GIUSEPPE D`ORAZIO, PhD - BTBS - University of Milano

Dichiarazione sostitutiva di certificazione e dichiarazione sostitutiva dell’atto di notorietà ai sensi del D.P.R. 445/28.12.2000
Il/La sottoscritto/a D'Orazio Giuseppe nato a Bollate il 17/03/1984 residente in Via Garibaldi 52 – 20021 Bollate (MI) consapevole delle
responsabilità penali cui può andare incontro in caso di dichiarazioni mendaci, ai sensi e per gli effetti di cui all’art. 76 del D.P.R. 445/2000 e
sotto la propria responsabilità dichiara ai sensi dell’art. 46 e 47 del DPR 445/2000
GIUSEPPE D’ORAZIO, PhD
Home address: Via Garibaldi 52, 20021 – Bollate (MI)
Place of birth: Bollate (MI) - Date of birth: March 17th, 1984
Phone: +393491306073
e-mail [email protected]
Nationality: Italian
WORK AND RESEARCH EXPERIENCES
From January, 2013: Post-doc Researcher, Organic Chemistry laboratory of Dept. Of Biotechnology
and Biosciences – University of Milano Bicocca (Milan, Italy). Research topics: development and
synthesis of bioactive molecules with pharmacological interest as potential antitumor, antiinflammatory and antidiabetic drugs. Development of innovative materials, coatings and
formulations for probiotic-based functional foods. Development of glyco-functionalized polymeric
nanoparticles as theragnostic agents and chemical tools in biomedicine. Laboratory assistant, with
teaching support, of the Organic Chemistry laboratory course – Degree programs in Biological
Science e Biotechnologies of University of Milano Bicocca.
January 2012 – december 2013: Scientific analysis and expert advice for Eisai s.r.l. Techinical –
scientific analysis on stability of drug combination with HPLC-UV techniques.
January 2012 – december 2013: Industrial Biotechnology PhD student and laboratory assistant,
Dept. Of Biotechnology and Biosciences – University of Milano Bicocca (Milan, Italy). Development
and synthesis of compounds with potential pharmacological activity with organic and bio-organic
techniques. In vitro activity tests and enzymatic assays execution. Laboratory assistant, with
teaching support, of the Organic Chemistry laboratory course – Degree programs in Biological
Science e Biotechnologies of University of Milano Bicocca.
August – September 2012 – Visiting PhD student - Visiting PhD Researcher – CICbiomaGUNE (San
Sebastián, Spain). Preparation, purification and characterization of gold nanoparticles functionalized
with bioactive compounds within the COST Network European Program.
February – December 2007 - Research Fellowship with research activities within the Global Grants
"Ingenio", promoted by Regione Lombardia. Research project: "Food and environmental
traceability". Detection of nutraceutical compounds in food products by analytical techniques (HPLC
and LC / MS).
June – July 2002 – Internship - Laboconsult S.r.l. (Via Don Minzoni, 9 Milano). Chemical analysis of
environmental media (analysis of water, ground samples, waste). Chemical tests on environmental,
using analytical procedures concerning the processing of samples, spectrophotometric analysis in the
UV - VIS spectroscopy and atomic absorption.
EDUCATION AND TRAINING
PhD in Industrial Biotecnology achieved on February 6th, 2013 - University of Milano Bicocca (Milan,
Italy). PhD thesis and research project: “Glycoderivatives: drug candidates and molecular tools” –
Scientific supervisors: Prof. Barbara La Ferla, PhD – Prof. Francesco Nicotra.
Second Level Degree in Industrial Biotechnology - 16/10/2009 - University of Milano Bicocca (Milan,
Italy). Degree mark: 110/110 cum laude. Degree thesis: "Modulators of the Sodium Glucose Cotransporter (SGLT1) and their high anti-inflammatory activity". Supervisor: Dr. Barbara La Ferla Organic Chemistry researcher and teacher.
First Level Degree in Biotechnology - 04/12/2006 - University of Milano Bicocca (Milan, Italy). Degree
mark: 110/110 cum laude. Degree thesis: “Studies on synthesis of iminosugars analogues of natural
origin as glycosidase inhibitors". Supervisor: Prof. Laura Cipolla. Organic Chemistry researcher and
teacher.
Secondary school certificate - Chemical - Biological laboratory technician - 26/06/2003 - I.T.C.S.
“Primo Levi” (Via Varalli 20, Bollate (MI)). Final mark: 100/100 with commendation of the committee.
LANGUAGE SKILLS
English (Speaking and writing) – Level: Good. In 2012 I achieved a certification of English language
knowledge, issued by the PhD School in Science of University of Milano Bicocca. Level: C1 (Advance
English). In 2001 I attended an English language course, organized by ISIS Oxford School of English
(Oxford (UK)). My working and training experiences led to the frequent use of the English language,
in particular during National, European and International conferences and meeting.
COMPUTER SKILLS AND KNOWLEDGES
Good knowledge and use of MS Office ® (Word, Excel, PowerPoint, Access, Outlook), Windows®
operating system. Good knowledge and use of scientific applications SciFinder®, ChemBiooffice®,
Reaxys®, MestreNova®, Cresset Spark®, Schrödinger Maestro®. In 2002, I obtained a European
Computer Driving Licence.
PERSONAL AND SOCIAL SKILLS
Excellent team spirit and communication skills acquired in training and professional contexts. Good
organizational skills and aptitude for project management acquired in professional and training
experiences.
TECHNICAL SKILLS AND COMPETENCES
Good theoretical and practical knowledge of analytical chemistry techniques (HPLC / UV, LC-MS).
Good theoretical and practical knowledge of techniques for chemical characterization (NMR, UV and
Mass Spectrometry) and techniques and principles of Organic Synthesis and Medicinal Chemistry.
Good knowledge of principles and methods of Industrial Biotechnology, Industrial Biochemistry and
Enzymology.
ADDITIONAL INFORMATION
Participation in the "Course for the management and the exploitation of academic and industrial
research", University of Milano - Bicocca (Milan, Italy), May - June 2007.
Participation in the “Course of information about chemical risks”, University of Milano – Bicocca,
Milan (Italy), June 3rd, 2009.
Scientific publications on international peer-reviewed journals, book chapters and patents:
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Giuseppe D’Orazio, Laura Colombo, Mario Salmona and Barbara La Ferla, Synthesis and preliminary biological
evaluation of Fluorescent Glycofused Tricycle derivatives of Amyloid β Peptides Ligands, (Submitted for publication).
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Cristina Airoldi, Giuseppe D'Orazio, Barbara Richichi, Cinzia Guzzi, Veronica Baldoneschi, Laura Colombo, Mario
Salmona, Cristina Nativi, Francesco Nicotra, Barbara La Ferla, Structural Modifications of cis‐Glycofused Benzopyran
Compounds and Their Influence on the Binding to Amyloid‐β Peptide, Chemistry – An Asian Journal, 2015, in press DOI: 10.1002/asia.201501114.
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Di Gennaro P., D’Orazio G., La Ferla B., Labra M., Malanchin R., Piangiolino C., “Composizioni alimentari e
nutraceutiche contenenti batteri probiotici microincapsulati”, Italian Patent Apllication n° 102015000023426
(UB2015A001229), filing date: 12/06/2015.
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G D’Orazio, M Boccarusso, I Presti, V Mezzasalma, G Bizzaro, S Giardina, A Michelotti, M Labra, B La Ferla,
Microencapsulation of new probiotic formulations for gastrointestinal delivery: in vitro study to assess viability and
biological properties, Applied microbiology and biotechnology, 99, 22, 9779-9789
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Giuseppe D'Orazio, Gelsomina Parisi, Claudia Policano, Rosella Mechelli, Giovanni Codacci Pisanelli, Michele Pitaro,
Giovanni Ristori, Marco Salvetti, Francesco Nicotra, Barbara La Ferla, Arsenical C‐Glucoside Derivatives with Promising
Antitumor Activity, European Journal of Organic Chemistry, 2015, 21, 4620-4623.
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I Presti, G D’Orazio, M Labra, B La Ferla, V Mezzasalma, G Bizzaro, et. al., Evaluation of the probiotic properties of new
Lactobacillus and Bifidobacterium strains and their in vitro effect, Applied microbiology and biotechnology, Volume
99, Issue 13, pp 5613-5626.
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A Marra, R Zelli, G D'Orazio, B La Ferla, A Dondoni, Synthesis and glycosidase inhibition properties of triazole-linked
calixarene–iminosugar clusters, Tetrahedron, 70 (49), 9387-9393, 2014
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C Airoldi, S Mourtas, F Cardona, C Zona, E Sironi, G D'Orazio, et. al., Nanoliposomes presenting on surface a cisglycofused benzopyran compound display binding affinity and aggregation inhibition ability towards Amyloid β1-42
peptide, European journal of medicinal chemistry 85, 43-50, 2, 2014
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F Cardona, G D'Orazio, A Silva, F Nicotra, B La Ferla, Synthesis of glyco‐Fused Bicyclic Compounds; Conformationally
Constrained Scaffolds and Useful Polyfunctional Building Blocks, European Journal of Organic Chemistry 2014 (12),
2549-2556, 2014
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D Cardani, C Sardi, B La Ferla, G D’Orazio, M Sommariva, F Marcucci, et. al., Sodium glucose cotransporter 1 ligand
BLF501 as a novel tool for management of gastrointestinal mucositis, Molecular cancer 13 (1), 23, 2014
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B La Ferla, C Airoldi, G D'Orazio, Curcumin derivatives: Aβ-ligands as potential diagnostic and therapeutic tools for
Alzheimer disease, in Curcumin. Synthesis, Emerging Role in Pain Management and Health Implications, Daniel Loic
Pouliquen, 2014 (Book chapter)
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M Sassi, L Mascheroni, R Ruffo, MM Salamone, GA Pagani, CM Mari, G D’Orazio, B La Ferla, L Beverina, Exomethylene3, 4-ethylenedioxythiophene (emEDOT): A new versatile building block for functionalized electropolymerized poly
(3, 4-ethylenedioxythiophenes)(PEDOTs), Organic letters 15 (14), 3502-3505, 3, 2013
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C Zona, G D'Orazio, B La Ferla, Controlled-length efficient synthesis of heterobifunctionalized oligo ethylene glycols,
Synlett 24 (06), 709-712, 3, 2013
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B La Ferla, C Airoldi, C Zona, A Orsato, F Cardona, S Merlo, E Sironi, G D’Orazio, F Nicotra, Natural glycoconjugates with
antitumor activity, Natural product reports 28 (3), 630-648, 29, 2011
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B La Ferla, V Spinosa, G D'Orazio, M Palazzo, A Balsari, AA Foppoli, C Rumio, F Nicotra, Dansyl C‐Glucoside as a Novel
Agent Against Endotoxic Shock, ChemMedChem 5 (10), 1677-1680.
Oral communications in International, European and Italian meetings and symposia:
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Giuseppe D’Orazio, Patrizia Di Gennaro, Marco Boccarusso, Massimo Labra, Barbara La Ferla, Gioia Bizzaro, Silvana
Giardina, Angela Michelotti, Francesco Tursi, Marco Vassallo, PROBIOPLUS4FOOD: Characterization and
microencapsulation of new probiotic strains for their supplementation into not-diary and nutraceutical products,
31st Meeting of SIMGBM, Società Italiana di Microbiologia Generale e Biotecnologie Microbiche, Palazzo dei
Congressi – Ravenna, September 23-26, 2015.
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G. D'Orazio, “GLYCODERIVATIVES: DRUG CANDIDATES AND MOLECULAR TOOLS”, 4th Annual Meeting of the
Industrial Biotechnology PhD course of University of Milano Bicocca, Verbania (VB, Italy), October 21st – 23rd, 2012.
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Giuseppe D’Orazio, Diego Cardani, Cristiano Rumio, Francesco Nicotra, Barbara La Ferla, SGLT1-MEDIATED
ANTIINFLAMMATORY AND PROTECTIVE EFFECT OF DANSYL-GLYCODERIVATIVES. SYNTHESIS, BIOLOGICAL
EVALUATION AND STUDIES ON THE MECHANISM OF ACTION, XIII Convegno-Scuola sulla Chimica dei Carboidrati,
Pontignano (Si), June 24th – 27th, 2012.
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G. D'Orazio, “GLYCODERIVATIVES: DRUG CANDIDATES AND MOLECULAR TOOLS”, 3rd Annual Meeting of the
Industrial Biotechnology PhD course of University of Milano Bicocca, Verbania (VB, Italy), October 23rd – 25th, 2011.
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G. D’Orazio, B. La Ferla, M. Coppola, C. Magni, F. Nicotra: “Sodium-Glucose Cotransporter 1 (SGLT1) target receptor
for carbohydrate based anti-inflammatory agents”. Italian - Spanish Workshop “Chemical tools for molecular
recognition studies: Synthesis and NMR characterization of bioactive molecule”, University of Milano – Bicocca,
Milan (Italy), April 22nd, 2010.
Poster presentation:
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Giuseppe D’Orazio, Francesca Ricciardiello, Roberta Palorini, Ferdinando Chiaradonna, Barbara La Ferla, Study of the
O- and N-glycosylation profiles in cancer cell lines by modulation of the N-acetylglucosamine-based cellular
pathways, Glyco23, 23rd International Symposium of Glycoconjugates, Hotel Le Méridien-Lav, Split, September 1520, 2015.
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Giuseppe D’Orazio, Mauro Sassi, Riccardo Ruffo, Luca Beverina, Barbara La Ferla “Biosensing applications of polyethylenedioxythiophene films grafted with biological relevant molecules” XXXV Convegno della Divisione di Chimica
Organica della Società Chimica Italiana, Sassari, 9-13 September 2013.
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Giuseppe D’Orazio, Alessandro Dondoni, Alberto Marra, Barbara La Ferla. “Iminosugar-Decorated Calix[4]arenes:
Chemical Tools for the Investigation of Multivalent Inhibition of Glycosidases” XXXV Convegno della Divisione di
Chimica Organica della Società Chimica Italiana, Sassari, 9-13 September 2013.
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P. De Negri, T. Tirri, F. Peri, R. Cighetti, G. D’Orazio, Stability evaluation of Ziconotide-Morphine
HCl/Clonidine/baclofen admixtures under experimental condition, 32nd ESRA Congress, September 4-7, 2013,
Glasgow, UK.
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Barbara La Ferla, Giuseppe D’Orazio, Gabriele Tullii, Mauro Sassi, Riccardo Ruffo, Luca Beverina “Electropolymerized
poly-ethylenedioxythiophene films grafted with biological relevant molecules for biosensing applications” 18th ESOC,
July 7-12th 2013, Marseille, France.
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Giuseppe D’Orazio, Marco Marradi, Soledad Penades, Francesco Nicotra, Barbara La Ferla “Gold nanoparticles
decorated with glycomimetics as molecular tools and diagnostic-therapeutic agents”, International conference for
Nanoparticles and Nanotechnologies in Medicine NPMED 2013, 19-21 June 2013, Milan, Italy.
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Giuseppe D’Orazio, Marco Marradi, Soledad Penades, Francesco Nicotra, Barbara La Ferla “Glycomimetics conjugated
Nanoparticles: tools and diagnostic-therapeutic agents” "MultiGlycoNano" COST-Meeting 2013, 19-21 April 2013,
Prague, Austria.
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G. D'Orazio, Cardani D., Rumio C., Nicotra F., La Ferla B., SGLT1-mediated anti-inflammatory and protective effect of
dansyl-glycoderivatives. Synthesis, biological evaluation and studies on the mechanism of action, BTBS day –
Department of Biotechnology and Biosciences day – University of Milano Bicocca, November 2012.
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Giuseppe D’Orazio, Cristina Airoldi, Diego Cardani, Claudia, Cristiano Rumio, Francesco Nicotra, Barbara La Ferla. “
Synthesis and biological evaluation of ligands of SGLT1: a newly identified function for an old receptor” XXXIV
Congresso Nazionale della Divisione di Chimica Organica della Società Chimica Italiana, 10-14 September 2012,
Pavia, Italia.
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D’Orazio, G.; Zona, C.; Parisi, G.; Policano, C.; Mechelli, R.; Codacci-Pisanelli, G.; Pitaro, M.; Ristori, G.; Salvetti, M.;
Nicotra, F.; La Ferla, B.; ARSENIC C-GLYCOSIDES: APPLYING THE PAUL ERLICH’ “MAGIC BULLET” CONCEPT IN THE
TREATMENT OF CANCER, 26th International Carbohydrate Symposium (ICS2012), Madrid, July 22 nd to 27th, 2012.
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Giuseppe D’Orazio, Diego Cardani, Cristiano Rumio, Francesco Nicotra, Barbara La Ferla, SGLT1-MEDIATED
ANTIINFLAMMATORY AND PROTECTIVE EFFECT OF DANSYL-GLYCODERIVATIVES. SYNTHESIS, BIOLOGICAL
EVALUATION AND STUDIES ON THE MECHANISM OF ACTION, XIII Convegno-Scuola sulla Chimica dei Carboidrati,
Pontignano (Si), June 24th – 27th, 2012.
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Barbara La Ferla, Cristiano Zona, Giuseppe D’Orazio, Francisco Cardona, Crisitina Airoldi, Alexandre Orsato, Francesco
Nicotra, “Glycomimetics conjugated Nanoparticles with therapeutic activity and glycidic scaffold based targeting
agents”, MultiGlycoNano" - COST-Meeting 2012, Berne (Switzerland), 2nd-4th February 2012.
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G. D'Orazio, La Ferla B., Nicotra F., Glycoderivatives as drug candidates and molecular tools, BTBS day – Department
of Biotechnology and Biosciences day – University of Milano Bicocca, November 28th, 2011.
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G. D'Orazio, “GLYCODERIVATIVES: DRUG CANDIDATES AND MOLECULAR TOOLS”, 3rd Annual Meeting of the
Industrial Biotechnology PhD course of University of Milano Bicocca, Verbania (VB, Italy), October 23rd – 25th, 2011.
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Nicotra, F; La Ferla, B; D’Orazio, G; Rumio, C. “Dansyl C-Glucoside as a novel agent against endotoxic shock”. 43rd
IUPAC World Chemistry Congress, 31 July – 5 August 2011, San Juan, Puerto Rico.
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Giuseppe D’Orazio, Barbara La Ferla, Marta Coppola, Cristiano Rumio, Francesco Nicotra, “DANSYLATED CGLYCOSIDES: DRUG CANDIDATES AS ANTIIFLAMMATORY AGENTS AND MOLECULAR TOOLS FOR BIOLOGICAL
STUDIES”, 16th European Carbohydrate Symposium (EuroCarb16), Sorrento (NA, Italy), July 3rd – 7th, 2011.
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Alexandre Orsato, Giuseppe D’Orazio, Barbara La Ferla, Francesco Nicotra, “DESIGN, SYNTHESIS AND PRELIMINARY
BIOLOGICAL EVALUATION OF IMINOSUGAR-BASED POTENTIAL Akt INHIBITORS”, 16th European Carbohydrate
Symposium (EuroCarb16), Sorrento (NA, Italy), July 3 rd – 7th, 2011.
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G. D'Orazio, “GLYCODERIVATIVES: DRUG CANDIDATES AND MOLECULAR TOOLS”, 2nd Annual Meeting of the
Industrial Biotechnology PhD course of University of Milano Bicocca, Verbania (VB, Italy), October 17th – 19th, 2010.
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G. D'Orazio, B. La Ferla, M. Coppola, C. Rumio, F. Nicotra: “Design and synthesis of 2nd generation carbohydratebased antiinflammatory agents”, XXXV Summer School “A. Corbella” – Seminars in Organic Synthesis of Italian
Chemical Society, Palazzo Feltrinelli, Gargnano (BS, Italy), June 14th – 18th, 2010
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M. Barbuto, M. Casiraghi, F. De Mattia, G. D’Orazio, A. Galimberti, F. Grassi, M. Labra, B. La Ferla, A. Polissi, P.
Sperandeo: “DNA Barcoding as a multidisciplinary approach to characterize different varieties and food traceability”.
102nd Meeting of Italian Botanic Society, Palermo (Italy), September 26th – 29th, 2007.
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G. D’Orazio, B. La Ferla, P. Sperandeo, M. Labra, F. Grassi: “Studies on the determination of S-alk(en)yl-L-cysteine
sulfoxide and thiosulfinates in different species of the genus Allium”. XX National Congress of Analytical Chemistry, S.
Martino al Cimino (VT, Italy), September 16 th – 20th, 2007.
Meetings and workshop attendance:
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“Chemical Biology: Bioorthogonal Chemistry contributing to Molecular Therapeutic Innovation”, 2015 Spring OneDay Thematic Symposium of the, SCT (the French Society for Medicinal Chemistry), April 8th, 2015, Maison des
Associations Solidaires, 10 Rue des Terres au Curé, 75013 Paris (France).
“Nanomedicine Symposium CEN@Politecnico di Milano: Synthesis of nanomaterials, biological applications and
modeling” - November 14th, 2014, Politecnico di Milano, Milan (Italy).
“Hot Topics in Inflammatory Bowel Disease”, L. Sacco University Hospital, Milan, 27 th-28th January 2012.
“Laboratory of Synthetic Methodologies in Medicinal Chemistry – X edition” - University of Siena – 13th – 17th
February, 2011.
Workshop: “Glycoscience: Tools and Molecules”, Milan (Italy), December 15 th – 16th , 2010.
“New Trends in Organic Synthesis - XXV edition”, Milan (Italy), November 15th, 2010.
Workshop: “Structural Glycoscience: Methods and Instrumentation”, Grenoble (France), 29 th – July 1st, 2010.
Co-supervisor of first and second level degree thesis in Biotechnology, Industrial Biotechnology and
Chemistry – University of Milano Bicocca:
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Marzia Mancini “Nuovi approcci per l’inibizione di glicosiltransferasi: studi strutturali e funzionali” – University of
Milano Bicocca, first level degree thesis in Biotechnology, academic year 2012 – 2013.
Maria Cristina Vassallo, “Rivestimento di batteri probiotici ed analisi della stabilità in condizioni fisiologiche e in
matrici alimentari”, University of Milano Bicocca, second level degree thesis in Industrial Biotechnology, academic year
2012 – 2013.
Mariapaola Principale, “Studi sulla sintesi di nuove strutture a base glicidica con potenziale attività biologica”
Second level degree thesis in Chemistry, academic year 2012 – 2013.
Gabriele Corsaro, “Nuovi biopolimeri per interfacce neuronali”, first level degree thesis in Biotechnology, academic
year 2012 – 2013.
Alessandro Dalla Via, “Alginato: un versatile polisaccaride naturale per le biotecnologie industriali. Metodi di
incapsulazione di probiotici e immobilizazione di enzimi”, first level degree thesis in Biotechnology, academic year
2013 – 2014.
Marta Grimoldi, “Nuove evidenze sperimentali per la terapia con chaperoni farmacologici nelle malattie da
accumulo lisosomiale”, first level degree thesis in Biotechnology, academic year 2013 – 2014.
Chiara Carnesecchi, “Sintesi di tricicli glicofusi quali ligandi dei peptidi β-amiloidi”, first level degree thesis in
Chemistry, academic year 2013 – 2014.
Giulia Callegari, “Progettazione e sintesi di analoghi della N-acetil glucosammina quali potenziali agenti
antitumorali”, first level degree thesis in Chemistry, academic year 2013 – 2014.
Dario Brighetti, “Glicomimetici e glicoderivati con potenziale attività farmacologica: sintesi, caratterizzazione e studi
preliminari di attività biologica”, University of Milano Bicocca, second level degree thesis in Industrial Biotechnology,
academic year 2013 – 2014.
Federico Moretti, “Studi sulla funzionalizzazione di superfici vetrose per analisi di riconoscimento molecolare”,
Second level degree thesis in Chemistry, academic year 2013 – 2014.
Massimiliano Brivio, “Studio sulla Sintesi di Glicoderivati Triciclici”, first level degree thesis in Chemistry, academic
year 2013 – 2014.
In riferimento alla legge 196/2003 autorizzo espressamente l'utilizzo dei miei dati personali e professionali
riportati nel mio curriculum