Acronimo: PSYCHROnucleation
Transcript
Acronimo: PSYCHROnucleation
Sandra Pucciarelli, PhD Address: School of Biosciences and Biotechnology, University of Camerino, Via Gentile III da Varano, Camerino, MC, Italy. Present position: Assistant professor Area of research: Ecology Birth date: 23/10/1969 Languages: Italian (mother tongue); English (fluent); French (fluent). EDUCATION AND WORK EXPERIENCES Master Degree in Biology: 110/110 cum laude. Tutor: Prof. C. Miceli, University 22-07-1993 of Camerino, Italy. Doctorate Degree in "Biology". Tutor: Prof. C. Miceli, University of Camerino. 1993/1997 Visiting Research Assistant. Tutor: Prof H.W. Detrich III, Department of Biology, 01-07/15-12 Northeastern University, Boston-MA, USA. 1995 PhD award. University of Tor Vergata, Roma, Italy. 08-08-1998 11-1998/062000 2000/2003 Post-doctoral course. Tutor: Dr. R.Melki. Laboratoire d'Enzymologie et Biochimie Structurales. CNRS, Gif-Sur-Yvette, France Researcher/Fellowship. Member of the Prof. C. Miceli’s group. University of Camerino. COURSES, STAGES; CAMPAIGNS. ERASMUS intensive program: "Blaise Pascal" University, Clermont 13-03/03-04-1994 Ferrand. France. "EMBO practical course on Cytoskeleton Dynamics" Institute of 9/19-09-1996 Molecular Biology, Austrian Academy of Sciences, Salzburg, Austria.. Antarctic field campaign at Palmer Station, Antarctica. 28-03/10-05-1997 Stage in Dr. R.Melki’s group, Laboratoire d'Enzymologie et Biochimie 17/25-03-2006 and Structurales, CNRS, Gif-Sur-Yvette, France. 25-04/1-05-2007 Projects (last 4 years): 1-COST Action BM1102 (2011-2015): Ciliates as model systems to study genome evolution, mechanisms of non-Mendelian inheritance, and their roles in environmental adaptation. 2-PNRA of the Italian Ministry of University and Research “Genome scanning and characterization of novel antifreeze proteins for industrial application” (2013-2016) 3-Principal Investigator of the MSCA-RISE MeTABLE “Advanced bioinformatics for genome and metagenome analyses and discovery of novel biocatalysts from extremophiles: implications for improving industrial bioprocesses” (2014-2018) CO-ORGANIZER OF THE TRAINING-SCHOOL/WORKSHOP: "Bioinformatics approaches to adaptations, genome evolution and biological associations", Computer Laboratory, University of Cambridge, 23th-27th March 2015. METABLE/COST ACTION BM1102 PUBLICATIONS 1) Pucciarelli S, RR Devaraj, A. Mancini, M. Castelli, P Ballarini, M Schrallhammer, G. Petroni, C Miceli (2015). Microbial consortium associated with the Antarctic marine ciliate Euplotes focardii: an investigation from genomic sequences. Microbial Ecology, Feb 14. 2) M Taffi, N Paoletti, P Lio, S Pucciarelli, M Marini (2015) Bioaccumulation modelling and sensitività analysis for discovering key players in contaminated food webs: The case study of PCBs in the Adriatic Sea. Ecological Modelling. Jen 14. 3) Ferro D, Bakiu R, De Pittà C, Boldrin F, Cattalini F, Pucciarelli S, Miceli C, Santovito G. Cu,Zn Superoxide Dismutases from Tetrahymena thermophila: Molecular Evolution and Gene Expression of the First Line of Antioxidant Defenses. Protist. 2015 Feb;166(1):13145. doi: 10.1016/j.protis.2014.12.003. Epub 2014 Dec 16. 4) Taffi M, Paoletti N, Angione C, Pucciarelli S, Marini M, Lio P. (2014) Bioremediation in marine ecosystems: a computational study combining ecological modeling and flux balance analysis. Front Genet. 5:319. 5) Gentekaki E, Kolisko M, Boscaro V, Bright KJ, Dini F, Di Giuseppe G, Gong Y, Miceli C, Modeo L, Molestina RE, Petroni G, Pucciarelli S, Roger AJ, Strom SL, Lynn DH. Largescale phylogenomic analysis reveals the phylogenetic position of the problematic taxon Protocruzia and unravels the deep phylogenetic affinities of the ciliate lineages. Mol Phylogenet Evol. 2014 Sep;78:36-42. 6) Ghazal, P., Corsi, M., Roth, A., Faggioni, F., Corti, C., Merlo Pick, E., Pucciarelli, S., Ciccocioppo, R., Ubaldi, M. Paradoxical response to the sedative effects of diazepam and alcohol in C57BL/6J mice lacking the neuropeptide S receptor (2014) Peptides, 61, pp. 107113. 7) Pucciarelli, S., Chiappori, F., Devaraj, R.R., Yang, G., Yu, T., Ballarini, P., Miceli, C. Identification and analysis of two sequences encoding ice-binding proteins obtained from a putative bacterial symbiont of the psychrophilic Antarctic ciliate Euplotes focardii (2014) Antarctic Science, 26 (5), pp. 491-501. 8) Chiellini C, Gori R, Tiezzi A, Brusetti L, Pucciarelli S, D'Amato E, Chiavola A, Sirini P, Lubello C, Petroni G. Ozonation effects for excess sludge reduction on bacterial communities composition in a full-scale activated sludge plant for domestic wastewater treatment. Environ Technol. 2014 May-Jun;35(9-12):1462-9. 9) Pucciarelli, S., Chiappori, F., Sparvoli, D., Milanesi, L., Miceli, C., Melki, R. Tubulin folding: The special case of a beta-tubulin isotype from the Antarctic psychrophilic ciliate Euplotes focardii (2013) Polar Biology, 36 (12), pp. 1833-1838. 10) Yang G, Yang G, Aprile L, Turturo V, Pucciarelli S, Pucciarelli S, Miceli C. (2013) Characterization and comparative analysis of psychrophilic and mesophilic alpha-amylases from Euplotes species: a contribution to the understanding of enzyme thermal adaptation. Biochem Biophys Res Commun. Sep 6;438(4):715-20. 11) Yang G, De Santi C, de Pascale D, Pucciarelli S, Pucciarelli S, Miceli C. (2013) Characterization of the first eukaryotic cold-adapted patatin-like phospholipase from the psychrophilic Euplotes focardii: Identification of putative determinants of thermaladaptation by comparison with the homologous protein from the mesophilic Euplotes crassus. Biochimie. Sep;95(9):1795-806. 12) Pucciarelli S, Ballarini P, Sparvoli D, Barchetta S, Yu T, Detrich HW 3rd, Miceli C. Distinct functional roles of β-tubulin isotypes in microtubule arrays of Tetrahymena thermophila, a model single-celled organism. PLoS One. 2012;7(6):e39694. doi: 10.1371/journal.pone.0039694. Epub 2012 Jun 22. PubMed PMID: 22745812; PubMed Central PMCID: PMC3382179. 13) Chiappori F, Pucciarelli S, Merelli I, Ballarini P, Miceli C, Milanesi L.Structural thermal adaptation of β-tubulins from the Antarctic psychrophilic protozoan Euplotes focardii. Proteins. 2012 Apr;80(4):1154-66. doi: 10.1002/prot.24016. Epub 2012 Jan 24. PubMed PMID: 22275059. 14) Yu T, Barchetta S, Pucciarelli S, La Terza A, Miceli C. A Novel RobustHeat-inducible Promoter for Heterologous Gene Expression in Tetrahymena thermophila. Protist. 2011 Jul 29. 15) Pucciarelli S, La Terza A, Ballarini P, Barchetta S, Yu T., Marziale F, Passini V, Methé B, Detrich HW III, Miceli C. (2009) Molecular cold-adaptation of protein function and gene regulation: The case for comparative genomic analyses in marine ciliated protozoa. Marine Genomics. 2(1), pp. 57-66 16) Marziale F., Pucciarelli S., Ballarini P., Melki R, Detrich H.W. and Miceli C. “Characterization of gamma-tubulin isotypes from the Antarctic ciliate Euplotes focardii”. FEBS J, 275: 5367-5382 17) Pucciarelli S., Buonanno F., Pellegrini G., Pozzi S., Miceli C. (2008) Biomonitoring of Lake Garda: Identification of ciliate species and symbiotic algae responsible for the “blackspot” bloom during the summer of 2004. Environmental research. 107: 194-200. 18) Barchetta S., La Terza A., Ballarini P., Pucciarelli S., Miceli. (2008) A combination of two regulatory elements in the Tetrahymena thermophila HSP70-1 gene controls heat shock activation. Eukaryotic cell. 7(2):379-86. 19) Joachimiak E., Pucciarelli S., Ballarini P., Kaczanowska J., Miceli (2007) C. Cell-cycle dependent expression of γ-tubulin in the amicronuclear ciliate Tetrahymena pyriformis. Protist. 158(1):39-50 20) Pucciarelli S., Parker S., Detrich H.W. and Melki R. (2006) Cytoplasmic chaperonin containing tcp-1 (CCT) from the Antarctic fish Notothenia coriiceps: functional and structural properties. Extremophiles. 10(6):537-49 21) Pucciarelli S., Marziale F., Di Giuseppe G., Barchetta S., Miceli C. (2005) Ribosomal coldadaptation: characterization of the acidic ribosomal P0 and P2 proteins from the Antarctic ciliate Euplotes focardii. Gene. 360: 103-11 22) Pucciarelli S., Marziale F., Ballarini P., Miceli C. (2005) Microtubule cold-stability in the Antarctic ciliate Euplotes focardii. Polarnet Technical Report. Proceedings of the Fifth PNRA Meeting on Antarctic Biology 29-30 April 2004, Messina, pp 34-38 23) Bedini R., Pucciarelli S., Gatta R., Nannelli A., Miceli C. (2004). HSP70 gene expression in sipunculids: a new biomarker for monitoring marine deposits Chemistry and Ecology, Vol. 20 (Supplement 1), pp. S345–S352. 24) Pucciarelli S., Miceli C., Melki R. (2002) Heterologous expression and folding analysis of the Antarctic ciliate Euplotes focardii beta-tubulin. Eur J Biochem. 269(24):6271-7. 25) Pucciarelli S., Miceli C. (2002) Microtubule cold adaptation: characterization of alphatubulin from the Antarctic ciliate Euplotes focardii. Extremophiles. 6(5):385-9. 26) Miceli C., Pucciarelli S., Ballarini P., Luporini P. (2000) Cold-stable microtubules of the Antarctic ciliate ciliate Euplotes focardii. In Antarctic Ecosystem. (eds Davison W., Howard-Williams C., Broady P.) Christchurch: Canterbury University Press. 154-7 27) Wirz A, Pucciarelli S, Miceli C, Tongiorgi P, Balsamo M. (1999). Novelty in phylogeny of gastrotricha: evidence from 18S rRNA gene. Mol Phylogenet Evol. 13:314-8. 28) Wirz A., Fregni E., Pucciarelli S., Miceli C., Balsamo M. (1998) Pylum Gastrotricha: a new phylogenetic perspective from molecular analysis. Atti 1° Colloquio Nazionale di Sistematica Clastidistica, Verona, Vol. 1: 63-65 29) Pucciarelli S. (1998) Characterization of tubulins and microtubules from the Antarctic ciliate Euplotes focardii. PhD thesis. 30) Pucciarelli S., Ballarini P., Miceli C. (1997) Cold-adapted microtubules: characterization of tubulin posttranslational modifications in the Antarctic ciliate Euplotes focardii. Cell Motil. Cytoskel. 38:329-40. 31) Miceli C., Pucciarelli S., Ballarini P., Valbonesi A., Luporini P. (1996) The beta-tubulin gene family of the Antarctic ciliate Euplotes focardii: determination of the complete sequence of beta-T1 gene. In Battaglia B. and Walton J. (eds) "Antarctic Communities". London: Cambridge University Press, pp. 300-306. 32) Pucciarelli S., Ballarini P., Miceli C., Luporini P. (1996). Posttranslational tubulin modifications of the Antarctic ciliate Euplotes focardii. Proceeding of the third meeting on Antarctic Biology, Santa Margherita Ligure, pp 311-315. 33) Miceli C., Pucciarelli S., Di Giuseppe G. (1995) Characterization of the beta-tubulin gene family in the Antarctic ciliate Euplotes focardii. Proceedings of the II European Congress of Protistology and VIII European Conference on Ciliate Biology, Clermond-Ferrand, pp 7175.