Tematiche di ricerca Sezione Biochimica e Biologia
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Tematiche di ricerca Sezione Biochimica e Biologia
Tematiche di ricerca Sezione Biochimica e Biologia Applicate 1. 2. 3. Identificazione della funzione di geni in vari organismi. Castegna, A., Scarcia, P., Agrimi, G., Palmieri, L., Rottensteiner, H., Spera, I., Germinario, L., Palmieri, F. Identification and functional characterization of a novel mitochondrial carrier for citrate and oxoglutarate in Saccharomyces cerevisiae (2010) Journal of Biological Chemistry, 285 (23), pp. 17359-17370. Fiermonte, G., Paradies, E., Todisco, S., Marobbio, C.M.T., Palmieri, F. A novel member of solute carrier family 25 (SLC25A42) is a transporter of coenzyme A and adenosine 3′,5′-diphosphate in human mitochondria (2009) Journal of Biological Chemistry, 284 (27), pp. 18152-18159. Palmieri, L., Picault, N., Arrigoni, R., Besin, E., Palmieri, F., Hodges, M. Molecular identification of three Arabidopsis thaliana mitochondrial dicarboxylate carrier isoforms: Organ distribution, bacterial expression, reconstitution into liposomes and functional characterization (2008) Biochemical Journal, 410 (3), pp. 621-629. Identificazione di geni responsabili di patologie umane. Molinari, F., Raas-Rothschild, A., Rio, M., Fiermonte, G., Encha-Razavi, F., Palmieri, L., Palmieri, F., Ben-Neriah, Z., Kadhom, N., Vekemans, M., Attié-Bitach, T., Munnich, A., Rustin, P., Colleaux, L. Impaired mitochondrial glutamate transport in autosomal recessive neonatal myoclonic epilepsy (2005) American Journal of Human Genetics, 76 (2), pp. 334-339. Rosenberg, M.J., Agarwala, R., Bouffard, G., Davis, J., Fiermonte, G., Hilliard, M.S., Koch, T., Kalikin, L.M., Makalowska, I., Morton, D.H., Petty, E.M., Weber, J.L., Palmieri, F., Kelley, R.I., Schäffer, A.A., Biesecker, L.G. Mutant deoxynucleotide carrier is associated with congenital microcephaly (2002) Nature Genetics, 32 (1), pp. 175-179. Napolioni, V., Persico, A.M., Porcelli, V., Palmieri, L. The mitochondrial aspartate/glutamate carrier AGC1 and calcium homeostasis: Physiological links and abnormalities in autism (2011) Molecular Neurobiology, 44 (1), pp. 83-92. Studio della regolazione dell’espressione di geni in condizioni normali e patologiche. Interazione tra farmaci e fattori trascrizionali Menga, A., Infantino, V., Iacobazzi, F., Convertini, P., Palmieri, F., Iacobazzi, V. Insight into mechanism of in vitro insulin secretion increase 4. Iacobazzi, V., Convertini, P., Infantino, V., Scarcia, P., Todisco, S., Palmieri, F. Statins, fibrates and retinoic acid upregulate mitochondrial acylcarnitine carrier gene expression (2009) Biochemical and Biophysical Research Communications, 388 (4), pp. 643-647. Infantino, V., Convertini, P., Iacobazzi, F., Pisano, I., Scarcia, P., Iacobazzi, V. Identification of a novel Sp1 splice variant as a strong transcriptional activator (2011) Biochemical and Biophysical Research Communications, 412 (1), pp. 86-91. Caratterizzazione molecolare e analisi mutazionale di pazienti affetti da deficienza della traslocasi di carrier mitocondriali. 5. induced by antipsychotic clozapine: Role of FOXA1 and mitochondrial citrate carrier (2012) European Neuropsychopharmacology, . Article in Press. Infantino, V., Castegna, A., Iacobazzi, F., Spera, I., Scala, I., Andria, G., Iacobazzi, V. Impairment of methyl cycle affects mitochondrial methyl availability and glutathione level in Down's syndrome (2011) Molecular Genetics and Metabolism, 102 (3), pp. 378-382. Wibom, R., Lasorsa, F.M., Töhönen, V., Barbaro, M., Sterky, F.H., Kucinski, T., Naess, K., Jonsson, M., Pierri, C.L., Palmieri, F., Wedell, A. AGC1 deficiency associated with global cerebral hypomyelination (2009) New England Journal of Medicine, 361 (5), pp. 489-495. Edvardson, S., Porcelli, V., Jalas, C., Soiferman, D., Kellner, Y., Shaag, A., Korman, S.H., Pierri, C.L., Scarcia, P., Fraenkel, N.D., Segel, R., Schechter, A., Frumkin, A., Pines, O., Saada, A., Palmieri, L., Elpeleg, O. Agenesis of corpus callosum and optic nerve hypoplasia due to mutations in SLC25A1 encoding the mitochondrial citrate transporter (2013) Journal of Medical Genetics. Article in Press. Lindhurst, M.J., Fiermonte, G., Song, S., Struys, E., De Leonardis, F., Schwartzberg, P.L., Chen, A., Castegna, A., Verhoeven, N., Mathews, C.K., Palmieri, F., Biesecker, L.G. Knockout of Slc25a19 causes mitochondrial thiamine pyrophosphate depletion, embryonic lethality, CNS malformations, and anemia (2006) Proceedings of the National Academy of Sciences of the United States of America, 103 (43), pp. 15927-15932. Analisi molecolare di pazienti affetti da malattie genetiche autosomiche recessive: chetoadipato acidemia, microcefalia di Amish, sindrome HHH e citrullinemia di tipo II. Tessa, A., Fiermonte, G., Dionisi-Vici, C., Paradies, E., Baumgartner, M.R., Chien, Y.-H., Loguercio, C., De Baulny, H.O., Nassogne, M.-C., Schiff, M., Deodato, F., Parenti, G., Rutledge, S.L., Antonia Vilaseca, M., Melone, M.A.B., Scarano, G., Aldamiz-Echevarria, L., Besley, G., Walter, 6. 7. J., Martinez-Hernandez, E., Hernandez, J.M., Pierri, C.L., Palmieri, F., Santorelli, F.M. Identification of novel mutations in the SLC25A15 Gene in hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome: A clinical, molecular, and functional study (2009) Human Mutation, 30 (5), pp. 741-748. Fiermonte, G., Parisi, G., Martinelli, D., De Leonardis, F., Torre, G., Pierri, C.L., Saccari, A., Lasorsa, F.M., Vozza, A., Palmieri, F., Dionisi-Vici, C. A new Caucasian case of neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD): A clinical, molecular, and functional study (2011) Molecular Genetics and Metabolism, 104 (4), pp. 501-506. Fiermonte, G., Soon, D., Chaudhuri, A., Paradies, E., Lee, P.J., Krywawych, S., Palmieri, F., Lachmann, R.H. An adult with type 2 citrullinemia presenting in Europe (2008) New England Journal of Medicine, 358 (13), pp. 1408-1409. Molinari, F., Kaminska, A., Fiermonte, G., Boddaert, N., Raas-Rothschild, A., Plouin, P., Palmieri, L., Brunelle, F., Palmieri, F., Dulac, O., Munnich, A., Colleaux, L. Mutations in the mitochondrial glutamate carrier SLC25A22 in neonatal epileptic encephalopathy with suppression bursts (2009) Clinical Genetics, 76 (2), pp. 188-194. 11. Ruolo dello stress ossidativo nella distrofia muscolare e nelle cardiomiopatie Castegna, A., Palmieri, L., Spera, I., Porcelli, V., Palmieri, F., Fabis Pedrini, M.J., Kean, R.B., Barkhouse, D.A., Curtis, M.T., Hooper, D.C. Oxidative stress and reduced glutamine synthetase activity in the absence of inflammation in the cortex of mice with experimental allergic encephalomyelitis (2011) Neuroscience, 185, pp. 97-105. Palmieri, L., Alberio, S., Pisano, I., Lodi, T., Meznaric-Petrusa, M., Zidar, J., Santoro, A., Scarcia, P., Fontanesi, F., Lamantea, E., Ferrero, I., Zeviani, M. Complete loss-of-function of the heart/musclespecific adenine nucleotide translocator is associated with mitochondrial myopathy and cardiomyopathy (2005) Human Molecular Genetics, 14 (20), pp. 3079-3088. Studio dei meccanismi patogenetici di patologie umane in modelli cellulari Marobbio, C.M.T., Pisano, I., Porcelli, V., Lasorsa, F.M., Palmieri, L. Rapamycin reduces oxidative stress in frataxin-deficient yeast cells (2012) Mitochondrion, 12 (1), pp. 156-161. Galassi, G., Lamantea, E., Invernizzi, F., Tavani, F., Pisano, I., Ferrero, I., Palmieri, L., Zeviani, M. Additive effects of POLG1 and ANT1 mutations in a complex encephalomyopathy (2008) Neuromuscular Disorders, 18 (6), pp. 465-470. 8. Mutagenesi sito diretta dei trasportatori mitocondriali e relazione struttura-funzione dei carrier mitocondriali 9. Agrimi, G., Brambilla, L., Frascotti, G., Pisano, I., Porro, D., Vai, M., Palmieri, L. Deletion or overexpression of mitochondrial NAD + carriers in Saccharomyces cerevisiae alters cellular NAD and ATP contents and affects mitochondrial metabolism and the rate of glycolysis (2011) Applied and Environmental Microbiology, 77 (7), pp. 2239-2246. Ingegneria metabolica di lieviti e altri microrganismi applicata al miglioramento di processi biotecnologici di interesse industriale 11. Palmieri, F., Pierri, C.L. Mitochondrial metabolite transport. (2010) Essays in biochemistry, 47, pp. 37-52. Palmieri, F., Pierri, C.L. Structure and function of mitochondrial carriers Role of the transmembrane helix P and G residues in the gating and transport mechanism (2010) FEBS Letters, 584 (9), pp. 1931-1939. Palmieri, F., Pierri, C.L., De Grassi, A., Nunes-Nesi, A., Fernie, A.R. Evolution, structure and function of mitochondrial carriers: A review with new insights (2011) Plant Journal, 66 (1), pp. 161-181. Falconi, M., Chillemi, G., Di Marino, D., D'Annessa, I., Morozzo Della Rocca, B., Palmieri, L., Desideri, A. Structural dynamics of the mitochondrial ADP/ATP carrier revealed by molecular dynamics simulation studies (2006) Proteins: Structure, Function and Genetics, 65 (3), pp. 681-691. Studio dei network cellulari e ruolo del trasporto mitocondriale nella regolazione dei flussi metabolici di Saccharomyces cerevisiae. 10. Fontanesi, F., Palmieri, L., Scarcia, P., Lodi, T., Donnini, C., Limongelli, A., Tiranti, V., Zeviani, M., Ferrero, I., Viola, A.M. Mutation in AAC2, equivalent to human adPEO-associated ANT1 mutations, lead to defective oxidative phosphorylation in Saccharomyces cerevisiae and affect mitochondrial DNA stability (2004) Human Molecular Genetics, 13 (9), pp. 923-934. Agrimi, G., Pisano, I., Palmieri, L., Process development and metabolic engineering for bioethanol production from lignocellulosic biomass (2012) In: Biorefinery. p. 207-230, BERLINO:de Gruyter, ISBN: 978-3-11026028-1, doi: 10.1515/9783110260281.207 Modellistica delle macromolecole biologiche e metodi computazionali per la predizione della funzione di una proteina, analisi comparativa di sequenze amminoacidiche, predizione di siti di binding, screening virtuale di librerie chimiche basato sul docking molecolare e dinamica molecolare. 12. Pierri, C.L., Parisi, G., Porcelli, V. Computational approaches for protein function prediction: A combined strategy from multiple sequence alignment to molecular docking-based virtual screening (2010) Biochimica et Biophysica Acta - Proteins and Proteomics, 1804 (9), pp. 1695-1712. Sonnante, G., D'Amore, R., Blanco, E., Pierri, C.L., de Palma, M., Luo, J., Tucci, M., Martin, C. Novel hydroxycinnamoyl-coenzyme a quinate transferase genes from artichoke are involved in the synthesis of chlorogenic acid (2010) Plant Physiology, 153 (3), pp. 1224-1238. De Paola, D., Blanco, E., Pierri, C.L., Sonnante, G. Isolation and characterization of novel variants of BBI coding genes from the legume Lathyrus sativus (2012) Plant Physiology and Biochemistry, 57, pp. 4553. Metodi ab initio per lo studio del folding di proteine. Utilizzo di modelli proteici semplificati e reticoli tridimensionali. 13. 14. Pierri, C.L., De Grassi, A., Turi, A. Lattices for ab initio protein structure prediction (2008) Proteins: Structure, Function and Genetics, 73 (2), pp. 351-361. Relazione tra variabilità e funzione del genoma umano con approcci di bioinformatica e sequenziamento massivo. De Grassi, A., Ciccarelli, F.D. Tandem repeats modify the structure of human genes hosted in segmental duplications (2009) Genome Biology, 10 (12), art. no. R137, . De Grassi, A., Segala, C., Iannelli, F., Volorio, S., Bertario, L., Radice, P., Bernard, L., Ciccarelli, F.D. Ultradeep sequencing of a human ultraconserved region reveals somatic and constitutional genomic instability (2010) PLoS Biology, 8 (1), art. no. e1000275. Stranger, B.E., Forrest, M.S., Dunning, M., Ingle, C.E., Beazlsy, C., Thorne, N., Redon, R., Bird, C.P., De Grassi, A., Lee, C., Tyler-Smith, C., Carter, N., Scherer, S.W., Tavaré, S., Deloukas, P., Hurles, M.E., Dermitzakis, E.T. Relative impact of nucleotide and copy number variation on gene phenotypes (2007) Science, 315 (5813), pp. 848853. Incorporazione di peptidi e proteine in membrane modello 15. Meleleo, D., Galliani, A., Notarachille, G. AβP1-42 incorporation and channel formation in planar lipid membranes: the role of cholesterol and its oxidation products (2013) Journal of Bioenergetics and Biomembranes, pp. 1-13. Article in Press. Meleleo, D., Gallucci, E., Notarachille, G., Sblano, C., Schettino, A., Micelli, S. Studies on the effect of salts on the channel activity of kissper, a kiwi fruit peptide (2012) Open Nutraceuticals Journal, 5 (SUPPL.1), pp. 136-145. Micelli, S., Meleleo, D., Picciarelli, V., Gallucci, E. Effect of Sterols on β-Amyloid Peptide (AβP 1-40) Channel Formation and their Properties in Planar Lipid Membranes (2004) Biophysical Journal, 86 (4), pp. 2231-2237. Studio delle patologie degenerative su base infiammatoria. Infantino, V., Convertini, P., Cucci, L., Panaro, M.A., Di Noia, M.A., Calvello, R., Palmieri, F., Iacobazzi, V. The mitochondrial citrate carrier: A new player in inflammation (2011) Biochemical Journal, 438 (3), pp. 433-436. Cianciulli, A., Calvello, R., Cavallo, P., Dragone, T., Carofiglio, V., Panaro, M.A. Modulation of NF-κB activation by resveratrol in LPS treated human intestinal cells results in downregulation of PGE2 production and COX-2 expression (2012) Toxicology in Vitro, 26 (7), pp. 1122-1128. Panaro, M.A., Cianciulli, A. Current opinions and perspectives on the role of immune system in the pathogenesis of Parkinson's disease (2012) Current Pharmaceutical Design, 18 (2), pp. 200-208. Calvello, R., Panaro, M.A., Carbone, M.L., Cianciulli, A., Perrone, M.G., Vitale, P., Malerba, P., Scilimati, A. Novel selective COX-1 inhibitors suppress neuroinflammatory mediators in LPS-stimulated N13 microglial cells (2012) Pharmacological Research, 65 (1), pp. 137-148. Panaro, M.A., Carofiglio, V., Acquafredda, A., Cavallo, P., Cianciulli, A. Anti-inflammatory effects of resveratrol occur via inhibition of lipopolysaccharide-induced NF-κB activation in Caco-2 and SW480 human colon cancer cells (2012) British Journal of Nutrition, 108 (9), pp. 1623-1632.