccher E., Sandron S., Sartor I., Tirelli U., Innocenti F., Toffoli G. Lopinavir-Ritonavir
Dramatically Affects the Pharmacokinetics of Irinotecan in HIV Patients With Kaposi's Sarcoma. Clin.
Pharmacol. Ther.; 83(4):601-606, 2008.
37. De Mattia E., Cecchin E., Toffoli G. Pharmacogenetics of stomach cancer therapy. Tumori; 7(3): S66-S70,
2008.
38. De Re V., Simula M.P., Cannizzaro R., Sansonno D., Canzonieri V., Gloghini A., Carbone A., Colombatti A.,
Marin M.D., De Zorzi M., Toffoli G. Hcv inhibits antigen processing and presentation and induces oxidative
stress response in gastric mucosa.. Proteomics Clin. Appl.; 2:1290-1299, 2008.
198
39. Baldo P., Bearz A., Cannizzaro R., Canzonieri V., Dal Maso L., Di Lauro V., Lazzarini R., Milan I., Rupolo M.,
Scalone S., Spazzapan S., Toffoli G., Truccolo I., Carbone A. Interferon-alpha for follicular lymphoma
[Protocol], Cochrane Database of Systematic Reviews, 2009.
40. De Re V., Caggiari L., Dolcetti R., De Zorzi M., Simula M. P., Toffoli G. Tumori indotti da agenti infettivi:
Immunogenetica delle linfoproliferazioni HCV-associate: Il ruolo dell'HLA di classe II Biomedia, 2007.
41. De Re V., Sansonno D., De Paoli P., Geremia S., Gatti G., Caggiari L., Simula M. P., Toffoli G. Recent patents
relating to HCV molecules like putative targets for therapeutic intervention. Recent Patents on DNA & Gene
Sequences; 1: 186-194, 2007.
42. Toffoli G., Cecchin E. Pharmacogenetics and stomach cancer: an update. Pharmacogenomics; 8(5):497-505,
2007.
43. Toffoli G, De Mattia E, Cecchin E, Biason P., Masier S., Corona G. Pharmacology of epidermal growth factor
inhibitors. Int. J. Biol. Markers; 22 (1 Suppl 4):S24-S39, 2007.
44. Toffoli G, Cecchin E. Clinical implications of genetic polymorphisms on stomach cancer drug therapy.
Pharmacogenomics J.; 7(2): 76-80, 2007.
45. Toffoli G, Cecchin E, Corona G, Russo A, Buonadonna A, D'Andrea M, Pasetto LM, Pessa S, Errante D, De
Pangher V, Giusto M, Medici M, Gaion F, Sandri P, Galligioni E, Bonura S, Boccalon M, Biason P, Frustaci S.
The role of UGT1A1*28 polymorphism in the pharmacodynamics and pharmacokinetics of irinotecan in
patients with metastatic colorectal cancer. J. Clin. Oncol.; 24(19):3061-3068, 2006.
46. Toffoli G, Innocenti F. MTHFR and ALL risk: a challenge. Leuk Lymphoma; 47(7):1203-1204, 2006.
47. Corona G and Toffoli G. Interazioni farmacocinetiche tra inibitori delle protease e farmaci antiblastici:
influenza di lopinavir-ritonvair sulla farmacocinetica dell’Irinotecan. Tumori ed Infezioni da HIV, patogenesi e
studi clinici del GICAT; 75-84; Ed Biomedia, 2006.
48. Cecchin E, Corona G and Toffoli G. Reply to the letter to the editor from chowbay et al. Clin. Cancer Res.;
12(6):1942, 2006.
49. Gasparini G, Longo R, Torino F, Gattuso D, Morabito A and Toffoli G. Is tailored therapy feasible in oncology?
Crit. Rev. Oncol. Hematol.; 57(1):79-101, 2006.
50. Stocco G, Martelossi S, Sartor F, Toffoli G, Lionetti P, Barabino A, Fontana M, Decorti G, Bartoli F, Giraldi T,
Ventura A. Prevalence of methylenetetrahydrofolate reductase polymorphisms in young patients with
inflammatory bowel disease. Dig. Dis. Sci.; 51(3):474-479, 2006.
51. Crivellari D, Lombardi D, Corona G, Massacesi C, Talamini R, Sorio R, Magri MD, Lestuzzi C, Lucenti A,
Veronesi A, Toffoli G. Innovative schedule of oral idarubicin in elderly patients with metastatic breast cancer:
comprehensive results of a phase II multi-institutional study with pharmacokinetic drug monitoring. Ann.
Oncol.;17:807-812,2006.
52. Sorio R, Toffoli G, Crivellari D, Bearz A, Corona G, Colussi AM, Libra M, Talamini R, Veronesi A. Oral etoposide
in elderly patients with advanced non small cell lung cancer: a clinical and pharmacological study. J
Chemother; 18(2):188-191, 2006.
53. Toffoli G, Masier S, Biason P, Cecchin E, Corona G. Pharmacogenetics in the personalisation of Docetaxel
based chemotherapy. Tumori; 5(1):S9, 2006.
54. G. Ambrosetti, N. Johner, C. Grimaldi, T. Maeder, P. Ryser and A. Danani , "Electron Tunneling in conductorinsulator composites with spherical fillers", Journal of Applied Physics, 106, 016103 (2009)
55. Pavan G.M., Danani A., Pricl S., Smith D.K., "Modeling the Multivalent Recognition between Dendritic
Molecules and DNA: Understanding How Ligand Sacrifice and Screening Can Enhance Binding", Journal of the
American Chemical Society 2009, 131(28):9686-94
56. Posocco P., Pavan G. M., Scocchi G., Handgraaf J.-W., Malek A., Maly M., Fermeglia M., Fraaije H.J.G.E.M.,
Catapano C., Danani A., Pricl S., "Base Invaders. Coupling Experiments and Multiscale Modeling of
Dendrimer-Based siRNA Delivery Agents", Advances in Science and Technology, (2008), 57, 154-159.
57. G. Ambrosetti, N. Johner, C. Grimaldi, A. Danani, P. Ryser ,"Percolative Properties of Hard Oblate Ellipsoids of
Revolution With a Soft Shell", Phys. Rev. E 78, 061126 (2008)
58. G. Scocchi, P. Posocco, A. Danani, S. Pricl, M. Fermeglia, "To the nanoscale, and beyond! Multiscale molecular
modelling of polymer-clay nanocomposites", Fluid Phase Equilibria 261 (1-2), 366 , (2007).
59. F. Jabeen, S. Rubini, F. Martelli, A. Franciosi, A. Kolmakov, L. Gregotratti, M. Amati, A. Barinov, A. Goldoni
and M. Kiskinova, Contactless monitoring of the diameter-dependent conductivity of GaAs nanowires, Nano
Research 2, 706 (2010)
60. F. Jabeen, M. Piccin, L. Felisari, V. VGrillo, G. Bais, S. Rubini, F. Martelli, F. D'Acapito, M. Rovezzi, F.
Boscherini, Mn-induced growth of InAs nanowires, J. Vac. Sci. Technol. B 28, 478 (2010)
61. L. Wen, F. Bekisli, M. Stavola, W. B. Fowler, R. Trotta, A. Polimeni, M. Capizzi, S. Rubini, F. Martelli, Detailed
structure of the H-N-H center in GaAsyN1−y revealed by vibrational spectroscopy under uniaxial stress, Phys.
Rev. B 81, 233201 (2010)
62. R. Trotta, L. Cavigli, L. Felisari, A. Polimeni, A. Vinattieri, M. Gurioli, M. Capizzi, F. Martelli, S. Rubini, L.
Mariucci, M. Francardi, and A. Gerardino, Quantum confinement effects in hydrogen-intercalated GaAs1−xNxGaAs1−xNx :H planar, heterostructures investigated by photoluminescence spectroscopy, Phys. Rev. B 81,
235327 (2010)
63. R. Trotta, A. Polimeni, M. Capizzi, F. Martelli, S. Rubini, M. Francardi, A. Gerardino, and L. Mariucci Light
polarization control in strain-engineered GaAsN/GaAsN:H heterostructures Appl. Phys. Lett. 94, 261905
(2009)
64. G. Ciatto, F. Boscherini, A. Amore Bonapasta, F. Filippone, A. Polimeni, M. Capizzi, M. Berti, G. Bisognin, D.
De Salvador, L. Floreano, F. Martelli, S. Rubini, L. Grenouillet, Local structure of nitrogen-hydrogen complexes
in dilute nitrides Phys. Rev. B79, 165205 (2009)
65. Jabeen F., Rubini S., Martelli F., Growth of III-V semiconductor nanowires by molecular beam epitaxy
Microelectronics Journal 40, 442 (2009).
66. Jabeen F., Rubini S., Grillo V., Felisari L., Martelli F., Room temperature luminescent InGaAs/GaAs core-shell
nanowires Appl. Phys. Lett. 93, 083117 (2008)
199
67. Felisari L., Grillo V., Martelli F., Trotta R., Polimeni A., Capizzi M., Jabeen F., Mariucci L., In-plane band gap
modulation investigated by secondary electron imaging of GaAsN/GaAsN:H heterostructures Appl. Phys. Lett.
93, 102116 (2008)
68. Trotta R., Polimeni A., Capizzi M., Giubertoni D., Bersani M., Bisognin G., Berti M., Rubini S., Martelli F.,
Mariucci L., Francardi M., Gerardino A., Effect of hydrogen incorporation temperature in in plane-engineered
GaAsN/GaAsN:H heterostructures Appl. Phys. Lett. 92, 221901 (2008)
69. Jabeen F., Grillo V., Rubini S., Martelli F., Self-catalyzed growth of GaAs nanowires on Si by molecular beam
epitaxy Nanotechnology 19 , 275711 (2008)
70. Bisognin G., De Salvador D., Napolitani E., Berti M., Polimeni A., Capizzi M., Rubini S., Martelli F., Franciosi
A., High-resolution X-ray diffraction in situ study of very small complexes: The case of hydrogenated dilute
nitrides Journal of Applied Crystallography 41 , 366 (2008)
71. Polimeni A., Pettinari G., Trotta R., Masia F., Felici M., Capizzi M., Lindsay A., O'Reilly E. P., Niebling T., Stolz
W., Klar P. J., Martelli F., Rubini S., Photoluminescence under magnetic field and hydrostatic pressure for
probing the electronic properties of GaAsN Physica Status Solidi (A) 205, 107 (2008)
72. Kleekajai S., Jiang F., Colon K., Stavola M., Fowler W. B., Martin K. , R., Polimeni A., Capizzi M., Hong Y. G.,.
Xin H. P, Tu C. W., Bais G., Rubini S., Martelli F., Vibrational properties of the H-N-H complex in dilute III-N-V
alloys: Infrared spectroscopy and density functional theory Physical Review B77 , 085213 (2008)
73. Berti M., Bisognin G., De Salvador D., Napolitani E., Vangelista S., Polimeni A.,Capizzi M., Boscherini F.,
Ciatto G., Rubini S., Martelli F., Franciosi A., Formation and dissolution of D-N complexes in dilute nitrides
Phys. Rev. B 76, 205323 (2007)
74. Bisognin G., De Salvador D., Napolitani E., Berti M., Polimeni A., Felici M., Capizzi M., Bais G., Jabeen F.,
Piccin M., Rubini S., Martelli F., Franciosi A., Thermal evolution of small N–D complexes in deuterated dilute
nitrides revealed by in-situ high resolution X-ray diffraction phys. stat. sol. (a) 204, 2766 (2007)
75. Martelli F., Piccin M.,. Bais G, Jabeen F., Ambrosini S., Rubini S., Franciosi A., Photoluminescence of Mncatalyzed GaAs nanowires grown by molecular beam epitaxy Nanotechnology, 18, 125603 (2007).
76. Geddo M., Ciabattoni T., Guizzetti G., Galli M., Patrini M., Polimeni A., Trotta R., Capizzi M., Bais G., Piccin M.,
Rubini S., Martelli, F., Franciosi A., Photoreflectance and Reflectance investigation of deuterium irradiated
GaAsN, Appl. Phys. Lett. 90, 091907 (2007).
77. Rubini S., Piccin M., Bais G., Jabeen F., Martelli F., Franciosi A., GaAs nanowires by Mn-catalysed molecular
beam epitaxy Journal of Physics: Conference Series 61, 992 (2007)
78. Piccin M., Bais G., Grillo V., Jabeen F., De Franceschi S., Carlino E., Lazzarino M., Romanato F., Businaro L.,
Rubini S. , Martelli F., Franciosi A., Growth by molecular beam epitaxy and electrical characterization of GaAs
nanowires Physica E 37, 134 (2007).
79. Masia F., Pettinari G., Polimeni A., Felici M., Miriametro A., Capizzi M., Lindsay A., Healy S. B., O'Reilly E. P.,
Cristofoli A., Bais G., Piccin M., Rubini S., Martelli F., Franciosi A., Klar P. J., Volz K., and Stolz W., Interaction
between conduction band edge and nitrogen states probed by carrier effective mass measurements in GaAs1xNx Phys. Rev. B73, 077201 (2006).
80. Felici M., Polimeni A., Salviati G., Lazzarini L., Armani N., Masia F., Capizzi M., Martelli F., Lazzarino M., Bais
G., Piccin M., Rubini S., Franciosi A. ,In-plane band gap engineering by modulated hydrogenation of dilute
nitride semiconductors Adv. Mater. 18, 1993 (2006).
81. Martelli F., Rubini S., Piccin M., Bais G., Jabeen F., De Franceschi S., Grillo V., Carlino E., D'Acapito F.,
Boscherini F., Cabrini S., Lazzarino M., Businaro L., Romanato F., Franciosi A., Mn induced growth of GaAs
nanowires, Nano Letters, 6, 2130 (2006).
82. Frassanito M.C., De Giorgi M., Rinaldi R., Cingolani R., Rubini S., Piccin M., Cristofoli A., Bais G., Martelli F.,
Carlino E., Franciosi A., Microphotoluminescence characterization of alloy fluctuations in InGaAsN/GaAs
quantum wells emitting at 1.3?m Semic. Sci. Technol. 21, 1207 (2006)
83. Bisognin G., De Salvador D., Drigo A.V., Napolitani E., Sambo A., Berti M., Polimeni A., Felici M., Capizzi M.,
Güngerich M., Klar P.J., Bais G., Jabeen F., Piccin M., Rubini S., Martelli F., Franciosi A., Hydrogen-nitrogen
complexes in dilute nitride alloys: origin of the compressive lattice strain Appl. Phys. Lett. 89, 061904 (2006).
84. Carlino E., Martelli F., Rubini S., Franciosi A., Catalyst incorporation in ZnSe nanowires Phil. Mag. Lett. 86,
261 (2006).
85. Rubini S., Bais G., Cristofoli A., Piccin M., Duca R., Nacci C., Modesti S., Carlino E. , Martelli F. Franciosi A.,
Nitrogen induced hindering of In incorporation in InGaAsN Appl. Phys. Lett. 88, 141923 (2006).
86. Pelucchi E., Kumar D., Lazzarino M., Rubini S., Franciosi A., Controlling interface reactivity and Schottky
barrier height in Au/ZnSe (001) junctions, J. Vac. Sci. Technol. B24, 1259 (2006).
87. D’Acapito F., Smolentsev G., Boscherini F., Piccin M., Bais G., Rubini S., Martelli F., Franciosi A., Site of Mn in
d-doped GaAs: X-ray absorption spectroscopy Phys. Rev. B73, 035314 (2006)
88. Roveda L, Zonta A, Staffieri F, Timurian D, DiVenere B, Bakeine GJ, Crovace A, Prati U., Experimental
modified orthotopic piggy-back liver autotransplantation, Appl Radiat Isot. 2009 Jul;67(7-8 Suppl):S306-8.
Epub 2009 Mar 27.
89. Zonta A, Pinelli T, Prati U, Roveda L, Ferrari C, Clerici AM, Zonta C, Mazzini G, Dionigi P, Altieri S, Bortolussi
S, Bruschi P, Fossati F., Extra-corporeal liver BNCT for the treatment of diffuse metastases: what was learned
and what is still to be learned, Appl Radiat Isot. 2009 Jul;67(7-8 Suppl):S67-75. Epub 2009 Mar 28.
90. Altieri S, Bortolussi S, Bruschi P, Chiari P, Fossati F, Stella S, Prati U, Roveda L, Zonta A, Zonta C, Ferrari C,
Clerici A, Nano R, Pinelli T., Neutron autoradiography imaging of selective boron uptake in human metastatic
tumours, Appl Radiat Isot. 2008 Dec;66(12):1850-5. Epub 2008 May 29.
91. Asteria CR, Gagliardi G, Pucciarelli S, Romano G, Infantino A, La Torre F, Tonelli F, Martin F, Pulica C, Ripetti
V, Diana G, Amicucci G, Carlini M, Sommariva A, Vinciguerra G, Poddie DB, Amato A, Bassi R, Galleano R,
Veronese E, Mancini S, Pescio G, Occelli GL, Bracchitta S, Castagnola M, Pontillo T, Cimmino G, Prati U,
Vincenti R., Anastomotic leaks after anterior resection for mid and low rectal cancer: survey of the Italian
Society of Colorectal Surgery., Tech Coloproctol. 2008 Jun;12(2):103-10. Epub 2008 Jun 10.
200
92. Bakeine GJ, Bertolotti A, Latina M, Congiu T, Prati U, Roveda L, Trotta F, Tormen M, Fabrizio ED, Carlini G,
Facoetti A, Nano R., Surface properties and implantation site affect the capsular fibrotic overgrowth, J Biomed
Mater Res A. 2007 Dec 15;83(4):965-9.
93. Paraskevopoulou, C., Fairhurst, S.A., Lowe, D.J., Brick, P. & Onesti, S. (2006). The Elongator subunit Elp3
contains a Fe4S4 cluster and binds S-adenosylmethionine. Mol. Microbiol. 59, 795-806.
94. Palmieri, G., Casbarra, A., Fiume, I., Catara, G., Capasso, A., Marino, G., Onesti, S. & Rossi, M. (2006)
Identification of the first archaeal oligopeptide binding protein from the hyperthermophile Aereopyrum pernix.
Extremophiles, 10, 393-402.
95. Costa A., Pape T., van Heel M., Brick P., Patwardhan A. & Onesti S. (2006a) Structural studies of the archaeal
MCM complex in different functional states. J. Struct. Biol., 156, 210-219.
96. Costa A., Pape T., van Heel M., Brick P., Patwardhan A. & Onesti S. (2006b). Structural basis of the
Methanobacter thermautotrophicus MCM helicase activity. Nucleic Acid Res. 34, 5829-5838.
97. Vinaya Sampath V., Bindu Balakrishnan B., Verma-Gaur J., Onesti S. & Sadhale P.P. (2008) Unstructured N
terminus of Rpb4 contributes to the interaction of Rpb4/Rpb7 subcomplex with the core RNA polymerase II in
S. cerevisiae. J. Biol. Chem., 283, 3923-3931.
98. Costa A & Onesti, S. (2008). The MCM complex: (just) a replicative helicase? Biochem. Trans. 36, 136-140.
99. Costa A., van Dujnen G., Medagli B., Chong J., Sakakibara N., Kelman Z., Nair S.K., Patwardhan A. & Onesti
S. (2008). Cryo-electron microscopy reveals a novel DNA binding site on the MCM helicase. EMBO J., 27,
2250-2258.
100.Bae B., Chen Y.-H., Costa A., Onesti S., Brunzelle J.S., Lin Y., Cann I.K.O. & Nair S.K. (2009) Crystal
Structure of an Archaeal MCM Homolog Provides Insights into the Architecture of the Replicative Helicase.
Structure 17, 211-222.
101.Jenkinson, E.R., Costa A., Leech, A.P., Patwardhan A., Onesti S. & Chong, J.P (2009). Mutations in subdomain B of the MCM helicase affect DNA binding and modulate conformational transitions. J. Biol. Chem.
284, 5654-5661.
102.Costa, A & Onesti, S. (2009). Structural biology of MCM helicases. Crit. Rev. Biochem. Mol. Biol. 44, 326-342.
103.M. Lazzarino, E. De Marchi, M. Bressanutti, L. Vaccari, S. Cabrini, C. Schmid, R. Poetes R and G. Scoles, Twin
cantilevers with a nanogap for single molecule experimentation. Microelectronic Eng., 83, 1309 (2006)
104.E. Pelucchi, D. Kumar, M. Lazzarino, S. Rubini and A. Franciosi, Controlling interface reactivity and Schottky
barrier height in Au/ZnSe(001) junctions. J. Vac. Sci. Technol/ B 24, 1259 (2006)
105.M. Felici, A. Polimeri, G. Salviati, L. Lazzaroni, N. Armani, F. Masia, M. Capizzi, F. Martelli, M. Lazzarino, G.
Bais, M. Piccin, S. Rubini and A. Franciosi, In-plane bandgap engineering by modulated hydrogenation of
dilute nitride semiconductors. Adv. Mat., 18, 1993 (2006)
106.F. Martelli, S. Rubini,M. Piccin, G. Bais, F. Jabeen, S. Defranceschi, V. Grillo, E. Carlino, F. D'Acapito, F.
Boscherini, S. Cabrini, M. Lazzarino, L. Businaro, F. Romanato, and A. Franciosi, Manganese-induced growth
of GaAs nanowires. Nanoletters, 6, 2130 (2006)
107.M. Lazzarino, G. Mori, L. Sorba, D. Ercolani, G. Biasiol, S. Heun and A. Locatelli, Chemistry and formation
process of Ga(Al)As oxide during local anodic oxidation nanolithography, Surf. Sci., 600, 3739 (2006)
108.M. Piccin, G. Bais, V. Grillo, F. Jabeen, S. De Franceschi, E. Carlino, M. Lazzarino, F. Romanato, L. Businaro,
S. Rubini, F. Martelli and A. Franciosi, Growth by molecular beam epitaxy and electrical characterization of
GaAs nanowires. Physica E doi.org/10.1016/j.physe.2006.07.002
109.Qazi, D. Nonis, A. Pozzato, M. Tormen, M. Lazzarino, S. Carrato and G. Scoles, Symmetric twin cantilever for
single molecole detection. Appl. Phys. Lett, *90*, 173118 (2007)
110.Maione, IA; Macucci, M; Iannaccone, G; Basso, G; Pellegrini, B; Lazzarino, M; Sorba, L; Beltram, F. , Probing
Pauli blocking with shot noise in resonant tunneling diodes: Experiment and theory. Phys. Rev. B, 75, 125327
(2007)
111.V Toffoli, F Esch, M Melli, F Cataruzza, A Pozzato, S Carrato, G Scoles, M Tormen and M Lazzarino,
Intrinsically aligned chemo-mechanical functionalization of twin cantilever structure., Nanotechnology, 19
445502 (2008)
112.Kondra, S., Laishram, J., Ban, J., Migliorini, E., Di Foggia, V., Lazzarino, M., Torre, V., Ruaro, M.E.,
Integration of confocal and atomic force microscopy images, Journal of Neuroscience, Methods, 177. 94
(2009)
113.V Toffoli, F Esch, M Melli, A Pozzato, M Tormen and M Lazzarino, Chemical functionalization of atomically flat
cantilever surfaces, Microelectronic Eng., 86, 1200 (2009)
114.M.Polano, A. Bek, F. benetti, M. Lazzarino and G. Legname, Structural Insights into Alternate Aggregated
Prion Protein Forms, J. Mol. Biol, 393, 1033 (2009)
115.A morphological analysis of growth cones of DRG neurons combining Atomic Force and Confocal J. Laishram,
S. Kondra, D. Avossa, E. Migliorini, M. Lazzarino and V. Torre, Microscopy. Atomic Force and Confocal
Microscopy. J. Struct. Biol., 168, 366 (2009)
116.Tip enhanced Raman scattering with adiabatic plasmon focusing tips.
117.Bek, A., De Angelis, F., Das, G., Di Fabrizio, E., Lazzarino, M. Micron Article in Press (2010)
118.Inverted tapered pillars for mass sensing. Melli, M., Cozzato, A., Lazzarino, M. 2010 Microelectronic
Engineering 87, 730 (2010)
119.Modulation of alpha-synuclein aggregation by dopamine analogs.
120.Latawiec, D., Herrera, F., Bek, A., Losasso, V., Candotti, M., Benetti, F., Cino, E. M. Lazzarino, P. Carloni and
Legname, G. PLoS ONE 5 (2), art. no. e9234 (2010)
121.Nanoscale chemical mapping using three-dimensional adiabatic compression of surface plasmon polaritons .
De Angelis, F., Das, G., Cloro, P., Patrini, M., Galli, M., Bek, A., Lazzarino, M., (...), Di Fabrizio, E. Nature
Nanotechnology 5 67 (2010)
122.Fragmentation as a mechanism for growth cone pruning and de generation. Jelena Ban, Elisa Migliorini,
Valentina Di Foggia, Marco Lazzarino, Maria Elisabetta Ruaro, Vincent Torre. Stem Cells and Development.
Sep 2010, ahead of print.
201
123.D. Cojoc, “Optical manipulation in support of high spatial and temporal resolution experiments in Biophysics”,
Invited Review, SPIE Reviews, Fall 2009.
124.V. Garbin, B. Dollet, M. Overvelde, D. Cojoc, E. Di Fabrizio, L. Van Wijngaarden, A. Prosperetti, N. de Jong, D.
Lohse, and M. Versluis “History force on coated microbubbles propelled by ultrasound" , Physics of Fluids (July
2009).
125.D. Cojoc, H. Amenitsch, C. Riekel, M. Rappolt, M. Burghammer, S. Santucci, G. Grenci, B. Sartori, B.
Marmiroli, “X-ray microdiffraction of microsamples manipulated in fluidic environment without mechanical
contact”, ESRF Highlights 2008 48-49 http://www.esrf.eu/files/Highlights/HL2008.pdf .(2009)
126.V. Garbin, G. Volpe, E. Ferrari, M. Versluis, D. Cojoc and D. Petrov, “Mie scattering distinguishes the
topological charge of an optical vortex: a homage to Gustav Mie”, New Journal of Physics, 11, 013046,
(2009).
127.E. Connell, A. Giniatullina, J.L.K. Him, P. Scott, E. Ferrari, A. Roseman, D. Cojoc, A.R. Brisson and B.
Davletov, “Cross-linking of Phospholipid Membranes is a Conserved Property of Calcium-Sensitive,
Synaptotagmins”, J. Mol. Biol. 380 42 (2008).
128.M. Mihailescu, A. Preda, D. Cojoc, E. Scarlat, L. Preda, “Diffractive patterns from a phyllotaxis type
arrangement”, Opt. Laser Eng. 46, 802-809, (2008).
129.J. Garcia, V. Mico, D. Cojoc and Z. Zalevsky, “Full field of view superresolution imaging based on two static
gratings and white illumination”, Appl. Opt. 47(17) 3080 (2008).
130.D. Cojoc, E. Ferrari, V. Garbin, E. Di Fabrizio, H. Amenitsch, M. Rappolt, B. Sartori, P. Laggner, M.
Burghammer, C. Riekel, “Scanning x-ray microdiffraction of optically manipulated liposomes”, Appl. Phys.
Lett. 91 234107 (2007).
131.D. Cojoc, E. Ferrari, F. Difato, R. Shahapure, J. Laishram, M. Righi, E. Di Fabrizio, Vincent Torre, "Properties
of the force exerted by filopodia and lamellipodia and the involvement of cytoskeletal components", PLoS ONE
2(10): e1072. doi:10.1371/journal.pone.0001072 (2007).
132.H. Amenitsch, E. Ferrari, M. Rappolt, B. Sartori, P. Laggner, V. Garbin, E. Di Fabrizio, M. Burghammer, C.
Riekel, D. Cojoc “Development of optical tweezers for sample fixing in microdiffraction experiments” ESRF,
Newsletter 45 26 (2007)
133.M. Mihailescu, A. Preda, D. Cojoc, L. Preda E. Scarlat, I.M. Popescu, “Intensity redistribution in diffractive
pattern due to fractal phase changes”, J. Optoel. Adv. Mat. June 2007.
134.A.R. Moradi, E. Ferrari, V. Garbin, E. Di Fabrizio, D. Cojoc, “Strength control in multiple optical traps
generated by means of diffractive optical elements”,.Optoelectronics and Advanced Materials – Rapid
Communications 1 158 (2007).
135.V. Garbin, D. Cojoc, E. Ferrari, E. Di Fabrizio, M. Overvelde, M. Versluis, S. van der Meer, N. de Jong, D.
Lohse “Changes in microbubble dynamics near a boundary revealed by combined optical micromanipulation
and high-speed imaging”, Appl. Phys. Lett. 90 114103 (2007).
136.M. Mihailescu, A. Preda, D. Cojoc, E. Scarlat, L. Preda, “Diffractive patterns correlation with shape and
structure of imprint objects”, J. Optoel. Adv. Mat. 9 1071 (2007).
137.Alexandrescu, D. Cojoc, and E. Di Fabrizio, “Mechanism of Angular Momentum Exchange between Molecules
and Laguerre-Gaussian Beams”, Phys. Rev. Lett. 96 243001 (2006)
138.D. Cojoc, B. Kaulich, A. Carpentiero, S. Cabrini, L. Businaro, E. Di Fabrizio, “X-ray vortices with high
topological charge”, Microelectron. Eng. 83 1360 (2006).
139.S. Cabrini, C. Liberale, D. Cojoc, A. Carpentiero, M. Prasciolu, S. Mora, V. Degiorgio, F. De Angelis, E. Di
Fabrizio, “Axicon lens on optical fiber forming optical tweezers, made by focused ion beam milling”,
Microelectron. Eng. 83 804 (2006).
140.D. Cojoc, A. Carpentiero, M. Prasciolu, E Di Fabrizio, L. Businaro, B. Kaulich, “Fabrication of novel diffractive
optical elements for x-ray microscopy”, Elettra Highlights 2006 133 (2006).
141.Cecchin E, Agostini M, Pucciarelli S, De Paoli A, Canzonieri V, Sigon R, De Mattia E, Friso ML, Biason P,
Visentin M, Nitti D, Toffoli G: Tumor response is predicted by patient genetic profile in rectal cancer patients
treated with neo-adjuvant chemo-radiotherapy. The Pharmacogenomics J; 6 April 2010 [Advanced on-line
publication].
142.Corona G, De Lorenzo E, Elia C, Simula MP, Avellini C, Baccarani U, Lupo F, Tiribelli C, Colombatti A, Toffoli G.
Differential proteomic analysis of hepatocellular carcinoma. Int J Oncol; 36:93-9, 2010.
143.Simula MP, Cannizzaro R, Canzonieri V, Pavan A, Maiero S, Toffoli G, De Re V. PPAR signalling pathway and
cancer-related proteins are involved in celiac disease-associated tissue damage. Mol Med, 16: 199-209, 2010.
144.Visentin M, Biason P, Toffoli G. Drug interactions among the epidermal growth factor receptor inhibitors, other
biologic and cytotoxic agents. Pharmacol Ther, 128: 82-90, 2010.
145.Corona G, Elia C, Casetta B, Toffoli G. fast liquid chromatography tandem mass spectrometry method for
routine assessment of irinotecan metabolic phenotype. Ther Drug Monit; 32: 638-46, 2010.
146.De Re V, Simula MP, Notarpietro A, Canzonieri V, Cannizzaro R, Toffoli G. Do gliadin and tissue
transglutaminase mediate PPAR downregulation in intestinal cells of patients with coeliac disease? Gut; 59:
1730-1. 2010
147.Corona G, Elia C, Casetta B, Da Ponte A, Del Pup L, Ottavian E, Toffoli G. Liquid Chromatography tandem
mass spectrometry assay for fast and sensitive quantification of estrone sulfate. Clin. Chim. Acta; 411: 574580, 2010.
148.Toffoli G, Cecchin E, Gasparini G, D’Andrea M, Azzarello G, Basso U, Mini E, Pessa S, De Mattia E, Lo Re G,
Buonadonna A, Nobili S, De Paoli P, Innocenti F. A genotype-driven phase I study of irinotecan administered
in combination with 5-fluorouracil/leucovorin (FOLFIRI) in metastatic colorectal cancer patients. J. Clin.
Oncol.;28: 866-71, 2010.
149.De Re V., Caggiari L., Monti G., Libra M., Spina M., Dolcetti R., De Zorzi M., Racanelli V., Crovatto M., Toffoli
G. HLA DR-DQ combination associated with the increate risk of developing HCV+ non-Hodgkin’s lymphoma is
related to the type-II mixed cryoglobulinemia. Tissue Antigens;.75:127-135, 2010.
202
150.De Re V, Cannizzaro R, Canzonieri V, Cecchin E, Caggiari L, De Mattia E, Pratesi C, De Paoli P, Toffoli G.
MTHFR polymorphisms on gastric cancer and on first-degree relatives of patients with gastric cancer. Tumor
Biol.; 31: 23-32, 2010.
151.Mechanical stabilization effect of water on a membrane-like system, M. Castronovo, F. Bano, S. Raugei, D.
Scaini, M. Dell'Angela, R. Hudej, L. Casalis, G. Scoles, J. Am. Chem. Soc. 129, 2636-2641, (2007)
152.Characterization of indium tin oxide surfaces after KOH and HCl treatments, S. Gardonio, L. Gregoratti, D.
Scaini, C. Castellarin-Cudia, P. Dudin, P. Melpignano, V. Biondo, R. Zamboni, S. Caria, M. Kiskinova, Organic
Electronics 9, 253-261, (2008)
153.Electron Transfer Mediating Properties of Hydrocarbons as a Function of Chain Length: A Differential Scanning
Conductive Tip Atomic Force Microscopy Investigation, D. Scaini, M. Castronovo, L. Casalis, G. Scoles, ACS
Nano, 2, 507-515, (2008)
154.MgO-Supported Rhodium Particles and Films: Size, Morphology, and Reactivity, L. Gregoratti, D. Scaini, Y.
He, H. Over, M. Kiskinova, J. Phys. Chem. C, 112, 9040-9044, (2008)
155.Primate cathelicidin orthologues display different structures and membrane interactions, F. Morgera, L.
Vaccari, N. Antcheva, D. Scaini, S. Pacor, A. Tossi, Biochem J., 417, 727-735, (2008)
156.Quantitative Study of the Effect of Coverage on the Hybridization Efficiency of Surface-Bound DNA
Nanostructures, E. Mirmomtaz, M. Castronovo, C. Grunwald, F. Bano, D. Scaini, A. Ensafi, G. Scoles, L.
Casalis, Nano Lett., 8, 4134-4139 (2008)
157.Carbon nanotubes might improve neuronal performance by favouring electrical shortcuts, G. Cellot, E. Cilia,
S. Cipollone, V. Rancic, A. Sucapane, S. Giordani, L. Gambazzi, H. Markram, M. Grandolfo, D. Scaini, F.
Gelain, L. Casalis, M. Prato, M. Giugliano, L. Ballerini, Nature Nanotech.,4, 126-133 (2009)
158.Oriented Immobilization of Prion Protein Demonstrated via Precise Interfacial Nanostructure Measurements,
Barbara Sanavio, Denis Scaini, Christian Grunwald, Giuseppe Legname, Giacinto Scoles, Loredana Casalis,
ACS Nano, 4, 6607-6616, (2010)
159.Inverted tapered pillars for mass sensing. Melli M., Pozzato A., Lazzarino M. 2010 /Microelectronic
Engineering/ *87*, 730 (2010)
160.Modulation of alpha-synuclein aggregation by dopamine analogs. Latawiec, D., Herrera, F., Bek, A., Losasso,
V., Candotti M., Benetti F., Cino E. M. Lazzarino P. Carloni and Legname G. /PLoS ONE/ 5 (2), art. no. e9234
(2010)
161.Nanoscale chemical mapping using three-dimensional adiabatic compression of surface plasmon polaritons .
De Angelis F., Das G., Candeloro P., Patrini M., Galli M., Bek A., Lazzarino M. , Di Fabrizio E. /Nature
Nanotechnology/ *5 * 67 (2010)
203
7.Criteri di valuatazione delle scuole di dottorato
Nell’attesa dell’emanazione del nuovo decreto di disciplina del dottorato di ricerca (ex art. 19 L.
240/2010), il Nucleo di Valutazione ha stabilito di utilizzare nella valutazione delle proposte di
Corsi e Scuole di Dottorato per l’anno 2012 (XXVIII ciclo) il medesimo modello di valutazione
utilizzato lo scorso anno, salvo nuove indicazioni operative che dovessero pervenire dal MIUR.
RIFERIMENTI NORMATIVI DM.224 Nota DM.50/2010 /99 art.2 MIUR Programmazio
ne 2010-2012 n.640/2 INDICATORI E RANGE GIUDIZI INDIRI
ZZI MIUR 011 punti a) b) ‐ comma 3 lett.
a), b), c) (ovvero 1° - 2° - 3° Requisit o) punto c) comma 3 lett. d) (ovvero 4° Requisit o) punto B12 1 Qualità
scientifi
ca, elevati
requisit
i di struttur
ee risorse
per la
ricerca 2 Impatt
o dottora
to – mondo produtt
ivo a) Composizione collegio docenti N docenti esclusivi nei SSD del corso b) Produzione scientifica del collegio docenti Valutazione
c) Pubblicazioni scientifiche dei dottorandi Valutazione
d) Iniziative di pubblicizzazione delle tesi di dottorato Valutazione
POSITIVO / NEGATIVO (è obbligatorio per tutti
OpenstarTS) e) Disponibilità risorse e strutture Valutazione
INSUFF – SUFF – BUONO ‐ OTTIMO f) Livello di soddisfazione dei dottorandi % soddisfatti questionario dottorandi 2010 />= 50% INSUFF 35%< =/ <50% SUFF 20%<= / <35% BUONO /<20% OTTIMO a) Inserimento lavorativo dottori di ricerca (monitoraggio annuale inviato al NV) % dottori con inserimento lavorativo connesso al titolo ultimi 6 anni %<=30 o non fornito => INSUFF 30< % <=50 => SUFF 50< % <=70 => BUONO % >70 => OTTIMO b) Cofinanziamento delle borse previsto per il XXVII ciclo % borse finanziate con fondi privati italiani e stranieri % <= 10 BASSO 10 < % <= 30 MEDIO % > 30 ALTO c) Fonte di finanziamento dei progetti di ricerca attivi nei dipartimenti proponenti % di fondi provenienti da privati italiani e stranieri Scuola di dottorato di ricerca in Nanotecnologie: attività 2013
N >= min => ADEGUATO N < min => NON ADEGUATO INSUFF – SUFF – BUONO ‐ OTTIMO INSUFF – SUFF – BUONO ‐ OTTIMO % <= 10 BASSO 10 < % <= 30 MEDIO % > 30 ALTO 204
RIFERIMENTI NORMATIVI DM.224 Nota /99 art.2 MIUR n.640/2 011 punto d) DM.50/20 INDICATORI E RANGE GIUDIZI INDIRIZZI
MIUR 10 Program
mazione 20102012 punto B12 3 a) Attrattività dottorato Razionalizza
zione dei
corsi b) Attrattività dottorato sulle altre sedi comma 3 lett. b) (ovvero 2° Requisit o) c) Apertura sede N candidati presenti alla prova/ N posti con borsa banditi N candidati laureati altro ateneo presenti alla prova / N totale candidati presenti alla prova N iscritti dottorato provenienti da altri atenei / N iscritti totali d) Dimensione prevista del dottorato N posti ordinari XXVII e) Dimensione effettiva del dottorato media degli iscritti negli ultimi 3 cicli (confrontata con posti e borse previsti) comma 2 comma 3 lett.
a), e) (ovvero 1° - 5° Requisit o) punto e) ‐ 4 Ampiezza
delle tematiche scientifiche
con riferimento a
discipline riconoscibili
a livello internazional e a) Denominazione chiara e traducibile Valutazione
b) Coerenza tra tematiche scientifiche e denominazione Valutazione
c) Ampiezza tematiche scientifiche N SSD di riferimento del corso d) Adeguata formulazione delle tematiche scientifiche e degli obiettivi formativi Valutazione
N < 2 BASSA 2 <= N <= 4 MEDIA N > 4 ALTA % <= 10 BASSA 10 < % <= 25 MEDIO‐BASSA 25 < % <= 45 MEDIA 45 < % <= 60 MEDIO‐ALTA % > 60 ALTA % <= 10 BASSA 10 < % <= 25 MEDIO‐BASSA 25 < % <= 45 MEDIA 45 < % <= 60 MEDIO‐ALTA % > 60 ALTA ADEGUATA – NON ADEGUATA (con riferimento al tipo corso e agli iscritti effettivi) ADEGUATA – NON ADEGUATA (con riferimento al tipo corso e al numero delle borse) ADEGUATA – NON ADEGUATA SI – NO ADEGUATA se N >= 3 NON ADEGUATA se N < 3 ADEGUATA – NON ADEGUATA 205
RIFERIMENTI NORMATIVI INDICATORI E RANGE GIUDIZI INDIRIZZI
MIUR DM.224 Nota DM.50/20 /99 art.2 MIUR 10 n.640/2 Program
011 mazione 20102012
comma 3 lett. d) (ovvero 4° Requisit o) punto f) ‐ 5 Internaziona
lizzazione a) Convenzioni con soggetti esteri per attività formative dottorandi b) Fonte di finanziamento dei progetti di ricerca attivi nei dipartimenti proponenti c) Presenza di iscritti stranieri d) Sito web del dottorato Valutazione
PREVISTE – NON PREVISTE % di fondi provenienti da soggetti stranieri N iscritti stranieri / N iscritti totali Valutazione % <= 20 BASSO 20 < % <= 40 MEDIO % > 40 ALTO % < 10 BASSO 10 <= % < 20 MEDIO % > =20 ALTO INSUFFICIENTE ‐ SCARSO –
SUFFICIENTE – DISCRETO – BUONO – MOLTO BUONO – OTTIMO comma 3 lett. f) ‐ (ovvero 6° Requisit o) ‐ 6 Adozione di sistemi di valutazione Valutazione
ADEGUATO – NON ADEGUATO Sistemi di Valutazione Scuola di dottorato di ricerca in Nanotecnologie: attività 2013
206
8. Giudizi dei referee esterni per dottorandi ammessi all’esame finale
aprile 2013
Di seguito sono riportati i referee esterni scelti dal collegio per ciascuno dei dottorandi:
Studente
Supervisore
AMBROSINI RUBINI
Stefano
Silvia
Titolo
Reviewers
Growth and characterization Anna Fontcuberta i Morral, École
of nanostructures.
polytechnique fédérale de Lausanne
Helge Weman, Norwegian University
of Science and Technology
CHEN Yan FORNASIE Nanostructured
Titanium Giovanni Zangari, University of
Xin
RO Paolo
Dioxide Based Materials for Virginia
Photocatalysis
and Hansjörg Grützmache, ETH Zürich
Phototelectrocatalysis
BIDOGGIA
PASQUATO Mixed-monolayers
Fabrizio Mancin, Università degli
Silvia
Lucia
protected gold nanoparticles Studi di Padova,
for applications in medicine Durand
Grégory,
University
d’Avignon,
GANAU
SCOLES
Nanotechnology
Milan Skrap, Università di Udine
Mario
Giacinto
applications in quantitative Francesco Stellacci, EPFL, Lausanne,
neuroscience:
proteomic Switzerland
analyses
of
malignant
gliomas
GIORGIS
ROMANAT Design, fabrication and Filiberto Bilotti, Università di Roma 3
Valentina
O Filippo
characterization
of Jakub Dostalek, Austrian Institute of
metamaterials
inspired Technology
plasmonic structures for
sensing application
LUCAFO'
SAVA
Study
of
carbon Christian Gaiddon, Centre National de
Marianna
Gianni
nanostructures as carriers la Recherche Scientifique, France
for drugs for cancer Paul J Dyson, EPFL, Lausanne,
chemotherapy.
Switzerland
MARSICH
SERGO
Design and synthesis of Peter
HIldebrandt,
Technische
Lucia
Valter
functionalized
metal Universitaet Berlin
nanoparticles
for
bio- Freek
Ariese,Vrije
Universiteit
analysis
with
Surface- Amsterdam
Enhanced Raman Scattering
(SERS)
PERONIO
COMELLI
Single-molecule
Gian Paolo Brivio, Università'
Angelo
Giovanni
heterogeneous catalysis
Bicocca di Milano.
Saw Wai Hla, Argonne National
Laboratory
SAMMITO
ROMANAT Integration of plasmonic Theodoros Dimopoulos, Austrian
Davide
O Filippo
gratings into optoelectronic Institute of Technology
devices
Emiliano Descrovi, Poitecnico di
Torino
ZANUSSO
TOFFOLI
Nanotechnologies
and Enrico Mini, Università di Firenze.
Chiara
Giuseppe
oncology: pharmacokinetics Emilio Clementi, Università di
207
and pharmacogenomics to Milano.
optimize the antitumor
therapies
Vedi dettaglio negli allegati.
208
9.Presentazioni dei dottorandi al congesso del gennaio 2013
Le presentazioni PPT dei dottorandi al congresso del gennaio 2012 sono riportate negli allegati.
209